#METABOLOMICS WORKBENCH bagchipuja_20240812_225938 DATATRACK_ID:5099 STUDY_ID:ST003480 ANALYSIS_ID:AN005715 PROJECT_ID:PR002137
VERSION             	1
CREATED_ON             	September 18, 2024, 10:40 pm
#PROJECT
PR:PROJECT_TITLE                 	Acid-sensing receptor, GPR65, on tumor macrophages drives accelerated tumor
PR:PROJECT_TITLE                 	growth in obesity
PR:PROJECT_SUMMARY               	Multiple cancers, including colorectal cancer (CRC), are more frequent and often
PR:PROJECT_SUMMARY               	more aggressive in obese individuals. Here, we show that macrophages accumulate
PR:PROJECT_SUMMARY               	within tumors of obese CRC patients and in obese CRC mice and promote
PR:PROJECT_SUMMARY               	accelerated tumor growth. These changes are initiated by oleic acid accumulation
PR:PROJECT_SUMMARY               	and subsequent tumor cell-derived acid production, and driven by signaling
PR:PROJECT_SUMMARY               	through GPR65, an acid-sensing receptor on CRC-associated macrophages. We
PR:PROJECT_SUMMARY               	demonstrate a similar role for GPR65 in hepatocellular carcinoma (HCC) in obese
PR:PROJECT_SUMMARY               	mice. Tumors in obese patients with CRC or HCC also exhibit increased GPR65
PR:PROJECT_SUMMARY               	expression, suggesting that the mechanism revealed here likely contributes to
PR:PROJECT_SUMMARY               	tumor growth in a range of obesity-associated cancers and represents a potential
PR:PROJECT_SUMMARY               	therapeutic target.
PR:INSTITUTE                     	Stanford University
PR:LAST_NAME                     	Bagchi
PR:FIRST_NAME                    	Sreya
PR:ADDRESS                       	3373 Hill Avenue, Palo Alto, CA, 94304, USA
PR:EMAIL                         	bagchipuja@gmail.com
PR:PHONE                         	4086214773
#STUDY
ST:STUDY_TITLE                   	Fatty acid analysis of human colorectal adenocarcinoma tumors of normal weight
ST:STUDY_TITLE                   	(BMI<25) and obese (BMI>30) patients revealed an increase in oleic acid in obese
ST:STUDY_TITLE                   	patients.
ST:STUDY_SUMMARY                 	Frozen human colorectal adenocarcinoma tumors samples were acquired and analyzed
ST:STUDY_SUMMARY                 	for fatty acid composition via GC-FID. These data revealed an increase in
ST:STUDY_SUMMARY                 	several fatty acids, such as palmitic acid, stearic acid, oleic acid, etc., in
ST:STUDY_SUMMARY                 	obese patients. However, oleic acid was the most abundant fatty acid in tumors
ST:STUDY_SUMMARY                 	of obese patients and also the fatty acid that exhibited the most significant
ST:STUDY_SUMMARY                 	increase in obese patients compared to normal-weight patients.
ST:INSTITUTE                     	Stanford University
ST:LAST_NAME                     	Bagchi
ST:FIRST_NAME                    	Sreya
ST:ADDRESS                       	3373 Hill Avenue, Palo Alto, CA, 94304, USA
ST:EMAIL                         	bagchipuja@gmail.com
ST:PHONE                         	4086214773
#SUBJECT
SU:SUBJECT_TYPE                  	Human
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	Sample 1 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 2 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 3 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 4 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 5 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 6 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 7 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 8 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 9 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 10 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 11 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 12 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 13 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 14 - Normal	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 15 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 16 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 17 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 18 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 19 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 20 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 21 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 22 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 23 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 24 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 25 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 26 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 27 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 28 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
SUBJECT_SAMPLE_FACTORS           	-	Sample 29 - Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	
#COLLECTION
CO:COLLECTION_SUMMARY            	CRC tumor sample from patients were collected and frozen by the Stanford tissue
CO:COLLECTION_SUMMARY            	bank. Samples were collected from non-obese and obese patients.
CO:SAMPLE_TYPE                   	Colorectal adenocarcinoma tumors
#TREATMENT
TR:TREATMENT_SUMMARY             	Patients were treatment naive.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Tumors were surgically removed and frozen until further use.
SP:SAMPLEPREP_PROTOCOL_ID        	CRC_fatty_acid_protocol.pdf
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	GC-FID was performed on an Agilent 6890N GC coupled to a FID ( flame ionization
CH:CHROMATOGRAPHY_SUMMARY        	detector). The FID detector was operated at 260 °C. The hydrogen flow to the
CH:CHROMATOGRAPHY_SUMMARY        	detector was 30 mL/min and air flow was 400 mL/min. The sampling rate of the FID
CH:CHROMATOGRAPHY_SUMMARY        	was 20 Hz.
CH:CHROMATOGRAPHY_TYPE           	GC
CH:INSTRUMENT_NAME               	Agilent 6890N
CH:COLUMN_NAME                   	Agilent DB-23 (60m x 0.25mm x 0.25um)
CH:SOLVENT_A                     	3 M methanolic hydrochloric acid
CH:SOLVENT_B                     	100% hexane
CH:FLOW_GRADIENT                 	Temperature-programmed GC
CH:FLOW_RATE                     	1.5 mL/min
CH:COLUMN_TEMPERATURE            	250
CH:METHODS_FILENAME              	CRC_fatty_acid_protocol.pdf
CH:INTERNAL_STANDARD             	pentadecanoic acid (15:0)
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	N/A (FID detection was used)
MS:INSTRUMENT_TYPE               	Other
MS:MS_TYPE                       	Other
MS:ION_MODE                      	UNSPECIFIED
MS:MS_COMMENTS                   	No MS involved (FID detection was used)
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	nmol/mg dry wt
MS_METABOLITE_DATA_START
Samples	Sample 1 - Normal	Sample 2 - Normal	Sample 3 - Normal	Sample 4 - Normal	Sample 5 - Normal	Sample 6 - Normal	Sample 7 - Normal	Sample 8 - Normal	Sample 9 - Normal	Sample 10 - Normal	Sample 11 - Normal	Sample 12 - Normal	Sample 13 - Normal	Sample 14 - Normal	Sample 15 - Obese	Sample 16 - Obese	Sample 17 - Obese	Sample 18 - Obese	Sample 19 - Obese	Sample 20 - Obese	Sample 21 - Obese	Sample 22 - Obese	Sample 23 - Obese	Sample 24 - Obese	Sample 25 - Obese	Sample 26 - Obese	Sample 27 - Obese	Sample 28 - Obese	Sample 29 - Obese
Factors	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Non-obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese	Sample source:Colorectal adenocarcinoma tumor | Group:Obese
myristic acid	16.944	9.750	15.145	18.280	3.881	14.593	18.816	31.323	8.477	13.375	15.581	18.953	5.957	8.685	13.676	8.144	5.682	7.700	6.393	10.827	7.788	4.265	19.270	15.860	24.662	11.261	17.881	10.281	8.976
palmitic acid	365.662	319.330	324.263	228.573	340.427	315.395	337.385	409.512	283.925	323.529	321.163	293.689	406.500	460.680	395.792	393.768	428.581	371.151	443.405	383.604	390.751	468.949	487.871	423.311	341.362	322.892	379.983	387.709	395.130
palmitoleic acid	39.297	51.550	28.187	48.502	40.724	22.540	50.732	70.409	23.384	64.710	45.884	67.560	17.714	20.062	25.534	64.425	25.169	21.821	33.469	44.312	19.499	22.438	74.840	53.059	98.606	37.312	56.450	35.046	21.063
stearic acid	165.591	182.317	163.783	109.992	134.357	213.407	207.369	172.854	243.053	231.112	194.313	181.025	310.725	311.728	305.015	376.495	323.169	264.848	323.973	260.645	317.689	321.387	297.098	306.973	45.148	213.676	236.010	283.584	227.611
oleic acid	369.197	329.167	348.677	425.489	402.044	336.153	411.275	389.079	364.240	419.584	424.436	349.540	468.880	323.920	488.220	687.090	598.346	494.062	492.844	498.901	432.246	664.564	594.323	546.817	619.539	396.839	515.283	476.927	484.634
linoleic acid	327.215	144.892	226.927	114.041	245.133	204.441	230.352	187.232	297.032	207.150	193.924	160.717	442.076	278.906	350.350	416.118	420.347	280.252	334.709	353.834	386.409	463.911	291.159	261.954	234.865	304.427	281.032	412.584	337.833
alpha-linolenic acid	8.883	5.502	14.282	3.677	7.514	6.114	6.573	6.392	5.952	9.984	10.076	5.794	9.212	8.641	8.572	6.964	18.926	12.157	8.680	18.654	13.415	13.024	6.072	10.643	12.845	10.658	7.831	12.858	11.167
eicosenoic acid	3.212	5.702	5.635	6.928	6.881	8.510	12.806	5.610	8.532	8.317	16.524	11.808	20.166	6.159	9.213	12.714	9.331	16.895	10.027	16.113	12.887	4.883	16.680	9.845	5.924	13.677	14.374	10.245	5.460
icosadienoic acid	9.241	7.358	6.746	3.144	9.570	9.462	12.857	12.665	10.835	9.726	12.681	10.287	26.043	13.251	12.039	22.901	15.981	18.780	19.329	13.568	23.837	8.959	18.213	17.894	4.129	17.995	15.899	15.930	9.178
dihomo-gamma-linolenic acid	19.309	19.042	26.312	12.956	39.232	50.872	25.954	62.422	31.201	22.504	18.306	16.283	38.418	45.153	30.032	66.143	74.602	28.503	57.744	60.227	58.741	41.145	40.745	41.845	7.944	39.215	37.906	36.374	32.454
arachidonic acid	102.991	184.326	159.737	63.405	93.098	125.431	121.242	95.461	153.730	134.734	136.362	88.622	238.093	236.331	203.877	213.154	213.927	246.483	266.203	174.565	305.363	217.349	244.608	183.969	24.320	167.710	180.170	175.123	174.557
behenic acid	12.517	9.870	8.132	4.359	4.636	8.889	6.661	13.126	8.812	11.229	8.478	9.016	16.388	11.199	8.607	6.114	26.010	9.269	11.901	10.022	18.675	16.297	4.755	8.556	0.937	7.845	7.004	10.961	10.266
tetracosanoic acid	2.213	5.134	5.548	3.514	3.636	6.281	6.387	6.616	8.649	7.244	7.552	4.778	11.629	8.129	9.171	6.862	11.254	6.519	9.085	7.412	5.611	17.029	9.136	8.592	1.583	7.770	7.863	7.390	8.084
nervonic acid	1.848	5.428	5.426	3.924	3.012	5.262	7.460	6.022	5.628	5.095	9.642	4.407	16.606	25.306	10.738	16.515	9.701	8.725	27.377	5.648	10.993	8.268	14.054	9.635	3.250	13.207	9.567	7.198	9.126
docosahexaenoic acid	54.005	29.524	32.456	20.930	11.081	32.094	30.650	32.638	25.596	43.186	30.886	28.023	67.185	25.002	39.133	31.061	73.441	35.134	45.862	23.386	29.870	64.442	36.965	22.255	5.427	22.544	36.926	31.525	27.475
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	RT	PubChem ID	KEGG ID
myristic acid	7.544	11005	C06424
palmitic acid	9.637	985	C00249
palmitoleic acid	10.079	445638	C08362
stearic acid	12.151	5281	C01530
oleic acid	12.571	445639	C00712
linoleic acid	13.315	5280450	C01595
alpha-linolenic acid	14.238	5280934	C06427
eicosenoic acid	15.364	46234793	C16526
icosadienoic acid	16.171	6439848	C16525
dihomo-gamma-linolenic acid	16.684	5280581	C03242
arachidonic acid	17.028	444899	C00219
behenic acid	17.901	8215	C08281
tetracosanoic acid	21.848	11197	C08320
nervonic acid	22.723	5281120	C08323
docosahexaenoic acid	23.005	45934466	C06429
METABOLITES_END
#END