#METABOLOMICS WORKBENCH DiegoC_20241001_130811 DATATRACK_ID:5254 STUDY_ID:ST003515 ANALYSIS_ID:AN005772 PROJECT_ID:PR002160
VERSION                          	1
CREATED_ON                       	10-10-2024
#PROJECT
PR:PROJECT_TITLE                 	Untargeted Metabolomics of 3xTg-AD Neurotoxic Astrocytes
PR:PROJECT_SUMMARY               	Alzheimer's disease (AD) is the most common form of dementia, affecting
PR:PROJECT_SUMMARY               	approximately 47M people worldwide. Histological features and genetic risk
PR:PROJECT_SUMMARY               	factors, among other evidence, supported the amyloid hypothesis of the disease.
PR:PROJECT_SUMMARY               	This neuronocentric paradigm is currently undergoing a shift, considering
PR:PROJECT_SUMMARY               	evidence of the role of other cell types, such as microglia and astrocytes, in
PR:PROJECT_SUMMARY               	disease progression. Previously, we described a particular astrocyte subtype
PR:PROJECT_SUMMARY               	obtained from the 3xTg-AD murine model that displays neurotoxic properties in
PR:PROJECT_SUMMARY               	vitro. We continue here our exploratory analysis through the lens of
PR:PROJECT_SUMMARY               	metabolomics to identify potentially altered metabolites and biological
PR:PROJECT_SUMMARY               	pathways. Cell extracts from neurotoxic and control astrocytes were compared
PR:PROJECT_SUMMARY               	using HRMS-based metabolomics. Around 12% of metabolic features demonstrated
PR:PROJECT_SUMMARY               	significant differences between neurotoxic and control astrocytes, including
PR:PROJECT_SUMMARY               	alterations in the key metabolite glutamate. Consistent with our previous
PR:PROJECT_SUMMARY               	transcriptomic study, the present results illustrate many homeostatic and
PR:PROJECT_SUMMARY               	regulatory functions of metabolites, suggesting that neurotoxic 3xTg-AD
PR:PROJECT_SUMMARY               	astrocytes exhibit alterations in the Krebs cycle as well as the prostaglandin
PR:PROJECT_SUMMARY               	pathway. This is the first metabolomic study performed in 3xTg-AD neurotoxic
PR:PROJECT_SUMMARY               	astrocytes. These results provide insight into metabolic alterations potentially
PR:PROJECT_SUMMARY               	associated with neurotoxicity and pathology progression in the 3xTg-AD mouse
PR:PROJECT_SUMMARY               	model and strengthen the therapeutic potential of astrocytes in AD.
PR:INSTITUTE                     	Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)
PR:DEPARTMENT                    	Neuroquímica
PR:LAST_NAME                     	Carvalho
PR:FIRST_NAME                    	Diego
PR:ADDRESS                       	Isidoro de María 1614, Montevideo, Montevideo, 11800, Uruguay
PR:EMAIL                         	dicarez@fq.edu.uy
PR:PHONE                         	(+598) 2924 1879
PR:DOI                           	http://dx.doi.org/10.21228/M8DR6G
#STUDY
ST:STUDY_TITLE                   	Untargeted Metabolomics of 3xTg-AD Neurotoxic Astrocytes
ST:STUDY_SUMMARY                 	Alzheimer's disease (AD) is the most common form of dementia, affecting
ST:STUDY_SUMMARY                 	approximately 47M people worldwide. Histological features and genetic risk
ST:STUDY_SUMMARY                 	factors, among other evidence, supported the amyloid hypothesis of the disease.
ST:STUDY_SUMMARY                 	This neuronocentric paradigm is currently undergoing a shift, considering
ST:STUDY_SUMMARY                 	evidence of the role of other cell types, such as microglia and astrocytes, in
ST:STUDY_SUMMARY                 	disease progression. Previously, we described a particular astrocyte subtype
ST:STUDY_SUMMARY                 	obtained from the 3xTg-AD murine model that displays neurotoxic properties in
ST:STUDY_SUMMARY                 	vitro. We continue here our exploratory analysis through the lens of
ST:STUDY_SUMMARY                 	metabolomics to identify potentially altered metabolites and biological
ST:STUDY_SUMMARY                 	pathways. Cell extracts from neurotoxic and control astrocytes were compared
ST:STUDY_SUMMARY                 	using HRMS-based metabolomics. Around 12% of metabolic features demonstrated
ST:STUDY_SUMMARY                 	significant differences between neurotoxic and control astrocytes, including
ST:STUDY_SUMMARY                 	alterations in the key metabolite glutamate. Consistent with our previous
ST:STUDY_SUMMARY                 	transcriptomic study, the present results illustrate many homeostatic and
ST:STUDY_SUMMARY                 	regulatory functions of metabolites, suggesting that neurotoxic 3xTg-AD
ST:STUDY_SUMMARY                 	astrocytes exhibit alterations in the Krebs cycle as well as the prostaglandin
ST:STUDY_SUMMARY                 	pathway. This is the first metabolomic study performed in 3xTg-AD neurotoxic
ST:STUDY_SUMMARY                 	astrocytes. These results provide insight into metabolic alterations potentially
ST:STUDY_SUMMARY                 	associated with neurotoxicity and pathology progression in the 3xTg-AD mouse
ST:STUDY_SUMMARY                 	model and strengthen the therapeutic potential of astrocytes in AD.
ST:INSTITUTE                     	Instituto de Investigaciones Biológicas Clemente Estable (IIBCE)
ST:LAST_NAME                     	Carvalho
ST:FIRST_NAME                    	Diego
ST:ADDRESS                       	Isidoro de María 1614, Montevideo, Montevideo, 11800, Uruguay
ST:EMAIL                         	dicarez@fq.edu.uy
ST:PHONE                         	(+598) 2924 1879
ST:SUBMIT_DATE                   	2024-10-01
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENDER                        	Female
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	2A	2A_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_139.raw
SUBJECT_SAMPLE_FACTORS           	2A	2A_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_140.raw
SUBJECT_SAMPLE_FACTORS           	2A	2A_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_141.raw
SUBJECT_SAMPLE_FACTORS           	2A	2A_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_142.raw
SUBJECT_SAMPLE_FACTORS           	2A	2A_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_143.raw
SUBJECT_SAMPLE_FACTORS           	2A	2A_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_144.raw
SUBJECT_SAMPLE_FACTORS           	2B	2B_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_019.raw
SUBJECT_SAMPLE_FACTORS           	2B	2B_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_020.raw
SUBJECT_SAMPLE_FACTORS           	2B	2B_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_021.raw
SUBJECT_SAMPLE_FACTORS           	2B	2B_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_022.raw
SUBJECT_SAMPLE_FACTORS           	2B	2B_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_023.raw
SUBJECT_SAMPLE_FACTORS           	2B	2B_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_024.raw
SUBJECT_SAMPLE_FACTORS           	2C	2C_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_037.raw
SUBJECT_SAMPLE_FACTORS           	2C	2C_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_038.raw
SUBJECT_SAMPLE_FACTORS           	2C	2C_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_039.raw
SUBJECT_SAMPLE_FACTORS           	2C	2C_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_040.raw
SUBJECT_SAMPLE_FACTORS           	2C	2C_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_041.raw
SUBJECT_SAMPLE_FACTORS           	2C	2C_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_042.raw
SUBJECT_SAMPLE_FACTORS           	5A	5A_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_289.raw
SUBJECT_SAMPLE_FACTORS           	5A	5A_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_290.raw
SUBJECT_SAMPLE_FACTORS           	5A	5A_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_291.raw
SUBJECT_SAMPLE_FACTORS           	5A	5A_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_292.raw
SUBJECT_SAMPLE_FACTORS           	5A	5A_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_293.raw
SUBJECT_SAMPLE_FACTORS           	5A	5A_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_294.raw
SUBJECT_SAMPLE_FACTORS           	5B	5B_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_265.raw
SUBJECT_SAMPLE_FACTORS           	5B	5B_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_266.raw
SUBJECT_SAMPLE_FACTORS           	5B	5B_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_267.raw
SUBJECT_SAMPLE_FACTORS           	5B	5B_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_268.raw
SUBJECT_SAMPLE_FACTORS           	5B	5B_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_269.raw
SUBJECT_SAMPLE_FACTORS           	5B	5B_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_270.raw
SUBJECT_SAMPLE_FACTORS           	5C	5C_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_301.raw
SUBJECT_SAMPLE_FACTORS           	5C	5C_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_302.raw
SUBJECT_SAMPLE_FACTORS           	5C	5C_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_303.raw
SUBJECT_SAMPLE_FACTORS           	5C	5C_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_304.raw
SUBJECT_SAMPLE_FACTORS           	5C	5C_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_305.raw
SUBJECT_SAMPLE_FACTORS           	5C	5C_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_306.raw
SUBJECT_SAMPLE_FACTORS           	9A	9A_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_025.raw
SUBJECT_SAMPLE_FACTORS           	9A	9A_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_026.raw
SUBJECT_SAMPLE_FACTORS           	9A	9A_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_027.raw
SUBJECT_SAMPLE_FACTORS           	9A	9A_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_028.raw
SUBJECT_SAMPLE_FACTORS           	9A	9A_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_029.raw
SUBJECT_SAMPLE_FACTORS           	9A	9A_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_030.raw
SUBJECT_SAMPLE_FACTORS           	9B	9B_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_127.raw
SUBJECT_SAMPLE_FACTORS           	9B	9B_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_128.raw
SUBJECT_SAMPLE_FACTORS           	9B	9B_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_129.raw
SUBJECT_SAMPLE_FACTORS           	9B	9B_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_130.raw
SUBJECT_SAMPLE_FACTORS           	9B	9B_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_131.raw
SUBJECT_SAMPLE_FACTORS           	9B	9B_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_132.raw
SUBJECT_SAMPLE_FACTORS           	9C	9C_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_133.raw
SUBJECT_SAMPLE_FACTORS           	9C	9C_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_134.raw
SUBJECT_SAMPLE_FACTORS           	9C	9C_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_135.raw
SUBJECT_SAMPLE_FACTORS           	9C	9C_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_136.raw
SUBJECT_SAMPLE_FACTORS           	9C	9C_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_137.raw
SUBJECT_SAMPLE_FACTORS           	9C	9C_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_138.raw
SUBJECT_SAMPLE_FACTORS           	3A	3A_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_187.raw
SUBJECT_SAMPLE_FACTORS           	3A	3A_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_188.raw
SUBJECT_SAMPLE_FACTORS           	3A	3A_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_189.raw
SUBJECT_SAMPLE_FACTORS           	3A	3A_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_190.raw
SUBJECT_SAMPLE_FACTORS           	3A	3A_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_191.raw
SUBJECT_SAMPLE_FACTORS           	3A	3A_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_192.raw
SUBJECT_SAMPLE_FACTORS           	3B	3B_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_169.raw
SUBJECT_SAMPLE_FACTORS           	3B	3B_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_170.raw
SUBJECT_SAMPLE_FACTORS           	3B	3B_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_171.raw
SUBJECT_SAMPLE_FACTORS           	3B	3B_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_172.raw
SUBJECT_SAMPLE_FACTORS           	3B	3B_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_173.raw
SUBJECT_SAMPLE_FACTORS           	3B	3B_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_174.raw
SUBJECT_SAMPLE_FACTORS           	3C	3C_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_247.raw
SUBJECT_SAMPLE_FACTORS           	3C	3C_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_248.raw
SUBJECT_SAMPLE_FACTORS           	3C	3C_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_249.raw
SUBJECT_SAMPLE_FACTORS           	3C	3C_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_250.raw
SUBJECT_SAMPLE_FACTORS           	3C	3C_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_251.raw
SUBJECT_SAMPLE_FACTORS           	3C	3C_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_252.raw
SUBJECT_SAMPLE_FACTORS           	4A	4A_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_283.raw
SUBJECT_SAMPLE_FACTORS           	4A	4A_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_284.raw
SUBJECT_SAMPLE_FACTORS           	4A	4A_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_285.raw
SUBJECT_SAMPLE_FACTORS           	4A	4A_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_286.raw
SUBJECT_SAMPLE_FACTORS           	4A	4A_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_287.raw
SUBJECT_SAMPLE_FACTORS           	4A	4A_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_288.raw
SUBJECT_SAMPLE_FACTORS           	4B	4B_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_307.raw
SUBJECT_SAMPLE_FACTORS           	4B	4B_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_308.raw
SUBJECT_SAMPLE_FACTORS           	4B	4B_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_309.raw
SUBJECT_SAMPLE_FACTORS           	4B	4B_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_310.raw
SUBJECT_SAMPLE_FACTORS           	4B	4B_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_311.raw
SUBJECT_SAMPLE_FACTORS           	4B	4B_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_312.raw
SUBJECT_SAMPLE_FACTORS           	4C	4C_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_325.raw
SUBJECT_SAMPLE_FACTORS           	4C	4C_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_326.raw
SUBJECT_SAMPLE_FACTORS           	4C	4C_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_327.raw
SUBJECT_SAMPLE_FACTORS           	4C	4C_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_328.raw
SUBJECT_SAMPLE_FACTORS           	4C	4C_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_329.raw
SUBJECT_SAMPLE_FACTORS           	4C	4C_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_330.raw
SUBJECT_SAMPLE_FACTORS           	7A	7A_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_073.raw
SUBJECT_SAMPLE_FACTORS           	7A	7A_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_074.raw
SUBJECT_SAMPLE_FACTORS           	7A	7A_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_075.raw
SUBJECT_SAMPLE_FACTORS           	7A	7A_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_076.raw
SUBJECT_SAMPLE_FACTORS           	7A	7A_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_077.raw
SUBJECT_SAMPLE_FACTORS           	7A	7A_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_078.raw
SUBJECT_SAMPLE_FACTORS           	7B	7B_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_067.raw
SUBJECT_SAMPLE_FACTORS           	7B	7B_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_068.raw
SUBJECT_SAMPLE_FACTORS           	7B	7B_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_069.raw
SUBJECT_SAMPLE_FACTORS           	7B	7B_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_070.raw
SUBJECT_SAMPLE_FACTORS           	7B	7B_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_071.raw
SUBJECT_SAMPLE_FACTORS           	7B	7B_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_072.raw
SUBJECT_SAMPLE_FACTORS           	7C	7C_1	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_043.raw
SUBJECT_SAMPLE_FACTORS           	7C	7C_2	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_044.raw
SUBJECT_SAMPLE_FACTORS           	7C	7C_3	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_045.raw
SUBJECT_SAMPLE_FACTORS           	7C	7C_4	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_046.raw
SUBJECT_SAMPLE_FACTORS           	7C	7C_5	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_047.raw
SUBJECT_SAMPLE_FACTORS           	7C	7C_6	Sample source:Cultured_astrocytes | Genotype:3xTg-AD | Condition:Old	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_048.raw
SUBJECT_SAMPLE_FACTORS           	1A	1A_1	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_091.raw
SUBJECT_SAMPLE_FACTORS           	1A	1A_2	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_092.raw
SUBJECT_SAMPLE_FACTORS           	1A	1A_3	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_093.raw
SUBJECT_SAMPLE_FACTORS           	1A	1A_4	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_094.raw
SUBJECT_SAMPLE_FACTORS           	1A	1A_5	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_095.raw
SUBJECT_SAMPLE_FACTORS           	1A	1A_6	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_096.raw
SUBJECT_SAMPLE_FACTORS           	1B	1B_1	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_211.raw
SUBJECT_SAMPLE_FACTORS           	1B	1B_2	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_212.raw
SUBJECT_SAMPLE_FACTORS           	1B	1B_3	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_213.raw
SUBJECT_SAMPLE_FACTORS           	1B	1B_4	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_214.raw
SUBJECT_SAMPLE_FACTORS           	1B	1B_5	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_215.raw
SUBJECT_SAMPLE_FACTORS           	1B	1B_6	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_216.raw
SUBJECT_SAMPLE_FACTORS           	1C	1C_1	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_055.raw
SUBJECT_SAMPLE_FACTORS           	1C	1C_2	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_056.raw
SUBJECT_SAMPLE_FACTORS           	1C	1C_3	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_057.raw
SUBJECT_SAMPLE_FACTORS           	1C	1C_4	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_058.raw
SUBJECT_SAMPLE_FACTORS           	1C	1C_5	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_059.raw
SUBJECT_SAMPLE_FACTORS           	1C	1C_6	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_060.raw
SUBJECT_SAMPLE_FACTORS           	6A	6A_1	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_253.raw
SUBJECT_SAMPLE_FACTORS           	6A	6A_2	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_254.raw
SUBJECT_SAMPLE_FACTORS           	6A	6A_3	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_255.raw
SUBJECT_SAMPLE_FACTORS           	6A	6A_4	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_256.raw
SUBJECT_SAMPLE_FACTORS           	6A	6A_5	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_257.raw
SUBJECT_SAMPLE_FACTORS           	6A	6A_6	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_258.raw
SUBJECT_SAMPLE_FACTORS           	6B	6B_1	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_115.raw
SUBJECT_SAMPLE_FACTORS           	6B	6B_2	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_116.raw
SUBJECT_SAMPLE_FACTORS           	6B	6B_3	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_117.raw
SUBJECT_SAMPLE_FACTORS           	6B	6B_4	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_118.raw
SUBJECT_SAMPLE_FACTORS           	6B	6B_5	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_119.raw
SUBJECT_SAMPLE_FACTORS           	6B	6B_6	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_120.raw
SUBJECT_SAMPLE_FACTORS           	6C	6C_1	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_085.raw
SUBJECT_SAMPLE_FACTORS           	6C	6C_2	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_086.raw
SUBJECT_SAMPLE_FACTORS           	6C	6C_3	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_087.raw
SUBJECT_SAMPLE_FACTORS           	6C	6C_4	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_088.raw
SUBJECT_SAMPLE_FACTORS           	6C	6C_5	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_089.raw
SUBJECT_SAMPLE_FACTORS           	6C	6C_6	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_090.raw
SUBJECT_SAMPLE_FACTORS           	8A	8A_1	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_205.raw
SUBJECT_SAMPLE_FACTORS           	8A	8A_2	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_206.raw
SUBJECT_SAMPLE_FACTORS           	8A	8A_3	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_207.raw
SUBJECT_SAMPLE_FACTORS           	8A	8A_4	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_208.raw
SUBJECT_SAMPLE_FACTORS           	8A	8A_5	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_209.raw
SUBJECT_SAMPLE_FACTORS           	8A	8A_6	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_210.raw
SUBJECT_SAMPLE_FACTORS           	8B	8B_1	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_109.raw
SUBJECT_SAMPLE_FACTORS           	8B	8B_2	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_110.raw
SUBJECT_SAMPLE_FACTORS           	8B	8B_3	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_111.raw
SUBJECT_SAMPLE_FACTORS           	8B	8B_4	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_112.raw
SUBJECT_SAMPLE_FACTORS           	8B	8B_5	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_113.raw
SUBJECT_SAMPLE_FACTORS           	8B	8B_6	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_114.raw
SUBJECT_SAMPLE_FACTORS           	8C	8C_1	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_049.raw
SUBJECT_SAMPLE_FACTORS           	8C	8C_2	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_050.raw
SUBJECT_SAMPLE_FACTORS           	8C	8C_3	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_051.raw
SUBJECT_SAMPLE_FACTORS           	8C	8C_4	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_052.raw
SUBJECT_SAMPLE_FACTORS           	8C	8C_5	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_053.raw
SUBJECT_SAMPLE_FACTORS           	8C	8C_6	Sample source:Cultured_astrocytes | Genotype:Wild-type | Condition:Neonatal	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_054.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1a_1	q3June2014_1a_1	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_007.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1a_2	q3June2014_1a_2	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_008.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1a_3	q3June2014_1a_3	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_009.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1a_4	q3June2014_1a_4	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_010.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1a_5	q3June2014_1a_5	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_011.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1a_6	q3June2014_1a_6	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_012.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1b_1	q3June2014_1b_1	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_013.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1b_2	q3June2014_1b_2	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_014.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1b_3	q3June2014_1b_3	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_015.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1b_4	q3June2014_1b_4	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_016.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1b_5	q3June2014_1b_5	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_017.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1b_6	q3June2014_1b_6	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_018.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1c_1	q3June2014_1c_1	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_193.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1c_2	q3June2014_1c_2	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_194.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1c_3	q3June2014_1c_3	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_195.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1c_4	q3June2014_1c_4	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_196.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1c_5	q3June2014_1c_5	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_197.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1c_6	q3June2014_1c_6	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_198.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1d_1	q3June2014_1d_1	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_199.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1d_2	q3June2014_1d_2	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_200.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1d_3	q3June2014_1d_3	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_201.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1d_4	q3June2014_1d_4	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_202.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1d_5	q3June2014_1d_5	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_203.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1d_6	q3June2014_1d_6	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_204.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1e_1	q3June2014_1e_1	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_373.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1e_2	q3June2014_1e_2	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_374.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1e_3	q3June2014_1e_3	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_375.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1e_4	q3June2014_1e_4	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_376.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1e_5	q3June2014_1e_5	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_377.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1e_6	q3June2014_1e_6	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_378.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1f_1	q3June2014_1f_1	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_379.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1f_2	q3June2014_1f_2	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_380.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1f_3	q3June2014_1f_3	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_381.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1f_4	q3June2014_1f_4	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_382.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1f_5	q3June2014_1f_5	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_383.raw
SUBJECT_SAMPLE_FACTORS           	q3June2014_1f_6	q3June2014_1f_6	Sample source:Pooled_human_plasma | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_384.raw
SUBJECT_SAMPLE_FACTORS           	nist_1	nist_1	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_001.raw
SUBJECT_SAMPLE_FACTORS           	nist_2	nist_2	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_002.raw
SUBJECT_SAMPLE_FACTORS           	nist2_1	nist2_1	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_385.raw
SUBJECT_SAMPLE_FACTORS           	nist2_2	nist2_2	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_386.raw
SUBJECT_SAMPLE_FACTORS           	nist2_3	nist2_3	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_387.raw
SUBJECT_SAMPLE_FACTORS           	nist2_4	nist2_4	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_388.raw
SUBJECT_SAMPLE_FACTORS           	nist2_5	nist2_5	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_389.raw
SUBJECT_SAMPLE_FACTORS           	nist2_6	nist2_6	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_390.raw
SUBJECT_SAMPLE_FACTORS           	nist_3	nist_3	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_003.raw
SUBJECT_SAMPLE_FACTORS           	nist_4	nist_4	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_004.raw
SUBJECT_SAMPLE_FACTORS           	nist_5	nist_5	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=HILIC_Positive; RAW_FILE_NAME=VT_190205_005.raw
SUBJECT_SAMPLE_FACTORS           	nist_6	nist_6	Sample source:Reference_standards | Genotype:- | Condition:Quality_control	Column_Polarity=C18_Negative; RAW_FILE_NAME=VT_190205_006.raw
#COLLECTION
CO:COLLECTION_SUMMARY            	Neurotoxic astrocyte cultures were prepared from the cerebral cortex and
CO:COLLECTION_SUMMARY            	hippocampus of 9- to 10-month-old 3xTg-AD female mice according to previously
CO:COLLECTION_SUMMARY            	described methods [2]. Female animals were used since sex differences in the
CO:COLLECTION_SUMMARY            	development of the pathology have been described in this AD murine model, with a
CO:COLLECTION_SUMMARY            	greater expression of transgenes in females [3,4]. Astrocytes isolated from 9-
CO:COLLECTION_SUMMARY            	to 10-month-old non-Tg mice were not used as controls in our studies due to the
CO:COLLECTION_SUMMARY            	low yield achieved [2]. Instead, astrocytic cultures derived from neonatal
CO:COLLECTION_SUMMARY            	non-Tg mice as well as astrocytes from neonatal 3xTg-AD mice were used as
CO:COLLECTION_SUMMARY            	controls. These non-toxic controls allowed us to assess whether astrocytes from
CO:COLLECTION_SUMMARY            	this mice model of AD exhibit alterations when isolated before the disease
CO:COLLECTION_SUMMARY            	onset. Nontoxic control astrocyte cultures were derived from the cerebral cortex
CO:COLLECTION_SUMMARY            	and hippocampus of neonatal (postnatal day 0–2) 3xTg-AD and non-Tg (C57BL/6J)
CO:COLLECTION_SUMMARY            	mice following the methods described by Cassina et al. with minor modifications
CO:COLLECTION_SUMMARY            	[2,5]. All cell cultures were amplified and maintained at 37°C in a humidified
CO:COLLECTION_SUMMARY            	incubator with 5% CO2. References [2] P. Diaz-Amarilla, F. Arredondo, R.
CO:COLLECTION_SUMMARY            	Dapueto, V. Boix, D. Carvalho, M.D. Santi, E. Vasilskis, R. Mesquita-Ribeiro, F.
CO:COLLECTION_SUMMARY            	Dajas-Bailador, J.A. Abin-Carriquiry, H. Engler, E. Savio, Isolation and
CO:COLLECTION_SUMMARY            	characterization of neurotoxic astrocytes derived from old triple transgenic
CO:COLLECTION_SUMMARY            	Alzheimer’s disease mice, Neurochem. Int. 159 (2022).
CO:COLLECTION_SUMMARY            	https://doi.org/10.1016/j.neuint.2022.105403. [3] J.C. Carroll, E.R. Rosario, S.
CO:COLLECTION_SUMMARY            	Kreimer, A. Villamagna, E. Gentzschein, F.Z. Stanczyk, C.J. Pike, Sex
CO:COLLECTION_SUMMARY            	differences in β-amyloid accumulation in 3xTg-AD mice: Role of neonatal sex
CO:COLLECTION_SUMMARY            	steroid hormone exposure, Brain Res. 1366 (2010) 233–245.
CO:COLLECTION_SUMMARY            	https://doi.org/10.1016/J.BRAINRES.2010.10.009. [4] L.K. Clinton, L.M. Billings,
CO:COLLECTION_SUMMARY            	K.N. Green, A. Caccamo, J. Ngo, S. Oddo, J.L. McGaugh, F.M. LaFerla,
CO:COLLECTION_SUMMARY            	Age-dependent sexual dimorphism in cognition and stress response in the 3xTg-AD
CO:COLLECTION_SUMMARY            	mice, Neurobiol. Dis. 28 (2007) 76–82.
CO:COLLECTION_SUMMARY            	https://doi.org/10.1016/J.NBD.2007.06.013. [5] P. Cassina, H. Peluffo, M. Pehar,
CO:COLLECTION_SUMMARY            	L. Martinez-Palma, A. Ressia, J.S. Beckman, A.G. Estévez, L. Barbeito,
CO:COLLECTION_SUMMARY            	Peroxynitrite triggers a phenotypic transformation in spinal cord astrocytes
CO:COLLECTION_SUMMARY            	that induces motor neuron apoptosis, J. Neurosci. Res. 67 (2002) 21–29.
CO:COLLECTION_SUMMARY            	https://doi.org/10.1002/jnr.10107.
CO:SAMPLE_TYPE                   	Astrocytes
#TREATMENT
TR:TREATMENT_SUMMARY             	No treatment
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Extraction of metabolites from cultured cells was performed based on previous
SP:SAMPLEPREP_SUMMARY            	work by Go et al. (2015), Liu et al. (2019), as well as Sapcariu et al. (2014)
SP:SAMPLEPREP_SUMMARY            	[6-8]. One sample consisted of metabolites extracted and pooled from 3 wells of
SP:SAMPLEPREP_SUMMARY            	a 6-well plate, pooling a total of 9 samples per condition. Briefly, confluent
SP:SAMPLEPREP_SUMMARY            	cells were washed with 0.9% (m/v) NaCl at room temperature, followed by the
SP:SAMPLEPREP_SUMMARY            	addition of 300 µL of an ice-cold HPLC-grade acetonitrile-water solution
SP:SAMPLEPREP_SUMMARY            	mixture (1:2) per well. This allowed us to scrape cells, precipitate proteins,
SP:SAMPLEPREP_SUMMARY            	and extract metabolites. The extracts were kept at 4 °C for 30 min. and
SP:SAMPLEPREP_SUMMARY            	centrifuged at 16,100 g for 10 min to remove the protein and any remaining
SP:SAMPLEPREP_SUMMARY            	insoluble fraction. Supernatants were transferred to screw-cap vials and dried
SP:SAMPLEPREP_SUMMARY            	in a fast vacuum centrifuge (overnight, 35°C).
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Solvent A: Water; Solvent B: Acetonitrile; Solvent C: 2% formic acid (v/v) in
CH:CHROMATOGRAPHY_SUMMARY        	water
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000
CH:COLUMN_NAME                   	Thermo Accucore HILIC (50 x 2.1mm,2.6um)
CH:COLUMN_TEMPERATURE            	60
CH:FLOW_GRADIENT                 	Mobile phase conditions consisted of 22.5% A, 75% B, 2.5% C which was held for
CH:FLOW_GRADIENT                 	1.5 min, with a linear gradient to 77.5% A, 20% B, 2.5% C at 4 min, and held for
CH:FLOW_GRADIENT                 	1 min
CH:FLOW_RATE                     	Flow rate of the HILIC column was maintained at 0.35 mL/min until 1.5 min,
CH:FLOW_RATE                     	increased to 0.4 mL/min at 4 min and held for 1 min, resulting in a total
CH:FLOW_RATE                     	analytical run time of 5 min
CH:SOLVENT_A                     	100% water
CH:SOLVENT_B                     	100% acetonitrile
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:SOLVENT_C                     	98% water/2% formic acid
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:MS_COMMENTS                   	MS acquisition Comments: Collected mass spectral data were analyzed for features
MS:MS_COMMENTS                   	using Xcaliber (ThermoFisher; Waltham MA). Ultra-high resolution mass
MS:MS_COMMENTS                   	spectrometry operating at 60,000 and 120,000 resolution has previously been
MS:MS_COMMENTS                   	shown to provide effective metabolite quantification in biological extracts [11]
MS:MS_COMMENTS                   	and the use of complementary chromatography and ionization phases have been
MS:MS_COMMENTS                   	shown to improve the detection of endogenous and exogenous chemicals. Data
MS:MS_COMMENTS                   	processing Comments: Raw mass spectral data files were converted to computable
MS:MS_COMMENTS                   	document format (CDF) using Xcalibur file conversion software (Thermo Fisher
MS:MS_COMMENTS                   	Scientific, San Diego, CA) for further processing. Software/procedures used for
MS:MS_COMMENTS                   	feature assignments: Data were processed for peak extraction, noise filtering,
MS:MS_COMMENTS                   	m/z and retention time alignment, and quantification of ion intensities using
MS:MS_COMMENTS                   	apLCMS [29] with enhanced data extraction using xMSanalyzer [30]. xMSanalyzer
MS:MS_COMMENTS                   	improves feature detection through systematic data re-extraction, statistical
MS:MS_COMMENTS                   	filtering and fusion [30]. Metabolite feature peak intensity values ​​were
MS:MS_COMMENTS                   	summarized by the median in triplicate (technical replicates) and subjected to
MS:MS_COMMENTS                   	quality assessment. The samples were filtered considering an overall Pearson
MS:MS_COMMENTS                   	correlation of technical replicates of (r) > 0.70 and a cut-off coefficient
MS:MS_COMMENTS                   	of variation of 75%. Considering the number of missing values, one sample
MS:MS_COMMENTS                   	outlier was further removed from the C18 and HILIC cell extraction feature
MS:MS_COMMENTS                   	tables. Attached is the xMSAnalyzer table of the detected peak intensities. As
MS:MS_COMMENTS                   	stated above, the values ​​represent the median of three technical
MS:MS_COMMENTS                   	replicates evaluated per original sample. For example, samples labeled 2A_1,
MS:MS_COMMENTS                   	2A_3 and 2A_5 in the design table are summarized under label 2A in the features
MS:MS_COMMENTS                   	table. For further details on the procedure, please refer to the xMSAnalyzer
MS:MS_COMMENTS                   	publication. Uppal, K., Soltow, Q.A., Strobel, F.H. et al. xMSanalyzer:
MS:MS_COMMENTS                   	automated pipeline for improved feature detection and downstream analysis of
MS:MS_COMMENTS                   	large-scale, non-targeted metabolomics data. BMC Bioinformatics 14, 15 (2013).
MS:MS_COMMENTS                   	https://doi.org/10.1186/1471-2105-14-15
MS:ION_MODE                      	POSITIVE
MS:MS_RESULTS_FILE               	ST003515_AN005772_Results.txt	UNITS:m/z	Has m/z:Yes	Has RT:Yes	RT units:Seconds
#END