#METABOLOMICS WORKBENCH boyuan_20240928_110822 DATATRACK_ID:5246 STUDY_ID:ST003596 ANALYSIS_ID:AN005906 PROJECT_ID:PR002224
VERSION             	1
CREATED_ON             	November 26, 2024, 5:16 pm
#PROJECT
PR:PROJECT_TITLE                 	An Organism-Level Quantitative Flux Model of Energy Metabolism in Mice
PR:PROJECT_SUMMARY               	Mammalian tissues feed on nutrients in the blood circulation. At the
PR:PROJECT_SUMMARY               	organism-level, mammalian energy metabolism comprises of oxidation, storage,
PR:PROJECT_SUMMARY               	interconverting, and releasing of circulating nutrients. Though much is known
PR:PROJECT_SUMMARY               	about the individual processes and nutrients, a holistic and quantitative model
PR:PROJECT_SUMMARY               	describing these processes for all major circulating nutrients is lacking. Here,
PR:PROJECT_SUMMARY               	by integrating isotope tracer infusion, mass spectrometry, and isotope gas
PR:PROJECT_SUMMARY               	analyzer measurement, we developed a framework to systematically quantify fluxes
PR:PROJECT_SUMMARY               	through these metabolic processes for 10 major circulating energy nutrients in
PR:PROJECT_SUMMARY               	mice, resulting in an organism-level quantitative flux model of energy
PR:PROJECT_SUMMARY               	metabolism. This model revealed in wildtype mice that circulating nutrients have
PR:PROJECT_SUMMARY               	more dominant metabolic cycling fluxes than their oxidation fluxes, with
PR:PROJECT_SUMMARY               	distinct partition between cycling and oxidation flux for individual circulating
PR:PROJECT_SUMMARY               	nutrients. Applications of this framework in obese mouse models showed on a per
PR:PROJECT_SUMMARY               	animal basis extensive elevation of metabolic cycling fluxes in ob/ob mice, but
PR:PROJECT_SUMMARY               	not in diet-induced obese mice. Thus, our framework describes quantitatively the
PR:PROJECT_SUMMARY               	functioning of energy metabolism at the organism-level, valuable for revealing
PR:PROJECT_SUMMARY               	new features of energy metabolism in physiological and disease conditions.
PR:INSTITUTE                     	Harvard School of Public Health
PR:DEPARTMENT                    	Molecular Metabolism
PR:LABORATORY                    	Sheng (Tony) Hui
PR:LAST_NAME                     	Yuan
PR:FIRST_NAME                    	Bo
PR:ADDRESS                       	655 Huntington Avenue
PR:EMAIL                         	bo_yuan@hsph.harvard.edu
PR:PHONE                         	7323189185
PR:FUNDING_SOURCE                	Paul G. Allen Family Foundation 0034665 (S.H.); NIH R00DK117066 (S.H.); NIH
PR:FUNDING_SOURCE                	R56DK140139 (S.H.); Sabri Ülker Center for Metabolic Research
#STUDY
ST:STUDY_TITLE                   	An Organism-Level Quantitative Flux Model of Energy Metabolism in Mice
ST:STUDY_SUMMARY                 	Mammalian tissues feed on nutrients in the blood circulation. At the
ST:STUDY_SUMMARY                 	organism-level, mammalian energy metabolism comprises of oxidation, storage,
ST:STUDY_SUMMARY                 	interconverting, and releasing of circulating nutrients. Though much is known
ST:STUDY_SUMMARY                 	about the individual processes and nutrients, a holistic and quantitative model
ST:STUDY_SUMMARY                 	describing these processes for all major circulating nutrients is lacking. Here,
ST:STUDY_SUMMARY                 	by integrating isotope tracer infusion, mass spectrometry, and isotope gas
ST:STUDY_SUMMARY                 	analyzer measurement, we developed a framework to systematically quantify fluxes
ST:STUDY_SUMMARY                 	through these metabolic processes for 10 major circulating energy nutrients in
ST:STUDY_SUMMARY                 	mice, resulting in an organism-level quantitative flux model of energy
ST:STUDY_SUMMARY                 	metabolism. This model revealed in wildtype C57BL/6J mice that circulating
ST:STUDY_SUMMARY                 	nutrients have more dominant metabolic cycling fluxes than their oxidation
ST:STUDY_SUMMARY                 	fluxes, with distinct partition between cycling and oxidation flux for
ST:STUDY_SUMMARY                 	individual circulating nutrients. Applications of this framework in obese mouse
ST:STUDY_SUMMARY                 	models showed on a per animal basis extensive elevation of metabolic cycling
ST:STUDY_SUMMARY                 	fluxes in ob/ob mice, but not in diet-induced obese C57BL/6J mice. Thus, our
ST:STUDY_SUMMARY                 	framework describes quantitatively the functioning of energy metabolism at the
ST:STUDY_SUMMARY                 	organism-level, valuable for revealing new features of energy metabolism in
ST:STUDY_SUMMARY                 	physiological and disease conditions.
ST:INSTITUTE                     	Harvard School of Public Health
ST:DEPARTMENT                    	Molecular Metabolism
ST:LABORATORY                    	Sheng (Tony) Hui
ST:LAST_NAME                     	Yuan
ST:FIRST_NAME                    	Bo
ST:ADDRESS                       	655 Huntington Avenue
ST:EMAIL                         	bo_yuan@hsph.harvard.edu
ST:PHONE                         	7323189185
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
SU:GENOTYPE_STRAIN               	C57BL/6J (control); high fat diet (HFD)-induced obese C57BL/6J mice; ob/ob.
SU:AGE_OR_AGE_RANGE              	12-24 weeks
SU:WEIGHT_OR_WEIGHT_RANGE        	25-35 g (control); 40-50 g (HFD); 50-65 (ob/ob)
SU:GENDER                        	Male
SU:ANIMAL_ANIMAL_SUPPLIER        	Jackson Laboratory
SU:ANIMAL_LIGHT_CYCLE            	7 AM to 7 PM
SU:ANIMAL_FEED                   	chow PicoLab Mouse Diet 5058; Research Diet D12492i, 60% calorie from fat
#FACTORS
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	L-art-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=L-art-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	L-art-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=5.52; RAW_FILE_NAME(Raw.file.name)=L-art-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	L-tail-L2	Genotype:wild_type | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3.132; RAW_FILE_NAME(Raw.file.name)=L-tail-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	L-tail-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=L-tail-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	L-tail-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=5.52; RAW_FILE_NAME(Raw.file.name)=L-tail-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	L-tail-O4	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=4.87; RAW_FILE_NAME(Raw.file.name)=L-tail-O4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	O-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=2.83; RAW_FILE_NAME(Raw.file.name)=O-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	O-art-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=O-art-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	O-art-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=4.59; RAW_FILE_NAME(Raw.file.name)=O-art-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	O-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=2.83; RAW_FILE_NAME(Raw.file.name)=O-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	O-tail-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=O-tail-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	O-tail-O5	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=4.74; RAW_FILE_NAME(Raw.file.name)=O-tail-O5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	O-tail-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=4.59; RAW_FILE_NAME(Raw.file.name)=O-tail-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	a-art-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.72; RAW_FILE_NAME(Raw.file.name)=a-art-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	aa-art-O8	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=3.872; RAW_FILE_NAME(Raw.file.name)=aa-art-O8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	aa-tail-L8	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.71; RAW_FILE_NAME(Raw.file.name)=aa-tail-L8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	aa-tail-O7	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=3.096; RAW_FILE_NAME(Raw.file.name)=aa-tail-O7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	aa-tail-O8	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=3.872; RAW_FILE_NAME(Raw.file.name)=aa-tail-O8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.63; RAW_FILE_NAME(Raw.file.name)=ab-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-art-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=4.432; RAW_FILE_NAME(Raw.file.name)=ab-art-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-art-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=4.464; RAW_FILE_NAME(Raw.file.name)=ab-art-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.63; RAW_FILE_NAME(Raw.file.name)=ab-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-tail-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=4.432; RAW_FILE_NAME(Raw.file.name)=ab-tail-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-tail-O5	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=4.432; RAW_FILE_NAME(Raw.file.name)=ab-tail-O5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-tail-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=4.464; RAW_FILE_NAME(Raw.file.name)=ab-tail-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ac-tail-L2	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=2.8; RAW_FILE_NAME(Raw.file.name)=ac-tail-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ac-tail-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=2.63; RAW_FILE_NAME(Raw.file.name)=ac-tail-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ac-tail-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=ac-tail-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ad-art-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.124; RAW_FILE_NAME(Raw.file.name)=ad-art-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ad-tail-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.124; RAW_FILE_NAME(Raw.file.name)=ad-tail-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ad-tail-O4	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.96; RAW_FILE_NAME(Raw.file.name)=ad-tail-O4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ad-tail-O8	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=447.002; infusion_uL_perMin=3.664; RAW_FILE_NAME(Raw.file.name)=ad-tail-O8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	af-O12-1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.3; infusion_uL_perMin=4.36; RAW_FILE_NAME(Raw.file.name)=af-O12-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	af-O13-1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.3; infusion_uL_perMin=4.604; RAW_FILE_NAME(Raw.file.name)=af-O13-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	af-O14-1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.3; infusion_uL_perMin=4.32; RAW_FILE_NAME(Raw.file.name)=af-O14-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ag-O10-1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.3; infusion_uL_perMin=4.328; RAW_FILE_NAME(Raw.file.name)=ag-O10-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ag-O11-1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.3; infusion_uL_perMin=4.576; RAW_FILE_NAME(Raw.file.name)=ag-O11-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ag-O15-1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.3; infusion_uL_perMin=4.368; RAW_FILE_NAME(Raw.file.name)=ag-O15-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ag-O16-1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.3; infusion_uL_perMin=4.016; RAW_FILE_NAME(Raw.file.name)=ag-O16-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ah-O17-1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=207.3; infusion_uL_perMin=4.76; RAW_FILE_NAME(Raw.file.name)=ah-O17-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ah-O19-1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=207.3; infusion_uL_perMin=3.816; RAW_FILE_NAME(Raw.file.name)=ah-O19-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	am-O31	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.3; infusion_uL_perMin=5.53; RAW_FILE_NAME(Raw.file.name)=am-O31.mzXML
SUBJECT_SAMPLE_FACTORS           	-	am-O33	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.3; infusion_uL_perMin=5.76; RAW_FILE_NAME(Raw.file.name)=am-O33.mzXML
SUBJECT_SAMPLE_FACTORS           	-	am-O34	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.3; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=am-O34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	an-O31	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=10.9; RAW_FILE_NAME(Raw.file.name)=an-O31.mzXML
SUBJECT_SAMPLE_FACTORS           	-	an-O33	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=11.46; RAW_FILE_NAME(Raw.file.name)=an-O33.mzXML
SUBJECT_SAMPLE_FACTORS           	-	an-O34	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=11.78; RAW_FILE_NAME(Raw.file.name)=an-O34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-L32	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=5.26; RAW_FILE_NAME(Raw.file.name)=ao-L32.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-L33	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=5.02; RAW_FILE_NAME(Raw.file.name)=ao-L33.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-L34	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=5.42; RAW_FILE_NAME(Raw.file.name)=ao-L34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-L35	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=5.46; RAW_FILE_NAME(Raw.file.name)=ao-L35.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-O31	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=11.04; RAW_FILE_NAME(Raw.file.name)=ao-O31.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-O32	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=12.06; RAW_FILE_NAME(Raw.file.name)=ao-O32.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-O34	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=11.82; RAW_FILE_NAME(Raw.file.name)=ao-O34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-O35	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=12.06; RAW_FILE_NAME(Raw.file.name)=ao-O35.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ap-L32	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=2.56; RAW_FILE_NAME(Raw.file.name)=ap-L32.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ap-O32	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=5.2; RAW_FILE_NAME(Raw.file.name)=ap-O32.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ap-O33	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=5.62; RAW_FILE_NAME(Raw.file.name)=ap-O33.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ap-O34	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=5.88; RAW_FILE_NAME(Raw.file.name)=ap-O34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ar-H1	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=4.45; RAW_FILE_NAME(Raw.file.name)=ar-H1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ar-L66	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.01; RAW_FILE_NAME(Raw.file.name)=ar-L66.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ar-O60	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=ar-O60.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ar-O61	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=ar-O61.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-H3	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=4.1; RAW_FILE_NAME(Raw.file.name)=as-H3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-H4	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.93; RAW_FILE_NAME(Raw.file.name)=as-H4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-L61	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.1; RAW_FILE_NAME(Raw.file.name)=as-L61.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-O53	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=as-O53.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-O54	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=as-O54.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-O58	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=as-O58.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-H1	Genotype:HFD | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=4.54; RAW_FILE_NAME(Raw.file.name)=at-H1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-H4	Genotype:HFD | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=3.94; RAW_FILE_NAME(Raw.file.name)=at-H4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-L62	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=3.31; RAW_FILE_NAME(Raw.file.name)=at-L62.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-L63	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=4.31; RAW_FILE_NAME(Raw.file.name)=at-L63.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-O51	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=at-O51.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-O56	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=at-O56.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H10	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.06; RAW_FILE_NAME(Raw.file.name)=az-H10.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H11	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.91; RAW_FILE_NAME(Raw.file.name)=az-H11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H7	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.48; RAW_FILE_NAME(Raw.file.name)=az-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H8	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.69; RAW_FILE_NAME(Raw.file.name)=az-H8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H9	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.68; RAW_FILE_NAME(Raw.file.name)=az-H9.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-L63	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.25; RAW_FILE_NAME(Raw.file.name)=az-L63.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-O58	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=az-O58.mzXML
SUBJECT_SAMPLE_FACTORS           	-	b-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.63; RAW_FILE_NAME(Raw.file.name)=b-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	b-art-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.34; RAW_FILE_NAME(Raw.file.name)=b-art-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	b-art-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=3.464; RAW_FILE_NAME(Raw.file.name)=b-art-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	b-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.63; RAW_FILE_NAME(Raw.file.name)=b-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-H1	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.5; RAW_FILE_NAME(Raw.file.name)=ba-H1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-H4	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.46; RAW_FILE_NAME(Raw.file.name)=ba-H4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-H5	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.5; RAW_FILE_NAME(Raw.file.name)=ba-H5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-H6	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.7; RAW_FILE_NAME(Raw.file.name)=ba-H6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-L66	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.1; RAW_FILE_NAME(Raw.file.name)=ba-L66.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-O61	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.5; RAW_FILE_NAME(Raw.file.name)=ba-O61.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H10	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.05; RAW_FILE_NAME(Raw.file.name)=bb-H10.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H11	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.91; RAW_FILE_NAME(Raw.file.name)=bb-H11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H13	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=3.94; RAW_FILE_NAME(Raw.file.name)=bb-H13.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H14	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.38; RAW_FILE_NAME(Raw.file.name)=bb-H14.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H7	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.46; RAW_FILE_NAME(Raw.file.name)=bb-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H8	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.68; RAW_FILE_NAME(Raw.file.name)=bb-H8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H9	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.58; RAW_FILE_NAME(Raw.file.name)=bb-H9.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bc-H15	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=8.92; RAW_FILE_NAME(Raw.file.name)=bc-H15.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bc-H17	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=9.9; RAW_FILE_NAME(Raw.file.name)=bc-H17.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bc-H18	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=8.02; RAW_FILE_NAME(Raw.file.name)=bc-H18.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bc-L62	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=6.46; RAW_FILE_NAME(Raw.file.name)=bc-L62.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bd-H10	Genotype:HFD | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4.18; RAW_FILE_NAME(Raw.file.name)=bd-H10.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bd-H7	Genotype:HFD | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4.72; RAW_FILE_NAME(Raw.file.name)=bd-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bd-L64	Genotype:wild_type | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=2.99; RAW_FILE_NAME(Raw.file.name)=bd-L64.mzXML
SUBJECT_SAMPLE_FACTORS           	-	be-H14	Genotype:HFD | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4.4; RAW_FILE_NAME(Raw.file.name)=be-H14.mzXML
SUBJECT_SAMPLE_FACTORS           	-	be-H22	Genotype:HFD | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4.38; RAW_FILE_NAME(Raw.file.name)=be-H22.mzXML
SUBJECT_SAMPLE_FACTORS           	-	be-L63	Genotype:wild_type | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4.23; RAW_FILE_NAME(Raw.file.name)=be-L63.mzXML
SUBJECT_SAMPLE_FACTORS           	-	be-O54	Genotype:ob_ob | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=5.6; RAW_FILE_NAME(Raw.file.name)=be-O54.mzXML
SUBJECT_SAMPLE_FACTORS           	-	be-O58	Genotype:ob_ob | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=5.6; RAW_FILE_NAME(Raw.file.name)=be-O58.mzXML
SUBJECT_SAMPLE_FACTORS           	-	be-O60	Genotype:ob_ob | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=5.6; RAW_FILE_NAME(Raw.file.name)=be-O60.mzXML
SUBJECT_SAMPLE_FACTORS           	-	be-O61	Genotype:ob_ob | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=5.6; RAW_FILE_NAME(Raw.file.name)=be-O61.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bf-H5	Genotype:HFD | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=3.5; RAW_FILE_NAME(Raw.file.name)=bf-H5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bf-O51	Genotype:ob_ob | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4; RAW_FILE_NAME(Raw.file.name)=bf-O51.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bf-O56	Genotype:ob_ob | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4.5; RAW_FILE_NAME(Raw.file.name)=bf-O56.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bf-O57	Genotype:ob_ob | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4; RAW_FILE_NAME(Raw.file.name)=bf-O57.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bg-H14	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=345.03; infusion_uL_perMin=4.5; RAW_FILE_NAME(Raw.file.name)=bg-H14.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bg-H19	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=345.03; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=bg-H19.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bg-H5	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=345.03; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=bg-H5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bg-H7	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=345.03; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=bg-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bh-H14	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=bh-H14.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bh-H5	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=bh-H5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bh-H7	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=bh-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bt-H100	Genotype:HFD | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=17; RAW_FILE_NAME(Raw.file.name)=bt-H100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bt-H101	Genotype:HFD | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=14.2; RAW_FILE_NAME(Raw.file.name)=bt-H101.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bt-H102	Genotype:HFD | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=14.2; RAW_FILE_NAME(Raw.file.name)=bt-H102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bt-L130	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=8.6; RAW_FILE_NAME(Raw.file.name)=bt-L130.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bt-L131	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=9.8; RAW_FILE_NAME(Raw.file.name)=bt-L131.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bt-O100	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=20; RAW_FILE_NAME(Raw.file.name)=bt-O100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bt-O101	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=20.7; RAW_FILE_NAME(Raw.file.name)=bt-O101.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bt-O102	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=18.7; RAW_FILE_NAME(Raw.file.name)=bt-O102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bu-H100	Genotype:HFD | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=17.7; RAW_FILE_NAME(Raw.file.name)=bu-H100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bu-H101	Genotype:HFD | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=14.6; RAW_FILE_NAME(Raw.file.name)=bu-H101.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bu-H102	Genotype:HFD | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=14.6; RAW_FILE_NAME(Raw.file.name)=bu-H102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bu-L130	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=9.6; RAW_FILE_NAME(Raw.file.name)=bu-L130.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bu-L131	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=10.5; RAW_FILE_NAME(Raw.file.name)=bu-L131.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bu-O100	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=21; RAW_FILE_NAME(Raw.file.name)=bu-O100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bu-O101	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=22.1; RAW_FILE_NAME(Raw.file.name)=bu-O101.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bu-O102	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=20.7; RAW_FILE_NAME(Raw.file.name)=bu-O102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bw-H103	Genotype:HFD | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=16.9; RAW_FILE_NAME(Raw.file.name)=bw-H103.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bw-H104	Genotype:HFD | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=18; RAW_FILE_NAME(Raw.file.name)=bw-H104.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bw-H105	Genotype:HFD | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=15; RAW_FILE_NAME(Raw.file.name)=bw-H105.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bw-L132	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=10.3; RAW_FILE_NAME(Raw.file.name)=bw-L132.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bw-L133	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=10; RAW_FILE_NAME(Raw.file.name)=bw-L133.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bw-O103	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=17.4; RAW_FILE_NAME(Raw.file.name)=bw-O103.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bw-O104	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 2	tracer_conc_mM=11.45; infusion_uL_perMin=20.2; RAW_FILE_NAME(Raw.file.name)=bw-O104.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bz-tail-H100	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=bz-tail-H100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bz-tail-H101	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=4.5; RAW_FILE_NAME(Raw.file.name)=bz-tail-H101.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bz-tail-H102	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=4.7; RAW_FILE_NAME(Raw.file.name)=bz-tail-H102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bz-tail-L130	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=2.8; RAW_FILE_NAME(Raw.file.name)=bz-tail-L130.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bz-tail-L131	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=3.2; RAW_FILE_NAME(Raw.file.name)=bz-tail-L131.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bz-tail-L133	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=bz-tail-L133.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bz-tail-O100	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=bz-tail-O100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bz-tail-O101	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=bz-tail-O101.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bz-tail-O102	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=bz-tail-O102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	c-art-L2	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.43; RAW_FILE_NAME(Raw.file.name)=c-art-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	c-art-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=3.672; RAW_FILE_NAME(Raw.file.name)=c-art-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	c-tail-L2	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.43; RAW_FILE_NAME(Raw.file.name)=c-tail-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	c-tail-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=3.672; RAW_FILE_NAME(Raw.file.name)=c-tail-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	c-tail-O4	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=3.824; RAW_FILE_NAME(Raw.file.name)=c-tail-O4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ca-tail-H100	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=ca-tail-H100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ca-tail-H101	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=4.23; RAW_FILE_NAME(Raw.file.name)=ca-tail-H101.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ca-tail-H102	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=4.72; RAW_FILE_NAME(Raw.file.name)=ca-tail-H102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ca-tail-L130	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=2.82; RAW_FILE_NAME(Raw.file.name)=ca-tail-L130.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ca-tail-L131	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=3.21; RAW_FILE_NAME(Raw.file.name)=ca-tail-L131.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ca-tail-L133	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=3.18; RAW_FILE_NAME(Raw.file.name)=ca-tail-L133.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ca-tail-O100	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=5.5; RAW_FILE_NAME(Raw.file.name)=ca-tail-O100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ca-tail-O101	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=5.5; RAW_FILE_NAME(Raw.file.name)=ca-tail-O101.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ca-tail-O102	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=43.21; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=ca-tail-O102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ce-H102	Genotype:HFD | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=18.9; RAW_FILE_NAME(Raw.file.name)=ce-H102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ce-H106	Genotype:HFD | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=17.9; RAW_FILE_NAME(Raw.file.name)=ce-H106.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ce-H107	Genotype:HFD | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=15.9; RAW_FILE_NAME(Raw.file.name)=ce-H107.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ce-L130	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=10.3; RAW_FILE_NAME(Raw.file.name)=ce-L130.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ce-L140	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=13.2; RAW_FILE_NAME(Raw.file.name)=ce-L140.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ce-O100	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=19; RAW_FILE_NAME(Raw.file.name)=ce-O100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ce-O102	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=19; RAW_FILE_NAME(Raw.file.name)=ce-O102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ce-O106	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=18; RAW_FILE_NAME(Raw.file.name)=ce-O106.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cf-tail-H102	Genotype:HFD | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=19; RAW_FILE_NAME(Raw.file.name)=cf-tail-H102.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cf-tail-H106	Genotype:HFD | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=16; RAW_FILE_NAME(Raw.file.name)=cf-tail-H106.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cf-tail-H107	Genotype:HFD | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=17; RAW_FILE_NAME(Raw.file.name)=cf-tail-H107.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cf-tail-L130	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=10; RAW_FILE_NAME(Raw.file.name)=cf-tail-L130.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cf-tail-L140	Genotype:wild_type | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=13; RAW_FILE_NAME(Raw.file.name)=cf-tail-L140.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cf-tail-O100	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=22; RAW_FILE_NAME(Raw.file.name)=cf-tail-O100.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cf-tail-O106	Genotype:ob_ob | Sample source:blood | Treatment:13C_C18 1	tracer_conc_mM=9.13; infusion_uL_perMin=22; RAW_FILE_NAME(Raw.file.name)=cf-tail-O106.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cg-tail-H108	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.5; infusion_uL_perMin=4; RAW_FILE_NAME(Raw.file.name)=cg-tail-H108.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cg-tail-H109	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.5; infusion_uL_perMin=4; RAW_FILE_NAME(Raw.file.name)=cg-tail-H109.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cg-tail-H110	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.5; infusion_uL_perMin=4; RAW_FILE_NAME(Raw.file.name)=cg-tail-H110.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cg-tail-L141	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.5; infusion_uL_perMin=3.02; RAW_FILE_NAME(Raw.file.name)=cg-tail-L141.mzXML
SUBJECT_SAMPLE_FACTORS           	-	cg-tail-L142	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=206.5; infusion_uL_perMin=3.02; RAW_FILE_NAME(Raw.file.name)=cg-tail-L142.mzXML
SUBJECT_SAMPLE_FACTORS           	-	da-tail-H2	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=8.2; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=da-tail-H2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	da-tail-H4	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=8.2; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=da-tail-H4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	da-tail-H6	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=8.2; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=da-tail-H6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	da-tail-H9	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=8.2; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=da-tail-H9.mzXML
SUBJECT_SAMPLE_FACTORS           	-	da-tail-L1	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=8.2; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=da-tail-L1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	da-tail-L8	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=8.2; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=da-tail-L8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	da-tail-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=8.2; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=da-tail-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	da-tail-O7	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=8.2; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=da-tail-O7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	f-art-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.72; RAW_FILE_NAME(Raw.file.name)=f-art-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	f-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.66; RAW_FILE_NAME(Raw.file.name)=f-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	f-art-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=3.936; RAW_FILE_NAME(Raw.file.name)=f-art-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	f-art-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=3.504; RAW_FILE_NAME(Raw.file.name)=f-art-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	f-tail-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.72; RAW_FILE_NAME(Raw.file.name)=f-tail-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	f-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.66; RAW_FILE_NAME(Raw.file.name)=f-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	f-tail-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=3.936; RAW_FILE_NAME(Raw.file.name)=f-tail-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	f-tail-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=3.504; RAW_FILE_NAME(Raw.file.name)=f-tail-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	g-art-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.46; RAW_FILE_NAME(Raw.file.name)=g-art-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	g-art-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=3.992; RAW_FILE_NAME(Raw.file.name)=g-art-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	g-tail-L2	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.44; RAW_FILE_NAME(Raw.file.name)=g-tail-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	g-tail-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.46; RAW_FILE_NAME(Raw.file.name)=g-tail-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	g-tail-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=3.992; RAW_FILE_NAME(Raw.file.name)=g-tail-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	g-tail-O4	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=3.832; RAW_FILE_NAME(Raw.file.name)=g-tail-O4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	h-art-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.87; RAW_FILE_NAME(Raw.file.name)=h-art-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	h-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.76; RAW_FILE_NAME(Raw.file.name)=h-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	h-art-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=4.376; RAW_FILE_NAME(Raw.file.name)=h-art-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	h-art-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=3.736; RAW_FILE_NAME(Raw.file.name)=h-art-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	h-tail-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.87; RAW_FILE_NAME(Raw.file.name)=h-tail-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	h-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.76; RAW_FILE_NAME(Raw.file.name)=h-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	h-tail-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=4.376; RAW_FILE_NAME(Raw.file.name)=h-tail-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	h-tail-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=3.736; RAW_FILE_NAME(Raw.file.name)=h-tail-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	i-art-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=2.41; RAW_FILE_NAME(Raw.file.name)=i-art-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	i-art-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=4.056; RAW_FILE_NAME(Raw.file.name)=i-art-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	i-tail-L2	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=2.49; RAW_FILE_NAME(Raw.file.name)=i-tail-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	i-tail-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=2.41; RAW_FILE_NAME(Raw.file.name)=i-tail-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	i-tail-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=4.056; RAW_FILE_NAME(Raw.file.name)=i-tail-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	i-tail-O4	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=3.832; RAW_FILE_NAME(Raw.file.name)=i-tail-O4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	k-art-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=2.89; RAW_FILE_NAME(Raw.file.name)=k-art-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	k-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=2.74; RAW_FILE_NAME(Raw.file.name)=k-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	k-art-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=4.416; RAW_FILE_NAME(Raw.file.name)=k-art-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	k-art-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=3.8; RAW_FILE_NAME(Raw.file.name)=k-art-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	k-tail-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=2.89; RAW_FILE_NAME(Raw.file.name)=k-tail-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	k-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=2.74; RAW_FILE_NAME(Raw.file.name)=k-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	k-tail-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=4.416; RAW_FILE_NAME(Raw.file.name)=k-tail-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	k-tail-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=3.8; RAW_FILE_NAME(Raw.file.name)=k-tail-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	m-art-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3.396; RAW_FILE_NAME(Raw.file.name)=m-art-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	m-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3.252; RAW_FILE_NAME(Raw.file.name)=m-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	m-art-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=5.74; RAW_FILE_NAME(Raw.file.name)=m-art-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	m-art-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=4.9; RAW_FILE_NAME(Raw.file.name)=m-art-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	m-tail-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3.396; RAW_FILE_NAME(Raw.file.name)=m-tail-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	m-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3.252; RAW_FILE_NAME(Raw.file.name)=m-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	m-tail-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=5.74; RAW_FILE_NAME(Raw.file.name)=m-tail-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	m-tail-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=4.9; RAW_FILE_NAME(Raw.file.name)=m-tail-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	n-art-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=3.872; RAW_FILE_NAME(Raw.file.name)=n-art-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	n-tail-L2	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.6; RAW_FILE_NAME(Raw.file.name)=n-tail-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	n-tail-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=2.52; RAW_FILE_NAME(Raw.file.name)=n-tail-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	n-tail-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=4.528; RAW_FILE_NAME(Raw.file.name)=n-tail-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	n-tail-O3	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=3.872; RAW_FILE_NAME(Raw.file.name)=n-tail-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	n-tail-O4	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=199.8; infusion_uL_perMin=3.84; RAW_FILE_NAME(Raw.file.name)=n-tail-O4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	p-art-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=2.77; RAW_FILE_NAME(Raw.file.name)=p-art-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	p-art-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=2.49; RAW_FILE_NAME(Raw.file.name)=p-art-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	p-tail-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=2.77; RAW_FILE_NAME(Raw.file.name)=p-tail-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	p-tail-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=2.49; RAW_FILE_NAME(Raw.file.name)=p-tail-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	p-tail-O7	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.1; infusion_uL_perMin=4.2; RAW_FILE_NAME(Raw.file.name)=p-tail-O7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=2.65; RAW_FILE_NAME(Raw.file.name)=r-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-art-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=4.67; RAW_FILE_NAME(Raw.file.name)=r-art-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=2.65; RAW_FILE_NAME(Raw.file.name)=r-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-tail-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=5.92; RAW_FILE_NAME(Raw.file.name)=r-tail-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-tail-O5	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=4.83; RAW_FILE_NAME(Raw.file.name)=r-tail-O5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-tail-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=4.67; RAW_FILE_NAME(Raw.file.name)=r-tail-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	s-art-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=2.62; RAW_FILE_NAME(Raw.file.name)=s-art-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	s-art-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=2.48; RAW_FILE_NAME(Raw.file.name)=s-art-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	s-tail-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=2.62; RAW_FILE_NAME(Raw.file.name)=s-tail-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	s-tail-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=2.48; RAW_FILE_NAME(Raw.file.name)=s-tail-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=2.64; RAW_FILE_NAME(Raw.file.name)=u-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-art-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=6.01; RAW_FILE_NAME(Raw.file.name)=u-art-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-art-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=4.95; RAW_FILE_NAME(Raw.file.name)=u-art-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=2.64; RAW_FILE_NAME(Raw.file.name)=u-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-tail-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=6.01; RAW_FILE_NAME(Raw.file.name)=u-tail-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-tail-O4	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=4.22; RAW_FILE_NAME(Raw.file.name)=u-tail-O4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-tail-O5	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=5.03; RAW_FILE_NAME(Raw.file.name)=u-tail-O5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-tail-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=4.95; RAW_FILE_NAME(Raw.file.name)=u-tail-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	w-art-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=2.73; RAW_FILE_NAME(Raw.file.name)=w-art-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	w-art-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=2.56; RAW_FILE_NAME(Raw.file.name)=w-art-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	w-art-O7	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=3.753; RAW_FILE_NAME(Raw.file.name)=w-art-O7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	w-tail-L4	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=2.73; RAW_FILE_NAME(Raw.file.name)=w-tail-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	w-tail-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=2.56; RAW_FILE_NAME(Raw.file.name)=w-tail-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	w-tail-O7	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=3.753; RAW_FILE_NAME(Raw.file.name)=w-tail-O7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	x-tail-L2	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.1; RAW_FILE_NAME(Raw.file.name)=x-tail-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	x-tail-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.002; RAW_FILE_NAME(Raw.file.name)=x-tail-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	x-tail-O1	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.223; RAW_FILE_NAME(Raw.file.name)=x-tail-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-art-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.53; RAW_FILE_NAME(Raw.file.name)=y-art-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-art-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.936; RAW_FILE_NAME(Raw.file.name)=y-art-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-art-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.288; RAW_FILE_NAME(Raw.file.name)=y-art-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-tail-L5	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.53; RAW_FILE_NAME(Raw.file.name)=y-tail-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-tail-O2	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.936; RAW_FILE_NAME(Raw.file.name)=y-tail-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-tail-O5	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.328; RAW_FILE_NAME(Raw.file.name)=y-tail-O5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-tail-O6	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.288; RAW_FILE_NAME(Raw.file.name)=y-tail-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	z-art-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.53; RAW_FILE_NAME(Raw.file.name)=z-art-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	z-tail-L2	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.72; RAW_FILE_NAME(Raw.file.name)=z-tail-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	z-tail-L6	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.62; RAW_FILE_NAME(Raw.file.name)=z-tail-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	z-tail-L7	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.53; RAW_FILE_NAME(Raw.file.name)=z-tail-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T0-1	Genotype:wild_type | Sample source:blood | Treatment:basal	tracer_conc_mM=-; infusion_uL_perMin=-; RAW_FILE_NAME(Raw.file.name)=T0-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T1-1	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose	tracer_conc_mM=200; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=T1-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T2-1	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose and 13C-palmitate	tracer_conc_mM=200 and 8; infusion_uL_perMin=3 and 6; RAW_FILE_NAME(Raw.file.name)=T2-1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T0-2	Genotype:wild_type | Sample source:blood | Treatment:basal	tracer_conc_mM=-; infusion_uL_perMin=-; RAW_FILE_NAME(Raw.file.name)=T0-2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T1-2	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose	tracer_conc_mM=200; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=T1-2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T2-2	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose and 13C-palmitate	tracer_conc_mM=200 and 8; infusion_uL_perMin=3 and 6; RAW_FILE_NAME(Raw.file.name)=T2-2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T0-3	Genotype:wild_type | Sample source:blood | Treatment:basal	tracer_conc_mM=-; infusion_uL_perMin=-; RAW_FILE_NAME(Raw.file.name)=T0-3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T1-3	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose	tracer_conc_mM=200; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=T1-3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T2-3	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose and 13C-palmitate	tracer_conc_mM=200 and 8; infusion_uL_perMin=3 and 6; RAW_FILE_NAME(Raw.file.name)=T2-3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T0-4	Genotype:wild_type | Sample source:blood | Treatment:basal	tracer_conc_mM=-; infusion_uL_perMin=-; RAW_FILE_NAME(Raw.file.name)=T0-4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T1-4	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose	tracer_conc_mM=200; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=T1-4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T2-4	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose and 13C-palmitate	tracer_conc_mM=200 and 8; infusion_uL_perMin=3 and 6; RAW_FILE_NAME(Raw.file.name)=T2-4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T0-5	Genotype:wild_type | Sample source:blood | Treatment:basal	tracer_conc_mM=-; infusion_uL_perMin=-; RAW_FILE_NAME(Raw.file.name)=T0-5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T1-5	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose	tracer_conc_mM=200; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=T1-5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	T2-5	Genotype:wild_type | Sample source:blood | Treatment:hyperinsulinemia, 13C-glucose and 13C-palmitate	tracer_conc_mM=200 and 8; infusion_uL_perMin=3 and 6; RAW_FILE_NAME(Raw.file.name)=T2-5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	aa-L8 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.71; RAW_FILE_NAME(Raw.file.name)=aa-L8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	aa-O3 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=3.824; RAW_FILE_NAME(Raw.file.name)=aa-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	aa-O7 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=3.096; RAW_FILE_NAME(Raw.file.name)=aa-O7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	aa-O8 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=3.872; RAW_FILE_NAME(Raw.file.name)=aa-O8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-L5 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.63; RAW_FILE_NAME(Raw.file.name)=ab-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-O2 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=4.432; RAW_FILE_NAME(Raw.file.name)=ab-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-O5 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=4.432; RAW_FILE_NAME(Raw.file.name)=ab-O5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ab-O6 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=4.464; RAW_FILE_NAME(Raw.file.name)=ab-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ac-L2 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=2.8; RAW_FILE_NAME(Raw.file.name)=ac-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ac-L6 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=2.63; RAW_FILE_NAME(Raw.file.name)=ac-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ac-O1 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=ac-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ad-L7 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.124; RAW_FILE_NAME(Raw.file.name)=ad-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ad-O4 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=403.224; infusion_uL_perMin=2.96; RAW_FILE_NAME(Raw.file.name)=ad-O4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ad-O8 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=447.002; infusion_uL_perMin=3.664; RAW_FILE_NAME(Raw.file.name)=ad-O8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ae-L5 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=2.53; RAW_FILE_NAME(Raw.file.name)=ae-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ae-L7 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=2.58; RAW_FILE_NAME(Raw.file.name)=ae-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ae-O2 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=5.62; RAW_FILE_NAME(Raw.file.name)=ae-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ae-O3 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=3.96; RAW_FILE_NAME(Raw.file.name)=ae-O3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ae-O6 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=5.62; RAW_FILE_NAME(Raw.file.name)=ae-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	am-O31 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.3; infusion_uL_perMin=5.53; RAW_FILE_NAME(Raw.file.name)=am-O31.mzXML
SUBJECT_SAMPLE_FACTORS           	-	am-O33 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.3; infusion_uL_perMin=5.76; RAW_FILE_NAME(Raw.file.name)=am-O33.mzXML
SUBJECT_SAMPLE_FACTORS           	-	am-O34 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.3; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=am-O34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	an-O31 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=10.9; RAW_FILE_NAME(Raw.file.name)=an-O31.mzXML
SUBJECT_SAMPLE_FACTORS           	-	an-O33 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=11.46; RAW_FILE_NAME(Raw.file.name)=an-O33.mzXML
SUBJECT_SAMPLE_FACTORS           	-	an-O34 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=11.78; RAW_FILE_NAME(Raw.file.name)=an-O34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-L32 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=5.26; RAW_FILE_NAME(Raw.file.name)=ao-L32.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-L33 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=5.02; RAW_FILE_NAME(Raw.file.name)=ao-L33.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-L34 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=5.42; RAW_FILE_NAME(Raw.file.name)=ao-L34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-L35 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=5.46; RAW_FILE_NAME(Raw.file.name)=ao-L35.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-O31 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=11.04; RAW_FILE_NAME(Raw.file.name)=ao-O31.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-O32 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=12.06; RAW_FILE_NAME(Raw.file.name)=ao-O32.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-O34 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=11.82; RAW_FILE_NAME(Raw.file.name)=ao-O34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ao-O35 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=12.06; RAW_FILE_NAME(Raw.file.name)=ao-O35.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ap-L32 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=2.56; RAW_FILE_NAME(Raw.file.name)=ap-L32.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ap-O32 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=5.2; RAW_FILE_NAME(Raw.file.name)=ap-O32.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ap-O33 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=5.62; RAW_FILE_NAME(Raw.file.name)=ap-O33.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ap-O34 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=110.13; infusion_uL_perMin=5.88; RAW_FILE_NAME(Raw.file.name)=ap-O34.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ar-H1 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=4.45; RAW_FILE_NAME(Raw.file.name)=ar-H1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ar-L66 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.01; RAW_FILE_NAME(Raw.file.name)=ar-L66.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ar-O60 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=ar-O60.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ar-O61 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=ar-O61.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-H3 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=4.1; RAW_FILE_NAME(Raw.file.name)=as-H3.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-H4 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.93; RAW_FILE_NAME(Raw.file.name)=as-H4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-L61 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.1; RAW_FILE_NAME(Raw.file.name)=as-L61.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-O53 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=as-O53.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-O54 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=as-O54.mzXML
SUBJECT_SAMPLE_FACTORS           	-	as-O58 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=as-O58.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-H1 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=4.54; RAW_FILE_NAME(Raw.file.name)=at-H1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-H4 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=3.94; RAW_FILE_NAME(Raw.file.name)=at-H4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-L62 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=3.31; RAW_FILE_NAME(Raw.file.name)=at-L62.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-L63 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=4.31; RAW_FILE_NAME(Raw.file.name)=at-L63.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-O51 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=at-O51.mzXML
SUBJECT_SAMPLE_FACTORS           	-	at-O56 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=5.7; RAW_FILE_NAME(Raw.file.name)=at-O56.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H10 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.06; RAW_FILE_NAME(Raw.file.name)=az-H10.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H11 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.91; RAW_FILE_NAME(Raw.file.name)=az-H11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H7 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.48; RAW_FILE_NAME(Raw.file.name)=az-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H8 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.69; RAW_FILE_NAME(Raw.file.name)=az-H8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-H9 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.68; RAW_FILE_NAME(Raw.file.name)=az-H9.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-L63 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=4.25; RAW_FILE_NAME(Raw.file.name)=az-L63.mzXML
SUBJECT_SAMPLE_FACTORS           	-	az-O58 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.8; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=az-O58.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-H1 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.5; RAW_FILE_NAME(Raw.file.name)=ba-H1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-H4 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.46; RAW_FILE_NAME(Raw.file.name)=ba-H4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-H5 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.5; RAW_FILE_NAME(Raw.file.name)=ba-H5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-H6 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.7; RAW_FILE_NAME(Raw.file.name)=ba-H6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-L66 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.1; RAW_FILE_NAME(Raw.file.name)=ba-L66.mzXML
SUBJECT_SAMPLE_FACTORS           	-	ba-O61 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=62.52; infusion_uL_perMin=3.5; RAW_FILE_NAME(Raw.file.name)=ba-O61.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H10 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.05; RAW_FILE_NAME(Raw.file.name)=bb-H10.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H11 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.91; RAW_FILE_NAME(Raw.file.name)=bb-H11.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H13 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=3.94; RAW_FILE_NAME(Raw.file.name)=bb-H13.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H14 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.38; RAW_FILE_NAME(Raw.file.name)=bb-H14.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H7 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.46; RAW_FILE_NAME(Raw.file.name)=bb-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H8 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.68; RAW_FILE_NAME(Raw.file.name)=bb-H8.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bb-H9 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	tracer_conc_mM=109.4; infusion_uL_perMin=4.58; RAW_FILE_NAME(Raw.file.name)=bb-H9.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bc-H15 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=8.92; RAW_FILE_NAME(Raw.file.name)=bc-H15.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bc-H17 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=9.9; RAW_FILE_NAME(Raw.file.name)=bc-H17.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bc-H18 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=8.02; RAW_FILE_NAME(Raw.file.name)=bc-H18.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bc-L62 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	tracer_conc_mM=10.68; infusion_uL_perMin=6.46; RAW_FILE_NAME(Raw.file.name)=bc-L62.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bd-H10 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4.18; RAW_FILE_NAME(Raw.file.name)=bd-H10.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bd-H7 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=4.72; RAW_FILE_NAME(Raw.file.name)=bd-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bd-L64 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Valine	tracer_conc_mM=20.4; infusion_uL_perMin=2.99; RAW_FILE_NAME(Raw.file.name)=bd-L64.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bg-H14 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=345.03; infusion_uL_perMin=4.5; RAW_FILE_NAME(Raw.file.name)=bg-H14.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bg-H19 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=345.03; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=bg-H19.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bg-H7 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	tracer_conc_mM=345.03; infusion_uL_perMin=5; RAW_FILE_NAME(Raw.file.name)=bg-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bh-H14 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=bh-H14.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bh-H19 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=bh-H19.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bh-H5 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=bh-H5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bh-H7 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	tracer_conc_mM=85.9; infusion_uL_perMin=3; RAW_FILE_NAME(Raw.file.name)=bh-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bi-H5 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=4.5; RAW_FILE_NAME(Raw.file.name)=bi-H5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bi-H51 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=4; RAW_FILE_NAME(Raw.file.name)=bi-H51.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bi-H52 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=4; RAW_FILE_NAME(Raw.file.name)=bi-H52.mzXML
SUBJECT_SAMPLE_FACTORS           	-	bi-H7 (glycerol anal.)	Genotype:HFD | Sample source:blood | Treatment:13C_Alanine	tracer_conc_mM=103.74; infusion_uL_perMin=4.6; RAW_FILE_NAME(Raw.file.name)=bi-H7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-L5 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=2.65; RAW_FILE_NAME(Raw.file.name)=r-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-O2 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=5.92; RAW_FILE_NAME(Raw.file.name)=r-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-O5 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=4.83; RAW_FILE_NAME(Raw.file.name)=r-O5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	r-O6 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=4.67; RAW_FILE_NAME(Raw.file.name)=r-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	s-L4 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=2.62; RAW_FILE_NAME(Raw.file.name)=s-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	s-L7 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	tracer_conc_mM=61.5; infusion_uL_perMin=2.48; RAW_FILE_NAME(Raw.file.name)=s-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-L5 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=2.64; RAW_FILE_NAME(Raw.file.name)=u-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-O2 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=6.01; RAW_FILE_NAME(Raw.file.name)=u-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-O4 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=4.22; RAW_FILE_NAME(Raw.file.name)=u-O4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-O5 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=5.03; RAW_FILE_NAME(Raw.file.name)=u-O5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	u-O6 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=4.95; RAW_FILE_NAME(Raw.file.name)=u-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	w-L4 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=2.73; RAW_FILE_NAME(Raw.file.name)=w-L4.mzXML
SUBJECT_SAMPLE_FACTORS           	-	w-L7 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=2.56; RAW_FILE_NAME(Raw.file.name)=w-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	w-O7 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	tracer_conc_mM=100.8; infusion_uL_perMin=3.753; RAW_FILE_NAME(Raw.file.name)=w-O7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	x-L2 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.1; RAW_FILE_NAME(Raw.file.name)=x-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	x-L6 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.002; RAW_FILE_NAME(Raw.file.name)=x-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	x-O1 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.223; RAW_FILE_NAME(Raw.file.name)=x-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-L5 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.53; RAW_FILE_NAME(Raw.file.name)=y-L5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-O2 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.936; RAW_FILE_NAME(Raw.file.name)=y-O2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-O5 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.328; RAW_FILE_NAME(Raw.file.name)=y-O5.mzXML
SUBJECT_SAMPLE_FACTORS           	-	y-O6 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.288; RAW_FILE_NAME(Raw.file.name)=y-O6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	z-L2 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.72; RAW_FILE_NAME(Raw.file.name)=z-L2.mzXML
SUBJECT_SAMPLE_FACTORS           	-	z-L6 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.62; RAW_FILE_NAME(Raw.file.name)=z-L6.mzXML
SUBJECT_SAMPLE_FACTORS           	-	z-L7 (glycerol anal.)	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=2.53; RAW_FILE_NAME(Raw.file.name)=z-L7.mzXML
SUBJECT_SAMPLE_FACTORS           	-	z-O1 (glycerol anal.)	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	tracer_conc_mM=200.3; infusion_uL_perMin=4.48; RAW_FILE_NAME(Raw.file.name)=z-O1.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M1-0	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M1-0.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M6-0	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M6-0.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M8-0	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M8-0.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M9-0	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M9-0.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M1-15	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M1-15.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M6-15	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M6-15.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M8-15	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M8-15.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M9-15	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M9-15.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M1-30	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M1-30.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M6-30	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M6-30.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M8-30	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M8-30.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M9-30	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M9-30.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M1-45	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M1-45.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M6-45	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M6-45.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M8-45	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M8-45.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M9-45	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M9-45.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M1-60	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M1-60.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M6-60	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M6-60.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M8-60	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M8-60.mzXML
SUBJECT_SAMPLE_FACTORS           	-	M9-60	Genotype:wild_type | Sample source:blood | Treatment:13C-C16 0	tracer_conc_mM=8; infusion_uL_perMin=6; RAW_FILE_NAME(Raw.file.name)=M9-60.mzXML
#COLLECTION
CO:COLLECTION_SUMMARY            	Blood was collected by tail snip, or from implanted carotid artery catheter.
CO:SAMPLE_TYPE                   	Blood (serum)
CO:STORAGE_CONDITIONS            	-80℃
#TREATMENT
TR:TREATMENT_SUMMARY             	Treatment #1 Infusion of Isotope Tracers with serum metabolites labeling
TR:TREATMENT_SUMMARY             	measurement. On the day of tracer infusion, the mouse was transferred to a new
TR:TREATMENT_SUMMARY             	cage without food at 9 AM, and fasted for 4 hours before infusion. Right before
TR:TREATMENT_SUMMARY             	the start of infusion, the lock solution was drawn and removed from the
TR:TREATMENT_SUMMARY             	in-dwelling jugular vein catheter, and the catheter was flushed with 50 µL
TR:TREATMENT_SUMMARY             	saline. The vascular access button was then connected to the infusion tether,
TR:TREATMENT_SUMMARY             	which was in turn connected with a swivel on a counter-balance arm. This allowed
TR:TREATMENT_SUMMARY             	the mouse to move freely around in the cage. The infusion of U-13C-tracer at a
TR:TREATMENT_SUMMARY             	small non-perturbing dose started at 2 PM, and lasted for 30 min for fatty acids
TR:TREATMENT_SUMMARY             	and 2.5-3 h for all other tracers. The tracer concentration and infusion rates
TR:TREATMENT_SUMMARY             	can be found in the Study Design section. At the end of infusion, 40 µL blood
TR:TREATMENT_SUMMARY             	was collected by tail snip. For infusion of 13C-lactate, alanine, and glutamine,
TR:TREATMENT_SUMMARY             	given their significant labeling difference in the arterial and venous blood, 40
TR:TREATMENT_SUMMARY             	µL arterial blood was sampled from the carotid artery via the pre-implanted
TR:TREATMENT_SUMMARY             	catheter, and used for analysis. The blood was collected into a Microvette tube
TR:TREATMENT_SUMMARY             	with clotting activator, and was kept on ice during collection. After infusion,
TR:TREATMENT_SUMMARY             	the catheter was flushed with 50 µL saline, and then sealed with 10 ~ 20 µL
TR:TREATMENT_SUMMARY             	lock solution. The blood was centrifuged at 5,000 ×g for 10 min. 4 µL serum
TR:TREATMENT_SUMMARY             	was aliquoted in separate tubes, and stored at -80°C. --- Treatment #2
TR:TREATMENT_SUMMARY             	Hyperinsulinemia Clamp. C57BL/6J mouse (26.5 ± 1.8 g) was transferred to a new
TR:TREATMENT_SUMMARY             	cage without food at 9 AM. After fasting for 4 hours, blood was collected via
TR:TREATMENT_SUMMARY             	tail snip for basal glycemia and insulin measurement (noted as time point 0, or
TR:TREATMENT_SUMMARY             	T0). A mixture of U-13C-glucose (200 mM), unlabeled glucose (2361 mM) and
TR:TREATMENT_SUMMARY             	insulin (66.6 mU/mL) was infused at 3 µL/min/mouse. After 2.5 h, blood was
TR:TREATMENT_SUMMARY             	collected for serum glucose labeling and insulin measurement (noted as time
TR:TREATMENT_SUMMARY             	point T1). Then U-13C palmitate (8 mM) was infused at 6 µL/min/animal for
TR:TREATMENT_SUMMARY             	another 30 min (delivered by a second pump), simultaneously with the glucose and
TR:TREATMENT_SUMMARY             	insulin infusion (in the Study Design section, it is noted as "13C-glucose and
TR:TREATMENT_SUMMARY             	13C-C16" with "200 and 8" for concentration and "3 and 6" for infusion rate).
TR:TREATMENT_SUMMARY             	Blood was collected at the end for serum glucose and palmitate labeling and
TR:TREATMENT_SUMMARY             	insulin measurement (time point T2). Insulin was measured by ELISA Kit following
TR:TREATMENT_SUMMARY             	the vendor’s protocol. ---- Treatment #3 Measurement of Triglyceride
TR:TREATMENT_SUMMARY             	Circulatory Turnover Flux. The serum triglyceride turnover was measured with a
TR:TREATMENT_SUMMARY             	pulse-chase strategy. After fasting for 4 hours, BL/6J mouse was infused with 8
TR:TREATMENT_SUMMARY             	mM U-13C-palmitate at 0.25 µL/min/g for 1-2 hours to label serum triglyceride.
TR:TREATMENT_SUMMARY             	The infusion was stopped, and blood was collected by tail snip every 15 minutes
TR:TREATMENT_SUMMARY             	for one hour to monitor the decay of M+16 labeling L in palmitoyl-containing
TR:TREATMENT_SUMMARY             	triglyceride. An exponential curve was fitted with L=Ae^(-kt) for data points
TR:TREATMENT_SUMMARY             	from 15 to 60 min. 0-15 min was excluded from modeling for clearance of labeling
TR:TREATMENT_SUMMARY             	in free fatty acids. The triglyceride turnover flux R_a was calculated as
TR:TREATMENT_SUMMARY             	R_a=k⋅C⋅V, where C is the total triglyceride concentration in serum, and V
TR:TREATMENT_SUMMARY             	the serum volume of 49 µL/g (Clemons, 1992).
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	For general metabolomics analysis with HILIC-MS method #1 (see MS section), 4
SP:SAMPLEPREP_SUMMARY            	µL serum was mixed with 60 µL -20°C methanol: acetonitrile: water at 40: 40:
SP:SAMPLEPREP_SUMMARY            	20. The sample was vigorously vortexed, and centrifuged at 4°C at 13,000 g for
SP:SAMPLEPREP_SUMMARY            	10 min. 30 µL supernatant was transferred to the HPLC vial from which 5 µL was
SP:SAMPLEPREP_SUMMARY            	injected to the LC-MS. For serum glycerol analysis with HILIC-MS method #2,
SP:SAMPLEPREP_SUMMARY            	serum glycerol was first enzymatically derivatized into glycerol-3-phosphate
SP:SAMPLEPREP_SUMMARY            	before LC-MS analysis. 4 µL serum was mixed with 90 µL freshly made enzyme
SP:SAMPLEPREP_SUMMARY            	solution containing 2 U/mL glycerol kinase, 25 mM Tris-HCl (pH 8.0), 50 mM
SP:SAMPLEPREP_SUMMARY            	sodium chloride, 10 mM magnesium chloride, and 1.5 mM ATP, and incubated at room
SP:SAMPLEPREP_SUMMARY            	temperature for 15 min. The reaction was stopped by the addition of 400 µL
SP:SAMPLEPREP_SUMMARY            	methanol. The sample was vortexed, and centrifuged at 16,000 g for 10 min. The
SP:SAMPLEPREP_SUMMARY            	supernatant was transferred to a new tube, and dried using SpeedVac at 45 °C
SP:SAMPLEPREP_SUMMARY            	for 90 min. 60 µL of methanol:acetonitrile:water at 40:40:20 was added to the
SP:SAMPLEPREP_SUMMARY            	residue, vortexed, and centrifuged at 16,000 g for 10 min. 30 µL supernatant
SP:SAMPLEPREP_SUMMARY            	was transferred to the HPLC vial, from which 5 µL was injected to the LC-MS.
SP:SAMPLEPREP_SUMMARY            	For serum triglyceride analysis with RP-MS method #3, 4 µL serum was mixed with
SP:SAMPLEPREP_SUMMARY            	50 µL extraction buffer of butanol:methanol at 1:1 volume ratio containing 5 mM
SP:SAMPLEPREP_SUMMARY            	ammonium formate. The sample was vigorously vortexed, and centrifuged at 4°C at
SP:SAMPLEPREP_SUMMARY            	13,000 g for 10 min. 30 µL supernatant was carefully taken and transferred to
SP:SAMPLEPREP_SUMMARY            	glass insert of LC/MS vials for LC/MS analysis. SPLASH® LIPIDOMIX internal
SP:SAMPLEPREP_SUMMARY            	standard with serial dilution was spiked in serum for calibration and
SP:SAMPLEPREP_SUMMARY            	triglyceride quantification. In the Data(results) section, "0" means
SP:SAMPLEPREP_SUMMARY            	non-detectable labeling, and NA refers to missing measurement (we did not
SP:SAMPLEPREP_SUMMARY            	measure certain metabolites in certain samples).
SP:PROCESSING_STORAGE_CONDITIONS 	On ice
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	HILIC, similar to HILIC method 1, but focuses on glycerol analysis after serum
CH:CHROMATOGRAPHY_SUMMARY        	glycerol is enzymatically derivatized to glycerol-3-phosphate (G3P). Only eluent
CH:CHROMATOGRAPHY_SUMMARY        	1 min around the retention time of G3P is directed to MS.
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Waters XBridge BEH Amide (100 x 2.1mm,2.5um)
CH:SOLVENT_A                     	95% water/5% acetonitrile; 10 mM ammonium acetate; 10 mM ammonium hydroxide
CH:SOLVENT_B                     	20% water/80% acetonitrile; 10 mM ammonium acetate; 10 mM ammonium hydroxide
CH:FLOW_GRADIENT                 	0 - 3 min, 100% B; 3.2 - 6.2 min, 90% B; 6.5. - 10.5 min, 80% B; 10.7 - 13.5
CH:FLOW_GRADIENT                 	min, 70% B; 13.7 - 16 min, 45% B; and 16.5 - 22 min, 100% B
CH:FLOW_RATE                     	300 uL/min
CH:COLUMN_TEMPERATURE            	25
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	The mass spectrometry condition used was the same as HILIC-MS method (1), except
MS:MS_COMMENTS                   	that only eluent 1 min around the retention time of gycerol-3-phosphate is
MS:MS_COMMENTS                   	directed to MS, otherwise to waste.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	average 13C labeling fraction
MS_METABOLITE_DATA_START
Samples	aa-L8 (glycerol anal.)	aa-O3 (glycerol anal.)	aa-O7 (glycerol anal.)	aa-O8 (glycerol anal.)	ab-L5 (glycerol anal.)	ab-O2 (glycerol anal.)	ab-O5 (glycerol anal.)	ab-O6 (glycerol anal.)	ac-L2 (glycerol anal.)	ac-L6 (glycerol anal.)	ac-O1 (glycerol anal.)	ad-L7 (glycerol anal.)	ad-O4 (glycerol anal.)	ad-O8 (glycerol anal.)	ae-L5 (glycerol anal.)	ae-L7 (glycerol anal.)	ae-O2 (glycerol anal.)	ae-O3 (glycerol anal.)	ae-O6 (glycerol anal.)	am-O31 (glycerol anal.)	am-O33 (glycerol anal.)	am-O34 (glycerol anal.)	an-O31 (glycerol anal.)	an-O33 (glycerol anal.)	an-O34 (glycerol anal.)	ao-L32 (glycerol anal.)	ao-L33 (glycerol anal.)	ao-L34 (glycerol anal.)	ao-L35 (glycerol anal.)	ao-O31 (glycerol anal.)	ao-O32 (glycerol anal.)	ao-O34 (glycerol anal.)	ao-O35 (glycerol anal.)	ap-L32 (glycerol anal.)	ap-O32 (glycerol anal.)	ap-O33 (glycerol anal.)	ap-O34 (glycerol anal.)	ar-H1 (glycerol anal.)	ar-L66 (glycerol anal.)	ar-O60 (glycerol anal.)	ar-O61 (glycerol anal.)	as-H3 (glycerol anal.)	as-H4 (glycerol anal.)	as-L61 (glycerol anal.)	as-O53 (glycerol anal.)	as-O54 (glycerol anal.)	as-O58 (glycerol anal.)	at-H1 (glycerol anal.)	at-H4 (glycerol anal.)	at-L62 (glycerol anal.)	at-L63 (glycerol anal.)	at-O51 (glycerol anal.)	at-O56 (glycerol anal.)	az-H10 (glycerol anal.)	az-H11 (glycerol anal.)	az-H7 (glycerol anal.)	az-H8 (glycerol anal.)	az-H9 (glycerol anal.)	az-L63 (glycerol anal.)	az-O58 (glycerol anal.)	ba-H1 (glycerol anal.)	ba-H4 (glycerol anal.)	ba-H5 (glycerol anal.)	ba-H6 (glycerol anal.)	ba-L66 (glycerol anal.)	ba-O61 (glycerol anal.)	bb-H10 (glycerol anal.)	bb-H11 (glycerol anal.)	bb-H13 (glycerol anal.)	bb-H14 (glycerol anal.)	bb-H7 (glycerol anal.)	bb-H8 (glycerol anal.)	bb-H9 (glycerol anal.)	bc-H15 (glycerol anal.)	bc-H17 (glycerol anal.)	bc-H18 (glycerol anal.)	bc-L62 (glycerol anal.)	bd-H10 (glycerol anal.)	bd-H7 (glycerol anal.)	bd-L64 (glycerol anal.)	bg-H14 (glycerol anal.)	bg-H19 (glycerol anal.)	bg-H7 (glycerol anal.)	bh-H14 (glycerol anal.)	bh-H19 (glycerol anal.)	bh-H5 (glycerol anal.)	bh-H7 (glycerol anal.)	bi-H5 (glycerol anal.)	bi-H51 (glycerol anal.)	bi-H52 (glycerol anal.)	bi-H7 (glycerol anal.)	r-L5 (glycerol anal.)	r-O2 (glycerol anal.)	r-O5 (glycerol anal.)	r-O6 (glycerol anal.)	s-L4 (glycerol anal.)	s-L7 (glycerol anal.)	u-L5 (glycerol anal.)	u-O2 (glycerol anal.)	u-O4 (glycerol anal.)	u-O5 (glycerol anal.)	u-O6 (glycerol anal.)	w-L4 (glycerol anal.)	w-L7 (glycerol anal.)	w-O7 (glycerol anal.)	x-L2 (glycerol anal.)	x-L6 (glycerol anal.)	x-O1 (glycerol anal.)	y-L5 (glycerol anal.)	y-O2 (glycerol anal.)	y-O5 (glycerol anal.)	y-O6 (glycerol anal.)	z-L2 (glycerol anal.)	z-L6 (glycerol anal.)	z-L7 (glycerol anal.)	z-O1 (glycerol anal.)
Factors	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	Genotype:wild_type | Sample source:blood | Treatment:13C_Lactate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Lactate	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	Genotype:wild_type | Sample source:blood | Treatment:13C_Alanine	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	Genotype:ob_ob | Sample source:blood | Treatment:13C_Alanine	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	Genotype:ob_ob | Sample source:blood | Treatment:13C_C16 0	Genotype:wild_type | Sample source:blood | Treatment:13C_Glycerol	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glycerol	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	Genotype:HFD | Sample source:blood | Treatment:13C_Acetate	Genotype:HFD | Sample source:blood | Treatment:13C_Acetate	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	Genotype:HFD | Sample source:blood | Treatment:13C_Glucose	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	Genotype:HFD | Sample source:blood | Treatment:13C_3-HB	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	Genotype:HFD | Sample source:blood | Treatment:13C_Glycerol	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	Genotype:HFD | Sample source:blood | Treatment:13C_C16 0	Genotype:wild_type | Sample source:blood | Treatment:13C_C16 0	Genotype:HFD | Sample source:blood | Treatment:13C_Valine	Genotype:HFD | Sample source:blood | Treatment:13C_Valine	Genotype:wild_type | Sample source:blood | Treatment:13C_Valine	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	Genotype:HFD | Sample source:blood | Treatment:13C_Lactate	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	Genotype:HFD | Sample source:blood | Treatment:13C_Glutamine	Genotype:HFD | Sample source:blood | Treatment:13C_Alanine	Genotype:HFD | Sample source:blood | Treatment:13C_Alanine	Genotype:HFD | Sample source:blood | Treatment:13C_Alanine	Genotype:HFD | Sample source:blood | Treatment:13C_Alanine	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	Genotype:ob_ob | Sample source:blood | Treatment:13C_3-HB	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	Genotype:wild_type | Sample source:blood | Treatment:13C_3-HB	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	Genotype:wild_type | Sample source:blood | Treatment:13C_Acetate	Genotype:ob_ob | Sample source:blood | Treatment:13C_Acetate	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	Genotype:wild_type | Sample source:blood | Treatment:13C_Glucose	Genotype:ob_ob | Sample source:blood | Treatment:13C_Glucose
Glycerol	0.0102416449995535	0.00290328578544866	0.0070496053334082	0.0104504061122554	0.00472944878740935	0.0119850980686571	0.00736924374357093	0.00201145503547429	0.149185651317561	0.118767973629771	0.141248462693852	0.0056864863343344	0.00275370543913931	0.0119610679741249	0.0005777292978424	9.9967417334527e-05	0.00156123127077991	0.000781063087868615	0.000614944920051646	0.187296731856051	0.189316297187904	0.189550902230459	0	0	0	0	0	0	0	0	0	0	0	0.160527383789488	0.174049228331435	0.160459950392984	0.139022094171149	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0.0553544059532314	0.052673586561926	0.0577051078787567	0.0496629857558442	0.0530573717177299	0.0420440786048894	0.0369771099552318	0	0	0	0	0	0	0.179768424955709	0.17704048820515	0.167010859168106	0.18125704419459	0.164302323056435	0.214992024849307	0.183548073265771	0	0	0	0	0	0	0	0.00279973153316398	0.00616458431688748	0.00127420195009584	0	0	0	0	0	0	0	0	0	0	0	0.00318056398257617	0	0	0	0	0	0	0	0	0	0	0.0118286122687529	0.0101712071550897	0.0158343218658395	0.0105565686605199	0.01695568982847	0.0156210069485944	0.0176946499055829	0.00933974887839725	0.00602756610192457	0.00936465261725725	0.00419097966311665
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	KEGG	PubChem
Glycerol	C00116	753
METABOLITES_END
#END