#METABOLOMICS WORKBENCH coopergwen823_20240829_091219 DATATRACK_ID:5154 STUDY_ID:ST003474 ANALYSIS_ID:AN005709 PROJECT_ID:PR002133
VERSION             	1
CREATED_ON             	September 11, 2024, 8:25 pm
#PROJECT
PR:PROJECT_TITLE                 	Impact of Cattle Feeding Systems on Beef and Human Post-prandial Metabolomics
PR:PROJECT_TITLE                 	– A Randomized Clinical Trial
PR:PROJECT_SUMMARY               	Cattle feeding systems may have health implications for consumers of beef
PR:PROJECT_SUMMARY               	products. Organic grass-fed (GRA) and conventional (CON) cattle feeding systems
PR:PROJECT_SUMMARY               	may result in beef products with differing metabolite profiles, and therefore
PR:PROJECT_SUMMARY               	could impact the postprandial metabolomic response of consumers. This study aims
PR:PROJECT_SUMMARY               	to measure whole beef metabolomics and postprandial metabolomic response of
PR:PROJECT_SUMMARY               	consumers between GRA and CON beef to elucidate potential health implications.
PR:PROJECT_SUMMARY               	This study followed a double-blind, crossover design with healthy male and
PR:PROJECT_SUMMARY               	female subjects (n=10). Serum samples were taken at fasting (0) and
PR:PROJECT_SUMMARY               	postprandially for four hours after consumption of a steak from each condition.
PR:PROJECT_SUMMARY               	Untargeted metabolomic analysis of whole beef and human serum samples utilized
PR:PROJECT_SUMMARY               	LC/MS. Multivariate and pathway enrichment analysis in MetaboAnalyst was used to
PR:PROJECT_SUMMARY               	investigate metabolite and biochemical pathways that distinguished CON and GRA.
PR:PROJECT_SUMMARY               	Cattle feeding systems impacted both postprandial and whole beef steak
PR:PROJECT_SUMMARY               	metabolomic profiles. Metabolites that contributed to this variation included
PR:PROJECT_SUMMARY               	carnitine species (Proionylcarnitine), fatty acids, amino acids (L-Valine) and
PR:PROJECT_SUMMARY               	Calamendiol. These metabolites have been associated with oxidative stress,
PR:PROJECT_SUMMARY               	inflammation, and cardiovascular health. Functional pathway enrichment analysis
PR:PROJECT_SUMMARY               	revealed numerous amino acid degradation pathways, especially branched chain
PR:PROJECT_SUMMARY               	amino acids, and fatty acid degradation that changed throughout the postprandial
PR:PROJECT_SUMMARY               	time course. These findings suggest that CON and GRA cattle feeding systems
PR:PROJECT_SUMMARY               	differentially impact whole beef metabolomics, as well as consumer postprandial
PR:PROJECT_SUMMARY               	metabolic responses and the associated health implications.
PR:INSTITUTE                     	Montana State University
PR:LAST_NAME                     	Cooper
PR:FIRST_NAME                    	Gwendolyn
PR:ADDRESS                       	Culbertson Hall 100, Bozeman, Montana, 59717, USA
PR:EMAIL                         	coopergwen823@gmail.com
PR:PHONE                         	9079037662
#STUDY
ST:STUDY_TITLE                   	Impact of Cattle Feeding Systems on Beef and Human Postprandial Metabolomics –
ST:STUDY_TITLE                   	A Randomized Clinical Trial
ST:STUDY_SUMMARY                 	Cattle feeding systems may have health implications for consumers of beef
ST:STUDY_SUMMARY                 	products. Organic grass-fed (GRA) and conventional (CON) cattle feeding systems
ST:STUDY_SUMMARY                 	may result in beef products with differing metabolite profiles, and therefore
ST:STUDY_SUMMARY                 	could impact the postprandial metabolomic response of consumers. This study aims
ST:STUDY_SUMMARY                 	to measure whole beef metabolomics and postprandial metabolomic response of
ST:STUDY_SUMMARY                 	consumers between GRA and CON beef to elucidate potential health implications.
ST:STUDY_SUMMARY                 	This study followed a double-blind, crossover design with healthy male and
ST:STUDY_SUMMARY                 	female subjects (n=10). Serum samples were taken at fasting (0) and
ST:STUDY_SUMMARY                 	postprandially for four hours after consumption of a steak from each condition.
ST:STUDY_SUMMARY                 	Untargeted metabolomic analysis of whole beef and human serum samples utilized
ST:STUDY_SUMMARY                 	LC/MS. Multivariate and pathway enrichment analysis in MetaboAnalyst was used to
ST:STUDY_SUMMARY                 	investigate metabolite and biochemical pathways that distinguished CON and GRA.
ST:STUDY_SUMMARY                 	Cattle feeding systems impacted both postprandial and whole beef steak
ST:STUDY_SUMMARY                 	metabolomic profiles. Metabolites that contributed to this variation included
ST:STUDY_SUMMARY                 	carnitine species (Proionylcarnitine), fatty acids, amino acids (L-Valine) and
ST:STUDY_SUMMARY                 	Calamendiol. These metabolites have been associated with oxidative stress,
ST:STUDY_SUMMARY                 	inflammation, and cardiovascular health. Functional pathway enrichment analysis
ST:STUDY_SUMMARY                 	revealed numerous amino acid degradation pathways, especially branched chain
ST:STUDY_SUMMARY                 	amino acids, and fatty acid degradation that changed throughout the postprandial
ST:STUDY_SUMMARY                 	time course. These findings suggest that CON and GRA cattle feeding systems
ST:STUDY_SUMMARY                 	differentially impact whole beef metabolomics, as well as consumer postprandial
ST:STUDY_SUMMARY                 	metabolic responses and the associated health implications.
ST:INSTITUTE                     	Montana State University
ST:LAST_NAME                     	Cooper
ST:FIRST_NAME                    	GWendolyn
ST:ADDRESS                       	Culbertson HAll, 100
ST:EMAIL                         	coopergwen823@gmail.com
ST:PHONE                         	9079037662
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	Batch1_POOL	Batch1_POOL_DDA_with	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__51_Batch1_POOL_DDA_with.mzML
SUBJECT_SAMPLE_FACTORS           	Batch1_POOL	Batch1_POOL_DDA_without	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__52_Batch1_POOL_DDA_without.mzML
SUBJECT_SAMPLE_FACTORS           	Batch1_POOL	Batch1_POOL_MSE	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__53_Batch1_POOL_MSE.mzML
SUBJECT_SAMPLE_FACTORS           	Batch2_POOL	Batch2_POOL_DDA_with	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__104_Batch2_POOL_DDA_with.mzML
SUBJECT_SAMPLE_FACTORS           	Batch2_POOL	Batch2_POOL_DDA_without	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__105_Batch2_POOL_DDA_without.mzML
SUBJECT_SAMPLE_FACTORS           	Batch2_POOL	Batch2_POOL_MSE	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__106_Batch2_POOL_MSE.mzML
SUBJECT_SAMPLE_FACTORS           	Batch3_POOL	Batch3_POOL_DDA_with	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__158_Batch3_POOL_DDA_with.mzML
SUBJECT_SAMPLE_FACTORS           	Batch3_POOL	Batch3_POOL_DDA_without	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__159_Batch3_POOL_DDA_without.mzML
SUBJECT_SAMPLE_FACTORS           	Batch3_POOL	Batch3_POOL_MSE	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__160_Batch3_POOL_MSE.mzML
SUBJECT_SAMPLE_FACTORS           	Batch4_POOL	Batch4_POOL_DDA_with	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__211_Batch4_POOL_DDA_with.mzML
SUBJECT_SAMPLE_FACTORS           	Batch4_POOL	Batch4_POOL_DDA_without	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__212_Batch4_POOL_DDA_without.mzML
SUBJECT_SAMPLE_FACTORS           	Batch4_POOL	Batch4_POOL_MSE	Condition:GRA+CON | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__213_Batch4_POOL_MSE.mzML
SUBJECT_SAMPLE_FACTORS           	Blank0	Blank0	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__01_Blank0.mzML
SUBJECT_SAMPLE_FACTORS           	blank1	blank1	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__12_blank1.mzML
SUBJECT_SAMPLE_FACTORS           	blank10	blank10	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__98_blank10.mzML
SUBJECT_SAMPLE_FACTORS           	blank11	blank11	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__107_blank11.mzML
SUBJECT_SAMPLE_FACTORS           	blank12	blank12	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__119_blank12.mzML
SUBJECT_SAMPLE_FACTORS           	blank13	blank13	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__130_blank13.mzML
SUBJECT_SAMPLE_FACTORS           	blank14	blank14	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__141_blank14.mzML
SUBJECT_SAMPLE_FACTORS           	blank15	blank15	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__152_blank15.mzML
SUBJECT_SAMPLE_FACTORS           	blank16	blank16	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__161_blank16.mzML
SUBJECT_SAMPLE_FACTORS           	blank17	blank17	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__172_blank17.mzML
SUBJECT_SAMPLE_FACTORS           	blank18	blank18	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__183_blank18.mzML
SUBJECT_SAMPLE_FACTORS           	blank19	blank19	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__194_blank19.mzML
SUBJECT_SAMPLE_FACTORS           	blank20	blank20	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__205_blank20.mzML
SUBJECT_SAMPLE_FACTORS           	blank21	blank21	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__214_blank21.mzML
SUBJECT_SAMPLE_FACTORS           	blank3	blank3	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__23_blank3.mzML
SUBJECT_SAMPLE_FACTORS           	blank4	blank4	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__34_blank4.mzML
SUBJECT_SAMPLE_FACTORS           	blank5	blank5	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__45_blank5.mzML
SUBJECT_SAMPLE_FACTORS           	blank6	blank6	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__54_blank6.mzML
SUBJECT_SAMPLE_FACTORS           	blank7	blank7	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__65_blank7.mzML
SUBJECT_SAMPLE_FACTORS           	blank8	blank8	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__76_blank8.mzML
SUBJECT_SAMPLE_FACTORS           	blank9	blank9	Condition:NA | Time point:NA | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__87_blank9.mzML
SUBJECT_SAMPLE_FACTORS           	M10A_0	M10A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__133_M10A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M10A_0.5	M10A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__116_M10A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M10A_1	M10A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__115_M10A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M10A_1.5	M10A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__129_M10A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M10A_2	M10A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__124_M10A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M10A_2.5	M10A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__135_M10A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M10A_3	M10A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__138_M10A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M10A_3.5	M10A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__118_M10A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M10A_4	M10A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__157_M10A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M10B_0	M10B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__58_M10B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M10B_0.5	M10B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__82_M10B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M10B_1	M10B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__90_M10B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M10B_1.5	M10B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__94_M10B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M10B_2	M10B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__62_M10B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M10B_2.5	M10B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__57_M10B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M10B_3	M10B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__92_M10B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M10B_3.5	M10B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__91_M10B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M10B_4	M10B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__66_M10B_4.mzML
SUBJECT_SAMPLE_FACTORS           	M11A_0	M11A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__174_M11A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M11A_0.5	M11A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__209_M11A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M11A_1	M11A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__181_M11A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M11A_1.5	M11A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__202_M11A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M11A_2	M11A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__182_M11A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M11A_2.5	M11A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__179_M11A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M11A_3	M11A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__195_M11A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M11A_3.5	M11A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__208_M11A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M11A_4	M11A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__210_M11A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M11B_0	M11B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__22_M11B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M11B_0.5	M11B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__06_M11B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M11B_1	M11B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__07_M11B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M11B_1.5	M11B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__15_M11B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M11B_2	M11B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__02_M11B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M11B_2.5	M11B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__11_M11B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M11B_3	M11B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__20_M11B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M11B_3.5	M11B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__36_M11B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M11B_4	M11B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__43_M11B_4.mzML
SUBJECT_SAMPLE_FACTORS           	M13A_0	M13A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__128_M13A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M13A_0.5	M13A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__156_M13A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M13A_1	M13A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__111_M13A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M13A_1.5	M13A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__154_M13A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M13A_2	M13A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__123_M13A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M13A_2.5	M13A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__114_M13A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M13A_3	M13A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__142_M13A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M13A_3.5	M13A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__153_M13A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M13A_4	M13A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__151_M13A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M13B_0	M13B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__32_M13B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M13B_0.5	M13B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__08_M13B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M13B_1	M13B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__25_M13B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M13B_1.5	M13B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__41_M13B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M13B_2	M13B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__33_M13B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M13B_2.5	M13B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__47_M13B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M13B_3	M13B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__29_M13B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M13B_3.5	M13B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__48_M13B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M13B_4	M13B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__39_M13B_4.mzML
SUBJECT_SAMPLE_FACTORS           	M14A_0	M14A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__149_M14A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M14A_0.5	M14A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__148_M14A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M14A_1	M14A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__121_M14A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M14A_1.5	M14A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__145_M14A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M14A_2	M14A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__144_M14A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M14A_2.5	M14A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__143_M14A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M14A_3	M14A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__126_M14A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M14A_3.5	M14A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__117_M14A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M14A_4	M14A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__140_M14A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M14B_0	M14B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__13_M14B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M14B_0.5	M14B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__09_M14B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M14B_1	M14B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__49_M14B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M14B_1.5	M14B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__18_M14B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M14B_2	M14B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__05_M14B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M14B_2.5	M14B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__19_M14B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M14B_3	M14B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__26_M14B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M14B_3.5	M14B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__35_M14B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M14B_4	M14B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__50_M14B_4.mzML
SUBJECT_SAMPLE_FACTORS           	M15A_0	M15A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__79_M15A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M15A_0.5	M15A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__84_M15A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M15A_1	M15A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__56_M15A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M15A_1.5	M15A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__61_M15A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M15A_2	M15A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__70_M15A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M15A_2.5	M15A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__78_M15A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M15A_3	M15A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__95_M15A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M15A_3.5	M15A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__74_M15A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M15A_4	M15A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__97_M15A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M15B_0	M15B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__99_M15B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M15B_0.5	M15B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__68_M15B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M15B_1	M15B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__103_M15B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M15B_1.5	M15B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__80_M15B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M15B_2	M15B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__75_M15B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M15B_2.5	M15B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__71_M15B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M15B_3	M15B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__96_M15B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M15B_3.5	M15B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__81_M15B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M15B_4	M15B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__69_M15B_4.mzML
SUBJECT_SAMPLE_FACTORS           	M1A_0	M1A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__175_M1A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M1A_0.5	M1A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__178_M1A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M1A_1	M1A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__189_M1A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M1A_1.5	M1A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__173_M1A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M1A_2	M1A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__190_M1A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M1A_2.5	M1A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__180_M1A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M1A_3	M1A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__162_M1A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M1A_3.5	M1A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__169_M1A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M1A_4	M1A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__167_M1A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M1B_0	M1B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__176_M1B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M1B_0.5	M1B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__187_M1B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M1B_1	M1B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__191_M1B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M1B_1.5	M1B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__163_M1B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M1B_2	M1B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__192_M1B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M1B_2.5	M1B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__166_M1B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M1B_3	M1B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__197_M1B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M1B_3.5	M1B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__170_M1B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M1B_4	M1B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__177_M1B_4.mzML
SUBJECT_SAMPLE_FACTORS           	M4A_0	M4A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__134_M4A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M4A_0.5	M4A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__108_M4A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M4A_1	M4A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__127_M4A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M4A_1.5	M4A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__112_M4A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M4A_2	M4A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__113_M4A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M4A_2.5	M4A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__150_M4A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M4A_3	M4A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__155_M4A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M4A_3.5	M4A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__147_M4A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M4A_4	M4A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__146_M4A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M4B_0	M4B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__73_M4B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M4B_0.5	M4B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__85_M4B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M4B_1	M4B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__100_M4B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M4B_1.5	M4B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__67_M4B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M4B_2	M4B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__64_M4B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M4B_2.5	M4B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__72_M4B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M4B_3	M4B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__59_M4B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M4B_3.5	M4B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__88_M4B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M4B_4	M4B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__55_M4B_4.mzML
SUBJECT_SAMPLE_FACTORS           	M5A_0	M5A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__198_M5A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M5A_0.5	M5A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__171_M5A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M5A_1	M5A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__200_M5A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M5A_1.5	M5A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__201_M5A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M5A_2	M5A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__203_M5A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M5A_2.5	M5A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__204_M5A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M5A_3	M5A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__188_M5A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M5A_3.5	M5A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__168_M5A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M5A_4	M5A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__164_M5A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M5B_0	M5B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__139_M5B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M5B_0.5	M5B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__136_M5B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M5B_1	M5B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__122_M5B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M5B_1.5	M5B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__131_M5B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M5B_2	M5B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__120_M5B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M5B_2.5	M5B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__125_M5B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M5B_3	M5B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__132_M5B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M5B_3.5	M5B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__137_M5B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M5B_4	M5B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__109_M5B_4.mzML
SUBJECT_SAMPLE_FACTORS           	M8A_0	M8A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__83_M8A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M8A_0.5	M8A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__60_M8A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M8A_1	M8A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__86_M8A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M8A_1.5	M8A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__63_M8A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M8A_2	M8A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__89_M8A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M8A_2.5	M8A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__101_M8A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M8A_3	M8A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__102_M8A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M8A_3.5	M8A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__93_M8A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M8A_4	M8A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__77_M8A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M8B_0	M8B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__04_M8B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M8B_0.5	M8B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__03_M8B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M8B_1	M8B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__27_M8B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M8B_1.5	M8B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__31_M8B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M8B_2	M8B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__10_M8B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M8B_2.5	M8B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__38_M8B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M8B_3	M8B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__42_M8B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M8B_3.5	M8B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__28_M8B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M8B_4	M8B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__16_M8B_4.mzML
SUBJECT_SAMPLE_FACTORS           	M9A_0	M9A_0	Condition:GRA | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__46_M9A_0.mzML
SUBJECT_SAMPLE_FACTORS           	M9A_0.5	M9A_0.5	Condition:GRA | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__44_M9A_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M9A_1	M9A_1	Condition:GRA | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__17_M9A_1.mzML
SUBJECT_SAMPLE_FACTORS           	M9A_1.5	M9A_1.5	Condition:GRA | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__40_M9A_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M9A_2	M9A_2	Condition:GRA | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__24_M9A_2.mzML
SUBJECT_SAMPLE_FACTORS           	M9A_2.5	M9A_2.5	Condition:GRA | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__37_M9A_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M9A_3	M9A_3	Condition:GRA | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__21_M9A_3.mzML
SUBJECT_SAMPLE_FACTORS           	M9A_3.5	M9A_3.5	Condition:GRA | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__14_M9A_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M9A_4	M9A_4	Condition:GRA | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__30_M9A_4.mzML
SUBJECT_SAMPLE_FACTORS           	M9B_0	M9B_0	Condition:CON | Time point:0 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__199_M9B_0.mzML
SUBJECT_SAMPLE_FACTORS           	M9B_0.5	M9B_0.5	Condition:CON | Time point:0.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__193_M9B_0.5.mzML
SUBJECT_SAMPLE_FACTORS           	M9B_1	M9B_1	Condition:CON | Time point:1 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__185_M9B_1.mzML
SUBJECT_SAMPLE_FACTORS           	M9B_1.5	M9B_1.5	Condition:CON | Time point:1.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__196_M9B_1.5.mzML
SUBJECT_SAMPLE_FACTORS           	M9B_2	M9B_2	Condition:CON | Time point:2 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__206_M9B_2.mzML
SUBJECT_SAMPLE_FACTORS           	M9B_2.5	M9B_2.5	Condition:CON | Time point:2.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__165_M9B_2.5.mzML
SUBJECT_SAMPLE_FACTORS           	M9B_3	M9B_3	Condition:CON | Time point:3 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__184_M9B_3.mzML
SUBJECT_SAMPLE_FACTORS           	M9B_3.5	M9B_3.5	Condition:CON | Time point:3.5 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__186_M9B_3.5.mzML
SUBJECT_SAMPLE_FACTORS           	M9B_4	M9B_4	Condition:CON | Time point:4 | Sample source:serum	RAW_FILE_NAME(Raw_File_Name)=20231120_GC__207_M9B_4.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	During visit 1 of the study, the informed consent was reviewed verbally
CO:COLLECTION_SUMMARY            	in-person and informed consent was obtained from all participants prior to their
CO:COLLECTION_SUMMARY            	participation. All participants within the study consumed both a grass-fed and
CO:COLLECTION_SUMMARY            	grain-fed steak either on visit 2 or visit 3 as this is a crossover study.
CO:COLLECTION_SUMMARY            	Participants were instructed not to consume any red meat beginning 72 hours
CO:COLLECTION_SUMMARY            	before visit 2 through the completion of visit 3, and to avoid fatty fish the
CO:COLLECTION_SUMMARY            	day before visit 2 and 3. The diet the day before visit 2 was then replicated by
CO:COLLECTION_SUMMARY            	participants before visit 3 to improve comparability. Prior to visits 2 and 3
CO:COLLECTION_SUMMARY            	participants fasted for 12 hours and avoided strenuous activity or the
CO:COLLECTION_SUMMARY            	consumption of alcohol for 24 hours. An intravenous catheter was inserted in the
CO:COLLECTION_SUMMARY            	antecubital vein upon arrival by a phlebotomist or medical doctor. Blood samples
CO:COLLECTION_SUMMARY            	were collected into plasma separating vacutainer tubes containing heparin (BD
CO:COLLECTION_SUMMARY            	Vacutainer, Frankline Lakes, NJ, USA) after a 3 mL waste withdrawal followed by
CO:COLLECTION_SUMMARY            	a sterile saline flush. The fasting blood sample was taken 15 minutes after
CO:COLLECTION_SUMMARY            	catheter insertion. Postprandial blood samples were collected every 30 minutes
CO:COLLECTION_SUMMARY            	for 4 hours beginning 30 minutes after participant began eating the study steak.
CO:COLLECTION_SUMMARY            	Ad libitum black tea was offered to participants to accompany the steak. Blood
CO:COLLECTION_SUMMARY            	markers glucose (GLU), and triglycerides (TG) were measured at fasting and
CO:COLLECTION_SUMMARY            	hourly timepoints using whole blood collected in a sodium heparin tubes on a
CO:COLLECTION_SUMMARY            	Picollo Xpress Chemistry Analyzer lipid panel (Abaxis, Union City, CA, USA).
CO:COLLECTION_SUMMARY            	Samples were allowed to clot for 15 min prior to centrifugation (1200RPM, 15
CO:COLLECTION_SUMMARY            	minutes) to separate serum. The plasma was then aliquoted and frozen at -80°C
CO:COLLECTION_SUMMARY            	for future analysis.
CO:SAMPLE_TYPE                   	Serum
#TREATMENT
TR:TREATMENT_SUMMARY             	Participants were instructed not to consume any red meat beginning 72 hours
TR:TREATMENT_SUMMARY             	before visit 2 through the completion of visit 3, and to avoid fatty fish the
TR:TREATMENT_SUMMARY             	day before visit 2 and 3. The diet the day before visit 2 was then replicated by
TR:TREATMENT_SUMMARY             	participants before visit 3 to improve comparability. Prior to visits 2 and 3
TR:TREATMENT_SUMMARY             	participants fasted for 12 hours and avoided strenuous activity or the
TR:TREATMENT_SUMMARY             	consumption of alcohol for 24 hours. Organic, grass-fed beef strip loin was
TR:TREATMENT_SUMMARY             	sourced from B Bar Ranch Big Timber, MT and conventionally fed beef choice strip
TR:TREATMENT_SUMMARY             	loin was sourced from a local purveyor. All steaks were cut from the longissimus
TR:TREATMENT_SUMMARY             	lumborum muscle and matched for marbling. Two cows per condition were used to
TR:TREATMENT_SUMMARY             	source strip loin cuts. Steaks were vacuum sealed and stored at -20ºC until
TR:TREATMENT_SUMMARY             	needed for participants. All steaks were removed from the freezer and thawed in
TR:TREATMENT_SUMMARY             	a refrigerator between 24-48 hours before the planned time of consumption. An
TR:TREATMENT_SUMMARY             	uncooked sample of beef weighing between 2-5 grams was collected for future
TR:TREATMENT_SUMMARY             	metabolic analysis. Pre-cooking weight of the raw steak was recorded. Steaks
TR:TREATMENT_SUMMARY             	were cooked on a clamshell grill with a thermometer (ThermoWorks THS-313-158
TR:TREATMENT_SUMMARY             	probe with Therma Waterproof THS-232-101, American Fork, UT, USA) inserted into
TR:TREATMENT_SUMMARY             	the thickest part of the meat until the thermometer read 70ºC. A cooked sample
TR:TREATMENT_SUMMARY             	of beef weighing 2-5 grams was collected for future metabolic analysis. Weight
TR:TREATMENT_SUMMARY             	was recorded immediately after taking off the grill. The steak was then wrapped
TR:TREATMENT_SUMMARY             	in foil and rested for 5 minutes. After 5 minutes of rest, the cooked steak was
TR:TREATMENT_SUMMARY             	cut to 170.010 grams and served to participants.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	To extract serum metabolites and precipitate proteins, 100uL of serum was added
SP:SAMPLEPREP_SUMMARY            	to 400uL of cold acetone. Samples were then placed in the -80°C overnight.
SP:SAMPLEPREP_SUMMARY            	Proteins and any other remaining macromolecules were then pelleted via
SP:SAMPLEPREP_SUMMARY            	centrifugation at 16,100 x g for 15 minutes at 4°C. Supernatants containing
SP:SAMPLEPREP_SUMMARY            	metabolites were then collected and dried down via vacuum concentration. Samples
SP:SAMPLEPREP_SUMMARY            	were extracted in batches (1-4). Pooled samples from each batch were analyzed
SP:SAMPLEPREP_SUMMARY            	via data dependent acquisition (DDA with), data independent acquisition (DDA
SP:SAMPLEPREP_SUMMARY            	without) and MSE (tandem MS) to improve identifications of metabolites. All
SP:SAMPLEPREP_SUMMARY            	samples were stored at -80°C until subsequent mass spectrometry analysis.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Waters Acquity I-Class
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH HILIC (150 x 2.1mm,1.7um)
CH:SOLVENT_A                     	95% water/5% acetonitrile; 0.1% formic acid
CH:SOLVENT_B                     	5% acetonitrile/95% water; 0.1% formic acid
CH:FLOW_GRADIENT                 	94% B for 2min; 94% B until 8.10min; 50% B until 9.0min; 94% B until 12min, Hold
CH:FLOW_GRADIENT                 	94% B until 15min
CH:FLOW_RATE                     	0.400mL/min
CH:COLUMN_TEMPERATURE            	50
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Waters Synapt G2 XS QTOF
MS:INSTRUMENT_TYPE               	QTOF
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Progenesis QI software (Nonlinear Dynamics Newcastle, UK) was used for data
MS:MS_COMMENTS                   	processing and annotation.
MS:MS_RESULTS_FILE               	ST003474_AN005709_Results.txt	UNITS:area	Has m/z:Yes	Has RT:Yes	RT units:Minutes
#END