#METABOLOMICS WORKBENCH engelmab_20241021_031103 DATATRACK_ID:5297 STUDY_ID:ST003552 ANALYSIS_ID:AN005839 PROJECT_ID:PR002188
VERSION             	1
CREATED_ON             	November 6, 2024, 8:10 pm
#PROJECT
PR:PROJECT_TITLE                 	Comprehension of the age-dependent gut and brain interaction of honeybee workers
PR:PROJECT_TITLE                 	by integration of multi omics approaches
PR:PROJECT_SUMMARY               	In honeybees, division of labour is a key feature, with age-related behavioural
PR:PROJECT_SUMMARY               	transitions being closely associated with molecular changes in the brain, gut,
PR:PROJECT_SUMMARY               	and microbiota. In this study, to investigate these molecular changes and thus
PR:PROJECT_SUMMARY               	better understand their contribution to behavioural responses and modulation, we
PR:PROJECT_SUMMARY               	analysed the global metabolomic shifts in honeybee workers and their microbiota
PR:PROJECT_SUMMARY               	throughout their lives. Overall, our findings provide new insights toward
PR:PROJECT_SUMMARY               	developing potential biomarkers for evaluation of different functional changes
PR:PROJECT_SUMMARY               	related to various environmental stressors.
PR:INSTITUTE                     	Helmholtz Centre for Environmental Research
PR:DEPARTMENT                    	Molecular Systems Biology
PR:LAST_NAME                     	Engelmann
PR:FIRST_NAME                    	Beatrice
PR:ADDRESS                       	Permoserstraße 15, Leipzipg, Saxony, 03418, Germany
PR:EMAIL                         	beatrice.engelmann@ufz.de
PR:PHONE                         	004934160251099
#STUDY
ST:STUDY_TITLE                   	Investigation of age-dependent changes in the gut of honeybee workers using
ST:STUDY_TITLE                   	targeted metabolomics for SCFA determination
ST:STUDY_SUMMARY                 	To determine changes in the metabolic activity of the gut microbiota, we
ST:STUDY_SUMMARY                 	measured the concentrations of seven short-chain fatty acids (SCFA) and lactate.
ST:STUDY_SUMMARY                 	All SCFA showed low concentrations in newly emerged workers. We found that
ST:STUDY_SUMMARY                 	acetate, propionate and butyrate are the main SCFA in the honeybee gut and peak
ST:STUDY_SUMMARY                 	in 5-day old and in-hive workers. Lactate showed an inverse trend with highest
ST:STUDY_SUMMARY                 	concentrations in 1-day old workers and a significant decrease on all subsequent
ST:STUDY_SUMMARY                 	days (Kruskal Wallis test, p <0.05).
ST:INSTITUTE                     	Helmholtz Centre for Environmental Research
ST:DEPARTMENT                    	Molecular Systems Biology
ST:LAST_NAME                     	Engelmann
ST:FIRST_NAME                    	Beatrice
ST:ADDRESS                       	Permoserstraße 15, Leipzipg, Saxony, 03418, Germany
ST:EMAIL                         	beatrice.engelmann@ufz.de
ST:PHONE                         	004934160251099
#SUBJECT
SU:SUBJECT_TYPE                  	Insect
SU:SUBJECT_SPECIES               	Apis mellifera
SU:TAXONOMY_ID                   	7460
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	H1_D0_1	H1_D0_1	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_2	H1_D0_2	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_3	H1_D0_3	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_4	H1_D0_4	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_5	H1_D0_5	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_1	H2_D0_1	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_2	H2_D0_2	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_3	H2_D0_3	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_4	H2_D0_4	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_5	H2_D0_5	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_1	H3_D0_1	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_2	H3_D0_2	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_3	H3_D0_3	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_4	H3_D0_4	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_5	H3_D0_5	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_1	H1_D1_1	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_2	H1_D1_2	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_3	H1_D1_3	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_4	H1_D1_4	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_5	H1_D1_5	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_1	H2_D1_1	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_2	H2_D1_2	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_3	H2_D1_3	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_4	H2_D1_4	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_5	H2_D1_5	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_1	H3_D1_1	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_2	H3_D1_2	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_3	H3_D1_3	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_4	H3_D1_4	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_5	H3_D1_5	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_1	H1_D3_1	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_2	H1_D3_2	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_3	H1_D3_3	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_4	H1_D3_4	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_5	H1_D3_5	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_1	H2_D3_1	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_2	H2_D3_2	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_3	H2_D3_3	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_4	H2_D3_4	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_5	H2_D3_5	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_1	H3_D3_1	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_2	H3_D3_2	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_3	H3_D3_3	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_4	H3_D3_4	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_5	H3_D3_5	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_1	H1_D5_1	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_2	H1_D5_2	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_3	H1_D5_3	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240227_beegut_Eli_Set1.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_4	H1_D5_4	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_5	H1_D5_5	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_1	H2_D5_1	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_2	H2_D5_2	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_3	H2_D5_3	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_4	H2_D5_4	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_5	H2_D5_5	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_1	H3_D5_1	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_2	H3_D5_2	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_3	H3_D5_3	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_4	H3_D5_4	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_5	H3_D5_5	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_1	H1_D7_1	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_2	H1_D7_2	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_3	H1_D7_3	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_4	H1_D7_4	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_5	H1_D7_5	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_1	H2_D7_1	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_2	H2_D7_2	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_3	H2_D7_3	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_4	H2_D7_4	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_5	H2_D7_5	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_1	H3_D7_1	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_2	H3_D7_2	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_3	H3_D7_3	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_4	H3_D7_4	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_5	H3_D7_5	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H1_NF_1	H1_NF_1	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_1	H2_NF_1	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_2	H2_NF_2	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_3	H2_NF_3	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_4	H2_NF_4	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_5	H2_NF_5	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_1	H3_NF_1	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_2	H3_NF_2	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_3	H3_NF_3	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_4	H3_NF_4	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_5	H3_NF_5	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H1_EF_1	H1_EF_1	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_1	H2_EF_1	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_2	H2_EF_2	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_3	H2_EF_3	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_4	H2_EF_4	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_5	H2_EF_5	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_1	H3_EF_1	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_2	H3_EF_2	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_3	H3_EF_3	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_4	H3_EF_4	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_5	H3_EF_5	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240229_beegut_Eli_Set2.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_1	H1_D0_1_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_2	H1_D0_2_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_3	H1_D0_3_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_4	H1_D0_4_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_5	H1_D0_5_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_1	H2_D0_1_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_2	H2_D0_2_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_3	H2_D0_3_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_4	H2_D0_4_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_5	H2_D0_5_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_1	H3_D0_1_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_2	H3_D0_2_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_3	H3_D0_3_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_4	H3_D0_4_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_5	H3_D0_5_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_1	H1_D1_1_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_2	H1_D1_2_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_3	H1_D1_3_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_4	H1_D1_4_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_5	H1_D1_5_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_1	H2_D1_1_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_2	H2_D1_2_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_3	H2_D1_3_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_4	H2_D1_4_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_5	H2_D1_5_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_1	H3_D1_1_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_2	H3_D1_2_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_3	H3_D1_3_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_4	H3_D1_4_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_5	H3_D1_5_diluted	life stage:establishment | age of workers in days:1 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_1	H1_D3_1_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_2	H1_D3_2_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_3	H1_D3_3_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_4	H1_D3_4_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_5	H1_D3_5_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_1	H2_D3_1_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_2	H2_D3_2_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_3	H2_D3_3_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_4	H2_D3_4_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_5	H2_D3_5_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_1	H3_D3_1_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_2	H3_D3_2_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_3	H3_D3_3_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_4	H3_D3_4_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_5	H3_D3_5_diluted	life stage:establishment | age of workers in days:3 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_1	H1_D5_1_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_2	H1_D5_2_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_3	H1_D5_3_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240304_beegut_Eli_Set1_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_4	H1_D5_4_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_5	H1_D5_5_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_1	H2_D5_1_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_2	H2_D5_2_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_3	H2_D5_3_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_4	H2_D5_4_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_5	H2_D5_5_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_1	H3_D5_1_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_2	H3_D5_2_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_3	H3_D5_3_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_4	H3_D5_4_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_5	H3_D5_5_diluted	life stage:establishment | age of workers in days:5 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_1	H1_D7_1_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_2	H1_D7_2_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_3	H1_D7_3_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_4	H1_D7_4_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_5	H1_D7_5_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_1	H2_D7_1_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_2	H2_D7_2_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_3	H2_D7_3_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_4	H2_D7_4_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_5	H2_D7_5_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_1	H3_D7_1_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_2	H3_D7_2_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_3	H3_D7_3_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_4	H3_D7_4_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_5	H3_D7_5_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_NF_1	H1_NF_1_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_1	H2_NF_1_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_2	H2_NF_2_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_3	H2_NF_3_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_4	H2_NF_4_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_5	H2_NF_5_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_1	H3_NF_1_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_2	H3_NF_2_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_3	H3_NF_3_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_4	H3_NF_4_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_5	H3_NF_5_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H1_EF_1	H1_EF_1_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=1; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_1	H2_EF_1_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_2	H2_EF_2_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_3	H2_EF_3_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_4	H2_EF_4_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_5	H2_EF_5_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=2; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_1	H3_EF_1_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_2	H3_EF_2_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_3	H3_EF_3_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_4	H3_EF_4_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_5	H3_EF_5_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	hive=3; RAW_FILE_NAME(Raw_data)=20240306_beegut_Eli_Set2_1v50.wiff
#COLLECTION
CO:COLLECTION_SUMMARY            	Workers were pooled to obtain a final sample number of n = 5. The brain(s) and
CO:COLLECTION_SUMMARY            	gut(s) from the same workers were assigned the same sample names. In total, we
CO:COLLECTION_SUMMARY            	had 15 samples from each age group, with the exception of the foraging stages,
CO:COLLECTION_SUMMARY            	where owing to the lack of foragers from Hive 1, we only had 11. All sampled
CO:COLLECTION_SUMMARY            	bees were placed directly in liquid nitrogen to prevent changes in the metabolic
CO:COLLECTION_SUMMARY            	profile. They were then decapitated in the laboratory so that the head and body
CO:COLLECTION_SUMMARY            	of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for
CO:COLLECTION_SUMMARY            	later dissection. Worker guts were dissected on Petri dishes. Sternite 6 and the
CO:COLLECTION_SUMMARY            	stinger apparatus were gently pulled using tweezers, which allowed extraction of
CO:COLLECTION_SUMMARY            	the entire gastrointestinal tract. The stinger apparatus and venom sac were
CO:COLLECTION_SUMMARY            	carefully excised and the honey crop was removed.
CO:SAMPLE_TYPE                   	Bee gut
#TREATMENT
TR:TREATMENT_SUMMARY             	On the day of newly emerged workers (day 0), bees were sampled as a baseline for
TR:TREATMENT_SUMMARY             	no or low bacterial abundance in the gut. Moreover, bees from each hive were
TR:TREATMENT_SUMMARY             	collected on Day 1, 3 and 5 to cover the establishment phase of the gut. Samples
TR:TREATMENT_SUMMARY             	for the in-hive worker phase, defined as workers inside the hives that have a
TR:TREATMENT_SUMMARY             	fully developed microbiome, were taken every day from Day 7 to Day 10. For the
TR:TREATMENT_SUMMARY             	foraging phase, we differentiated between new and experienced foragers, as these
TR:TREATMENT_SUMMARY             	show different behaviour due to different levels of experience.Based on
TR:TREATMENT_SUMMARY             	experience, they were either collected one to two days after onset (new
TR:TREATMENT_SUMMARY             	foragers) or at least four days after onset (experienced foragers).
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	In brief, x mg pool gut was mixed with five times the volume (in µL) of
SP:SAMPLEPREP_SUMMARY            	acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30
SP:SAMPLEPREP_SUMMARY            	Hz, 10 min; Retsch Qiagen). After centrifugation (2 min, 14000 rpm) 40 µl were
SP:SAMPLEPREP_SUMMARY            	used for further derivatization of short chain fatty acids. Each sample was
SP:SAMPLEPREP_SUMMARY            	mixed with ACN to a final concentration of 50 %. SCFAs were derivatized with 200
SP:SAMPLEPREP_SUMMARY            	mM 3-nitrophenylhydrazine and 120 mM
SP:SAMPLEPREP_SUMMARY            	N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride in pyridine and then
SP:SAMPLEPREP_SUMMARY            	diluted in 10 % ACN.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Dionex Ultimate 3000 RS
CH:COLUMN_NAME                   	Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
CH:SOLVENT_A                     	100% water; 0.01% formic acid
CH:SOLVENT_B                     	100% acetonitrile; 0.01% formic acid
CH:FLOW_GRADIENT                 	0-2min: 15% B, 2-17min: 15-50% B, 17-17.1min: 50-100% B, 17.1-18min: 100% B,
CH:FLOW_GRADIENT                 	18-18.1min: 100-15% B, 18.1-21min: 15% B
CH:FLOW_RATE                     	0.35 mL/min
CH:COLUMN_TEMPERATURE            	40
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 5500 QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	For identification and quantitation, a scheduled MRM method was used, with
MS:MS_COMMENTS                   	specific transitions for every SCFA. Data acquisition and peak integration were
MS:MS_COMMENTS                   	performed in SciexOS software (Version 3.0.0.). Calculation of concentration was
MS:MS_COMMENTS                   	done using external calibration curves and statistics were performed using the R
MS:MS_COMMENTS                   	software programme.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	µMol
MS_METABOLITE_DATA_START
Samples	H1_D0_1	H1_D0_2	H1_D0_3	H1_D0_4	H1_D0_5	H2_D0_1	H2_D0_2	H2_D0_3	H2_D0_4	H2_D0_5	H3_D0_1	H3_D0_2	H3_D0_3	H3_D0_4	H3_D0_5	H1_D1_1	H1_D1_2	H1_D1_3	H1_D1_4	H1_D1_5	H2_D1_1	H2_D1_2	H2_D1_3	H2_D1_4	H2_D1_5	H3_D1_1	H3_D1_2	H3_D1_3	H3_D1_4	H3_D1_5	H1_D3_1	H1_D3_2	H1_D3_3	H1_D3_4	H1_D3_5	H2_D3_1	H2_D3_2	H2_D3_3	H2_D3_4	H2_D3_5	H3_D3_1	H3_D3_2	H3_D3_3	H3_D3_4	H3_D3_5	H1_D5_1	H1_D5_2	H1_D5_3	H1_D5_4	H1_D5_5	H2_D5_1	H2_D5_2	H2_D5_3	H2_D5_4	H2_D5_5	H3_D5_1	H3_D5_2	H3_D5_3	H3_D5_4	H3_D5_5	H1_D7_1	H1_D7_2	H1_D7_3	H1_D7_4	H1_D7_5	H2_D7_1	H2_D7_2	H2_D7_3	H2_D7_4	H2_D7_5	H3_D7_1	H3_D7_2	H3_D7_3	H3_D7_4	H3_D7_5	H1_NF_1	H2_NF_1	H2_NF_2	H2_NF_3	H2_NF_4	H2_NF_5	H3_NF_1	H3_NF_2	H3_NF_3	H3_NF_4	H3_NF_5	H1_EF_1	H2_EF_1	H2_EF_2	H2_EF_3	H2_EF_4	H2_EF_5	H3_EF_1	H3_EF_2	H3_EF_3	H3_EF_4	H3_EF_5	H1_D0_1_diluted	H1_D0_2_diluted	H1_D0_3_diluted	H1_D0_4_diluted	H1_D0_5_diluted	H2_D0_1_diluted	H2_D0_2_diluted	H2_D0_3_diluted	H2_D0_4_diluted	H2_D0_5_diluted	H3_D0_1_diluted	H3_D0_2_diluted	H3_D0_3_diluted	H3_D0_4_diluted	H3_D0_5_diluted	H1_D1_1_diluted	H1_D1_2_diluted	H1_D1_3_diluted	H1_D1_4_diluted	H1_D1_5_diluted	H2_D1_1_diluted	H2_D1_2_diluted	H2_D1_3_diluted	H2_D1_4_diluted	H2_D1_5_diluted	H3_D1_1_diluted	H3_D1_2_diluted	H3_D1_3_diluted	H3_D1_4_diluted	H3_D1_5_diluted	H1_D3_1_diluted	H1_D3_2_diluted	H1_D3_3_diluted	H1_D3_4_diluted	H1_D3_5_diluted	H2_D3_1_diluted	H2_D3_2_diluted	H2_D3_3_diluted	H2_D3_4_diluted	H2_D3_5_diluted	H3_D3_1_diluted	H3_D3_2_diluted	H3_D3_3_diluted	H3_D3_4_diluted	H3_D3_5_diluted	H1_D5_1_diluted	H1_D5_2_diluted	H1_D5_3_diluted	H1_D5_4_diluted	H1_D5_5_diluted	H2_D5_1_diluted	H2_D5_2_diluted	H2_D5_3_diluted	H2_D5_4_diluted	H2_D5_5_diluted	H3_D5_1_diluted	H3_D5_2_diluted	H3_D5_3_diluted	H3_D5_4_diluted	H3_D5_5_diluted	H1_D7_1_diluted	H1_D7_2_diluted	H1_D7_3_diluted	H1_D7_4_diluted	H1_D7_5_diluted	H2_D7_1_diluted	H2_D7_2_diluted	H2_D7_3_diluted	H2_D7_4_diluted	H2_D7_5_diluted	H3_D7_1_diluted	H3_D7_2_diluted	H3_D7_3_diluted	H3_D7_4_diluted	H3_D7_5_diluted	H1_NF_1_diluted	H2_NF_1_diluted	H2_NF_2_diluted	H2_NF_3_diluted	H2_NF_4_diluted	H2_NF_5_diluted	H3_NF_1_diluted	H3_NF_2_diluted	H3_NF_3_diluted	H3_NF_4_diluted	H3_NF_5_diluted	H1_EF_1_diluted	H2_EF_1_diluted	H2_EF_2_diluted	H2_EF_3_diluted	H2_EF_4_diluted	H2_EF_5_diluted	H3_EF_1_diluted	H3_EF_2_diluted	H3_EF_3_diluted	H3_EF_4_diluted	H3_EF_5_diluted
Factors	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:newly_emerged | age of workers in days:0 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:1 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:3 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:establishment | age of workers in days:5 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:nurse | age of workers in days:7-10 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:new_foragers | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut	life stage:experienced_forager | age of workers in days:>12 | Sample source:gut
Propionate	1.355	1.665	1.235	1.515	1.955	1.415	1.405	1.37	1.465	1.48	1.885	2.395	2.04	2.215	2.355	2.84	2.735	3.315	3.02	2.72	2.375	1.71	3.13	2.965	3.07	4.305	1.62	2.995	2.755	4.545	14.95	58.5	59.5	29.4	18	44.2	41.85	62	61	61	7.45	28.85	9.15	33.3	29.85	40.55	40	52.5	71.5	34.55	74	70	79.5	55	54	102.5	93	51.5	143	88.5	88.5	166.5	107.5	96.5	45.9	82.5	85.5	93	25.65	67.5	75	94	116	78.5	84	93.5	61	17.65	43.6	17.55	22.8	72.5	55.5	19.65	17.55	12.9	9.85	22.3	56	32.05	2.48	9.4	9.15	11.35	14.35	1.59	14.2																																																																																																	
Acetate																																																																																																		16	97.5	13.4	12.75	21.95	18.3	10.1	10.55	10.6	9.8	66.5	46	13.9	20.85	22.05	109.25	322.5	362.5	427.5	492.5	647.5	68.25	520	287.5	690	702.5	183.5	615	522.5	475	900	1250	1542.5	1192.5	1545	1357.5	1317.5	1730	1477.5	1192.5	1240	1417.5	1067.5	1275	1155	967.5	1465	1810	1447.5	812.5	1762.5	1930	2102.5	1857.5	1160	2047.5	2015	1972.5	2092.5	1575	1447.5	1847.5	1787.5	1640	1327.5	1262.5	1345	1147.5	632.5	1322.5	1485	1695	1460	1367.5	1587.5	2220	1170	800	347.5	590	627.5	1035	1112.5	595	457.5	552.5	787.5	580	1485	617.5	159	367.5	700	512.5	557.5	98	485
Butyrate	16	97.5	13.4	12.75	21.95	18.3	10.1	10.55	10.6	9.8	66.5	46	13.9	20.85	22.05	109.25	322.5	362.5	427.5	492.5	647.5	68.25	520	287.5	690	702.5	183.5	615	522.5	475	900	1250	1542.5	1192.5	1545	1357.5	1317.5	1730	1477.5	1192.5	1240	1417.5	1067.5	1275	1155	967.5	1465	1810	1447.5	812.5	1762.5	1930	2102.5	1857.5	1160	2047.5	2015	1972.5	2092.5	1575	1447.5	1847.5	1787.5	1640	1327.5	1262.5	1345	1147.5	632.5	1322.5	1485	1695	1460	1367.5	1587.5	2220	1170	800	347.5	590	627.5	1035	1112.5	595	457.5	552.5	787.5	580	1485	617.5	159	367.5	700	512.5	557.5	98	485																																																																																																	
Valerate	1.13	1.385	1.085	1.165	1.285	1.41	1.12	1.125	1.325	1.16	1.825	1.715	1.53	1.51	1.63	1.975	2.275	1.985	3.005	3.025	3.025	1.32	2.26	2.44	2.445	2.65	1.76	2.07	2.21	2.42	8	6.3	13.3	8.2	8.3	4.765	4.94	11.5	6.05	10.85	4.975	8.25	3.065	6.8	11.85	4.825	4.175	10.15	7.75	6.3	11.85	7.5	10.3	7.95	6.35	9.4	10.3	11.15	19.25	6.95	9.5	15.45	11.75	9.6	7.9	8.25	10.95	12	3.19	6.95	11.7	11.2	9.45	10.75	15.9	26.15	6.85	2.775	5.8	2.4	5.8	8.65	7.65	3.62	4.085	4.595	4.385	4.48	5.45	4.605	1.465	3.615	3.235	2.65	5.3	1.945	7.3																																																																																																	
Isovalerate	0.94	0.915	0.905	0.89	0.92	0.91	0.925	0.935	0.94	0.915	0.925	0.975	0.985	1.015	0.945	0.935	0.965	0.945	0.995	1.135	1.05	0.89	0.935	1.08	0.915	1.12	0.935	0.97	0.98	1.05	1.295	1.495	1.63	1.375	1.32	1.14	1.415	1.48	1.275	1.625	2.63	2.065	1.535	1.905	1.67	1.34	1.16	1.345	0.97	0.795	1.185	1.12	1.255	1.195	1.38	2.6	2.865	2.87	3.14	3.075	0.765	1.43	1.58	1.46	1.01	1.725	1.795	1.735	1.375	2.085	1.965	2.045	1.67	1.59	2.115	1.585	1.585	1.56	1.82	1.795	1.775	1.535	1.47	1.38	1.085	1.445	2.855	1.94	2.005	2.58	1.82	1.63	1.325	1.255	1.365	1.675	1.18																																																																																																	
Isobutyrate																0.313	0.262	0.424	0.955	0.64	0.835	0.062	0.436	0.655	0.302	0.208	0.098	0.202	0.131	0.76	4.775	19.35	6.7	3.215	9.3	5.25	4.47	5.9	8.05	6.6	3.75	1.94	0.7	4.38	2.19	7.25	6.05	6.1	17.6	15.2	14.3	14.6	20.15	11.8	11	14.9	13.4	20.8	16.4	10.4	12.4	14	18.85	10.35	13.9	9.75	10.95	11.9	3.985	16.45	7.2	12.15	8.35	10.55	13.35	25.35	9.05	4.855	10.6	2.795	2.97	7.3	8.9	5.3	4.075	7.35	2.38	2.475	9.5	3.56	0.83	1.88	4.43	4.07	6.45	1.58	5.35																																																																																																	
Lactate																																																																																																		10.5	91	7.45	6.3	8.8	5.05	8	5	5.6	5.55	37.3	25.4	6.85	7.25	6.65	290	415	812.5	975	637.5	312.5	118	385	355	765	105.25	257.5	385	224.5	485	21.75	362.5	60	68	59	36	179.5	91.25	44.25	79.25	212.25	229.5	462.5	166	186.25	14.05	373	14.3	34.15	10.95	23.85	15.9	15.75	19.45	80.5	52	34.05	150	21.3	400	97.25	47.5	16.55	39.65	20.55	82	31.3	18.5	32.1	31.9	41.1	26.3	30.15	17.5	25.25	32.25	18.7	37	36.5	157	523	10.75	17.9	175	20.9	43.55	2182.5	1105	48.85	194.25	227.25	395	142.25	99.5	198.5	2.335	108
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	m/z	KEGG	Pubchem SID	Pubchem CID
Acetate	194	C00033  	3335	176
Propionate	208	C00163	3463	1032
Butyrate	222	C00246	3545	264
Isobutyrate	222	C02632	5612	6590
Valerate	236	C00803	4061	7991
Isovalerate	236	C08262	10461	10430
Lactate	224	C00186  	3486	107689
METABOLITES_END
#END