#METABOLOMICS WORKBENCH Sethjparker_20241101_174808 DATATRACK_ID:5335 STUDY_ID:ST003557 ANALYSIS_ID:AN005847 PROJECT_ID:PR002191 VERSION 1 CREATED_ON November 4, 2024, 12:56 pm #PROJECT PR:PROJECT_TITLE N-methyl-arginine is taken up into the liver, brain, and kidney of mice. PR:PROJECT_TYPE Manuscript PR:PROJECT_SUMMARY N-methyl-arginine was identified as a transported substrate through multiple PR:PROJECT_SUMMARY cationic amino acid transporters, which facilitate the uptake of lysine, PR:PROJECT_SUMMARY arginine, and other structurally related amino acids. In cell culture, PR:PROJECT_SUMMARY N-methyl-arginine inhibits the uptake and metabolism of lysine. In this project, PR:PROJECT_SUMMARY we aimed to determine if N-methyl-arginine administered to mice at 80 mg/kg was PR:PROJECT_SUMMARY sufficient to inhibit tissue lysine uptake and metabolism. Following a single PR:PROJECT_SUMMARY injection, N-methyl-arginine significantly accumulated in mouse plasma and PR:PROJECT_SUMMARY tissues but failed to significantly inhibit the level of lysine metabolites PR:PROJECT_SUMMARY (e.g., saccharopine). These results are in-line with in vitro results suggesting PR:PROJECT_SUMMARY that N-methyl-arginine is a relatively low affinity transport inhibitor. PR:INSTITUTE University of British Columbia PR:DEPARTMENT Biochemistry & Molecular Biology PR:LABORATORY Parker laboratory PR:LAST_NAME Parker PR:FIRST_NAME Seth PR:ADDRESS 950 W 28th Ave, Vancouver, British Columbia, V6H 0B3, Canada PR:EMAIL seth.parker@bcchr.ca PR:PHONE 6048753121 #STUDY ST:STUDY_TITLE Metabolomics analysis of kidney, brain, liver, and plasma from Aldh7a1-/- mice ST:STUDY_TITLE administered PBS or N-methyl-arginine (80 mg/kg) via a single intraperitoneal ST:STUDY_TITLE injection. ST:STUDY_SUMMARY Metabolite analysis of kidney, brain, liver, and plasma isolated from Aldh7a1-/- ST:STUDY_SUMMARY mice fed diets consisting of 0.9% w/w lysine and 18 ppm pyridoxine. Tissues were ST:STUDY_SUMMARY cryo-homogenized using a liquid nitrogen cooled mortar and pestle into a fine ST:STUDY_SUMMARY powder and approximately 25-50 mg of each tissue was transferred to a ST:STUDY_SUMMARY pre-weighed homogenization tube to which ~20 2.3 mm zirconia beads and 1 mL of ST:STUDY_SUMMARY 80% ice cold methanol were added. Tissues were homogenized using a bead mill and ST:STUDY_SUMMARY cleared by centrifugation at 4C, where each of the 3-4 cycles consisted of a 60 ST:STUDY_SUMMARY second homogenization at 6 m/s followed by a 30 second pause. A volume ST:STUDY_SUMMARY equivalent to 25 mg of tissue extract was transferred to a new tube, and 250 ul ST:STUDY_SUMMARY of 80% methanol containing 1 ul of stable isotope-labeled internal amino acid ST:STUDY_SUMMARY standard mix was added. Metabolites were concentrated using a SpeedVac until ST:STUDY_SUMMARY dry. For plasma metabolite analysis, 10 µl of plasma was added to 250 µl of ST:STUDY_SUMMARY 80% methanol containing 1 µl of stable isotope-labeled internal amino acid ST:STUDY_SUMMARY standard mix, vortexed at 4C for 10 minutes, and centrifuged. 0.9 ml of ST:STUDY_SUMMARY supernatant was transferred to a new tube and concentrated using a SpeedVac ST:STUDY_SUMMARY until dry. Metabolites were reconstituted into 25-50 µl of water, vortexed, ST:STUDY_SUMMARY centrifuged, and transferred to vials for analysis by LCMS. LCMS was performed ST:STUDY_SUMMARY using a ZIC-pHILIC LC column coupled to a Vanquish LC and a flow gradient ST:STUDY_SUMMARY consisting of 10 mM ammonium carbonate in water and pure acetonitrile. The LC ST:STUDY_SUMMARY was coupled to an Exploris 240 mass spectrometer operated in a polarity ST:STUDY_SUMMARY switching data-dependent Top 5 mode. Full MS scan parameters for both positive ST:STUDY_SUMMARY and negative mode were set to 67-1000 m/z at a resolution of 120k and ddMS2 were ST:STUDY_SUMMARY collected at a resolution of 30k. ST:INSTITUTE University of British Columbia ST:DEPARTMENT Biochemistry & Molecular Biology ST:LABORATORY Parker laboratory ST:LAST_NAME Parker ST:FIRST_NAME Seth ST:ADDRESS 950 W 28th Ave, Vancouver, British Columbia, V6H 0B3, Canada ST:EMAIL seth.parker@bcchr.ca ST:PHONE 6048753121 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENDER Male and female SU:ANIMAL_FEED 0.9% w/w lysine, 18 ppm pyridoxine (standard diets) #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - 1 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Sex=M; Age (weeks)=8.4; RAW_FILE_NAME(Raw file name)=1.RAW SUBJECT_SAMPLE_FACTORS - 2 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Sex=M; Age (weeks)=8.4; RAW_FILE_NAME(Raw file name)=2.RAW SUBJECT_SAMPLE_FACTORS - 3 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Sex=F; Age (weeks)=15.3; RAW_FILE_NAME(Raw file name)=3.RAW SUBJECT_SAMPLE_FACTORS - 4 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Sex=F; Age (weeks)=15.3; RAW_FILE_NAME(Raw file name)=4.RAW SUBJECT_SAMPLE_FACTORS - 5 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Sex=M; Age (weeks)=21.4; RAW_FILE_NAME(Raw file name)=5.RAW SUBJECT_SAMPLE_FACTORS - 6 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Sex=M; Age (weeks)=21.4; RAW_FILE_NAME(Raw file name)=6.RAW SUBJECT_SAMPLE_FACTORS - 7 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Sex=M; Age (weeks)=32.9; RAW_FILE_NAME(Raw file name)=7.RAW SUBJECT_SAMPLE_FACTORS - 8 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Sex=M; Age (weeks)=32.9; RAW_FILE_NAME(Raw file name)=8.RAW SUBJECT_SAMPLE_FACTORS - 9 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Sex=F; Age (weeks)=5.9; RAW_FILE_NAME(Raw file name)=9.RAW SUBJECT_SAMPLE_FACTORS - 10 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Sex=F; Age (weeks)=5.9; RAW_FILE_NAME(Raw file name)=10.RAW SUBJECT_SAMPLE_FACTORS - 11 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Sex=M; Age (weeks)=8.4; RAW_FILE_NAME(Raw file name)=11.RAW SUBJECT_SAMPLE_FACTORS - 12 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Sex=M; Age (weeks)=8.4; RAW_FILE_NAME(Raw file name)=12.RAW SUBJECT_SAMPLE_FACTORS - 13 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Sex=F; Age (weeks)=15.3; RAW_FILE_NAME(Raw file name)=13.RAW SUBJECT_SAMPLE_FACTORS - 14 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Sex=F; Age (weeks)=15.3; RAW_FILE_NAME(Raw file name)=14.RAW SUBJECT_SAMPLE_FACTORS - 15 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Sex=M; Age (weeks)=21.4; RAW_FILE_NAME(Raw file name)=15.RAW SUBJECT_SAMPLE_FACTORS - 16 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Sex=M; Age (weeks)=21.4; RAW_FILE_NAME(Raw file name)=16.RAW SUBJECT_SAMPLE_FACTORS - 17 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Sex=M; Age (weeks)=32.9; RAW_FILE_NAME(Raw file name)=17.RAW SUBJECT_SAMPLE_FACTORS - 18 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Sex=M; Age (weeks)=32.9; RAW_FILE_NAME(Raw file name)=18.RAW SUBJECT_SAMPLE_FACTORS - 19 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Sex=F; Age (weeks)=5.9; RAW_FILE_NAME(Raw file name)=19.RAW SUBJECT_SAMPLE_FACTORS - 20 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Sex=F; Age (weeks)=5.9; RAW_FILE_NAME(Raw file name)=20.RAW SUBJECT_SAMPLE_FACTORS - 21 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Sex=M; Age (weeks)=8.4; RAW_FILE_NAME(Raw file name)=21.RAW SUBJECT_SAMPLE_FACTORS - 22 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Kidney Sex=M; Age (weeks)=8.4; RAW_FILE_NAME(Raw file name)=22.RAW SUBJECT_SAMPLE_FACTORS - 23 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Sex=F; Age (weeks)=15.3; RAW_FILE_NAME(Raw file name)=23.RAW SUBJECT_SAMPLE_FACTORS - 24 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Kidney Sex=F; Age (weeks)=15.3; RAW_FILE_NAME(Raw file name)=24.RAW SUBJECT_SAMPLE_FACTORS - 25 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Sex=M; Age (weeks)=21.4; RAW_FILE_NAME(Raw file name)=25.RAW SUBJECT_SAMPLE_FACTORS - 26 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Kidney Sex=M; Age (weeks)=21.4; RAW_FILE_NAME(Raw file name)=26.RAW SUBJECT_SAMPLE_FACTORS - 27 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Sex=M; Age (weeks)=32.9; RAW_FILE_NAME(Raw file name)=27.RAW SUBJECT_SAMPLE_FACTORS - 28 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Kidney Sex=M; Age (weeks)=32.9; RAW_FILE_NAME(Raw file name)=28.RAW SUBJECT_SAMPLE_FACTORS - 29 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Sex=F; Age (weeks)=5.9; RAW_FILE_NAME(Raw file name)=29.RAW SUBJECT_SAMPLE_FACTORS - 31 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Sex=M; Age (weeks)=8.4; RAW_FILE_NAME(Raw file name)=31.RAW SUBJECT_SAMPLE_FACTORS - 32 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma Sex=M; Age (weeks)=8.4; RAW_FILE_NAME(Raw file name)=32.RAW SUBJECT_SAMPLE_FACTORS - 33 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Sex=F; Age (weeks)=15.3; RAW_FILE_NAME(Raw file name)=33.RAW SUBJECT_SAMPLE_FACTORS - 34 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma Sex=F; Age (weeks)=15.3; RAW_FILE_NAME(Raw file name)=34.RAW SUBJECT_SAMPLE_FACTORS - 35 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Sex=M; Age (weeks)=21.4; RAW_FILE_NAME(Raw file name)=35.RAW SUBJECT_SAMPLE_FACTORS - 36 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma Sex=M; Age (weeks)=21.4; RAW_FILE_NAME(Raw file name)=36.RAW SUBJECT_SAMPLE_FACTORS - 37 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Sex=M; Age (weeks)=32.9; RAW_FILE_NAME(Raw file name)=37.RAW SUBJECT_SAMPLE_FACTORS - 38 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma Sex=M; Age (weeks)=32.9; RAW_FILE_NAME(Raw file name)=38.RAW SUBJECT_SAMPLE_FACTORS - 39 Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Sex=F; Age (weeks)=5.9; RAW_FILE_NAME(Raw file name)=39.RAW SUBJECT_SAMPLE_FACTORS - 40 Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma Sex=F; Age (weeks)=5.9; RAW_FILE_NAME(Raw file name)=40.RAW #COLLECTION CO:COLLECTION_SUMMARY Liver, brain, kidney, or plasma samples collected from mice treated with PBS or CO:COLLECTION_SUMMARY N-methyl-arginine (80 mg/kg) as a single intraperitoneal injection. Tissues CO:COLLECTION_SUMMARY collected 7-hours after injection. CO:SAMPLE_TYPE Tissue or Plasma #TREATMENT TR:TREATMENT_SUMMARY Mice were administered a single intraperitoneal injection containing either TR:TREATMENT_SUMMARY saline or N-methyl-arginine (80 mg/kg). 7-hours after the injection, the mice TR:TREATMENT_SUMMARY were euthanized by cardiac puncture, and tissues were isolated for analysis by TR:TREATMENT_SUMMARY LCMS. TR:TREATMENT_COMPOUND N-methyl-arginine TR:TREATMENT_ROUTE Intraperitoneal TR:TREATMENT_DOSE 80 mg/kg TR:TREATMENT_VEHICLE PBS #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Dried samples were reconstituted in 50 µL of HPLC-grade water. Samples were SP:SAMPLEPREP_SUMMARY vortexed for ~10 minutes, then centrifuged at 21,000 x g for 15 min at 4°C. 40 SP:SAMPLEPREP_SUMMARY microliters were transferred to LC vials containing glass inserts for analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um) CH:SOLVENT_A 100% water, 10 mM Ammonium Carbonate, pH 9.0 CH:SOLVENT_B 100% acetonitrile CH:FLOW_GRADIENT 80-20%B (0-30 min), 20-20%B (30-40 minute), and 20-80%B (40-40.5 minute); the LC CH:FLOW_GRADIENT column was re-equilibrated using 80-80%B from 40.5-52 minute before subsequent CH:FLOW_GRADIENT injections CH:FLOW_RATE 100 uL/min CH:COLUMN_TEMPERATURE 25 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Exploris 240 MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE POSITIVE MS:MS_COMMENTS The LC was coupled to a Thermo Scientific Exploris 240 mass spectrometer MS:MS_COMMENTS operating in heated electrospray ionization mode (HESI) for analysis. The MS:MS_COMMENTS following parameters were set for HESI: spray voltage 3.4 kV (positive) and 2 kV MS:MS_COMMENTS (negative), static spray voltage, sheath gas 25, aux gas 5, sweep gas 0.5, ion MS:MS_COMMENTS transfer tube temperature 320oC, and vaporizer temperature 75oC. The global MS:MS_COMMENTS parameters included an expected peak width of 20 seconds, mild trapping, and a MS:MS_COMMENTS default charge state of 1. A 40-min polarity switching data-dependent Top 5 MS:MS_COMMENTS method was used for positive mode and a data-dependent Top 3 method was used for MS:MS_COMMENTS negative mode. Full MS scan parameters for both positive and negative modes were MS:MS_COMMENTS set as follows: scan range 67-1000 m/z collected in profile mode, Orbitrap MS:MS_COMMENTS resolution 120,000, RF lens 70%, AGC target of 300%, and maximum injection time MS:MS_COMMENTS set to automatic. ddMS2 for positive mode were collected in centroid mode at an MS:MS_COMMENTS Orbitrap resolution of 30,000, isolation window of 1.5 m/z, an AGC target set to MS:MS_COMMENTS standard, a maximum injection time set to automatic, and a normalized collision MS:MS_COMMENTS energy set to 10%, 30%, and 80%. ddMS2 for negative mode were collected in MS:MS_COMMENTS centroid mode at an Orbitrap resolution of 30,000, isolation window of 2 m/z, an MS:MS_COMMENTS AGC target set to standard, a maximum injection time set to automatic, and a MS:MS_COMMENTS normalized collision energy set to 30%. For both positive and negative ddMS2, we MS:MS_COMMENTS applied an intensity threshold of 5e4 and a dynamic exclusion of 5 ppm for 10 MS:MS_COMMENTS seconds, excluding isotopes. A targeted selected ion monitoring (tSIM) scan was MS:MS_COMMENTS also included for pipecolate and P6C/P2C, and the retention time ranges were MS:MS_COMMENTS based on elution of authentic standards (pipecolate) or from positive samples MS:MS_COMMENTS (Aldh7a1-deficient tissues). The tSIM scan for pipecolate was collected from MS:MS_COMMENTS 8-12 minutes in negative mode at an isolation window of 4 m/z (for metabolomics) MS:MS_COMMENTS or 18 m/z (for isotope-tracing experiments, to include m/z shifts), an Orbitrap MS:MS_COMMENTS resolution of 120,000, a RF lens at 70%, an automatic maximum injection time, MS:MS_COMMENTS and collected in profile mode. The tSIM scan for P6C/P2C was collected from 6-10 MS:MS_COMMENTS minutes in positive mode at an isolation window of 4 m/z (for metabolomics) or MS:MS_COMMENTS 18 m/z (for isotope-tracing experiments, to include m/z shifts), an Orbitrap MS:MS_COMMENTS resolution of 120,000, a RF lens at 70%, an automatic maximum injection time, MS:MS_COMMENTS and collected in profile mode. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Ion counts MS_METABOLITE_DATA_START Samples 1 3 5 7 9 2 4 6 8 10 11 13 15 17 19 12 14 16 18 20 21 23 25 27 29 22 24 26 28 31 33 35 37 39 32 34 36 38 40 Factors Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Brain Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Liver Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Kidney Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Kidney Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Kidney Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Kidney Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Kidney Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Genotype:Aldh7a1-/- | Treatment:PBS | Sample source:Plasma Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma Genotype:Aldh7a1-/- | Treatment:N-methyl-arginine (80 mg/kg) | Sample source:Plasma LYSINE 7546210.27 2259226.905 6024695.419 7944601.07 9387464.877 6841485.916 5809087.472 694764.2985 7234934.124 7347104.09 13460609.69 10898705.72 7810508.255 6417777.88 19834829.81 10477405.22 15821591.19 18913657.11 12482848.4 6495606.079 13457375.41 13049268.9 8199074.389 11355735.6 5613912.612 15466547.8 12227643.36 2744713.702 8505054.952 7700105.181 2219457.644 3507230.763 2287961.064 99016.1827 3438476.982 102660.2022 4271052.756 1832563.356 3760624.191 SACCHAROPINE 925216.7201 952805.0365 1201715.897 1057473.894 1327349.514 1770386.555 784474.0558 542497.2663 781032.3051 1857121.866 2839519.681 1266117.429 1322223.303 654868.9517 2237486.319 3343912.207 4426710.243 5038074.3 2181537.841 3048595.825 2543169.974 2137491.014 2284319.338 2680219.524 1795280.565 3363221.744 1645679.221 3584547.492 2903134.373 98764.70034 19903.81588 21358.70493 13370.18181 30880.36224 25006.59916 18290.81814 21571.09852 19081.81633 22241.02637 PIPERIDEINE 6-CARBOXYLATE (P6C) 4376418.24 5316705.228 3291530.834 4433116.009 7286092.584 2408849.097 4607338.423 1541657.901 6433322.324 4563928.176 3607206.578 2425546.035 1389685.536 2160681.849 4633378.401 3825004.951 2832931.229 3896055.485 3855844.161 3873090.042 1630229.174 4488683.268 1762488.697 4003763.632 8502440.425 2708743.334 3432169.022 6072395.359 3935664.047 7306099.458 3837529.707 6670191.214 4589707.285 8814347.843 8417972.804 7883629.639 2941763.299 5180551.551 4274569.006 AMINOADIPATE 515977.3967 790312.1645 651313.2598 733737.8086 1070590.669 625812.4851 475280.5337 165790.118 733981.509 654939.0753 2093103.703 1680927.713 660961.7698 681368.6827 6245335.488 4043977.901 3051283.146 5338636.848 1546020.807 5147725.871 3558999.811 5629440.102 2483683.121 2436884.46 7781106.635 8856573.451 3624758.259 2346584.834 2989986.758 358955.5148 332062.1302 405685.1764 215369.5889 667832.9733 647797.8824 286331.6005 337886.5262 283378.7694 439503.6365 NG-METHYL-ARGININE 251827.5662 172270.7422 205892.8916 254848.5867 326780.6727 371231.0304 268826.5391 284185.7235 441177.6328 883400.9922 1371606.966 1145957.909 684140.1755 751846.2347 2074979.969 3444418.176 2086465.688 2411545.828 2890552.629 7084131.18 1331575.427 1439355.702 874599.6526 1551695.998 1482600.48 2731629.474 1715630.564 1483329.774 2206892.236 334270.5886 278164.8449 262560.9507 157854.7304 680615.0368 1113365.621 467282.3679 709699.0189 972886.3619 2684356.854 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Mode m/z Retention Time (min) HMDB ID LYSINE Positive 147.113353 26.05 HMDB0000182 SACCHAROPINE Positive 277.13901 13.74 HMDB0000279 PIPERIDEINE 6-CARBOXYLATE (P6C) Positive 128.07061 8.43 HMDB0012130 AMINOADIPATE Positive 162.0761 12.8 HMDB0000510 NG-METHYL-ARGININE Positive 189.1346 26.1 HMDB0000182 METABOLITES_END #END