#METABOLOMICS WORKBENCH ZHULABMAYO_20241011_104302 DATATRACK_ID:5287 STUDY_ID:ST003519 ANALYSIS_ID:AN005777 PROJECT_ID:PR002164 VERSION 1 CREATED_ON October 12, 2024, 11:15 am #PROJECT PR:PROJECT_TITLE Rattus norvegicus PR:PROJECT_SUMMARY Rat hearts were isolated for ex vivo Langendorff perfusion. After the treatments PR:PROJECT_SUMMARY were applied, right atrial tissue was collected and frozen at -80°C until IC-MS PR:PROJECT_SUMMARY analysis PR:INSTITUTE Mayo Clinic PR:LAST_NAME Zhu PR:FIRST_NAME Wuqiang PR:ADDRESS 5951 E Mayo Blvd, RE 1-408, Zhu Lab, Phoenix, AZ 85054 PR:EMAIL zhu.wuqiang@mayo.edu PR:PHONE 480-301-9693 #STUDY ST:STUDY_TITLE Effect of insluin on metabolism of ex vivo ischemia heart ST:STUDY_SUMMARY Ex vivo hearts were pre-perfused with or without insulin, followed by ischemia ST:STUDY_SUMMARY induced by the cessation of perfusion. After 25 minutes of ischemia, hearts ST:STUDY_SUMMARY underwent 30 minutes of reperfusion. Right atrial tissue was then collected for ST:STUDY_SUMMARY IC-MS analysis. Three groups were included: a control group without ischemia, a ST:STUDY_SUMMARY group with 25 minutes of ischemia followed by reperfusion, and a group ST:STUDY_SUMMARY pre-perfused with insulin for 30 minutes prior to ischemia and reperfusion. ST:INSTITUTE Mayo Clinic ST:LAST_NAME Qiu ST:FIRST_NAME Huiliang ST:ADDRESS 5951 E Mayo Blvd, RE 1-408, Zhu Lab, Phoenix, AZ 85054 ST:EMAIL Qiu.Huiliang@mayo.edu ST:PHONE 415-629-7041 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Rattus norvegicus SU:TAXONOMY_ID 10116 SU:GENOTYPE_STRAIN WT SU:GENDER Male and female #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS Control-1 N1_R Sample source:Right atrial tissue | Treatment:Non-ischemia control Genotype=WT; RAW_FILE_NAME(Raw_file_name)=N1_R.mzML SUBJECT_SAMPLE_FACTORS Control-2 N2_R Sample source:Right atrial tissue | Treatment:Non-ischemia control Genotype=WT; RAW_FILE_NAME(Raw_file_name)=N2_R.mzML SUBJECT_SAMPLE_FACTORS Control-3 N3_R Sample source:Right atrial tissue | Treatment:Non-ischemia control Genotype=WT; RAW_FILE_NAME(Raw_file_name)=N3_R.mzML SUBJECT_SAMPLE_FACTORS Control-4 N4_R Sample source:Right atrial tissue | Treatment:Non-ischemia control Genotype=WT; RAW_FILE_NAME(Raw_file_name)=N4_R.mzML SUBJECT_SAMPLE_FACTORS IS-25-1 IS25_A Sample source:Right atrial tissue | Treatment:"Ischemia for 25 mins, followed by reperfusion for 30 mins" Genotype=WT; RAW_FILE_NAME(Raw_file_name)=IS25_A.mzML SUBJECT_SAMPLE_FACTORS IS-25-2 IS25_B Sample source:Right atrial tissue | Treatment:"Ischemia for 25 mins, followed by reperfusion for 30 mins" Genotype=WT; RAW_FILE_NAME(Raw_file_name)=IS25_B.mzML SUBJECT_SAMPLE_FACTORS IS-25-3 IS25_C Sample source:Right atrial tissue | Treatment:"Ischemia for 25 mins, followed by reperfusion for 30 mins" Genotype=WT; RAW_FILE_NAME(Raw_file_name)=IS25_C.mzML SUBJECT_SAMPLE_FACTORS IS-25ins-1 IS25_INS_A Sample source:Right atrial tissue | Treatment:Preperfusion of insluin for 30 mins before ischemia and reperfusion Genotype=WT; RAW_FILE_NAME(Raw_file_name)=IS25_INS_A.mzML SUBJECT_SAMPLE_FACTORS IS-25ins-2 IS25_INS_B Sample source:Right atrial tissue | Treatment:Preperfusion of insluin for 30 mins before ischemia and reperfusion Genotype=WT; RAW_FILE_NAME(Raw_file_name)=IS25_INS_B.mzML SUBJECT_SAMPLE_FACTORS IS-25ins-3 IS25_INS_C Sample source:Right atrial tissue | Treatment:Preperfusion of insluin for 30 mins before ischemia and reperfusion Genotype=WT; RAW_FILE_NAME(Raw_file_name)=IS25_INS_C.mzML #COLLECTION CO:COLLECTION_SUMMARY Rat hearts were isolated for ex vivo Langendorff perfusion. After the treatments CO:COLLECTION_SUMMARY were applied, right atrial tissue was collected and frozen at -80°C until IC-MS CO:COLLECTION_SUMMARY analysis CO:SAMPLE_TYPE Heart #TREATMENT TR:TREATMENT_SUMMARY Ex vivo hearts were pre-perfused with or without insulin, followed by ischemia TR:TREATMENT_SUMMARY induced by the cessation of perfusion. After 25 minutes of ischemia, hearts TR:TREATMENT_SUMMARY underwent 30 minutes of reperfusion. Right atrial tissue was then collected for TR:TREATMENT_SUMMARY IC-MS analysis #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Tissue were snap frozen in liquid nitrogen, then homogenized with Precellys SP:SAMPLEPREP_SUMMARY Tissue Homogenizer. Certain volumes of homogenate were used to make sure same SP:SAMPLEPREP_SUMMARY amount of tissue were extracted. Metabolites were extracted using ice-cold 80/20 SP:SAMPLEPREP_SUMMARY (v/v) methanol/water with 0.1% ammonium hydroxide. Extracts were vortexed SP:SAMPLEPREP_SUMMARY vigorously for 2 mins, centrifuged at 17,000 g for 10 min at 4°C, and SP:SAMPLEPREP_SUMMARY supernatants were transferred to clean tubes, followed by evaporation to dryness SP:SAMPLEPREP_SUMMARY under nitrogen. Dried extracts were reconstituted in 100 μL of 1mM potassium SP:SAMPLEPREP_SUMMARY hydroxide (KOH) in deionized water. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY ion chromatography with high resolution mass spectrometry CH:CHROMATOGRAPHY_TYPE Ion exchange CH:INSTRUMENT_NAME Thermo Dionex ICS-6000+ CH:COLUMN_NAME Thermo IonPac AS11 CH:SOLVENT_A Water CH:SOLVENT_B KOH CH:FLOW_GRADIENT 0-5 min, 1% MPB; 5-25 min, 1-35% MPB; 25-39 min, 35-99% MPB; 39-49 min, 99% MPB; CH:FLOW_GRADIENT 49-50 min, 99-1% MPB CH:FLOW_RATE 360uL/min CH:COLUMN_TEMPERATURE 35 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Ortibrap IQ-X MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS . Data were acquired using a Thermo Orbitrap IQ-X Tribrid Mass Spectrometer MS:MS_COMMENTS under ESI negative ionization. All the raw data files were imported into Thermo MS:MS_COMMENTS Trace Finder 5.1 software for final analysis #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Area under the Curve MS_METABOLITE_DATA_START Samples N1_R N2_R N3_R N4_R IS25_A IS25_B IS25_C IS25_INS_A IS25_INS_B IS25_INS_C Factors Sample source:Right atrial tissue | Treatment:Non-ischemia control Sample source:Right atrial tissue | Treatment:Non-ischemia control Sample source:Right atrial tissue | Treatment:Non-ischemia control Sample source:Right atrial tissue | Treatment:Non-ischemia control Sample source:Right atrial tissue | Treatment:"Ischemia for 25 mins, followed by reperfusion for 30 mins" Sample source:Right atrial tissue | Treatment:"Ischemia for 25 mins, followed by reperfusion for 30 mins" Sample source:Right atrial tissue | Treatment:"Ischemia for 25 mins, followed by reperfusion for 30 mins" Sample source:Right atrial tissue | Treatment:Preperfusion of insluin for 30 mins before ischemia and reperfusion Sample source:Right atrial tissue | Treatment:Preperfusion of insluin for 30 mins before ischemia and reperfusion Sample source:Right atrial tissue | Treatment:Preperfusion of insluin for 30 mins before ischemia and reperfusion 2-3-diphosphoglycerate 79455 42629 75541 64677 537141 298355 159982 95322 598 209548 2-phosphoglycerate 153009248 148281760 212914992 186163280 395294528 309777600 241341136 266677984 169141216 315282784 fructose-1-6-bisphosphate 29597790 33768644 38508476 23318364 73507856 39962824 46024072 229496752 84093128 246919616 fructose-1-phosphate 90678528 69517592 100102816 37279084 149745216 143083200 81122312 334595264 142397936 251293392 fructose-6-phosphate 53933704 26435534 44562264 22421714 5708310 5282496 6969934 43383964 44369244 31621640 glucose 172761008 140975056 170566880 121941816 131184712 133140496 102456528 88508536 72175128 75849912 glucose-1-6-bisphosphate 28568956 32665090 37328688 22774590 70247392 38840220 45539892 225772816 83618968 241199696 glucose-1-phosphate 95901128 46752548 93138912 64033160 100296624 68979232 66811004 189363728 63886468 138221088 glucose-6-phosphate 296201536 152135552 273608096 145590256 25724792 37083808 42275552 245992416 269973824 199567264 glycerol-3-phosphate 85650864 132052456 134144088 157073168 267290432 270934112 246990464 297468128 340421120 358712064 lactate 845165824 741953088 886921984 675995776 832533824 938346112 798849664 789052288 709031488 685560000 mannose-6-phosphate 49973324 28917562 47987888 28747214 13547377 13902931 10343933 31299056 54806604 39744216 phosphoenolpyruvate 7760490 10504255 8027153 13780738 28307904 35107924 22607236 18713498 16244946 25130388 glyoxylate 1785975 2023617 2395255 2344415 3481939 2540352 1855932 1811263 935399 1175432 pyruvate 7001874 8005141 9863521 5919133 10142625 8773454 5897293 13873098 1292531 2163205 2-hydroxyglutarate-(2-HG) 19826654 19772052 28266724 16133978 27694012 29287200 17359056 17255728 4779944 6875315 2-oxoglutarate-(a-KG) 9430006 10029751 14523389 9112014 18686150 17876808 11191249 10920238 4024992 6267301 aspartate-(Asp) 1543245056 2056456576 1870213248 1669527296 1832026368 1394032000 1271651840 1072042752 508361568 552801088 cis-aconitate 1160317 1288833 1797354 1338520 1059435 1613843 955227 890645 675130 403562 citrate 674350528 710330048 918721600 602988928 710725824 810033216 610038720 604810816 287887456 299315616 fumarate 22006 53274 100771 95000 4327361 4277231 2606573 5026683 8751714 10118833 glutamate-(Glu) 2197594624 2358827776 2052887808 1910655360 2690671104 2550794240 2260112896 1800995712 1017630272 1369263872 isocitrate 15271238 18459058 20544950 12886180 17509358 20667700 15240601 16242815 8894958 9479507 malate 386819456 430996320 523150048 498001216 770757120 561225984 396623648 431671904 225212528 271847488 oxaloacetate-(OAA) 157404 165970 180756 131226 139722 158065 126370 131094 60759 58472 succinate 186699488 147192624 245191664 192469840 216194992 260146176 218149744 227809680 163583248 149371856 succinate-semialdehyde 14074806 14424119 15738134 6832784 16622810 13866509 9780805 10460361 11558500 10822054 deoxyribose-5-phosphate 220249 183861 171240 102374 45027 33025 24385 34159 27354 29939 erythrose-4-phosphate 10947551 6740107 10423941 6782671 2044231 2790248 2465479 10185033 10434335 7864483 gluconate 33536524 25869642 32583814 18086294 53411852 23918956 18319812 19921336 16924286 35355944 gluconate-6-phosphate 381251 406163 1030794 1092876 204640 239365 384579 1410652 3570228 3511421 glucono-1-5-lactone 929397 820040 2170065 855468 1335383 1400375 1119851 809304 326065 577388 ribose-1-5-bisphosphate 955571 973340 774231 3022341 1243340 1208797 1115282 1036253 384628 512905 ribose-1-phosphate 852924992 800520960 712123712 1115785856 824314752 656794112 544494144 640913408 457826368 548060224 sedoheptulose-1-7-bisphosphate 9953333 10196171 11827496 17242882 30962288 23936820 23235394 28381626 6645823 15501013 sedoheptulose-7-phosphate 139633568 121484896 153953520 173832960 271565216 249250560 241748704 233269280 41155708 98238984 adenylsuccinate 6788475 5641843 10289769 26284554 33592008 17747740 10872679 13099031 4016848 3251700 adenosine-5-diphosphate-(ADP) 600954112 578139584 911087168 914901568 1321916416 1097078528 819795456 1199493376 287239040 349383392 ADP-glucose 104196 68706 159438 224783 634454 595713 1101715 742127 72725 105069 ADP-ribose 1195035 693963 4281682 2380319 13383824 21133746 14177585 20244386 992057 2796897 adenosine-5-monophosphate-(AMP) 153674432 161840528 336286912 397033248 749946112 813883904 636837888 1201815168 155637120 353548672 adenosine-5-triphosphate-(ATP) 15159254 12001194 25939454 35618004 33154724 54028628 30815056 70672360 813489 11230735 cytidine-5-diphosphate-(CDP) 56270 54483 163647 344021 808122 1115739 354280 2031430 57537 117518 CDP-ethanolamine 44096 26818 50806 49286 147496 406855 207604 823722 33835 37408 cytidine-5-monophosphate-(CMP) 551155 513992 839887 1308281 2378789 3401036 2126039 7471477 543500 1370661 CMP-N-acetylneuraminate 14196930 14916186 15860111 10228831 19420086 16002959 12760322 10049517 3865144 5660017 cytidine-5-triphosphate-(CTP) 4866 15831 17508 52420 80900 164560 56953 131447 787 10424 cyclic-AMP-(cAMP) 320271 271984 632057 367915 1251242 1196061 1047881 688860 169881 258826 2-deoxyguanosine-5-diphosphate-(dGDP) 600954112 578139584 911087168 914901568 1321916416 1097078528 819795456 1199493376 287239040 349383392 2-deoxyguanosine-5-monophosphate-(dGMP) 153674432 161840528 336286912 397033248 749946112 813883904 636837888 1201815168 155637120 353548672 2-deoxyguanosine-5-triphosphate-(dGTP) 15159254 12001194 25939454 35618004 33154724 54028628 30815056 70672360 813489 11230735 2-deoxythymidine-5-monophosphate-(dTMP) 214531 139197 281095 195223 351697 263868 121187 167241 28016 35287 flavin-adenine-dinucleotide-(FAD) 206825 162677 298573 191347 488651 451209 250619 248048 86224 113392 flavin-mononucleotide-(FMN) 10367906 10045625 14198440 8359556 13766463 11789622 7553245 7172382 4200299 5810315 guanosine-5-diphosphate-(GDP) 16293034 15040723 23996796 27118010 28053764 36729960 35371332 38941180 2788000 15653361 GDP-mannose 147899 110699 263635 331636 581824 1906706 1444460 2222486 69810 153466 guanosine-5-monophosphate-(GMP) 81886960 62308952 86209792 110355656 173480480 122134008 160206144 183444752 63381552 113089584 guanosine-5-triphosphate-(GTP) 128066 89454 175067 344565 222918 771584 514379 995639 2368 135326 inosine-5-monophosphate-(IMP) 31863884 26022714 46746656 60978476 262171104 232033600 243125456 241183968 55097352 114763952 adenosine-3-phosphate-5-phosphosulfate 133444 54673 216074 287229 228505 526952 311748 755343 1579 113999 pyridoxal-5-phosphate-(P5P) 1988036 2229519 2791561 4270632 5740169 5117598 3856203 3581713 843805 1777350 thymidine-5-monophosphate-(TMP) 214531 139197 281095 195223 351697 263868 121187 167241 28016 35287 uridine-5-diphosphate-(UDP) 787648 864093 1475705 2420762 4661333 4756014 2902822 8455239 559268 2035923 UDP-N-acetylgalactosamine-(UDP-GalNAc) 148812 123093 148117 157547 405095 963392 637373 955632 48812 90341 UDP-N-acetylglucosamine-(UDP-GlcNAc) 503013 465408 644033 657184 2907471 6807864 3925137 8418296 283694 566451 uridine-5-monophosphate-(UMP) 8216061 8945502 11425861 13506101 47518636 52400932 46084212 123716480 21100292 56944564 urate 569268672 526352736 565666048 499025248 680900352 535101088 409407424 334159776 134912592 213480880 uridine 31231960 28383626 24092934 25283468 21059958 17018314 17446326 19230338 14522982 15065908 uridine-5-triphosphate-(UTP) 21250 27289 23224 55901 85895 119587 54865 144630 6183 24401 xansthosine-5-monophosphate-(XMP) 153289 158988 362457 766129 420543 577410 578905 344983 171190 259316 riboflavin-cyclic-4-5-phosphate 31269516 26104756 46009696 28405882 66183244 63484092 38101568 41846860 17463114 21584304 2-aminomuconate 1591688 1232957 1731206 476682 2232858 1802263 1117338 1050992 671939 577382 2-methylglutarate 11059337 10593745 8641829 14490217 9144638 15780589 9243160 2085133 308264 360579 2-propylglutarate 863393 409391 401347 195270 401084 914376 361925 351520 225885 394415 3-hydroxymethylglutarate 12972279 11186756 13443622 8312697 12216090 11477976 8230570 8820062 8976901 8433697 4-hydroxyglutamate-semialdehyde 2291843072 2451983616 2136098560 1967522816 2824298496 2681925120 2369944320 1893353088 1209017856 1577142272 gamma-glutamylalanine-(g-Glu-Ala) 4773608 8185880 8308189 16407043 9005474 14267919 5385754 7045702 3505518 3041858 gamma-glutamyltaurine 352877888 331720928 343446112 384259808 477849856 466130528 373982976 154653376 61976816 120157096 acetylcarnitine 2201623 2730944 3356883 2484499 4661602 4543551 2213876 1511288 904527 974873 aspartylglutamate-(Asp-Glu) 4592117 5418941 5402519 4330213 4660162 4213819 3037791 2463439 1097465 1254895 citryl-glutamate 11191936 14072757 16701301 16344486 14904690 7034648 12629420 20348086 3646947 5239406 cysteate 1949783 1541449 1528833 590756 2433729 1853083 1252974 1085967 486871 486523 glutamylglutamate-(Glu-Glu) 60274240 74222992 53421428 58295928 61184248 66246696 59468580 32320722 12374281 22841324 glutathione--reduced-(GSH) 840814528 910713856 1180805120 1237179520 1530605568 1401034880 1190720000 1359364992 1138835584 1040480768 homocysteinesulfinate 39949088 32725162 29657168 86640496 33039450 26328718 38060408 10895069 3271785 5750132 ketoleucine 3650089 4824063 4442211 4694928 3780065 4373274 4344654 4100761 4483152 4077184 N-acetylaspartate 64537824 50633232 71673320 21768772 90687216 71945664 47347220 44873520 29857660 26646954 N-acetylglutamate 564924 528532 371119 292523 375832 300386 298603 250168 167474 206733 N-acetylmethionine 816164 675917 606192 231939 954572 606554 435566 365161 663552 531378 N-acetylneuraminate 40933340 39739652 45921116 31078090 34269352 40043524 23192196 29621496 26757242 15803601 N-formylmethionine 596913 645559 821143 514161 1193482 842172 657094 537616 209697 182729 orotate 1021938 741955 1603364 1517332 3002154 2651525 1484260 2326739 565001 685407 orotidine 1358818 1013068 1239020 417227 1457447 823966 656047 692513 281032 325700 pantothenate 9113673 12931137 9224794 11934379 16114386 14343420 10394678 11465930 12754976 11324033 phosphocreatine 1241650 954223 1236357 4669336 16253935 34748988 27247106 5809538 4899199 10415765 pyroglutamate-(pGlu) 62332576 81994864 60761620 61596836 37898732 53132836 35078040 31199384 19548008 20420486 taurine 1378469760 1395671424 1478840448 1618711040 1723109888 1826112640 1720672000 1559327360 1030693056 1219982976 ureidosuccinate 12957 13117 8541 10904 122014 156226 110430 490630 290619 267910 2-hydroxybutyrate 54183 67932 57290 40464 7039 102270 59539 19997 15708 42899 3-hydroxybutyrate 997820 875091 1757209 1980911 2740045 3998956 2715107 2056779 1923105 1694454 butyrate 402776 633016 625437 376484 410283 528339 344385 349204 133409 249555 glycerophosphocholine 229200 882375 486060 1393354 699555 729365 375718 341724 126309 162863 O-phosphoethanolamine 1559314 1082555 1058820 2385206 7586706 5983645 5338607 6642982 7880747 9861500 1-pyrroline-2-carboxylate 996792 1747827 2496915 1555372 1489642 2188172 1472719 2188334 904202 741313 2-hydroxyadipate 12972279 11186756 13443622 8312697 12216090 11477976 8230570 8820062 8976901 8433697 5-phosphoribosyl-N-formylglycinamide 52858 33287 9991 43433 3865369 417525 199531 434557 167895 252421 galactarate 414550 414207 412679 332502 566300 472975 317822 337553 107057 193413 galactose-1-phosphate 264698512 137625520 283705856 251661776 348044512 342587008 230916112 207153472 58134836 70222344 galacturonate 16055038 11936322 19726434 5187457 7760151 8820942 5344910 5572576 1712063 1821827 glucarate 9935665 11406922 12335322 16372057 34007164 34421312 27380572 25647716 12784310 20235952 glucuronate-1-phosphate 13234708 7870455 9245221 15560802 13776288 13328439 12995512 12473288 11147911 10965511 glutarate 44260 59154 122196 54693 191489 157645 75724 128597 70717 47450 glycerate 72134672 75105360 100829272 49503404 45250644 51064332 38255196 39902120 10958206 16857726 hydroxypropanedioate 29207478 39485500 31663658 54471612 58038260 45330232 36405900 32712324 9893277 21384060 maleate 182200 105275 267360 334713 5444277 5891801 2422520 4503254 9396357 10368793 malonate 414883 544025 539651 406054 616258 666197 424590 601298 318971 372364 oxalate 14429846 16590293 16734775 21916796 35241300 31516060 25380998 22965006 13678130 18569678 ribonate 21481090 24552264 26749444 36758364 76027536 78621040 63643084 58531276 28369890 44925288 threonate 92261360 119259120 95419872 126568480 73879432 63112228 58068352 47633916 26459742 37025196 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name 2-3-diphosphoglycerate 2-phosphoglycerate fructose-1-6-bisphosphate fructose-1-phosphate fructose-6-phosphate glucose glucose-1-6-bisphosphate glucose-1-phosphate glucose-6-phosphate glycerol-3-phosphate lactate mannose-6-phosphate phosphoenolpyruvate glyoxylate pyruvate 2-hydroxyglutarate-(2-HG) 2-oxoglutarate-(a-KG) aspartate-(Asp) cis-aconitate citrate fumarate glutamate-(Glu) isocitrate malate oxaloacetate-(OAA) succinate succinate-semialdehyde deoxyribose-5-phosphate erythrose-4-phosphate gluconate gluconate-6-phosphate glucono-1-5-lactone ribose-1-5-bisphosphate ribose-1-phosphate sedoheptulose-1-7-bisphosphate sedoheptulose-7-phosphate adenylsuccinate adenosine-5-diphosphate-(ADP) ADP-glucose ADP-ribose adenosine-5-monophosphate-(AMP) adenosine-5-triphosphate-(ATP) cytidine-5-diphosphate-(CDP) CDP-ethanolamine cytidine-5-monophosphate-(CMP) CMP-N-acetylneuraminate cytidine-5-triphosphate-(CTP) cyclic-AMP-(cAMP) 2-deoxyguanosine-5-diphosphate-(dGDP) 2-deoxyguanosine-5-monophosphate-(dGMP) 2-deoxyguanosine-5-triphosphate-(dGTP) 2-deoxythymidine-5-monophosphate-(dTMP) flavin-adenine-dinucleotide-(FAD) flavin-mononucleotide-(FMN) guanosine-5-diphosphate-(GDP) GDP-mannose guanosine-5-monophosphate-(GMP) guanosine-5-triphosphate-(GTP) inosine-5-monophosphate-(IMP) adenosine-3-phosphate-5-phosphosulfate pyridoxal-5-phosphate-(P5P) thymidine-5-monophosphate-(TMP) uridine-5-diphosphate-(UDP) UDP-N-acetylgalactosamine-(UDP-GalNAc) UDP-N-acetylglucosamine-(UDP-GlcNAc) uridine-5-monophosphate-(UMP) urate uridine uridine-5-triphosphate-(UTP) xansthosine-5-monophosphate-(XMP) riboflavin-cyclic-4-5-phosphate 2-aminomuconate 2-methylglutarate 2-propylglutarate 3-hydroxymethylglutarate 4-hydroxyglutamate-semialdehyde gamma-glutamylalanine-(g-Glu-Ala) gamma-glutamyltaurine acetylcarnitine aspartylglutamate-(Asp-Glu) citryl-glutamate cysteate glutamylglutamate-(Glu-Glu) glutathione--reduced-(GSH) homocysteinesulfinate ketoleucine N-acetylaspartate N-acetylglutamate N-acetylmethionine N-acetylneuraminate N-formylmethionine orotate orotidine pantothenate phosphocreatine pyroglutamate-(pGlu) taurine ureidosuccinate 2-hydroxybutyrate 3-hydroxybutyrate butyrate glycerophosphocholine O-phosphoethanolamine 1-pyrroline-2-carboxylate 2-hydroxyadipate 5-phosphoribosyl-N-formylglycinamide galactarate galactose-1-phosphate galacturonate glucarate glucuronate-1-phosphate glutarate glycerate hydroxypropanedioate maleate malonate oxalate ribonate threonate METABOLITES_END #END