#METABOLOMICS WORKBENCH engelmab_20241028_083820 DATATRACK_ID:5321 STUDY_ID:ST003554 ANALYSIS_ID:AN005843 PROJECT_ID:PR002188
VERSION             	1
CREATED_ON             	November 6, 2024, 8:16 pm
#PROJECT
PR:PROJECT_TITLE                 	Comprehension of the age-dependent gut and brain interaction of honeybee workers
PR:PROJECT_TITLE                 	by integration of multi omics approaches
PR:PROJECT_SUMMARY               	In honeybees, division of labour is a key feature, with age-related behavioural
PR:PROJECT_SUMMARY               	transitions being closely associated with molecular changes in the brain, gut,
PR:PROJECT_SUMMARY               	and microbiota. In this study, to investigate these molecular changes and thus
PR:PROJECT_SUMMARY               	better understand their contribution to behavioural responses and modulation, we
PR:PROJECT_SUMMARY               	analysed the global metabolomic shifts in honeybee workers and their microbiota
PR:PROJECT_SUMMARY               	throughout their lives. Overall, our findings provide new insights toward
PR:PROJECT_SUMMARY               	developing potential biomarkers for evaluation of different functional changes
PR:PROJECT_SUMMARY               	related to various environmental stressors.
PR:INSTITUTE                     	Helmholtz Centre for Environmental Research
PR:DEPARTMENT                    	Molecular Systems Biology
PR:LAST_NAME                     	Engelmann
PR:FIRST_NAME                    	Beatrice
PR:ADDRESS                       	Permoserstraße 15, Leipzipg, Saxony, 03418, Germany
PR:EMAIL                         	beatrice.engelmann@ufz.de
PR:PHONE                         	004934160251099
#STUDY
ST:STUDY_TITLE                   	Investigation of age-dependent changes in the brain of honeybee workers using
ST:STUDY_TITLE                   	targeted metabolomics for amino acid and biogenic amine determination
ST:STUDY_SUMMARY                 	In the honeybee guts we measured the concentrations of 21 amino acids and 6
ST:STUDY_SUMMARY                 	biogenic amines. Using a k-means clustering, the metabolites were grouped by
ST:STUDY_SUMMARY                 	their trends into six clusters. Tyramine and tyrosine are involved in the
ST:STUDY_SUMMARY                 	octopamine and dopamine biosynthesis. We found that all four metabolites were
ST:STUDY_SUMMARY                 	part of the same cluster, with their concentrations being significantly
ST:STUDY_SUMMARY                 	decreased in comparison to newly emerged workers from day 1 onwards for the
ST:STUDY_SUMMARY                 	entire duration of the study.
ST:INSTITUTE                     	Helmholtz Centre for Environmental Research
ST:DEPARTMENT                    	Molecular Systems Biology
ST:LAST_NAME                     	Engelmann
ST:FIRST_NAME                    	Beatrice
ST:ADDRESS                       	Permoserstraße 15, Leipzipg, Saxony, 03418, Germany
ST:EMAIL                         	beatrice.engelmann@ufz.de
ST:PHONE                         	004934160251099
#SUBJECT
SU:SUBJECT_TYPE                  	Insect
SU:SUBJECT_SPECIES               	Apis mellifera
SU:TAXONOMY_ID                   	7460
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	H1_D0_1	H1_D0_1	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_2	H1_D0_2	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_3	H1_D0_3	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_4	H1_D0_4	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_5	H1_D0_5	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_1	H1_D1_1	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_2	H1_D1_2	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_3	H1_D1_3	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_4	H1_D1_4	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_5	H1_D1_5	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_1	H1_D3_1	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_2	H1_D3_2	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_3	H1_D3_3	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_4	H1_D3_4	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_5	H1_D3_5	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_1	H1_D5_1	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_2	H1_D5_2	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_3	H1_D5_3	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_4	H1_D5_4	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_5	H1_D5_5	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_1	H1_D7_1	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_2	H1_D7_2	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_3	H1_D7_3	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_4	H1_D7_4	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_5	H1_D7_5	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_NF_1	H1_NF_1	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_EF_1	H1_EF_1	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_1	H1_D0_1_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_2	H1_D0_2_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_3	H1_D0_3_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_4	H1_D0_4_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D0_5	H1_D0_5_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_1	H1_D1_1_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_2	H1_D1_2_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_3	H1_D1_3_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_4	H1_D1_4_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D1_5	H1_D1_5_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_1	H1_D3_1_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_2	H1_D3_2_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_3	H1_D3_3_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_4	H1_D3_4_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D3_5	H1_D3_5_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_1	H1_D5_1_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_2	H1_D5_2_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_3	H1_D5_3_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_4	H1_D5_4_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D5_5	H1_D5_5_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_1	H1_D7_1_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_2	H1_D7_2_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_3	H1_D7_3_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_4	H1_D7_4_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_D7_5	H1_D7_5_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_NF_1	H1_NF_1_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H1_EF_1	H1_EF_1_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=1; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_1	H2_D0_1	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_2	H2_D0_2	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_3	H2_D0_3	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_4	H2_D0_4	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_5	H2_D0_5	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_1	H2_D1_1	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_2	H2_D1_2	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_3	H2_D1_3	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_4	H2_D1_4	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_5	H2_D1_5	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_1	H2_D3_1	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_2	H2_D3_2	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_3	H2_D3_3	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_4	H2_D3_4	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_5	H2_D3_5	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_1	H2_D5_1	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_2	H2_D5_2	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_3	H2_D5_3	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_4	H2_D5_4	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_5	H2_D5_5	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_1	H2_D7_1	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_2	H2_D7_2	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_3	H2_D7_3	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_4	H2_D7_4	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_5	H2_D7_5	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_1	H2_NF_1	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_2	H2_NF_2	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_3	H2_NF_3	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_4	H2_NF_4	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_5	H2_NF_5	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_1	H2_EF_1	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_2	H2_EF_2	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_3	H2_EF_3	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_4	H2_EF_4	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_5	H2_EF_5	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_1	H2_D0_1_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_2	H2_D0_2_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_3	H2_D0_3_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_4	H2_D0_4_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D0_5	H2_D0_5_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_1	H2_D1_1_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_2	H2_D1_2_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_3	H2_D1_3_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_4	H2_D1_4_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D1_5	H2_D1_5_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_1	H2_D3_1_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_2	H2_D3_2_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_3	H2_D3_3_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_4	H2_D3_4_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D3_5	H2_D3_5_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_1	H2_D5_1_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_2	H2_D5_2_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_3	H2_D5_3_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_4	H2_D5_4_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D5_5	H2_D5_5_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_1	H2_D7_1_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_2	H2_D7_2_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_3	H2_D7_3_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_4	H2_D7_4_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_D7_5	H2_D7_5_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_1	H2_NF_1_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_2	H2_NF_2_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_3	H2_NF_3_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_4	H2_NF_4_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_NF_5	H2_NF_5_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_1	H2_EF_1_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_2	H2_EF_2_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_3	H2_EF_3_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_4	H2_EF_4_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H2_EF_5	H2_EF_5_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=2; RAW_FILE_NAME(Raw_data)=240405_CU_Eli_Batch2_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_1	H3_D0_1	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_2	H3_D0_2	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_3	H3_D0_3	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_4	H3_D0_4	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_5	H3_D0_5	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_1	H3_D1_1	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_2	H3_D1_2	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_3	H3_D1_3	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_4	H3_D1_4	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_5	H3_D1_5	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_1	H3_D3_1	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_2	H3_D3_2	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_3	H3_D3_3	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_4	H3_D3_4	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_5	H3_D3_5	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_1	H3_D5_1	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_2	H3_D5_2	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_3	H3_D5_3	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_4	H3_D5_4	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_5	H3_D5_5	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_1	H3_D7_1	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_2	H3_D7_2	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_3	H3_D7_3	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_4	H3_D7_4	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_5	H3_D7_5	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_1	H3_NF_1	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_2	H3_NF_2	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_3	H3_NF_3	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_4	H3_NF_4	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_5	H3_NF_5	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_1	H3_EF_1	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_2	H3_EF_2	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_3	H3_EF_3	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_4	H3_EF_4	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_5	H3_EF_5	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240320_Eli_YY_Bee_Brain_undiluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_1	H3_D0_1_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_2	H3_D0_2_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_3	H3_D0_3_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_4	H3_D0_4_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D0_5	H3_D0_5_diluted	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_1	H3_D1_1_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_2	H3_D1_2_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_3	H3_D1_3_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_4	H3_D1_4_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D1_5	H3_D1_5_diluted	life stage:establishment | age of workers in days:1 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_1	H3_D3_1_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_2	H3_D3_2_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_3	H3_D3_3_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_4	H3_D3_4_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D3_5	H3_D3_5_diluted	life stage:establishment | age of workers in days:3 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_1	H3_D5_1_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_2	H3_D5_2_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_3	H3_D5_3_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_4	H3_D5_4_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D5_5	H3_D5_5_diluted	life stage:establishment | age of workers in days:5 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_1	H3_D7_1_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_2	H3_D7_2_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_3	H3_D7_3_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_4	H3_D7_4_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_D7_5	H3_D7_5_diluted	life stage:nurse | age of workers in days:7-10 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_1	H3_NF_1_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_2	H3_NF_2_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_3	H3_NF_3_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_4	H3_NF_4_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_NF_5	H3_NF_5_diluted	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_1	H3_EF_1_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_2	H3_EF_2_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_3	H3_EF_3_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_4	H3_EF_4_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
SUBJECT_SAMPLE_FACTORS           	H3_EF_5	H3_EF_5_diluted	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	hive=3; RAW_FILE_NAME(Raw_data)=240404_CU_Eli_Batch1_Brain_diluted.wiff
#COLLECTION
CO:COLLECTION_SUMMARY            	Workers were pooled to obtain a final sample number of n = 5. The brain(s) and
CO:COLLECTION_SUMMARY            	gut(s) from the same workers were assigned the same sample names. In total, we
CO:COLLECTION_SUMMARY            	had 15 samples from each age group, with the exception of the foraging stages,
CO:COLLECTION_SUMMARY            	where owing to the lack of foragers from Hive 1, we only had 11. All sampled
CO:COLLECTION_SUMMARY            	bees were placed directly in liquid nitrogen to prevent changes in the metabolic
CO:COLLECTION_SUMMARY            	profile. They were then decapitated in the laboratory so that the head and body
CO:COLLECTION_SUMMARY            	of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for
CO:COLLECTION_SUMMARY            	later dissection. Honeybee brains were dissected using a dissecting microscope
CO:COLLECTION_SUMMARY            	(SZX7, Olympus). The head was placed on a petri dish lined with SYLGARD® 184
CO:COLLECTION_SUMMARY            	silicone. Before pinning the head for dissection, antennae and mouthpieces were
CO:COLLECTION_SUMMARY            	removed. Subsequently, double-distilled water (ddH2O) was added until the head
CO:COLLECTION_SUMMARY            	was fully submerged to facilitate dissection. The head cuticle was removed, and
CO:COLLECTION_SUMMARY            	the hypopharyngeal glands and trachea were extracted from the inside. The brain
CO:COLLECTION_SUMMARY            	was then separated from the cuticle and remaining tracheal remnants were removed
CO:COLLECTION_SUMMARY            	before transferring the clean brain to a new Eppendorf tube.
CO:SAMPLE_TYPE                   	Bee brain
#TREATMENT
TR:TREATMENT_SUMMARY             	On the day of newly emerged workers (day 0), bees were samples as a baseline for
TR:TREATMENT_SUMMARY             	no or low bacterial abundance in the gut. Moreover, bees from each hive were
TR:TREATMENT_SUMMARY             	collected on Day 1, 3 and 5 to cover the establishment phase of the gut. Samples
TR:TREATMENT_SUMMARY             	for the in-hive worker phase, defined as workers inside the hives that have a
TR:TREATMENT_SUMMARY             	fully developed microbiome, were taken every day from Day 7 to Day 10. For the
TR:TREATMENT_SUMMARY             	foraging phase, we differentiated between new and experienced foragers, as these
TR:TREATMENT_SUMMARY             	show different behaviour due to different levels of experience.Based on
TR:TREATMENT_SUMMARY             	experience, they were either collected one to two days after onset (new
TR:TREATMENT_SUMMARY             	foragers) or at least four days after onset (experienced foragers).
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	In brief, x mg pool brain was mixed with five times the volume (in µL) of
SP:SAMPLEPREP_SUMMARY            	acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30
SP:SAMPLEPREP_SUMMARY            	Hz, 10 min; Retsch Qiagen). After Centrifugation (2 min, 14000 rpm), 10 µL were
SP:SAMPLEPREP_SUMMARY            	used for amino acid derivatization. First, the supernatant was evaporated to
SP:SAMPLEPREP_SUMMARY            	dryness (SpeedVac, Eppendorf), resuspended in 50 µL of 5% phenyl isothiocyanate
SP:SAMPLEPREP_SUMMARY            	(PITC) in ethanol: H2O: pyridine (1:1:1, v/v/v), and incubated for 25 min at RT.
SP:SAMPLEPREP_SUMMARY            	Subsequently, the samples were dried to remove excess PITC and resuspended in 10
SP:SAMPLEPREP_SUMMARY            	µL 5 mM ammonium acetate in methanol. After incubation (10 min at 14,000 rpm)
SP:SAMPLEPREP_SUMMARY            	90 µL of H2O: ACN + 0.2% formic acid were added. Each derivative was measured
SP:SAMPLEPREP_SUMMARY            	in an undiluted and diluted (1:25) manner.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Waters Acquity
CH:COLUMN_NAME                   	Zorbax Eclipse XDB-C18 (100 x 3.0mm, 3.5um)
CH:SOLVENT_A                     	100% water; 0.2% formic acid
CH:SOLVENT_B                     	100% acetonitrile; 0.2% formic acid
CH:FLOW_GRADIENT                 	0-0.5 min at 0% B, 0.5-4 min 0-70% B, 4-5.3 min 70% B, 5.3-5.4 min 70-0% B,
CH:FLOW_GRADIENT                 	5.4-7.3 min 0% B
CH:FLOW_RATE                     	0.5 mL/min
CH:COLUMN_TEMPERATURE            	50
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 6500+ Qtrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	For identification and quantitation, a scheduled MRM method was used, with
MS:MS_COMMENTS                   	specific transitions for every amino acid and biogenic amine. Data acquisition
MS:MS_COMMENTS                   	and peak integration were performed in SciexOS software (Version 3.0.0.).
MS:MS_COMMENTS                   	Calculation of concentration was done using external calibration curves.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	µMol
MS_METABOLITE_DATA_START
Samples	H1_D0_1	H1_D0_2	H1_D0_3	H1_D0_4	H1_D0_5	H2_D0_1	H2_D0_2	H2_D0_3	H2_D0_4	H2_D0_5	H3_D0_1	H3_D0_2	H3_D0_3	H3_D0_4	H3_D0_5	H1_D1_1	H1_D1_2	H1_D1_3	H1_D1_4	H1_D1_5	H2_D1_1	H2_D1_2	H2_D1_3	H2_D1_4	H2_D1_5	H3_D1_1	H3_D1_2	H3_D1_3	H3_D1_4	H3_D1_5	H1_D3_1	H1_D3_2	H1_D3_3	H1_D3_4	H1_D3_5	H2_D3_1	H2_D3_2	H2_D3_3	H2_D3_4	H2_D3_5	H3_D3_1	H3_D3_2	H3_D3_3	H3_D3_4	H3_D3_5	H1_D5_1	H1_D5_2	H1_D5_3	H1_D5_4	H1_D5_5	H2_D5_1	H2_D5_2	H2_D5_3	H2_D5_4	H2_D5_5	H3_D5_1	H3_D5_2	H3_D5_3	H3_D5_4	H3_D5_5	H1_D7_1	H1_D7_2	H1_D7_3	H1_D7_4	H1_D7_5	H2_D7_1	H2_D7_2	H2_D7_3	H2_D7_4	H2_D7_5	H3_D7_1	H3_D7_2	H3_D7_3	H3_D7_4	H3_D7_5	H1_NF_1	H2_NF_1	H2_NF_2	H2_NF_3	H2_NF_4	H2_NF_5	H3_NF_1	H3_NF_2	H3_NF_3	H3_NF_4	H3_NF_5	H1_EF_1	H2_EF_1	H2_EF_2	H2_EF_3	H2_EF_4	H2_EF_5	H3_EF_1	H3_EF_2	H3_EF_3	H3_EF_4	H3_EF_5
Factors	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:newly_emerged | age of workers in days:0 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:1 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:3 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:establishment | age of workers in days:5 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:nurse | age of workers in days:7-10 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:new_foragers | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain	life stage:experienced_forager | age of workers in days:>12 | Sample source:brain
Tyramine	3.85E-02	3.68E-02	4.64E-02	3.51E-02	5.21E-02	3.26E-02	4.67E-02	7.88E-02	6.01E-02	6.81E-02	5.66E-02	3.19E-02	7.84E-02	1.01E-01	4.11E-02	2.18E-02	4.41E-02	3.41E-02	3.51E-02	5.05E-02	4.26E-02	2.34E-02	1.82E-02	2.82E-02	1.66E-02	2.49E-02	1.81E-02	2.22E-02	2.46E-02	1.75E-02	1.11E-02	1.17E-02	1.05E-02	1.11E-02	1.20E-02	1.10E-02	1.17E-02	1.22E-02	1.47E-02	1.20E-02	1.24E-02	1.11E-02	1.21E-02	1.23E-02	1.43E-02	1.15E-02	1.36E-02	1.24E-02	1.54E-02	1.24E-02	1.29E-02	1.19E-02	1.50E-02	1.23E-02	1.06E-02	1.30E-02	1.23E-02	1.22E-02	1.09E-02	1.17E-02	1.27E-02	1.33E-02	1.28E-02	1.22E-02	1.41E-02	1.25E-02	1.29E-02	1.19E-02	1.25E-02	1.35E-02	1.26E-02	1.25E-02	1.09E-02	1.11E-02	1.10E-02	1.45E-02	1.07E-02	1.15E-02	1.15E-02	1.38E-02	1.62E-02	1.22E-02	1.48E-02	1.08E-02	1.38E-02	1.47E-02	1.84E-02	1.60E-02	1.20E-02	1.46E-02	1.42E-02	1.42E-02	1.45E-02	1.45E-02	1.55E-02	1.29E-02	1.12E-02
Dopamine	5.52E-02	4.18E-02	7.68E-02	1.11E-01	9.92E-02	3.92E-02	6.33E-02	1.27E-01	4.78E-02	8.03E-02	4.27E-02	7.00E-02	1.36E-01	1.26E-01	5.68E-02	3.25E-02	1.26E-01	1.16E-01	8.54E-02	4.56E-02	4.20E-02	3.80E-02	2.96E-02	2.25E-02	3.90E-02	4.97E-02	7.58E-02	6.53E-02	6.90E-02	4.94E-02	2.23E-02	3.31E-02	3.35E-02	3.67E-02	3.23E-02	3.14E-02	2.88E-02	3.64E-02	4.24E-02	5.77E-02	2.19E-02	2.78E-02	4.62E-02	3.07E-02	7.18E-02	4.09E-02	3.78E-02	4.36E-02	6.26E-02	3.27E-02	3.46E-02	6.05E-02	9.96E-02	2.56E-02	3.66E-02	3.29E-02	3.62E-02	2.74E-02	3.51E-02	6.93E-02	4.08E-02	2.70E-02	3.56E-02	2.34E-02	4.74E-02	4.70E-02	3.65E-02	4.03E-02	4.27E-02	6.39E-02	2.52E-02	4.87E-02	2.44E-02	2.16E-02	2.91E-02	4.06E-02	2.83E-02	3.29E-02	5.70E-02	3.30E-02	3.10E-02	3.67E-02	3.65E-02	1.72E-02	2.35E-02	2.63E-02	2.71E-02	2.20E-02	2.04E-02	1.69E-02	2.01E-02	2.36E-02	2.46E-02	2.63E-02	2.68E-02	2.79E-02	1.81E-02
Kynurenine	2.21E-01	3.03E-01	2.37E-01	3.04E-01	1.88E-01	1.37E-01	2.08E-01	1.81E-01	2.50E-01	2.43E-01	1.56E-01	1.77E-01	1.68E-01	3.08E-01	2.87E-01	3.32E-01	2.81E-01	3.87E-01	2.95E-01	6.74E-01	1.95E-01	4.19E-01	1.73E-01	1.84E-01	8.97E-02	1.32E-01	2.54E-01	1.70E-01	2.09E-01	1.11E-01	2.00E-01	3.97E-01	1.37E-01	2.55E-01	1.62E-01	2.66E-01	3.28E-01	3.60E-01	2.65E-01	2.89E-01	2.90E-01	2.36E-01	2.91E-01	2.92E-01	2.57E-01	1.79E-01	1.33E-01	2.25E-01	1.49E-01	1.34E-01	1.78E-01	1.63E-01	3.63E-01	3.06E-01	2.55E-01	2.18E-01	2.27E-01	3.30E-01	2.62E-01	2.42E-01	1.70E-01	5.30E-02	8.94E-02	6.69E-02	6.67E-02	8.83E-02	1.23E-01	1.18E-01	1.02E-01	1.84E-01	1.75E-01	1.44E-01	1.90E-01	1.54E-01	8.65E-02	6.99E-02	3.18E-01	2.09E-01	2.96E-01	4.26E-02	2.06E-02	1.35E-01	1.26E-01	1.14E-01	6.23E-02	1.33E-01	7.31E-02	3.25E-02	7.30E-02	5.13E-02	4.24E-02	2.46E-02	3.34E-02	2.74E-02	4.15E-02	1.38E-01	5.33E-02
Citrulline	5.89E+00	3.52E+00	2.83E+00	3.08E+00	2.74E+00	1.55E+00	3.15E+00	2.38E+00	2.63E+00	2.29E+00	2.29E+00	2.99E+00	2.68E+00	2.67E+00	3.05E+00	2.22E+00	2.04E+00	2.52E+00	2.38E+00	3.53E+00	1.95E+00	3.03E+00	1.95E+00	2.57E+00	2.35E+00	2.69E+00	3.59E+00	2.52E+00	3.05E+00	2.82E+00	1.87E+00	2.41E+00	1.77E+00	1.96E+00	2.75E+00	3.09E+00	2.53E+00	1.99E+00	2.14E+00	2.24E+00	1.76E+00	2.08E+00	2.26E+00	2.34E+00	2.44E+00	3.34E+00	2.77E+00	2.70E+00	2.05E+00	3.19E+00	1.98E+00	2.34E+00	2.48E+00	2.09E+00	2.01E+00	2.91E+00	1.94E+00	2.25E+00	1.91E+00	1.80E+00	6.75E+00	2.19E+00	1.74E+00	1.77E+00	2.18E+00	1.71E+00	1.99E+00	1.58E+00	1.69E+00	1.32E+00	2.16E+00	1.99E+00	1.76E+00	1.51E+00	1.70E+00	2.02E+00	1.33E+00	2.09E+00	1.61E+00	2.08E+00	1.50E+00	2.17E+00	2.93E+00	2.26E+00	3.08E+00	2.41E+00	3.16E+00	3.81E+00	2.73E+00	2.49E+00	2.90E+00	2.85E+00	2.52E+00	2.31E+00	2.68E+00	2.09E+00	4.93E+00
Serotonin	9.46E-02	8.70E-02	9.01E-02	1.11E-01	1.16E-01	3.84E-02	9.65E-02	9.84E-02	9.38E-02	1.07E-01	9.61E-02	9.54E-02	1.09E-01	1.10E-01	8.12E-02	6.60E-02	1.21E-01	1.09E-01	9.25E-02	9.64E-02	7.58E-02	6.41E-02	9.08E-02	7.39E-02	8.71E-02	6.51E-02	8.15E-02	8.85E-02	8.28E-02	1.06E-01	2.96E-02	3.15E-02	3.70E-02	3.56E-02	4.87E-02	4.45E-02	4.55E-02	6.99E-02	8.60E-02	7.90E-02	5.75E-02	5.21E-02	7.21E-02	5.43E-02	9.27E-02	8.56E-02	8.15E-02	7.78E-02	1.09E-01	7.11E-02	1.16E-01	9.59E-02	1.19E-01	7.27E-02	6.90E-02	8.49E-02	8.38E-02	5.52E-02	8.14E-02	9.77E-02	9.49E-02	8.70E-02	8.81E-02	8.22E-02	1.01E-01	1.13E-01	1.13E-01	1.14E-01	1.07E-01	1.21E-01	9.22E-02	1.02E-01	7.57E-02	8.65E-02	8.93E-02	9.47E-02	6.84E-02	7.93E-02	1.07E-01	9.70E-02	8.25E-02	8.07E-02	8.23E-02	5.15E-02	9.05E-02	6.63E-02	4.07E-02	7.52E-02	4.86E-02	5.72E-02	6.20E-02	5.80E-02	5.23E-02	6.86E-02	6.92E-02	6.52E-02	5.76E-02
Octopamine	2.73E-02	3.78E-02	3.81E-02	4.72E-02	5.45E-02	3.04E-02	4.99E-02	5.64E-02	5.34E-02	6.76E-02	5.42E-02	3.87E-02	4.96E-02	7.18E-02	4.14E-02	3.50E-02	4.85E-02	3.91E-02	3.66E-02	2.84E-02	3.26E-02	2.42E-02	2.60E-02	2.78E-02	2.50E-02	3.19E-02	3.64E-02	2.99E-02	3.79E-02	3.00E-02	2.78E-02	2.59E-02	2.19E-02	2.85E-02	2.21E-02	2.29E-02	2.28E-02	1.79E-02	2.14E-02	2.09E-02	2.43E-02	1.90E-02	2.55E-02	2.98E-02	3.67E-02	3.25E-02	3.70E-02	3.27E-02	8.32E-02	3.04E-02	2.87E-02	2.71E-02	2.67E-02	2.48E-02	2.64E-02	2.30E-02	2.56E-02	2.06E-02	2.01E-02	2.54E-02	2.50E-02	3.07E-02	2.95E-02	4.06E-02	3.13E-02	2.28E-02	3.36E-02	2.84E-02	3.06E-02	3.57E-02	2.04E-02	2.42E-02	2.33E-02	2.02E-02	2.20E-02	3.35E-02	3.62E-02	2.90E-02	3.65E-02	2.74E-02	2.55E-02	2.20E-02	2.75E-02	2.10E-02	2.64E-02	2.03E-02	3.08E-02	2.24E-02	2.13E-02	2.22E-02	2.77E-02	2.41E-02	1.88E-02	2.23E-02	2.16E-02	2.55E-02	3.35E-02
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	m/z	KEGG	Pubchem SID	Pubchem CID
Octopamine	289.95	C04227   	6898	440266
Citrulline	311.2	C00327 	3621	9750
Dopamine	289.2	C03758 	6517	681
Kynurenine	344.2	C00328 	3622	161166
Tyramine	273.21	C00483 	3766	5610
Serotonin	312.2	C00780	4041	5202
METABOLITES_END
#END