#METABOLOMICS WORKBENCH JiamengSun99_20240924_123714 DATATRACK_ID:5220 STUDY_ID:ST003495 ANALYSIS_ID:AN005736 PROJECT_ID:PR002144 VERSION 1 CREATED_ON September 25, 2024, 6:23 am #PROJECT PR:PROJECT_TITLE Hepatocyte Period 1 dictates oxidative substrate selection independent of the PR:PROJECT_TITLE core circadian clock PR:PROJECT_SUMMARY This project contains metabolomic data generated in the paper titled "Hepatocyte PR:PROJECT_SUMMARY Period 1 dictates oxidative substrate selection independent of the core PR:PROJECT_SUMMARY circadian clock" PR:INSTITUTE Washington University in St. Louis PR:LAST_NAME Sun PR:FIRST_NAME Jiameng PR:ADDRESS 660 S. Euclid Ave PR:EMAIL jiameng@wustl.edu PR:PHONE 3149349954 #STUDY ST:STUDY_TITLE Hepatocyte Period 1 dictates oxidative substrate selection independent of the ST:STUDY_TITLE core circadian clock ST:STUDY_SUMMARY We treated Per1fl/fl or Per1LKO mice with or without FGF21, fasted or refed ST:INSTITUTE Washington University in St. Louis ST:LAST_NAME Sun ST:FIRST_NAME Jiameng ST:ADDRESS 660 S. Euclid Ave ST:EMAIL jiameng@wustl.edu ST:PHONE 3149349954 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - DBLiver_pP_1A Treatment:WT_Fast | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_1A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_1B Treatment:WT_Fast | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_1B.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_2A Treatment:WT_Fast | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_2A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_4A Treatment:LKO_Fast | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_4A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_5A Treatment:LKO_Fast | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_5A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_5B Treatment:LKO_Fast | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_5B.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_3A Treatment:WT_Fast_FGF21 | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_3A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_3B Treatment:WT_Fast_FGF21 | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_3B.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_3C Treatment:WT_Fast_FGF21 | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_3C.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_6C Treatment:LKO_Fast_FGF21 | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_6C.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_6D Treatment:LKO_Fast_FGF21 | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_6D.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_6E Treatment:LKO_Fast_FGF21 | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_6E.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_2B Treatment:WT_Fast_Refeed | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_2B.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_2C Treatment:WT_Fast_Refeed | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_2C.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_2D Treatment:WT_Fast_Refeed | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_2D.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_6A Treatment:LKO_Fast_Refeed | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_6A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_pP_6B Treatment:LKO_Fast_Refeed | Sample source:Mouse liver phase=positive; RAW_FILE_NAME(Raw file name)=DBLiver_pP_6B.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_1A Treatment:WT_Fast | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_1A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_1B Treatment:WT_Fast | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_1B.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_2A Treatment:WT_Fast | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_2A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_4A Treatment:LKO_Fast | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_4A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_5A Treatment:LKO_Fast | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_5A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_5B Treatment:LKO_Fast | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_5B.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_3A Treatment:WT_Fast_FGF21 | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_3A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_3B Treatment:WT_Fast_FGF21 | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_3B.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_3C Treatment:WT_Fast_FGF21 | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_3C.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_6C Treatment:LKO_Fast_FGF21 | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_6C.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_6D Treatment:LKO_Fast_FGF21 | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_6D.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_6E Treatment:LKO_Fast_FGF21 | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_6E.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_2B Treatment:WT_Fast_Refeed | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_2B.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_2C Treatment:WT_Fast_Refeed | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_2C.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_2D Treatment:WT_Fast_Refeed | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_2D.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_6A Treatment:LKO_Fast_Refeed | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_6A.mzML SUBJECT_SAMPLE_FACTORS - DBLiver_nP_6B Treatment:LKO_Fast_Refeed | Sample source:Mouse liver phase=negative; RAW_FILE_NAME(Raw file name)=DBLiver_nP_6B.mzML #COLLECTION CO:COLLECTION_SUMMARY All mouse studies were approved by the Institutional Care and Use Committee at CO:COLLECTION_SUMMARY Washington University in Saint Louis. To perform infusion studies, a catheter CO:COLLECTION_SUMMARY (Instech, C20PU-MJV1301) was placed in the right jugular vein and connected to a CO:COLLECTION_SUMMARY vascular access button (Instech, VABM1B/25) implanted subcutaneously in the back CO:COLLECTION_SUMMARY of the mice. All surgeries were performed at the Hope Center for Neurological CO:COLLECTION_SUMMARY Diseases, Washington University. Mice were allowed to recover from surgery for CO:COLLECTION_SUMMARY at least one week before tracer infusion. U13C-Glucose (CIL, CLM-1396-PK) was CO:COLLECTION_SUMMARY fresh prepared in saline at a concentration of 200mM. The mice were weighed to CO:COLLECTION_SUMMARY calculate the tracer infusion rate. To begin infusion, the vascular access CO:COLLECTION_SUMMARY button of individual mice was connected to the infusion line with a swivel CO:COLLECTION_SUMMARY (Instech, SMCLA), tether (Instech, KVABM1T/25), and infusion pump (CHEMYX, CO:COLLECTION_SUMMARY Fusion 100T). The infusion line was prefilled with 200mM U13C-Glucose. Prime CO:COLLECTION_SUMMARY infusion was initiated at 1ul/min/g for 2 minutes to clear the catheter locking CO:COLLECTION_SUMMARY solution, followed by continued infusion at 0.1ul/min/g for 2 hours. Following CO:COLLECTION_SUMMARY completion of the glucose infusion, mice were anesthetized and blood was CO:COLLECTION_SUMMARY collected by cardiac puncture. Tissues were subsequently collected as quickly as CO:COLLECTION_SUMMARY possible (in 10 min or less) following euthanasia and snap frozen in liquid CO:COLLECTION_SUMMARY nitrogen. Tissues were stored at −80 °C until processing for LC/MS analysis. CO:SAMPLE_TYPE Liver #TREATMENT TR:TREATMENT_SUMMARY Mice are treated with or without FGF21, mice were either 16h-fasted or TR:TREATMENT_SUMMARY 14h-fasted plus 2h-refed #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The liver tissue was mixed with ice-cold methanol:acetonitrile:water (2:2:1), SP:SAMPLEPREP_SUMMARY and subjected to two cycles of 7m/s (30 s/cycle) using an Omni Bead Ruptor Elute SP:SAMPLEPREP_SUMMARY Homogenizer. For every 1 mg of tissue wet weight, 40 L of extraction solvent SP:SAMPLEPREP_SUMMARY was added. Samples were then incubated at -20 C for 1 h to precipitate SP:SAMPLEPREP_SUMMARY protein. Tissue extracts were centrifuged at 20,000 g and 4 C for 10 min, and SP:SAMPLEPREP_SUMMARY the supernatant was transferred into LC/MS vials. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Thermo Scientific QExactive Plus Mass Spectrometer CH:COLUMN_NAME HILICON iHILIC-(P)-Classic column CH:SOLVENT_A 20 mM ammonium bicarbonate, 2.5 M medronic acid, 0.1% ammonium hydroxide in CH:SOLVENT_A water:acetonitrile 95:5 CH:SOLVENT_B water:acetonitrile 5:95 CH:FLOW_GRADIENT linear gradient CH:FLOW_RATE 0.25 mL min-1: 0 – 1min, 90% B; 12min, 35% B; 12.5 – 14.5min, 25% B; 15min, CH:FLOW_RATE 90% B CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE Other MS:ION_MODE NEGATIVE MS:MS_COMMENTS LC/MS data were processed and analyzed with the open-source Skyline software MS:MS_COMMENTS [Please cite: 10.1021/acs.jproteome.9b00640] Natural-abundance correction of 13C MS:MS_COMMENTS was performed with AccuCor [Please cite: 10.1021/acs.analchem.7b00396]. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Raw Area MS_METABOLITE_DATA_START Samples DBLiver_nP_1A DBLiver_nP_1B DBLiver_nP_2A DBLiver_nP_4A DBLiver_nP_5A DBLiver_nP_5B DBLiver_nP_3A DBLiver_nP_3B DBLiver_nP_3C DBLiver_nP_6C DBLiver_nP_6D DBLiver_nP_6E DBLiver_nP_2B DBLiver_nP_2C DBLiver_nP_2D DBLiver_nP_6A DBLiver_nP_6B Factors Treatment:WT_Fast | Sample source:Mouse liver Treatment:WT_Fast | Sample source:Mouse liver Treatment:WT_Fast | Sample source:Mouse liver Treatment:LKO_Fast | Sample source:Mouse liver Treatment:LKO_Fast | Sample source:Mouse liver Treatment:LKO_Fast | Sample source:Mouse liver Treatment:WT_Fast_FGF21 | Sample source:Mouse liver Treatment:WT_Fast_FGF21 | Sample source:Mouse liver Treatment:WT_Fast_FGF21 | Sample source:Mouse liver Treatment:LKO_Fast_FGF21 | Sample source:Mouse liver Treatment:LKO_Fast_FGF21 | Sample source:Mouse liver Treatment:LKO_Fast_FGF21 | Sample source:Mouse liver Treatment:WT_Fast_Refeed | Sample source:Mouse liver Treatment:WT_Fast_Refeed | Sample source:Mouse liver Treatment:WT_Fast_Refeed | Sample source:Mouse liver Treatment:LKO_Fast_Refeed | Sample source:Mouse liver Treatment:LKO_Fast_Refeed | Sample source:Mouse liver Glucose 480156800 882604928 499437696 125763656 293439328 485249152 678298752 223717440 163012528 133915584 159170640 374747040 1395069440 758731328 2395122432 521610816 498379872 Glucose-6-phosphate 43197532 64558536 23987820 2586056 16285684 33237972 43083460 3992633 3388081 3137489 2889526 12840053 42202428 61310848 274516320 28321278 50239020 2-PG 12374171 21356494 6991031 7964541 6085206 6305328 20715944 3960325 3455312 5526133 6124147 4181681 19532216 25861020 34636208 8686202 7582216 Phosphoenolpyruvate 866342 791281 643324 539494 64193 45430 589308 3109 549157 543435 532061 16457 700669 775258 465119 607412 686933 Pyruvate 18866746 19151740 18832384 13439330 13128332 11897089 16212395 11663147 11850837 12418637 17297960 13334051 14460763 16539466 35076656 14823320 14777251 Lactate 9621530624 16057648128 12883002368 10296256512 5899410432 8729619456 16923632640 5113273344 4451938304 10232492032 11137055744 7193425408 41485320192 39288979456 44350894080 15849614336 12110410752 Ribose-5-phosphate 58113688 77858008 49686776 32878508 22795652 45343520 50863772 22255588 24847362 41208376 47779248 50443940 42148724 27552896 249951792 23975352 31399300 6-Phosphogluconic acid 1323099 2439484 681286 153625 327969 663307 911147 75063 27277 38200 101589 540475 1234848 1053630 10063038 380069 1253981 Sedoheptulose 7-phosphate 16544717 33985264 15007709 5508506 6672661 17239472 19178208 5519113 4697487 5852050 9128590 13715090 32709426 23213958 139359808 12311577 20244984 Citrate 516644544 1057949952 363808512 441217824 385878880 421823552 590001600 285443936 297473248 266429872 373263232 299494848 535257536 586427072 1563259904 326879648 450276352 a-KG 38488920 77399888 30338852 46262640 22987268 37852520 45603312 19830480 19539756 15973742 28363184 19769698 32300342 40774748 128373552 24280408 16380314 Succinate 244890064 990918144 346444352 574207424 159856656 398876832 187628848 410825856 325499392 440717728 463554400 231071968 1767231232 1367043968 1872652672 408123392 164991280 Fumarate 168015968 415871328 209233616 294795744 154898480 169733456 277902528 102796176 146060384 233125264 278398688 163063824 232233552 434228992 1020489536 178681472 154631392 Malate 3604358144 8854284288 4832132096 5582373888 3704126976 4308620288 5194343936 2451381760 3721732352 5701744128 6773993472 4197372672 5252085760 9807814656 20193597440 4252018944 3903304704 2-hydroxyglutaric acid 240765040 825519168 260398896 278296992 249163776 373898112 177928304 305018816 243820944 144612320 139004864 226134400 350968224 804880512 804263296 285909984 240192032 Aspartate 1055731840 1476190336 781636352 1266182144 836472384 679047232 975629120 571958976 848326784 1230984832 1112811776 756839104 782186880 1143199232 2868512768 548496256 669335168 Glycine 68259800 106545992 57118048 31243582 36928052 60373144 73587328 32204002 45130420 40838872 105111984 41843624 63783196 54582116 157860416 41773148 37478568 Glycerol 73243960 99021328 89861336 43207972 34509376 84685680 81468648 61619548 53207004 52757584 33911688 59128916 85955800 66929504 316067648 43596280 52405924 Glycerol 3-phosphate 564143936 1060724544 480222656 250911472 270049920 565568128 600453312 272594016 238152832 349461344 236401712 419648256 828040000 715006528 2262191616 442825568 293300832 Glutarate 14431437 44466192 26085316 43003760 5991175 19447814 5313032 22010828 19069610 16114518 78457504 12001544 15402151 60868664 25226802 8397559 5896338 Uracil 43099168 61078876 61937444 121360000 39847292 27087522 34376548 40135768 54211464 103717120 86324616 49796976 65216880 60468896 98995096 15633902 23699420 Thymine 13530674 16347498 16985918 13188573 16005537 3712636 10228676 4218043 17774708 4800205 3542304 13351751 11655512 24446440 30974994 2396955 12398025 Uridine 193236864 262677264 317376032 188203488 106877824 230363440 191453088 224638512 183001296 238417520 190345920 280565952 355148000 146109584 720596224 134106504 139375904 Glutathione, reduced 1755520256 1721422592 1583189632 649499776 964865344 1734273280 1775248896 704842176 1184387328 997023744 1081554304 1270212608 2871291904 3527170048 8062845440 1974977024 1934427904 Glutathione, oxidized 31223032 102938944 26094398 17623056 23541216 26369286 29750114 26404576 19720642 22217976 19624424 24529796 55212576 42646844 258181520 23241062 31151010 CMP 26673190 24600076 19325264 10145936 18694758 15256873 14926639 9824421 17955596 17048254 24275226 19548478 14256870 22817016 57435980 9092801 13079652 NADH 4043572 13123770 3110380 4181453 4986809 11545408 4729106 10337629 10218804 12716837 9047429 5422194 8118237 14166142 12101767 7645597 4529963 NADP+ 3357187 6851003 3748773 1783630 1836469 3747216 2845240 3172994 3684371 2952860 3597391 3510369 1951523 2709891 10906322 1341561 2859912 NADPH 1324209 3103453 1263168 22942 553486 1867970 1249932 816162 1463816 1246077 723018 1176339 356000 1039178 6947540 366688 1817538 UDP-glucose / UDP-galatose 82560712 146521648 45100788 20150600 30173508 64986004 69313904 47957700 51073092 27047722 27206286 57198708 100358512 90304856 238876448 50079260 54746476 Uridine 5'-diphosphoglucuronic acid 43897508 40392416 26171820 12656164 24112846 26406326 16889358 13886182 27506414 7867397 22550402 27891988 12143989 54983488 169467968 31210834 36279032 UDP-N-acetylglucosamine 85376168 101022840 96928248 61732040 60039864 70777200 77434000 57915780 72271272 115419248 131307664 90567008 69535592 99039752 186122176 51145932 51451748 Glucosamine-6-phosphate 2812156 5421571 2037934 1848528 1340984 2581054 2619829 2013957 2158125 2018198 1500236 1680246 3337227 4228220 6432239 1677624 1585147 GlcNAc-Phosphate 0 0 0 0 0 5718 0 0 0 0 7659 0 0 0 0 0 0 UDP-N-acetyl muraminate 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Acetyl-CoA 1169654 890158 1674608 649039 586669 3117003 530068 731420 2134511 2106972 1299321 1516314 389411 537678 197791 485526 326333 Orotic acid 10477674 12608065 12531349 19543038 5879762 4950648 10127371 6640715 6565998 32464250 40434212 9302370 17159926 45768736 38087936 19607708 14476646 Palmitate 3229979648 2189756416 2274438656 1067930496 1837095936 3229257728 3272381440 1473772288 2061260800 1653724288 1898000896 2345625088 937142144 2530629888 5915772928 2055014528 2629861632 Oleate 769002560 590274880 741495040 371477120 544163072 713695936 573362560 351071616 587644800 267307392 255609056 752795968 224233040 231786528 2359258624 363161792 569086592 Stearate 876690176 573144256 745127936 529670592 571861120 1002561920 902027328 541457216 578117760 874611264 997961024 799515072 317304992 1077764352 1204598784 795790976 859489472 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name retention index quantified m/z PubChem ID KEGG ID Glucose 6.09 179.06 3565 C00267 Glucose-6-phosphate 8.1 259.02 3392 C00092 2-PG 8.33 184.99 3904 C00631 Phosphoenolpyruvate 7.58 166.98 3374 C00074 Pyruvate 1.29 87.01 3324 C00022 Lactate 2.06 89.02 3555 C00256 Ribose-5-phosphate 7.06 229.01 3417 C00117 6-Phosphogluconic acid 8.77 275.02 3638 C00345 Sedoheptulose 7-phosphate 7.76 289.03 7756 C05382 Citrate 8.77 191.02 3458 C00158 a-KG 7.11 145.01 3328 C00026 Succinate 6.87 117.02 3344 C00042 Fumarate 6.7 115.00 3422 C00122 Malate 7.48 133.01 3780 C00497 2-hydroxyglutaric acid 7.06 147.03 5610 C02630 Aspartate 6.69 132.03 3351 C00049 Glycine 6.71 74.02 3339 C00037 Glycerol 2.89 91.04 3416 C00116 Glycerol 3-phosphate 6.93 171.01 3393 C00093 Glutarate 6.76 131.03 3772 C00489 Uracil 2.25 111.02 3406 C00106 Thymine 1.97 125.04 3478 C00178 Uridine 3.14 243.06 3593 C00299 Glutathione, reduced 6.41 306.08 3353 C00051 Glutathione, oxidized 8.57 611.14 3427 C00127 CMP 7.51 322.04 3357 C00055 NADH 6.47 664.12 3306 C00004 NADP+ 8.36 742.07 3308 C00006 NADPH 8.79 744.08 4114 C00857 UDP-glucose / UDP-galatose 7.88 565.05 3331/3354 C00029/C00052 Uridine 5'-diphosphoglucuronic acid 9.42 579.03 3467 C00167 UDP-N-acetylglucosamine 7.28 606.07 3345 C00043 Glucosamine-6-phosphate 7.1 258.04 3645 C00352 GlcNAc-Phosphate 6.28 300.05 7115 C04501 UDP-N-acetyl muraminate 7.74 678.10 4292 C01050 Acetyl-CoA 6.41 808.12 3326 C00024 Orotic acid 2.52 155.01 3589 C00295 Palmitate 0.93 255.23 3548 C00249 Oleate 0.92 281.25 3978 C00712 Stearate 0.92 283.26 4692 C01530 METABOLITES_END #END