#METABOLOMICS WORKBENCH staruschenkolab_20240731_081631 DATATRACK_ID:5064 STUDY_ID:ST003380 ANALYSIS_ID:AN005536 PROJECT_ID:PR002096
VERSION             	1
CREATED_ON             	August 6, 2024, 3:31 pm
#PROJECT
PR:PROJECT_TITLE                 	Deletion of Kcnj16 altered transcriptomic and metabolomic profiles of Dahl
PR:PROJECT_TITLE                 	salt-sensitive rats
PR:PROJECT_TYPE                  	Untargeted metabolomic analysis
PR:PROJECT_SUMMARY               	The objective of the project was to compare the metabolomic profiles in the
PR:PROJECT_SUMMARY               	kidney, urine and plasma of SSWT and SSKcnj16-/- rats. Untargeted metabolomic
PR:PROJECT_SUMMARY               	analysis revealed different metabolic profiles between SSWT and SSKcnj16-/-
PR:PROJECT_SUMMARY               	rats, with 219, 790, and 31 differentially expressed metabolites in plasma,
PR:PROJECT_SUMMARY               	urine, and kidney in SSKcnj16-/- rats compared to SSWT rats.
PR:INSTITUTE                     	University of South Florida
PR:DEPARTMENT                    	Molecular Pharmacology and Physiology
PR:LABORATORY                    	Alexander Staruschenko
PR:LAST_NAME                     	Xu
PR:FIRST_NAME                    	Biyang
PR:ADDRESS                       	560 channelside drive, Tampa, FLORIDA, 33602, USA
PR:EMAIL                         	bxu@usf.edu
PR:PHONE                         	(332) 201-4356
#STUDY
ST:STUDY_TITLE                   	Deletion of Kcnj16 altered transcriptomic and metabolomic profiles of Dahl
ST:STUDY_TITLE                   	salt-sensitive rats
ST:STUDY_TYPE                    	Untargeted metabolomis
ST:STUDY_SUMMARY                 	The inwardly rectifying K+ Channel Kir5.1 (Kcnj16) is essential in renal salt
ST:STUDY_SUMMARY                 	handling and blood pressure control. However, the underlying mechanisms are not
ST:STUDY_SUMMARY                 	fully understood. Here, we integrated transcriptomics and metabolomics to
ST:STUDY_SUMMARY                 	comprehensively profile the changes in genes and metabolites in the Dahl
ST:STUDY_SUMMARY                 	salt-sensitive (SS) rat lacking Kcnj16 to identify potential mechanisms.
ST:STUDY_SUMMARY                 	Consistent with the phenotype of knock-out (KO) rats, the transcriptomic profile
ST:STUDY_SUMMARY                 	predicted reduced blood pressure, kidney damage, and increased ion transport.
ST:STUDY_SUMMARY                 	Canonical pathway analysis suggested activation of metabolic-related while
ST:STUDY_SUMMARY                 	suppression of immune responses-related pathways in KO rats. Untargeted
ST:STUDY_SUMMARY                 	metabolomic analysis revealed different metabolic profiles between WT and KO
ST:STUDY_SUMMARY                 	rats. Integration of transcriptomic and metabolomic profiles suggested altered
ST:STUDY_SUMMARY                 	tricarboxylic acid (TCA) cycle, amino acid, and reactive oxygen species (ROS)
ST:STUDY_SUMMARY                 	metabolism that are related to SS hypertension. In conclusion, besides increased
ST:STUDY_SUMMARY                 	ion transport, our data suggest suppressed immune responses-related and altered
ST:STUDY_SUMMARY                 	metabolic-related pathways of SS rats lacking Kir5.1.
ST:INSTITUTE                     	University of South Florida
ST:DEPARTMENT                    	Molecular Pharmacology and Physiology
ST:LABORATORY                    	Alexander Staruschenko
ST:LAST_NAME                     	Xu
ST:FIRST_NAME                    	Biyang
ST:ADDRESS                       	560 channelside drive Tampa FL 33602
ST:EMAIL                         	bxu@usf.edu
ST:PHONE                         	3322014356
ST:NUM_GROUPS                    	2
ST:TOTAL_SUBJECTS                	11
ST:NUM_MALES                     	11
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Rattus norvegicus
SU:TAXONOMY_ID                   	10116
SU:GENDER                        	Male
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	s.9052.01	Sample source:plasma | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-01.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-01.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-01.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-01.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.02	Sample source:plasma | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-02.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-02.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-02.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-02.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.03	Sample source:plasma | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-03.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-03.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-03.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-03.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.04	Sample source:plasma | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-04.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-04.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-04.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-04.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.05	Sample source:plasma | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-05.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-05.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-05.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-05.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.06	Sample source:plasma | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-06.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-06.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-06.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-06.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.07	Sample source:plasma | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-07.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-07.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-07.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-07.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.08	Sample source:plasma | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-08.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-08.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-08.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-08.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.09	Sample source:plasma | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-09.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-09.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-09.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-09.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.10	Sample source:plasma | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-10.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-10.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-10.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-10.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.11	Sample source:plasma | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-28mar23-ms9052-11.d; RAW_FILE_NAME(nC18 filename)=nC18-31mar23-ms9052-11.d; RAW_FILE_NAME(philic filename)=philic-28mar23-ms9052-11.d; RAW_FILE_NAME(nhilic filename)=nhilic-29mar23-ms9052-11.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.12	Sample source:Urine | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-12.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-12.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-12.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-12.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.13	Sample source:Urine | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-13.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-13.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-13.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-13.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.14	Sample source:Urine | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-14.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-14.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-14.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-14.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.15	Sample source:Urine | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-15.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-15.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-15.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-15.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.16	Sample source:Urine | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-16.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-16.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-16.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-16.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.17	Sample source:Urine | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-17.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-17.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-17.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-17.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.18	Sample source:Urine | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-18.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-18.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-18.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-18.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.19	Sample source:Urine | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-19.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-19.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-19.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-19.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.20	Sample source:Urine | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-20.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-20.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-20.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-20.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.21	Sample source:Urine | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-21.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-21.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-21.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-21.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.22	Sample source:Urine | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-03apr23-ms9052-22.d; RAW_FILE_NAME(nC18 filename)=nC18-05apr23-ms9052-22.d; RAW_FILE_NAME(philic filename)=philic-30mar23-ms9052-22.d; RAW_FILE_NAME(nhilic filename)=nhilic-31mar23-ms9052-22.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.23	Sample source:kidney cortex | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-23.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-23.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-23.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-23.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.24	Sample source:kidney cortex | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-24.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-24.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-24.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-24.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.25	Sample source:kidney cortex | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-25.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-25.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-25.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-25.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.26	Sample source:kidney cortex | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-26.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-26.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-26.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-26.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.27	Sample source:kidney cortex | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-27.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-27.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-27.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-27.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.28	Sample source:kidney cortex | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-28.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-28.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-28.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-28.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.29	Sample source:kidney cortex | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-29.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-29.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-29.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-29.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.30	Sample source:kidney cortex | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-30.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-30.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-30.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-30.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.31	Sample source:kidney cortex | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-31.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-31.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-31.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-31.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.32	Sample source:kidney cortex | Genotype:control	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-32.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-32.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-32.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-32.d
SUBJECT_SAMPLE_FACTORS           	-	s.9052.33	Sample source:kidney cortex | Genotype:KO	RAW_FILE_NAME(pC18 filename)=pC18-06apr23-ms9052-33.d; RAW_FILE_NAME(nC18 filename)=nC18-07apr23-ms9052-33.d; RAW_FILE_NAME(philic filename)=philic-03apr23-ms9052-33.d; RAW_FILE_NAME(nhilic filename)=nhilic-04apr23-ms9052-33.d
#COLLECTION
CO:COLLECTION_SUMMARY            	Flash frozen kidney tissue, urine and plasma from arterial blood were collected
CO:SAMPLE_TYPE                   	urine, plasma, kidney cortex
#TREATMENT
TR:TREATMENT_SUMMARY             	NA
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Kidney: 10 uL/mg 1X PBS was added to the pulverized kidney tissue followed by
SP:SAMPLEPREP_SUMMARY            	vortexing to form homogenous mixture. 50 uL of homogenate was used. 500 ng Ring
SP:SAMPLEPREP_SUMMARY            	13C6-Phe IS (internal standard) were added to all samples and sonicated in ice
SP:SAMPLEPREP_SUMMARY            	bath for 30 seconds x 4 times. 300 uL 50x50 MeOH: ACN mixture were then added
SP:SAMPLEPREP_SUMMARY            	and incubated on ice for 30 min with occasional vortexing (every 10 min). The
SP:SAMPLEPREP_SUMMARY            	mixture were then centrifuged at 18,000 g for 20 min at 4oC, and dry under N2
SP:SAMPLEPREP_SUMMARY            	stream on cold blocks. Urine: 50 uL urine sample was used. 500 ng Ring 13C6-Phe
SP:SAMPLEPREP_SUMMARY            	IS (internal standard) were added to all samples and sonicated in ice bath for
SP:SAMPLEPREP_SUMMARY            	30 seconds x 4 times. 300 uL 50x50 MeOH: ACN mixture were then added and
SP:SAMPLEPREP_SUMMARY            	incubated on ice for 30 min with occasional vortexing (every 10 min). The
SP:SAMPLEPREP_SUMMARY            	mixture were then centrifuged at 18,000 g for 20 min at 4oC, and dry under N2
SP:SAMPLEPREP_SUMMARY            	stream on cold blocks. Plasma: 25 uL of plasma was used. 500 ng Ring 13C6-Phe IS
SP:SAMPLEPREP_SUMMARY            	(internal standard) were added to all samples and sonicated in ice bath for 30
SP:SAMPLEPREP_SUMMARY            	seconds x 4 times. 150 uL 50x50 MeOH: ACN mixture were then added and incubated
SP:SAMPLEPREP_SUMMARY            	on ice for 30 min with occasional vortexing (every 10 min). The mixture were
SP:SAMPLEPREP_SUMMARY            	then centrifuged at 18,000 g for 20 min at 4oC, and dry under N2 stream on cold
SP:SAMPLEPREP_SUMMARY            	blocks.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH HILIC (150 x 2.1mm,1.7um)
CH:SOLVENT_A                     	Water with 1% acetonitrile, 5mM ammonium acetate & 0.1% formic acid
CH:SOLVENT_B                     	Water with 95% acetonitrile, 5mM ammonium acetate & 0.1% formic acid
CH:FLOW_GRADIENT                 	0 min, 100% B; 1 min, 100% B; 5 min, 90% B; 13.0 min, 0% B; 16 min, 0% B; 16.5
CH:FLOW_GRADIENT                 	min, 100% B; and 20 min, 100% B
CH:FLOW_RATE                     	400 uL/min
CH:COLUMN_TEMPERATURE            	50
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Agilent 6550 QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	For each column, the run time is 20 min using a flow rate of 400 μL/min. A
MS:MS_COMMENTS                   	total of four samples per run was performed to give maximum coverage of
MS:MS_COMMENTS                   	metabolites. Samples were injected in duplicate or triplicate, and a quality
MS:MS_COMMENTS                   	control sample, made up of a subset of samples from the study was injected
MS:MS_COMMENTS                   	several times during a run. All raw data files obtained were converted to
MS:MS_COMMENTS                   	compound exchange file format using Masshunter DA reprocessor software (Agilent,
MS:MS_COMMENTS                   	USA). Mass Profile Professional (Agilent, USA) was used for data alignment and
MS:MS_COMMENTS                   	to convert each metabolite feature (m/z x intensity x time) into a matrix of
MS:MS_COMMENTS                   	detected peaks for compound identification. Components that were matched were
MS:MS_COMMENTS                   	further examined by comparison to a purchased reference standard of the proposed
MS:MS_COMMENTS                   	compound. Mass accuracy of the Q-TOF method was <5ppm with retention time
MS:MS_COMMENTS                   	precision better than 0.2%. A 1.2x fold change can be detected with a precision
MS:MS_COMMENTS                   	of 4%.
MS:MS_RESULTS_FILE               	ST003380_AN005536_Results.txt	UNITS:peak area	Has m/z:Yes	Has RT:No	RT units:No RT data
#END