#METABOLOMICS WORKBENCH KasparasP_20240820_054031 DATATRACK_ID:5127 STUDY_ID:ST003419 ANALYSIS_ID:AN005618 PROJECT_ID:PR002115 VERSION 1 CREATED_ON August 20, 2024, 6:09 am #PROJECT PR:PROJECT_TITLE TRAM–LAG1–CLN8 family proteins are acyltransferases regulating phospholipid PR:PROJECT_TITLE composition PR:PROJECT_SUMMARY The diversity of cellular phospholipids, crucial for membrane homeostasis and PR:PROJECT_SUMMARY function, arises from enzymatic remodeling of their fatty acyl chains. In this PR:PROJECT_SUMMARY work, we reveal that poorly understood TRAM–LAG1–CLN8 domain-containing PR:PROJECT_SUMMARY (TLCD) proteins are phospholipid remodeling enzymes. We demonstrate that TLCD1 PR:PROJECT_SUMMARY is an evolutionarily conserved lysophosphatidylethanolamine acyltransferase, PR:PROJECT_SUMMARY which regulates cellular phospholipid composition and generates novel fatty acid PR:PROJECT_SUMMARY and thiamine (vitamin B1) esters as its secondary products. Furthermore, we PR:PROJECT_SUMMARY establish that human TLCD protein CLN8, mutations in which cause fatal PR:PROJECT_SUMMARY neurodegenerative Batten disease, is a lysophosphatidylglycerol acyltransferase. PR:PROJECT_SUMMARY We show that CLN8 catalyzes the essential step in the biosynthesis of PR:PROJECT_SUMMARY bis(monoacylglycero)phosphate, a phospholipid critical for lysosome function. PR:PROJECT_SUMMARY Our study unveils a new family of acyltransferases integral to cellular membrane PR:PROJECT_SUMMARY phospholipid homeostasis and human disease. PR:INSTITUTE University of Cambridge PR:LAST_NAME Petkevicius PR:FIRST_NAME Kasparas PR:ADDRESS The Keith Peters Building, Cambridge, Cambridgeshire, CB2 0XY, United Kingdom PR:EMAIL kp416@mrc-mbu.cam.ac.uk PR:PHONE +447500233355 #STUDY ST:STUDY_TITLE Analysis of lipid composition of the mitochondria isolated from TLCD1 KO cell ST:STUDY_TITLE models ST:STUDY_SUMMARY Untargeted lipidomics analysis was performed on whole cells and isolated ST:STUDY_SUMMARY mitochondria from control and TLCD1 knockout (KO) cell lines, both of which ST:STUDY_SUMMARY feature genetically tagged mitochondria. ST:INSTITUTE University of Cambridge ST:LAST_NAME Petkevicius ST:FIRST_NAME Kasparas ST:ADDRESS The Keith Peters Building, Cambridge, Cambridgeshire, CB2 0XY, United Kingdom ST:EMAIL kp416@mrc-mbu.cam.ac.uk ST:PHONE +447500233355 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Cell process blank Genotype:Process blank | MitoTag:Process blank | Sample source:Process blank | Replicate:Process blank RAW_FILE_NAME(Raw file name)=MitoIP process blank.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa Wild-Type pool MYC R1 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:1 RAW_FILE_NAME(Raw file name)=HeLa WholeCell WT MYC Tag 1.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa Wild-Type pool MYC R2 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:2 RAW_FILE_NAME(Raw file name)=HeLa WholeCell WT MYC Tag 2.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa Wild-Type pool MYC R3 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:3 RAW_FILE_NAME(Raw file name)=HeLa WholeCell WT MYC Tag 3.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa TLCD1 KO pool MYC R1 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:1 RAW_FILE_NAME(Raw file name)=HeLa WholeCell TLCD1 KO MYC Tag 1.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa TLCD1 KO pool MYC R2 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:2 RAW_FILE_NAME(Raw file name)=HeLa WholeCell TLCD1 KO MYC Tag 2.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa TLCD1 KO pool MYC R3 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:3 RAW_FILE_NAME(Raw file name)=HeLa WholeCell TLCD1 KO MYC Tag 3.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa Wild-Type pool HA R1 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:1 RAW_FILE_NAME(Raw file name)=HeLa WholeCell WT HA Tag 1.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa Wild-Type pool HA R2 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:2 RAW_FILE_NAME(Raw file name)=HeLa WholeCell WT HA Tag 2.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa Wild-Type pool HA R3 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:3 RAW_FILE_NAME(Raw file name)=HeLa WholeCell WT HA Tag 3.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa TLCD1 KO pool HA R1 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:1 RAW_FILE_NAME(Raw file name)=HeLa WholeCell TLCD1 KO HA Tag 1.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa TLCD1 KO pool HA R2 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:2 RAW_FILE_NAME(Raw file name)=HeLa WholeCell TLCD1 KO HA Tag 2.raw SUBJECT_SAMPLE_FACTORS - WholeCell HeLa TLCD1 KO pool HA R3 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:3 RAW_FILE_NAME(Raw file name)=HeLa WholeCell TLCD1 KO HA Tag 3.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool MYC R1 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:1 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT Myc Tag 1.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool MYC R2 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:2 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT Myc Tag 2.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool MYC R3 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:3 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT Myc Tag 3.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool MYC R4 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:4 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT Myc Tag 4.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool MYC R5 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:5 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT Myc Tag 5.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool MYC R6 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:6 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT Myc Tag 6.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool MYC R1 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:1 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO Myc Tag 1.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool MYC R2 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:2 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO Myc Tag 2.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool MYC R3 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:3 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO Myc Tag 3.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool MYC R4 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:4 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO Myc Tag 4.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool MYC R5 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:5 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO Myc Tag 5.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool MYC R6 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:6 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO Myc Tag 6.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool HA R1 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:1 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT HA Tag 1.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool HA R2 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:2 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT HA Tag 2.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool HA R3 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:3 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT HA Tag 3.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool HA R4 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:4 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT HA Tag 4.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool HA R5 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:5 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT HA Tag 5.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa Wild-Type pool HA R6 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:6 RAW_FILE_NAME(Raw file name)=HeLa MitoIP WT HA Tag 6.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool HA R1 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:1 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO HA Tag 1.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool HA R2 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:2 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO HA Tag 2.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool HA R3 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:3 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO HA Tag 3.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool HA R4 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:4 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO HA Tag 4.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool HA R5 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:5 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO HA Tag 5.raw SUBJECT_SAMPLE_FACTORS - MitoIP HeLa TLCD1 KO pool HA R6 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:6 RAW_FILE_NAME(Raw file name)=HeLa MitoIP TLCD1 KO HA Tag 6.raw #COLLECTION CO:COLLECTION_SUMMARY All the mito-IP steps were conducted using ice-cold buffers either in a CO:COLLECTION_SUMMARY cold-room or on ice and all centrifugation steps were carried out at 4oC. Cells CO:COLLECTION_SUMMARY were washed twice with 30 mL of PBS and then harvested in 1 mL of mito-IP buffer CO:COLLECTION_SUMMARY (10 mM KH2PO4, 137 mM KCl). Cells were collected at 700 x g for 5 min and CO:COLLECTION_SUMMARY resuspended in 1 mL of mito-IP buffer containing protease inhibitors (Thermo CO:COLLECTION_SUMMARY Fisher Scientific, 78429) per 15 cm plate and then lysed using 25 passes through CO:COLLECTION_SUMMARY a Dounce homogeniser. After 1,500 x g centrifugation of the lysate for 10 min, a CO:COLLECTION_SUMMARY post-nuclear supernatant (PNS) was obtained and incubated with 100 μL of CO:COLLECTION_SUMMARY anti-HA magnetic beads (Sigma-Aldrich, SAE0197), previously equilibrated in the CO:COLLECTION_SUMMARY mito-IP buffer. Finally, beads were collected using a magnetic rack, washed CO:COLLECTION_SUMMARY three times with 1 mL of mito-IP buffer for 5 min each, and dried beads were CO:COLLECTION_SUMMARY subsequently frozen in 2 mL glass autosampler vials. CO:SAMPLE_TYPE Cultured cells #TREATMENT TR:TREATMENT_SUMMARY No treatment, cells were collected under normal growth conditions and TR:TREATMENT_SUMMARY immunoprecipitation was performed as described under 'collection. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The extraction of total lipids from cellular matrices, immunoprecipitated SP:SAMPLEPREP_SUMMARY mitochondria and in vitro assays was conducted employing the butanol-methanol SP:SAMPLEPREP_SUMMARY (BUME) method, as described in detail (37). Note that the chloroform-methanol SP:SAMPLEPREP_SUMMARY lipid extraction methods result in the partitioning of acyl-thiamines into the SP:SAMPLEPREP_SUMMARY polar phase. 2 ml screw cap plastic tubes were used (3469-11, Thermo SP:SAMPLEPREP_SUMMARY Scientific), and a blank extraction was always performed in parallel to account SP:SAMPLEPREP_SUMMARY for the plastic-related contaminants. The extraction commenced with the SP:SAMPLEPREP_SUMMARY homogenization of frozen cell pellets, mitochondrial beads or in vitro assay SP:SAMPLEPREP_SUMMARY mixtures in a 0.5 ml ice-cold solution of butanol to methanol in a 3:1 ratio. SP:SAMPLEPREP_SUMMARY For lipidomics samples, BUME solution was enriched with SPLASH internal standard SP:SAMPLEPREP_SUMMARY mix (330707, Avanti Polar Lipids). For the extraction, a further 0.5 ml of 1% SP:SAMPLEPREP_SUMMARY acetic acid and 0.5 ml of a heptane:ethyl acetate 3:1 mixture were added, SP:SAMPLEPREP_SUMMARY followed by vigorous vortexing for a total of 5 minutes. The mixture was then SP:SAMPLEPREP_SUMMARY centrifuged at 6000 g for 5 minutes, allowing for the separation of phases, SP:SAMPLEPREP_SUMMARY after which the upper organic phase was carefully decanted into glass vials. A SP:SAMPLEPREP_SUMMARY second extraction was conducted on the remaining aqueous phase, with the newly SP:SAMPLEPREP_SUMMARY acquired upper phase being combined in the same glass vials as the first. Post SP:SAMPLEPREP_SUMMARY extraction, the solvents were evaporated under a stream of nitrogen, and the SP:SAMPLEPREP_SUMMARY resultant dry lipid extracts were preserved at −70°C pending further SP:SAMPLEPREP_SUMMARY analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Dried samples were reconstituted in 100 μL of isopropanol, acetonitrile, and CH:CHROMATOGRAPHY_SUMMARY water (2:1:1 ratio) and thoroughly vortexed. Liquid chromatography was conducted CH:CHROMATOGRAPHY_SUMMARY using a Shimadzu HPLC System, with 10 μL of the sample introduced onto a Waters CH:CHROMATOGRAPHY_SUMMARY Acquity Premier UPLC® CSH column (1.7 μm pore size, 2.1 mm × 50 mm), which CH:CHROMATOGRAPHY_SUMMARY was maintained at 55 °C. The mobile phase A comprised a 6:4 ratio of CH:CHROMATOGRAPHY_SUMMARY acetonitrile to water with 10 mM ammonium formate, and mobile phase B consisted CH:CHROMATOGRAPHY_SUMMARY of a 9:1 ratio of isopropanol to acetonitrile with 10 mM ammonium formate. A CH:CHROMATOGRAPHY_SUMMARY flow rate of 500 μL per minute was maintained with a gradient protocol for CH:CHROMATOGRAPHY_SUMMARY mobile phase B as follows: 0.00 minutes_40% mobile phase B; 1.5 minutes_40% CH:CHROMATOGRAPHY_SUMMARY mobile phase B; 8.00 minutes_99% mobile phase B; 10.00 minutes_99% mobile phase CH:CHROMATOGRAPHY_SUMMARY B; 10.10 minutes_40% mobile phase B; 12.00 minutes_40% mobile phase. The sample CH:CHROMATOGRAPHY_SUMMARY injection needle was rinsed with a 9:1 isopropanol and acetonitrile solution CH:CHROMATOGRAPHY_SUMMARY (strong wash) and isopropanol, acetonitrile, and water (2:1:1, weak wash). CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Shimadzu 10A CH:COLUMN_NAME Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) CH:SOLVENT_A 6:4 of acetonitrile to water with 10 mM ammonium formate CH:SOLVENT_B 9:1 of isopropanol to acetonitrile with 10 mM ammonium formate CH:FLOW_GRADIENT Gradient protocol for mobile phase B as follows: 0.00 minutes_40% mobile phase CH:FLOW_GRADIENT B; 1.5 minutes_40% mobile phase B; 8.00 minutes_99% mobile phase B; 10.00 CH:FLOW_GRADIENT minutes_99% mobile phase B; 10.10 minutes_40% mobile phase B; 12.00 minutes_40% CH:FLOW_GRADIENT mobile phase. CH:FLOW_RATE 500 μL per minute CH:COLUMN_TEMPERATURE 55 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS Mass spectrometric detection was carried out on a ThermoFisher Scientific MS:MS_COMMENTS Q-Exactive Orbitrap equipped with a heated electrospray ionization source. The MS:MS_COMMENTS mass spectrometer was calibrated immediately before sample analysis using MS:MS_COMMENTS positive and negative ionization calibration solution (recommended by Thermo MS:MS_COMMENTS Scientific). The electrospray ionization parameters were optimized with a 50:50 MS:MS_COMMENTS mix of mobile phase A and B for spray stability, setting the capillary MS:MS_COMMENTS temperature at 300 °C, source heater temperature at 420 °C, with the sheath, MS:MS_COMMENTS auxiliary, and spare gas flows at specific arbitrary units (40, 15 and 3, MS:MS_COMMENTS respectively), and source voltage at 4 kV. The mass spectrometer operated at a MS:MS_COMMENTS scan rate of 4 Hz, yielding a resolution of 35,000 at m/z 200, over a full-scan MS:MS_COMMENTS range from m/z 120 to 1800, with continuous switching between positive and MS:MS_COMMENTS negative modes. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol MS_METABOLITE_DATA_START Samples Cell process blank WholeCell HeLa Wild-Type pool MYC R1 WholeCell HeLa Wild-Type pool MYC R2 WholeCell HeLa Wild-Type pool MYC R3 WholeCell HeLa TLCD1 KO pool MYC R1 WholeCell HeLa TLCD1 KO pool MYC R2 WholeCell HeLa TLCD1 KO pool MYC R3 WholeCell HeLa Wild-Type pool HA R1 WholeCell HeLa Wild-Type pool HA R2 WholeCell HeLa Wild-Type pool HA R3 WholeCell HeLa TLCD1 KO pool HA R1 WholeCell HeLa TLCD1 KO pool HA R2 WholeCell HeLa TLCD1 KO pool HA R3 MitoIP HeLa Wild-Type pool MYC R1 MitoIP HeLa Wild-Type pool MYC R2 MitoIP HeLa Wild-Type pool MYC R3 MitoIP HeLa Wild-Type pool MYC R4 MitoIP HeLa Wild-Type pool MYC R5 MitoIP HeLa Wild-Type pool MYC R6 MitoIP HeLa TLCD1 KO pool MYC R1 MitoIP HeLa TLCD1 KO pool MYC R2 MitoIP HeLa TLCD1 KO pool MYC R3 MitoIP HeLa TLCD1 KO pool MYC R4 MitoIP HeLa TLCD1 KO pool MYC R5 MitoIP HeLa TLCD1 KO pool MYC R6 MitoIP HeLa Wild-Type pool HA R1 MitoIP HeLa Wild-Type pool HA R2 MitoIP HeLa Wild-Type pool HA R3 MitoIP HeLa Wild-Type pool HA R4 MitoIP HeLa Wild-Type pool HA R5 MitoIP HeLa Wild-Type pool HA R6 MitoIP HeLa TLCD1 KO pool HA R1 MitoIP HeLa TLCD1 KO pool HA R2 MitoIP HeLa TLCD1 KO pool HA R3 MitoIP HeLa TLCD1 KO pool HA R4 MitoIP HeLa TLCD1 KO pool HA R5 MitoIP HeLa TLCD1 KO pool HA R6 Factors Genotype:Process blank | MitoTag:Process blank | Sample source:Process blank | Replicate:Process blank Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:1 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:2 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:3 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:1 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:2 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:3 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:1 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:2 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:3 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:1 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:2 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Whole cell lipidomics | Replicate:3 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:1 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:2 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:3 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:4 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:5 Genotype:Wild-type | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:6 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:1 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:2 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:3 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:4 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:5 Genotype:TLCD1 KO | MitoTag:3xMYC-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:6 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:1 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:2 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:3 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:4 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:5 Genotype:Wild-type | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:6 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:1 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:2 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:3 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:4 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:5 Genotype:TLCD1 KO | MitoTag:3xHA-EGFP-OMP25 | Sample source:Mitochondria IP lipidomics | Replicate:6 PE_(34:1) 0 0.263347693 0.320126307 0.288306356 0.320099395 0.271864654 0.204742207 0.254260403 0.262904546 0.203714641 0.239438863 0.239429087 0.182809493 0.001788139 0.001821444 0.001505596 0.002783417 0.002391701 0.001292807 0.002332948 0.001949354 0.001533608 0.001630839 0.000867346 0.00029352 0.022550037 0.025982315 0.016984383 0.015953559 0.015541245 0.012253462 0.01233143 0.023337314 0.020121245 0.008972075 0.009724465 0.009176758 PE_(34:2) 0 0.036634873 0.034845477 0.028809806 0.013256535 0.009919956 0.012014148 0.021491951 0.033606016 0.02011551 0.003679635 0.011186718 0.008812316 0.000380196 0.000230996 0 0.000164656 0.000315991 0 0 0.00019239 0 1.60218E-06 3.92132E-06 0 0.004660483 0.005187812 0.003612547 0.001678714 0.001921818 0.001030436 0.000815809 0.001404921 0.001087349 0.00032601 0.000743074 0.000240605 PE_(35:1) 0 0.037776717 0.039934708 0.037453533 0.054788809 0.041955647 0.033072791 0.029541007 0.037647975 0.030312127 0.040847224 0.040279256 0.034779412 0.000150858 7.39645E-07 0.000164793 0.000445045 0.000394799 0.000174092 0.000132539 0.000154626 5.98315E-05 0.000428971 0.000222527 0.000206767 0.002726486 0.003241845 0.001908652 0.002901472 0.002588114 0.001959455 0.002023456 0.003467319 0.003016053 0.001805246 0.002032152 0.002163335 PE_(36:1) 0 0.440661421 0.470330014 0.501721605 0.679707703 0.551636034 0.438784674 0.393559311 0.459532584 0.386814709 0.514484603 0.531144389 0.395775024 0.001618546 0.001547584 0.001400315 0.002620002 0.001972569 0.001166522 0.002423489 0.002490727 0.001861667 0.002476271 0.00167977 0.001300871 0.020421459 0.02324479 0.016652201 0.016422074 0.016037649 0.012519452 0.014645878 0.028082973 0.02062358 0.01003076 0.010932515 0.010867927 PE_(36:2) 0 0.325543534 0.381210722 0.340797184 0.218424991 0.177419609 0.139155727 0.264126863 0.341938216 0.277706435 0.15940582 0.155850638 0.147181558 0.002538249 0.002292495 0.001875372 0.00313947 0.003226358 0.001562725 0.001494221 0.001527894 0.001294708 0.001640781 0.000928513 0.000950895 0.025361103 0.025795893 0.017381245 0.018406333 0.018241575 0.0149797 0.008563082 0.016084297 0.01150595 0.005894321 0.006211794 0.005788425 PE_(36:4) 0 0.144397096 0.170892353 0.154617508 0.211641843 0.173254606 0.140758051 0.01863744 0.156993655 0.128547604 0.15935725 0.161044477 0.130691852 0.000549521 0.00065336 0.000397948 0.001338512 0.000733367 0.000275112 0.0007439 0.001009772 0.000735398 0.00104825 0.00054333 6.81988E-05 0.008716136 0.008125714 0.006578789 0.006509241 0.005701445 0.005277343 0.006590507 0.009648951 0.007947657 0.004187676 0.005139219 0.004251552 PE_(36:5) 0 0.030526524 0.032750172 0.034405707 0.025405719 0.016660742 0.016020531 0.025438767 0.032883395 0.028882089 0.015139704 0.019948552 0.010138123 0.000131565 0.000190244 9.88447E-05 2.44308E-05 0.000137714 4.10686E-05 1.96312E-05 0 0 0 0 2.39747E-05 0.001897695 0.001926773 0.001171984 0.001160899 0.001048337 0.00085942 0.000587742 0.000967617 0.000851494 0.00032943 0.000309499 0.000283131 PE_(38:4) 0 0.535088595 0.577456609 0.592706458 0.746581257 0.655061131 0.551761183 0.45166241 0.551948241 0.454260836 0.628779221 0.61124342 0.60864618 0.001782411 0.001173649 0.001502526 0.004668263 0.003351656 0.00174706 0.002763967 0.003501189 0.001850348 0.005173144 0.00263283 0.001922876 0.034344936 0.039178547 0.025334134 0.043967362 0.037232622 0.033947865 0.031045723 0.057957939 0.042380622 0.038646324 0.03831073 0.033735039 PE_(38:5) 0 0.141991861 0.196357383 0.180520405 0.163341775 0.122833267 0.104069627 0.133347997 0.186962553 0.124212686 0.11517421 0.135767264 0.09688991 0.000798459 0.00069196 0.000565893 0.001431483 0.001045294 0.000531689 0.000902945 0.000747845 0.000373598 0.000563483 0.000261615 0.000263108 0.012777874 0.014010691 0.00839134 0.010630435 0.009506338 0.007895103 0.006320727 0.010891304 0.008732489 0.005384217 0.004967982 0.005470213 PE_(38:6) 0 0.09021487 0.105719087 0.103724057 0.121641896 0.108296607 0.085292891 0.084541237 0.099991895 0.08352864 0.099376222 0.101375231 0.074637994 4.21103E-05 0.000194109 0.000125876 0.000414732 0.000270138 0.000154615 0.000287612 0.000272189 0.000186614 6.11954E-05 0.00017502 0.000140561 0.002881443 0.003223337 0.000437116 0.002423811 0.002211143 0.00160866 0.002177165 0.003403422 0.002918247 0.001507535 0.001590631 0.001479702 PE_(40:7) 0 0.003337705 0.006104098 0.003957011 0.007381208 0.004335343 0.004983247 0.0027633 0.005457589 0.003706735 0.005502779 0.004692653 0.005500498 0.000210209 0.000113314 5.65229E-05 0.000271802 0.000271878 0.000103431 0.000177329 0.000308548 0.000143093 0.000302528 6.44027E-05 0.000130763 0.002295016 0.002367795 0.002002116 0.002773634 0.002028705 0.002258717 0.00207134 0.003512148 0.002395874 0.002401366 0.002186759 0.001985635 PC_(28:0) 0 0.078370996 0.069646469 0.075639806 0.085157843 0.075465952 0.061427862 0.066078275 0.077936125 0.062228741 0.062372759 0.060113808 0.048915423 0.000372147 0.00045404 0.00255801 0.001573818 0.000299918 0.000405938 0.002133584 0.002426578 0.000304205 0.000302279 0.000218301 0.001770691 0.000367029 0.000263244 0.00130673 0.000668008 0.000352336 0.000196433 0.001315009 0.000880773 0.000218908 0.000200898 0.001194228 0.000924648 PC_(29:0) 0 0.043773962 0.039096493 0.041288948 0.055253508 0.039332875 0.03642188 0.039079773 0.046845005 0.037477363 0.041445593 0.035932825 0.030731845 0.000140254 0.00017632 0.000789814 0.000504879 9.77386E-05 0.000121614 0.000622267 0.000761317 0.000103331 0.000104312 0.000449319 0.000570051 0.000187873 0.000161744 0.000565096 0.00040798 0.000180235 8.78847E-05 0.000602033 0.000426913 8.85965E-05 7.73168E-05 0.000514195 0.000406109 PC_(30:1) 0 0.184660023 0.164136982 0.161745923 0.224411027 0.162495992 0.139492323 0.155453668 0.178778442 0.149951956 0.14699641 0.129518966 0.117542509 0.000430361 0.000481942 0.003828783 0.002534982 0.000346502 0.000422997 0.002966715 0.003609631 0.000370647 0.000423227 0.001914233 0.002665815 0.000437857 0.00027694 0.003267109 0.000925946 0.000399566 0.000248243 0.002055595 0.002191445 0.000236699 0.00014855 0.001746892 0.001184701 PC_(31:0) 0 0.211508745 0.206337875 0.201130583 0.266775256 0.233851614 0.192783753 0.212855367 0.200399273 0.217050419 0.249200597 0.21958326 0.19704701 0.000774188 0.00078885 0.000586042 0.001435309 0.001136185 0.00054426 0.000906945 0.000826476 0.000747413 0.001180751 0.000669405 0.000580797 0.003829056 0.002656597 0.00193019 0.002885352 0.002992142 0.002427627 0.002613305 0.004099734 0.002740222 0.002671178 0.002268315 0.00225764 PC_(31:1) 0 0.141526394 0.125132251 0.132898514 0.181208594 0.131496231 0.127055138 0.130846734 0.137461776 0.138417883 0.133013101 0.131923109 0.099992367 0.000475877 0.000567236 0.003366611 0.002353404 0.000417122 0.000616583 0.002920901 0.003644525 0.000447206 0.000493646 0.001722092 0.002717184 0.000678607 0.000538665 0.002193982 0.00150179 0.000606085 0.000399437 0.002188954 0.001704332 0.000352632 0.000327314 0.001975406 0.001690461 PC_(32:0) 0 1.641290405 1.500179418 1.519914394 1.729570107 1.503637285 1.318737405 1.342055524 1.513580543 1.486208243 1.526151803 1.420820032 1.247792685 0.010289279 0.009555587 0.007359209 0.015795042 0.011186995 0.008120933 0.010980846 0.010714173 0.00852692 0.011898339 0.007256982 0.007372052 0.040659885 0.036650606 0.024491917 0.0328906 0.034038684 0.030906861 0.027163227 0.043277306 0.033779144 0.024725479 0.023397922 0.026876787 PC_(32:1) 0 2.547208133 2.402899973 2.389896983 2.941168107 2.40013217 2.201642075 2.316578033 2.497912961 2.348328503 2.335775539 2.055319111 1.780665756 0.015391368 0.019196779 0.065285319 0.075911915 0.014286233 0.017132446 0.109654105 0.114064403 0.013536788 0.014014619 0.063897161 0.09164787 0.018141101 0.012766544 0.064495595 0.037061198 0.016592664 0.008704317 0.064515423 0.043828777 0.009721403 0.008110796 0.05585661 0.044473586 PC_(32:2) 0 0.223114828 0.186629121 0.20313388 0.282272685 0.266082118 0.229503698 0.246449336 0.2315573 0.184746104 0.198263135 0.165588605 0.144811895 0.001435153 0.001078468 0.001336857 0.001081438 0.000954333 0.00059265 0.001696986 0.001563433 0.00097516 0.000681581 0.000546205 0.00049082 0.012938414 0.008841411 0.007891827 0.004146101 0.004561174 0.004371382 0.005671326 0.009050075 0.007043757 0.002435155 0.003013941 0.002939316 PC_(33:0) 0 0.109054961 0.101925073 0.102897135 0.120294362 0.096926396 0.08527621 0.098396238 0.104344882 0.094637269 0.113813517 0.087655786 0.084153624 0.000124942 0.000497302 0.000389916 0.000662902 0.000498785 0.000318854 0.000581639 0.000578874 0.000446578 0.000127132 0.000330798 0.000300104 0.002406603 0.002174415 0.000403596 0.001726909 0.001595472 0.001434473 0.001524333 0.002769939 0.002145565 0.001229838 0.001297499 0.001261998 PC_(33:1) 0 0.405176433 0.389135819 0.401986429 0.472592329 0.418310896 0.369492449 0.375783483 0.432493232 0.374364377 0.417183083 0.392307755 0.336782277 0.001535845 0.001981612 0.009527132 0.008094479 0.00131773 0.001548864 0.009482238 0.01178922 0.001156014 0.001282249 0.005325091 0.009163627 0.002327852 0.002084435 0.008578838 0.006299479 0.002075607 0.001328416 0.008034709 0.006784739 0.001156567 0.000990873 0.006771008 0.006154128 PC_(34:0) 0 0.261579519 0.219906038 0.235095211 0.265453288 0.221327761 0.201115062 0.211790226 0.263785738 0.232835208 0.25897265 0.231179602 0.197206389 0.000760798 0.000649402 0.000546211 0.001513272 0.001056787 0.000699355 0.000843303 0.000805531 0.000740488 0.001290489 0.000733204 0.00060146 0.003002874 0.002554413 0.001818592 0.003242383 0.00295368 0.002664524 0.002175866 0.00373789 0.002618981 0.002746543 0.00261267 0.002573398 PC_(34:1) 0 3.417326375 3.247244724 3.272832968 3.751823007 3.043930407 3.123869575 3.11934547 3.394003224 3.237353395 3.333730161 3.150943878 2.664980512 0.036149096 0.034060729 0.03027648 0.051340638 0.04250392 0.026979263 0.041650294 0.039777478 0.032828524 0.038347594 0.025117373 0.0257876 0.232012294 0.214535221 0.138861148 0.152618966 0.168893078 0.146203126 0.162602447 0.240004725 0.188667955 0.103733992 0.11073363 0.114157498 PC_(34:2) 0 1.29098311 1.183618229 1.18973014 1.49009448 1.219533575 1.105092948 1.143723504 1.259644048 1.139077228 1.21551357 1.073455342 0.931246209 0.001804603 0.002273365 0.006108667 0.008570246 0.00705861 0.004179884 0.008045919 0.00779611 0.00673249 0.001483865 0.003721184 0.003732347 0.061147282 0.057671036 0.010040797 0.031199321 0.010229506 0.029354959 0.035732813 0.055603905 0.041072406 0.018785349 0.022672157 0.022931732 PC_(34:3) 0 0.102091294 0.089064503 0.092277555 0.126393592 0.095420141 0.091289168 0.093480568 0.098730133 0.095574032 0.091914623 0.089511406 0.069280697 0.000515328 0.000532222 0.003351258 0.002073675 0.000472612 0.000488058 0.003140083 0.003017142 0.00042294 0.000442708 0.002052442 0.002126066 0.000516599 0.000386094 0.001669973 0.001145519 0.00051009 0.00035903 0.001783441 0.001207145 0.000279551 0.000274887 0.001538899 0.001281063 PC_(35:0) 0 0.009980505 0.008795741 0.009476796 0.011700222 0.009880146 0.00840047 0.008913214 0.009420995 0.00890685 0.010629816 0.009216009 0.007521356 1.25448E-05 2.40038E-06 1.0098E-05 3.15278E-05 1.62262E-05 0 1.55281E-05 1.95605E-05 6.27905E-06 1.66884E-05 5.45562E-06 1.38544E-05 0.00010008 7.93756E-05 5.35851E-05 7.55702E-05 6.34322E-05 5.0086E-05 6.26485E-05 0.000136062 9.35794E-05 5.23902E-05 4.78056E-05 3.93614E-05 PC_(35:1) 0 0.209389377 0.191776131 0.183209899 0.235202049 0.190361212 0.184728393 0.182569812 0.159547196 0.183556015 0.203683798 0.181448435 0.171849627 0.000286741 0.000259458 0.000266083 0.001399982 0.001218633 0.000446186 0.000473175 0.000514023 0.000405373 0.000841862 0.000644964 0.000269932 0.002354316 0.002247242 0.001207886 0.004329327 0.004283492 0.002851443 0.002288298 0.003252929 0.002323242 0.003276076 0.003696628 0.003379085 PC_(35:2) 0 0.22193236 0.202333204 0.204364923 0.246365993 0.210559739 0.188518371 0.205724201 0.215678793 0.19646527 0.216467379 0.190144786 0.175342156 0.000776224 0.001006667 0.000779515 0.001355576 0.001201703 0.000173458 0.001106312 0.000967552 0.000898268 0.001223847 0.000837957 0.000840036 0.005361285 0.004993191 0.00327754 0.00449523 0.004339066 0.003917818 0.004210442 0.006459902 0.004796664 0.003657638 0.003837655 0.003840595 PC_(36:1) 0 0.918488718 0.81882569 0.869816434 1.030148548 0.871468502 0.791367746 0.786153709 0.923797668 0.856042353 0.917866238 0.864127834 0.686710162 0.0030099 0.002849109 0.00250397 0.005662577 0.004118025 0.002631929 0.003530654 0.003731745 0.003091307 0.004630777 0.002749812 0.002406947 0.018661652 0.016693314 0.011230628 0.015785205 0.015463473 0.014654141 0.014009951 0.022947063 0.017653219 0.012115588 0.013013473 0.013414902 PC_(36:2) 0 1.462805718 1.428618694 1.484801911 1.685416475 1.345023768 1.223438863 1.391094987 1.486852935 1.375658055 1.428769698 1.220695176 1.145254865 0.01045767 0.009616635 0.009273383 0.014347355 0.01255339 0.007450139 0.012663209 0.011688776 0.009717993 0.01118004 0.00731116 0.006937357 0.088966934 0.080867203 0.015909138 0.054378682 0.05381324 0.050684177 0.05472878 0.088873219 0.066869999 0.034866247 0.040285573 0.040301656 PC_(36:3) 0 0.282774064 0.273888102 0.289431151 0.360788357 0.285267187 0.272084668 0.276632185 0.272940477 0.245619902 0.301345646 0.276728341 0.241689755 0.000868627 0.001041611 0.008444365 0.005167265 0.000725637 0.000962452 0.00670332 0.008838913 0.000757164 0.000778278 0.00436349 0.005775996 0.001172996 0.001040263 0.005204817 0.00627883 0.001102134 0.000619058 0.004934673 0.003958466 0.000499451 0.000487024 0.004095372 0.003821498 PC_(36:4) 0 0.560732072 0.535932395 0.533292788 0.578686899 0.499517209 0.469492414 0.54137746 0.529651027 0.530310884 0.53125615 0.476708687 0.405643113 0.001156188 0.001397654 0.012852784 0.008561616 0.001033376 0.001160132 0.012591625 0.012668343 0.000969924 0.001070851 0.006087418 0.009942436 0.002395341 0.001684386 0.010629889 0.007455259 0.001843058 0.000926453 0.009788446 0.007594679 0.000898853 0.00083708 0.008584396 0.007116043 PC_(36:5) 0 0.304208584 0.226015375 0.268871905 0.302696383 0.255213358 0.231285705 0.267240875 0.256899462 0.276321808 0.247569996 0.240581206 0.201036111 0.000266862 0.000401771 0.002905768 0.002608607 0.000253809 0.000365119 0.003004889 0.003880179 0.000289749 0.000266344 0.001612908 0.002736562 0.000895095 0.000584081 0.003337831 0.003066497 0.000600448 0.000318629 0.00272972 0.002566682 0.00029274 0.000249937 0.002531965 0.002375862 PC_(37:1) 0 0.030256562 0.026704516 0.027483178 0.036447334 0.029467782 0.024494105 0.027717253 0.028113338 0.026235421 0.029406256 0.025833792 0.021185696 1.80076E-05 1.90752E-05 1.22558E-05 9.27159E-05 5.85752E-05 1.62127E-05 2.78523E-05 2.41074E-05 1.88954E-05 6.64275E-05 3.28804E-05 9.85629E-06 0.000389329 0.00030956 0.000172193 0.000346665 0.000353506 0.000314453 0.000277007 0.000477668 0.000361453 0.000257182 0.0002646 0.000266927 PC_(37:2) 0 0.071584265 0.065023404 0.062410601 0.082284194 0.066961785 0.059647392 0.061095454 0.069299405 0.059688508 0.064905331 0.059567946 0.054552338 0.000164161 0.000153262 0.00013322 0.00038599 0.000301885 0.000129941 0.000218247 0.000253493 0.000164963 0.000308028 0.000145886 0.00013922 0.001775638 0.001647526 0.00097005 0.001565429 0.001524608 0.001228256 0.001275954 0.002092588 0.001382636 0.000875001 0.001178842 0.001025058 PC_(37:3) 0 0.017660087 0.014250974 0.012737893 0.01789295 0.016274157 0.013917509 0.015061212 0.015461223 0.015411915 0.016412272 0.015185902 0.012401316 1.86339E-05 2.50628E-05 1.70975E-05 2.20938E-05 2.28008E-05 6.33509E-06 2.1636E-05 2.76374E-05 1.5871E-05 3.98498E-05 1.9705E-05 1.36394E-05 0.000241386 0.000160633 0.000213152 0.00025695 0.000168318 0.000156232 0.000237872 0.000188122 0.000141229 0.000184715 0.000187522 0.000206472 PC_(37:4) 0 0.02479125 0.022306847 0.023217142 0.022952626 0.024107917 0.019833732 0.023825341 0.023423888 0.022331429 0.024675638 0.021750937 0.021715762 5.29961E-06 2.35639E-05 0.000161932 0.000158552 0 0 0.000222881 0.000277479 0 5.82718E-06 7.13679E-05 0.000212672 9.39015E-05 3.90225E-05 0.00032117 0.000343287 4.05853E-05 1.18506E-05 0.000263648 0.000265517 1.58094E-05 1.88034E-05 0.000240059 0.000215048 PC_(38:1) 0 0.028498895 0.025466399 0.028187082 0.034790611 0.026692318 0.024962344 0.024155449 0.030528087 0.025168769 0.025074879 0.026637234 0.020160142 1.46424E-05 3.2336E-05 1.45428E-05 4.37314E-05 3.25261E-05 1.44077E-05 4.93287E-05 3.35815E-05 1.70521E-05 5.10232E-05 1.38431E-05 0 0.000409348 0.000352036 0.000262991 0.000301147 0.000367298 0.000300061 0.000270948 0.000469169 0.000398658 0.000235485 0.000259926 0.000165391 PC_(38:2) 0 0.184722284 0.159146417 0.171049938 0.215437433 0.174894382 0.157646582 0.147665314 0.172721194 0.153748453 0.160706341 0.150882132 0.117440827 0.00068955 0.00064842 0.000611157 0.000901119 0.000720512 0.00047011 0.00078543 0.000796359 0.000649324 0.000644304 0.000420926 0.000397362 0.005347368 0.005045788 0.003360022 0.002927245 0.003075463 0.002850218 0.003265635 0.005547699 0.004139633 0.001705726 0.002089614 0.002210703 PC_(38:3) 0 0.104550088 0.077407912 0.098814257 0.098114911 0.096073052 0.086032124 0.098197251 0.099573664 0.099244162 0.094914598 0.083147061 0.077761558 0.00029087 0.000281633 0.0002725 0.00053465 0.000403376 0.000206364 0.000391313 0.000362359 0.000291333 0.000313659 0.000228731 0.000205854 0.003252873 0.00324957 0.001988207 0.001977519 0.002135883 0.00204771 0.001975139 0.003113491 0.002341022 0.001578569 0.001548965 0.001404811 PC_(38:4) 0 0.237821418 0.231896959 0.250147591 0.251155563 0.212954005 0.188304532 0.239291416 0.24752214 0.243276406 0.265246397 0.22040112 0.215530917 0.000420712 0.000326703 0.000422111 0.001497978 0.000959894 0.000516448 0.000543328 0.00049629 0.000430155 0.001315008 0.000619979 0.000505212 0.003433443 0.003286365 0.002071362 0.005705601 0.004637863 0.004390122 0.002989568 0.004940172 0.003275404 0.0054673 0.004754624 0.003934155 PC_(38:5) 0 0.088546043 0.093467499 0.097918704 0.093361729 0.090861364 0.084462612 0.095120863 0.103968599 0.088850837 0.09846689 0.10134519 0.083206052 3.1748E-06 5.558E-05 0.000768706 0.000739985 1.59575E-05 4.97199E-05 0.000848289 0.00116859 3.74517E-05 1.50393E-05 0.00035766 0.001161 0.000412824 0.000304782 0.001548923 0.001615785 0.000145176 4.06434E-05 0.001144205 0.001245923 4.92904E-07 1.85143E-05 0.001115533 0.000971776 PC_(40:2) 0 0.021301724 0.019771429 0.021497762 0.027112314 0.021125935 0.01935605 0.018157949 0.022327746 0.018842618 0.018623662 0.01925593 0.014305455 0.000106502 0.000142062 2.06687E-05 6.85471E-05 4.97851E-05 6.52256E-05 0.00011437 8.25674E-05 3.29467E-05 6.98641E-05 5.23597E-05 1.16842E-05 0.00056512 0.00052281 0.000326455 0.000309861 0.000385825 0.000340263 0.000390341 0.000625457 0.000457227 0.000244922 0.000241228 0.000221609 PC_(40:4) 0 0.042326643 0.032687681 0.065246642 0.053100769 0.036080073 0.061680843 0.027039022 0.039037262 0.058518786 0.069332211 0.060595158 0.053280539 3.90192E-05 5.18719E-05 6.56599E-05 0.000178506 0.000290017 3.26473E-05 0.000104559 7.85633E-05 4.17653E-05 0.000290011 4.4921E-05 7.53901E-05 0.000496065 0.000589751 0.000234526 0.000502428 0.000720658 0.000375092 0.000480592 0.000823616 0.000559367 0.000970241 0.000590614 0.000572182 PC_(40:6) 0 0.091911803 0.08481479 0.083251472 0.101589135 0.088331748 0.074096196 0.07845406 0.091809467 0.085118544 0.0940409 0.085357118 0.07613324 0.000121122 0.000104891 9.81717E-05 0.000530311 0.000258843 0.000130917 0.000149652 0.000154728 0.000127137 0.000417504 0.00015958 0.000165811 0.001022803 0.001075419 0.000568043 0.001697116 0.0014733 0.001434766 0.000957102 0.001440807 0.001067356 0.001817448 0.001556888 0.001426068 PC_(40:7) 0 0.002495385 0.000556039 0.001955166 0.001830142 0.002304952 0.002291787 0.002741063 0.001948961 0.002414844 0.00186405 0.001950241 0.002607278 1.92916E-07 0 0 0 0 0 9.44456E-08 1.0012E-07 0 8.03424E-06 0 0 0.000217339 0.000145605 0.000181475 9.39845E-05 0.00017618 7.24733E-05 6.02343E-05 0.00021247 0.000101869 7.4559E-05 4.68477E-05 8.36132E-05 CL_(66:03) 0 0.000664433 0.000688892 0.001072201 0.000775138 0.00072569 0.000738861 0.00066663 0.000820799 0.000673668 0.00072882 0.000872119 0.000630394 0 0 0 0 0 0 0 0 0 0 0 0 0.000401422 0.000548193 0.000389743 0.000261364 0.000263803 0.000230324 0.000377246 0.000536651 0.000423247 9.48804E-05 0.000126301 0.000184781 CL_(66:04) 0 0.000551156 0.000633262 0.000776885 0.000751251 0.000553361 0.000467903 0.000471025 0.000708939 0.000557151 0.000594467 0.00045289 0.000409509 0 0 0 0 0 0 0 0 0 0 0 0 0.000505052 0.000533814 0.000360809 0.000224281 0.00021328 0.000175589 0.000273152 0.000417046 0.000310497 9.13748E-05 8.44365E-05 0.000138237 CL_(68:02) 0 0.000983913 0.001240968 0.001530535 0.001433475 0.001044898 0.001073309 0.001105763 0.001447573 0.001344316 0.001313441 0.001310631 0.001222024 0 0 0 0 0 0 0 0 0 0 0 0 0.000378065 0.00049327 0.000271155 0.000398352 0.000332953 0.000270067 0.000332753 0.000474619 0.000359457 0.00022593 0.000272979 0.000199499 CL_(68:03) 0 0.001118508 0.001304208 0.001638259 0.001355136 0.001344545 0.001277719 0.001123481 0.001539746 0.001227486 0.001317901 0.001342791 0.001254268 0 8.07631E-06 0 0 0 0 0 0 0 6.79133E-06 0 0 0.000605883 0.000856944 0.000460677 0.000489907 0.000464627 0.000345291 0.000507929 0.000684843 0.000611436 0.000332924 0.000321832 0.000359289 CL_(68:04) 0 0.003372774 0.003633793 0.005158291 0.004261874 0.00377662 0.003557478 0.003203127 0.004526912 0.00342521 0.00368941 0.004210811 0.003297294 3.57119E-05 4.16915E-05 3.74872E-05 3.01895E-05 7.13974E-05 0 5.09846E-05 8.23959E-05 4.55078E-05 3.06533E-05 3.36105E-05 8.9053E-06 0.002071554 0.002785201 0.001853202 0.001405558 0.001293641 0.001088641 0.001624952 0.002513063 0.002005601 0.000540515 0.000795596 0.000855975 CL_(68:05) 0 0.001187466 0.001395842 0.001613436 0.001725167 0.001348573 0.001149149 0.001096217 0.001622843 0.001307495 0.001421794 0.001353698 0.000991189 0 0 0 3.03117E-05 1.59477E-05 0 0 6.32056E-06 0 8.07272E-06 6.72853E-06 0 0.000672423 0.000789415 0.000509538 0.000513566 0.00043356 0.00039246 0.000513909 0.000631475 0.000559468 0.000209778 0.000271663 0.000359589 CL_(69:04) 0 0.000728452 0.000760871 0.001054019 0.001101925 0.00099035 0.00090752 0.000751105 0.000998574 0.000914051 0.000879672 0.000987988 0.000798794 0 0 0 0 0 0 0 0 0 0 0 0 0.000364208 0.000439555 0.000195724 0.000243994 0.00025366 0.00017709 0.000331539 0.000404074 0.000342795 0.000121153 0.000198332 0.000160468 CL_(69:05) 0 0.000359539 0.000450055 0.000703582 0.000641904 0.000512196 0.000475029 0.000390806 0.000578603 0.00042276 0.000688918 0.000701755 0.000502077 0 0 0 0 0 0 0 0 0 0 0 0 0.000151116 0.000158435 7.64813E-05 0.000113552 6.41286E-05 8.71386E-05 0.000134594 0.000216518 0.000162562 6.16904E-05 7.77816E-05 0.000105913 CL_(70:03) 0 0.000736867 0.00081826 0.001065501 0.001149724 0.000813357 0.00084461 0.000764527 0.001149127 0.000984232 0.000892733 0.000988639 0.000929534 0 0 0 0 0 0 0 0 0 0 0 0 0.000314349 0.000376511 0.000228358 0.000254214 0.000266811 0.000177321 0.000248884 0.000340553 0.000290682 0.000177143 0.000182655 0.000180472 CL_(70:04) 0 0.003132316 0.00349918 0.004193804 0.00370908 0.003559242 0.003459486 0.00289429 0.004495857 0.003317239 0.003496931 0.003519755 0.003277659 3.55696E-05 3.24208E-05 4.46882E-05 7.28105E-05 7.55223E-05 9.61003E-06 5.29662E-05 5.05303E-05 9.15841E-06 3.54009E-05 1.99627E-05 1.22721E-05 0.00161804 0.002140336 0.00117011 0.001364652 0.001250589 0.001006917 0.001465296 0.001928749 0.001632878 0.000875786 0.000860769 0.001002521 CL_(70:07) 0 0.000745986 0.000663235 0.001008854 0.000833081 0.000846289 0.000722409 0.00073658 0.000915299 0.000735845 0.000778785 0.000811428 0.000661791 0 0 0 0 0 0 0 0 0 0 0 0 0.000333693 0.000513989 0.000274351 0.000200839 0.000197846 0.000186398 0.000333599 0.000471374 0.000326936 6.90707E-05 9.03967E-05 0.000104868 CL_(71:04) 0 0.000334059 0.000417931 0.000576868 0.000578062 0.000493493 0.000451893 0.000342052 0.000626299 0.000433933 0.000620021 0.000531874 0.000409538 0 0 0 0 0 0 0 0 0 0 0 0 7.90169E-05 0.000166841 3.3339E-05 0.000114887 5.79839E-05 9.1828E-05 6.12122E-05 0.000117691 0.000178909 7.70333E-05 0.000105723 7.56776E-05 CL_(71:05) 0 0.000699795 0.000727839 0.001012225 0.001026518 0.000926794 0.000831341 0.000744254 0.000994672 0.000861476 0.000970824 0.001084502 0.000923759 0 0 0 0 0 0 0 0 0 0 0 0 0.000189427 0.000246732 0.000115553 0.000220214 0.000216695 0.000155432 0.000255642 0.000259773 0.00023682 0.000156794 0.000204121 0.000193497 CL_(72:04) 0 0.000950367 0.00107628 0.001382778 0.001494662 0.001056504 0.000980305 0.001049707 0.00134598 0.001148661 0.001313236 0.0012848 0.001145294 0 0 0 0 0 0 0 0 0 0 0 0 0.000408735 0.000515084 0.000303314 0.000320525 0.000318994 0.000245789 0.000399484 0.000537918 0.000435251 0.000155935 0.000284199 0.00022512 CL_(72:05) 0 0.002194087 0.002499272 0.0030535 0.003050374 0.002672103 0.002704002 0.002212531 0.003535963 0.002498586 0.003073595 0.002976168 0.002744064 0 0 7.21581E-06 5.58942E-05 3.96136E-05 0 0 1.53142E-05 0 2.74955E-05 0 0 0.000861282 0.00108898 0.00057273 0.000964121 0.00088722 0.000719077 0.000886893 0.001223702 0.000919254 0.000673953 0.000723343 0.00087477 CL_(72:07) 0 0.000759519 0.000818008 0.00103753 0.000982985 0.000902944 0.000894882 0.000749143 0.001136538 0.000794104 0.000887912 0.000830973 0.000753074 0 0 0 0 0 0 0 0 0 0 0 0 0.000316149 0.000371243 0.000217249 0.000168283 0.000210873 0.000132745 0.000251189 0.000379121 0.000273353 0.000117017 9.34409E-05 0.000134442 CL_(72:08) 0 0.001219775 0.001190938 0.001462685 0.001426285 0.001255517 0.001225752 0.001086409 0.001527547 0.001312743 0.001328279 0.001461372 0.001233071 0 0 0 0 0 0 0 0 0 0 0 0 0.000349633 0.000461676 0.000275204 0.000294238 0.00027801 0.000210376 0.000332832 0.000521474 0.000362812 0.000123325 0.000197975 0.000174967 CL_(72:09) 0 0.000338698 0.000350797 0.000476644 0.000431765 0.000417862 0.000309543 0.000300159 0.000477472 0.000392771 0.000400919 0.000402939 0.000322642 0 0 0 0 0 0 0 0 0 0 0 0 8.51299E-05 8.17579E-05 2.95165E-05 5.70508E-05 2.79265E-05 5.29032E-05 4.56267E-05 0.000105406 7.92826E-05 4.7543E-05 4.8947E-05 5.31905E-05 CL_(74:08) 0 0.00052081 0.000622567 0.000734414 0.000730726 0.000690247 0.000687163 0.000578628 0.000834627 0.000677446 0.00068191 0.000720764 0.000689979 0 0 0 0 0 0 0 0 0 0 0 0 0.000137872 0.000212063 7.64792E-05 0.000117869 0.000133951 7.99843E-05 0.000147229 0.000272176 0.000181672 5.41276E-05 9.45184E-05 8.10747E-05 CL_(74:09) 0 0.000711856 0.000746642 0.000838468 0.000939262 0.000761825 0.000748107 0.000610517 0.000958209 0.000780104 0.000843439 0.000969902 0.000840205 0 0 0 0 0 0 0 0 0 0 0 0 0.000143922 0.000166082 7.65719E-05 0.000165409 0.000103351 9.45943E-05 9.90383E-05 0.000173156 0.000119624 9.17957E-05 0.000160873 8.12616E-05 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name m/z [M+H]+ Average Rt(min) PE_(34:1) 718.53748 4.718 PE_(34:2) 716.51257 4.226 PE_(35:1) 732.54883 4.96 PE_(36:1) 746.57025 5.179 PE_(36:2) 744.54633 4.748 PE_(36:4) 740.52222 4.184 PE_(36:5) 738.50525 3.74 PE_(38:4) 768.55365 4.741 PE_(38:5) 766.53766 4.265 PE_(38:6) 764.52191 4.066 PE_(40:7) 790.53632 4.748 PC_(28:0) 678.50745 3.086 PC_(29:0) 692.52307 3.476 PC_(30:1) 704.52277 3.192 PC_(31:0) 720.55438 4.2 PC_(31:1) 718.53833 3.623 PC_(32:0) 734.56995 4.517 PC_(32:1) 732.55438 3.982 PC_(32:2) 730.53888 3.445 PC_(33:0) 748.58588 4.704 PC_(33:1) 746.57068 4.292 PC_(34:0) 762.6015 5.015 PC_(34:1) 760.58539 4.581 PC_(34:2) 758.56989 4.135 PC_(34:3) 756.55414 3.623 PC_(35:0) 776.617 5.156 PC_(35:1) 774.6004 4.839 PC_(35:2) 772.58563 4.389 PC_(36:1) 788.61737 5.063 PC_(36:2) 786.60101 4.653 PC_(36:3) 784.58502 4.238 PC_(36:4) 782.56921 4.026 PC_(36:5) 780.55432 3.627 PC_(37:1) 802.63214 5.244 PC_(37:2) 800.61615 4.873 PC_(37:3) 798.60083 4.49 PC_(37:4) 796.58496 4.339 PC_(38:1) 816.64148 5.457 PC_(38:2) 814.6311 5.087 PC_(38:3) 812.61639 4.733 PC_(38:4) 810.6001 4.621 PC_(38:5) 808.58472 4.299 PC_(40:2) 842.66296 5.482 PC_(40:4) 838.63239 4.981 PC_(40:6) 834.60059 4.517 PC_(40:7) 832.58246 4.42 CL_(66:03) 1373.94629 6.526 CL_(66:04) 1371.93323 6.363 CL_(68:02) 1403.995 6.861 CL_(68:03) 1401.97913 6.704 CL_(68:04) 1399.96179 6.54 CL_(68:05) 1397.94861 6.395 CL_(69:04) 1413.98193 6.63 CL_(69:05) 1411.9624 6.488 CL_(70:03) 1430.01099 6.866 CL_(70:04) 1427.99573 6.717 CL_(70:07) 1421.94458 6.541 CL_(71:04) 1442.01807 6.793 CL_(71:05) 1439.99561 6.651 CL_(72:04) 1456.02759 6.88 CL_(72:05) 1454.01086 6.737 CL_(72:07) 1449.97754 6.718 CL_(72:08) 1447.96167 6.569 CL_(72:09) 1445.94592 6.424 CL_(74:08) 1475.99304 6.737 CL_(74:09) 1473.97534 6.594 METABOLITES_END #END