#METABOLOMICS WORKBENCH jgengatharan_20240802_084702 DATATRACK_ID:5077 STUDY_ID:ST003384 ANALYSIS_ID:AN005542 PROJECT_ID:PR002085 VERSION 1 CREATED_ON August 2, 2024, 8:54 am #PROJECT PR:PROJECT_TITLE Altered sphingolipid biosynthetic flux and lipoprotein trafficking contribute to PR:PROJECT_TITLE trans fat-induced atherosclerosis PR:PROJECT_SUMMARY The goal of the project is to determine the role of sphingolipid metabolism in PR:PROJECT_SUMMARY atherosclerosis induced by dietary trans fat. We analyzed lipid metabolites in PR:PROJECT_SUMMARY Huh7 cells following various fatty acid treatments, with specific focus on cis PR:PROJECT_SUMMARY and trans unsaturated fatty acids. Additionally, we analyzed lipid metabolites PR:PROJECT_SUMMARY in plasma and liver of Ldlr-/- mice fed high-fat diets enriched in cis or trans PR:PROJECT_SUMMARY fatty acids in the presence or absence of myriocin, a pharmacological inhibitor PR:PROJECT_SUMMARY of SPT, the initial rate-limiting enzyme of sphingolipid biosynthesis. PR:INSTITUTE Salk Institute for Biological Studies PR:LAST_NAME Gengatharan PR:FIRST_NAME Jivani PR:ADDRESS 10010 N Torrey Pines Rd, La Jolla, CA, 92037, USA PR:EMAIL jivani14@gmail.com PR:PHONE (858) 453-4100 #STUDY ST:STUDY_TITLE Impact of high-fat diet enriched in cis or trans fatty acids and myriocin on ST:STUDY_TITLE plasma lipidome in Ldlr-/- mice ST:STUDY_SUMMARY We analyzed LPC, LPE, PC, PE, DG, TG, and CE in the plasma of Ldlr-/- mice fed ST:STUDY_SUMMARY 1) Cis HFD, 2) Cis HFD + Myriocin, 3) Trans HFD, or 4) Trans HFD + Myriocin. We ST:STUDY_SUMMARY aimed to determine how dietary cis and trans monounsaturated fatty acids impact ST:STUDY_SUMMARY the broader plasma lipidome while pharmacologically inhibiting the initial ST:STUDY_SUMMARY rate-limiting enzyme of sphingolipid biosynthesis, serine palmitoyltransferase ST:STUDY_SUMMARY (SPT), via myriocin. ST:INSTITUTE Salk Institute for Biological Studies ST:LAST_NAME Gengatharan ST:FIRST_NAME Jivani ST:ADDRESS 10010 N Torrey Pines Rd, La Jolla, CA, 92037, USA ST:EMAIL jivani14@gmail.com ST:PHONE (858) 453-4100 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_11 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_11.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_12 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_12.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_13 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_13.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_14 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_14.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_15 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_15.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_31 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_31.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_32 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_32.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_33 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_33.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_34 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_34.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFD_35 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFD_35.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_101 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_101.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_102 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_102.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_103 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_103.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_104 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_104.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_105 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_105.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_141 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_141.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_142 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_142.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_143 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_143.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_144 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_144.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_CisHFDMyr_145 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_CisHFDMyr_145.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_41 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_41.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_42 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_42.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_43 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_43.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_44 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_44.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_45 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_45.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_81 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_81.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_82 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_82.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_83 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_83.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_84 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_84.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFD_85 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFD_85.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_61 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_61.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_62 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_62.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_63 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_63.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_64 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_64.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_65 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_65.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_91 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_91.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_92 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_92.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_93 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_93.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_94 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_94.mzXML SUBJECT_SAMPLE_FACTORS - Plasma_C30_TransHFDMyr_95 Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin RAW_FILE_NAME(Raw file name)=Plasma_C30_TransHFDMyr_95.mzXML #COLLECTION CO:COLLECTION_SUMMARY Blood was collected in EDTA-coated tubes (Sarstedt Inc.) and centrifuged at CO:COLLECTION_SUMMARY 2000g for 5 min. The supernatant was transferred to a new Eppendorf tube and CO:COLLECTION_SUMMARY stored at -80°C until analysis. CO:SAMPLE_TYPE Liver #TREATMENT TR:TREATMENT_SUMMARY Four-five-week-old Ldlr-/- C57BL/6J male and female mice (JAX# 002207) were fed TR:TREATMENT_SUMMARY with irradiated 60% high fat diets (HFD) prepared by Dyets for 16 weeks. These TR:TREATMENT_SUMMARY diets include Cis Unsaturated HFD (105063GI), Cis Unsaturated HFD with 2.2 mg/kg TR:TREATMENT_SUMMARY Myriocin Added (105064GI), Trans Unsaturated HFD (105061GI), and Trans TR:TREATMENT_SUMMARY Unsaturated HFD with 2.2 mg/kg Myriocin Added (105061GI). The Trans Unsaturated TR:TREATMENT_SUMMARY HFD was designed with 100% Primex, a partially hydrogenated vegetable oil, and TR:TREATMENT_SUMMARY the Cis Unsaturated HFD was designed with 34% lard and 66% olive oil. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Plasma (10 µl) was spiked with 1 ug of each of the following internal SP:SAMPLEPREP_SUMMARY standards: 18:1-d7 cholesteryl ester (Avanti Polar Lipids, Cat #791645), SP:SAMPLEPREP_SUMMARY 15:0-18:1(d7) phosphatidylcholine (Avanti Polar Lipids, Cat #791637), SP:SAMPLEPREP_SUMMARY 15:0-18:1(d7) phosphatidylethanolamine (Avanti Polar Lipids, Cat #791638), SP:SAMPLEPREP_SUMMARY 18:1(d7) lysophosphatidylcholine (Avanti Polar Lipids, Cat#791643), 18:1(d7) SP:SAMPLEPREP_SUMMARY lysophosphatidylethanolamine (Avanti Polar Lipids, Cat #791644), 15:0-18:1(d7) SP:SAMPLEPREP_SUMMARY diacylglycerol (Avanti Polar Lipids, Cat #791647), 15:0-18:1(d7)-15:0 SP:SAMPLEPREP_SUMMARY triacylglycerol (Avanti Polar Lipids, Cat #791648). 0.5 mL methanol, 0.5 mL H2O, SP:SAMPLEPREP_SUMMARY and 1 mL chloroform were added directly. Samples were vortexed for 5 min, SP:SAMPLEPREP_SUMMARY centrifuged for 5 min at 4 ˚C at 15,000g. The organic phase was collected and 2 SP:SAMPLEPREP_SUMMARY μL of formic acid was added to the remaining polar phase which was re-extracted SP:SAMPLEPREP_SUMMARY with 1 mL of chloroform. Combined organic phases were dried under nitrogen.After SP:SAMPLEPREP_SUMMARY dried extracts for plasma were resuspended in 60 µl 65:30:5 ACN: IPA:H2O, 5 µL SP:SAMPLEPREP_SUMMARY of sample was injected. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Thermo Accucore C30 (150 x 2.1mm,2.6um) CH:SOLVENT_A 60% acetonitrile, 40% water; 0.1% formic acid; 10 mM ammonium formate CH:SOLVENT_B 90% isopropanol, 10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate CH:FLOW_GRADIENT 0 min, 30% B; 3 min, 30% B; 8 min, 43% B; 9 min, 50% B; 18 min, 90% B; 26 min, CH:FLOW_GRADIENT 99% B; 30 min, 99%B; 36 min, 30% B CH:FLOW_RATE 0.2 mL/min CH:COLUMN_TEMPERATURE 40˚C #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS Lipids were identified in EL-MAVEN using exact mass of precursor ion in MS1 MS:MS_COMMENTS chromatogram and product ion in MS2 spectra. Lipids were quantified using peak MS:MS_COMMENTS areas in MS1 chromatogram. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak area MS_METABOLITE_DATA_START Samples Plasma_C30_CisHFD_11 Plasma_C30_CisHFD_12 Plasma_C30_CisHFD_13 Plasma_C30_CisHFD_14 Plasma_C30_CisHFD_15 Plasma_C30_CisHFD_31 Plasma_C30_CisHFD_32 Plasma_C30_CisHFD_33 Plasma_C30_CisHFD_34 Plasma_C30_CisHFD_35 Plasma_C30_CisHFDMyr_101 Plasma_C30_CisHFDMyr_102 Plasma_C30_CisHFDMyr_103 Plasma_C30_CisHFDMyr_104 Plasma_C30_CisHFDMyr_105 Plasma_C30_CisHFDMyr_141 Plasma_C30_CisHFDMyr_142 Plasma_C30_CisHFDMyr_143 Plasma_C30_CisHFDMyr_144 Plasma_C30_CisHFDMyr_145 Plasma_C30_TransHFD_41 Plasma_C30_TransHFD_42 Plasma_C30_TransHFD_43 Plasma_C30_TransHFD_44 Plasma_C30_TransHFD_45 Plasma_C30_TransHFD_81 Plasma_C30_TransHFD_82 Plasma_C30_TransHFD_83 Plasma_C30_TransHFD_84 Plasma_C30_TransHFD_85 Plasma_C30_TransHFDMyr_61 Plasma_C30_TransHFDMyr_62 Plasma_C30_TransHFDMyr_63 Plasma_C30_TransHFDMyr_64 Plasma_C30_TransHFDMyr_65 Plasma_C30_TransHFDMyr_91 Plasma_C30_TransHFDMyr_92 Plasma_C30_TransHFDMyr_93 Plasma_C30_TransHFDMyr_94 Plasma_C30_TransHFDMyr_95 Factors Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Cis HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin Genotype:Ldlr-/- | Sample source:Mouse plasma | Group:Trans HFD + Myriocin LPC 18:1-d7 15816985600 18693484544 16883889152 17550938112 17479612416 15890347008 17720070144 16754237440 19837628416 20347557888 18147303424 20424237056 20023539712 19454851072 20879099904 18016415744 19331272704 20510019584 20508002304 0 17130204160 19687751680 19321438208 18611726336 18731872256 18363568128 19558350848 19831615488 19608348672 19545831424 7665804800 22059368448 19728867328 21606103040 19112652800 21024886784 20522930176 19288598528 22245818368 20599513088 LPC 14:0 402956064 146812512 250400480 208947200 271210112 257829264 129146928 181942640 174585248 161322224 107434480 140925216 165241056 175159760 144707888 131029544 176890576 188324128 201034928 0 207339824 187943456 176767760 180116032 197322736 182221456 180582944 213016704 229450096 246273664 60228640 173267296 176738192 194525520 163954656 140477568 166664336 182395744 134970672 192476784 LPC 16:0 1.65714E+11 88311627776 1.24908E+11 1.15994E+11 1.36694E+11 1.2925E+11 62374977536 97127538688 81005051904 89899073536 40319619072 39130755072 38901858304 77495828480 65804705792 64511836160 84342841344 86208036864 73015205888 0 71147921408 55713890304 71580942336 76539764736 68203884544 72313716736 48407273472 85772869632 90475012096 1.01687E+11 16259987456 45573554176 49994952704 50537463808 45609418752 46136680448 46725693440 55131447296 40587476992 50490748928 LPC 16:1 4822980608 1546134400 2947035136 2601273856 3221366016 3438618880 1253748864 2083492992 1610810496 1653253504 854571200 724187648 803560256 1745460992 1409634944 1262688768 1694539904 1876402048 2027318272 0 6533629952 4070049792 5177101312 5383458816 5506462720 5337268224 3639253504 6472339968 6333925376 6260800512 1446761472 4588468224 4690279936 5014910976 4362973696 4005736448 4285092352 4892490240 4110195712 4659268608 LPC 18:0 1.52189E+11 79773589504 1.16028E+11 1.17679E+11 1.31622E+11 1.23948E+11 59870347264 87205412864 79224594432 86310510592 30659248128 34821009408 33233037312 67197579264 58884100096 55612456960 72319541248 75125997568 62345003008 0 51652694016 33341616128 45906599936 55307501568 51912986624 55397392384 35435536384 67502657536 72438677504 76630392832 8566745088 27613851648 32420634624 33062268928 28088518656 26937415680 31694696448 37158100992 23153567744 36692721664 LPC 18:1 77756628992 37032796160 52737527808 53942693888 62258614272 59006124032 33192869888 44104253440 38907871232 38428446720 19383130112 15744145408 17068658688 35129155584 29851910144 28763445248 34715791360 37475205120 39627243520 0 67209228288 53815091200 55366901760 60867813376 58624720896 63353819136 48769228800 74440998912 83264724992 82445402112 15684247552 46652825600 51426988032 55041798144 46117457920 44214685696 48275460096 57036460032 45315407872 54185656320 LPC 18:2 53341429760 27643619328 33608603648 31348396032 36467105792 39809863680 27475980288 35102248960 37993824256 27951067136 11525048320 10162990080 11191771136 19499165696 16932982784 18375954432 22143154176 21593778176 25811623936 0 38440501248 26687924224 23845732352 26789210112 30069510144 29734377472 22696595456 41887465472 42109485056 40409595904 9086521344 25041905664 29481469952 32514523136 25000882176 26040018944 27326212096 31064666112 26865164288 29186697216 LPC 18:3 297001728 130313792 128451760 115656016 117230304 185830672 228494752 201578640 227060416 160123360 151381248 228380448 218812992 167933776 181590864 116771520 130519672 179014432 256086752 0 316111776 295348576 226011168 173091088 226609232 175501280 324510656 259225120 279588416 258250208 159171136 381425024 365609696 421050784 345336000 404087264 358273888 321495520 448413376 344119008 LPC 20:0 1524492672 1243407360 1192698624 1598851456 1101714560 1265724288 1245626368 1457982464 1358532736 1300797824 477766016 297878816 278063520 1082342912 912450816 1192662912 1069567104 1219876480 1295283968 177004160 925258176 245660224 530629344 480598688 673262208 705827648 358226784 998318528 742715264 569021120 106909744 300779328 369798080 358190976 345689440 374199744 373831552 412719936 306784832 381182560 LPC 20:1 2489879808 1088522368 1778270080 1855104384 1941765120 1995348096 922111104 1322633472 1148990976 1311953664 492807744 355959296 351919648 1247490176 1050158400 781948544 1063608192 1029402304 1226038912 0 2049092224 985533952 1531544064 1641461248 1760109696 1887474816 1102038016 2352614144 2349479424 1966354432 318253152 958902400 1116574592 1246530688 996002112 917109248 1013212608 1257195904 828776960 1224335360 LPC 20:2 1327249920 536808256 801485888 820463808 956737280 1012962816 499835264 690807616 691367616 628143616 209935424 154829008 175146400 493545824 389464960 344417472 474353664 428593248 568017920 805.95 888183936 606227776 722771008 801625088 817663936 923385472 503886240 1119399168 1317943040 1176429696 164579440 485217600 581223936 674522368 499467168 462271296 490808000 642786560 406246272 587980480 LPC 20:3 11878324224 4753494016 6101634048 7423271424 8097001472 8899861504 3761252352 6217462272 5135270400 5384214016 995697792 528524512 663176768 2922104832 2235734272 2098437248 2978212096 2770845952 3303144960 0 3957877504 2522777856 3105322240 3162633728 3402935808 3589476608 2144390528 4454118912 5113192448 4842723840 770626880 1924635136 2319799040 2707049216 2065836032 2185806080 2045311616 2499748352 1697942016 2280824320 LPC 20:4 35862417408 14492772352 23170707456 18458621952 27954268160 26372016128 13424793600 17429303296 17621972992 16729662464 7476112896 4258096896 5550611968 14778626048 11891270656 8633261056 15328577536 14742895616 14240083968 0 11491532800 10948336640 10118950912 10640788480 9939686400 10762518528 8311072768 10864091136 13267992576 13500830720 2394547968 7346735104 7536458752 9486788608 7054675968 7564070912 7317145600 7925858304 5977690112 8425332224 LPC 22:0 542125824 340577216 476132096 479355680 447407168 442176544 347197280 371588096 368278016 345581440 175267232 244773056 234890768 350556864 300213088 296551328 378311904 386074912 344356224 132472064 315075968 202935792 231272880 250784128 291437632 318487072 226098368 401350720 401137824 366680800 72930896 193992528 240999776 229687744 200219520 169247184 208673696 266188496 168748528 240140064 LPC 22:1 233199776 133063016 167361872 183660064 173942960 170189312 122574240 151019120 152019952 143485376 78211936 94002552 87507520 164811152 141248464 115605960 150692016 165232064 160863632 58215752 170811648 102589008 112071264 112955168 132273600 147844848 119070768 184876816 176999088 149426032 38368640 116899184 136033280 134444064 115127776 105343080 127157048 150116416 113206576 140854576 LPC 22:3 45758360 19003920 27374464 30103554 33431986 34772476 17400696 21366314 21384820 18782680 8609068 6932867.5 8119434.5 19274052 14062400 11403255 18261980 20546322 19411732 0 26716704 34911088 25321806 23518676 24815792 28302564 26755460 30401108 40630172 36540524 6411414.5 18598142 23700896 26238806 20495846 21102854 22161570 26733662 17618024 26279790 LPC 22:4 306230208 195994400 231027536 241179328 226901376 257455504 156261376 193277568 186651344 184833152 87339000 54145184 66268420 150471584 125510312 111496480 157963456 158494848 163777072 0 165089216 159300624 139364816 139168736 145769952 169897504 120960816 184837664 211048288 194030048 40604196 115080680 135038864 148418320 107490704 124634192 129269496 146091104 110607712 145575200 LPC 22:5 1521037824 689374720 1053505024 807321856 955325056 1110359040 630532608 698152064 760019840 612093248 328872128 271986368 327604960 702531200 392476928 370645568 690546880 716648832 688466368 677.02 1141751296 1494061696 978588800 1039868992 1023621440 1199460864 1044882048 1182986880 1502905344 1240622080 250399472 648800576 775459584 909334784 676460544 811882176 764566784 912762112 607954432 874893504 LPC 22:6 12469288960 7619286016 8097139712 8147176448 7727718912 8477861888 5372426240 6720809472 7423051776 6293072896 2875963392 830287488 1100791040 5105363456 4442926080 3859694080 5596908032 5015053312 5410081792 0 2027641600 1106880640 1345443584 1465448320 1483434880 1712298496 956431488 1935810816 1983577344 1760518528 471694304 1301272320 1480511872 1761945472 1231034112 1397704960 1483977344 1563277440 1198827648 1440852480 LPC 24:0 719045184 495637568 717186624 589464064 656861888 623686784 445909088 549586048 535633024 507889600 258401264 384180000 367861088 559604800 485596416 412375584 578748864 557813568 471393440 190241696 392617472 326279360 383323168 403767520 389171168 472161216 328755200 510623936 580248512 551674560 123083192 301470528 368336384 335676800 309966016 238658320 294948096 410560672 261742736 372830336 LPC 24:1 271443104 155377920 219670464 221871856 226180112 207560864 139084912 173991536 152909792 159726464 84926112 136072160 125636928 179049568 158931536 117397008 170326912 167375536 153943840 73128936 172050240 135190416 136646720 141266016 155865376 179476608 146876976 211778048 226590400 206653680 49655280 135106480 173812912 166598736 136054720 120355248 154890128 188317728 134703712 182014048 LPE 18:1-d7 6647717376 7005248000 6583479296 7368268288 6800679936 6469136384 6520538112 6270863360 6560209408 7452430336 6460410368 7137473024 6903472128 7387700736 7556481024 6654424576 6798127104 7456510464 7279216128 0 6142920704 7189892608 7004646912 7098072576 6815080448 7225972736 6706757632 7275488256 7950703616 7578856448 2852304896 7364786176 6972993024 7688616448 6651634688 7424505344 7240126464 7309935616 8147500032 7228110336 LPE 16:0 410355424 208889296 312512352 319374624 324877312 300147072 144207520 196577216 137809312 197805856 113395840 231463296 202048720 237146176 201654320 213151776 257211456 354057344 228639424 0 179842928 216370320 151583504 177842880 175429440 195484224 181698896 205686800 241611808 251235872 62839300 160733760 199405840 204314640 174328608 199598992 185327040 280017824 209080192 207856080 LPE 18:0 582248960 315660608 415140480 457382272 478297056 454097536 206304528 297358112 242392816 288136768 138810128 335160640 286269152 295636960 238692144 259295872 326670656 418471648 313612928 0 195422528 203698256 152012240 191504272 171544624 204008272 192589904 240155216 271495360 279059872 48662608 145738992 217092960 190097152 168369632 186673008 201924544 260202976 186639344 216890992 LPE 18:1 306512544 99710336 169644320 156078864 186811360 228878016 108382496 132988008 113076208 98655680 78908176 171202128 159764400 114387128 104708848 95207824 108230224 141190512 133385352 0 231550736 299568480 174586368 220209872 223519936 240650896 281796160 261172064 270979584 283512000 87307512 222647232 276935424 294097152 230811584 239731664 253524416 346771584 302829728 289484000 LPE 18:2 181507296 74233688 83467056 81289096 72581320 139525216 101519968 106334544 95884352 55096616 36411320 80828448 74419376 56128076 48182568 57365800 48099760 56209164 82647976 0 180843120 180552560 109224304 141588640 149027056 166268992 162219936 199170928 173593280 169409968 52202292 141548768 176923168 196714112 136973424 156573488 166646768 190669120 190690128 147973536 LPE 20:3 54793960 22734562 27877444 37728992 30203440 38495676 17309098 25116588 22343624 16580433 5548519.5 4640584 5275223 13931253 8557931 11886449 16296326 18903410 22367320 0 28327700 18464002 16469373 19593968 25111792 23692500 16086757 26776226 27261450 25335338 2160260 15356934 19094650 21511522 14694116 16841840 15936802 20502428 15815781 14608315 LPE 20:4 756806208 278722208 359520800 402228000 367272480 525749856 242937968 299766240 232109472 182788656 120744840 151556512 155263232 233768432 178901456 170121456 205933504 226599472 230680336 0 256373168 247813632 218121472 274422304 252892512 295775552 179974000 262710672 268623520 288030304 64138384 174434848 209419968 221320000 162335248 206419280 175095376 214299856 184726352 157141024 LPE 22:5 41453376 12761585 23799498 18777236 22580318 31412492 11991848 13201677 8543197 8208574 6903831.5 16326118 15360912 16903044 9889008 7642389 12692815 18100704 14850957 0 45933064 68266304 45529548 56037452 45772400 61893468 38500380 45294428 52147812 47126284 10488012 26460586 32724864 35372032 27343640 34536860 27558628 35769356 26949858 27595548 LPE 22:6 670637888 298281056 366045952 399524960 318134976 441188480 206820992 267104592 195161584 175068560 119509576 71347768 76730840 230001216 183800560 182108816 216805232 235969120 227963248 0 106445320 63872100 84055808 102314360 96433392 118675584 50190264 95950144 91862840 92674936 25669924 68691848 81905776 84327464 63748184 77802552 69555880 78956496 66948884 59789528 PC 15:0/18:1-d7 22246965248 36417273856 30860611584 30025811968 27722749952 26353995776 39441211392 30447030272 39861051392 36178870272 53280460800 50405548032 53323796480 43896528896 48304050176 43292442624 40830955520 41459326976 43002667008 59473428480 20941064192 29977288704 27192922112 26222151680 24313417728 23823687680 30006034432 20593068032 21924892672 20934017024 21659346944 36050042880 31987292160 33770407936 33331333120 34492313600 32226850816 28971722752 35023056896 32261332992 PC 32:0 9976812544 7917314048 10525636608 10058952704 9992623104 9748149248 7909786624 8535412224 8155776000 8528325120 7437552640 15288645632 14632750080 9311309824 9469078528 7910500864 10328077312 9304640512 8255084544 11020024832 5647783424 9636371456 8002603008 6627517440 6985715712 6288109568 8662702080 6418775552 7258640384 7698899968 4246342400 6680365056 7507992064 6594488320 7119128576 5405281280 6527092224 7255402496 5562656768 8222889472 PC 32:1 2630580480 2121255808 2504024064 2750809600 2500952832 2571157504 2335827968 2368867328 1814090880 2075408256 1780938752 3235521792 2854004736 2378989824 2267487744 2205669120 2250376704 2827718656 2667517952 2430974720 4785661952 5588574720 5855775232 5425738752 5463773696 5296044544 5422244864 5896814080 5914598912 6358674432 3740893440 5976896512 6388081152 5694544384 5946905600 5328380416 5924985344 6486302720 5323966464 6165007360 PC 32:2 1138820224 605987520 765943104 897969792 888654848 978900160 754537024 829078528 645841728 620331712 328577792 474236000 420204672 604566208 511416320 592474944 632452800 890695296 906017856 318631936 2715800320 2060550784 2522213632 2223142656 2519037184 2537123072 1909666688 3276869376 3028119040 3060582144 800724224 2152520192 2347692544 2224090880 2094592768 2025463808 2402505984 2722317056 2337917184 2444241920 PC 32:3 98294072 23108866 34429908 35757756 45192048 45909588 27077048 34145736 27666214 27153574 11052612 27602338 25323912 22993286 19664926 23652680 25856834 41664500 36060144 18528960 241339520 179008320 199749264 173088800 218863760 203302432 181003952 296247232 258734144 245224400 48578808 178558672 175929296 181128112 154854352 150121840 191677664 220627856 187577824 196791136 PC 34:0 19120173056 11609723904 17033677824 16472419328 19226202112 15163769856 10272468992 12819747840 12199839744 13227568128 6098673664 8176382976 8117724160 12734164992 11124554752 9874081792 13743249408 12235577344 11375598592 4380535296 6251724800 5806044160 6975726592 6640444416 6399774208 6844644352 5278321664 7538629120 8696222720 8618801152 2238712064 4478120448 5676642304 4967527424 4656287744 4129714688 4660236288 6242391040 4097316864 6572841984 PC 34:1 1.30143E+11 1.23952E+11 1.3584E+11 1.57295E+11 1.2862E+11 1.3947E+11 1.28994E+11 1.29342E+11 1.07373E+11 1.20089E+11 1.06366E+11 1.01809E+11 1.0144E+11 1.27657E+11 1.27046E+11 1.27973E+11 1.23474E+11 1.39544E+11 1.2926E+11 87502225408 1.37663E+11 1.46668E+11 1.58906E+11 1.54788E+11 1.63805E+11 1.45408E+11 1.33137E+11 1.54717E+11 1.59706E+11 1.71093E+11 98557853696 1.40333E+11 1.54251E+11 1.41978E+11 1.44009E+11 1.49329E+11 1.47525E+11 1.53666E+11 1.45576E+11 1.50824E+11 PC 34:2 1.5652E+11 1.56977E+11 1.40234E+11 1.58616E+11 1.40673E+11 1.50952E+11 1.49059E+11 1.55717E+11 1.63551E+11 1.38679E+11 1.04214E+11 1.08664E+11 1.00169E+11 1.24887E+11 1.16344E+11 1.46342E+11 1.38333E+11 1.38481E+11 1.42724E+11 83549675520 1.44204E+11 1.18874E+11 1.28861E+11 1.33049E+11 1.33973E+11 1.42069E+11 1.12754E+11 1.57392E+11 1.48213E+11 1.52278E+11 86366412800 1.19512E+11 1.29252E+11 1.23029E+11 1.17593E+11 1.2511E+11 1.29638E+11 1.39649E+11 1.26661E+11 1.23339E+11 PC 34:3 11819728896 8221188096 8940061696 10107867136 9407710208 11678987264 11036980224 11371715584 8692194304 8688763904 5278055936 5310774784 5048052736 7828543488 7606914048 8519052800 7853542400 11009707008 11200012288 4113612544 43321024512 23391825920 31175475200 28198723584 33481324544 34381357056 23009882112 41625649152 35823792128 33609054208 13120390144 29544718336 32342558720 31617853440 28915122176 31935854592 34094198784 36488245248 37411766272 31676338176 PC 34:4 1110760320 468764640 751739840 734073152 913191296 1038016192 693088640 771329920 556628864 640883328 270404192 366232448 345402400 498309632 418595840 405069696 498371680 641338560 651891520 287932352 3004141568 1731059328 2396467200 2115844736 2492844800 2617414400 1696306816 3334960384 3053506304 2829597184 776241024 1978894464 2022338560 2128078336 1898054144 1991192576 2184450304 2411271680 2327183360 2131750784 PC 34:5 148878496 69190256 104029832 119270352 127054864 126854264 75303448 92132400 66128536 82506096 28829712 37435280 32604550 67938720 60006948 55112720 62925448 70708528 83685448 18368396 456046272 363294240 439217120 389734976 464087264 467039936 369277984 575896256 556717120 502493632 131570288 366895328 411122464 425219712 404148608 365749824 396583072 465761088 393932672 422785440 PC 36:1 1.86917E+11 1.34579E+11 1.5178E+11 1.72747E+11 1.63488E+11 1.68278E+11 1.32213E+11 1.50211E+11 1.27134E+11 1.39969E+11 68438499328 1.08508E+11 1.02632E+11 1.22104E+11 1.12575E+11 1.12692E+11 1.14631E+11 1.21127E+11 1.28077E+11 69558525952 1.35869E+11 1.15718E+11 1.27285E+11 1.42777E+11 1.36839E+11 1.55743E+11 1.14914E+11 1.72214E+11 1.90123E+11 1.8532E+11 46453911552 1.02455E+11 1.26949E+11 1.20981E+11 1.03369E+11 1.08556E+11 1.1777E+11 1.49095E+11 1.13313E+11 1.40707E+11 PC 36:2 1.95852E+11 1.82243E+11 1.85851E+11 1.87845E+11 1.76612E+11 1.97948E+11 2.00067E+11 1.993E+11 2.01202E+11 1.8507E+11 1.15635E+11 1.59388E+11 1.49545E+11 1.55629E+11 1.50418E+11 1.60453E+11 1.60964E+11 1.58716E+11 1.64733E+11 1.18236E+11 2.16928E+11 1.94054E+11 1.83461E+11 1.98675E+11 1.99045E+11 2.11282E+11 1.96813E+11 2.3135E+11 2.32378E+11 2.227E+11 1.05118E+11 1.78023E+11 2.03368E+11 1.975E+11 1.80481E+11 1.81573E+11 1.99912E+11 2.20286E+11 2.02534E+11 2.07361E+11 PC 36:3 1.08874E+11 77510852608 86862422016 1.02197E+11 96412647424 1.08064E+11 77000474624 92757917696 74823385088 83273801728 37668360192 41996931072 40223838208 60382863360 55982534656 54695469056 61062184960 58509377536 61981634560 35363770368 1.39331E+11 1.21634E+11 1.13737E+11 1.19989E+11 1.24835E+11 1.28317E+11 1.23787E+11 1.39154E+11 1.35077E+11 1.2922E+11 75806728192 1.09659E+11 1.2536E+11 1.22851E+11 1.16015E+11 1.16565E+11 1.26275E+11 1.30025E+11 1.29791E+11 1.24243E+11 PC 36:4 1.09131E+11 86459752448 1.10123E+11 1.03472E+11 1.13278E+11 1.15247E+11 93903101952 98181488640 84481998848 1.06818E+11 73022078976 76016746496 84287528960 94737653760 93110247424 76485672960 93889208320 94802632704 80285949952 66890051584 76963045376 79876898816 85966372864 85150343168 85493628928 80950067200 73485803520 79136137216 84017659904 89624043520 39142137856 64957923328 67400859648 67384811520 65815429120 70186901504 67739017216 68826202112 60971388928 69939896320 PC 36:5 10525852672 4834130944 6749272064 6994891776 8555814912 8992645120 5990372864 7339652608 5204688896 5882015744 1945297280 1791190912 1933777792 4368273408 3982391296 3715024128 4510856192 5199656448 5221823488 1498354944 14117936128 8783680512 12749317120 10863733760 12176311296 12846711808 7240036864 14014862336 13990000640 14178588672 2807377920 7960847872 7819188224 8798490624 7284878848 8160864256 8354962432 9764844544 7673808896 8833881088 PC 36:6 659662976 291384128 461609120 406277824 551429760 514553536 325120320 428970304 298228448 380046656 107718688 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16017230848 16912437248 16248178688 1204547840 3636769536 5366068224 4468814848 4316284928 3795234560 4199459840 5696790528 2602226432 5062552576 TG 56:4 9571558400 5486214144 8975162368 6579482624 9579691008 9160153088 6099302912 6531582976 5774855168 6502090752 1670277248 4967754240 3000499456 3532326912 2468993024 2684328192 3788269056 5587399168 4509006848 2928835328 6668760576 9011910656 10103473152 10986242048 8150961152 8442707968 8288443904 8016284672 8816268288 9260497920 1324226688 3643376896 4914363392 4307811328 4612094976 4406867456 4327769600 5173664768 2839186688 4844538880 TG 56:5 30790285312 17306402816 27321083904 23052494848 31141881856 28197210112 16457980928 20215611392 16079446016 20614789120 4469481984 15790977024 9486413824 11789136896 7383584768 8045225984 11656496128 13744205824 11144108032 8710840320 13848707072 23340113920 21981093888 22505879552 17055825920 19292817408 21374199808 17076433920 19797319680 20740904960 2119686656 6818484736 8529613312 7883478528 8377438720 7759754240 7841480192 9809806336 4848904192 8872799232 TG 56:6 50378973184 33814067200 46665101312 42861694976 45353033728 49949249536 39506063360 39391580160 37871640576 40789880832 8059463168 24862640128 15226615808 18997596160 12429281280 15358041088 22979334144 19427899392 19921076224 12373344256 26773696512 35225022464 33791817728 33403990016 30348091392 33941448704 34249422848 32410580992 34245965824 34789404672 2831460352 8887523328 10825601024 9831550976 10236504064 9319282688 10660466688 12658956288 6213591552 12026572800 TG 56:7 48961622016 28185071616 42261364736 38661300224 43551490048 47210487808 32355188736 33465399296 29216737280 36096888832 7028722176 15032085504 8425005056 13657674752 9617948672 12540899328 15983059968 15642339328 13842601984 7358556160 18887075840 23811211264 24267382784 25003233280 22289029120 26513338368 25730375680 25994645504 26200285184 25806077952 1695975296 6438487552 7722928128 6928250368 7619585536 6780389376 7878620160 8863618048 4439562752 8393808384 TG 56:8 21855770624 10246670336 17854322688 16071912448 18628044800 20756295680 11551780864 12837798912 10305849344 14680071168 2754793216 6046862848 3343545344 4959076864 3568835072 4635218944 5793623552 5078459904 4665302528 2795401984 5999713792 6825289216 8722466816 8999276544 8791950336 9412848640 8838715392 9441988608 8892844032 8934611968 544437888 2359772672 2640194048 2293500416 2655390976 2447319296 2910683392 3031915008 1578485504 2788548608 TG 58:1 75352352 328553216 204869536 75526216 82626528 106049800 251781664 176692368 276754176 111205784 29645764 119346192 102762672 80628416 60811672 105858512 161652288 98158736 210340944 84792448 233815648 340624480 176114880 165320096 151750000 151288160 250165664 205991424 116282768 147250912 19557986 50064092 73066984 58149640 44216220 32127030 62670616 77429520 29260300 70142848 TG 58:2 598115712 1564507136 1411007104 501133760 491880384 664451968 1345424256 920505088 1231984000 694890048 118850856 483328448 293758752 321052864 238247072 441771040 726138880 564808576 749012032 250674368 2245945088 2779459328 1801545856 1736617472 1098023424 1293973632 1761395712 1811558656 1395666304 1246502528 103579336 245030928 441266688 364962272 263742032 188589840 323906368 455616064 144069872 433341600 TG 58:3 1406263424 1888800512 2125013120 1154750592 1128296064 1384127360 1909713920 1633517312 1916141440 1346309248 293579840 883121280 433654304 556851776 416933984 576117632 1008631744 1566958336 1149640064 422169088 2334282752 2707064320 2393003264 2589440768 1458414720 1666401408 1999468416 2050732928 1962198272 1691840384 117539728 324987168 610425088 467271264 402361248 351017728 445235712 571011264 249778032 550234368 TG 58:4 927213184 713389760 1000818688 683252288 831813440 890398912 802506496 788423808 711914496 727461952 176180384 531965312 288994656 337137952 240759056 317616672 463281728 716953984 597187648 314034112 1036862336 1387663104 1397231360 1558620416 954772992 1050773248 1218514176 1141918848 1263654400 1165113472 91952576 265659072 441619040 349052800 361435648 380740544 356899008 428373440 251535424 382076960 TG 58:5 3334087168 1694174336 3025321472 2093620608 3578801664 3031890944 1704519936 2022808704 1673581696 2026324480 385689504 1952972032 1108407296 1149804288 674107776 746676160 1172155776 1558769792 1339616512 1086508800 1432053120 2866762240 2664643328 2873568000 1792246784 2011153664 2477886208 1786503936 2183180800 2437187072 213760752 657255872 829274112 827931904 876126720 948134080 786983552 1018192256 533335264 875808832 TG 58:6 10511864832 4833214976 8945785856 6975271936 9833548800 9530999808 5049407488 6168851456 4872192512 6403520000 1057197440 4584272896 2584281600 3377046016 1985634816 2126334336 3251031040 3480332032 3292302336 2526535936 2997155584 5818093568 5515005952 6061529088 4132775680 4750651904 5123264512 4073464064 5058857984 5780475392 420942048 1328222592 1612260096 1602651520 1645897472 1727760768 1577458048 1969554944 1013734080 1826256512 TG 58:7 8809635840 4779872768 7610439680 6499823104 8634622976 7906094592 5058996736 5554963968 4814834176 5988539392 1670400384 7171032064 4869724672 4089067776 2504807936 2686732032 3807188480 2548738816 3735396608 4374447616 3882902016 7423835136 5640773632 5496724480 4827357696 5567157760 6550946816 4707231744 5410762240 5793860096 555483776 1517029376 1938182528 1970214016 2031630208 2006072832 1967102208 2361900544 1287973504 2204920832 TG 58:8 25908742144 13289924608 22426886144 19436007424 25309698048 24060899328 14554957824 16081881088 13415296000 18081492992 3720253696 11315337216 6864828928 8731309056 5659801600 6406111744 9056374784 6583884288 8212615680 5932676096 6365756928 9610982400 8446177280 8352199168 7701807104 8774385664 9667647488 8327753728 8746460160 9268412416 627617536 2063734016 2637330432 2543351296 2737087488 2419838464 2749408768 3144378624 1454298368 3056283136 TG 60:1 7381538 41102212 21109680 10278956 9951980 13422712 28987782 19414706 38656232 12006280 3101360.75 15370986 16476837 10720885 8002647 15770069 21788362 9720175 29459210 14317358 20336356 32211762 14726335 15389723 16381175 16025118 26232062 20560796 10700359 16303160 1750515.5 3844701.5 6607068 4069230.5 3135680 1735192.25 4624902 6697179 3437178 6070301 TG 60:2 117522584 494705824 323283072 130319920 123513720 168790208 371630272 257021088 461337120 170993888 38720960 197930608 143590352 101580672 77312112 166570048 242862032 142416512 320297856 105868848 429788896 629787520 325665344 308010528 248137552 291480832 430580992 361203616 267907872 266040592 28343416 66750204 106075808 87095224 66079208 50847512 86868000 113998736 45315532 108528952 TG 60:3 310443584 675403648 617651072 258807840 280363264 319399904 592718976 498402112 614732416 332754432 64574048 254510368 149998656 144886656 111341008 188381760 296919552 282675392 378553120 124427472 586755456 724019328 549938816 496568384 323639168 394256800 477340704 445736352 402273760 333959168 33894428 81739392 163407712 125781816 102532280 72592624 117665456 149198848 60136016 145910592 TG 60:4 126836944 171765072 207472576 97448064 111078616 126837768 167994560 144911872 168962976 112282344 11757948 110631096 67313328 50432544 32377100 51907316 95598168 91100936 107179968 59310912 232460768 298355904 244076272 232386688 141903584 177108800 225473008 189468192 181631808 155576208 10640994 34947692 63291380 52394804 41752412 38324280 55430892 60872668 28201508 56311600 TG 60:5 171227920 167338208 222823712 115522328 166814480 162508288 163686336 158040752 164892896 140265600 18929332 145764368 88203432 54793128 36295268 54991648 94088104 99579552 109773744 87272744 190857296 336398912 276857280 270456448 149425296 188283552 269445184 189888304 211792720 194221088 9822119 31958060 51984572 43349408 38864468 50429032 48763636 54929316 27571802 46702488 TG 60:6 687469184 323756192 612832256 425219968 660634432 640006656 336540864 398150976 315122464 404618432 75042952 479074688 249122864 234668912 141693696 162753536 223849024 273587712 294109408 246893040 264216928 676004416 553472000 591221120 357900384 409951520 559531264 358002624 447419424 505640896 35047700 113715880 146697248 141972768 149952352 169574160 152513440 179902272 111504680 155621760 TG 60:7 1763847296 656326656 1388612608 991077760 1704052224 1507382272 607035136 858149504 643006016 885759424 211863280 1323811712 782110784 595369536 362285888 361799776 508460192 529404736 648672704 776024704 533726912 1435991680 1129128704 1250124544 770682112 959698048 1212906880 732339072 1016981248 1213486848 71409104 239720448 294524480 291653248 304634336 344315104 291911968 357427008 214304624 348662944 TG 60:8 2049053440 847928768 1700924800 1292212352 2080191616 1698668416 795357696 1041562368 803949248 1179354240 316544288 1518400256 1000601792 782774784 518676992 517059072 677758592 474683392 790831936 957176960 477480992 1128245248 816530304 810187264 668742528 769876672 974804352 585697728 735174208 828187712 85314416 215109040 279904384 279441408 296380032 339953376 298619264 338643392 220177296 331531296 CE 18:1-d7 134231568 241825216 112942584 207248224 196785328 176234240 427535616 286159808 466285440 309866592 987157376 883760384 718725504 226205232 287245728 349395424 156113584 223772736 519501280 875323520 373978656 314169472 147472944 168963216 288306944 181307264 506694336 227279232 158963280 190821360 308960736 756399616 266471088 878580032 604678464 930206528 656132096 252015808 675394112 261230208 CE 16:0 358190496 86377800 129229160 349820672 415546976 336523840 373283360 303298528 472050656 335137280 137708224 207090064 245238208 76419800 100459440 71470464 150775568 148215120 255226672 227789792 95133360 61616984 119126168 126084560 390537536 129342672 129737968 207450160 80815496 94439016 117410016 296773184 121058056 576521920 424650112 210350608 400323616 113413544 293400800 86841216 CE 16:1 883428864 475339200 761677952 794114176 1007872640 783207616 513792352 597600832 566766976 544509376 368234976 256070704 169802336 556986560 504279456 535880224 655122688 629196096 761589120 196536832 780153408 813881856 990541696 905328064 861024768 767573504 654662848 882157888 954106496 1293040896 383549120 1039856448 760718848 1310136960 984809408 792101504 869598080 666807232 500578944 697857536 CE 18:0 9867901 3614494 1733050 9729559 13626835 10341570 16708003 14815376 32590012 9222641 12358926 30258706 41019052 1508820.62 1935122.5 4815344.5 3493802 6798052 37391020 33159204 17935284 7775097.5 3588096.75 4607880 25406538 4631282 15642744 16257863 4228670 10040388 6729391 19821486 4004567.5 33807060 26532452 17810258 35368324 4039539.25 19638298 3615895.75 CE 18:1 6005088256 4241167360 4117057024 6334990848 6700737536 5931844608 6255252480 6717775360 7334275072 5778952704 6693226496 6601082368 4833508352 3589452544 3665457152 4270129664 4083054848 4994754560 9039299584 4440677888 7257560064 5203893248 3656729088 3901574912 6134243328 4895658496 6767442944 5966140928 5114314240 7523118080 3995659264 8802735104 4274370816 11980003328 8580068352 7905177088 8453285376 4348702720 6064454656 4123793152 CE 18:2 30384128000 27678818304 27047241728 31126188032 32698216448 30355972096 33693265920 33971898368 36969832448 34852605952 33453553664 21610549248 17499486208 28494147584 28644749312 32204544000 25730891776 28465872896 35087437824 15618052096 41966235648 29105975296 30166441984 31208435712 38472044544 34687414272 33224003584 38209560576 33796179968 37094436864 22936068096 43697537024 32021868544 53393317888 39920390144 39738015744 41171566592 33051844608 31725989888 31880790016 CE 18:3 2133645312 2343149056 2143743744 2958315776 2501944576 2101501056 2186709760 2597143040 2182311168 2522141440 2418681088 1293146368 967936960 3390939392 3190786304 3330270720 2764868352 2098209152 2478907648 888174400 2797327872 1964866176 2669370880 2337967104 2602984704 2431585536 1968101760 2468586752 2441352704 2386294016 1429328128 3901332992 3229307136 3439889152 3570444800 3581210112 3521732352 3342970880 2360883968 2988014080 CE 20:1 9929433 10772576 8874983 12061852 15136143 11612383 14450334 16091898 25571854 11464839 28044088 29108930 17991670 11775769 11604041 17363950 12239928 24653500 56456556 18221162 39239312 31478118 21413258 23824644 26537390 14799861 31421562 24495560 18730388 30686294 15686570 52812588 22376328 72139392 46752580 58665936 44383360 21999938 32284452 24097430 CE 20:2 85462608 93577144 92565800 92909424 111344712 86252208 88582968 97872432 119996968 95729888 116367488 95823480 66159496 112135976 108552272 117711448 109780368 124129552 151792864 70682240 231887664 190127744 177640288 157122896 174560704 154632912 161968432 185057920 163381136 200572880 94840032 257478160 176415776 339090528 232041952 242238384 218665136 173799088 150524816 177858000 CE 20:3 6909702656 5980782592 6417998336 7602805760 8087633408 6745973760 4339980288 5956290048 4681400832 6487051776 3249351168 1890406144 1502878976 6399080960 5321478144 5602085888 5828214272 5702619648 4409288704 1506000640 5811711488 4561413632 7750149632 6918201856 5604944384 5831639552 4120778752 5583831040 5932714496 6540950528 2176385536 4796327936 4256011264 5315639296 4533392896 4690708480 4367619584 4579037696 2936603136 4404593152 CE 20:4 67895193600 58348236800 63664603136 66252677120 74969169920 63622467584 51093704704 61628096512 58904354816 67500642304 58049503232 41146077184 34175053824 69180940288 66401546240 64757932032 66897264640 62599962624 54727467008 32176525312 51511865344 45384785920 59021619200 58253615104 55306956800 55405535232 45324197888 49190494208 52334120960 56911540224 30151321600 56277217280 50066800640 54334705664 52527509504 52950671360 51853557760 52975505408 37083439104 52322889728 CE 20:5 3717640704 3761814272 3790000384 5337060864 4589089792 3284274944 2744072192 3593608960 3031946496 4125686016 2244760064 1166352512 832872320 4339545088 4972797440 4315200512 3889798400 2319420672 2676973568 774346560 999528064 561240384 1158667904 1068305792 1264772096 1046216704 579257920 843955520 871251904 1006353344 824702208 2143928704 1854669824 1881470592 1944109312 2072743680 1923272576 1920229760 1293356160 1764198528 CE 22:4 234945088 220404592 238853568 229799920 292343648 204354640 156192192 178495440 197720080 198464768 193334736 159686464 111467752 269462816 220845728 219548976 256100416 298860032 183500848 108201464 222127728 210375840 296264896 278158720 213134144 224872608 173648128 222027504 211154720 225724336 90701008 233251776 195869120 236002128 184010240 211312480 190433680 220676912 152551184 211533888 CE 22:5 3262452480 2632244224 3828328192 2886564864 3807315200 2949082112 2219687168 2424764160 2536725760 2827716608 2302019584 1711009152 1245876736 4010463488 2603356672 2885130240 3931019264 3506691840 2104658688 1026173120 5796827648 6473862144 8538268160 8152257536 6223760896 6373316608 5098679808 5199951360 6144387584 6249735168 1795344384 5551409152 4772702208 5720224768 5217878528 5253323776 4734471168 5633382400 3104660224 5748971008 CE 22:6 50414514176 47678685184 48137678848 52188463104 52083146752 45473398784 36900794368 45117014016 40599445504 48930525184 29005772800 10350405632 8090921472 45008044032 45078011904 45970419712 47975088128 38973050880 32469921792 6725120512 21026236416 11043261440 25444564992 24428124160 22851618816 22869055488 11835891712 21445611520 20919832576 23275337728 7720092672 21914015744 19560505344 20463388672 20349321216 18910615552 19922147328 22009030656 12076348416 20622399488 CE 24:3 1332294.75 876722.5 1474580.88 889564.12 1860464.88 1066485.5 1547859 1129341.25 2270240.75 835033 3852202 13230468 7075957 2751516.75 1230844.88 2012580.75 2179259.5 5576291.5 5846402 7134907 4309415.5 14756503 6538846.5 5161656 3294343 4226885.5 8660925 3064359.25 5037725 7376702.5 2355765 6806191 4698023 8109328 8045502 9538188 7941345.5 5459595 5632947 5785947.5 CE 24:4 24316866 23460674 30153760 21579820 29038520 22113716 19654928 21704328 31127200 20909040 39113292 37518652 24557746 31468908 26739788 28984658 39855536 48248144 44849752 21549102 36520600 59290328 46976184 42041472 29031342 33010036 43081972 29058610 36606364 44232164 17734044 46759968 39283388 55741344 50284652 49261944 46924348 41692956 31883026 43814252 CE 24:5 17005348 22660512 23373896 23862750 19671970 15463757 15602549 19674534 24159126 18039782 22970936 7658876.5 5309375.5 19057524 21815264 24423294 30409232 22608562 33434072 5639202 15846248 6367079 13812717 11964928 10887295 9483931 7411069.5 11445196 9173405 9296098 6700423.5 20906686 16497046 23119394 20611434 18594130 19478592 17862082 13701786 16400878 CE 24:6 65624256 76824664 87833992 90345216 69396360 62805268 60919764 67933224 62965144 78840832 45914136 23644878 16861426 87557136 82082904 87399232 92623320 69885136 68835520 14904901 61457284 31010462 53728508 52176636 53313760 51896704 27596374 46756864 45226880 44054436 27986888 75603240 62494240 77679232 71759648 74016888 71552904 77293328 50929512 69660792 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name m/z LPC 18:1-d7 529.3999 LPC 14:0 468.3084 LPC 16:0 496.3405 LPC 16:1 494.3246 LPC 18:0 524.3721 LPC 18:1 522.3566 LPC 18:2 520.3407 LPC 18:3 518.3249 LPC 20:0 552.4039 LPC 20:1 550.3877 LPC 20:2 548.3721 LPC 20:3 546.3563 LPC 20:4 544.3405 LPC 22:0 580.4346 LPC 22:1 578.4194 LPC 22:3 574.388 LPC 22:4 572.3725 LPC 22:5 570.3556 LPC 22:6 568.3405 LPC 24:0 608.4656 LPC 24:1 606.4502 LPE 18:1-d7 487.3533 LPE 16:0 454.2929 LPE 18:0 482.3247 LPE 18:1 480.3087 LPE 18:2 478.2931 LPE 20:3 504.308 LPE 20:4 502.2937 LPE 22:5 528.3093 LPE 22:6 526.2927 PC 15:0/18:1-d7 753.6151 PC 32:0 734.5704 PC 32:1 732.5546 PC 32:2 730.5389 PC 32:3 728.5238 PC 34:0 762.6011 PC 34:1 760.586 PC 34:2 758.5704 PC 34:3 756.5545 PC 34:4 754.5388 PC 34:5 752.5232 PC 36:1 788.6172 PC 36:2 786.6018 PC 36:3 784.5859 PC 36:4 782.5701 PC 36:5 780.5546 PC 36:6 778.5391 PC 36:7 776.5225 PC 36:0 790.6304 PC 38:1 816.6466 PC 38:2 814.6334 PC 38:3 812.6169 PC 38:4 810.6016 PC 38:5 808.5853 PC 38:6 806.5699 PC 38:7 804.5544 PC 38:8 802.5393 PC 40:2 842.6628 PC 40:3 840.6475 PC 40:4 838.6324 PC 40:5 836.6166 PC 40:6 834.6016 PC 40:7 832.5853 PC 40:8 830.5697 PC 42:4 866.6641 PC 42:5 864.6492 PC 42:6 862.6326 PC 42:7 860.6176 PC 42:8 858.5995 PC 42:9 856.584 PC 42:10 854.5703 PE 15:0/18:1-d7 711.567 PE 34:1 718.5397 PE 34:2 716.5229 PE 36:1 746.5696 PE 36:2 744.5558 PE 36:3 742.5398 PE 36:4 740.523 PE 36:5 738.5079 PE 38:5 766.5388 PE 38:6 764.5223 PE 38:7 762.5068 PE 40:7 790.539 PE 40:8 788.5209 DG 15:0/18:1-d7 605.5858 DG 32:0 586.5417 DG 32:1 584.5258 DG 32:2 582.5099 DG 34:0 614.5727 DG 34:1 612.5576 DG 34:2 610.5408 DG 34:3 608.5259 DG 36:0 642.6047 DG 36:1 640.589 DG 36:2 638.5724 DG 36:3 636.5574 DG 36:4 634.5411 DG 38:1 668.6195 DG 38:2 666.6041 DG 38:3 664.5876 DG 38:4 662.5729 DG 38:5 660.5569 DG 38:6 658.5408 TG 15:0/18:1-d7/15:0 829.7999 TG 48:0 824.7711 TG 48:1 822.7556 TG 48:2 820.7396 TG 48:3 818.7233 TG 50:0 852.803 TG 50:1 850.7874 TG 50:2 848.7714 TG 50:3 846.755 TG 50:4 844.7386 TG 50:5 842.7229 TG 50:6 840.7082 TG 50:7 838.6925 TG 52:0 880.8342 TG 52:1 878.8138 TG 52:2 876.7993 TG 52:3 874.7872 TG 52:4 872.7712 TG 52:5 870.7539 TG 52:6 868.7397 TG 52:7 866.7242 TG 52:8 864.7097 TG 54:1 906.8501 TG 54:2 904.834 TG 54:3 902.8186 TG 54:4 900.803 TG 54:5 898.7865 TG 54:6 896.7709 TG 54:7 894.756 TG 54:8 892.7405 TG 56:1 934.8813 TG 56:2 932.8657 TG 56:3 930.8497 TG 56:4 928.8342 TG 56:5 926.8177 TG 56:6 924.8026 TG 56:7 922.7872 TG 56:8 920.7714 TG 58:1 962.9119 TG 58:2 960.8968 TG 58:3 958.8812 TG 58:4 956.8651 TG 58:5 954.8492 TG 58:6 952.8335 TG 58:7 950.8183 TG 58:8 948.8023 TG 60:1 990.9429 TG 60:2 988.9284 TG 60:3 986.9127 TG 60:4 984.8966 TG 60:5 982.882 TG 60:6 980.8641 TG 60:7 978.8497 TG 60:8 976.8337 CE 18:1-d7 675.6782 CE 16:0 642.6198 CE 16:1 640.6038 CE 18:0 670.6506 CE 18:1 668.6349 CE 18:2 666.6195 CE 18:3 664.6035 CE 20:1 696.6658 CE 20:2 694.6506 CE 20:3 692.6348 CE 20:4 690.6194 CE 20:5 688.6036 CE 22:4 718.6506 CE 22:5 716.6349 CE 22:6 714.6194 CE 24:3 748.6976 CE 24:4 746.6824 CE 24:5 744.6667 CE 24:6 742.6507 METABOLITES_END #END