#METABOLOMICS WORKBENCH SatoH_20240801_012205 DATATRACK_ID:5070 STUDY_ID:ST003395 ANALYSIS_ID:AN005569 PROJECT_ID:PR002102
VERSION             	1
CREATED_ON             	August 8, 2024, 9:45 am
#PROJECT
PR:PROJECT_TITLE                 	Group X Phospholipase A2 Links Colonic Lipid Homeostasis to Systemic Metabolism
PR:PROJECT_TITLE                 	via Host-Microbiota Interaction
PR:PROJECT_SUMMARY               	The gut microbiota influences physiological functions of the host, ranging from
PR:PROJECT_SUMMARY               	the maintenance of local gut homeostasis to systemic immunity and metabolism.
PR:PROJECT_SUMMARY               	Secreted phospholipase A2 group X (sPLA2-X) is abundantly expressed in colonic
PR:PROJECT_SUMMARY               	epithelial cells but is barely detectable in metabolic and immune tissues.
PR:PROJECT_SUMMARY               	Despite this distribution, sPLA2-X-deficient (Pla2g10-/-) mice displayed
PR:PROJECT_SUMMARY               	variable obesity-related phenotypes that were abrogated after treatment with
PR:PROJECT_SUMMARY               	antibiotics or cohousing with Pla2g10+/+ mice, suggesting the involvement of gut
PR:PROJECT_SUMMARY               	microbiota. Under housing conditions where Pla2g10-/- mice showed aggravation of
PR:PROJECT_SUMMARY               	diet-induced obesity and insulin resistance, they displayed increased colonic
PR:PROJECT_SUMMARY               	inflammation and epithelial damage, reduced production of polyunsaturated fatty
PR:PROJECT_SUMMARY               	acids (PUFAs) and lysophospholipids, decreased abundance of several Clostridium
PR:PROJECT_SUMMARY               	species, and reduced levels of short-chain fatty acids (SCFAs). These
PR:PROJECT_SUMMARY               	obesity-related phenotypes in Pla2g10-/- mice were reversed by dietary
PR:PROJECT_SUMMARY               	supplementation with omega-3 PUFAs or SCFAs. Thus, colonic sPLA2-X orchestrates
PR:PROJECT_SUMMARY               	omega-3 PUFA-SCFA interplay via modulation of gut microbiota, thereby
PR:PROJECT_SUMMARY               	secondarily affecting systemic metabolism.
PR:INSTITUTE                     	University of Tokyo
PR:LAST_NAME                     	Sato
PR:FIRST_NAME                    	Hiroyasu
PR:ADDRESS                       	7-3-1 Hongo, Bunkyo-ku Tokyo 113-8655, Japan.
PR:EMAIL                         	sato-hr@m.u-tokyo.ac.jp
PR:PHONE                         	81-3-5841-1433
#STUDY
ST:STUDY_TITLE                   	Short chain fatty acids analysis of Feces and Plasma in Pla2g10 knockout mice.
ST:STUDY_SUMMARY                 	We analyzed short-chain fatty acids, including acetic acid, propionic acid, and
ST:STUDY_SUMMARY                 	butyric acid, in the feces and plasma of WT and Pla2g10 knockout mice that were
ST:STUDY_SUMMARY                 	fed either a low-fat diet (LFD), high-fat diet (HFD), lard diet, or omega-3
ST:STUDY_SUMMARY                 	diet.
ST:INSTITUTE                     	University of Tokyo
ST:LAST_NAME                     	Sato
ST:FIRST_NAME                    	Hiroyasu
ST:ADDRESS                       	7-3-1 Hongo, Bunkyo-ku Tokyo 113-8655, Japan.
ST:EMAIL                         	sato-hr@m.u-tokyo.ac.jp
ST:PHONE                         	81-3-5841-1433
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	LF_1	LF1	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_1.mzML
SUBJECT_SAMPLE_FACTORS           	LF_2	LF2	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_2.mzML
SUBJECT_SAMPLE_FACTORS           	LF_3	LF3	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_3.mzML
SUBJECT_SAMPLE_FACTORS           	LF_15	LF15	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_15.mzML
SUBJECT_SAMPLE_FACTORS           	LF_16	LF16	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_16.mzML
SUBJECT_SAMPLE_FACTORS           	LF_17	LF17	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_17.mzML
SUBJECT_SAMPLE_FACTORS           	LF_18	LF18	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_18.mzML
SUBJECT_SAMPLE_FACTORS           	LF_19	LF19	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_19.mzML
SUBJECT_SAMPLE_FACTORS           	HF_7	HF7	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_7.mzML
SUBJECT_SAMPLE_FACTORS           	HF_8	HF8	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_8.mzML
SUBJECT_SAMPLE_FACTORS           	HF_9	HF9	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_9.mzML
SUBJECT_SAMPLE_FACTORS           	HF_10	HF10	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_10.mzML
SUBJECT_SAMPLE_FACTORS           	HF_20	HF20	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_20.mzML
SUBJECT_SAMPLE_FACTORS           	HF_21	HF21	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_21.mzML
SUBJECT_SAMPLE_FACTORS           	HF_22	HF22	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_22.mzML
SUBJECT_SAMPLE_FACTORS           	HF_23	HF23	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_23.mzML
SUBJECT_SAMPLE_FACTORS           	LF_4	LF4	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_4.mzML
SUBJECT_SAMPLE_FACTORS           	LF_5	LF5	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_5.mzML
SUBJECT_SAMPLE_FACTORS           	LF_6	LF6	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_6.mzML
SUBJECT_SAMPLE_FACTORS           	LF_25	LF25	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_25.mzML
SUBJECT_SAMPLE_FACTORS           	LF_26	LF26	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_26.mzML
SUBJECT_SAMPLE_FACTORS           	LF_27	LF27	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_27.mzML
SUBJECT_SAMPLE_FACTORS           	LF_28	LF28	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LF_28.mzML
SUBJECT_SAMPLE_FACTORS           	HF_11	HF11	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_11.mzML
SUBJECT_SAMPLE_FACTORS           	HF_12	HF12	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_12.mzML
SUBJECT_SAMPLE_FACTORS           	HF_13	HF13	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_13.mzML
SUBJECT_SAMPLE_FACTORS           	HF_14	HF14	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_14.mzML
SUBJECT_SAMPLE_FACTORS           	HF_29	HF29	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_29.mzML
SUBJECT_SAMPLE_FACTORS           	HF_30	HF30	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_30.mzML
SUBJECT_SAMPLE_FACTORS           	HF_31	HF31	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_31.mzML
SUBJECT_SAMPLE_FACTORS           	HF_32	HF32	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=HF_32.mzML
SUBJECT_SAMPLE_FACTORS           	LP_1	LP1	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_1.mzML
SUBJECT_SAMPLE_FACTORS           	LP_2	LP2	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_2.mzML
SUBJECT_SAMPLE_FACTORS           	LP_3	LP3	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_3.mzML
SUBJECT_SAMPLE_FACTORS           	LP_15	LP15	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_15.mzML
SUBJECT_SAMPLE_FACTORS           	LP_16	LP16	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_16.mzML
SUBJECT_SAMPLE_FACTORS           	LP_17	LP17	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_17.mzML
SUBJECT_SAMPLE_FACTORS           	LP_18	LP18	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_18.mzML
SUBJECT_SAMPLE_FACTORS           	LP_19	LP19	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_19.mzML
SUBJECT_SAMPLE_FACTORS           	HP_7	HP7	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_7.mzML
SUBJECT_SAMPLE_FACTORS           	HP_8	HP8	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_8.mzML
SUBJECT_SAMPLE_FACTORS           	HP_9	HP9	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_9.mzML
SUBJECT_SAMPLE_FACTORS           	HP_10	HP10	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_10.mzML
SUBJECT_SAMPLE_FACTORS           	HP_20	HP20	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_20.mzML
SUBJECT_SAMPLE_FACTORS           	HP_21	HP21	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_21.mzML
SUBJECT_SAMPLE_FACTORS           	HP_22	HP22	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_22.mzML
SUBJECT_SAMPLE_FACTORS           	HP_23	HP23	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_23.mzML
SUBJECT_SAMPLE_FACTORS           	LP_4	LP4	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_4.mzML
SUBJECT_SAMPLE_FACTORS           	LP_5	LP5	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_5.mzML
SUBJECT_SAMPLE_FACTORS           	LP_6	LP6	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_6.mzML
SUBJECT_SAMPLE_FACTORS           	LP_25	LP25	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_25.mzML
SUBJECT_SAMPLE_FACTORS           	LP_26	LP26	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_26.mzML
SUBJECT_SAMPLE_FACTORS           	LP_27	LP27	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_27.mzML
SUBJECT_SAMPLE_FACTORS           	LP_28	LP28	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=LP_28.mzML
SUBJECT_SAMPLE_FACTORS           	HP_11	HP11	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_11.mzML
SUBJECT_SAMPLE_FACTORS           	HP_12	HP12	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_12.mzML
SUBJECT_SAMPLE_FACTORS           	HP_13	HP13	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_13.mzML
SUBJECT_SAMPLE_FACTORS           	HP_14	HP14	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_14.mzML
SUBJECT_SAMPLE_FACTORS           	HP_29	HP29	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_29.mzML
SUBJECT_SAMPLE_FACTORS           	HP_30	HP30	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_30.mzML
SUBJECT_SAMPLE_FACTORS           	HP_31	HP31	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_31.mzML
SUBJECT_SAMPLE_FACTORS           	HP_32	HP32	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	RAW_FILE_NAME(Raw file name)=HP_32.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_1	LAF1	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_1.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_2	LAF2	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_2.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_3	LAF3	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_3.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_4	LAF4	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_4.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_19	LAF19	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_19.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_20	LAF20	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_20.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_21	LAF21	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_21.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_22	LAF22	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_22.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_23	LAF23	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_23.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_36	LAF36	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_36.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_37	LAF37	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_37.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_38	LAF38	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_38.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_39	LAF39	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_39.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_5	W3F5	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_5.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_6	W3F6	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_6.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_7	W3F7	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_7.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_8	W3F8	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_8.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_24	W3F24	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_24.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_25	W3F25	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_25.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_26	W3F26	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_26.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_27	W3F27	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_27.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_28	W3F28	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_28.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_29	W3F29	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_29.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_40	W3F40	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_40.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_41	W3F41	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_41.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_42	W3F42	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_42.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_9	LAF9	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_9.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_10	LAF10	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_10.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_11	LAF11	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_11.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_12	LAF12	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_12.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_29	LAF29	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_29.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_30	LAF30	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_30.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_31	LAF31	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_31.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_32	LAF32	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_32.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_43	LAF43	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_43.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_44	LAF44	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_44.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_45	LAF45	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_45.mzML
SUBJECT_SAMPLE_FACTORS           	LAF_46	LAF46	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=LAF_46.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_14	W3F14	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_14.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_15	W3F15	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_15.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_16	W3F16	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_16.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_17	W3F17	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_17.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_18	W3F18	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_18.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_33	W3F33	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_33.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_34	W3F34	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_34.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_35	W3F35	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_35.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_47	W3F47	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_47.mzML
SUBJECT_SAMPLE_FACTORS           	W3F_48	W3F48	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	RAW_FILE_NAME(Raw file name)=W3F_48.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Mice were anesthetized using isofluorane. Blood was collected by intracardiac
CO:COLLECTION_SUMMARY            	puncture. EDTA was used as anticoagulant. Blood was centrifuged at 12,000xg for
CO:COLLECTION_SUMMARY            	20 mins and plasma was collected and subsequently frozen in liquid nitrogen.
CO:COLLECTION_SUMMARY            	Samples are stored at -80°C. Allow mouse to defecate normally and collect the
CO:COLLECTION_SUMMARY            	first two fecal pellets per animal into an tube. Samples are stored at -80°C.
CO:SAMPLE_TYPE                   	Feces and Plasma
#TREATMENT
TR:TREATMENT_SUMMARY             	Pla2g10+/+ and Pla2g10-/- mice fed a high fat diet, low fat diet, ω3 PUFA (fish
TR:TREATMENT_SUMMARY             	oil)-rich diet or lard-rich control diet for 24 weeks.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	After homogenization with a bead homogenizer, the samples were centrifuged at
SP:SAMPLEPREP_SUMMARY            	18,900× g for 10 min at 4 °C, and the supernatants were collected. Afterwards,
SP:SAMPLEPREP_SUMMARY            	the solutions were transferred to membrane filters (Nanosep 3K centrifugal
SP:SAMPLEPREP_SUMMARY            	device, Omega membrane; Pall Corporation) and centrifuged at 19,000× g for 20
SP:SAMPLEPREP_SUMMARY            	min to remove any floating particulates in the supernatants. For analysis of
SP:SAMPLEPREP_SUMMARY            	mouse plasma, 380 μL of water and 20 μL of the internal standard solution were
SP:SAMPLEPREP_SUMMARY            	added to 200 μL of the plasma and mixed well. For derivatization of SCFAs, 20
SP:SAMPLEPREP_SUMMARY            	μL of 20 mM 4-acetoamido-7-mercapto-2,1,3-benzoxadiazole (Tokyo Chemical
SP:SAMPLEPREP_SUMMARY            	Industry), triphenylphosphine, and 2,2’-dipyridyl disulfide (Sigma-Aldrich) in
SP:SAMPLEPREP_SUMMARY            	dichloromethane were added to the samples in a glass tube, and derivatization
SP:SAMPLEPREP_SUMMARY            	was performed at room temperature for 5 min while vortexing. The reaction
SP:SAMPLEPREP_SUMMARY            	solution was dried under vacuum, and then reconstituted with 200 μL of
SP:SAMPLEPREP_SUMMARY            	methanol.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	ExionLC
CH:COLUMN_NAME                   	Agilent Pursuit 5 C18 column
CH:SOLVENT_A                     	0.1 % formic acid in water
CH:SOLVENT_B                     	0.1% formic acid in acetonitrile
CH:FLOW_GRADIENT                 	30% B at 0 min, 30 to 50% B in 30 min, 50 to 30% B in 0.1 min, and 30% B in 4.9
CH:FLOW_GRADIENT                 	min.
CH:FLOW_RATE                     	0.5 ml/min
CH:COLUMN_TEMPERATURE            	40°C
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 6500 QTrap
MS:INSTRUMENT_TYPE               	QTRAP
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Identification was conducted using MRM transition and retention times, and
MS:MS_COMMENTS                   	quantification was performed based on the peak area of each isotope-labeled
MS:MS_COMMENTS                   	internal standard to normalize that of straight or branched SCFA having the same
MS:MS_COMMENTS                   	number of carbons. The data are reported in the following units: mmol/kg of
MS:MS_COMMENTS                   	feces, while for plasma, the unit of measurement is µM.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	µM (for plasma) or mmol/kg (for feces)
MS_METABOLITE_DATA_START
Samples	LF1	LF2	LF3	LF15	LF16	LF17	LF18	LF19	HF7	HF8	HF9	HF10	HF20	HF21	HF22	HF23	LF4	LF5	LF6	LF25	LF26	LF27	LF28	HF11	HF12	HF13	HF14	HF29	HF30	HF31	HF32	LP1	LP2	LP3	LP15	LP16	LP17	LP18	LP19	HP7	HP8	HP9	HP10	HP20	HP21	HP22	HP23	LP4	LP5	LP6	LP25	LP26	LP27	LP28	HP11	HP12	HP13	HP14	HP29	HP30	HP31	HP32	LAF1	LAF2	LAF3	LAF4	LAF19	LAF20	LAF21	LAF22	LAF23	LAF36	LAF37	LAF38	LAF39	W3F5	W3F6	W3F7	W3F8	W3F24	W3F25	W3F26	W3F27	W3F28	W3F29	W3F40	W3F41	W3F42	LAF9	LAF10	LAF11	LAF12	LAF29	LAF30	LAF31	LAF32	LAF43	LAF44	LAF45	LAF46	W3F14	W3F15	W3F16	W3F17	W3F18	W3F33	W3F34	W3F35	W3F47	W3F48
Factors	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:Low fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10-/- | Treatment:High fat diet | Sample source:Plasma	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10+/+ | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:Lard-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces	Genotype:Pla2g10-/- | Treatment:w3-rich diet | Sample source:Feces
Acetic acid	77.42123099	35.36537047	68.21996274	76.65056881	66.16364475	115.8585365	97.02150725	95.40332812	36.24953132	9.415389646	44.97029164	37.00001884	41.1652013	66.63817079	43.11990221	67.83179233	46.18361509	65.15077548	36.46121035	46.99144111	64.45656236	61.05064157	73.8346619	21.67660856	22.47465088	44.98647701	40.3799979	43.01415529	46.64849419	40.84846503	43.74879003	305.0475013	507.1814796	209.6250984	327.4608378	465.2393837	304.6570356	481.1884391	292.2224982	477.2806132	437.2356605	363.8785247	488.8509641	204.4886758	277.8541672	316.8735885	195.6033843	454.6716739	419.5349493	397.2679041	209.0122562	303.0790364	318.0185361	226.6519903	428.4591856	341.8362723	442.0508413	367.2506831	208.1135728	148.9756186	279.5460667	321.6775851	42.8552788	45.79410713	50.46779447	53.8064868	84.57849254	55.6433622	64.49303155	48.5500021	52.95664275	79.37576889	51.67227709	72.49685889	61.08574916	79.06620973	68.05864274	68.87727384	66.53900603	68.57077355	64.2141241	78.04147171	70.27017279	78.88593662	53.75361235	62.26119061	47.08520716	54.86327917	36.05589946	40.95608686	45.50506643	15.64318554	48.52802706	53.76174199	58.80205397	41.80491279	52.2887169	16.17952559	54.11571899	65.21399056	57.44808397	61.22278506	69.88883798	102.8215879	56.52703527	76.56908901	73.71024637	91.62516292	49.57157821	27.51465911
Propionic acid	27.61186015	10.51712975	21.75847195	15.0212822	14.11762766	19.28582545	12.81311432	16.73435107	3.720962324	0.266157924	3.406706823	3.459434007	4.019172821	9.60612749	6.885913245	5.177147058	6.815378141	17.34357605	5.400403212	12.40819853	14.71693927	11.71382475	14.84088554	2.108346268	1.304710947	3.455752368	2.918486851	4.202621142	4.984121645	4.64492081	5.40494006	32.11584894	64.96600109	33.10105598	45.47407895	53.36580698	46.88591913	58.03711305	39.25212926	63.99450732	54.50601477	38.47532924	58.16109399	30.99587239	40.26914179	45.78932324	29.11329779	62.17509734	57.48460237	52.72135517	27.51208373	38.64354193	41.96181928	26.026847	56.62874711	43.57401595	53.34654282	49.3332902	28.07963931	16.36621955	39.55269463	34.57317309	3.141710753	3.253277386	4.305129852	3.930876968	4.727473756	6.304673805	7.096686752	3.156315852	6.828888388	1.689099294	3.517781127	4.482315724	5.231847682	8.437120597	1.613466965	2.247957506	6.491481822	5.893518427	4.765320802	2.894391222	8.586142962	2.211129034	4.727413756	3.448367539	2.086558556	1.390484918	2.202142907	4.167394879	1.346308092	1.561966462	3.136302039	3.792045384	3.714765705	4.081277799	5.369577511	0.742381872	3.279092327	3.113460929	1.624517248	2.546151069	3.486265307	7.725432723	3.428762743	4.367208788	2.345320871	5.855911108	5.354194856	4.59859975
Butyric acid	27.01726737	9.996866551	28.02106863	10.88414474	10.09096453	15.60327825	13.61664656	13.69781982	5.059229982	1.021564555	5.369887641	3.973207042	5.086122656	9.507345989	4.625024947	7.577131728	6.68623854	14.62538607	4.800626524	13.2319885	13.23090275	9.298464906	26.90888061	2.439180803	3.753508761	6.262240538	3.765398308	3.971036489	2.544904275	3.638198411	3.384842247	10.4576265	15.07768432	11.02173471	9.330108263	9.717333253	10.01890013	11.65428869	10.33602405	13.10273711	11.27862533	8.734474402	13.18352455	8.06032395	10.04990533	10.00302016	10.91855457	13.18315206	13.47536538	14.82520939	8.440209298	9.991464878	9.751443639	8.172686929	11.83961757	11.65807137	13.20615495	9.321541379	9.490777359	7.781949342	9.405820725	8.821533834	2.629324868	2.017783097	3.656790113	2.289803239	2.99377329	3.183085949	6.751960691	4.928957355	3.358544188	1.752500645	2.996016696	4.313640357	5.721969867	4.793549405	2.742256116	3.441832142	5.515905221	2.793177768	3.050191047	2.615529239	6.357869568	2.294412104	4.28082624	2.903969312	2.194250183	3.277068455	1.34604655	3.36078111	0.791519398	1.469754994	2.148635759	3.654887568	3.82932913	4.490313109	2.723792128	1.409993301	2.114543026	2.023301848	4.412612455	2.44702951	3.177510017	4.265796755	3.406468573	2.900443029	1.821064329	2.315003296	3.12436741	4.23093312
Isobutyric acid																																																																																																														
2,2-Dimethylpropionic acid																																																																																																														
2-Methylbutyric acid																																																																																																														
Isovaleric acid																																																																																																														
Valeric acid																																																																																																														
2,2-Dimethylbutyric acid																																																																																																														
2-Ethylbutyric acid																																																																																																														
2-Methylvaleric acid																																																																																																														
Caproic acid																																																																																																														
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	quantified m/z (Q1)	quantified m/z (Q3)	PubChem ID
Acetic acid	252	210	176
Propionic acid	266	210	1032
Butyric acid	280	210	264
Isobutyric acid	280	210	6590
2,2-Dimethylpropionic acid	294	210	6417
2-Methylbutyric acid	294	210	8314
Isovaleric acid	294	210	10430
Valeric acid	294	210	7991
2,2-Dimethylbutyric acid	308	210	11684
2-Ethylbutyric acid	308	210	6915
2-Methylvaleric acid	308	210	7341
Caproic acid	308	210	8892
METABOLITES_END
#END