#METABOLOMICS WORKBENCH Jung_DNLI_20240919_115027 DATATRACK_ID:5207 STUDY_ID:ST003501 ANALYSIS_ID:AN005746 PROJECT_ID:PR002147
VERSION             	1
CREATED_ON             	September 26, 2024, 4:43 pm
#PROJECT
PR:PROJECT_TITLE                 	TREM2 expression level is critical for microglial state, metabolic capacity and
PR:PROJECT_TITLE                 	efficacy of TREM2 agonism
PR:PROJECT_TYPE                  	Preclinical Mouse study
PR:PROJECT_SUMMARY               	Triggering receptor expressed on myeloid cells 2 (TREM2) is a central regulator
PR:PROJECT_SUMMARY               	of microglial activity and sequence variants are major risk factors for late
PR:PROJECT_SUMMARY               	onset Alzheimer’s disease (LOAD). To better understand the molecular and
PR:PROJECT_SUMMARY               	functional changes associated with TREM2 signalling, we generated a TREM2
PR:PROJECT_SUMMARY               	reporter mouse model and observed a gradual upregulation of reporter expression
PR:PROJECT_SUMMARY               	with increasing plaque proximity. Isolated microglia were sorted based on
PR:PROJECT_SUMMARY               	reporter expression and their transcriptomic profiles acquired in both wildtype
PR:PROJECT_SUMMARY               	and APP transgenic animals, allowing us to disentangle TREM2 versus
PR:PROJECT_SUMMARY               	pathology-specific effects. Bulk RNA sequencing highlighted TREM2
PR:PROJECT_SUMMARY               	level-dependent changes in major immunometabolic pathways, with enrichment of
PR:PROJECT_SUMMARY               	genes in oxidative phosphorylation and cholesterol metabolism in microglia with
PR:PROJECT_SUMMARY               	increased TREM2 expression. To confirm these findings, we next analysed uptake
PR:PROJECT_SUMMARY               	of fluorodeoxyglucose (FDG) and examined metabolomic and lipidomic profiles.
PR:PROJECT_SUMMARY               	Again, independent of Aβ pathology, TREM2 expression correlated with uptake of
PR:PROJECT_SUMMARY               	FDG as well as increased cellular redox, energetics, and cholesterol
PR:PROJECT_SUMMARY               	homeostasis. Finally, we performed chronic treatment with a brain penetrant
PR:PROJECT_SUMMARY               	TREM2 agonist and identified a window of TREM2 expression where microglia are
PR:PROJECT_SUMMARY               	most responsive. Thus, our data provide novel insights into TREM2-mediated
PR:PROJECT_SUMMARY               	regulation of microglial metabolic function and informs current efforts to bring
PR:PROJECT_SUMMARY               	TREM2 agonists into clinical application.
PR:INSTITUTE                     	Denali Therapeutics
PR:DEPARTMENT                    	Development Sciences
PR:LAST_NAME                     	Suh
PR:FIRST_NAME                    	Jung
PR:ADDRESS                       	161 Oyster Point Blvd, South San Francisco, California, 94080, USA
PR:EMAIL                         	suh@dnli.com
PR:PHONE                         	+1 6507973837
PR:FUNDING_SOURCE                	Denali Therapeutics
PR:PUBLICATIONS                  	https://www.biorxiv.org/content/10.1101/2024.07.18.604115v1
#STUDY
ST:STUDY_TITLE                   	TREM2 expression level is critical for microglial state, metabolic capacity and
ST:STUDY_TITLE                   	efficacy of TREM2 agonism
ST:STUDY_SUMMARY                 	Triggering receptor expressed on myeloid cells 2 (TREM2) is a central regulator
ST:STUDY_SUMMARY                 	of microglial activity and sequence variants are major risk factors for late
ST:STUDY_SUMMARY                 	onset Alzheimer’s disease (LOAD). To better understand the molecular and
ST:STUDY_SUMMARY                 	functional changes associated with TREM2 signalling, we generated a TREM2
ST:STUDY_SUMMARY                 	reporter mouse model and observed a gradual upregulation of reporter expression
ST:STUDY_SUMMARY                 	with increasing plaque proximity. Isolated microglia were sorted based on
ST:STUDY_SUMMARY                 	reporter expression and their transcriptomic profiles acquired in both wildtype
ST:STUDY_SUMMARY                 	and APP transgenic animals, allowing us to disentangle TREM2 versus
ST:STUDY_SUMMARY                 	pathology-specific effects. Bulk RNA sequencing highlighted TREM2
ST:STUDY_SUMMARY                 	level-dependent changes in major immunometabolic pathways, with enrichment of
ST:STUDY_SUMMARY                 	genes in oxidative phosphorylation and cholesterol metabolism in microglia with
ST:STUDY_SUMMARY                 	increased TREM2 expression. To confirm these findings, we next analysed uptake
ST:STUDY_SUMMARY                 	of fluorodeoxyglucose (FDG) and examined metabolomic and lipidomic profiles.
ST:STUDY_SUMMARY                 	Again, independent of Aβ pathology, TREM2 expression correlated with uptake of
ST:STUDY_SUMMARY                 	FDG as well as increased cellular redox, energetics, and cholesterol
ST:STUDY_SUMMARY                 	homeostasis. Finally, we performed chronic treatment with a brain penetrant
ST:STUDY_SUMMARY                 	TREM2 agonist and identified a window of TREM2 expression where microglia are
ST:STUDY_SUMMARY                 	most responsive. Thus, our data provide novel insights into TREM2-mediated
ST:STUDY_SUMMARY                 	regulation of microglial metabolic function and informs current efforts to bring
ST:STUDY_SUMMARY                 	TREM2 agonists into clinical application.
ST:INSTITUTE                     	Denali Therapeutics
ST:LAST_NAME                     	Suh
ST:FIRST_NAME                    	Jung
ST:ADDRESS                       	161 Oyster Point Blvd, South San Francisco, California, 94080, USA
ST:EMAIL                         	suh@dnli.com
ST:PHONE                         	+1 6507973837
ST:NUM_GROUPS                    	5
ST:TOTAL_SUBJECTS                	26
ST:NUM_MALES                     	26
ST:STUDY_COMMENTS                	Release as soon as it is possible
ST:PUBLICATIONS                  	https://www.biorxiv.org/content/10.1101/2024.07.18.604115v1
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:AGE_OR_AGE_RANGE              	15 mo
SU:GENDER                        	Male
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	Pool_1	HSA-000019615	genotype:NA | condition:NA | group:NA | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_002; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_001; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_002; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_002
SUBJECT_SAMPLE_FACTORS           	2_59_mid_WT	HSA-000019571	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_003; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_009; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_003; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_003
SUBJECT_SAMPLE_FACTORS           	12_63_mid_WT	HSA-000019581	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_004; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_027; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_004; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_004
SUBJECT_SAMPLE_FACTORS           	24_78_low_APP	HSA-000019593	genotype:APP | condition:low | group:low_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_005; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_017; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_005; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_005
SUBJECT_SAMPLE_FACTORS           	10_62_mid_WT	HSA-000019579	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_006; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_016; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_006; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_006
SUBJECT_SAMPLE_FACTORS           	15_69_low_WT	HSA-000019584	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_007; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_022; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_007; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_007
SUBJECT_SAMPLE_FACTORS           	25_78_mid_APP	HSA-000019594	genotype:APP | condition:mid | group:mid_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_008; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_012; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_008; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_008
SUBJECT_SAMPLE_FACTORS           	18_74_mid_APP	HSA-000019587	genotype:APP | condition:mid | group:mid_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_009; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_003; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_009; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_009
SUBJECT_SAMPLE_FACTORS           	17_74_low_APP	HSA-000019586	genotype:APP | condition:low | group:low_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_010; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_004; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_010; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_010
SUBJECT_SAMPLE_FACTORS           	8_61_high_APP	HSA-000019577	genotype:APP | condition:high | group:high_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_011; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_011; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_011; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_011
SUBJECT_SAMPLE_FACTORS           	21_76_mid_WT	HSA-000019590	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_012; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_020; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_012; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_012
SUBJECT_SAMPLE_FACTORS           	14_65_mid_WT	HSA-000019583	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_013; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_028; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_013; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_013
SUBJECT_SAMPLE_FACTORS           	7_61_mid_APP	HSA-000019576	genotype:APP | condition:mid | group:mid_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_014; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_023; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_014; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_014
SUBJECT_SAMPLE_FACTORS           	1_59_low_WT	HSA-000019570	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_015; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_026; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_015; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_015
SUBJECT_SAMPLE_FACTORS           	5_60_high_APP	HSA-000019574	genotype:APP | condition:high | group:high_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_016; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_007; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_016; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_016
SUBJECT_SAMPLE_FACTORS           	22_77_low_WT	HSA-000019591	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_017; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_010; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_017; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_017
SUBJECT_SAMPLE_FACTORS           	Pool_2	HSA-000019616	genotype:NA | condition:NA | group:NA | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_018; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_014; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_018; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_018
SUBJECT_SAMPLE_FACTORS           	23_77_mid_WT	HSA-000019592	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_019; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_015; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_019; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_019
SUBJECT_SAMPLE_FACTORS           	11_63_low_WT	HSA-000019580	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_020; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_019; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_020; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_020
SUBJECT_SAMPLE_FACTORS           	3_60_low_APP	HSA-000019572	genotype:APP | condition:low | group:low_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_021; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_008; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_021; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_021
SUBJECT_SAMPLE_FACTORS           	26_78_high_APP	HSA-000019595	genotype:APP | condition:high | group:high_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_022; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_025; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_022; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_022
SUBJECT_SAMPLE_FACTORS           	19_74_high_APP	HSA-000019588	genotype:APP | condition:high | group:high_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_023; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_013; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_023; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_023
SUBJECT_SAMPLE_FACTORS           	9_62_low_WT	HSA-000019578	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_024; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_002; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_024; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_024
SUBJECT_SAMPLE_FACTORS           	6_61_low_APP	HSA-000019575	genotype:APP | condition:low | group:low_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_025; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_021; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_025; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_025
SUBJECT_SAMPLE_FACTORS           	20_76_low_WT	HSA-000019589	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_026; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_024; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_026; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_026
SUBJECT_SAMPLE_FACTORS           	4_60_mid_APP	HSA-000019573	genotype:APP | condition:mid | group:mid_disease | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_027; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_006; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_027; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_027
SUBJECT_SAMPLE_FACTORS           	13_65_low_WT	HSA-000019582	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_028; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_018; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_028; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_028
SUBJECT_SAMPLE_FACTORS           	16_69_mid_WT	HSA-000019585	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_029; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_005; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_029; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_029
SUBJECT_SAMPLE_FACTORS           	Pool_3	HSA-000019617	genotype:NA | condition:NA | group:NA | Sample source:microglia	RAW_FILE_NAME(Raw Source Name.LP)=20220209_BL_HEX-000330_Lipid_Pos_030; RAW_FILE_NAME(Raw Source Name.TG)=20220324_BL_HEX-000330_TGs_Pos_029; RAW_FILE_NAME(Raw Source Name.MP)=20220209_BL_HEX-000330_Met_Pos_030; RAW_FILE_NAME(Raw Source Name.MN)=20220211_BL_HEX-000330_Met_Neg_030
#COLLECTION
CO:COLLECTION_SUMMARY            	Microglia isolation was performed using the Magnetic-activated cell sorting
CO:COLLECTION_SUMMARY            	(MACS) system (Miltenyi Biotec). No CD11b enrichment step was performed as the
CO:COLLECTION_SUMMARY            	intrinsic mKate2 reporter is only expressed in TREM2 expressing (microglia)
CO:COLLECTION_SUMMARY            	cells. Hence, fluorescence-activated cell sorting was performed based on the
CO:COLLECTION_SUMMARY            	mKate2 fluorescence signal and sorted into low, mid and high expressing
CO:COLLECTION_SUMMARY            	subpopulations, as a proxy for the TREM2 expression of individual cells.
CO:SAMPLE_TYPE                   	microglia
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	No treatment
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Pellets from the sorted subpopulations were reconstituted on ice in 9:1
SP:SAMPLEPREP_SUMMARY            	MeOH:water including internal standards, vortexed for 1 minute, and spun down
SP:SAMPLEPREP_SUMMARY            	for 5 minutes at 10,000 g. Supernatant was transferred to glass vial for
SP:SAMPLEPREP_SUMMARY            	analysis by LC-MS.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	For each analysis, 5 µL of sample was injected on a BEH C18 1.7 µm, 2.1×100
CH:CHROMATOGRAPHY_SUMMARY        	mm column (Waters) using a flow rate of 0.25 mL/min at 55°C. For positive
CH:CHROMATOGRAPHY_SUMMARY        	ionization mode, mobile phase A consisted of 60:40 acetonitrile/water (v/v) with
CH:CHROMATOGRAPHY_SUMMARY        	10 mM ammonium formate + 0.1% formic acid; mobile phase B consisted of
CH:CHROMATOGRAPHY_SUMMARY        	90:10 isopropyl alcohol/acetonitrile (v/v) with 10 mM ammonium formate +
CH:CHROMATOGRAPHY_SUMMARY        	0.1% formic acid. The gradient was programmed as follows: 0.0-8.0 min from 45% B
CH:CHROMATOGRAPHY_SUMMARY        	to 99% B, 8.0-9.0 min at 99% B, 9.0-9.1 min to 45% B, and 9.1-10.0 min at 45% B.
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Agilent 1290 Infinity II
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
CH:SOLVENT_A                     	60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid
CH:SOLVENT_B                     	90% isopropyl alcohol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic
CH:SOLVENT_B                     	acid
CH:FLOW_GRADIENT                 	0.0-8.0 min from 45% B to 99% B, 8.0-9.0 min at 99% B, 9.0-9.1 min to 45% B, and
CH:FLOW_GRADIENT                 	9.1-10.0 min at 45% B.
CH:FLOW_RATE                     	0.25 mL/min
CH:COLUMN_TEMPERATURE            	55
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 6500+ Qtrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	Curtain gas at 40 psi (positive mode); collision gas was set at medium; ion
MS:MS_COMMENTS                   	spray voltage at 5500 V (positive mode); temperature at 250°C (positive mode);
MS:MS_COMMENTS                   	ion source Gas 1 at 55 psi; ion source Gas 2 at 60 psi; entrance potential at 10
MS:MS_COMMENTS                   	V (positive mode); and  collision cell exit potential at 12.5 V (positive
MS:MS_COMMENTS                   	mode). Quantification was performed using MultiQuant 3.02 (Sciex).
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	log2(area)
MS_METABOLITE_DATA_START
Samples	HSA-000019615	HSA-000019571	HSA-000019581	HSA-000019593	HSA-000019579	HSA-000019584	HSA-000019594	HSA-000019587	HSA-000019586	HSA-000019577	HSA-000019590	HSA-000019583	HSA-000019576	HSA-000019570	HSA-000019574	HSA-000019591	HSA-000019616	HSA-000019592	HSA-000019580	HSA-000019572	HSA-000019595	HSA-000019588	HSA-000019578	HSA-000019575	HSA-000019589	HSA-000019573	HSA-000019582	HSA-000019585	HSA-000019617
Factors	genotype:NA | condition:NA | group:NA | Sample source:microglia	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	genotype:APP | condition:low | group:low_disease | Sample source:microglia	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	genotype:APP | condition:mid | group:mid_disease | Sample source:microglia	genotype:APP | condition:mid | group:mid_disease | Sample source:microglia	genotype:APP | condition:low | group:low_disease | Sample source:microglia	genotype:APP | condition:high | group:high_disease | Sample source:microglia	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	genotype:APP | condition:mid | group:mid_disease | Sample source:microglia	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	genotype:APP | condition:high | group:high_disease | Sample source:microglia	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	genotype:NA | condition:NA | group:NA | Sample source:microglia	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	genotype:APP | condition:low | group:low_disease | Sample source:microglia	genotype:APP | condition:high | group:high_disease | Sample source:microglia	genotype:APP | condition:high | group:high_disease | Sample source:microglia	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	genotype:APP | condition:low | group:low_disease | Sample source:microglia	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	genotype:APP | condition:mid | group:mid_disease | Sample source:microglia	genotype:WT | condition:low | group:low_healthy | Sample source:microglia	genotype:WT | condition:mid | group:mid_healthy | Sample source:microglia	genotype:NA | condition:NA | group:NA | Sample source:microglia
1-O-Palmitoyl-Cer(d18:1/18:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
24-Hydroxycholesterol	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
24-Hydroxycholesterol(d7)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
3-O-SulfoLacCer(d18:1/18:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
4-beta-Hydroxycholesterol	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
7-keto-Cholesterol	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE HETE	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE HODE	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE HpODE	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE oxoHETE	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE oxoODE	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE(16:1)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE(18:1(d7))	16.025639642184274	15.780612504478182	16.974152803337212	15.90833256946513	16.67107581564915	17.30180952120171	16.183886882806064	17.65179739611172	17.031422939401367	16.16744975523072	18.033206396811174	17.13953696374618	15.847932331357129	15.916192974106309	14.851833448696757	16.974255429925208	16.12059625259742	16.534869998238765	16.190351936070737	16.69437366797257	16.38710925916133	16.698926143992512	16.51438993426447	17.59558742	15.955170941954957	15.962679840790646	17.112332621470223	17.456709910919827	16.029286364112096
CE(18:1)	13.50084187955693	13.14720196200707	13.94690627445331	13.436711530715709	13.49921609619402	14.70529111074708	13.659271023817666	14.84365218888076	14.403588860786689	13.370583396826973	15.173594181423129	13.622324964314934	13.299207988257622	13.250298402625315	11.387247488926105	13.58224078363397	12.476129205256177	13.092757140919849	13.767442864625169	13.931553796051308	12.677719641641005	13.117438521656169	12.376535198996633	14.582290196138386	12.479733967996566	12.250298417906329	14.311299872790965	14.358217102581772	13.007027266893965
CE(18:2)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE(20:4)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE(20:5)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
CE(22:6)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Cer(d18:0/16:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Cer(d18:0/18:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Cer(d18:0/24:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Cer(d18:0/24:1)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Cer(d18:1/16:0(d7))	20.728061493453584	20.677245433027245	20.776825587967966	20.694156497154353	20.726570117918794	20.755939279762487	20.63735121423753	20.89651914130549	20.774905749248944	20.73498965341819	20.912870730427912	20.89471010818446	20.77314262699108	20.672614916122516	20.55003282294628	20.83891892800169	20.75875927761707	20.72362551418738	20.656517006410734	20.79586818792265	20.56967752977644	20.83080471445805	20.73338408241197	20.851728204788014	20.801123964570138	20.79420201201501	20.840942931843358	20.87613679462071	20.81803595770001
Cer(d18:1/16:0)	16.730270101236453	15.436258346598214	15.490166234772568	15.511492006376319	15.167489772958666	15.823103520426761	17.058328334119686	16.796159995197332	15.547388624695646	16.955120512307776	17.333364599251514	16.37289974	16.437166490042742	15.064848841367976	16.703436799003477	16.314570339153892	16.638760049388114	15.977608772071733	14.87694944236414	15.67334330893598	17.158670177247817	17.24635767520336	14.931631354046162	15.364033391375576	16.831570796074505	15.687207538210323	15.418303233137499	16.34103163937282	16.92623112394134
Cer(d18:1/18:0)	16.778854750792764	15.320531054509232	15.772041536100405	17.69131422313507	14.876294386015017	15.352492024008516	17.512317603008764	17.43752532729983	17.63405864260923	16.549913651838814	16.439863593841174	15.954883985623578	16.196835565429055	14.723323906498257	16.35560368312891	16.036498182424943	16.77226609497808	15.907677274175429	14.262769418971986	16.916238470885645	17.02006572434589	17.06572889783861	14.75868334086133	17.53150205833799	15.85612785352066	15.601970120398141	15.21268788067562	16.219159199776808	16.572698895335844
Cer(d18:1/24:0)	16.703365837451855	16.103400075944975	15.71577970611953	14.425795471521104	15.665020156691982	14.76594130177019	17.11950812899011	16.66874463174075	14.93010162756669	16.064990264726756	17.502898782355444	17.069068216583346	15.927958643393492	14.601866976652666	15.72810133437656	15.329285576291019	16.656676767990824	16.352768314675494	14.572592455557242	15.780636135297856	16.960997258394183	16.752373210082137	14.801793214500826	15.050255151484093	15.173622267376146	15.18041620675442	14.810296482412594	17.134938248350544	16.337641851063395
Cer(d18:1/24:1)	17.78756030409215	17.147860840279545	17.034736682103798	16.30252024712857	16.59001364929946	16.445315765608918	18.317923766053283	18.083668272889827	16.293551768977522	17.776072642769428	18.325276923319795	17.872072401248374	17.371065357958948	15.93170709922752	17.76305126963471	17.281746036767796	18.077592389696225	17.48357085233816	15.658114260356072	16.810899685194293	18.390131736372062	18.36934663662224	15.780549151975894	16.177107192836832	16.90653911715737	16.77007021694213	15.876395118097017	18.082599742385838	17.947223400304715
Cholesterol	18.530313603558543	17.81879249803733	17.858791588420782	18.460213251245758	17.020974831281993	18.76704394533073	18.669587114409875	18.34762427	18.625098760140382	17.939807667530474	18.94454615795909	18.765035574851176	17.695802527554907	16.59036752508794	17.50493375357652	19.763505313367535	18.445741420327614	18.518414980281015	16.746270342636997	17.33161174536483	18.680879640552646	18.393944557575647	17.459694489169998	17.64542541424689	19.593189049422634	17.08478797275406	19.503533999734273	18.52662283348488	18.406321339505503
Cholesterol(d7)	22.960025278446885	23.03476804	22.98670772153683	22.791554687060888	22.97253082874393	23.031992797775768	22.918697028745985	23.142530869154015	23.001077097233022	22.820480690130644	22.980183454122876	22.869645756755457	22.940846093825936	22.88432432789717	22.83770564719861	23.021080641002932	22.896892848989243	23.067467523309478	22.966461611850153	23.086312673081704	22.96396960121157	22.994977310596727	22.90778257	23.086163173601484	23.129971676670767	22.97978725	23.095207528564035	23.06336152555753	23.009585131352033
Cholesteryl hexoside	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Coenzyme Q10	13.173675402043482	12.81858217748086	12.784634845557523	10.39231742277876	11.915736600622783	NA	13.659103920319536	13.542166136948524	11.09275714091986	12.250298417906338	14.827070734158257	14.358159413169494	10.851757718488935	11.29920801838728	12.092757140919852	10.976921775944582	13.414685235804388	13.436710263799338	9.714245518	11.392317422732635	12.562242513953503	13.521703485689335	NA	NA	NA	NA	NA	14.381058907450223	13.12023787734196
DG(15:0/18:1(d7))	21.39645154	21.158560623740467	21.27141690338802	21.332925268257448	21.19449106783424	21.77468724739177	20.93392784562641	21.666767427155385	21.430787942123157	21.081526046217437	21.521549098491047	21.37629959591289	21.26042313513538	21.053721378185028	20.687810821004614	21.260335506778645	21.23935796929841	21.480144162541762	21.036601295393186	21.31503016447805	21.03103777956222	21.481981169692354	21.10776189	21.263968782601527	21.384931709383196	20.95171969076069	21.408771512928915	21.63835856715785	21.39082421570775
DG(16:0_18:1)	16.072603108768195	15.01375300757473	14.41679629	14.976402552867425	14.655315857167713	14.963498822851115	16.358375643502765	16.21132401016114	15.027717923890892	15.919719232376798	19.359375013466913	15.760731327349792	15.687533014836193	13.580511596723383	15.472450911498877	16.56876276557978	15.812970872750515	16.17975757670866	14.053722942044969	14.476145236513029	16.453017929039426	16.60288623041243	14.18675994410651	13.978384229298888	18.191533002576072	14.374372980903587	15.654576735407275	15.741366450972336	15.831114337919667
DG(16:0_20:4)	16.384105076985747	15.511414173617057	16.00354514	14.908135509908476	14.860045229973046	15.428645312014853	16.87862956	16.640639341559456	15.154034847881672	17.134298362000376	16.514225990381547	16.646859521760625	16.506411105261385	14.369654594946008	16.70314546455588	15.687121942773224	16.09651504078459	15.797165899577076	14.109642452851762	14.05052890551542	17.304323511437055	17.40544793532049	14.732256478164317	15.256658354734395	16.059330550086425	15.485206672580247	16.029195761344997	16.26052034776253	16.28130723428962
DG(18:0_18:1)	14.510972480543105	13.199672344836365	12.30070848	13.621136113274641	12.669348947779682	13.851749035091297	13.811476920717212	14.21262164023923	13.861793165890411	13.90068335807622	17.897561574032917	13.555146606686922	12.946744059449097	11.71475424632033	12.692138698548405	15.384922508512513	13.93214747312344	13.985378868655413	9.737974585168299	11.71193565056499	14.18167010095707	14.250360601697247	11.977553143475928	11.97727992349992	16.57060705094822	13.031830143818715	14.210134683306531	14.062383540716501	14.26285129328639
DG(18:0_20:4)	16.389849770305126	16.594796365515613	17.125815820934555	15.463744734915485	15.39240195321486	16.127314875974918	17.27400488815351	16.661853236181987	16.317471264457666	16.69874372838163	16.367032910400273	17.47249762702573	16.58743121	14.659684379961787	15.231678701737813	17.13480946887063	16.20641917727695	16.57023140485932	15.007174473163255	14.732257669483548	16.30848053360637	16.488272948390264	15.682543671022632	16.251000368233054	16.736899992017097	15.396829263939406	17.092520372884454	16.50881249557292	16.466793421268196
DG(18:0_22:6)	13.385126829636274	10.422132517436456	12.208337513170777	11.784632848353404	11.948486574058983	14.199567671581729	14.423598175270445	15.16080400692572	13.72351142992135	14.618803127480907	14.829246653908324	12.328525154019479	14.961585195940836	11.718855719064004	14.28953942374484	12.874688537773688	13.788217986515512	11.97572481029981	12.718830040260364	12.744613163274007	11.926972090361442	14.73227966341165	14.986437665479777	11.573855363111688	13.819037733716664	13.666915889238268	14.67774095281924	14.581391454446466	14.173701979396233
DG(18:1/18:1)	16.25764017084321	14.847534705620404	15.077459871126013	15.257173506581504	14.806930130360799	15.820665983541714	15.841823038788608	15.826956362277459	15.562477628171315	16.12038067090873	19.99954102	15.951089058167812	15.685193059574528	14.509418248288242	15.609657331966025	17.093443455313594	15.916114464010468	16.309347460549603	13.869789366938601	14.883793512311977	16.060730086613212	16.119748455854825	14.679034725442854	14.747038783912187	18.461648981439208	14.832950856460892	16.08933539744598	15.780020213406841	15.673226610836888
DG(18:1_20:4)	15.305315824198935	14.621184202307852	15.029050785081507	14.133783961450275	13.900102159156798	14.57232600557266	16.215955341798455	15.640434752029588	14.696234696756582	16.284380535944123	15.895208656634104	15.73232908235858	15.526872090941668	13.036175499884132	15.876334679693832	14.962173031109703	15.092930531346225	14.723323906498257	12.714245517666123	13.878818362476757	16.120445299701746	16.47748255459754	13.60177078840771	13.900112062977456	14.962331534036558	14.51131039007398	15.02064805653002	15.668651236328053	15.186830765230447
GB3(d18:1/16:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
GB3(d18:1/18:0(d3))	20.808427739888113	20.745803595650397	20.952612112697604	20.96109741289933	21.05734831733392	21.03519760348772	20.725774458323258	21.06413736185275	20.925659734967724	20.738590186115022	20.869451530342147	20.886283154418088	20.924589133309116	20.624090933799714	20.532480974089232	20.913139095201846	20.947474088473083	20.84966637461395	20.809202697787157	20.95684297728639	20.942473198175897	20.968310452555524	20.856575026938255	20.995108924453266	20.864182072308825	20.958869286732074	20.883140846770814	21.008588235160328	20.951020325618998
GB3(d18:1/18:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
GB3(d18:1/24:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
GB3(d18:1/24:1)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
GlcCer(d18:1(d5)/18:0)	23.155203640342286	23.071089310870832	23.212382225950986	23.24690276287786	23.22043516826749	23.270492799697397	23.086633557902093	23.356084192860322	23.26920817054154	23.166033874934204	23.364960079937173	23.293950029637536	23.264745741695275	23.16194473376437	22.99693417094276	23.213345117044906	23.263390730463712	23.303547088364095	23.163459948816914	23.284481170483918	23.075475019561182	23.282723971002486	23.176048003322904	23.326624710199624	23.29400559431282	23.202626557016785	23.35613798391444	23.433806377982517	23.23588238005505
HexCer(d18:1/12:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
GlcCer(d18:1/16:0(d3))	20.153249909037225	20.072806983257504	20.243203436380806	20.157475028674437	20.109479654928332	20.07481637	20.044772309652384	20.235487854668637	19.86922860799404	20.080669495694874	20.168936805094706	20.158381776615656	20.117282804160634	20.06916814096854	19.865127670999797	20.13217602215247	20.050214668033835	20.177383762859314	20.109161655811505	20.155851798358622	20.08157569720008	20.16844004130485	20.083236414180387	20.194398132214815	20.184209922068813	20.03855681	20.15718254834791	20.258516379380165	20.21298228364031
Glucosylsphingosine(d5)	16.722319857250106	16.80864005420965	16.927298171372325	16.966622030147278	16.737071357610798	16.97318147154132	16.754952968581588	16.92009481517559	16.858182827165326	16.859248779212827	16.970288446965082	16.67767188	16.947393890875915	16.85020103778033	16.662460885695722	16.94279159164556	16.939710454570093	17.023466566006825	16.606988669957683	16.96079611902409	16.80358614637244	17.039664115894738	16.565149371215888	16.870599171844248	16.859652996767682	16.900075343328737	16.99109023225458	16.98866122782836	16.649890860530224
HexCer(d18:1/16:0)	14.732256478164317	14.436766188641682	14.021819762270734	14.015869319709958	13.14979947477312	15.154001614535476	15.532059130429426	14.999793265654578	14.480303241106714	15.180415620940733	14.876334679685572	14.741178040421179	14.640339140378998	13.915879378835774	14.977467762178719	15.250422778033752	14.784763651864546	14.381159604856858	13.801708358916462	14.521637827797719	15.452741824991058	15.299448018012194	14.346630074848767	14.225270711651534	15.388592668929363	14.458557364135748	14.947021371842927	14.749957393917304	14.414796208080718
HexCer(d18:1/18:0)	16.638099811699938	14.147204924942228	13.659197641928348	15.193411394179876	14.444830925029205	15.745706881579896	16.054398208419165	15.862951485434067	15.999853487507542	15.120904319059468	15.434438675582056	15.323290977205195	15.00717446714898	13.784720717706643	14.262222311629843	16.335242391342565	16.609772023254276	15.050764019840408	12.749869427396845	14.287195582321564	15.630984880655856	15.606952086259918	13.542064542283443	15.09296520693024	16.308585052380888	13.749869427396844	15.935180022851322	15.275204455455132	16.417913812303887
HexCer(d18:1/22:0)	17.620835467497795	16.127365664069618	15.880428080202554	15.39900483114619	15.386839328486355	16.645392014279324	17.34875166	16.902398758782482	16.093086564914664	16.106872056263033	17.356023678060048	17.084299672451206	15.908277032818292	14.741222583947167	15.58448914390909	17.264944978594002	17.521057091272212	16.588357569079577	14.650879039950311	15.41501533603459	16.928137109928898	16.522051211494585	14.572326005572664	15.16726916419518	16.748402376891203	15.133919234192003	16.570160487812753	16.886093055932655	17.46621604057641
HexCer(d18:1/24:0)	17.962321096533174	15.193379050745968	15.293143987970158	15.071973447575065	15.050671739552838	16.539990219801783	16.514483347163797	16.296590158900486	16.22269434418347	15.20516661561205	16.75254510112183	16.643067977384636	15.007211272383778	14.469710172686135	14.776194482756868	17.161286362875607	17.80702821564014	16.09278211293968	14.262520020030772	14.705336474949297	16.392634540872344	15.951099449014842	13.801708358916462	14.414796208080713	16.76347330250707	14.358156530304484	16.392669472764013	16.278425452030582	17.739543240387807
HexCer(d18:1/24:1)	19.66340946630991	15.587301865206502	15.23171019784725	17.380236866415377	15.44235650341413	18.964487601091054	17.46243324031106	17.43060143	18.693545626640596	16.401166186105783	17.79230760442563	17.639996300386784	16.029187577612976	14.659197641928348	16.014983543725194	20.00449299918137	19.516927205331758	17.060085390588313	15.0145157	15.640434672019584	17.32530685567004	16.858448995850598	14.999832685567453	15.939559013497053	19.920824373892703	15.557450537878504	19.622402874804084	17.365380194003713	19.652957872668647
Hexosylsphingosine	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LacCer(d18:1/16:0)	12.913937811710314	12.196212186225376	12.299283263963007	11.46269113350231	11.29920801838728	13.290406524271901	11.561975757994045	12.299203838490165	11.973031189594927	9.977138233	13.414782058190442	11.297110000846011	11.56223735376864	12.479778517021245	11.784634845557521	13.621135609005588	11.91587618	12.884172455932138	11.391882161486219	11.714245517666123	11.08222321485277	11.391616951717786	12.29886428162487	11.640244936179345	12.036173515556687	NA	13.346287439215384	12.092757137428125	12.03736410937679
LacCer(d18:1/17:0)	20.605245024802354	20.455091804989163	20.62676647531481	20.399984446770144	20.45224448564013	20.588513841172745	20.397677713484324	20.598512010183377	20.540822007455695	20.46723846400382	20.58487514554186	20.526557195502697	20.556987362184238	20.47124743844775	20.33103608721374	20.609264657387065	20.497315786182444	20.448089665089583	20.37464379	20.61953719097484	20.357971860081264	20.68836467106099	20.57337580645897	20.66943471	20.657085847518672	20.558806574272026	20.53418252446806	20.690134924410174	20.56431133598388
LacCer(d18:1/18:0)	13.71172167226024	11.266015247349667	11.807958094598462	13.134372663934261	NA	11.966988022856562	14.446649200664524	14.104299072007692	13.440240810510698	13.86960458123394	NA	12.347994436614163	13.331057336283115	12.34673200559143	12.478440202997865	13.082908055610568	13.225436088652378	11.75787036596997	11.327383194523847	13.17227178373826	13.925991354413727	13.809969832274737	11.082187849434083	13.744043432908486	10.56258356763683	12.344853975759992	12.75097937331532	12.768028088788117	12.654901140883817
LacCer(d18:1/24:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LacCer(d18:1/24:1)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Lactosylsphingosine	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LPC(16:0)	21.260050593241715	21.110020398302442	20.69950010154093	21.247652276767262	20.26493504880888	20.39232457993404	21.028329125088995	21.690217298000423	20.841635924491587	20.836840187918146	22.381082884851683	21.647122238217076	20.781215415073312	20.735456548961047	20.87523985618112	21.640739989171678	21.191453425551444	21.10233920412719	20.378210920654105	20.73057339006975	21.758628485059873	21.117567777649562	20.694748106469504	20.776035112783504	21.75471358982174	20.661216998036807	21.663366994715286	21.123601426155183	21.197002447299674
LPC(16:1)	16.820777307964867	16.822503336720985	16.31888242235939	16.48348173985534	16.196801233881462	16.117126502886656	17.008818079474267	17.174832587543694	16.48654842816498	16.820891843878865	17.430901643777283	17.23611996674569	16.82757134708313	16.558658925619497	17.150159759164865	17.083763245658492	16.826223255619205	16.865108933968948	16.10659401716702	16.439195081475262	17.45369556921899	17.217589123554593	16.709928553217733	16.46042261795594	16.894907285791252	16.481311289592227	17.014375510520644	16.857298295918525	16.96950781237456
LPC(18:0)	21.195372390870546	21.207944225681402	20.524368787817206	21.475456007657584	20.55680237246164	20.344565185667122	21.11546067290526	21.396915277699414	21.12629287018066	21.101701786109224	22.08979291966988	21.652353405405382	21.016649923146296	21.09464709450791	21.04614444830861	21.739222441379745	21.341959101426625	21.403848778219018	20.41297749321013	21.162646253585557	21.331426243619276	21.30359727	20.950592861721365	21.199254795963572	21.77386528873381	20.55073880628955	21.750416684706483	21.04838428	21.37453752532009
LPC(18:1(d7))	25.871641032563147	25.76877583022179	25.883695792444186	25.98391464186726	25.85642908	26.008521038931754	25.92439613	26.086387348935713	26.10486507865767	25.976820181162047	26.140346873254487	26.10256188215737	26.018536963014906	25.99308974064059	25.63804794784649	26.100294440863163	26.00474590449955	25.97094928152076	26.058724493591736	26.032853875482765	26.05995104115394	26.06013669524172	26.024216700439485	26.135916322966928	26.005219006629723	26.072944854388417	26.084604012621572	26.176913239275475	26.04747388835988
LPC(18:1)	20.964982217788506	20.916224184641223	20.655032583761212	21.049557232072292	20.47197311927184	20.525957202357976	20.77121310632336	21.14081938519009	20.886844630277828	20.91875645976041	21.469267828122515	21.22167549904983	20.871097405819686	20.798502646800117	20.876646967472592	21.284327472190842	20.996305912520352	20.99320797889735	20.496877360129098	20.768763674703003	21.060427546040188	20.996090075908235	20.83083311342775	20.906465457591576	20.98479319518287	20.55948982938632	21.138648207282984	20.79248266424291	20.901482534082646
LPC(20:4)	18.389539713132553	18.80681969	18.248934830552837	18.414279779405508	18.239163147966238	17.300164875301586	18.73696126692391	18.758803735082513	18.130505994220513	18.815708830986317	18.56406068998093	18.987447768936576	18.628847233070665	18.191869891543714	18.606352776666938	18.16377023430511	18.568849840616707	18.75271673931059	17.701340676551478	18.496767646494803	19.08716340987996	18.893792128800932	18.249372232948723	18.244757525194387	18.118609977320634	18.105609485471216	18.322414310203207	18.298256965219288	18.590464415263643
LPC(22:6)	16.09872459805504	16.068496566836174	15.77055540212309	15.726872716678933	15.647879125615395	14.791069580615838	16.213586291647754	16.200070435430543	15.718189853351602	16.54265698	15.434899838168878	16.284321092773368	16.18758571	15.552317576997924	16.163841960285055	15.953871309851541	15.83049638921342	16.363970216862903	14.742121799716545	16.01794552425151	16.73005487411937	16.393785112112614	15.752933060684072	15.93870668768138	14.962213621178147	15.492642454378032	15.467368658377946	15.529395112616434	15.886852704339832
LPC(24:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LPC(24:1)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LPC(26:0)	12.915879378835776	12.147204924942232	13.250289195091822	12.43671154213721	12.946906274451772	13.198734101558891	13.521600439723727	12.60177078840771	13.458406613236532	13.064742764750259	12.780785036701216	13.696226939259518	12.520490020362669	13.092615214743931	12.521600439723732	12.43670559330343	12.677719641641014	12.94690627	13.323054760341646	12.343898621374585	12.15828727971568	13.323054760341646	12.97728395	12.47976051161716	13.199104884127376	13.369597346278677	12.299204656283523	12.251984621737623	12.240100656804579
LPC(26:1)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
lyso-GB3	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
lyso-GB3-d7	18.37039179708558	18.2454251	18.402679713671922	18.365453749663036	18.408096112918592	18.46079591824145	18.362354926706182	18.635629510234157	18.385704973240742	18.253052331386513	18.46182140262199	18.464250950901356	18.325163733817668	18.21980511053513	18.067847625604877	18.190308497594174	18.327453087329406	18.363874533247337	18.329268050800454	18.235391669989074	18.23602565361034	18.36472238	18.352444068306852	18.430524687764827	18.35969536173087	18.296367506055823	18.37741043046286	18.510911452561768	18.302136232430428
lyso-GB4	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
MG(16:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
MG(16:1)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
MG(18:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
MG(18:1(d7))	15.691875228059951	15.317306081839535	15.38064758588402	15.647624050290702	15.381162188560273	15.767595835015612	15.42041687123025	15.753033708346122	15.333986174836866	15.636160552823453	15.485312109645191	15.363177869111958	15.029050734571516	15.323296883457783	15.55241573917117	14.922232316399123	15.743080265431454	15.35283027364992	15.113927517455279	15.44877902620951	15.269018531423235	15.425988194166422	15.320656571780834	15.42576628392188	15.602014428963905	15.625547794232341	15.542293083265715	15.16076125958009	15.452860019093245
MG(18:1)	11.51935225430429	11.980654607354557	NA	11.088804524642152	11.179772524566403	10.977279382523903	12.436711542137209	NA	11.294637456755757	12.67454204931386	13.498181834694615	11.707048618600062	11.476216898275192	11.19894778506579	13.120237877341957	11.709669936982147	12.147606968948407	10.970651820434274	11.104570881386898	12.521519359849929	13.03850216996364	12.587978837370702	11.71406526673653	NA	11.909501050464247	11.19967226477987	12.09209027870629	10.848168818146684	NA
MG(20:4)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
N-Oleoylethanolamine	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
N-Palmitoyl-O-phosphocholineserine	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Palmitoylethanolamine	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
PC(15:0/18:1(d7))	27.364737435653137	27.33410498394641	27.398731457678718	27.35236836	27.356177585171555	27.42501788699784	27.32397657474317	27.440595696199157	27.43963571358017	27.34234766	27.414672952430593	27.413088813914726	27.36994020220499	27.34115829732856	27.254332807650137	27.403017598446684	27.378661280375454	27.348344335128182	27.406507127721394	27.405131406373982	27.390296560570512	27.39540570566883	27.344778395448824	27.42826177026793	27.40793893353761	27.400720001739383	27.44228579965408	27.459130660462286	27.38820391847186
PC(16:0/5:0(CHO))	13.838406682700182	14.432795744918964	14.036967332011878	13.814878955384314	14.261971971975456	14.724021294855564	14.016617456517258	15.202553974914188	13.026865086584753	12.736342510833525	16.13023733146429	14.941759879715773	12.470185892643451	10.06776869271491	13.472359656758805	12.596174843029814	13.360361105572812	14.188700589928922	12.23111368709168	12.564808274453899	13.283934590448377	14.093868344138967	11.36921230555491	11.500284444074813	14.175917991328731	12.347196403299147	12.888310510566331	15.645834298400766	13.54801583935905
PC(16:0/9:0(CHO))	15.282008112459604	15.212273806405694	15.29106157289466	15.266730962419595	15.176435917184627	15.550194995766963	15.113964402471959	16.09627703125485	14.581685597324094	14.018712332950638	15.806617665677953	14.915047729518601	14.471701185120638	13.860258465680813	13.726780151278573	14.558217911806258	14.14474316672713	14.856758788153146	13.746343190471828	14.382018321456433	13.941293216664283	13.626448193356701	12.657888203662878	13.677767418777025	14.653242337837158	12.533865864918488	12.363616962662901	15.654405965628827	14.53218582528644
PC(16:0/9:0(COOH))	15.351540524830426	15.128701635939757	15.423256487266098	15.43299664539806	15.49245143992439	15.50741948163808	14.816021656963468	18.151991095826713	15.703004701703717	14.95237131636706	15.190457358816726	14.757047921370052	14.014481139048463	14.266914750253967	14.309433782769357	15.465277024251561	15.104236783326805	15.296815792754742	14.219991769670331	14.416434617409896	14.797394452313828	14.973138053445153	14.075194330374286	13.83381916255422	14.54913352891236	14.488576559145404	15.220418406358828	14.353855952156652	15.020288100441316
PC(18:0/20:4(OH[S]))	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
PC(18:0/20:4(OOH[S]))	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
PC(34:1)	24.638986897263766	24.138394933652773	24.04826437112843	24.130262207653065	23.632154291980786	23.990077065314296	25.466056235870322	25.09491054135905	24.04042243063866	24.98941869164026	25.12904305428845	25.162592355236153	24.525761936156446	22.46083581267111	24.700820194816224	24.712275482680933	24.689004568797078	24.65622018351157	22.60706927895486	23.599913908338745	25.409363879440264	25.19007245258355	22.872791667400755	23.629652266675635	24.57755157624092	23.99866499	24.71464093182818	25.091836143512662	24.748419554568365
PC(36:1)	21.934060400032596	21.084847502152098	20.961732497367528	21.62492241210496	20.627776176987265	21.923625296885717	21.984288449872874	21.826901424340402	21.855325674511562	21.09396670752943	22.168282678959336	22.146131051797983	20.894327551041325	19.62203529971892	20.65349460294649	22.77043200766752	21.998281265049016	21.74191897901122	19.719090591648705	20.680046998045572	21.33806757693076	21.438639524072258	19.88057190423652	20.79074959197916	22.57751573823138	20.382953358202954	22.758027988933787	22.126029049773422	22.03957069087081
PC(36:2)	22.059731327490777	21.262056215300962	21.217800890610626	21.40123909412179	20.76401311829908	21.353083591808037	22.590306571529695	22.296490089026467	21.179303850916178	22.512765115078853	22.295437793510985	22.18286477625634	21.74943153383085	19.793973766718025	22.23563182717118	22.112988990535534	21.976045104263264	21.661820213912208	20.15594704098647	20.880064968359605	22.816038511084383	22.780802128472413	20.28987662046071	20.61739646390535	22.05156594063359	21.33316156327461	22.036956945445283	22.311985688358064	22.004977871353518
PC(36:4)	25.96095415783786	25.837906978116486	25.845237647070224	25.071984052645274	25.447586077034774	24.634767990972097	26.741312625399686	26.596633593337497	24.711703272559134	26.439147545414514	26.602640167704813	26.605356785631507	26.040027135200706	23.979441935928186	26.14216957290596	25.00557924929897	26.01166747692227	26.20383565535117	24.25256823219589	25.055881072101197	26.865711577738576	26.612314641326364	24.35769090479772	24.939752651223326	25.11050208583332	25.69410110439965	25.27815720879245	26.67348493	25.988187698726737
PC(38:4)	22.69782889867154	22.920605404142247	22.85592880386083	21.88590329879486	22.316055954741206	21.622760899113842	23.763853913595923	23.644684880770864	21.74225586280571	22.848866160389203	23.791302723172024	23.795217405972416	22.759576754508675	20.896379035391554	22.385130522245657	22.28052974384205	22.718629725370256	23.314477981839687	21.055486073688666	21.93669746149388	23.194683957609683	23.098015237320187	21.24347987529381	21.699440286423417	22.059940782682077	22.21967143514112	22.168291373269845	23.877723051380983	22.731640020067644
PC(38:6)	21.205621673989782	20.807449394808586	20.76462209225997	20.876256800025075	20.356683688855057	20.385375269384873	21.97220655107173	21.741142153132753	20.58546435496652	21.49378975	21.657485379580002	21.69332648	21.140432681272525	19.133872242489613	21.317654456796458	20.826276676192258	21.119935454910777	21.345945505656093	19.175945619224432	20.37515740736992	21.923147214934023	21.856145428915134	19.623138020719175	20.478909016476997	20.52780649870201	20.69437770068946	20.797003561265452	21.80481798575207	21.289384680092184
PC(40:5)	17.499077101563486	17.488416414322845	17.27487187830823	16.87538392514946	16.879587287791853	16.93110841675023	18.318363817093804	18.14047685536372	16.96829728424668	17.48411413463175	18.23068677604326	18.420640187816655	17.46366368580381	15.938447709513305	17.1451418	17.573505308316857	17.545976471412544	17.84051529158944	15.885437701797402	16.765358043670002	17.752954950852434	17.853650417585794	16.075462223603477	16.61585094	17.302328708271126	16.875356105326908	17.424259705657782	18.178417641227497	17.47308267
PC(40:6)	19.86689987	19.96533934877787	19.769108105115915	19.830749264831628	19.298504149327695	19.24137229353707	20.653332603800937	20.527084094902925	19.728101243798115	19.711482946554035	20.610638387851683	20.70238634296885	19.729202599974577	18.096598394271634	19.40312118793158	19.883355451994575	19.93049860658937	20.385388777987966	18.116267680681535	19.48108728990532	20.03991534	20.057104605377905	18.363460048631165	19.422284458888015	19.578164203495067	19.442067481628452	19.81434772947564	20.758683757504695	19.83082654
PC(O-16:0/0:0)	17.093414133812445	17.21671698865806	16.97648544656656	17.05174478571949	17.269386396048667	16.404423394870143	17.27142175875005	16.741228187310607	16.967843194929294	17.173982116988476	17.273635041470286	17.140524655866912	16.897269182623713	17.34846913235368	17.441258256703485	17.311261899198247	17.25253918026705	16.862363583933888	17.32367156098265	17.25227709631085	17.33386611847908	16.884096551081445	17.05280170544173	17.27416649218448	16.953147071241766	17.587751916455844	16.894886037012228	16.56738642227402	17.201121154795594
PC(O-16:0/2:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
PC(O-18:0/2:0)	14.605301077204587	14.111816892962354	13.519618809156402	14.491178258811399	14.429249764112228	14.050501839712611	14.189187601964447	14.559000190667208	14.668267757700296	14.478833636318223	15.319156839563826	14.859331795595438	14.05283552	14.15483371639869	14.57171518205482	14.890464637098598	14.911943718393584	15.083557156475116	14.144849356214381	14.120027052445785	14.733567173304413	14.348500500206965	13.959241231583784	14.10990644937987	14.85216046	13.63216743621424	15.11559678	13.900753994254718	14.905190106635581
PE(15:0/18:1(d7))	22.583039700306497	22.53582049455672	22.575975287991476	22.589605039906186	22.583703625428466	22.69198214493459	22.501970896575113	22.653469759784265	22.598129901599535	22.517242772642344	22.60859238	22.61456589406894	22.546789173168946	22.53014859270271	22.303501915433102	22.51586787759604	22.529388059441324	22.57609280474523	22.53295473710574	22.632348392184777	22.538926028109447	22.549137159710078	22.61679161679867	22.669103869368907	22.51816277392281	22.628113990131396	22.679329451985378	22.605637437397682	22.605063138723423
PE(18:0/20:4(OH[S]))	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
PE(18:0/20:4(OOH[S]))	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
PE(34:1)	19.134563331468602	17.31638293740803	17.02408377730717	18.239781986866603	17.120942561917428	18.392369117624124	19.481678093359868	19.046731096607378	18.402629321544907	19.357280504197313	18.411540339241903	18.642229307904213	18.674143874298956	16.794064490345416	19.17303407377819	19.24363744	19.089277655220304	17.913926496774987	16.369994620950997	17.54062410688666	19.83516564	19.59973739411384	16.305949663520238	17.71518014464431	19.10834099961335	18.133319589468993	19.063339420261272	18.43626669784594	19.007562749467795
PE(36:1)	19.33270001440739	17.730991796688585	17.605188926288147	18.34111935826077	17.409692306994256	19.15848396232843	18.9273967	18.790061575365275	18.808624788179298	18.053569792520104	19.009130588247867	18.961296432159585	17.786711500846472	17.04576880619123	17.55156743	20.031827073915963	19.422425757972253	18.316284055954863	16.962732540955024	17.25904067454152	18.483120699703623	18.393599330933228	16.825345124913326	17.48411389710409	19.916863571826973	17.18717130628011	19.480793919282387	18.90040754186003	19.345522555917952
PE(36:2)	19.190994976134952	16.666229763460407	16.916289029834306	18.515914630632256	16.423219012399464	19.24355493441216	18.862008670032058	18.48884365758811	18.944627027774384	18.781983587728035	17.95909393927531	18.24545209296834	18.03881763194831	16.564957444500706	18.851961649303846	20.299044642621347	19.10520065120853	17.715440950905716	16.99815803013242	17.395540094162946	19.24936050858989	19.143502768690098	16.73684801432494	17.267996136704785	20.143871659768482	17.54654184076137	20.030385578179096	18.127816454003586	19.066440349496236
PE(36:4)	19.444896412013577	18.489172523758732	18.613572566231635	18.20650243074182	18.279492990773107	18.12449415680241	20.28885399085096	19.864879746888548	18.22286635841719	20.262649273586156	19.418186407026873	19.622076520728744	19.61944490338731	17.24131857159763	20.009497707011093	18.537048597695613	19.452360323792856	19.091343037426395	17.082954811378368	18.184935603859916	20.618387589836566	20.530414944095018	17.252823287739332	18.11731954317953	18.430133362809674	19.158905000852965	18.72613313816038	19.59071277202971	19.364968206306585
PE(38:4)	20.891598629486598	20.30140280834452	20.46466876027748	20.13048173	19.982990652937612	20.111851531501454	21.633000883241657	21.345802697309324	20.29692409825156	20.83971332	21.349994467910314	21.406883414844057	20.735721939910547	19.13870210668085	20.360106492560732	20.789734673963466	20.68707283968005	21.00638354223208	19.345871252262214	19.873352906846293	21.164001644231384	21.149330683781464	19.22433222	19.84667311148177	20.55369083275311	20.110758705149816	20.619236279177038	21.418134111078743	20.79537703436094
PE(38:5)	18.83213907	18.167996903589703	18.045852514395374	17.968067424562026	17.775072313937375	18.191325905067238	19.353296801462893	19.15529502228643	17.92716118472016	19.062257502349986	18.901413532998625	19.330157009007557	18.73813937033039	16.592318375288052	19.133597666559062	19.117223617054368	18.81276518349235	18.690836103734817	16.790852321952862	17.47932001455893	19.63506233216533	19.548876292062438	16.920683534395632	17.31933043610983	18.790174133569522	18.107969424923294	19.028866459844508	19.042445123999727	18.866866720988547
PE(38:6)	20.670912403677132	19.529584742888936	19.697874338837533	20.110092790580623	19.300299277153098	19.846211691465083	21.288882563663503	20.950500779225578	20.115605016271548	21.216346723784863	20.33361714122786	20.607150611732028	20.585532024779162	18.500652254578767	21.101699846703635	20.760879926686222	20.699084670624416	20.29483524	18.35523952872024	19.719867007938973	21.529331494659775	21.478826367305434	18.707475062814467	19.76626237	20.039947169090397	20.218113460116683	20.41482678641506	20.496960048899645	20.714040994817832
PE(40:4)	18.287314477032027	17.131049458178897	17.006988197780622	17.746428668020535	16.37866670978848	18.157277349228025	18.77596454408168	18.37990380429303	17.987596912285117	17.779262904256505	18.355558068840516	18.279568517729416	17.569139626123132	16.67204608945862	17.27629481	18.785490359928712	18.15330819114925	17.893610220446025	16.732207623306554	17.401646751550786	18.13473548680859	18.080404052339677	16.902136462614894	17.389784110374386	18.53880285925642	16.828721024448065	18.358086132379757	18.17149194746428	18.07613563498078
PE(40:5)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
PE(40:6)	20.397243602955413	20.132992821972305	20.299031968658714	20.20864054248563	19.858233600795298	19.874038757707442	21.21995347114404	21.145637888134353	20.282989217389115	20.095720592868144	21.094502895318257	21.239983350920692	20.283169645919763	18.736913343118996	19.817662593523764	20.55483892251538	20.468939516727048	20.871709429982676	18.819960559118755	19.76216651249143	20.558504006435808	20.647400173705073	18.86481678331251	19.65263669938397	20.138503419927076	19.62386691547061	20.305892477646715	21.111157279483706	20.472884632982247
PE(40:7)	19.506416789827604	18.55653698267268	18.48272693711619	18.747315230682403	18.307659249603923	18.58649887148294	19.993652291440455	19.795436815126834	18.732265697317775	19.92275117139718	19.359155539266247	19.44475571073752	19.442261917493667	17.244924690477397	19.73165186716372	19.233858454599712	19.576732375292952	18.974413143286945	17.277945271820933	18.172564866663574	20.27357769203458	20.323888797533325	17.642080504245335	18.402383720396934	19.02247038725058	19.100511540589505	19.215901966462717	19.41080687873193	19.37224870228126
Sitosteryl hexoside	14.366190717865821	13.977277324992993	12.147204919084794	12.147204924942232	11.092757135170224	11.784634845517045	13.14720492494162	12.85174904141606	13.250298417906336	13.458406613236532	13.199672344836364	10.983385243586003	11.479778176710434	13.225326454079864	12.88410171609411	13.346513733165635	13.962173031109709	11.915731886883597	12.784634845557523	12.884170519108434	11.09298568590262	NA	10.562242424221083	12.784634794277984	12.39220795326688	13.915879378816218	13.640339847247734	11.562242424188494	13.250296547028363
SM(d18:1(d9)/18:1)	26.11855942215299	26.114568375584682	26.174139241557725	26.150465518861978	26.181936656019804	26.179970067829455	26.10874159321157	26.26186377916953	26.17461424259536	26.088740376906504	26.230673216405602	26.262255173469907	26.17492768	26.008942902212198	26.01035133020401	26.234919697482017	26.165459562502587	26.127818544977124	26.162934937622257	26.17088321421515	26.156313253985953	26.176038873968096	26.12198423840845	26.175142818583755	26.21290034976956	26.161164625495655	26.26179848612328	26.23200041	26.11609293435646
SM(d18:1/16:0)	22.07899524947019	21.556001138426403	21.534684098869242	22.245979865748986	21.289073794108795	22.157407786436814	22.145801565682895	21.906638854886744	21.949397796192528	21.9625682	21.903974557090642	22.036715073453422	21.605551973632302	21.529927085925987	21.662829488034244	22.894206030601115	22.18034013708325	21.728673604427303	21.606918126763013	21.815142997092657	22.264796956531068	22.057477654626144	21.837229232964727	21.633653098025636	22.686243578148506	21.33832121663612	22.161249940256177	21.808182417858177	22.04686003884368
SM(d18:1/18:0)	20.009199133306517	19.37550228086533	19.333894384726722	19.501242505654705	18.808832994981913	19.467252540132343	20.318214068454225	20.13567573737359	19.382744014129027	19.410866236803717	19.985977077415537	20.308049749471838	19.204087738415414	18.186540471419992	18.889262416934685	19.973216908243085	20.034978884440054	19.952693542116858	18.155273578425124	18.840380492834438	19.706576377345616	19.54074926895371	18.545219822125897	19.045639789992524	19.774506660237016	18.570019518871383	19.75164275005162	20.13675237503586	19.948501745067677
SM(d18:1/24:0)	18.655103821605913	18.43298500603938	18.157303866375894	17.256655720423172	17.845638520975825	17.66138906376687	18.79296224369694	18.63190787881432	17.62337042666809	17.652457992246518	18.899602000327963	19.104070768613536	17.78999466474882	17.117885358127374	17.500985396784333	18.264740118784072	18.419186366219908	18.669189117641142	17.077010117908834	17.49967857274827	18.35517761240918	18.18552006470251	16.80577147432702	17.29913949686504	17.80748600052947	16.943775454325166	17.72166347086923	18.984490281904094	18.434579149410823
SM(d18:1/24:1)	20.73911901496357	20.40769239407014	20.334201922161792	20.146973766955757	19.865560111488158	20.256927842504222	21.08578653465816	20.781455037000327	19.981950603106803	20.378309128203288	20.821353429842585	21.158449631987924	20.24705577902872	19.484973910301214	19.86296341321547	20.98782121500304	20.732614035095718	20.82298643584625	19.46583328779904	19.732600980571807	20.67145542829052	20.53327114408522	19.557595833004633	19.484186215301644	20.810978150770296	19.46980900441879	20.59434978406204	20.893410157278463	20.697284
Sphinganine	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Sphinganine 1-phosphate	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Sphingosine	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Sphingosine 1-phosphate	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Sphingosine 1-phosphate-d7	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Sphingosine 1-phosphocholine	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
Sphingosine(d17:1)	18.109936677486388	18.138419463690305	18.06704326050909	18.027674066055162	17.866553075958723	17.858346589881943	18.07614863726003	18.089143441696745	17.926518827720823	17.992295517664406	18.207399798928066	18.01364090121132	17.851389848023803	17.888192640354422	17.974587470433786	17.78582936	17.892335948125528	17.81896450170762	17.769517793431966	18.035187517503864	17.86994226417355	17.85503674220003	17.945751710945505	17.85281749113984	17.85843863170556	17.931732492686155	17.90313801024336	17.933690575145693	17.92884289183648
TG(15:0/18:1(d7)/15:0)	20.834464985052335	20.44321277520579	21.596204056810837	21.152827115570332	21.465672272700022	22.066622952313246	20.332746111187248	22.502752539299014	21.826659267740837	20.588122802332045	23.427029979506827	22.250027452627066	20.653198345733102	20.40262176	19.17732201	21.64974618167914	20.75490891685795	21.02074902334858	21.047935643953192	21.30136367266243	20.693959372909866	21.473287030122087	21.115034931496048	22.36627750584206	20.789998577363754	20.73094012586498	21.93411689862742	22.042507134643582	20.749594792278263
TG(18:0_36:2)	12.59692024	12.996060675598697	13.931102035486555	12.921938490590852	14.151404679925434	13.202655350912451	13.832276467386443	13.842980733646623	13.57250922	14.05582268969068	13.919693575293419	13.88301481624746	13.763932841713398	13.31910472031938	13.454406329055784	12.764414510261007	13.921966327591655	13.47436751182095	13.369776191011615	13.960124412831888	13.288820613207651	13.856963398417882	13.435081816851017	13.293762683424633	14.163789136437106	14.637440764219724	14.415584653442384	13.807939512330952	14.388041814970954
TG(18:1_34:2)	14.48060084167219	14.356956562263024	14.615152590126268	14.281463322338006	14.772457291839736	14.254024879821547	14.78831622643687	14.416152813305038	14.78518544981738	14.933350209224045	16.16095599508391	14.537173244242547	14.458949834097323	14.069815451688882	14.186181045111667	14.935097008943636	14.682755496610278	14.50492962647119	14.447367059842763	13.95425290313582	14.178446563740465	14.33313282036155	14.693990653180977	14.732189557620378	14.725318225068355	13.908043219257303	14.415944395863226	14.26243866427215	14.681059858011656
TG(18:1_34:3)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
TG(20:4_32:1)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
TG(20:4_34:2)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
TG(20:4_34:3)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
TG(20:4_36:0)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
TG(20:4_36:2)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
TG(20:4_36:3)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
TG(22:6_36:2)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
TG(22:6_38:1)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
TG(22:6_38:2)	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	internal standard	Expected RT	precursor (m/z)	product (m/z)
1-O-Palmitoyl-Cer(d18:1/18:0)	Cer(d18:1/16:0(d7))	8.1	786.8	502.5
24-Hydroxycholesterol	24-Hydroxycholesterol(d7)	2	385.3	367.3
24-Hydroxycholesterol(d7)	NA	2	392.3	367.3
3-O-SulfoLacCer(d18:1/18:0)	LacCer(d18:1/17:0)	5.2	970.8	548.5
4-beta-Hydroxycholesterol	Cholesterol(d7)	2.14	420.3	385.3
7-keto-Cholesterol	Cholesterol(d7)	3.1	401.3	383.3
CE HETE	CE(18:1(d7))	7.27	706.6	369.2
CE HODE	CE(18:1(d7))	7.32	682.6	369.2
CE HpODE	CE(18:1(d7))	6.76	698.6	369.2
CE oxoHETE	CE(18:1(d7))	6.65	704.6	369.2
CE oxoODE	CE(18:1(d7))	6.73	680.6	369.2
CE(16:1)	CE(18:1(d7))	8.38	640.6	369.3
CE(18:1(d7))	NA	8.43	675.2	369.4
CE(18:1)	CE(18:1(d7))	8.52	668.6	369.3
CE(18:2)	CE(18:1(d7))	8.31	666.6	369.3
CE(20:4)	CE(18:1(d7))	8.19	690.6	369.3
CE(20:5)	CE(18:1(d7))	7.87	688.6	369.3
CE(22:6)	CE(18:1(d7))	7.94	714.6	369.3
Cer(d18:0/16:0)	Cer(d18:1/16:0(d7))	6.3	540.6	284.3
Cer(d18:0/18:0)	Cer(d18:1/16:0(d7))	6.5	568.7	284.3
Cer(d18:0/24:0)	Cer(d18:1/16:0(d7))	7.52	652.9	284.3
Cer(d18:0/24:1)	Cer(d18:1/16:0(d7))	7.2	650.9	284.4
Cer(d18:1/16:0(d7))	NA	5.71	545.5	271.4
Cer(d18:1/16:0)	Cer(d18:1/16:0(d7))	5.68	538.5	264.3
Cer(d18:1/18:0)	Cer(d18:1/16:0(d7))	6.15	566.6	264.3
Cer(d18:1/24:0)	Cer(d18:1/16:0(d7))	7.27	650.6	264.3
Cer(d18:1/24:1)	Cer(d18:1/16:0(d7))	6.85	648.6	264.3
Cholesterol	Cholesterol(d7)	5.18	369.3	369.3
Cholesterol(d7)	NA	5.17	376.2	376.2
Cholesteryl hexoside	CE(18:1(d7))	3.87	566.6	369.3
Coenzyme Q10	TG(15:0/18:1(d7)/15:0)	7.6	863.3	197.2
DG(15:0/18:1(d7))	NA	6.19	605.6	346.5
DG(16:0_18:1)	DG(15:0/18:1(d7))	6.4	612.4	313.3
DG(16:0_20:4)	DG(15:0/18:1(d7))	5.91	634.5	313.3
DG(18:0_18:1)	DG(15:0/18:1(d7))	6.76	640.4	341.3
DG(18:0_20:4)	DG(15:0/18:1(d7))	6.36	662.5	341.3
DG(18:0_22:6)	DG(15:0/18:1(d7))	5.94	686.6	341.3
DG(18:1/18:1)	DG(15:0/18:1(d7))	6.4	638.4	339.3
DG(18:1_20:4)	DG(15:0/18:1(d7))	5.9	660.5	339.3
GB3(d18:1/16:0)	GB3(d18:1/18:0(d3))	5	1025	520.5
GB3(d18:1/18:0(d3))	NA	5.51	1056	551.6
GB3(d18:1/18:0)	GB3(d18:1/18:0(d3))	5.5	1053	548.6
GB3(d18:1/24:0)	GB3(d18:1/18:0(d3))	6.77	1137	632.6
GB3(d18:1/24:1)	GB3(d18:1/18:0(d3))	6.33	1135	630.6
GlcCer(d18:1(d5)/18:0)	NA	5.78	733.6	269.3
HexCer(d18:1/12:0)	GlcCer(d18:1(d5)/18:0)	4.2	644.5	264.3
GlcCer(d18:1/16:0(d3))	NA	5.36	703.7	264.3
Glucosylsphingosine(d5)	NA	1.52	467.2	269.3
HexCer(d18:1/16:0)	GlcCer(d18:1(d5)/18:0)	5.37	700.6	264.3
HexCer(d18:1/18:0)	GlcCer(d18:1(d5)/18:0)	5.77	728.6	264.3
HexCer(d18:1/22:0)	GlcCer(d18:1(d5)/18:0)	6.58	784.7	264.4
HexCer(d18:1/24:0)	GlcCer(d18:1(d5)/18:0)	6.9	812.7	264.3
HexCer(d18:1/24:1)	GlcCer(d18:1(d5)/18:0)	6.54	810.7	264.3
Hexosylsphingosine	Glucosylsphingosine(d5)	1.3	462.3	264.2
LacCer(d18:1/16:0)	GlcCer(d18:1(d5)/18:0)	5.19	862.6	264.3
LacCer(d18:1/17:0)	NA	5.46	876.6	264.3
LacCer(d18:1/18:0)	GlcCer(d18:1(d5)/18:0)	5.68	890.7	264.3
LacCer(d18:1/24:0)	GlcCer(d18:1(d5)/18:0)	6.82	974.8	264.3
LacCer(d18:1/24:1)	GlcCer(d18:1(d5)/18:0)	6.45	972.7	264.3
Lactosylsphingosine	Glucosylsphingosine(d5)	1.4	624.4	264.3
LPC(16:0)	LPC(18:1(d7))	2	496.3	184.1
LPC(16:1)	LPC(18:1(d7))	1.63	494.5	184.1
LPC(18:0)	LPC(18:1(d7))	2.55	524.3	184.1
LPC(18:1(d7))	NA	2.04	529.3	184.1
LPC(18:1)	LPC(18:1(d7))	2.05	522.3	184.1
LPC(20:4)	LPC(18:1(d7))	1.64	544.3	184.1
LPC(22:6)	LPC(18:1(d7))	1.57	568.3	184.1
LPC(24:0)	LPC(18:1(d7))	4.28	608.5	184.1
LPC(24:1)	LPC(18:1(d7))	3.6	606.5	184.1
LPC(26:0)	LPC(18:1(d7))	5.06	636.5	104.1
LPC(26:1)	LPC(18:1(d7))	4.27	634.5	104.1
lyso-GB3	lyso-GB3-d7	1.16	786.6	282.2
lyso-GB3-d7	NA	1.38	793.5	289.3
lyso-GB4	lyso-GB3-d7	1.04	990.6	282.2
MG(16:0)	MG(18:1(d7))	2.48	348.3	239.3
MG(16:1)	MG(18:1(d7))	2	346.3	237.3
MG(18:0)	MG(18:1(d7))	3.04	376.3	267.3
MG(18:1(d7))	NA	2.73	381.3	272.5
MG(18:1)	MG(18:1(d7))	2.74	374.3	265.3
MG(20:4)	MG(18:1(d7))	1.96	396.3	287.3
N-Oleoylethanolamine	LPC(18:1(d7))	2.17	326.3	62.1
N-Palmitoyl-O-phosphocholineserine	LPC(18:1(d7))	1.89	509.5	184.1
Palmitoylethanolamine	LPC(18:1(d7))	2.11	300.3	62.1
PC(15:0/18:1(d7))	NA	5.37	754.6	184.1
PC(16:0/5:0(CHO))	PC(15:0/18:1(d7))	1.95	594.5	184.1
PC(16:0/9:0(CHO))	PC(15:0/18:1(d7))	2.21	650.4	184.1
PC(16:0/9:0(COOH))	PC(15:0/18:1(d7))	2.04	666.4	184.1
PC(18:0/20:4(OH[S]))	PC(15:0/18:1(d7))	4.31	826.6	184.1
PC(18:0/20:4(OOH[S]))	PC(15:0/18:1(d7))	4.24	842.6	184.1
PC(34:1)	PC(15:0/18:1(d7))	5.7	760.6	184.1
PC(36:1)	PC(15:0/18:1(d7))	6.1	788.6	184.1
PC(36:2)	PC(15:0/18:1(d7))	5.69	786.6	184.1
PC(36:4)	PC(15:0/18:1(d7))	5.16	782.6	184.1
PC(38:4)	PC(15:0/18:1(d7))	5.59	810.6	184.1
PC(38:6)	PC(15:0/18:1(d7))	4.92	806.6	184.1
PC(40:5)	PC(15:0/18:1(d7))	5.61	836.6	184.1
PC(40:6)	PC(15:0/18:1(d7))	5.46	834.6	184.1
PC(O-16:0/0:0)	LPC(18:1(d7))	1.77	482.3	184.1
PC(O-16:0/2:0)	LPC(18:1(d7))	1.56	524.3	184.2
PC(O-18:0/2:0)	LPC(18:1(d7))	2.54	552.5	184.1
PE(15:0/18:1(d7))	NA	5.52	711.6	570.5
PE(18:0/20:4(OH[S]))	PE(15:0/18:1(d7))	4.28	784.5	643.4
PE(18:0/20:4(OOH[S]))	PE(15:0/18:1(d7))	4.23	800.5	659.4
PE(34:1)	PE(15:0/18:1(d7))	5.8	718.6	577.6
PE(36:1)	PE(15:0/18:1(d7))	6.22	746.6	605.5
PE(36:2)	PE(15:0/18:1(d7))	5.85	744.6	603.5
PE(36:4)	PE(15:0/18:1(d7))	5.3	740.6	599.5
PE(38:4)	PE(15:0/18:1(d7))	5.77	768.6	627.5
PE(38:5)	PE(15:0/18:1(d7))	5.31	766.6	625.5
PE(38:6)	PE(15:0/18:1(d7))	5.07	764.6	623.5
PE(40:4)	PE(15:0/18:1(d7))	6.01	796.6	655.5
PE(40:5)	PE(15:0/18:1(d7))	5.8	794.6	635.5
PE(40:6)	PE(15:0/18:1(d7))	5.59	792.6	651.5
PE(40:7)	PE(15:0/18:1(d7))	5.15	790.6	649.5
Sitosteryl hexoside	CE(18:1(d7))	4.45	594.6	397.4
SM(d18:1(d9)/18:1)	NA	5.24	738.7	184.1
SM(d18:1/16:0)	SM(d18:1(d9)/18:1)	5.22	703.6	184.1
SM(d18:1/18:0)	SM(d18:1(d9)/18:1)	5.74	731.6	184.1
SM(d18:1/24:0)	SM(d18:1(d9)/18:1)	6.9	815.7	184.1
SM(d18:1/24:1)	SM(d18:1(d9)/18:1)	6.5	813.7	184.1
Sphinganine	Sphingosine(d17:1)	1.88	302.2	284.3
Sphinganine 1-phosphate	Sphingosine 1-phosphate-d7	1.68	382.3	284.3
Sphingosine	Sphingosine(d17:1)	1.68	300.2	264.3
Sphingosine 1-phosphate	Sphingosine 1-phosphate-d7	1.45	380.3	264.3
Sphingosine 1-phosphate-d7	NA	1.53	387.3	271.3
Sphingosine 1-phosphocholine	LPC(18:1(d7))	1.12	465.5	184.1
Sphingosine(d17:1)	NA	1.52	286.2	250.3
TG(15:0/18:1(d7)/15:0)	NA	8.15	829.8	570.8
TG(18:0_36:2)	TG(15:0/18:1(d7)/15:0)	8.55	904.7	603.4
TG(18:1_34:2)	TG(15:0/18:1(d7)/15:0)	8.14	874.7	575.4
TG(18:1_34:3)	TG(15:0/18:1(d7)/15:0)	7.95	872.7	573.4
TG(20:4_32:1)	TG(15:0/18:1(d7)/15:0)	7.78	870.6	549.3
TG(20:4_34:2)	TG(15:0/18:1(d7)/15:0)	7.94	896.6	575.3
TG(20:4_34:3)	TG(15:0/18:1(d7)/15:0)	7.68	894.6	573.3
TG(20:4_36:0)	TG(15:0/18:1(d7)/15:0)	8.18	928.8	607.5
TG(20:4_36:2)	TG(15:0/18:1(d7)/15:0)	7.95	924.7	603.4
TG(20:4_36:3)	TG(15:0/18:1(d7)/15:0)	7.9	922.7	601.4
TG(22:6_36:2)	TG(15:0/18:1(d7)/15:0)	7.86	948.7	603.4
TG(22:6_38:1)	TG(15:0/18:1(d7)/15:0)	8.29	978.7	633.4
TG(22:6_38:2)	TG(15:0/18:1(d7)/15:0)	8.1	976.7	631.4
METABOLITES_END
#END