#METABOLOMICS WORKBENCH yxw_524521_20240927_100018 DATATRACK_ID:5240 STUDY_ID:ST003538 ANALYSIS_ID:AN005810 PROJECT_ID:PR002177 VERSION 1 CREATED_ON October 17, 2024, 10:59 pm #PROJECT PR:PROJECT_TITLE Metabolomic analysis of fluorescent hairy roots overexpressing the Gretchen PR:PROJECT_TITLE Hagen 3 genes enhancing soybean resistance to cyst nematodes PR:PROJECT_SUMMARY Soybean root samples were collected, target metabolites were extracted, standard PR:PROJECT_SUMMARY curves were established, methodology verification was performed, LC-MS/MS PR:PROJECT_SUMMARY metabolites were detected, and data were analyzed.Metabolome analysis was PR:PROJECT_SUMMARY conducted on FHRs overexpressing these genes to investigate how plant hormones PR:PROJECT_SUMMARY play a role in soybean resistance to SCN. These results provide a new framework PR:PROJECT_SUMMARY for understanding the interaction between soybean and SCN. PR:INSTITUTE Shenyang Agricultural University PR:LAST_NAME yang PR:FIRST_NAME wen PR:ADDRESS College of Plant Protection PR:EMAIL 2020200130@stu.syau.edu.cn PR:PHONE 15702428682 #STUDY ST:STUDY_TITLE Metabolomic analysis of fluorescent hairy roots overexpressing the Gretchen ST:STUDY_TITLE Hagen 3_2 genes enhancing soybean resistance to cyst nematodes ST:STUDY_SUMMARY The Gretchen Hagen 3 genes maintain endogenous hormone homeostasis by ST:STUDY_SUMMARY conjugating excess hormones with amino acids. Herein, we identified the members ST:STUDY_SUMMARY of the GH3 family in soybeans and analyzed their phylogeny, gene duplication, ST:STUDY_SUMMARY structure, domains, conserved motifs, cis-elements in promoter regions for ST:STUDY_SUMMARY stress responses, and functional characteristics. We found that GH3 genes are ST:STUDY_SUMMARY induced by pathogens in Group-II. Furthermore, 8 out of 16 Group-II genes ST:STUDY_SUMMARY responded to cyst nematode infection. Through functional analysis of eight GmGH3 ST:STUDY_SUMMARY genes via overexpression, they play a negative role in soybean resistance to ST:STUDY_SUMMARY cyst nematode. In addition, our metabolomic analysis showed that overexpression ST:STUDY_SUMMARY of Glyma.02G125600, Glyma.17G165500, and Glyma.13G284600 affected the content of ST:STUDY_SUMMARY salicylic acid and jasmonic acid. Our findings clarify the functional features ST:STUDY_SUMMARY of GH3 genes and reveal their involvement in plant hormone signaling pathways. ST:STUDY_SUMMARY This provides valuable insights into the complex molecular mechanisms underlying ST:STUDY_SUMMARY the interaction between soybeans and cyst nematode. ST:INSTITUTE Shenyang Agricultural University ST:LAST_NAME Yang ST:FIRST_NAME Xiaowen ST:ADDRESS College of Plant Protection ST:EMAIL 2020200130@stu.syau.edu.cn ST:PHONE 15702428682 #SUBJECT SU:SUBJECT_TYPE Plant SU:SUBJECT_SPECIES Glycine max SU:TAXONOMY_ID 3847 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - CK-1 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-2 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-3 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-4 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-5 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - CK-6 Sample source:Plant root | Phenotype:control RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_2-1 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_2-2 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_2-3 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_2-4 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_2-5 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- SUBJECT_SAMPLE_FACTORS - GH3_2-6 Sample source:Plant root | Phenotype:transgene RAW_FILE_NAME(Raw file name)=- #COLLECTION CO:COLLECTION_SUMMARY Liquid nitrogen grinding samples, weighing a certain amount of samples into mass CO:COLLECTION_SUMMARY spectrometry water, vortex mixing, as diluted samples; Take a dilution sample of CO:COLLECTION_SUMMARY 100μL and add 400 μL precipitator (acetonitrile: Water =1:1), vortex mixing, CO:COLLECTION_SUMMARY extraction at 4 ° C for 30 min, centrifugation at 12000 rpm and 4 ° C for 10 CO:COLLECTION_SUMMARY min, taking 300μL supernatant slowly through the extraction column, adding 500 CO:COLLECTION_SUMMARY μL eluent (30% acetonitrile), slowly through the extraction column, mixing the CO:COLLECTION_SUMMARY two times of flow solution, LC-MS analysis. CO:SAMPLE_TYPE Plant roots #TREATMENT TR:TREATMENT_SUMMARY Take a dilution sample of 100μL and add 400 μL precipitator (acetonitrile: TR:TREATMENT_SUMMARY Water =1:1), vortex mixing, extraction at 4 ° C for 30 min, centrifugation at TR:TREATMENT_SUMMARY 12000 rpm and 4 ° C for 10 min, taking 300μL supernatant slowly through the TR:TREATMENT_SUMMARY extraction column, adding 500 μL eluent (30% acetonitrile), slowly through the TR:TREATMENT_SUMMARY extraction column, mixing the two times of flow solution, LC-MS analysis. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The concentration series of the standard solution were detected by LC-MS, and SP:SAMPLEPREP_SUMMARY the ratio of the concentration of the standard product to the internal standard SP:SAMPLEPREP_SUMMARY was taken as the horizontal coordinate and the ratio of the peak area of the SP:SAMPLEPREP_SUMMARY standard product to the internal standard was taken as the vertical coordinate SP:SAMPLEPREP_SUMMARY to investigate the linearity of the standard solution. QC samples were tested by SP:SAMPLEPREP_SUMMARY inserting a number of needles at intervals in each batch of samples. The RSD of SP:SAMPLEPREP_SUMMARY all QC samples was calculated with the concentration of the standard substance SP:SAMPLEPREP_SUMMARY to evaluate stability. According to the established sample pretreatment and SP:SAMPLEPREP_SUMMARY instrumental analysis methods, all samples were quantitatively analyzed. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME AB Sciex QTRAP 6500+ CH:COLUMN_NAME Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) CH:SOLVENT_A 5mM Ammonium Acetate Aqueous Solution (Adjusted to pH 4.5 with Acetic Acid) CH:SOLVENT_B Acetonitrile CH:FLOW_GRADIENT The initial proportion of mobile phase B is set at 15%, which then linearly CH:FLOW_GRADIENT increases at a rate of 0.8% per minute until it reaches 45%, where it is held CH:FLOW_GRADIENT for 5 minutes to adequately elute moderately polar metabolites. Afterward, it CH:FLOW_GRADIENT increases at a rate of 2% per minute up to 90%, and finally remains at this CH:FLOW_GRADIENT composition for an additional 5 minutes to clean the chromatographic column and CH:FLOW_GRADIENT collect less polar metabolites. CH:FLOW_RATE 0.35ml/min CH:COLUMN_TEMPERATURE 35 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME AB Sciex QTRAP 6500+ MS:INSTRUMENT_TYPE QTRAP MS:MS_TYPE EI MS:ION_MODE POSITIVE MS:MS_COMMENTS The concentration series of the standard solution were detected by LC-MS, and MS:MS_COMMENTS the ratio of the concentration of the standard product to the internal standard MS:MS_COMMENTS was taken as the horizontal coordinate and the ratio of the peak area of the MS:MS_COMMENTS standard product to the internal standard was taken as the vertical coordinate MS:MS_COMMENTS to investigate the linearity of the standard solution. QC samples were tested by MS:MS_COMMENTS inserting a number of needles at intervals in each batch of samples. The RSD of MS:MS_COMMENTS all QC samples was calculated with the concentration of the standard substance MS:MS_COMMENTS to evaluate stability. According to the established sample pretreatment and MS:MS_COMMENTS instrumental analysis methods, all samples were quantitatively analyzed. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak area MS_METABOLITE_DATA_START Samples CK-1 CK-2 CK-3 CK-4 CK-5 CK-6 GH3_2-1 GH3_2-2 GH3_2-3 GH3_2-4 GH3_2-5 GH3_2-6 Factors Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:control Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Sample source:Plant root | Phenotype:transgene Adenosine 95440000 44840000 10230000 44610000 3631000 10900000 1.33E+08 1.56E+08 1.56E+08 1.09E+08 1.36E+08 71270000 Cytidine 12980000 16550000 10360000 11630000 19200000 17860000 18030000 21100000 36660000 29620000 21290000 21220000 Indole-3-acetic acid 76270 160900 64930 53850 66490 66070 44770 32120 19010 41260 35450 18780 Ademetionine 34570 53460 24950 21050 22900 41720 62760 60600 63240 37020 34590 61680 SDMA 264900 511000 297400 189600 231100 241500 819600 640300 524400 533200 418300 349700 5-Methylcytidine 174800 269200 195000 160900 200300 231900 286400 263900 365500 318500 188800 222800 Calycosin-7-O-beta-D-glucoside 375300 377100 611600 569400 446600 1621000 191500 276000 216000 464500 299000 133100 L-Leucine 32050000 40200000 19830000 18450000 24400000 16380000 45400000 45860000 40480000 38090000 38480000 20380000 Glycine 70050 54070 39400 57630 31790 36440 147300 129300 68820 154400 118000 77620 DL-Citrulline 1907000 2725000 1184000 1200000 1630000 1014000 3161000 3811000 2968000 3650000 2843000 2484000 beta-Nicotinamide Mononucleotide 14470 10140 13730 3970 13580 7282 149500 82070 37060 29020 327100 7945 H-Gly-Pro-OH 79420 153000 86270 89150 105000 96850 133300 168500 162500 157800 99720 140100 2-Amino-4-(methylthio)butyric acid 160900 236300 185200 154400 136400 123200 429500 375900 173300 199400 232700 215800 L-serine 1028000 1378000 817300 1003000 842400 815100 2526000 2055000 1482000 1811000 1971000 1274000 D-Phenylalanine 41970000 56930000 27990000 31710000 29630000 27540000 72090000 81700000 71820000 81320000 71130000 34260000 Nicotinamide 2743000 1949000 2886000 3217000 3306000 2752000 3629000 5181000 5094000 4267000 3317000 3659000 4-Acetamidobutyric Acid 277600 295700 155000 432800 460500 215900 438300 641900 545900 399900 644700 593400 Toringin 1.28E+08 91650000 1.06E+08 48330000 88490000 1.11E+08 1.22E+08 1.33E+08 1.48E+08 1.52E+08 1.2E+08 1.09E+08 Sattabacin 42380 40250 49080 82870 56250 77640 28600 28590 27640 43640 19840 32040 1,2-Benzenedicarboxylic acid 317600 343000 175700 183400 179500 199100 427000 510100 437900 491200 473800 236100 Phe-Pro 97220 96370 26750 30490 92440 50520 153800 242800 207100 270800 115200 120400 Oleamide 53710000 33510000 49150000 37360000 61430000 56830000 1.3E+08 69030000 93050000 43610000 53720000 1.02E+08 NG,NG-Dimethyl-L-arginine 300800 426400 397800 295000 335300 189300 668600 503700 584800 422900 444000 340000 N-Acetyl-L-leucine 23500000 17960000 18650000 22140000 19670000 19720000 26880000 33080000 27600000 25700000 25900000 24370000 2-Hydroxy-6-Aminopurine 1152000 953900 555600 226600 728200 362500 1510000 2085000 597000 1831000 1656000 1074000 N-(2-Hydroxyethyl)iminodiacetic acid 1885000 1218000 2250000 3101000 2511000 2654000 1320000 1439000 1113000 1128000 1062000 1504000 Hypoxanthine-9-beta-D-Arabinofuranoside 7042000 3330000 756800 3232000 290200 768300 9574000 11230000 11000000 7275000 9966000 5011000 Diethanolamine 202200 289300 160300 230100 180300 168500 519700 443600 313100 375600 360900 291000 Benzamidine 683100 893500 417400 490900 511900 407300 1180000 1265000 1200000 1323000 1217000 554600 Acetaminophen 785600 764600 397000 322800 400700 511500 1192000 1350000 959300 1173000 1038000 757300 Tetraethylammonium fluoride 5446000 6756000 4607000 3076000 6323000 6163000 4725000 11700000 9343000 9382000 8665000 5868000 5'-Deoxy-5'-(Methylthio)Adenosine 371200 667000 479600 498100 658500 480500 3269000 2536000 1213000 1460000 1347000 2232000 gamma-Glutamylglutamic acid 258300 220000 192100 111000 330300 257700 385300 347000 401100 267700 228500 380700 1-Methylhistidine 449500 302100 123100 121700 244700 198200 999400 1229000 942700 824300 686800 716600 S-ribosyl-L-homocysteine 107900 123400 59760 73420 99830 69310 130400 158300 163700 155200 123000 117100 Spermidine 11750 19080 13270 16780 11890 23090 88780 42860 21090 52930 18680 45880 S-Methyl-5'-thioadenosine 571900 1057000 779700 780200 1019000 713900 5295000 3938000 1974000 2262000 2102000 3573000 Purine 53200 72550 40390 41620 39220 31710 89310 90470 95150 105400 95390 42800 L-Saccharopine 601200 877200 502100 515900 646400 678000 918300 824800 987700 832600 642600 778900 Homoserine 7274000 7779000 3485000 4361000 3250000 2122000 10180000 9720000 8364000 8132000 9844000 4239000 DL-Leucine 711400 915400 432800 388900 566400 364300 1010000 1026000 948500 856100 908000 453600 Asp-glu 152000 183800 143800 146400 191200 151000 213000 254700 245900 345200 171600 230200 Allopurinol 35790 48990 15390 41320 29210 23560 90960 132000 95450 263500 60200 80200 5-Methyl-dl-tryptophan 93590 93010 103900 54410 107000 116200 151000 148900 133100 113200 89550 125500 1-Methylnicotinamide 21710 18620 12700 9047 4417 12930 67410 80410 73370 157700 39450 37030 D-Pyroglutamic acid 1697000 2032000 928200 4738100 1401000 1113000 10730000 11400000 8900000 17630000 6533000 8307000 methyl oxo pentanoate 80050 129800 112400 97320 148900 137100 80260 58300 81600 98370 108700 83070 Trimethylamine N-oxide 19710 43350 21510 17140 25910 30080 56590 42000 38540 51700 21760 35390 D-Myo-inositol 4-monophosohate 5644000 4072000 4238000 5203000 2490000 4772000 5254000 5087000 6885000 6808000 6363000 5070000 3'-Aenylic Acid 91310 87940 72280 43450 63410 82440 95980 93880 94650 95270 97190 84850 dAMP 97590 65810 67830 125200 158300 135900 70170 24630 67240 55820 12230 14330 1-Methylguanosine 36830 36410 16560 13530 28920 23900 44240 41360 43270 61360 44120 51590 dCMP 39440 39150 32380 31210 25800 48260 87980 110200 74410 78390 104300 64920 thiamine phosphate 15040 11420 2591 9211 12064 5702 20290 30110 32130 21280 36200 8914 dGMP 5831 6858 4472 9969 6349 10670 18780 8444 11713 11370 18820 15640 Scopine 22750 22690 28640 31340 22590 32720 17600 21020 13550 22780 20130 24770 Quinidine 13180 9801 14770 15470 21170 13680 15090 23150 25470 24180 22060 14190 Cephalotaxine 11133 12070 14080 8054 11980 34480 6339 9879 3228 13296 2591 8787 Deoxyarbutin 58200 112900 132000 110000 89420 63170 278300 109000 156700 121500 94060 170400 2,2'-Cyclouridine 59832 62870 44260 45611 34440 34610 89500 67250 106700 70711 102700 38520 4-(1H-Pyrazol-4-yl)-7-((2-(trimethylsilyl)ethoxy)-methyl)-7H-pyrrolo[2,3-d]pyrimidine 2655 3562 2463 3384 2350 5540 1907 2071 2322 2557 2273 949 Aloesin 7534 6921 4666 7006 6967 4120 10630 13710 8857 7287 6608 7034 Homoplantaginin 182200 127700 191400 239000 312300 548100 187400 147800 121900 112600 94220 161000 Tabersonine 242600 333800 203200 474700 601400 974100 116200 128700 84030 198700 64710 195900 Aloperine 7873 7236 9446 9516 9533 7409 17380 18410 17190 19770 9613 10630 VitaMin U 10750000 8440000 3973000 4002000 3009000 3070000 11080000 12220000 9608000 11080000 16830000 5999000 3-Amino-4-methylpentanoic acid 545300 292400 525800 494200 565000 305500 1229000 907700 696300 566600 546400 523500 D-Serine 6389000 7021000 4712000 5450000 5369000 4544000 12890000 12570000 8317000 10040000 11280000 7542000 1-Aminocyclopropanecarboxylic acid 3814000 3794000 1846000 1817000 1733000 1370000 4824000 4736000 4422000 4199000 5471000 2294000 L-Phenylalanine 40580000 56840000 26750000 29190000 28700000 26010000 69540000 79180000 70360000 78000000 66770000 31740000 Levodopa 68070 79130 79710 116600 123800 95720 271100 164500 117500 103900 108000 156000 Nobiletin 34120 34450 25350 42470 29420 50300 121200 116500 110900 147600 79820 64790 3-Hydroxy-9,10-Dimethoxypterocarpan 26070 71720 28320 43180 88870 95260 12370 27930 36090 33450 8735 40170 4-Hydroxycoumarin 25760 31140 4099 6044 14010 8567 53710 54470 41950 31080 33840 23520 Paeonol 127400 195400 139900 47430 163000 76090 223100 246000 248600 243300 237300 122900 Indirubin 9606 26930 3357 4406 5280 13540 142300 65500 41490 49670 18740 26420 Homoharringtonine 22470 44770 22090 26440 36950 32740 38340 25640 12910 16850 13940 8748 Pratensein-7-O-glucoside 89760 132100 236100 137700 294300 351100 38230 146600 111800 62570 112300 39840 Prim-O-glucosylcimifugin 113500 94080 344700 255900 290400 440200 54610 86280 52090 37650 107300 32240 Oxymatrine 64120 73000 34790 26490 41150 29010 213700 208600 106200 85970 137500 68260 Oxysophocarpine 9034 13720 4927 3295 7558 16200 84720 47370 18300 32830 28810 24450 ladanein 10970 86840 12090 39590 82270 47160 2981 14863 5341 5406 2008 11299 Sarsasapogenin 96170000 58780000 73020000 34280000 61390000 76010000 91840000 1.08E+08 1.09E+08 1.22E+08 99680000 71410000 N-Acetylthreonine 39740 60290 43170 42480 49480 40160 190500 100500 75920 66550 66420 69120 7-methylguanine 8256000 11170000 5352000 6074000 5826000 5110000 14270000 16340000 15230000 16050000 14340000 6826000 Chrysin O-hexoside 2.12E+08 1.48E+08 1.66E+08 78500000 1.51E+08 1.86E+08 2.07E+08 2.36E+08 2.37E+08 2.62E+08 2.2E+08 1.73E+08 Agmatine 105900 92660 82520 32400 59190 51950 204400 211900 178900 211000 105300 165500 Gallocatechin-gallocatechin 8287 22880 28320 8753 24620 36800 6104 7401 15100 11910 5730 9956 LysoPC 10:0 158100 202700 56820 75230 50890 66740 257900 195100 207900 132400 154100 107100 MAG (18:1) 5289 5666 3096 2133 2676 7611 10060 5598 9090 5429 8043 6590 Daidzin 1.61E+08 1.31E+08 1.4E+08 71970000 1.22E+08 1.58E+08 1.62E+08 1.81E+08 1.83E+08 2.02E+08 1.67E+08 1.44E+08 Guanosine monophosphate 74580 116200 119100 49470 44330 78090 368700 211800 222800 175200 456000 92590 Methionine sulfoxide 1179000 682400 841000 563300 366400 324400 2077000 1715000 1515000 1165000 2105000 1014000 Methylguanidine 322700 326300 143000 142500 159600 76490 438400 409700 408200 316400 476700 205800 Sissotrin 810800 819200 1484000 1131000 1575000 3494000 142700 330700 163300 358000 291100 186800 Peonidin O-hexoside 93500 204000 256800 326600 358700 432000 44530 121500 107700 49130 76300 30340 Delphinidin 3-O-Rutinoside 6016 9929 7571 12480 9894 14718 8438 7453 4511 5332 6902 3798 (-)-Norepinephrine N-hexoside 827200 929100 1426000 1484000 1359000 1880000 665600 778000 672300 625900 653300 1027000 1-Methyladenine 82090 145000 79080 76980 77270 56430 249000 156300 156200 160100 153600 114800 2-Methyladenosine 1712000 3067000 1851000 1555000 2413000 2562000 3324000 2742000 2777000 3632000 1978000 3601000 5-Sulfanyl-1-pentanesulfonamide 66530 44310 55920 40870 69540 49630 161300 75880 106500 44270 60940 120300 Apo-13-zeaxanthinone 392400 303000 535500 564100 665500 523300 335600 449600 367600 385600 281100 307900 Chinese bittersweet alkaloid II 30970 22630 26570 28310 44560 17780 37270 43390 44980 45610 38830 34830 Chrysoeriol 5-O-hexoside 171200 127700 240700 213800 349400 668200 154300 107200 107200 89970 91060 124300 GDP-L-fucose 14390 11380 10510 26050 8458 15100 11880 5480 1570 3657 12420 9189 IAA-Asp 455000 763100 789800 747100 1168000 1001000 410000 417200 389800 436700 350600 594500 Kaempferol 3-O-glucoside-2‘-O-rhamnoside 15630 10880 17010 32970 28720 35970 7799 9968 7007 5392 3615 3347 L-Homocystine 5779000 2784000 607400 2649000 248900 649800 8178000 9618000 9147000 6286000 8202000 4218000 Luteolin O-malonylhexoside 1561000 982700 1744000 1734000 1707000 3994000 887000 1340000 1150000 1724000 605700 351000 methylChrysoeriol 5-O-hexoside 54600 34480 37600 31790 58360 47450 61280 69170 57670 61040 48460 65190 methylQuercetin O-hexoside 639300 477700 329100 536900 636900 433300 732700 594400 741200 533900 697800 1275000 N-(9H-Purin-6-ylcarbamoyl)threonine 137000 95220 125700 90880 137700 144700 353000 199400 295600 132900 170100 257200 N,N-Caffeoyl, p-cinnamoylputrescine 8032 7545 3758 8434 11860 8799 24830 33090 15730 8475 14580 20560 N-p-Coumaroylspermidine 12970 15050 14410 18300 20600 14610 9437 11120 13690 10630 7748 16200 Pyridoxine 24470 38330 20780 41020 39450 43000 39240 48110 56940 44870 38690 57200 Xanthurenic acid O-hexoside 22308 2707 25481 29144 1499 28999 87050 51780 58690 109800 55320 68770 Linoleic acid 64040 38170 57070 38370 47160 50600 166800 89550 126100 41250 84650 112300 Selgin O-hexosyl-O-hexoside 190000 121300 97230 46970 41900 46440 113900 192400 226800 146600 154500 89730 N-Sinapoyl agmatine 11430 6428 12340 2087 9157 10780 22740 22780 19480 14620 15390 12950 Chrysoeriol O-malonylhexoside 275500 192500 405400 521900 648700 1206000 193500 163300 179900 204500 106600 137800 Orotic acid 307600 242200 248800 440800 463197 314200 1415000 855200 461000 411300 470200 1122000 L-Dihydroorotic acid 16680000 15530000 14920000 11640000 11670000 15350000 16810000 26550000 20190000 17960000 17350000 14150000 D-Threonine 1614000 1275000 694200 707000 676000 391900 1792000 1975000 1812000 1484000 2325000 820300 Gossypol 10791 11750 11491 11572 11797 14181 12070 19460 15864 17870 14400 14780 Auramine 123500 119100 91090 149400 106700 109500 101500 84740 73660 111700 85430 84050 Benzyl cinnamate 15300 19980 24950 12210 15880 18910 34140 47840 63910 62980 44190 21510 Vanillin 43230 24500 49520 75990 49890 29790 30225 23000 14960 25450 20240 28460 Nerol 6603 16410 6069 8678 13400 10970 15557 15690 27880 21740 15260 8917 N-p-Coumaroylputrescine 7170 11540 4535 7367 8137 17200 34170 32660 21490 28180 20830 17980 D-Threose 40000 56060 240610 23610 31140 47020 483000 630600 421800 965600 373000 521200 Phytocassane C 104200 116200 97050 153300 216200 197400 101400 113200 101400 106500 86870 84690 1,5-Diaminopentane 101000 67350 39050 6985 41910 39990 143500 101300 131100 203300 75480 85300 Icariside B2 14060 19360 50390 25230 37210 36450 14120 16400 18610 13940 13500 18190 HBOA 29860000 43470000 20210000 22100000 21690000 19380000 52320000 59850000 52550000 57720000 51640000 23420000 Isomangiferin 125900 122200 126900 205200 26870 180800 1015000 592400 304900 231000 360100 747300 L-Alanyl-L-Phenylalanine 73780 111800 44330 49160 37050 40070 75900 95980 122600 102900 87340 65710 L-Leucyl-L-phenylalanine 44020 65060 42910 42780 42420 51960 61460 75740 79170 98940 63360 62600 Elemol 16260000 18560000 17410000 18740000 15620000 21620000 14480000 10590000 16390000 15810000 14990000 14610000 Phenylethanolamine 36030 4948 3436 5598 8573 28850 21290 50130 32150 63770 33560 27730 D-Panthenol 9249 5553 7784 16770 9812 12390 1388000 1467000 881000 2091000 685600 848100 Luteolin 69670 77740 69950 126500 145200 154800 28550 42960 76590 43860 37780 47740 Diosmetin 1303000 2183000 3104000 4108000 5233000 6153000 372900 245100 188800 475000 202800 594900 Quinic acid 1470000 849200 2092000 2584000 198900 1398000 8265000 2521000 3615000 2066000 2129000 7034000 DL-Panthenol 5715 3157 21626 6756 4168 28479 240200 213500 177700 448300 128300 158200 p-Coumaric Acid 41710 70590 25650 23010 45050 61850 11100 24900 15520 38540 7680 23180 3,4-Dihydroxyphenylacetic acid 46110 37480 52120 60490 75290 48120 28490 38360 37340 44540 19850 42150 Tartaric acid 326000 387600 482800 506500 623500 346200 163700 211300 312700 222200 148500 190400 Scutellarein 42610 58140 57800 55130 66770 82290 27040 32700 53320 33090 25160 28720 Tectorigenin 2720 7229 8806 14630 19660 22510 1261 4537 2814 3476 4177 3195 Engeletin 33710 40600 46480 31460 71320 59810 37510 20220 36200 29740 30100 31050 Isoguanosine 908300 878400 574900 432800 542900 629900 1346000 1029000 1293000 1205000 971800 755000 Sesamoside 8126 26140 25470 27000 29850 30310 6875 2458 4418 6502 8038 4107 Uridine 5'-monophosphate 115800 55460 66110 70050 23540 27880 212000 147200 236700 137300 222100 63460 L-Threonine 171800 151200 93690 151900 85080 38770 154200 361700 277200 170400 387200 147800 Cytosine 8818 6211 9118 9014 8454 6799 17820 16060 10970 15690 16760 12240 cis-Aconitic acid 196800 119000 227900 486500 88810 232400 3363000 1205000 1037000 212300 451200 1569000 Citric acid 1601000 1935000 3855000 3362000 467000 3212000 25880000 6002000 11190000 2035000 4052000 16310000 L-Pyroglutamic acid 10520 5925 5886 9160 4749 4817 21650 29010 33900 46010 21590 22600 L-Arabinitol 80980 75090 66190 82260 98040 52920 35530 15340 38100 44800 33210 24730 N-Acetylornithine 71950 85870 45030 83550 60200 95060 60360 44140 54060 53140 34890 59940 D-Galacturonic acid 153500 78340 201400 230300 30010 207000 812400 253000 399500 208700 262600 821900 GA7-1 4997000 3413000 4243000 7283000 6034000 5985000 3365000 949000 2774000 3476000 1500000 2227000 3-Hydroxyanthranilic Acid 38540 36180 40600 43740 93750 49990 22990 22700 25990 26340 13320 55340 Lactic acid 24310 80576 33480 18720 19080 16760 106400 98700 236900 405500 112800 160700 9(S)-HOTrE 109700 74970 73710 73360 68410 71970 62320 26310 67750 40280 34690 48980 alpha-Benzylsuccinic acid 128600 145000 129900 172600 121800 138500 139000 69080 116500 96160 89030 139400 ADP-ribose 13220 13678 14840 8075 9009 10730 119000 26610 25040 13050 36430 11550 Citraconic acid 100000 72940 127500 228300 30770 67590 926800 229000 363500 111300 144300 381000 deoxyribose-phosphate 27650 24830 18470 15020 31810 12240 42440 23870 31530 29650 32950 34510 D-Glucarate 1734000 2808000 2169000 1883000 2081000 1854000 2196000 2459000 2903000 5509000 4000000 2459000 D-Glucopyranose 274000 407000 233500 422600 403300 263500 131400 230200 252400 242800 203200 140800 L-Cysteinesulfinic acid 4969 10810 9323 10391 10270 10939 13900 16890 19250 21050 19740 7948 D-threo-Isocitric acid 3696000 1451000 3507000 4056000 286800 2819000 11880000 5751000 7256000 2747000 4862000 12390000 L-Homoserine 3265 2695 1648 6877 2981 6299 25740 8753 3242 7437 7016 6872 D-Arabinose 5-phosphate 238100 198300 240600 137300 115700 57080 317300 340700 295300 189300 409800 172600 UDP-galactose 1017000 151800 678600 522000 55190 108900 1521000 1435000 1992000 578300 1641000 949900 Methyl protocatechuate 97430 131400 73890 128000 144400 238400 21410 48730 47710 106400 30720 74980 Oxyresveratrol 197900 529000 220500 349800 228900 331700 251900 207400 202600 182000 163800 160600 Vidarabine 225600 77760 35130 117200 9139 20600 281500 231600 287900 177600 299200 118500 Propylparaben 5530 16700 8998 20650 13950 20370 46570 30200 19520 23990 21570 31350 Hexadecanedioic acid 14390 20790 18640 18380 16060 13000 14570 14020 15030 11750 10850 11530 Calycosin 10000000 13270000 16440000 20210000 28670000 33970000 1294000 1574000 1163000 2557000 1809000 2736000 beta-L-Arabinose 3779000 5497000 6042000 5479000 8553000 4996000 1899000 1732000 2435000 2572000 2117000 2247000 Hastatoside 13960 13150 22730 49370 25020 41750 11820 5804 4616 10830 7212 12560 Choline bitartrate 715200 848900 885600 737700 1118000 682400 408700 399400 376900 493500 346700 383500 Cytidylic acid 27100 50410 34570 23820 15410 24020 61760 45610 91260 55180 72570 34790 2-Amino-3-hydroxypropionic acid 218400 237400 218600 144000 93850 49080 396600 260900 340500 171600 368400 242000 D-Ribose 237200 384800 399000 420300 556800 259300 184000 198800 209500 233100 137500 139400 Peonidin chloride 16210 18090 19660 15590 15030 17930 11110 15130 12840 13340 13780 11610 Acetyl tryptophan 52560 69340 62810 106700 136900 105300 37870 26230 47800 42960 28090 55250 Myristoleic acid 66340 60090 69940 383000 98870 118600 27690 24680 45250 29570 30620 53250 13(S)-HOTrE 202400 175300 161800 171800 185100 202000 138000 113100 172500 116300 107100 138800 9-KODE 226300 181800 165100 160800 189100 197600 159900 112000 161600 128900 101200 128300 12,13-EODE 1533000 1192000 1138000 1281000 1191000 1390000 933300 408300 1033000 725900 479200 728900 UDP-D-glucose 898000 140700 419500 335700 47220 69880 1332000 738700 1290000 359400 1215000 465500 Gallic acid O-Hexoside 101000 45220 137800 44850 152800 120700 6883 17970 22290 77490 14440 15190 Di-O-methylquercetin 3976000 4209000 3465000 5737000 4259000 4568000 3750000 2116000 3451000 4082000 2007000 2880000 L(-)-Malic acid 34650000 25990000 43090000 37600000 28490000 33730000 70040000 34570000 47320000 41920000 46590000 66870000 N-[(-)-Jasmonoyl]-(L)-Isoleucine 5824 4585 4546 3109 5053 4893 11500 6901 10760 11670 6340 5031 5-oxoproline 21860 5258 7279 10790 12250 4513 31940 39380 48780 61880 32810 22760 2'-Hydroxydaidzein 1077000 1234000 703000 1243000 1894000 1916000 271800 275800 890000 818700 273700 649300 Trifolin 140300 120900 107300 132100 121000 144500 106800 59430 93740 71160 48630 80760 2'-Hydroxygenistein 1362000 1827000 1808000 2833000 3350000 3873000 559500 725700 1263000 940800 576000 799600 Prunetin 614900 496900 711700 1007000 998500 1797000 207000 269400 337900 507700 213500 267800 D-Xylose 53560 46580 95460 82310 104300 59690 40220 25390 46820 42510 37780 29510 Fmoc-Ile-OH 35010 30980 38170 35500 8051 28290 3619 3781 9102 7374 10679 8813 Rhein 8-Glucoside 17490 14900 16630 20710 16450 33280 16650 7818 8050 12940 11290 11330 4'-Hydroxyacetophenone 6784 2717 12780 6079 10330 7082 50870 26320 21910 53320 20740 26100 Pinoresinol Diglucoside 39120 40840 44470 81700 113000 73840 26370 28730 29710 30620 11770 46360 Crocetin 62110 45540 45920 71240 60950 49030 47740 20890 38000 51840 21860 38480 Coumestrol 57700000 52000000 43270000 63530000 60470000 71340000 26810000 36170000 52090000 49500000 34320000 47200000 D-2-Hydroxypentanedioic acid disodium salt 235100 105200 235900 298000 35040 283100 1235000 446300 588600 219500 252200 1042000 D-Xylulose 6033000 5531000 5949000 6886000 8330000 5585000 1942000 2111000 2586000 2149000 1877000 2051000 Isocitrate 4326000 1633000 4847000 6188000 664600 8462000 16600000 6960000 12430000 5013000 7432000 22320000 P-Coumaryl Alcohol 1480000 1272000 1806000 1719000 2344000 1333000 505800 665900 778800 713400 575600 640900 Physcion 8-O-Beta-D-Monoglucoside 159700 176000 285100 383500 160200 691100 67930 85930 102700 146800 54990 19780 Cyclic AMP 137700 184100 222300 91820 97620 111900 616300 246000 272100 163200 342900 137200 2–furanoic acid 385600 111700 475500 636700 49210 546500 1461000 855700 971900 274500 546100 1794000 Fumaric Acid 1295000 909600 1352000 1156000 812100 1353000 2156000 1324000 1426000 1267000 1349000 1979000 2-Oxoadipic acid 93580 145100 121400 141800 63810 116400 56970 55700 112800 79890 68000 81250 N alpha-Acetyl-L-glutamine 871300 1194000 625300 1536000 1341000 1634000 724100 656700 582000 847400 420700 530900 13(S)-HODE;13(S)-Hydroxyoctadeca-9Z,11E-dienoic acid 1618000 1392000 1290000 1583000 1455000 1878000 1255000 971100 1491000 1048000 754400 1067000 (-)-Jasmonoyl-L-isoleucine 2115 3308 2445 8338 3051 7050 16120 11550 16350 14460 13100 6422 9S-Hydroxy-10E,12Z-octadecadienoic acid 2657000 2352000 2067000 2839000 2449000 2964000 2015000 1781000 2350000 1696000 1330000 1928000 D-Arabinono-1,4-lactone 26270 32390 30010 52030 19540 52360 195600 107000 63830 29000 41090 125400 9,10,13-Trihydroxyoctadec-11-enoic acid 5539000 6095000 5018000 7761000 6747000 6660000 5894000 4194000 5347000 5805000 3194000 4253000 UDP-D-xylose 167000 33430 56890 60320 11960 8818 379900 175600 152600 53670 323000 151600 Koaburaside 72760 67110 100900 91400 120300 158900 17810 20460 23780 38110 24070 30100 Ayapin 55600 59540 39340 99220 65340 80250 46500 25770 34390 37140 24070 25420 Eucommiol 215000 366200 207600 503000 426300 407400 246000 185600 176900 221800 127800 161100 1-O-Galloyl-D-glucose 36090 26130 28470 28620 64110 50040 10730 10620 14000 15090 11580 22940 2,2-Dimethylsuccinic acid 68550 88630 79980 95450 117800 129300 265400 279600 110200 133000 134800 126700 L-Citramalic acid 133700 58510 152300 185900 142884 113300 488500 233500 303700 115500 162600 627400 12(13)-EpOME 2791000 2216000 2121000 2620000 2378000 2952000 2181000 1665000 2414000 1780000 1323000 1774000 2,6-Dimethyl-7-octene-2,3,6-triol 224900 391000 240100 418200 426200 433500 291100 203800 189700 179900 145200 170000 N-Acetyl-L-Tryptophan 252100 311000 398700 481700 558500 416700 214000 175200 193800 183100 128100 199300 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name log2(GH3_2/CK) P-value VIP Adenosine 1.86 0.005987 1.607178 Cytidine 0.73 0.006659 1.561119 Indole-3-acetic acid -1.35 0.001927 1.709407 Ademetionine 0.68 0.024876 1.483948 SDMA 0.92 0.005509 1.663813 5-Methylcytidine 0.41 0.047245 1.275903 Calycosin-7-O-beta-D-glucoside -1.34 0.013019 1.543096 L-Leucine 0.59 0.043985 1.305939 Glycine 1.26 0.000872 1.809119 DL-Citrulline 0.96 0.001348 1.797739 beta-Nicotinamide Mononucleotide 3.32 0.011338 1.508 H-Gly-Pro-OH 0.49 0.016484 1.514005 2-Amino-4-(methylthio)butyric acid 0.7 0.027574 1.463266 L-serine 0.91 0.000663 1.841753 D-Phenylalanine 0.93 0.00463 1.635105 Nicotinamide 0.57 0.004305 1.648428 4-Acetamidobutyric Acid 0.82 0.007544 1.587921 Toringin 0.44 0.041975 1.30986 Sattabacin -0.95 0.002565 1.712199 1,2-Benzenedicarboxylic acid 0.88 0.004529 1.645149 Phe-Pro 1.49 0.002845 1.734647 Oleamide 0.74 0.035494 1.393092 NG,NG-Dimethyl-L-arginine 0.6 0.018908 1.44245 N-Acetyl-L-leucine 0.42 0.000622 1.863234 2-Hydroxy-6-Aminopurine 1.13 0.021146 1.483171 N-(2-Hydroxyethyl)iminodiacetic acid -0.84 0.003663 1.644888 Hypoxanthine-9-beta-D-Arabinofuranoside 1.8 0.006483 1.598443 Diethanolamine 0.9 0.000554 1.8667 Benzamidine 0.98 0.003849 1.651269 Acetaminophen 1.02 0.0014 1.80464 Tetraethylammonium fluoride 0.61 0.046033 1.287216 5'-Deoxy-5'-(Methylthio)Adenosine 1.93 3.90E-05 2.03589 gamma-Glutamylglutamic acid 0.55 0.042912 1.37349 1-Methylhistidine 1.9 0.000102 1.984884 S-ribosyl-L-homocysteine 0.66 0.003877 1.688591 Spermidine 1.49 0.005606 1.678699 S-Methyl-5'-thioadenosine 1.95 4.00E-05 2.033297 Purine 0.89 0.006156 1.576876 L-Saccharopine 0.38 0.023547 1.4538 Homoserine 0.83 0.02515 1.392952 DL-Leucine 0.62 0.041931 1.311862 Asp-glu 0.59 0.004045 1.670771 Allopurinol 1.89 0.000895 1.805365 5-Methyl-dl-tryptophan 0.42 0.049791 1.353302 1-Methylnicotinamide 2.51 0.000271 1.882377 D-Pyroglutamic acid 2.41 6.68E-05 1.947296 methyl oxo pentanoate -0.46 0.032335 1.421937 Trimethylamine N-oxide 0.64 0.043236 1.351781 D-Myo-inositol 4-monophosohate 0.42 0.038217 1.234331 3'-Aenylic Acid 0.34 0.040386 1.335496 dAMP -1.41 0.008686 1.565442 1-Methylguanosine 0.87 0.004183 1.681205 dCMP 1.26 2.93E-05 2.004203 thiamine phosphate 1.41 0.012226 1.501082 dGMP 0.94 0.005739 1.575037 Scopine -0.42 0.02436 1.387378 Quinidine 0.49 0.039596 1.237344 Cephalotaxine -1.05 0.04265 1.207555 Deoxyarbutin 0.71 0.046792 1.274663 2,2'-Cyclouridine 0.75 0.023626 1.344035 4-(1H-Pyrazol-4-yl)-7-((2-(trimethylsilyl)ethoxy)-methyl)-7H-pyrrolo[2,3-d]pyrimidine -0.72 0.028946 1.410453 Aloesin 0.54 0.040475 1.390048 Homoplantaginin -0.95 0.031686 1.293401 Tabersonine -1.84 0.002454 1.672814 Aloperine 0.86 0.00192 1.739856 VitaMin U 1 0.013111 1.495481 3-Amino-4-methylpentanoic acid 0.71 0.027305 1.422731 D-Serine 0.9 0.000275 1.911418 1-Aminocyclopropanecarboxylic acid 0.85 0.014881 1.479327 L-Phenylalanine 0.92 0.006601 1.59435 Levodopa 0.7 0.027153 1.448595 Nobiletin 1.56 4.97E-05 1.963491 3-Hydroxy-9,10-Dimethoxypterocarpan -1.15 0.041794 1.251279 4-Hydroxycoumarin 1.41 0.007334 1.640953 Paeonol 0.81 0.024894 1.403154 Indirubin 2.44 0.002381 1.774053 Homoharringtonine -0.67 0.047768 1.189422 Pratensein-7-O-glucoside -1.27 0.017925 1.464923 Prim-O-glucosylcimifugin -2.05 0.0015 1.772424 Oxymatrine 1.61 0.00154 1.808581 Oxysophocarpine 2.11 0.00124 1.836684 ladanein -2.73 0.003529 1.605248 Sarsasapogenin 0.58 0.021078 1.429716 N-Acetylthreonine 1.04 0.004646 1.703853 7-methylguanine 0.99 0.003738 1.657959 Chrysin O-hexoside 0.5 0.030455 1.373283 Agmatine 1.34 0.000895 1.850705 Gallocatechin-gallocatechin -1.2 0.031623 1.359023 LysoPC 10:0 0.78 0.035784 1.364096 MAG (18:1) 0.75 0.025316 1.408709 Daidzin 0.4 0.040113 1.319887 Guanosine monophosphate 1.66 0.003265 1.648691 Methionine sulfoxide 1.27 0.002408 1.707534 Methylguanidine 0.94 0.016675 1.467172 Sissotrin -2.66 7.22E-05 1.957478 Peonidin O-hexoside -1.96 0.001445 1.771904 Delphinidin 3-O-Rutinoside -0.73 0.020389 1.411564 (-)-Norepinephrine N-hexoside -0.83 0.003532 1.653942 1-Methyladenine 0.93 0.002062 1.737261 2-Methyladenosine 0.45 0.046855 1.327647 5-Sulfanyl-1-pentanesulfonamide 0.8 0.046548 1.343018 Apo-13-zeaxanthinone -0.48 0.039807 1.290007 Chinese bittersweet alkaloid II 0.51 0.014437 1.46865 Chrysoeriol 5-O-hexoside -1.39 0.0081 1.541997 GDP-L-fucose -0.95 0.048965 1.23118 IAA-Asp -0.92 0.002222 1.695518 Kaempferol 3-O-glucoside-2‘-O-rhamnoside -1.92 0.000541 1.812336 L-Homocystine 1.84 0.005809 1.613797 Luteolin O-malonylhexoside -0.95 0.044366 1.289721 methylChrysoeriol 5-O-hexoside 0.45 0.014624 1.563353 methylQuercetin O-hexoside 0.58 0.036136 1.358734 N-(9H-Purin-6-ylcarbamoyl)threonine 0.94 0.004607 1.687002 N,N-Caffeoyl, p-cinnamoylputrescine 1.27 0.006533 1.69369 N-p-Coumaroylspermidine -0.47 0.021592 1.38862 Pyridoxine 0.46 0.038227 1.322526 Xanthurenic acid O-hexoside 1.96 0.008733 1.523868 Linoleic acid 1.07 0.009813 1.59032 Selgin O-hexosyl-O-hexoside 0.76 0.048811 1.261231 N-Sinapoyl agmatine 1.04 0.015977 1.518865 Chrysoeriol O-malonylhexoside -1.72 0.004244 1.649327 Orotic acid 1.23 0.00971 1.628774 L-Dihydroorotic acid 0.39 0.032486 1.397952 D-Threonine 0.92 0.020145 1.42582 Gossypol 0.39 0.006507 1.610062 Auramine -0.37 0.019041 1.445227 Benzyl cinnamate 1.35 0.000986 1.746174 Vanillin -0.93 0.010913 1.520575 Nerol 0.75 0.040332 1.260473 N-p-Coumaroylputrescine 1.47 0.000514 1.876779 D-Threose 2.95 6.78E-05 1.951624 Phytocassane C -0.57 0.034949 1.306652 1,5-Diaminopentane 1.32 0.019598 1.470605 Icariside B2 -0.94 0.016892 1.470579 HBOA 0.92 0.007339 1.579101 Isomangiferin 2.04 0.003365 1.714518 L-Alanyl-L-Phenylalanine 0.62 0.029433 1.352238 L-Leucyl-L-phenylalanine 0.6 0.002243 1.703854 Elemol -0.31 0.018392 1.54228 Phenylethanolamine 1.38 0.013013 1.521609 D-Panthenol 6.9 1.59E-09 2.146201 Luteolin -1.21 0.002162 1.744632 Diosmetin -3.4 1.80E-05 2.00028 Quinic acid 1.57 0.025257 1.394867 DL-Panthenol 4.28 1.26E-05 2.006581 p-Coumaric Acid -1.14 0.01856 1.379465 3,4-Dihydroxyphenylacetic acid -0.6 0.022287 1.385458 Tartaric acid -1.09 0.000421 1.872625 Scutellarein -0.85 0.00153 1.768396 Tectorigenin -1.95 0.008243 1.588214 Engeletin -0.61 0.029766 1.384668 Isoguanosine 0.73 0.005111 1.641038 Sesamoside -2.18 0.000258 1.939094 Uridine 5'-monophosphate 1.5 0.006128 1.562534 L-Threonine 1.11 0.019715 1.416241 Cytosine 0.88 0.000148 1.911746 cis-Aconitic acid 2.53 0.007168 1.63474 Citric acid 2.18 0.020354 1.45566 L-Pyroglutamic acid 2.08 1.32E-05 1.99642 L-Arabinitol -1.24 0.000516 1.907865 N-Acetylornithine -0.52 0.0323 1.3001 D-Galacturonic acid 1.61 0.01717 1.461438 GA7-1 -1.16 0.004201 1.70255 3-Hydroxyanthranilic Acid -0.86 0.023052 1.354905 Lactic acid 2.53 0.000292 1.845308 9(S)-HOTrE -0.75 0.006345 1.617686 alpha-Benzylsuccinic acid -0.36 0.048318 1.284173 ADP-ribose 1.73 0.032299 1.367737 Citraconic acid 1.78 0.019033 1.459317 deoxyribose-phosphate 0.58 0.025729 1.400632 D-Glucarate 0.64 0.031668 1.247078 D-Glucopyranose -0.73 0.008697 1.596225 L-Cysteinesulfinic acid 0.8 0.019203 1.361483 D-threo-Isocitric acid 1.5 0.03065 1.366086 L-Homoserine 1.31 0.041073 1.340797 D-Arabinose 5-phosphate 0.8 0.041194 1.286405 UDP-galactose 1.67 0.011615 1.498398 Methyl protocatechuate -1.3 0.006836 1.59057 Oxyresveratrol -0.66 0.031876 1.318277 Vidarabine 1.52 0.01347 1.478872 Propylparaben 1 0.012641 1.536974 Hexadecanedioic acid -0.38 0.017836 1.429517 Calycosin -3.46 1.63E-06 2.07396 beta-L-Arabinose -1.4 1.77E-05 2.037276 Hastatoside -1.65 0.00296 1.701228 Choline bitartrate -1.05 1.06E-05 2.017652 Cytidylic acid 1.04 0.006036 1.550573 2-Amino-3-hydroxypropionic acid 0.88 0.031431 1.340975 D-Ribose -1.03 0.001344 1.775966 Peonidin chloride -0.39 0.001279 1.7693 Acetyl tryptophan -1.16 0.002299 1.732196 Myristoleic acid -1.91 0.004472 1.683361 13(S)-HOTrE -0.48 0.001699 1.730027 9-KODE -0.5 0.003144 1.653634 12,13-EODE -0.84 0.002335 1.733963 UDP-D-glucose 1.49 0.021954 1.39238 Gallic acid O-Hexoside -1.96 0.002818 1.725967 Di-O-methylquercetin -0.51 0.020629 1.458469 L(-)-Malic acid 0.59 0.014868 1.460616 N-[(-)-Jasmonoyl]-(L)-Isoleucine 0.89 0.006171 1.590955 5-oxoproline 1.93 0.000426 1.831819 2'-Hydroxydaidzein -1.34 0.005033 1.658572 Trifolin -0.73 0.001737 1.713973 2'-Hydroxygenistein -1.62 0.000366 1.883977 Prunetin -1.64 0.000775 1.834964 D-Xylose -0.99 0.002412 1.784008 Fmoc-Ile-OH -2.02 0.001196 1.835092 Rhein 8-Glucoside -0.81 0.007598 1.588053 4'-Hydroxyacetophenone 2.12 0.000297 1.868548 Pinoresinol Diglucoside -1.17 0.010169 1.495242 Crocetin -0.61 0.023388 1.431515 Coumestrol -0.5 0.017395 1.488678 D-2-Hydroxypentanedioic acid disodium salt 1.66 0.026572 1.412406 D-Xylulose -1.59 6.98E-08 2.122817 Isocitrate 1.43 0.029751 1.358723 P-Coumaryl Alcohol -1.35 8.38E-06 2.044681 Physcion 8-O-Beta-D-Monoglucoside -1.95 0.004282 1.687988 Cyclic AMP 1.07 0.029115 1.366605 2–furanoic acid 1.42 0.048181 1.279944 Fumaric Acid 0.46 0.03278 1.369169 2-Oxoadipic acid -0.58 0.036753 1.392755 N alpha-Acetyl-L-glutamine -0.93 0.006351 1.577096 13(S)-HODE;13(S)-Hydroxyoctadeca-9Z,11E-dienoic acid -0.48 0.009066 1.533912 (-)-Jasmonoyl-L-isoleucine 1.56 0.001437 1.712483 9S-Hydroxy-10E,12Z-octadecadienoic acid -0.46 0.005948 1.57382 D-Arabinono-1,4-lactone 1.4 0.032155 1.431401 9,10,13-Trihydroxyoctadec-11-enoic acid -0.39 0.032006 1.343566 UDP-D-xylose 1.86 0.013753 1.49741 Koaburaside -1.98 1.25E-05 2.040177 Ayapin -1.04 0.001722 1.75106 Eucommiol -0.92 0.00682 1.574044 1-O-Galloyl-D-glucose -1.45 0.000381 1.85029 2,2-Dimethylsuccinic acid 0.85 0.017572 1.542773 L-Citramalic acid 1.29 0.029005 1.407701 12(13)-EpOME -0.43 0.011356 1.498855 2,6-Dimethyl-7-octene-2,3,6-triol -0.85 0.004079 1.613497 N-Acetyl-L-Tryptophan -1.14 0.000246 1.878943 METABOLITES_END #END