#METABOLOMICS WORKBENCH yxw_524521_20240927_090138 DATATRACK_ID:5239 STUDY_ID:ST003537 ANALYSIS_ID:AN005809 PROJECT_ID:PR002177 VERSION 1 CREATED_ON October 17, 2024, 10:59 pm #PROJECT PR:PROJECT_TITLE Metabolomic analysis of fluorescent hairy roots overexpressing the Gretchen PR:PROJECT_TITLE Hagen 3 genes enhancing soybean resistance to cyst nematodes PR:PROJECT_SUMMARY Soybean root samples were collected, target metabolites were extracted, standard PR:PROJECT_SUMMARY curves were established, methodology verification was performed, LC-MS/MS PR:PROJECT_SUMMARY metabolites were detected, and data were analyzed.Metabolome analysis was PR:PROJECT_SUMMARY conducted on FHRs overexpressing these genes to investigate how plant hormones PR:PROJECT_SUMMARY play a role in soybean resistance to SCN. These results provide a new framework PR:PROJECT_SUMMARY for understanding the interaction between soybean and SCN. PR:INSTITUTE Shenyang Agricultural University PR:LAST_NAME Yang PR:FIRST_NAME Xiaowen PR:ADDRESS College of Plant Protection PR:EMAIL 2020200130@stu.syau.edu.cn PR:PHONE 15702428682 #STUDY ST:STUDY_TITLE Metabolomic analysis of fluorescent hairy roots overexpressing the Gretchen ST:STUDY_TITLE Hagen 3_3 genes enhancing soybean resistance to cyst nematodes ST:STUDY_SUMMARY The Gretchen Hagen 3 genes maintain endogenous hormone homeostasis by ST:STUDY_SUMMARY conjugating excess hormones with amino acids. Herein, we identified the members ST:STUDY_SUMMARY of the GH3 family in soybeans and analyzed their phylogeny, gene duplication, ST:STUDY_SUMMARY structure, domains, conserved motifs, cis-elements in promoter regions for ST:STUDY_SUMMARY stress responses, and functional characteristics. We found that GH3 genes are ST:STUDY_SUMMARY induced by pathogens in Group-II. Furthermore, 8 out of 16 Group-II genes ST:STUDY_SUMMARY responded to cyst nematode infection. Through functional analysis of eight GmGH3 ST:STUDY_SUMMARY genes via overexpression, they play a negative role in soybean resistance to ST:STUDY_SUMMARY cyst nematode. In addition, our metabolomic analysis showed that overexpression ST:STUDY_SUMMARY of Glyma.02G125600, Glyma.17G165500, and Glyma.13G284600 affected the content of ST:STUDY_SUMMARY salicylic acid and jasmonic acid. Our findings clarify the functional features ST:STUDY_SUMMARY of GH3 genes and reveal their involvement in plant hormone signaling pathways. ST:STUDY_SUMMARY This provides valuable insights into the complex molecular mechanisms underlying ST:STUDY_SUMMARY the interaction between soybeans and cyst nematode. ST:INSTITUTE Shenyang Agricultural University ST:LAST_NAME yang ST:FIRST_NAME wen ST:ADDRESS College of Plant Protection ST:EMAIL 2020200130@stu.syau.edu.cn ST:PHONE 15702428682 #SUBJECT SU:SUBJECT_TYPE Plant SU:SUBJECT_SPECIES Glycine max SU:TAXONOMY_ID 3847 #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - CK-1 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-2 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-3 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-4 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-5 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-6 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_3-1 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_3-2 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_3-3 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_3-4 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_3-5 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_3-6 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- #COLLECTION CO:COLLECTION_SUMMARY Liquid nitrogen grinding samples, weighing a certain amount of samples into mass CO:COLLECTION_SUMMARY spectrometry water, vortex mixing, as diluted samples; Take a dilution sample of CO:COLLECTION_SUMMARY 100μL and add 400 μL precipitator (acetonitrile: Water =1:1), vortex mixing, CO:COLLECTION_SUMMARY extraction at 4°C for 30 min, centrifugation at 12000 rpm and 4°C for 10 CO:COLLECTION_SUMMARY min, taking 300 μL supernatant slowly through the extraction column, adding 500 CO:COLLECTION_SUMMARY μL eluent (30% acetonitrile), slowly through the extraction column, mixing the CO:COLLECTION_SUMMARY two times of flow solution, LC-MS analysis. CO:SAMPLE_TYPE Plant roots #TREATMENT TR:TREATMENT_SUMMARY Take a dilution sample of 100 μL and add 400 μL precipitator (acetonitrile: TR:TREATMENT_SUMMARY Water = 1:1), vortex mixing, extraction at 4°C for 30 min, centrifugation at TR:TREATMENT_SUMMARY 12000 rpm and 4°C for 10 min, taking 300 μL supernatant slowly through the TR:TREATMENT_SUMMARY extraction column, adding 500 μL eluent (30% acetonitrile), slowly through the TR:TREATMENT_SUMMARY extraction column, mixing the two times of flow solution, LC-MS analysis. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Take a dilution sample of 100 μL and add 400 μL precipitator (acetonitrile: SP:SAMPLEPREP_SUMMARY Water =1:1), vortex mixing, extraction at 4°C for 30 min, centrifugation at SP:SAMPLEPREP_SUMMARY 12000 rpm and 4°C for 10 min, taking 300 μL supernatant slowly through the SP:SAMPLEPREP_SUMMARY extraction column, adding 500 μL eluent (30% acetonitrile), slowly through the SP:SAMPLEPREP_SUMMARY extraction column, mixing the two times of flow solution, LC-MS analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME AB Sciex QTRAP 6500+ CH:COLUMN_NAME Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 μm) CH:SOLVENT_A 100% Water; 5mM Ammonium Acetate (Adjusted to pH 4.5 with Acetic Acid) CH:SOLVENT_B 100% Acetonitrile CH:FLOW_GRADIENT The initial proportion of mobile phase B is set at 15%, which then linearly CH:FLOW_GRADIENT increases at a rate of 0.8% per minute until it reaches 45%, where it is held CH:FLOW_GRADIENT for 5 minutes to adequately elute moderately polar metabolites. Afterward, it CH:FLOW_GRADIENT increases at a rate of 2% per minute up to 90%, and finally remains at this CH:FLOW_GRADIENT composition for an additional 5 minutes to clean the chromatographic column and CH:FLOW_GRADIENT collect less polar metabolites. CH:FLOW_RATE 0.35 mL/min CH:COLUMN_TEMPERATURE 35°C #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME AB Sciex QTRAP 6500+ MS:INSTRUMENT_TYPE QTRAP MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS The concentration series of the standard solution were detected by LC-MS, and MS:MS_COMMENTS the ratio of the concentration of the standard product to the internal standard MS:MS_COMMENTS was taken as the horizontal coordinate and the ratio of the peak area of the MS:MS_COMMENTS standard product to the internal standard was taken as the vertical coordinate MS:MS_COMMENTS to investigate the linearity of the standard solution. QC samples were tested by MS:MS_COMMENTS inserting a number of needles at intervals in each batch of samples. The RSD of MS:MS_COMMENTS all QC samples was calculated with the concentration of the standard substance MS:MS_COMMENTS to evaluate stability. According to the established sample pretreatment and MS:MS_COMMENTS instrumental analysis methods, all samples were quantitatively analyzed. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak area MS_METABOLITE_DATA_START Samples CK-1 CK-2 CK-3 CK-4 CK-5 CK-6 GH3_3-1 GH3_3-2 GH3_3-3 GH3_3-4 GH3_3-5 GH3_3-6 Factors Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Cytidine 12980000 16550000 10360000 11630000 19200000 17860000 49760000 24380000 30950000 26920000 21970000 13580000 Azelaic acid 19140 40800 30080 27920 40720 40920 18030 12340 25590 16580 20660 17260 trans-Zeatin riboside 110300 141200 81510 69920 116800 110100 108700 163900 151800 134300 143900 128700 Methyl linoleate 57470 63090 36931 47950 53760 45143 56770 65120 65850 82320 61170 73890 SDMA 264900 511000 297400 189600 231100 241500 373300 389900 440900 306800 485500 363800 5-Methylcytidine 174800 269200 195000 160900 200300 231900 542200 320400 354400 430800 354400 211700 Calycosin-7-O-beta-D-glucoside 375300 377100 611600 569400 446600 1621000 123000 119705 266900 131617 210000 103800 Schaftoside 16639 27320 19480 20902 25050 40270 9497 9626 27490 10120 14388 18480 Corylin 169700 575700 134100 184300 520600 588300 74580 48820 125600 24100 112700 198600 Uracil 3353000 3483000 2478000 2670000 3454000 2862000 3458000 4093000 5069000 3640000 4010000 3575000 DL-Citrulline 1907000 2725000 1184000 1200000 1630000 1014000 2721000 4121000 3399000 2402000 3115000 1744000 L-arginine 845300 2270000 1021000 1069000 1310000 1117000 2907000 2555000 1328000 1880000 2432000 1782000 Glycyl-L-leucine 443600 727400 358900 354000 482500 461300 516700 711700 686400 571400 591900 678900 delta-Hexanolactone 1818000 2983000 2551000 3200000 2528000 4592000 1078000 1132000 1317000 1302000 1510000 1985000 Sattabacin 42380 40250 49080 82870 56250 77640 18580 15220 17210 29280 23700 26280 Nicotinate ribonucleoside 607300 570400 891900 902200 1053000 1081000 482400 385500 694200 569900 705700 744700 2'-O-methyladenosine 157900 352200 164700 215600 394900 353500 408600 414800 412300 406100 542900 367600 N-Acetyl-L-leucine 23500000 17960000 18650000 22140000 19670000 19720000 18980000 17600000 21350000 14290000 14320000 13430000 N-Acetyl-L-histidine 43621 49420 38300 68220 70850 42410 27420 26540 44818 44717 36780 38100 iP9G 274300 535600 448300 617600 536200 527600 166300 273600 482100 251900 264800 429100 Indole-3-acetamide 18920 40640 33500 47330 40000 24000 63780 83440 62740 62410 63020 47780 1-Monopalmitin 70280 63460 63250 47680 46750 44260 34960 46600 36440 37790 29960 41050 Eriodictyol-7-O-glucoside 4107 2819 5989 5352 7710 4290 3903 1943 3413 3612 3131 2780 dUDP 130400 188900 180700 203500 116000 168000 48410 46910 56420 61710 93270 82840 10-Formyl-THF 755300 672800 712700 1084000 1199000 1119000 562600 694000 668100 572600 696800 868700 Tetraethylammonium fluoride 5446000 6756000 4607000 3076000 6323000 6163000 10210000 8755000 13010000 10690000 10200000 6468000 1-Methylhistidine 449500 302100 123100 121700 244700 198200 390800 1154000 404400 857600 288900 336100 S-Adenosyl-methionine 581800 704700 343300 225100 181800 316700 767500 1003000 786200 732600 602800 535100 Pro-Leu 228200 238500 129900 365700 295500 300400 156292 159200 170700 116700 142300 208100 N2-Acetyl-L-lysine 106900000 131400000 116600000 122500000 92280000 149700000 59380000 64240000 65470000 62760000 71920000 87380000 L-Alanyl-L-leucine 689500 1228000 595800 637100 884600 838700 911100 1245000 1183000 988800 979200 1162000 Gly-Tyr 74110 106700 64110 56170 79640 65930 94980 125400 118100 116500 105100 133600 Gly-Phe 173900 320400 169400 172100 266600 230900 293700 347900 333800 288400 309800 328300 Dl-Norleucine 28700000 38470000 26450000 29280000 31100000 37870000 19780000 27810000 33970000 18550000 23560000 26950000 2-(Dimethylamino)Guanosine 50190 160200 47010 46410 142200 121600 154700 121700 170100 202900 203500 121500 methyl oxo pentanoate 80050 129800 112400 97320 148900 137100 89890 71240 73540 73540 71800 55890 Trimethylamine N-oxide 19710 43350 21510 17140 25910 30080 51640 27380 28540 62610 53470 29230 D-Myo-inositol 4-monophosohate 5644000 4072000 4238000 5203000 2490000 4772000 7499000 6890000 7182000 5817000 4327000 5473000 2-Deoxy-D-galactose 4274000 5436000 4677000 5089000 3807000 6174000 2464000 2539000 3036000 2752000 3288000 3663000 dAMP 97590 65810 67830 125200 158300 135900 47500 57200 104900 54600 46350 36830 1-Methylguanosine 36830 36410 16560 13530 28920 23900 61350 32840 47940 45399 38840 33810 Scopine 22750 22690 28640 31340 22590 32720 18170 14954 22530 13310 20620 21280 Stachydrine 2131000 1408000 836100 867000 4667000 2275000 215700 169900 817800 671000 737400 1075000 Cephalotaxine 11133 12070 14080 8054 11980 34480 4253 6262 7819 5787 3831 7645 7-Ethylcamptothecin 43419 108154 184800 365300 697000 783200 19969 41205 37342 12833 29542 55160 Berberine 3968 8045 4029 19850 7987 31150 2956 3010 3648 4766 5355 4648 Aristolone 5827 6341 15040 13760 3612 10530 4979 5955 4437 3223 2795 5732 Homoplantaginin 182200 127700 191400 239000 312300 548100 103500 22360 84880 105300 112000 74000 Tabersonine 242600 333800 203200 474700 601400 974100 111100 33150 177400 154800 236100 48770 Aloperine 7873 7236 9446 9516 9533 7409 7768 12800 13590 17790 10248 11242 3-Hydroxy-9,10-Dimethoxypterocarpan 26070 71720 28320 43180 88870 95260 34770 6173 46420 6787 31360 19580 Indirubin 9606 26930 3357 4406 5280 13540 17390 67850 24940 25790 22430 11870 Homoharringtonine 22470 44770 22090 26440 36950 32740 7530 34146 18450 9546 16550 11638 Pratensein-7-O-glucoside 89760 132100 236100 137700 294300 351100 44710 57560 77110 97670 96620 84320 Prim-O-glucosylcimifugin 113500 94080 344700 255900 290400 440200 54050 54540 40190 83392 149400 29240 Vincosamide 5630 2958 3510 3538 5611 5576 8610 13730 10940 8875 6527 5345 Oxysophocarpine 9034 13720 4927 3295 7558 16200 13180 51020 10350 23830 27800 9056 ladanein 10970 86840 12090 39590 82270 47160 8395 13407 9050 1438 13300 2293 5-O-p-Coumaroyl shikimic acid 14380 10120 19580 30880 19970 17460 4810 13340 7950 5346 10470 19520 Phellodensin F 19070 12810 16110 13150 40900 73570 17840 13546 4800 9874 3529 14580 Cocamidopropyl betaine 215600 233700 177200 176300 457000 474400 130200 152200 227900 139400 144700 82160 MAG (18 1) 5289 5666 3096 2133 2676 7611 7976 8680 3342 19410 6800 13310 Asp-phe 140700 205700 119200 109100 170400 164700 177100 234500 286900 211000 211100 231300 Diethyl glutarate 104000000 127300000 116600000 124600000 92760000 149700000 49700000 62010000 61470000 64850000 70960000 87520000 DL-threitol 145900 176800 117900 97500 161500 158900 106500 79320 106500 110500 97840 105400 Methionine sulfoxide 1179000 682400 841000 563300 366400 324400 951500 1247000 1945000 2037000 832800 567900 Sissotrin 810800 819200 1484000 1131000 1575000 3494000 290300 117300 460500 184800 829000 159200 Peonidin O-hexoside 93500 204000 256800 326600 358700 432000 48500 52930 59340 52860 121200 24640 Cyanidin 3-O-malonylhexoside 296000 123400 300500 344200 358500 725500 26310 37350 55670 84770 73470 45730 Pelargonidin 3-O-malonylhexoside 2363000 1302000 1489000 1256000 3303000 3796000 1016000 736800 925500 933900 935500 1406000 Delphinidin 3-O-Rutinoside 6016 9929 7571 12480 9894 14718 7646 3698 3486 4719 10580 5732 (-)-Norepinephrine N-hexoside 827200 929100 1426000 1484000 1359000 1880000 422500 620900 734300 666300 518300 546800 18-Nor-4,15-dihydroxyabieta-8,11,13-trien-7-one 12960 31300 30990 47620 75030 22360 12480 10350 15630 9567 19280 13170 1-Methyladenine 82090 145000 79080 76980 77270 56430 150800 165600 104200 116900 151800 104700 2-Methyladenosine 1712000 3067000 1851000 1555000 2413000 2562000 4290000 3791000 3353000 4249000 4668000 2658000 Apigenin O-malonylhexoside 16060000 15010000 14280000 10500000 31700000 32160000 9325000 10390000 11440000 10750000 8835000 11690000 Apo-13-zeaxanthinone 392400 303000 535500 564100 665500 523300 241200 292800 357400 337400 281600 482500 Chrysoeriol 5-O-hexoside 171200 127700 240700 213800 349400 668200 99940 65200 68340 173400 119000 72020 D-Ala-D-Ala 27040 41270 23220 29180 37010 27710 40830 40050 44310 57460 45460 40630 D-Sorbitol 377400 450500 306400 391900 344700 606500 233800 209100 361800 262300 305100 333800 Glu-Glu 34750 49820 41540 46610 44800 44280 14710 19600 51750 26400 18590 33880 GDP-L-fucose 14390 11380 10510 26050 8458 15100 3874 1652 6836 1537 7176 13310 IAA-Asp 455000 763100 789800 747100 1168000 1001000 408900 347500 726600 365500 429400 718900 IAA-Glu 58140 112300 77300 139500 98600 172600 57000 34090 70850 43990 67250 68620 Kaempferol 3-O-glucoside-2‘-O-rhamnoside 15630 10880 17010 32970 28720 35970 9895 4572 16000 11150 8649 3729 Luteolin O-malonylhexoside 1561000 982700 1744000 1734000 1707000 3994000 227600 204200 317100 621100 302900 255100 methylApigenin C-pentoside 152600 175000 148200 165700 114300 229800 96840 75370 80580 95590 111500 110000 methylQuercetin O-hexoside 639300 477700 329100 536900 636900 433300 263100 511300 294600 449700 284600 383000 N8-Acetylspermidine 36770 45990 35290 36530 36450 35200 30720 24250 24320 35220 26550 17070 N-Acetyl-5-hydroxytryptamine 19130 10840 7445 6093 7473 8185 18610 20820 49070 14720 9565 16730 Naringenin O-malonylhexoside 411600 401600 357100 272200 754600 863300 230500 244700 257500 268800 205800 305600 N-Sinapoylagmatine 49840 36740 35940 13310 25200 54980 61340 54950 44260 59660 42720 65820 N alpha-Acetyl-L-Arginine 43220 70310 49620 42950 46770 30430 19380 42550 37740 27720 26820 36400 Quercetin-3,4'-O-di-beta-glucopyranoside 41500 39920 57100 91460 69840 80100 21220 22230 28010 22590 21430 19300 Quercetin 5-O-hexoside 29040 60460 35480 51510 53250 69480 33500 12830 33950 45190 11590 36420 Selgin 5-O-hexoside 299400 186500 133800 332300 334900 233100 136400 180500 185700 196100 85870 170800 S-Sulfo-L-cysteine 21620 31730 13520 19090 15010 22320 25710 33470 37380 25820 38320 27270 Tricin O-malonylhexoside 44310 33650 26930 40110 78120 87490 18200 18820 20730 30390 35810 27880 Yamogenin 32150 34450 31880 32840 26760 45590 17250 15980 16090 34790 29760 30700 4-Hydroxybenzoate 111300 95930 143300 108400 193000 154500 65730 117400 104000 74640 52580 120500 Fustin 1857000 1480000 1642000 981300 1871000 2414000 1190000 872400 1511000 1311000 790300 1320000 N-Sinapoyl agmatine 11430 6428 12340 2087 9157 10780 17500 15020 15320 13300 17320 22030 Chrysoeriol O-malonylhexoside 275500 192500 405400 521900 648700 1206000 127600 94220 111900 150700 178600 123300 beta-Alanine 94890 127800 97500 76750 161200 146200 189700 215300 176900 169400 243000 120700 Histamine 3559000 6257000 4055000 4788000 4598000 6486000 11540000 6182000 8536000 6083000 7984000 5069000 Auramine 123500 119100 91090 149400 106700 109500 89050 63770 103800 74880 104700 101600 Benzyl cinnamate 15300 19980 24950 12210 15880 18910 25020 30230 53760 63360 27150 17660 Maltol 137000 153900 89510 103300 127600 131400 159800 165000 129900 153800 155400 298300 sn-Glycero-3-phosphocholine 241400 367900 198800 365500 422500 267000 161700 111400 273500 120600 132400 116400 Pyridoxine O-glucoside 259700 302800 367800 513600 400200 801900 143600 219500 253000 176300 154500 209100 methylLuteolin C-hexoside 8610 12320 6248 10400 4329 14930 3447 6297 5060 5751 4506 6721 Chrysoeriol 7-O-hexoside 149400 154900 210900 240800 376300 634200 142200 78050 91290 121200 79850 56810 Tricetin O-malonylhexoside 64220 79800 60230 111500 104600 156100 24780 29120 45160 38850 31640 44520 Phytocassane C 104200 116200 97050 153300 216200 197400 86930 55530 75940 71240 109400 126700 Petunidin 3-O-glucoside 817300 825000 447800 1235000 1147000 766800 453900 917300 387300 458000 362600 590000 Icariside B2 14060 19360 50390 25230 37210 36450 15160 14440 5212 7314 19660 13150 Oleoylethanolamide 22410 38870 17280 14890 14200 22290 23480 34190 25360 41260 25460 46850 2-Linoleoylglycerol 19130 28500 28050 59140 20480 69420 15260 14240 8111 15190 10740 24550 Isohyperoside 38270 41810 32160 54380 79430 70730 33000 27740 24860 43440 19120 15050 N6-(2-Hydroxyethyl)adenosine 53030 152700 48460 37080 133800 105700 149100 141000 156100 203100 207000 111900 Turanose 6727000 4476000 3339000 15480000 13030000 5498000 2398000 5056000 3373000 4360000 2085000 4526000 L-Leucyl-L-phenylalanine 44020 65060 42910 42780 42420 51960 62820 59320 80050 62500 53780 97180 Elemol 16260000 18560000 17410000 18740000 15620000 21620000 17750000 12650000 15230000 12340000 11460000 12230000 Luteolin-4'-O-glucoside 113622 194000 130756 182600 270600 288500 141900 63480 124400 77690 123900 124700 L-Glycyl-L-phenylalanine 212400 403500 186800 195600 296800 267900 268400 447800 419300 342000 329200 432800 Kukoamine A 22000 10030 18290 37160 14460 31200 11204 13550 10170 8977 12930 9517 Genistein 27100000 25280000 24260000 16390000 36550000 37020000 18950000 18410000 17590000 17770000 15960000 19830000 Esculin 73980 104200 67310 126100 81590 88800 52330 30910 61980 36790 44090 26490 Luteolin 69670 77740 69950 126500 145200 154800 47700 21820 73670 36510 59180 53230 Diosmetin 1303000 2183000 3104000 4108000 5233000 6153000 603900 124700 467300 304700 1217000 308500 p-Coumaric Acid 41710 70590 25650 23010 45050 61850 10470 6637 18030 10400 20290 6404 Raffinose 30630 34710 36020 22900 66940 48680 15770 17010 9861 30810 21030 42840 Vanillic acid 12360 11570 8206 8495 52420 11870 5531 7824 8409 6215 3393 5427 Psoralidin 56890 173200 46810 23900 211600 221600 13340 3521 59200 780 17160 21700 Sebacic acid 39120 46780 49040 43130 48440 46380 34770 38390 42380 37230 37760 29900 Isovitexin 26160 13110 14380 9169 10640 11646 10710 5250 4336 8960 3678 6446 Scutellarein 42610 58140 57800 55130 66770 82290 26830 24300 40830 26980 27090 22460 Tectorigenin 2720 7229 8806 14630 19660 22510 2063 3199 1647 5585 3900 5701 Engeletin 33710 40600 46480 31460 71320 59810 17910 13260 19970 30160 9641 26170 Calceolarioside B 52110 41010 52460 76590 50560 82960 22730 22760 31080 32880 26560 19200 Neobavaisoflavone 671100 739400 225800 379300 510300 562400 104200 88460 210800 22480 256100 326600 alpha,alpha-Trehalose 30080 30420 17030 319100 462300 64980 4297 5698 4625 6164 8152 26030 Sesamoside 8126 26140 25470 27000 29850 30310 7584 4534 5411 7738 10310 4589 L-Glutamine 96680 121400 78310 225100 110000 149700 110100 65270 56760 38660 127600 67860 L-Arabinitol 80980 75090 66190 82260 98040 52920 46350 48360 52320 56030 45740 53030 N-Acetylornithine 71950 85870 45030 83550 60200 95060 40000 41690 35700 31610 35400 30180 O-Acetylserine 7476 7378 7379 6168 8796 7069 4578 4151 5589 6605 5031 4441 12-OH-JA-Ile-1 14400 4973 4827 5541 7222 5837 3847 3568 3474 4279 3669 2914 GA7-1 4997000 3413000 4243000 7283000 6034000 5985000 1527000 1974000 2131000 2034000 1939000 4017000 Kaempferol7-O-beta-D-glucopyranoside 77510 117100 65180 113200 204000 158600 59950 22980 65830 49260 64370 67790 Sakuranetin 29790 46930 64330 58700 51790 58880 34430 26340 32090 23340 37300 52540 Riboflavin-5'-monophosphate 27480 28620 39250 133200 33510 31250 14330 16070 38070 17800 20440 19140 9(S)-HOTrE 109700 74970 73710 73360 68410 71970 32240 21840 40310 35380 27440 45100 Sinapoyl malate 6279 6929 8012 8796 17320 14720 5713 5129 5489 6146 4142 6624 alpha-Benzylsuccinic acid 128600 145000 129900 172600 121800 138500 112200 113600 124600 84050 113900 111200 beta-D-Lactose 22150 25620 16810 173800 146200 38900 5361 10820 3367 11830 7390 18050 gamma,gamma-Dimethylallyl pyrophosphate 217300 226400 93150 228800 132300 82080 51730 47220 112900 56790 59030 95620 4-Hydroxy-2-Oxoglutaric Acid 738000 928800 1059000 906000 735700 1150000 365600 408700 332100 570400 541400 553200 5Z,8Z,11Z,14Z-Eicosatetraenoic acid 11190 15920 11730 13588 27840 33850 12299 9239 11220 11686 10339 11421 dATP 8028 17230 15140 12060 12290 22900 6164 4411 13790 2878 10810 10960 3-(2-Naphthyl)-D-Alanine 11280 5585 6023 10840 4645 6310 4695 4730 3576 2769 4656 5234 D-Fructose 6-Phosphate 2076000 1004000 2096000 2051000 929600 2946000 937300 1540000 1087000 741200 637200 401300 D-Glucopyranose 274000 407000 233500 422600 403300 263500 161100 212200 225400 204000 255000 155900 Mevalonic acid 289700 296500 289700 299400 293000 289100 265700 262200 254700 249700 243400 143400 N-Acetyl-D-glucosamine 6-phosphate 7587 13090 4141 6225 5161 10290 5419 2238 4240 5045 3064 5233 N-Acetylphenylalanine 56610 119000 77340 88230 124200 107900 55350 62160 64500 38480 55950 94960 UDP 1429000 2033000 2599000 1488000 1284000 1480000 838100 1184000 975200 835000 1313000 754500 Ligustrazine 194000 154200 171500 210300 196700 221300 136000 137400 115800 132300 145600 234000 Echinacoside 7875 5174 17350 5934 14441 5976 2129 4308 5404 5297 4788 4569 Methyl protocatechuate 97430 131400 73890 128000 144400 238400 51200 23960 44430 44650 66060 43240 Methyl 4-hydroxycinnamate 5601 13440 10770 24700 71390 55380 3526 6209 2790 9654 8298 17640 D-Panthotenate 208000 210300 152600 165100 184100 103200 74050 122900 130500 113500 94970 84300 Melibiose 86220 74180 102900 1268000 1113000 336700 14800 21230 34950 46880 39790 202600 Maltose 10970 9185 12830 60400 51860 16430 6528 4976 12080 6117 6468 13030 Isovanillic acid 28190 14260 11053 11022 89790 28260 14070 7631 7726 7415 8504 9539 4'-Hydroxypropiophenone 113000 143600 119500 82910 136100 154600 73270 81820 114100 107900 84600 87230 Propylparaben 5530 16700 8998 20650 13950 20370 11838 2217 7421 2107 4741 2625 Calycosin 10000000 13270000 16440000 20210000 28670000 33970000 1730000 427000 3309000 1053000 6845000 1322000 Shogaol 393600 402400 373800 402100 400600 375700 319900 337000 325000 310800 344400 292400 Hastatoside 13960 13150 22730 49370 25020 41750 5193 3320 9396 8030 9108 5420 Naringenin chalcone 229600 237200 164700 193800 296400 281700 159100 114500 229500 122300 140400 218200 Eriodictyol 13420 13310 14700 7802 8770 17250 6503 3765 11460 4981 12850 6914 Choline bitartrate 715200 848900 885600 737700 1118000 682400 456800 533700 581000 520900 603400 808600 D-Ribose 237200 384800 399000 420300 556800 259300 216100 274800 231000 295000 170000 332100 p-Hydroxy-cinnamic acid 14940 88190 37060 33160 74990 42890 14820 18293 18440 14450 13010 27540 Apigenin 4-O-rhamnoside 4983 6083 6260 10870 13480 14870 4984 3929 4456 2096 2739 3658 Apigenin 5-O-glucoside 254600 144900 133600 129300 173800 164500 118600 106800 92660 140700 60860 102700 Apigenin 2160000 2113000 2048000 1614000 3070000 3067000 1780000 1478000 1405000 1592000 1445000 1684000 Fisetin 143600 154200 177200 98000 131900 272100 90990 89450 111000 85360 88790 89880 Genistin 195300 186700 137900 130500 110600 184300 126300 68730 65130 133600 85650 86000 Glycitein 15460 16130 18730 11870 12448 13095 11370 8618 8199 9900 16660 11120 Kaempferol 20050 23420 23460 21580 47700 33890 15500 13170 23190 18110 14390 14230 Peonidin chloride 16210 18090 19660 15590 15030 17930 16000 11130 14590 15630 13240 9664 Puerarin 334700 147200 195800 169000 334700 308600 135400 92870 158100 91870 131100 142500 Luteolin 7-O-glucoside 179700 131700 200700 200100 213800 259400 73400 101500 108200 95200 74520 120700 Galactinol 222300 132400 223200 2516000 1541000 659400 17230 51490 33780 64990 39080 491400 Acetyl tryptophan 52560 69340 62810 106700 136900 105300 30600 26550 58460 21210 36080 54320 Myristoleic acid 66340 60090 69940 383000 98870 118600 48500 21190 38450 16850 51440 62470 9,10-EODE 11400 10090 8180 9903 8511 9191 5869 1975 4124 5965 2926 3329 13(S)-HOTrE 202400 175300 161800 171800 185100 202000 111300 67610 165300 114500 109700 174900 9-KODE 226300 181800 165100 160800 189100 197600 132000 69690 157600 115900 103300 179400 9(S)-HpOTrE 91670 117500 80910 87620 86410 86080 50290 50870 64620 57770 52190 62070 12,13-EODE 1533000 1192000 1138000 1281000 1191000 1390000 531400 312300 620100 464000 483400 670800 13-HPODE 45170 53040 37030 41230 41140 45630 30640 32050 35140 28570 33740 27130 Gallic acid O-Hexoside 101000 45220 137800 44850 152800 120700 17000 8057 22280 5861 23890 43570 Pelargonidin O-acetylhexoside 600500 449200 421500 489300 1053000 1096000 204500 170000 237000 300300 174300 183600 Acetyl-eriodictyol O-hexoside 31440 22060 35520 29260 28870 39680 22650 11270 23290 14420 18340 17180 O-p-coumaroyl-O-salicyloyl quinic acid 19350 26570 5298 16970 14370 22440 4702 3968 3918 6531 5771 3329 5-O-p-coumaroyl quinic acid O-hexoside 7899 7410 7863 7848 5363 14290 5160 2404 4377 2802 4691 8480 Salicylic acid O-glucoside 206000 192200 238700 230200 369000 225300 105000 158200 177400 133100 105200 144500 Di-O-methylquercetin 3976000 4209000 3465000 5737000 4259000 4568000 2187000 2176000 2653000 2416000 2801000 3033000 Sucrose 37660 27870 63370 709500 484500 200200 15360 9562 13920 16310 13180 69510 Indole-5-carboxylic acid 637400 739000 695400 726000 338500 869500 297100 300800 376500 328700 357300 518800 Kaempferol 3-O-robinobioside 23300 21120 51330 28980 14320 29190 11620 12850 16123 8775 10820 12357 Isohemiphloin 31190 45760 28940 8135 23930 34020 11730 5180 26650 10320 10430 10630 Dihydrokaempferol 50400 30400 30990 20770 39770 47570 30740 13540 26640 27520 16310 23090 Coniferin 140500 91940 138100 1672000 1670000 548400 14510 31120 26620 43220 36210 214200 2'-Hydroxydaidzein 1077000 1234000 703000 1243000 1894000 1916000 553300 264700 837300 676900 899400 458800 Trifolin 140300 120900 107300 132100 121000 144500 50530 36780 48900 51640 46860 55060 2'-Hydroxygenistein 1362000 1827000 1808000 2833000 3350000 3873000 962600 321500 1320000 727500 995600 844600 Afzelechin 127500 166900 207900 189400 156200 227900 92990 116000 111400 118400 142100 172600 Prunetin 614900 496900 711700 1007000 998500 1797000 152800 170800 247300 214200 281400 188400 Chrysophanol 8-O-glucoside 37900 16540 14650 12580 31530 27080 10940 10960 13070 9103 13100 16220 Rhein 8-Glucoside 17490 14900 16630 20710 16450 33280 12710 7438 13310 9406 10150 8659 Isomucronulatol-7-O-glucoside 12110 10030 10960 21300 8207 23600 7248 7246 7101 5751 5411 8110 Medicarpin 459800 245100 191200 227900 606100 914000 110400 90810 114900 96120 138200 170700 Pinoresinol Diglucoside 39120 40840 44470 81700 113000 73840 27920 17480 17120 42570 17810 41950 Crocetin 62110 45540 45920 71240 60950 49030 24700 19680 45350 42230 31590 32520 Coumestrol 57700000 52000000 43270000 63530000 60470000 71340000 37530000 20930000 47600000 40830000 40080000 32410000 Purpureaside C 15190 4645 12940 3901 21600 7562 3760 3233 5828 5786 3884 3808 1,7-Dimethylxanthine 99530 118200 90400 105700 87390 90110 84630 76720 83120 72800 84310 67270 2-Deoxyribose 1-Phosphate 19500 21950 16570 21240 17190 41670 11990 14640 22490 16880 11880 11530 D-3-Phosphoglyceric acid 343400 769400 178200 300900 217900 328200 77330 84080 198000 63880 126400 211300 D-Mannose 6-phosphate 175600 89660 120300 140000 51470 81760 44960 102800 89650 45140 25070 27290 D-Xylulose 6033000 5531000 5949000 6886000 8330000 5585000 4511000 4796000 3076000 4637000 4852000 5283000 Heptamethoxyflavone 222000 332600 259900 142100 428100 453200 165900 134800 172500 142100 110600 125800 N-[(Tert-butoxy)carbonyl]-L-tryptophan 8693 12100 11840 11220 9951 9522 8805 8729 8703 7005 9505 6848 P-Coumaryl Alcohol 1480000 1272000 1806000 1719000 2344000 1333000 1267000 1348000 816400 1214000 1084000 1559000 Physcion 8-O-Beta-D-Monoglucoside 159700 176000 285100 383500 160200 691100 46280 12200 68250 14020 60390 21150 N-Acetyl-L-glutamic acid 21070 13520 20970 14460 25770 17530 10700 8581 14510 10840 6043 7969 D-Glucose 1-phosphate 577000 540000 400500 287600 398900 282800 224100 311100 346400 259400 250500 209800 Succinic anhydride 4984000 7020000 7191000 10440000 6492000 11070000 5331000 3525000 5078000 4226000 2966000 4851000 3-O-p-coumaroyl shikimic acid O-hexoside 14650 10280 7411 8356 6819 14860 4656 6942 9292 6434 6682 5488 2-Oxoadipic acid 93580 145100 121400 141800 63810 116400 38630 36180 47590 58500 55290 44620 8,15-DiHETE 10700 11920 11990 17440 23260 22660 12016 9487 11733 14400 7930 9794 N alpha-Acetyl-L-glutamine 871300 1194000 625300 1536000 1341000 1634000 457300 629800 761700 577700 515800 792000 p-Coumaric acid-4-O-glucoside 15810 8912 9972 16960 8368 18220 3681 3433 6701 4921 3153 5728 6'-O-Feruloyl-D-sucrose 135100 280000 261200 274700 156400 431400 81240 110000 105400 174400 188000 106000 Isomaltulose 119400 110400 170200 1436000 1559000 519900 15020 32400 42800 61710 28930 248400 9S-Hydroxy-10E,12Z-octadecadienoic acid 2657000 2352000 2067000 2839000 2449000 2964000 1829000 1130000 2175000 1851000 1857000 2666000 Sieboldin 24700 15670 9070 13340 23100 54090 7977 5090 6641 7456 12760 10070 1-O-Gentisoyl-D-glucoside 189600 78410 187000 89450 244500 148600 55190 42490 80080 27710 54640 148800 9,10,13-Trihydroxyoctadec-11-enoic acid 5539000 6095000 5018000 7761000 6747000 6660000 3874000 4158000 4961000 4626000 5155000 5600000 Koaburaside 72760 67110 100900 91400 120300 158900 28200 11750 47270 28040 62760 32310 Ayapin 55600 59540 39340 99220 65340 80250 23370 35050 27350 27230 23650 42440 Eucommiol 215000 366200 207600 503000 426300 407400 159600 195000 180200 145700 158000 210800 1-O-Galloyl-D-glucose 36090 26130 28470 28620 64110 50040 9294 10000 23740 15330 21470 26360 Dimethylmalonic acid 1080000 688600 978300 704600 1024000 755000 500100 823500 604000 721700 538100 813000 L-Citramalic acid 133700 58510 152300 185900 142884 113300 37840 50940 62290 69720 61160 92580 2,6-Dimethyl-7-octene-2,3,6-triol 224900 391000 240100 418200 426200 433500 199200 204300 217800 169700 204800 202600 N-Acetyl-L-Tryptophan 252100 311000 398700 481700 558500 416700 142400 93500 272000 154900 180900 252400 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name KEGG ID log2(GH3_3/CK) P-value VIP Cytidine C00475 0.91 0.015478 1.459612612 Azelaic acid C08261 -0.85 0.004468 1.622783808 trans-Zeatin riboside C16431 0.4 0.033438 1.357063344 Methyl linoleate _ 0.41 0.011574 1.506101902 SDMA _ 0.44 0.045462 1.310820544 5-Methylcytidine _ 0.84 0.0037478 1.673943452 Calycosin-7-O-beta-D-glucoside _ -2.06 0.00056027 1.849742698 Schaftoside C10181 -0.74 0.028846 1.406632949 Corylin _ -1.89 0.0095525 1.507961214 Uracil C00106 0.38 0.0093519 1.492835281 DL-Citrulline _ 0.85 0.0096579 1.550503701 L-arginine C00062 0.75 0.013604 1.592344114 Glycyl-L-leucine C02155 0.4 0.026748 1.400920253 delta-Hexanolactone _ -1.08 0.00077651 1.780582683 Sattabacin _ -1.41 0.00013914 1.90287992 Nicotinate ribonucleoside _ -0.51 0.045887 1.301291143 2'-O-methyladenosine C04779 0.63 0.017241 1.495062954 N-Acetyl-L-leucine C02710 -0.28 0.038769 1.390441373 N-Acetyl-L-histidine C02997 -0.51 0.031801 1.379281525 iP9G _ -0.65 0.033746 1.369955741 Indole-3-acetamide C02693 0.9 0.002053 1.728565902 1-Monopalmitin _ -0.56 0.0033949 1.642924211 Eriodictyol-7-O-glucoside _ -0.68 0.027306 1.393507946 dUDP C01346 -1.34 7.45E-05 1.914421449 10-Formyl-THF C00234 -0.44 0.039069 1.30740258 Tetraethylammonium fluoride _ 0.87 0.0024191 1.68953598 1-Methylhistidine C01152 1.25 0.021834 1.419196061 S-Adenosyl-methionine _ 0.91 0.010787 1.520127569 Pro-Leu _ -0.7 0.020247 1.437303324 N2-Acetyl-L-lysine C12989 -0.8 9.14E-05 1.910672422 L-Alanyl-L-leucine _ 0.4 0.02865 1.389008718 Gly-Tyr _ 0.63 0.0013584 1.775723129 Gly-Phe _ 0.51 0.0071449 1.617775587 Dl-Norleucine _ -0.34 0.046818 1.287872091 2-(Dimethylamino)Guanosine _ 0.77 0.029847 1.396804987 methyl oxo pentanoate C00233 -0.69 0.0020296 1.658627251 Trimethylamine N-oxide C01104 0.68 0.047888 1.335273443 D-Myo-inositol 4-monophosohate _ 0.49 0.034644 1.231030601 2-Deoxy-D-galactose C02781 -0.73 0.00033059 1.851309994 dAMP C00360 -0.9 0.012803 1.570311303 1-Methylguanosine C04545 0.73 0.017433 1.451511321 Scopine _ -0.53 0.0070608 1.623464024 Stachydrine C10172 -1.72 0.014626 1.48423568 Cephalotaxine C10580 -1.36 0.0042209 1.653487419 7-Ethylcamptothecin _ -3.47 0.0021362 1.699630702 Berberine C00757 -1.62 0.038385 1.302445703 Aristolone _ -1.02 0.036945 1.336436762 Homoplantaginin C17762 -1.67 0.0049096 1.635609707 Tabersonine C09244 -1.89 0.0059704 1.610700596 Aloperine C10748 0.52 0.022944 1.333014912 3-Hydroxy-9,10-Dimethoxypterocarpan _ -1.28 0.03608 1.320748994 Indirubin _ 1.43 0.019969 1.449267969 Homoharringtonine _ -0.92 0.014753 1.436156203 Pratensein-7-O-glucoside _ -1.43 0.0043203 1.61489423 Prim-O-glucosylcimifugin _ -1.9 0.0033269 1.632728356 Vincosamide _ 1.01 0.0038027 1.600176349 Oxysophocarpine _ 1.3 0.042162 1.353802191 ladanein _ -2.54 0.00824 1.505435233 5-O-p-Coumaroyl shikimic acid _ -0.87 0.031583 1.34475718 Phellodensin F _ -1.45 0.039832 1.250313094 Cocamidopropyl betaine _ -0.98 0.018981 1.440838404 MAG (18 1) _ 1.16 0.03642 1.369552216 Asp-phe _ 0.57 0.005698 1.593128483 Diethyl glutarate _ -0.85 0.00017232 1.879559767 DL-threitol _ -0.5 0.0095491 1.495692767 Methionine sulfoxide C02989 0.93 0.046859 1.18190406 Sissotrin C05376 -2.19 0.0014618 1.749644813 Peonidin O-hexoside _ -2.21 0.0005061 1.801685941 Cyanidin 3-O-malonylhexoside _ -2.73 8.68E-05 1.945560029 Pelargonidin 3-O-malonylhexoside _ -1.18 0.0064329 1.578848487 Delphinidin 3-O-Rutinoside C16315 -0.75 0.02877 1.281123988 (-)-Norepinephrine N-hexoside _ -1.17 0.00036209 1.876707447 18-Nor-4,15-dihydroxyabieta-8,11,13-trien-7-one _ -1.45 0.0080149 1.53083776 1-Methyladenine C02216 0.61 0.013137 1.567112526 2-Methyladenosine _ 0.8 0.0019057 1.778174228 Apigenin O-malonylhexoside _ -0.93 0.014597 1.454062964 Apo-13-zeaxanthinone _ -0.58 0.025883 1.417914299 Chrysoeriol 5-O-hexoside _ -1.56 0.0057469 1.580932595 D-Ala-D-Ala C00993 0.53 0.0041418 1.663257016 D-Sorbitol C00794 -0.53 0.018371 1.474026714 Glu-Glu C01425 -0.66 0.018026 1.518396707 GDP-L-fucose C00325 -1.32 0.015187 1.505616184 IAA-Asp _ -0.71 0.023513 1.416068461 IAA-Glu _ -0.94 0.010913 1.52850801 Kaempferol 3-O-glucoside-2‘-O-rhamnoside _ -1.38 0.0079555 1.622752645 Luteolin O-malonylhexoside _ -2.6 2.70E-05 1.988612536 methylApigenin C-pentoside _ -0.79 0.0008287 1.764957497 methylQuercetin O-hexoside _ -0.48 0.049033 1.226893321 N8-Acetylspermidine C01029 -0.51 0.0060637 1.551099467 N-Acetyl-5-hydroxytryptamine C00978 1.13 0.025419 1.278249859 Naringenin O-malonylhexoside _ -1.01 0.0085108 1.527162937 N-Sinapoylagmatine _ 0.6 0.048083 1.275405877 N alpha-Acetyl-L-Arginine _ -0.57 0.033252 1.310542623 Quercetin-3,4'-O-di-beta-glucopyranoside _ -1.49 5.49E-05 1.9756455 Quercetin 5-O-hexoside _ -0.78 0.045626 1.291051397 Selgin 5-O-hexoside _ -0.66 0.045517 1.325052047 S-Sulfo-L-cysteine C05824 0.6 0.011943 1.492536294 Tricin O-malonylhexoside _ -1.03 0.01468 1.447743117 Yamogenin C08918 -0.49 0.041859 1.238915238 4-Hydroxybenzoate C00156 -0.59 0.035067 1.340984748 Fustin C01378 -0.55 0.044172 1.309077967 N-Sinapoyl agmatine _ 0.94 0.020032 1.439623871 Chrysoeriol O-malonylhexoside _ -2.04 0.0011161 1.7642776 beta-Alanine C00099 0.66 0.011588 1.558648954 Histamine C00388 0.6 0.024475 1.38807809 Auramine C19193 -0.37 0.031719 1.387769219 Benzyl cinnamate _ 1.01 0.017558 1.391527395 Maltol C11918 0.51 0.040589 1.335753636 sn-Glycero-3-phosphocholine C00670 -1.02 0.0025612 1.729018563 Pyridoxine O-glucoside _ -1.19 0.0020067 1.749899412 methylLuteolin C-hexoside _ -0.83 0.032613 1.336921787 Chrysoeriol 7-O-hexoside _ -1.63 0.0028659 1.672271311 Tricetin O-malonylhexoside _ -1.42 0.00031619 1.881449969 Phytocassane C _ -0.75 0.020041 1.395249086 Petunidin 3-O-glucoside C12139 -0.72 0.031699 1.305465622 Icariside B2 _ -1.28 0.0097495 1.436452636 Oleoylethanolamide C20792 0.59 0.044367 1.309585194 2-Linoleoylglycerol _ -1.35 0.0090171 1.518707538 Isohyperoside _ -0.95 0.010469 1.510000731 N6-(2-Hydroxyethyl)adenosine _ 0.86 0.019987 1.479382239 Turanose C19636 -1.15 0.035521 1.342502032 L-Leucyl-L-phenylalanine _ 0.52 0.010866 1.487642234 Elemol _ -0.4 0.0059817 1.619498424 Luteolin-4'-O-glucoside _ -0.84 0.018079 1.421999623 L-Glycyl-L-phenylalanine _ 0.51 0.025448 1.402458147 Kukoamine A C17615 -1 0.015119 1.50672624 Genistein C09126 -0.61 0.011077 1.470346447 Esculin C09264 -1.1 0.00066857 1.836397274 Luteolin C01514 -1.14 0.0062945 1.628039734 Diosmetin C10038 -2.86 0.00032652 1.841919854 p-Coumaric Acid C06738 -1.89 0.00062397 1.797499098 Raffinose C00492 -0.8 0.041511 1.191420426 Vanillic acid C06672 -1.51 0.022568 1.391678514 Psoralidin C10523 -2.66 0.01241 1.501968111 Sebacic acid C08277 -0.3 0.004418 1.632806623 Isovitexin C01714 -1.11 0.0061832 1.569635691 Scutellarein C10184 -1.1 9.48E-05 1.943623086 Tectorigenin C10534 -1.77 0.015006 1.41337178 Engeletin _ -1.27 0.0018481 1.720874685 Calceolarioside B _ -1.19 0.00015518 1.939004532 Neobavaisoflavone _ -1.61 0.011512 1.502415502 alpha,alpha-Trehalose C01083 -4.07 0.00394 1.632944339 Sesamoside _ -1.87 0.00045518 1.785863875 L-Glutamine C00064 -0.74 0.044906 1.230594569 L-Arabinitol C00532 -0.59 0.0015958 1.736065788 N-Acetylornithine C00437 -1.04 0.00021886 1.865989739 O-Acetylserine C00979 -0.54 0.0010333 1.774353316 12-OH-JA-Ile-1 _ -0.97 0.0063851 1.601294162 GA7-1 _ -1.23 0.00052006 1.847168977 Kaempferol7-O-beta-D-glucopyranoside _ -1.15 0.0090018 1.540042048 Sakuranetin C09833 -0.59 0.02866 1.332625954 Riboflavin-5'-monophosphate _ -1.22 0.030508 1.433117323 9(S)-HOTrE C16326 -1.22 5.32E-05 1.982098039 Sinapoyl malate C02887 -0.9 0.01161 1.496161688 alpha-Benzylsuccinic acid C09816 -0.34 0.0096441 1.564245108 beta-D-Lactose C01970 -2.89 0.0048059 1.591136528 gamma,gamma-Dimethylallyl pyrophosphate C00235 -1.21 0.0070158 1.622566262 4-Hydroxy-2-Oxoglutaric Acid C01127 -0.99 0.00019129 1.841173597 5Z,8Z,11Z,14Z-Eicosatetraenoic acid _ -0.78 0.041895 1.253019129 dATP C00131 -0.83 0.043023 1.29423981 3-(2-Naphthyl)-D-Alanine _ -0.8 0.015663 1.478183471 D-Fructose 6-Phosphate C00085 -1.05 0.020387 1.465496533 D-Glucopyranose _ -0.72 0.0044089 1.613275007 Mevalonic acid C00418 -0.3 0.036292 1.307010672 N-Acetyl-D-glucosamine 6-phosphate C00357 -0.88 0.028748 1.347186516 N-Acetylphenylalanine _ -0.62 0.029097 1.310278138 UDP C00015 -0.8 0.003008 1.628546177 Ligustrazine _ -0.34 0.043091 1.254726622 Echinacoside C10450 -1.09 0.020653 1.424308549 Methyl protocatechuate _ -1.57 0.00049766 1.811800414 Methyl 4-hydroxycinnamate _ -1.91 0.042207 1.219927007 D-Panthotenate C00864 -0.72 0.0058089 1.610218861 Melibiose C05402 -3.04 0.015556 1.476312326 Maltose C00208 -1.71 0.026079 1.42357343 Isovanillic acid _ -1.73 0.023118 1.357634906 4'-Hydroxypropiophenone _ -0.44 0.024243 1.387790899 Propylparaben _ -1.47 0.0097266 1.537231667 Calycosin C01562 -3.06 0.00024835 1.875793012 Shogaol C10494 -0.28 3.25E-05 1.944275755 Hastatoside _ -2.03 0.00064589 1.847784645 Naringenin chalcone C06561 -0.51 0.032795 1.372654938 Eriodictyol C05631 -0.69 0.042482 1.302432584 Choline bitartrate C00919 -0.5 0.0084671 1.51426621 D-Ribose C21057 -0.57 0.043409 1.240785575 p-Hydroxy-cinnamic acid C00811 -1.45 0.010615 1.456544821 Apigenin 4-O-rhamnoside _ -1.37 0.0028913 1.68590575 Apigenin 5-O-glucoside _ -0.68 0.011179 1.518621764 Apigenin C01477 -0.58 0.0057831 1.546938605 Fisetin C10041 -0.81 0.004807 1.620872329 Genistin _ -0.74 0.0067304 1.534867342 Glycitein C14536 -0.41 0.037887 1.211142361 Kaempferol C05903 -0.78 0.0093543 1.555747955 Peonidin chloride C08726 -0.35 0.019292 1.430602301 Puerarin C10524 -0.98 0.0044107 1.663476737 Luteolin 7-O-glucoside C03951 -1.04 0.00015825 1.930210386 Galactinol C01235 -2.92 0.0090416 1.549065035 Acetyl tryptophan C03137 -1.23 0.0030737 1.701409299 Myristoleic acid C08322 -1.73 0.01336 1.512146966 9,10-EODE _ -1.24 0.00045025 1.849629894 13(S)-HOTrE C16316 -0.56 0.013465 1.547270774 9-KODE C14766 -0.56 0.015004 1.518200938 9(S)-HpOTrE C16321 -0.7 3.73E-05 1.96459396 12,13-EODE _ -1.32 1.34E-05 2.025953273 13-HPODE C04717 -0.49 0.00036143 1.836897331 Gallic acid O-Hexoside _ -2.31 0.0011073 1.767759923 Pelargonidin O-acetylhexoside _ -1.69 0.00019922 1.889856079 Acetyl-eriodictyol O-hexoside _ -0.8 0.0021981 1.75577726 O-p-coumaroyl-O-salicyloyl quinic acid _ -1.89 0.00071286 1.773301143 5-O-p-coumaroyl quinic acid O-hexoside _ -0.86 0.018009 1.449339599 Salicylic acid O-glucoside _ -0.82 0.0012602 1.782315161 Di-O-methylquercetin _ -0.78 0.00011494 1.928800712 Sucrose C00089 -3.46 0.0096787 1.546832906 Indole-5-carboxylic acid _ -0.87 0.0040548 1.659605027 Kaempferol 3-O-robinobioside _ -1.21 0.0022266 1.740798318 Isohemiphloin _ -1.19 0.027994 1.378430192 Dihydrokaempferol C00974 -0.67 0.034124 1.350724995 Coniferin C00761 -3.54 0.0062473 1.594732304 2'-Hydroxydaidzein C02495 -1.12 0.0074755 1.583501811 Trifolin C12626 -1.4 1.06E-07 2.099898457 2'-Hydroxygenistein C12134 -1.54 0.0018984 1.747617235 Afzelechin C09320 -0.51 0.015254 1.439564604 Prunetin C10521 -2.16 4.38E-05 1.970324212 Chrysophanol 8-O-glucoside _ -0.93 0.016544 1.452032001 Rhein 8-Glucoside _ -0.95 0.0016191 1.779134453 Isomucronulatol-7-O-glucoside _ -1.07 0.0044122 1.673074967 Medicarpin C10503 -1.87 0.0016838 1.721530449 Pinoresinol Diglucoside _ -1.25 0.0063314 1.567895415 Crocetin C08588 -0.77 0.0038363 1.723505511 Coumestrol C10205 -0.66 0.0055422 1.658589724 Purpureaside C C10488 -1.32 0.028226 1.376162009 1,7-Dimethylxanthine C13747 -0.33 0.0036488 1.638072513 2-Deoxyribose 1-Phosphate C00672 -0.62 0.039599 1.360428559 D-3-Phosphoglyceric acid C00197 -1.49 0.0056242 1.610234234 D-Mannose 6-phosphate C00275 -0.97 0.031769 1.409291928 D-Xylulose C00310 -0.49 0.0061689 1.505136826 Heptamethoxyflavone _ -1.1 0.0041866 1.606396667 N-[(Tert-butoxy)carbonyl]-L-tryptophan _ -0.35 0.0099697 1.485913904 P-Coumaryl Alcohol C02646 -0.44 0.037749 1.228457517 Physcion 8-O-Beta-D-Monoglucoside C10384 -3.06 0.00023453 1.901642699 N-Acetyl-L-glutamic acid C00624 -0.95 0.0017847 1.775745422 D-Glucose 1-phosphate C00103 -0.63 0.016643 1.451110216 Succinic anhydride C19524 -0.86 0.0037949 1.683875807 3-O-p-coumaroyl shikimic acid O-hexoside _ -0.65 0.027679 1.422586331 2-Oxoadipic acid C00322 -1.28 0.00016229 1.893815014 8,15-DiHETE _ -0.58 0.047184 1.276690339 N alpha-Acetyl-L-glutamine _ -0.94 0.0050256 1.640155618 p-Coumaric acid-4-O-glucoside _ -1.5 0.00028938 1.931874369 6'-O-Feruloyl-D-sucrose _ -1 0.011975 1.469726426 Isomaltulose C01742 -3.18 0.0077153 1.577966538 9S-Hydroxy-10E,12Z-octadecadienoic acid _ -0.41 0.035348 1.374273877 Sieboldin _ -1.48 0.0097577 1.510361859 1-O-Gentisoyl-D-glucoside _ -1.19 0.013288 1.505621012 9,10,13-Trihydroxyoctadec-11-enoic acid _ -0.41 0.0071267 1.593231344 Koaburaside _ -1.53 0.0016145 1.753596729 Ayapin C18078 -1.15 0.00066297 1.81220978 Eucommiol C17878 -1.01 0.002301 1.689301295 1-O-Galloyl-D-glucose _ -1.13 0.0062617 1.615500622 Dimethylmalonic acid _ -0.38 0.048223 1.245854854 L-Citramalic acid C02614 -1.07 0.0055027 1.65392355 2,6-Dimethyl-7-octene-2,3,6-triol _ -0.83 0.0017813 1.704878339 N-Acetyl-L-Tryptophan _ -1.14 0.0021359 1.747546571 METABOLITES_END #END