#METABOLOMICS WORKBENCH wanglgene_20241206_105534 DATATRACK_ID:5436 STUDY_ID:ST003623 ANALYSIS_ID:AN005953 PROJECT_ID:PR002208 VERSION 1 CREATED_ON December 12, 2024, 6:30 pm #PROJECT PR:PROJECT_TITLE NRF2 supports non-small cell lung cancer growth independently of PR:PROJECT_TITLE CBP/p300-enhanced glutathione synthesis PR:PROJECT_TYPE MS quantitative analysis PR:PROJECT_SUMMARY Nuclear factor erythroid 2-related factor 2 (NRF2) is a stress responsive PR:PROJECT_SUMMARY transcription factor that is mutationally activated in a subset (~25%) of PR:PROJECT_SUMMARY clinically-aggressive non-small cell lung cancers (NSCLC). Mechanistic insight PR:PROJECT_SUMMARY into drivers of the NRF2 dependency remains poorly understood. Here, we defined PR:PROJECT_SUMMARY a novel NRF2 target gene set linked to NRF2-dependency in cancer cell lines, and PR:PROJECT_SUMMARY observed that a significant portion of these genes is devoid of PR:PROJECT_SUMMARY promoter-proximal NRF2. Using integrated genomic analyses, we characterized PR:PROJECT_SUMMARY extensive NRF2-dependent enhancer RNA (eRNA) synthesis and NRF2-mediated H3K27ac PR:PROJECT_SUMMARY deposition at proximal and distal enhancer regions regulating these genes. While PR:PROJECT_SUMMARY CBP/p300 is a well-validated direct interaction partner of NRF2 with prominent PR:PROJECT_SUMMARY functions at enhancers, we report that this interaction is not required for PR:PROJECT_SUMMARY NRF2-dependent NSCLC cell growth, indicating that NRF2 can sustain sufficient PR:PROJECT_SUMMARY transcriptional activity in the absence of CBP/p300 coactivation. Broad PR:PROJECT_SUMMARY metabolic profiling established a primary role for CBP/p300 in NRF2-dependent PR:PROJECT_SUMMARY accumulation of glutathione and glutathione-related metabolites. While redox PR:PROJECT_SUMMARY homeostasis via enhanced glutathione production is commonly associated with the PR:PROJECT_SUMMARY normal physiological role of NRF2, collectively our results suggest that PR:PROJECT_SUMMARY NRF2-dependent cancer cell growth does not require this enhanced glutathione PR:PROJECT_SUMMARY production. PR:INSTITUTE Genentech Inc. PR:LAST_NAME Wang PR:FIRST_NAME Mike Lingjue PR:ADDRESS 1 DNA Way, South San Francisco, CA 94404 PR:EMAIL wang.mike@gene.com PR:PHONE 650-245-7991 PR:CONTRIBUTORS Ryan J. Conrad, James A. Mondo, Mike Lingjue Wang, Peter S. Liu, Zijuan Lai, PR:CONTRIBUTORS Feroza K Choudhury, Qingling Li, Weng Ruh Wong, James Lee, Frances Shanahan, Eva PR:CONTRIBUTORS Lin, Scott Martin, Joachim Rudolph, John G. Moffat, Dewakar Sangaraju, Wendy PR:CONTRIBUTORS Sandoval, Timothy Sterne-Weiler, Scott A. Foster #STUDY ST:STUDY_TITLE NRF2 supports non-small cell lung cancer growth independently of ST:STUDY_TITLE CBP/p300-enhanced glutathione synthesis: Global metabolomics analysis on A549 ST:STUDY_TITLE cells at different NRF2 status (Part 1 of 3) ST:STUDY_TYPE Untargeted Metabolomics ST:STUDY_SUMMARY This study aims at discovering metabolic changes in A549 cancer cells in the ST:STUDY_SUMMARY presence and absence of NRF2, and a mutant NRF2 genotypes. Metabolites were ST:STUDY_SUMMARY extracted from cell pellets by using an LLE method with ST:STUDY_SUMMARY Methanol/Chloroform/water. The aqueous layer was analyzed by HILIC-HRMS. An ST:STUDY_SUMMARY in-house RT library was used to identify metabolites. Statistical analyses was ST:STUDY_SUMMARY performed to identify statistically significant changes in the metabolism. 35 ST:STUDY_SUMMARY metabolites presented differential abundance between NRF2 knockdown and wildtype ST:STUDY_SUMMARY conditions. In particular, GSH and several glutamate dipeptides were ST:STUDY_SUMMARY significantly depleted upon NRF2 knockdown, in line with the prevailing role of ST:STUDY_SUMMARY NRF2 in controlling GSH biosynthesis. Disruption of additional metabolites ST:STUDY_SUMMARY involved in the PPP (sedoheptulose-7-phosphate, S7P), nucleotide (CMP, IMP) and ST:STUDY_SUMMARY amino acid (kynurenine, homocitrulline) metabolism were also observed upon NRF2 ST:STUDY_SUMMARY knockdown. ST:INSTITUTE Genentech Inc. ST:LAST_NAME Wang ST:FIRST_NAME Mike ST:ADDRESS 1 DNA Way, South San Francisco, CA 94080, USA ST:EMAIL wang.mike@gene.com ST:PHONE 6502457991 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:CELL_STRAIN_DETAILS A549 SU:SPECIES_GROUP Mammals #FACTORS #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Sample01 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox- | Sample Label:luc-1 Cell count (1E6)=27.2; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_01.mzML SUBJECT_SAMPLE_FACTORS - Sample02 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox- | Sample Label:luc-2 Cell count (1E6)=31.1; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_02.mzML SUBJECT_SAMPLE_FACTORS - Sample03 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox- | Sample Label:luc-3 Cell count (1E6)=27.5; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_03.mzML SUBJECT_SAMPLE_FACTORS - Sample04 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox- | Sample Label:luc-4 Cell count (1E6)=28.2; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_04.mzML SUBJECT_SAMPLE_FACTORS - Sample05 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox- | Sample Label:FL-1 Cell count (1E6)=24.4; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_05.mzML SUBJECT_SAMPLE_FACTORS - Sample06 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox- | Sample Label:FL-2 Cell count (1E6)=25.7; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_06.mzML SUBJECT_SAMPLE_FACTORS - Sample07 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox- | Sample Label:FL-3 Cell count (1E6)=24.3; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_07.mzML SUBJECT_SAMPLE_FACTORS - Sample08 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox- | Sample Label:FL-4 Cell count (1E6)=26.5; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_08.mzML SUBJECT_SAMPLE_FACTORS - Sample09 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox- | Sample Label:45-1 Cell count (1E6)=31.7; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_09.mzML SUBJECT_SAMPLE_FACTORS - Sample10 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox- | Sample Label:45-2 Cell count (1E6)=31.6; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_10.mzML SUBJECT_SAMPLE_FACTORS - Sample11 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox- | Sample Label:45-3 Cell count (1E6)=27.9; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_11.mzML SUBJECT_SAMPLE_FACTORS - Sample12 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox- | Sample Label:45-4 Cell count (1E6)=30.2; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_12.mzML SUBJECT_SAMPLE_FACTORS - Sample17 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+ | Sample Label:luc+1 Cell count (1E6)=22; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_17.mzML SUBJECT_SAMPLE_FACTORS - Sample18 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+ | Sample Label:luc+2 Cell count (1E6)=23; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_18.mzML SUBJECT_SAMPLE_FACTORS - Sample19 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+ | Sample Label:luc+3 Cell count (1E6)=22.8; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_19.mzML SUBJECT_SAMPLE_FACTORS - Sample20 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+ | Sample Label:luc+4 Cell count (1E6)=25.1; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_20.mzML SUBJECT_SAMPLE_FACTORS - Sample21 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox+ | Sample Label:FL+1 Cell count (1E6)=24.4; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_21.mzML SUBJECT_SAMPLE_FACTORS - Sample22 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox+ | Sample Label:FL+2 Cell count (1E6)=22.6; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_22.mzML SUBJECT_SAMPLE_FACTORS - Sample23 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox+ | Sample Label:FL+3 Cell count (1E6)=19.7; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_23.mzML SUBJECT_SAMPLE_FACTORS - Sample24 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox+ | Sample Label:FL+4 Cell count (1E6)=25.1; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_24.mzML SUBJECT_SAMPLE_FACTORS - Sample25 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox+ | Sample Label:45+1 Cell count (1E6)=31.1; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_25.mzML SUBJECT_SAMPLE_FACTORS - Sample26 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox+ | Sample Label:45+2 Cell count (1E6)=28.2; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_26.mzML SUBJECT_SAMPLE_FACTORS - Sample27 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox+ | Sample Label:45+3 Cell count (1E6)=28; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_27.mzML SUBJECT_SAMPLE_FACTORS - Sample28 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox+ | Sample Label:45+4 Cell count (1E6)=28.6; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_28.mzML SUBJECT_SAMPLE_FACTORS - Sample33 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+DMSO | Sample Label:DMSO+1 Cell count (1E6)=35.1; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_33.mzML SUBJECT_SAMPLE_FACTORS - Sample34 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+DMSO | Sample Label:DMSO+2 Cell count (1E6)=33.5; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_34.mzML SUBJECT_SAMPLE_FACTORS - Sample35 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+DMSO | Sample Label:DMSO+3 Cell count (1E6)=30.7; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_35.mzML SUBJECT_SAMPLE_FACTORS - Sample36 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+DMSO | Sample Label:DMSO+4 Cell count (1E6)=30.7; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_36.mzML SUBJECT_SAMPLE_FACTORS - Sample41 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+A485 | Sample Label:A485+1 Cell count (1E6)=35.4; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_41.mzML SUBJECT_SAMPLE_FACTORS - Sample42 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+A485 | Sample Label:A485+2 Cell count (1E6)=32.4; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_42.mzML SUBJECT_SAMPLE_FACTORS - Sample43 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+A485 | Sample Label:A485+3 Cell count (1E6)=31.6; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_43.mzML SUBJECT_SAMPLE_FACTORS - Sample44 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+A485 | Sample Label:A485+4 Cell count (1E6)=29.4; RAW_FILE_NAME(Raw data)=20211026_NRF2_HILIC_Sample_44.mzML #COLLECTION CO:COLLECTION_SUMMARY All cell lines were obtained from the Genentech cell bank (Yu et al, 2015) and CO:COLLECTION_SUMMARY grown in RPMI or DMEM supplemented with 10% FBS, 1% glutamine, and 1% CO:COLLECTION_SUMMARY penicillin-streptomycin at 37°C, 5% CO2. Cell lines were confirmed via STR CO:COLLECTION_SUMMARY profiling and were mycoplasma free. A549 and A549shNRF2-BIND rescue cell lines CO:COLLECTION_SUMMARY were generated using the Piggybac system. Donor constructs were co-transfected CO:COLLECTION_SUMMARY with transposase (Ding et al, 2005) at a 4:1 ratio using Lipofetamine 3000 CO:COLLECTION_SUMMARY (Thermo) according to manufacturer’s protocol. 3-days post-transfection, CO:COLLECTION_SUMMARY puromycin selection (1µg/mL) was initiated and was performed for ~2 weeks prior CO:COLLECTION_SUMMARY to experimentation. A549shNRF2-BIND rescue cells represent polyclonal pools. CO:COLLECTION_SUMMARY Cell viability was measured using CellTiter-Glo® Luminescent Cell Viability CO:COLLECTION_SUMMARY Assay (Promega) using the Envision 2103 Plate Reader (Promega). Cell growth CO:COLLECTION_SUMMARY curves were generated using the IncuCyte System (Sartorius) according to the CO:COLLECTION_SUMMARY manufacturer’s protocol. Curve fits were performed in Prism. Clonogenic assays CO:COLLECTION_SUMMARY were performed by washing cells in PBS, staining with 0.5% crystal violet CO:COLLECTION_SUMMARY solution (Sigma HT90132) for 5 min, and washing 3x with PBS. Four replicates of CO:COLLECTION_SUMMARY 20-30 million A549shNRF2-BIND-luc, WT NRF2 or Neh4/5mut cells were treated with CO:COLLECTION_SUMMARY 2.5ng/mL dox for 48h. For A485 testing, cells were treated 1µM A-485 or DMSO CO:COLLECTION_SUMMARY for 24h before harvesting. After treatment, cells were trypsinized, washed 3x CO:COLLECTION_SUMMARY with ice cold PBS and flash frozen in liquid nitrogen for metabolic quenching. CO:SAMPLE_TYPE Cultured cells #TREATMENT TR:TREATMENT_SUMMARY No treatment was administered and this is a study based on genetic variants. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Cells were trypsinized, washed 3x with ice cold PBS and flash frozen in liquid SP:SAMPLEPREP_SUMMARY nitrogen for metabolic quenching. All metabolite extraction procedures were kept SP:SAMPLEPREP_SUMMARY on ice. Briefly, 350μL of cold methanol containing in-house metabolomics SP:SAMPLEPREP_SUMMARY Recovery IS (Stable Isotope Labeled Internal Standards) mixture and 200μL of SP:SAMPLEPREP_SUMMARY cold chloroform were added to each sample. Samples were vortexed for 10 seconds, SP:SAMPLEPREP_SUMMARY homogenized with beads for 2 minutes, and centrifuged at 4000 RPM for 5 minutes. SP:SAMPLEPREP_SUMMARY Next, supernatants were transferred and 200μl cold water was added to perform SP:SAMPLEPREP_SUMMARY liquid–liquid phase separation. Samples were mixed and centrifuged again. The SP:SAMPLEPREP_SUMMARY top layer aliquots were transferred, dried and reconstituted in 100μl SP:SAMPLEPREP_SUMMARY acetonitrile:water (8:2, v/v) containing the in-house metabolomics Global IS SP:SAMPLEPREP_SUMMARY (Stable Isotope Labeled Internal Standards) mixture and submitted for SP:SAMPLEPREP_SUMMARY metabolomics analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Shimadzu Nexera HPLC CH:COLUMN_NAME Waters ACQUITY UPLC BEH Amide (150 x 2.1mm × 1.7μm, 130Å) CH:SOLVENT_A 100% water; 10mM ammonium formate; 0.125% formic acid CH:SOLVENT_B 95% acetonitrile/5% water; 10mM ammonium formate; 0.125% formic acid CH:FLOW_GRADIENT 0-2min, 100% B; 7.7min, 70% B; 9.5min, 40%B; 10.25min, 30%B; 12.75min, 100%B; CH:FLOW_GRADIENT 16.75min, 100%B; CH:FLOW_RATE 0.4 mL/min CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive Plus Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE UNSPECIFIED MS:MS_COMMENTS Samples were run in polarity switching mode for both positive and negative MS:MS_COMMENTS acquisitions. The Q Exactive Plus Mass Spectrometer was operated with the MS:MS_COMMENTS following parameters: Sheath gas flow rate, 50 units; Aux gas flow rate, 13 MS:MS_COMMENTS units; Aux gas temperature, 425 °C; Capillary temperature, 263°C; Spray MS:MS_COMMENTS voltage, 3500V for pos and -2500V for neg; Scan mode, Full MS scan with MS:MS_COMMENTS data-dependent MS/MS acquisition. In Full MS scan, scan range is 60-900 m/z; MS:MS_COMMENTS resolution is 70,000; AGC target, 1×e^6; Maximum IT, 200 ms. In ddMS2 scan, top MS:MS_COMMENTS 5 ions are selected with an isolation window of 1.5 m/z; resolution is 17,500; MS:MS_COMMENTS AGC target, 5×e^4; Maximum IT, 20 ms. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Ion Count MS_METABOLITE_DATA_START Samples Sample01 Sample02 Sample03 Sample04 Sample05 Sample06 Sample07 Sample08 Sample09 Sample10 Sample11 Sample12 Sample17 Sample18 Sample19 Sample20 Sample21 Sample22 Sample23 Sample24 Sample25 Sample26 Sample27 Sample28 Sample33 Sample34 Sample35 Sample36 Sample41 Sample42 Sample43 Sample44 Factors Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox- | Sample Label:luc-1 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox- | Sample Label:luc-2 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox- | Sample Label:luc-3 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox- | Sample Label:luc-4 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox- | Sample Label:FL-1 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox- | Sample Label:FL-2 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox- | Sample Label:FL-3 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox- | Sample Label:FL-4 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox- | Sample Label:45-1 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox- | Sample Label:45-2 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox- | Sample Label:45-3 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox- | Sample Label:45-4 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+ | Sample Label:luc+1 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+ | Sample Label:luc+2 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+ | Sample Label:luc+3 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+ | Sample Label:luc+4 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox+ | Sample Label:FL+1 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox+ | Sample Label:FL+2 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox+ | Sample Label:FL+3 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Ful length NRF2 | Treatment:Dox+ | Sample Label:FL+4 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox+ | Sample Label:45+1 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox+ | Sample Label:45+2 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox+ | Sample Label:45+3 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-NRF2 Neh4,5 mut | Treatment:Dox+ | Sample Label:45+4 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+DMSO | Sample Label:DMSO+1 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+DMSO | Sample Label:DMSO+2 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+DMSO | Sample Label:DMSO+3 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+DMSO | Sample Label:DMSO+4 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+A485 | Sample Label:A485+1 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+A485 | Sample Label:A485+2 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+A485 | Sample Label:A485+3 Sample source:A549 human lung epithelial cells | Genotype:shNRF2-Luciferase | Treatment:Dox+A485 | Sample Label:A485+4 Glucose/Fructose/Mannose 44031387 53479315 46484686 58574631 57640605 60672724 29781287 30056911 36894381 33720989 43657061 28869306 18545198 27111886 31988833 33043181 16228864 10747087 14713395 13103419 25132685 21783023 35700668 24864968 39163835 35206708 41097839 42095764 40417576 24404406 22657426 26592082 Glucose 6-phosphate 1739085 1844351 1835353 1789145 1740893 1773397 1719837 1829425 2164712 2020423 1922853 2008270 1739322 1883310 2260474 2119228 1884419 1628580 1851387 1765006 2672681 2027913 2663123 2518082 2763694 2519499 2687239 2725618 3472143 2925999 2370889 3096179 Fructose 6-phosphate 3503474 3747467 3772339 4195038 3891257 3996503 3908288 4321118 4080507 3881864 4276998 4595452 3958972 3900051 4082523 3909839 4421466 4722536 3526406 3917099 4481399 4044575 4596172 5241304 6612654 6044691 6189052 6189801 9309788 7608536 6797266 8606274 Fructose 1,6-bisphosphate 381102 425961 385027 498672 263921 248860 422064 519019 274643 184268 290721 366243 261477 308010 377089 402549 956646 1052924 919301 951002 382340 452167 501801 617210 264211 192616 308376 247267 344366 368411 255812 579156 Glyceraldehyde 3-phosphate/Dihydroxyacetone phosphate 4106481 3116117 3679895 1262284 5313117 3151304 5044230 1970395 4814238 5533389 3066739 4384678 5127936 6030636 4742645 4889163 7241135 6504306 4310053 4254323 6131703 4740771 5841654 5104425 4275325 7119479 3647125 5209677 5482965 5492524 7120037 5703187 2-Phosphoglyceric acid/3-Phosphoglyceric acid 4216697 2901860 3100392 3173282 2956795 2840656 2461843 2346023 2730580 2712621 2744456 2482592 2977794 2883582 2637493 3181731 2660253 2656078 2361703 2513167 2561079 2676285 2440894 2521238 3055860 3289410 3288172 2918009 2667664 2627922 2585339 2705448 Phosphoenolpyruvic acid 850546 1287972 1850963 1548803 1538634 2382657 2375604 1853823 2621058 1552526 2073812 1919250 1868703 1813533 1808619 1618515 2361608 2291453 2107662 2061958 3222555 2712205 2703304 3222765 4616775 4744583 5149202 4119633 3007065 2885156 1741906 3667288 Pyruvic acid 27965709 23316163 25503187 28581735 24387119 23862279 31726766 28541156 27212425 20514427 21006392 22845421 19439602 21241784 21778296 19286925 27411086 33851221 28440237 24591284 24579850 20623014 22557188 22991612 26143576 34134015 24414526 27583021 23351780 23806342 21188874 21426110 Succinic acid/4-Hydroxy-2-Oxobutanoate 375540862 381008578 385982855 440155188 378897599 398993925 375592193 360551651 346912492 302641373 339376342 352109657 287862312 301331624 308886948 322926867 336557127 326089906 319728021 327562314 314641492 276039435 349386667 357190367 402273155 350373312 359145969 365962925 517445857 451439594 397009771 519072023 Alanine/Sarcosine 198563993 211356504 194912997 236392682 160699781 198169549 217237666 227583811 184123602 148732802 184149537 208830421 147746973 169124755 167836606 166626239 311464681 332871974 289391999 311437228 223355172 202083173 240276872 271163322 239146990 206768007 220483744 230675461 293523791 254901575 225994581 309839757 Arginine 1344133331 1445079617 1410106301 1539147460 1298185014 1433287868 1379485269 1372325870 1484118529 1326552661 1472583121 1535930218 1270746018 1298712088 1270289107 1339916969 1208546659 1150749148 1026589118 1132173752 1337864876 1201030070 1325085020 1436667051 1763169897 1660059816 1625912277 1620985635 1648719303 1477245678 1394416977 1468220368 Asparagine/Ureidopropionic acid 134133855 152432541 136513950 168870712 111251175 124422658 144846483 157254407 146737961 133823376 161004395 176133514 109574114 116149612 119466987 126532783 91877947 100389919 77158515 93663034 130070139 118676467 142330403 167740627 229791771 174107456 189213719 193889760 286467903 255977125 218571952 299688959 Aspartic acid 39791811 40542334 38746965 45366910 33936714 36822394 43483368 41069634 42510670 31933059 42007758 45273744 37108139 38782393 38971951 37166974 28871600 33029287 25687914 27331178 38897056 38074904 43152959 50351949 67936080 52524234 54747896 54777376 77193003 72337798 58834351 84976380 Glutamine 1264462647 1447050067 1281229063 1570360396 1195745158 1352993786 1517808569 1555498096 1355207539 1177005294 1385269305 1487948868 1273229020 1274591258 1326128960 1464480357 961575816 903893906 731366785 876898556 1332533023 1144127804 1395061966 1599343944 2028442911 1664471366 1734121777 1799355560 2258549272 2097732766 1781996741 2389110730 Glutamic acid 933644564 954854874 992403556 986848158 889417490 836962142 1052033754 953104917 921489032 847484949 996431549 1064726355 805711014 836123481 839198846 781870375 939567872 1107831812 926549572 881069845 787587713 781719795 869743678 976192521 1265696486 1011948463 1007388153 977728887 1789149696 1752332410 1416607936 1946186725 Glycine 11560643 12547846 13985958 16713984 11241334 11298811 13776012 15194502 16973225 12776917 13536051 17822191 22187395 21449670 21547493 22568468 17341845 18138499 16315884 18123941 21690815 17501143 22033429 24706316 26726258 21126966 22849426 26056914 33853766 26118504 25273476 28559344 Histidine 227070926 267234093 250792534 310257932 205643810 259239194 298583542 288732087 250008905 198795342 257740923 290769496 225412652 259815959 247817145 266222787 243663697 282754686 213047761 210480213 251451867 215109588 266006412 304976872 430558819 342860430 356584956 363973507 552774400 427785464 357604574 571118558 Isoleucine 202588672 201326602 225509984 232000544 192658724 218287726 232704440 224501198 185587309 167015790 191926385 189647999 188110862 189310014 204896799 208021334 176159176 202120312 159226538 152766235 170264783 154692138 193032588 206637256 310268669 258941467 287409105 290639396 357380524 305576817 284228525 357863962 Leucine 209624501 221758267 221155092 233790203 209082879 220863876 243426586 264396453 192325514 182533363 226040761 218083510 182881942 223903330 216756201 219988544 168469485 193951662 161717603 176623779 172956370 177718979 207187157 237058784 325181188 305278599 318329915 315010862 368444683 362772109 314489754 453474806 Lysine 79786289 87333143 87732887 96772279 74644743 95915572 85192542 83931722 84243480 83392256 94104443 99601204 77454599 93236396 91162038 95057969 92998297 106162092 91765665 88493465 93464242 88814055 106193227 110121452 113186568 120539312 116023208 125264900 103709704 110216638 112648157 132047139 Methionine 27001876 26721376 28279792 31065770 27693554 31307563 31293646 32190326 24583884 21429912 25975468 28103820 25010411 26445891 27818228 29721243 25071263 26582545 23047109 24283522 24773114 23191597 28091409 28847892 38436494 34736755 32819406 37460916 40941669 40125484 36213618 45520493 Proline 1032185096 1006650390 1004881232 1084408999 699881628 807542480 875842538 859120369 777869519 798614808 948400681 1003195979 593745070 651565396 715515387 650110477 727646386 788746053 689833113 684727789 733384060 716298285 840827506 903901832 1629771076 1311924245 1300550787 1311134540 1889832988 1902627487 1637382636 2090470660 Serine 18997233 17968511 19842415 20707986 16925724 17354248 18413387 17698445 19335050 15941440 18891106 19875753 19682344 19406339 20800729 22927701 18586434 16849225 15976849 16097322 19481590 16070871 22167053 25707542 27078267 23429538 24042784 25494693 27168985 22454073 19385789 26656510 Valine 819964360 778833177 880590648 890239284 664710519 711432446 794198777 741371472 680564406 657095220 720473553 692464659 678058090 669311293 706816508 725017795 834459545 908538186 49774340 50608798 47053177 42416571 55386212 56652013 71390261 59729584 60627663 66532897 75789028 68817505 64020002 87033029 Threonine/Homoserine 27961874 30828557 29804143 36157291 29027431 30356941 33383249 32956531 30834227 29266305 31692887 33929327 27800915 28143674 29980125 35523605 26878576 25948925 24298512 24945106 33727541 28825123 34503914 41977072 47418751 40906386 42274552 43438907 54340317 52619305 44714064 60867972 Tyrosine 5051114 5600342 5769484 6843011 5768705 5449872 6412500 6566055 4928002 5170808 5307864 5963213 5346234 5353318 6233046 6317992 5277909 5232367 5451156 5147278 4999847 4640696 5690569 5943578 7993938 6506182 7575739 7458689 7961899 8778125 7848359 8487873 Tryptophan 19862220 21767039 20157998 24166200 19296869 24300880 27723281 23300786 21226816 18383092 35494364 22095952 20206309 23208449 20622309 22459684 17868582 18420523 15781006 16838431 18509608 16953914 21742777 22605550 35452422 27334120 26257849 27048752 31416364 31601852 31791063 37085098 Kynurenine 211764 322091 162130 195305 239039 319208 207002 344856 185504 230981 283382 260604 192022 244816 198666 132084 84778 138113 97047 108889 129498 237420 161883 137584 442724 333339 373664 408057 481123 293512 325723 439346 Kynurenic acid 7432443 3158454 2477799 1775168 1046909 1216585 6467864 1852551 945488 930440 597621 882091 652046 750149 494761 415055 1110171 666791 562733 374263 747684 589950 439381 119020 708423 373257 99563 234696 1082089 373998 341901 400296 Niacinamide 1107361453 947250245 1015954324 881039893 1094145584 1061207738 1427665097 905005592 1234991246 1483909825 1129192958 1435562977 1253589169 1412032945 1552239799 1363662628 1363251468 1406957365 1518544685 1521865275 1337598372 1154487266 1664479862 1476696067 1909054108 1985433399 1628478795 1773584962 1695221001 1675126068 1503769926 1722832999 Phenylalanine 61982719 69100225 68383617 70382133 63601989 71116266 74839172 79523300 58264095 60429513 72848785 65501716 66899687 62503849 69483780 68508898 50290422 59165931 49082226 55900822 59471626 57307958 72812074 68252566 93401844 87077520 94333020 88035159 104910254 94549150 86137368 109655443 Indole 1900483 1759819 1538313 3757655 4595683 2281652 3635647 3436341 1974260 3565511 1838049 3365959 3864371 4030019 3028241 3875408 3761923 5813212 4074085 2824278 5001904 4990547 3863996 5255366 576047 4115770 3431673 4740931 2772032 2811403 1785945 3423338 Indole-3-lactic acid 427418 504433 655684 478372 513551 496193 449514 450076 344662 401106 304350 480542 630243 617950 548276 545927 296718 144583 264111 184570 424034 409001 541248 624118 692154 803923 539460 676072 941056 860546 759080 853627 Propionic acid/Lactaldehyde 32176460 32289251 32777762 37650775 32838409 34934114 31863765 30683044 29585998 25764388 28500092 29346341 24863508 25249091 25997978 27569853 28476007 27443873 27261768 28753603 26614054 23562976 30529470 30306725 34172325 28979600 29865355 30388816 44126057 37537375 33376810 43547292 2-Hydroxybutyric acid/3-Hydroxyisobutyric acid 10583087 10869954 10582441 11894818 10118048 10283642 10349905 9479340 10311236 9022203 10285675 9941552 11472342 10925157 10957634 11323876 10921703 10839824 10094943 10575409 9812262 10083165 10544826 11392659 16611974 12766266 12520104 13619569 19241399 16911552 14384831 18396153 Caproic acid/Isocaproic acid 227859 359642 450967 323168 338342 403341 351360 477152 443461 189360 223443 170904 228965 339499 139399 143814 140322 278768 345807 184967 331427 219253 279906 251907 118295 63876 159351 192543 122132 108396 n.a. 335543 Glycocholic acid 189037 352399 298972 559766 406952 414572 359906 487145 384120 490739 296449 330628 429427 622320 418212 451765 451603 391114 353870 325109 380136 249741 428856 306366 303058 398253 468601 364316 715610 763874 662782 637060 Taurodeoxycholic acid/Taurochenodeoxycholic acid 59989 75826 62274 47408 117438 64975 86784 n.a. 86773 95330 70057 79170 60372 53994 71885 45608 24795 31036 n.a. 31172 66897 18224 74706 29923 128867 84921 69567 86178 56017 75991 56104 109380 Cortol 1402308 1019822 1061655 1820519 1855018 939803 2311444 1724016 1261573 2148812 1241915 2249113 2629701 2322843 1772106 3282812 2334000 3516690 2686171 2186559 3507830 3559200 3233776 3459985 n.a. 478872 309123 1073321 158576 77230 n.a. 473292 Cortisol 462152 93161 96843 585453 411470 121089 725510 541310 572009 756037 303576 715622 887088 668378 465422 1140853 862296 1478419 1087355 949800 1247737 1347970 1208069 1184647 32200 713330 1388158 1386942 2493865 2187393 n.a. 930840 Adenine 87989436 92611461 86184045 71158497 64951668 78480703 86547139 69658518 61584456 54295954 80181867 75747160 70859189 62098587 75612801 83151750 64385558 68187437 62151563 66951366 83193252 84166100 76569716 108024218 93368736 75836475 76522881 78734049 68212730 58935271 66508032 89778460 Uracil 8289409 8514803 8702162 8599956 8130519 8393638 7503128 7921004 8630676 7918686 8265685 7681841 7355948 7968390 7364147 7909511 7405911 7545477 7425762 7098893 7324031 7340133 8298646 7246052 9213857 8436438 7624155 8908264 8436325 7748232 7229758 7408891 Xanthine 28310851 31407118 29475432 32120141 29716398 30268921 29171709 29771348 32390192 25940772 25522395 27821354 27808910 27214327 28352059 29095942 28647710 23272763 21983966 23336855 32666808 24208919 32996988 33993325 46318369 44755977 41939935 42375116 48406562 39143935 30889616 43905979 Adenosine/Deoxyguanosine 237774460 222230527 209105522 237368503 193189444 211266987 200420939 205818696 206547628 172665320 202668926 200105248 193465942 205036473 204969415 195075471 179235650 182210233 171554170 168161627 186105326 167261703 217097570 223991736 377488101 316440743 291144919 287884527 353871030 322392746 255199644 371861150 Uridine 3122703 3264276 2957029 4735566 3216301 3557363 4228202 4020202 2761031 3160211 2880341 3872389 4413349 5088539 5734318 5198271 2435560 3308691 2657546 3011742 2554323 2307645 3442772 3268594 5610317 3792532 4197184 4093198 5030216 4540044 4569860 5925124 Cytidine 6702908 7908492 6018569 8958027 7817978 8377180 6713509 10083435 8834067 7373236 6981748 6207772 8178726 8264914 11079195 9470086 11821698 13273132 10358794 12129911 7881085 7469416 8671798 11546095 9658052 10437322 9891294 9649506 14734040 13164150 11644048 13716266 Thymidine 2137073 2034678 1901796 2132476 2668518 2534819 2650470 2680107 2248366 1731018 1916561 2080282 1333372 1484047 1351713 1558055 3010538 2946666 2409261 2531708 2085574 1874254 2152326 2321308 2966169 2375537 2197625 2144324 4033540 3560548 3034083 3959534 Inosine 1018915 1196683 920057 1785980 1029798 1381969 839006 1983652 1158636 540815 1048695 1182743 1346920 1612841 1208429 1641116 509246 451570 332713 554911 727056 582029 788763 927940 2495238 1614336 1892001 1685563 1841530 1494259 1026556 1806868 Xanthosine 3491361 3966535 2643427 4006979 4187271 4323774 3452560 4800065 3852546 3391798 3290889 4508571 4469653 4614340 5606596 4989477 6410828 7766764 8182972 8823867 4418516 4154060 5008213 5820699 4205981 5125130 5215539 5285200 8019228 7748249 8551648 10087624 Deoxyuridine 289243 237085 273473 365973 289728 266412 315751 281117 329815 241320 267188 360533 107199 129468 n.a. 147580 425324 283902 283049 447074 368371 291271 375512 412338 637281 425728 413588 562258 894639 722899 629373 996747 AMP 948936 1320752 1206798 1548189 1547024 1438681 1607847 1428990 2027212 1744571 1761945 1746891 1793531 1797541 1906526 1869629 1805035 1539052 1953459 1926542 2009085 1841543 2527096 2279577 2224123 2626398 2359773 2506339 2318717 2183149 2485880 2580419 GMP 88900 n.a. n.a. n.a. 57203 19372 45978 62775 36773 62440 98365 70879 53758 150734 64476 147679 134435 121973 85196 85628 129832 80870 94510 95413 80280 79266 159115 147309 136230 112661 232494 129561 UMP 584144 634651 533489 733660 704098 775974 605774 683799 816626 796483 869019 823398 865972 848240 949578 901155 820355 690973 798619 596230 830189 690869 666482 681589 874829 779295 702996 881620 848111 892592 958710 836091 CMP 13262 19740 34360 31170 75673 157704 72423 98693 89610 70784 132589 63892 92796 126852 119018 174397 126044 125813 129486 164690 104408 139357 129148 217670 134301 188230 104098 260328 255935 252687 208413 182926 IMP 487485 633168 410673 561039 483404 438857 372596 581332 579800 437768 454888 514803 568502 574412 528746 700985 313330 256293 272960 280745 481968 375423 381108 582321 625328 804185 764742 904388 827259 810294 574279 898491 Arabitol 2140129 2360874 2194670 2588579 2006297 2143097 2370141 2673537 2066157 1837858 1455607 2195964 1812669 1905113 2063881 2052968 3823429 4199577 3770567 3551383 2380884 2384243 2718432 3125191 2867349 2454732 2721427 3005477 3878196 3566154 3238995 4601269 Sorbitol/Mannitol 52706219 51999744 44886653 48905317 48182879 46322984 64485391 65969999 50307861 45902480 53025680 57620641 47891572 47176731 44820566 46000655 60300119 50801559 49485896 53674370 53586485 46338840 49023281 54753840 86107463 53484461 47369467 45144199 66275904 65946807 55568616 67001682 Erythrose 4632676 6432717 4610180 5697011 5694098 5850290 3092958 3907416 4019609 3533142 4329286 3149042 2167173 2812802 3590015 3664150 1821205 1583766 1945846 1709473 2701989 2110102 4208893 3071732 4046101 4147583 5818210 5022892 4190768 3298170 2595853 3011278 Ribose 1143123 1294470 1189044 1341817 1244214 1390839 1037521 1024473 1002938 850183 1123592 921385 675012 914431 960952 954411 918934 849217 877636 736821 867232 720749 1053285 796544 1222234 1342658 1234998 1304295 1369736 1042587 1071411 1272055 Sedoheptulose 82392 105573 132518 123463 71314 116056 76623 87004 166191 91245 96076 60905 n.a. 58247 45405 24143 68543 103575 64530 14783 82754 17106 74153 75042 209095 98963 110502 16634 98145 106705 76927 149538 Fucose 1-phosphate 2796306 2353444 2683136 2503045 2235944 2535227 2787046 2169712 3169927 2981019 3456082 3470879 2251495 2481660 2662105 1986345 3053783 3531405 3115718 3207088 3344885 3546537 4044005 3998764 3281665 3548777 3488437 3181685 3662491 3667747 3481461 4161444 Ribose 5-phosphate 1008696 788276 874637 825518 1068357 886064 1056003 917075 1031504 1299293 1156636 1378449 1206634 1280618 1466782 1414500 1164494 1311312 1223651 1157195 1356693 1211007 1608883 1598894 1469245 1772487 1535312 1654562 1646034 1388800 1515690 1686079 Sedoheptulose 7-phosphate 122103 142366 97193 145036 95089 180560 153062 106734 128455 99612 194434 154153 117139 134849 176531 130675 81042 n.a. n.a. 97600 94998 64943 125266 148768 282299 227102 254692 191477 231409 161579 186043 219278 Glucosamine 6-phosphate 360434 353307 338292 373648 284082 305429 306341 263989 324391 285587 349568 328470 300582 293151 293279 265988 403841 450791 380514 359510 427606 339746 470169 456733 665373 614659 554443 542065 917038 839685 658126 955368 N-Acetyl-glucosamine 6-phosphate 2028918 2368268 2187643 2557138 1989834 2204309 2396149 2516841 2552681 2208038 2413953 2810479 1619320 1788639 1840281 1958073 2481386 2705366 2206058 2346911 2105262 1836855 2303821 2533290 3214635 2839135 2662583 2803899 4804050 4411777 4152949 5162189 GDP-L-fucose 180742 181934 120689 129993 121285 153343 150306 176924 194883 103297 187089 115506 121360 116951 164805 112267 206810 243364 187170 228746 147511 121402 134659 153096 204508 203429 176206 253172 185579 207701 147208 186830 GDP-mannose 222429 222423 229657 185352 180744 185866 206887 200278 209119 213954 236119 226093 142087 183292 203844 215909 189384 227627 132077 185074 188084 194626 246133 229699 269358 206740 217718 227448 366907 224084 238622 273030 UDP-glucose/UDP-galactose 45739 144736 143665 232932 125142 148871 192650 70039 196757 163992 217306 240743 201463 149253 203958 169770 204419 149928 181218 170011 228953 204424 177494 217053 213309 186208 242987 188871 192187 263830 182411 247945 UDP-N-acetylglucosamine 30890593 34445432 34914980 38046668 28870586 31062362 34658703 31612035 33290403 28664691 41258381 43090626 17607531 19781852 18888042 17643699 64382183 76092143 47508891 52685687 37979190 31487027 41735477 48291549 85906680 82675120 65165185 68975247 175508293 162570868 145941973 195705372 Urea 18772386 21113378 20053347 20630383 19375651 21018780 18297963 15869270 21720095 16885444 20575933 18113640 14318929 17801369 16672618 17586063 17773080 16436317 14005953 15391976 20759126 16782604 20189945 20073201 24948411 24936863 21399125 25093749 26645627 24439362 19584419 24127740 Orotic acid 523148 425332 400355 468357 393930 476297 314214 352489 555279 348196 520080 478746 341004 301198 292747 284532 377483 447973 340743 406793 754813 1000424 579312 797389 885621 663157 715901 348548 1849598 2648410 1372600 2748211 Ornithine 1210790 1241120 1158979 1337687 1023623 1014233 1001169 1220233 1174572 1021088 1213168 1150855 977129 49799 1061732 1089754 925627 729225 716361 655452 1050383 964344 1169819 1562185 1837535 1738321 1868796 1797076 2148731 1848721 1872674 1878043 Creatine 1282123372 1365898961 1306978362 1443923494 1089488280 1232798733 1321463523 1367795468 1294936300 1155171269 1297676547 1457688603 1125399176 1240748864 1314560243 1278771427 1219358228 1303322706 1160999542 1266329298 1303756192 1191121050 1478299943 1517621979 1527580994 1349076834 1389424412 1469617023 1953863566 1737219805 1580983380 2004464204 Creatinine 755912 552671 384335 367976 394046 495879 568659 596027 265170 389548 480699 396300 442981 493781 464021 507716 277712 299825 328084 369873 417254 436239 373529 352006 346510 439696 649802 369359 800814 798656 550138 521491 Citrulline 9610823 10457028 10265699 12799510 7810370 8547258 10257839 10614357 9526644 8429175 11748624 13020783 7125921 7257378 7280832 7368645 6882614 7744932 5669605 6419403 7516919 6926932 9346966 10523359 21368313 16944882 16994684 16316912 36494990 31976194 23569226 39173308 Putrescine 43914742 40515776 35671845 37505258 33862502 31000990 23379391 38622291 36029954 49484289 44541048 43555835 23671637 26010748 33833134 32033362 29169914 25810862 23956315 34236861 32709770 35285815 33055014 35552128 22403296 24661417 24365176 28606919 29002679 24868565 38182002 26610191 Butylamine 321823593 322405302 320602354 318378246 317199297 317789005 321585048 294506052 319770002 295299083 320385801 315976005 324674006 321104226 332215724 303968758 336818889 345063288 354232040 321022806 328311061 334463918 351152424 357352235 342275485 358326349 366576325 342494828 377172472 364989235 305574897 384787056 Trimethylamine N-oxide 4317418 4651319 2130891 4644155 4061112 4977034 3201892 7027216 4533266 4179284 3964975 5175161 4336676 5207180 6727233 5144994 5677510 3748413 4829019 4078887 5502481 5331359 4874161 5609480 6418832 4592595 4701946 6235672 4215987 3336587 4399870 2560459 Dopamine 24963503 26654895 26225156 25423715 26581522 23820436 26868401 25906162 25811908 25938078 26897921 28386920 29552470 29264003 27558275 28636523 28469508 30212091 28407036 29514520 28516504 27474188 28754325 29065750 30528992 27351779 27428588 27482396 29807698 29309609 29546852 30704387 Ala-Ala 259842 292071 289338 374803 205806 293254 187825 250510 269902 233497 244170 189855 151264 260714 280824 193549 288963 320648 112638 229030 256415 243925 280950 344569 453669 409143 258693 402216 590651 488389 346376 409936 Ala-His 2145430 2542471 2049142 2477685 1447506 2180935 1942821 1867586 2354368 1545126 2155247 2607837 1865255 2200308 1879782 2229542 2738458 2670159 1933218 2195460 2386378 2033038 2591667 2939033 3804554 2842090 2927124 2941817 3915886 3110716 2283888 3876250 Ala-Pro 1095266 1214236 1179515 1094584 1093881 1394673 893886 895124 1222019 856145 1125609 893085 855988 885080 727269 577565 1128723 1030543 825465 796633 1290298 1283537 1252454 1388429 1761993 1797150 1659754 1823461 1732483 1533686 1341993 1943965 Ala-Val/N(2)-Acetyllysine 39112813 41892195 41042370 38165797 37921726 40212660 38837468 36015234 42140308 36766807 39841354 43019000 35002544 39870503 36394940 34236168 42363848 43309542 36314544 38250504 40902562 37752250 41209273 45140270 50622356 50418931 49567585 53759477 58828054 55663679 45606888 61220600 Arg-Glu 1178746 1369280 1299398 1569022 975394 1100538 1000501 1074878 1438388 922099 1347801 1235501 967438 971910 983023 1035826 1950779 1766268 1430314 1478909 1634116 1350810 1606878 1926414 2513053 1884240 2016009 1964913 2374597 1486879 1109757 2176188 Arg-Leu 11645431 12091102 14640256 13619848 10728039 14500866 9319975 8815589 11326804 8897435 13009936 10277116 7641978 7792548 8729199 7865244 8981092 8371063 8592002 8452395 9895781 9771697 12680534 12032366 10980040 9723848 11735515 7869702 9538390 7449291 7360607 11210972 Arg-Pro 2029037 2273546 2424053 3074670 1589203 2749746 1752296 2511394 1933772 1313712 2339574 1837309 1216903 1422495 1607169 1249540 1669295 1506714 1294832 1308094 1376253 1498163 1537632 1766374 2491563 1753059 1950787 1654609 1954050 1979191 1543486 1853896 Asp-Leu/gamma-Glutamylvaline 2066399 2303675 2276288 2544714 1210832 1472804 1476881 1692333 1957488 1801557 1980174 2590310 874038 1269803 1073628 1199072 1455049 1292655 1318625 1174842 1663830 1343792 1797187 2162831 4219033 3406439 3647491 4096563 3673407 2847832 2627031 3554690 Asp-Lys 2530436 2923821 3851723 4144078 3018761 4855281 2702576 2661394 2979353 2201505 3741731 2761184 2084864 2224907 2394967 2060024 2879092 2662845 2498330 2194187 2946520 2963724 3951844 3691342 5033540 3528500 3777206 3592148 3766243 1999418 1962812 3513156 Glu-Cys 846043 1112938 955586 777139 750118 636488 1270258 677803 1111888 715646 1403138 1135994 n.a. 180277 n.a. 87127 9398257 10215654 10133312 8673158 1894675 2183898 2388280 1836909 2265722 1988612 2060448 2081364 1591560 1636089 1108714 1380254 Glu-Gln 16670137 18334504 15836291 20668548 12471925 14012598 15496088 18442700 20242304 14612189 17032455 20135163 19089766 19418227 17799454 22555137 23004987 22005114 16778837 20130159 25233633 19491846 23779651 28328453 29153062 22625060 25612701 27637610 31489541 23454513 19594599 27342472 Glu-Glu 3437420 3179635 3897592 3582936 2327676 2793161 3513034 3491224 4066927 3008848 3862217 4522939 3230713 3449807 3642192 3224477 6219670 6403111 5791342 5115394 4234816 3826177 4423053 5604351 6523233 4476416 4975042 4584614 7898876 6597975 5139227 7126360 Glu-Gly 156474 89449 163271 n.a. 36736 90902 226757 127163 197210 33169 171225 213600 170071 136390 211778 212267 407243 310547 340326 221076 228784 304627 191965 338781 507203 263606 301104 332459 434931 213874 242880 384604 Glu-Leu 117007 147730 146428 194849 139591 106864 155091 169686 136231 93382 208444 114331 58129 126396 65690 96746 611821 623973 493137 475405 240986 217523 188743 276476 281642 285672 257941 278321 234391 189466 138354 212302 Glu-Lys 3512582 3942540 4917268 5485658 3405808 6593091 2797692 3047182 4194711 2651513 4332255 3408376 2099137 2931511 2998158 2803783 2685412 2731165 2091307 2335952 3475468 3275301 5187715 4467267 5868840 4404668 4261602 4127844 4278890 2649752 2199753 4071318 Glu-Thr 57208 199883 152389 190730 116326 106234 n.a. 121394 70173 134720 78831 139629 52917 139577 94507 50031 931046 999179 821154 802780 227754 254391 211942 298588 235483 166666 127339 195005 146483 301739 116598 114699 Gly-Lys 879580 746322 1037080 985767 823995 1255350 650987 674529 781646 625455 1152346 828515 487138 682377 720888 614426 673156 739632 549889 677867 922954 813063 1322052 1022901 1145677 1324088 1126092 1259233 1065753 873624 671123 1067153 Gly-Val 3396949 3613335 3686836 3163640 3814749 3306634 3613740 3742747 3459285 3836852 4486878 3479009 4218922 4062421 4082325 4246544 3392580 3468958 3777740 4215130 4082823 3937073 4436111 4050087 4196507 4380007 4721733 4379255 3974613 4003804 4007116 3944614 Leu-His 458948 595226 476383 659661 457015 529120 525901 530209 543125 529639 744556 637278 348675 508249 432200 491736 1003186 1442687 868963 835352 806735 581685 845052 970723 1096111 1064340 896454 964554 1409544 1805715 1070890 1687280 Lys-Gln 210584 262409 296063 425807 304679 452372 186339 307364 210630 173030 361015 258352 171699 163801 226584 166600 370708 522722 417337 405936 197051 335255 436348 398383 694859 356141 408337 367105 477539 206504 149626 439247 Lys-Leu 6241742 7474600 6265426 6411485 4122496 6650572 4247152 5699586 8476679 6642032 7806624 7157503 5974889 6984849 6689657 7266582 7240839 6135411 5845489 6752253 9237566 6736181 8308993 8060876 8257802 6841171 7474368 7651839 6605049 5055344 5450629 7193062 Lys-Lys 278650 438331 613953 520158 561467 935163 490912 353797 386772 327011 798373 563405 182430 249203 344277 384754 651840 824614 508696 650375 832084 811287 1049086 1030474 1616566 1124186 1572014 1281868 1604224 796033 711619 1510890 Ser-Gln 123507 158220 45506 41400 64041 194084 179112 149901 142415 190832 214216 241378 47209 130501 41203 223680 238314 168349 155962 212508 217626 176462 262300 243778 387212 356741 159664 208995 226699 125141 99562 307129 Ser-Val 375414 313580 398152 249579 419666 305387 592848 415566 51079 22594 315183 263166 116221 99815 123596 57564 25459 214000 76488 216323 81910 69506 398900 422958 448639 293190 271173 575972 821412 1065841 704529 1276578 sn-Glycero-3-phosphate 531823 252961 260347 289666 426768 495304 505436 435913 525241 389957 407175 374958 371845 616034 551041 590509 947544 1073714 699267 730187 496299 564300 586663 561066 624268 634228 398726 529298 717864 593923 785930 763443 Carnitine 1684201389 1720567501 1649918949 1737486356 1502801328 1648108850 1881308096 1785647294 1263927710 1220704294 1420087219 1444509063 1488236448 1555828680 1714056201 1533860654 1665775709 1783140271 1633275076 1547010683 1278416798 1291778520 1403229854 1516225359 1623396903 1338751218 1410955926 1415561149 1676470992 1686399482 1389674279 1832191586 CAR 2:0 195362688 205421963 219685182 217258700 241286648 229886957 255333944 244601312 188616699 174706712 177464723 197955499 195399580 194868234 191883920 191661149 320130780 346898564 335811144 350123455 222566562 199924676 232996969 260930034 324467635 270094648 257808193 289783011 472388375 368566747 301808244 407382486 CAR 3:0 303000892 345743564 341130984 358374475 268380069 296089621 336053644 293913897 274733557 244422266 312673930 299663512 380021091 381410004 374292219 357251906 282260858 371465297 303841575 272233024 266716382 277287685 321511628 309645028 432776708 378696732 386392105 354999118 595016649 543053485 476491734 626119386 CAR 4:0 65492385 62424279 67203539 69886529 67568450 68903976 81048952 69766613 61121048 59313777 66178887 74313887 63005277 68260778 66996317 61994938 67147568 71278001 64483603 55677150 63122648 62615232 70243433 83023410 89727849 79515536 75624950 73266005 93365391 101859189 83310530 102028527 CAR 6:0 1643919 1457517 1858951 1659755 1730509 1910495 1984172 2371768 1508999 1452962 1795302 1838829 1320714 1739085 1591147 1411579 1901896 1649414 1443675 1496266 1274578 1451547 1595566 1930014 2237965 1951805 1729112 1909275 2276656 2298287 1866934 2785072 CAR 14:1 229561 306685 334637 680533 358131 308107 1172578 875825 403630 597057 568060 453395 1255360 1688318 1297660 1039571 684841 716792 769342 320830 460946 251426 403220 533185 287989 1199331 925878 1178946 301299 698737 354601 311047 Azelaic acid 2939945 5087435 5279466 5979386 5577575 5566797 6528857 5384281 5351396 3096678 3453780 4779666 3090062 4071187 2764960 4334183 3231155 4574824 4480174 3140309 4457479 4106179 3569480 3895940 2492721 3203993 3858484 4621933 2990914 2941298 2416992 4495532 Octadecanedioic acid 114347 423788 611803 475827 590626 488091 405507 357250 459930 60493 40333 103623 65046 133287 110036 177013 78500 179117 119406 105744 248950 126982 187173 135787 122639 175720 123718 146758 172593 158404 147334 372421 2-Hydroxymyristic acid 28182 40154 42083 79859 46355 54415 70976 25615 22054 36290 55059 64583 29153 30872 61049 57510 108345 79769 50412 30573 37035 20407 43510 75431 48118 26300 24438 52901 58721 51584 54100 112072 2-Hydroxypalmitic acid 162975 280281 313374 311541 266400 249853 362695 275350 225289 148646 210093 217992 214045 130716 164713 175031 213639 169945 271511 188204 153549 125623 227924 214870 258912 185659 171968 164849 302579 309411 283324 497812 Phosphatidylinositol 1105446 1231262 830696 1493252 1786169 985968 1116840 1315256 1130843 1566046 739237 1387973 1340222 1430568 1602159 1480023 1427439 1770732 1959244 1545682 1336724 988819 1190036 1062904 737364 994078 1094515 1000731 1462608 1523898 1156445 1198917 CDP-Ethanolamine 933676 968512 815981 1050464 970060 912341 987610 1113877 952880 801291 981103 1024532 1009483 1093618 892849 1170070 1279807 1287241 1200395 1367104 1068582 990511 1038587 1295149 1534110 1149018 1338358 1301262 1114477 1002704 942882 1282938 Phosphonoacetaldehyde 9152516 10616665 10892964 11276629 10528561 11050308 12812828 11930474 8450735 7257712 9394341 8342144 9382127 9972920 9268718 9152663 7593138 8268474 6811644 6325632 7705573 7907741 8508684 9386135 13402979 10417627 11277792 11505762 14995920 13292112 10952469 15662082 Phosphoethanolamine/Phosphocolamine 530523 670060 606089 525496 449779 563063 615034 605212 1410137 1152535 1123545 1292463 1166570 872682 909867 1061395 1999530 2005419 1753497 2206997 2336163 2426817 2680398 3191420 1929663 1310123 1008350 1206896 2069479 1514644 1135745 1752077 Choline 2789830884 3021269063 2711949746 2932709369 2872154081 2828273240 3488438221 3108221485 3241832087 2965473813 2604582335 3122846256 3556031313 3508060488 3717080016 3724272045 4131568373 3940531131 3836302815 4256302106 3378986602 2733515192 3691665285 3651833178 3548424435 2932260391 2983405734 3161762284 4124838308 3185486679 2668005889 3401056424 CDP-choline 382447 431640 406074 476705 427889 301628 600083 424015 563007 531942 551102 651358 695405 737252 830769 748157 966050 1179366 1114405 1027026 829902 808182 975145 829967 774842 767105 725621 639704 767906 775903 555900 685670 Acetylcholine 201199477 219152421 188528060 220773202 162324559 174430525 210119136 211510289 166319829 160491074 170078663 180559144 184008380 189724877 213850803 191564575 205345101 203630466 182838634 186380172 172213851 166137660 185899904 202207369 199564567 176063726 181471581 189173444 250133665 226133179 190481499 246770802 Choline phosphate 3554121771 3966456905 4288743860 4240002991 4100969021 4273420003 4937338519 4390166581 3328255317 2712526415 3521701742 3328624878 3560480321 3679162388 3606456564 3559143847 2833212835 3054915360 2704466089 2408366474 2937244042 2932278269 3291554278 3539648502 4985386154 4056312795 4427351058 4342944622 5883076446 4878562777 4198783417 5721751470 Phosphatidylcholine/Lecithin 4676643 6487691 4751586 13877132 10540212 5786485 3573463 7619182 8628926 8092636 4952900 6034229 6124566 10859912 6092015 4568480 5714844 5880331 4246423 3655194 6695759 1130526 8543054 2983722 2062176 5210010 5089330 4174329 11256637 7430192 3280572 3167191 sn-Glycero-3-phosphocholine 161929570 195423106 163825652 172485480 163597634 199870266 240763034 176984042 203026123 170103279 200307579 209892574 157106021 171800245 166568766 182360706 475816998 512581973 428213882 382634003 246014853 230808150 302035058 275538735 319976495 285413432 263097050 289319444 582079964 636355953 466680592 585999535 LPC 18:1/0:0 2776796 4057758 2598216 6026563 4534922 4230575 3930471 4916484 5771572 5737928 4389818 4761134 3964757 4873993 4232319 3836767 4440928 3362421 3868510 3992654 5109464 4002060 6461950 4418679 4545899 6102719 6371669 7204187 10069328 10396613 8456751 9938164 LPC 18:2 199936 n.a. 126758 318825 345534 99917 55011 154849 78188 231352 149528 226820 196199 319141 211325 107163 200186 263508 343058 177653 353021 220009 424503 301420 168255 480928 621010 342574 302036 686030 651297 644422 Thiamine 2729614 3371309 2958883 3317536 3697027 3509498 2897234 3191870 3152469 2872972 2755399 2644207 2865019 2704690 2972051 2926566 2487743 1945666 1863318 1930144 2695024 2807995 3301117 3536944 3845307 3934708 3690980 3486139 3308684 2395204 2111417 2907810 Pantothenic acid 108591105 100493989 110788020 112433628 132783666 129775519 147724729 131721381 102728073 89218169 96729391 105322593 76376337 75087927 74046887 75622990 131804956 142189090 118423902 119935064 113494499 102387247 113356366 131564055 134161524 112441028 109021858 109921469 198646542 182568957 154311784 198783302 Biotin 10429117 8783781 10215750 9302317 11853859 9940724 11349065 9372733 9729123 7053213 8451267 8557805 7455436 9204477 7690477 9050081 9623364 8403907 9193283 9804848 9556326 9469599 7702412 8698813 8575665 8671875 8313456 9426731 10243420 11782219 9114150 9355143 4a-Carbinolamine tetrahydrobiopterin 182188 152084 189865 172329 184182 123925 140781 128398 175289 141189 170251 114963 143342 173686 178609 154501 85569 167027 83419 154937 174105 133199 188470 164012 200061 252915 152158 200355 196078 160160 155335 227243 Sapropterin 1969069 2244251 2511174 3091521 2445522 2938309 3336913 2629949 1915193 1360815 1928335 1814013 2571225 2865398 2583939 2870234 1258072 1463854 953934 818442 1403170 1708934 2005829 2152847 3022894 2292192 3192534 3069796 4515669 3624387 3477202 4156328 Nicotinamide riboside n.a. 329922 348561 412031 447606 284594 306983 503023 279643 282376 343198 354800 102112 74099 135679 81577 837963 967483 658266 809878 372008 379924 426323 460537 426927 355989 486070 373473 388439 356851 237099 390504 N-Methylnicotinamide 335988135 343905096 356438683 352062712 340101406 351763484 388556504 332352232 313285663 292440240 332133927 329515151 379740670 362551049 440328887 394793638 393881749 409881967 400365670 382687144 331677977 326731992 413471912 404260550 420714057 384034309 339590382 403128220 410768476 370563945 309027687 415832405 NAD 6792086 7895397 7121714 8275434 7518363 7095562 7260560 8317628 7523388 5853302 8134058 7396506 6544897 6366749 5758879 6124329 14336310 15068344 13479931 11811912 8211163 8659634 8347178 8992667 9223837 7180296 8522037 8260349 11790517 9784330 9007979 11998382 NADP n.a. 62006 218807 108723 257914 311550 185170 445313 402211 552610 417105 517073 331379 362977 398512 337355 1523240 1461156 1270622 1356751 1005810 918259 1023679 1303726 1056905 1086128 1156276 1075341 1193595 1074438 795738 1331226 1-Methyladenosine 1724300 1979278 1760397 2603905 1612843 1764316 1955020 1955499 1949026 1948588 1621227 2071221 1528428 1930095 1584590 2178657 1881030 1638999 1507657 2271300 1724860 1692743 2252945 2099731 2460429 2338842 2017076 2664721 4399688 3361785 3413352 3771693 1-Methylguanosine 312400 63943 172031 654131 1388522 150243 1436913 849470 663726 1155287 173685 800255 953122 1378191 645079 1444318 949729 1853687 1204967 1288758 1824155 1329702 924773 1418135 n.a. n.a. 54999 247570 68026 180810 n.a. 60249 1-Methylxanthine 1985977 1923597 2182060 2051459 1522441 1567579 1910900 1848522 1422640 1255784 1478487 1657604 1298072 1406488 1678862 1421438 3034613 2844683 3435594 3138072 1790572 1639535 2002771 2062718 1934700 1327647 1722462 1589263 2204024 2058878 1457837 2264772 Dimethylxanthine 44228023 53545256 95719712 58624456 57808046 62336632 29865697 30151133 36938557 33745315 43755583 29774453 19469510 27376465 31908176 33072423 16497537 10775781 14717875 12974988 25448709 22490054 35726890 24819622 39234809 35300532 41711027 41998363 40349540 23761732 22012254 26588932 Dimethyluric acid 1447833 1149389 1202967 1313450 1537690 1442489 1322112 1343441 1218983 1354295 1670174 1833574 1611377 1414196 1713149 1536341 1374341 1613703 1384626 1551022 1631462 1785671 1501848 2028017 1936710 1544131 1546362 1330317 1639985 1428129 1781463 1738135 Betaine 819964360 778833177 880590648 890239284 664710519 711432446 794198777 741371472 680564406 657095220 720473553 692464659 678058090 669311293 706816508 725017795 834459545 908538186 695459293 775840780 694385121 641326603 783312533 839058024 930674135 786859151 863016414 886726064 1091190615 961270578 824886136 1139369751 Cystine 376900 268055 293639 287381 199064 277409 193623 299618 370995 292958 317599 275785 233796 207034 277962 286266 240189 272074 148536 212060 182171 165170 197144 323000 384616 177651 323676 227439 267572 211733 119590 264884 Cystathionine 1223359 1399772 1367796 1600779 1289837 1362761 1544229 1504933 1126542 894995 1205670 1263703 1724607 2023647 1751392 2032995 569530 534190 462370 517637 1051206 908655 1047581 1224313 1951790 1540305 1678666 1626581 2561740 2466474 1967481 2722455 Taurine 74229830 74904875 68174698 76619357 58779539 60087193 78900059 81339219 50065979 46548265 62400015 62283296 53746056 57172527 55654888 56457742 124035733 126565373 129280249 117888712 70495269 67794976 80083958 85042965 63089538 51276399 56167964 52122580 77530133 68034416 57734508 75777600 N(5)-Ethyl-glutamine 1806690 2283914 1896682 2644433 2035345 2077308 1993692 2549245 2439346 2113550 2358090 2623062 1735409 1811438 1862598 2036485 1403624 1403338 1020913 1320766 1846626 1787155 2066317 2613438 3976371 3552187 3358802 3955438 5365593 4877210 4327692 5335962 Glutathione 489107523 519864242 547253349 544774170 480031481 524902391 543487893 508686068 551405077 507706292 575245660 569835698 171042678 171811852 168549608 151418871 1437033087 1489779441 1495103826 1423966375 727307185 714911405 766394500 745112830 730337929 701290565 640425407 651831548 577376807 514089617 520422218 597335923 Homoarginine 11430882 13643249 13636163 14856091 10657834 13155418 10917929 13223015 13869382 10917056 13164892 13430734 8544483 10422665 11196453 10608874 10514466 9862374 8702433 10119668 8123614 7194844 11403770 13005244 17340597 16556514 13449302 15805457 15872507 9614199 9535881 9394738 Methionine sulfoxide/Targinine 4662865 5061349 5069839 5807393 4715501 5418487 6272358 5812815 4345525 3935498 5041761 5290144 3602840 3982421 4264145 4304963 3094415 3297116 2441749 3297980 4124158 3372980 4151137 5094134 12876012 11249909 10076108 10682564 14407242 13895645 11801153 15537527 2-amino-3-methanesulfinylpropanoic acid 7204298 7078550 6997270 7395893 8065964 8633902 7495809 8960336 8416981 11080785 10639244 11108758 12865946 12399849 14260376 14059548 14411361 16333719 15864429 17216284 15316132 15822855 16346097 17109724 16739805 17237519 19060326 18547872 23132412 21919901 22268581 24144721 S-Adenosylmethionine 34320915 41360858 36260206 48757069 35643511 36639826 41131379 43194899 40050620 30832134 45722810 44781213 39575985 42466823 47864992 50841219 52098915 57905801 40916935 42285433 55812716 45533348 52440991 70994011 60075938 43054390 52324378 49509294 71320481 42725972 37741136 72610624 S-Adenosylhomocysteine 6376234 8101551 7366569 8696813 6573144 6118972 6510784 7271512 7001803 6433538 7436635 8631482 6481998 7314183 7070335 6567194 7198567 8482743 6669764 6980578 9082722 7566722 7735260 9771196 12550657 12233003 11512955 10710711 12579677 12754874 10812315 16527129 gamma-Aminobutyric acid 75008060 74239343 71808320 73418372 62981506 65898154 77252008 73548233 50260543 48955742 58142097 58547146 85918842 89686219 97167998 89555267 117719635 134517833 119591857 110350526 69185678 71942167 80921614 88706258 82331979 63896201 63355039 59654379 78916930 81404535 67283084 90190250 N-Acetylarginine 606587 601454 707575 986213 632037 801744 671517 826585 801004 565621 766894 912948 747755 758901 750875 667468 629872 774748 589509 674337 815247 635411 908179 620323 1270625 902254 992462 1140364 1371190 1208458 1204501 1186725 N-Acetylaspartic acid 3972348 3795649 3894796 3834601 2842020 2790717 3295185 2528550 3867425 3458843 4465417 4466068 2940638 3157987 3185101 2917167 3163877 3365482 3282178 2608167 4054788 3951174 4538999 4468714 6393438 5383257 5218614 5071719 7405655 6905983 5712021 7508472 N-Acetylglutamine 836012 1215713 1181553 1125994 1216600 1062722 1224158 1070626 919935 939901 1062205 1158356 789199 853661 1042799 1013023 951167 1057103 877498 849318 907060 682531 994986 1066020 1370142 1157604 1079082 935241 2150072 2220264 1709449 1952356 N-Acetylglutamic acid 420545 432445 470000 451079 459044 696720 338594 285775 480968 447769 388626 371741 364650 435373 480171 369758 371707 395446 372659 355393 476672 446686 473823 408426 510727 620033 668806 571392 448357 476787 477943 561270 N-Acetylserine 933644564 954854874 992403556 986848158 889417490 836962142 1052033754 953104917 921489032 847484949 996431549 1064726355 805711014 836123481 839198846 781870375 939567872 1107831812 926549572 881069845 787587713 781719795 869743678 976192521 1265696486 1011948463 1007388153 977728887 1789149696 1752332410 1416607936 1946186725 N-Acetylleucine 3458956 3601911 4422462 4524471 4676121 3058034 4843351 4657909 4209973 5337967 3025165 5320581 5201462 4904710 4397500 6042523 4635054 6892014 5723336 4518171 6786296 6469004 5565764 6898999 496210 2902520 2337888 4816839 2783025 2761482 1018417 3321347 N-Acetylhistidine 15743682 16622954 16546734 19658969 13473132 13603453 16221178 16746846 15960238 14376674 19539577 19206588 12976165 13631655 12671955 13759939 11336600 13174013 9119176 11442114 13896650 12526660 16557017 17785822 29435610 26158408 27388652 25780536 37358603 35979586 30308274 41381152 N(6)-Methyllysine 12854285 14160602 13769880 14703636 12186947 13017532 12751881 11954996 12964495 11824130 13442481 14241542 11583940 12911903 11469860 10979503 13045193 13844581 11012231 12033649 15752743 13424880 15305648 16164765 20175712 19435501 17134151 16839374 19725353 16804964 14365866 20284472 N-Methyl-glutamic acid 3614567 3777958 5300474 6622191 3284879 4436081 3725959 6703358 4682868 4037233 4108703 4760622 3819265 5093362 6230261 4061418 4605020 5038859 5483594 6080361 4851676 4976043 7528396 8375277 5955899 6802270 6005228 6621110 5466691 4579604 3486782 5324577 N-Methylhistidine 38983560 45361794 41857727 52301377 34439918 44454779 46940699 50160310 45899969 33270569 44631098 49430592 32969365 36681255 37418135 40212723 31467940 34037206 27015421 28535743 41536112 33830016 42908776 51116878 86039555 68652922 69279113 74118163 118116754 94775253 81589668 109524417 N-Methylphenylalanine 7764301 9005816 9920740 8067017 9943075 12748868 8798458 8144018 7159693 8473316 6705310 8222232 6817924 5977566 8403049 7008386 9584097 10748224 9735927 10074272 7181840 7871315 9399692 9483343 5783547 6468443 5253188 5970402 6636978 7750123 6363695 6565615 Dimethylarginine 60454583 70994391 56628186 71014831 50382767 59218348 57825895 61476159 73209245 58592310 65685766 75412671 51250469 55072293 52978636 60157700 47543800 46200601 38380923 49675338 61154702 48038384 58816960 66434067 91467987 90444723 79857478 84313344 82243614 76412103 76970542 85478460 N(6),N(6)-Dimethyl-lysine 7673886 9459514 8556532 10055429 7152831 8065956 8263312 8969033 9005167 8013925 9133906 9002209 5897159 7508344 7403429 6940570 7850769 8702425 6319547 7560738 7930196 7719969 9400565 9898543 13299400 12771732 11439409 12353086 13137050 11896691 11886696 12515647 N-6-Trimethyllysine 38525122 41427622 35924220 43149817 32583923 41861224 37107953 42272886 40843890 35809110 37667738 38978237 31967561 38778707 41570245 38574700 36405635 36709877 31155596 38552142 41543750 36063575 44249926 47427768 55288965 52609510 49651601 52212363 60257921 57723156 54280562 64021707 N8-Acetylspermidine 169821748 174164660 161499702 180194526 128438326 155806278 164837618 195547292 201965177 171366884 211420486 229037360 109691610 110968690 120731619 126462298 143442185 145547337 113617494 147752150 166975725 140517903 187776279 216607888 306898946 222474704 262417196 242145375 194399819 169817173 127794073 207033032 Acrylic acid/Pyruvaldehyde 5212440 6387859 5572247 6070942 5745119 6414643 3255801 3494499 3823866 3404610 4704356 3252180 2191370 2937866 3618869 3500148 1936941 1620375 2204786 1608864 2566563 2539434 3816563 2654488 4126215 4216988 4598104 4524270 4196265 3382604 2603753 3086975 Adipic acid/2-Methylglutaric acid 2884340 3375103 5315832 3346153 2303268 3232393 381791 2005936 3985001 3376528 2592414 2537199 3167867 2129705 2291513 3000829 2600520 2891310 2427639 2907466 2641754 1576619 2442797 2540921 2399922 3906699 3081610 3205306 2111131 3391514 2424540 3283712 Cysteic acid 147437 218262 189730 184341 137158 254316 177724 169635 236610 192247 101870 235515 244919 272699 235300 282993 162399 197194 147876 154046 180371 177795 229674 227988 346155 212234 207787 363916 345157 329569 292004 354648 Crotonic acid 11261657 12404740 12089198 11476403 9376308 9477796 13193281 11480377 7138758 7332003 10298018 8939155 13411274 15033445 15411060 14043258 19544184 23870497 18634755 17268330 11331348 11529726 13493364 13428179 13091792 9354523 10347237 10940911 11234849 13655696 11456139 15132079 Citraconic acid 2327133 2783644 2848593 2934641 1888307 2277266 2308661 2783271 2657439 1987799 2965923 2618566 1889356 2245466 2480253 2064789 2410815 2053684 2105220 1882786 2419912 1990352 2686398 2642257 3133154 3054351 3030390 3016185 4564657 4211422 3593158 5245313 Citramalic acid 8623617 9223890 8595728 8890802 6915521 7718441 7664458 7825612 6916584 6186248 7315788 7262997 5773493 6130255 6158772 5800108 7706744 8523800 7389408 6851322 6706368 6605401 6972728 8279922 10010021 8370976 8441464 8134782 15062335 13814358 11483429 16150668 Glycolic acid 10703369 10709426 11512869 13633155 14314973 12176748 11407476 12336822 12380187 12335692 11473551 14781995 11757030 12513323 12903440 12859054 13007362 13031735 12797525 11415443 13123322 14059610 12711703 12667542 13192934 13344157 13241052 13178114 15077330 14002075 12868187 12826966 Glyceric acid 131180 110026 301051 172631 n.a. 170681 n.a. 135865 205059 141860 166588 122299 103621 162094 177743 253163 225522 188015 105289 194177 189561 174616 140555 195776 170459 228698 169979 186686 170049 171932 95161 144780 Galactaric acid 129540 138476 199249 153177 133976 109329 124509 137781 146470 122456 108603 116334 118803 174281 135090 139415 154000 145801 130969 157379 125932 121351 78519 118748 51335 25463 126235 54144 62332 70123 92294 87730 Pipecolic acid 4360442 4754774 4874870 4830015 4258040 3749400 4813985 4043544 4243422 4112557 4428979 3665215 3743658 3845886 4223411 4696827 5052920 5103686 3991536 4424642 4401745 4718731 4723237 4946929 6081111 5632934 5409265 5392668 6461215 5908845 5638421 7179463 Quinic acid 632917 601395 689836 634138 560440 816717 567167 504114 583204 637504 544238 525223 509185 681529 679639 593776 879593 1000380 942859 1002327 677209 643414 927783 799088 686473 798315 700549 656252 710388 605371 640784 785114 Salicylic acid 12758031 14075810 12774515 13123925 13091132 13130294 13244325 13465968 13765477 13750632 12708273 13959849 15128969 14551571 13333357 13755898 15786313 14685515 15286359 14677310 13466576 13553779 14248170 13963509 14011570 13130430 14129621 12854351 18734827 17100309 15447759 19310289 Pyroglutamic acid 520124333 583578599 528597026 614915824 484756661 527048179 620280702 618566577 535876693 478044775 557376645 588886458 500301459 505545366 529499318 566180405 413222905 414479969 336018074 375461918 520350182 451172140 547386359 617700023 804110746 664223486 665209424 704945661 930946225 859747685 722431996 986691582 3-Methyl pyruvic acid 3440798 5326930 4431045 4746978 5149784 4134875 2844941 2463173 3188911 3537788 3334376 2315265 1904908 3183570 2840968 2401715 1357767 1912158 2435744 1541020 3413308 2296684 3387037 2849212 4181942 2720599 3227125 4153250 2883307 2616196 1954546 2535814 3-Hydroxyanthranilic acid 1182462 1343623 1078533 599253 690707 954219 860562 883127 1057792 307882 717223 692032 573533 1227625 1013627 1559781 413573 820406 546221 518088 550040 439583 481275 1006448 704511 855019 1235631 781872 1113057 884974 868510 1234323 cis-4-Hydroxyproline 1282132532 1365514248 1307101547 1443847104 1089387887 1232753047 1321368822 1367423937 1294876314 1154836247 1297781708 1457638774 1125399580 1240783894 1314653638 1278771427 1219320589 1303258642 1160941319 1266362034 1304004486 1191189518 1478343651 1517306140 1527561623 1349076834 1389483507 1469684006 1953854411 1737219805 1580983380 2004464204 2-Hydroxy-3-methylbutyric acid 20051297 21952358 19126328 21869325 16806361 17843516 19241191 18541468 23788407 20243274 23097447 24402470 22249714 24597470 25076290 24542096 23012550 22978828 21267639 21755705 25237975 23396107 27622495 30472727 31425453 28070757 28125024 27791135 43365793 40386589 37090144 45676746 4-Imidazolone-5-propionic acid/Imidazolelactic acid 599326 872804 459215 430402 115146 420020 590351 903876 405460 587178 757663 915724 606536 382804 605449 710919 165511 308923 98875 288484 509387 556964 630255 750580 823052 836750 883248 866894 1250468 1475534 500640 1472644 4-Acetamidobutanoic acid 6550453 7833302 7622762 8535728 7956717 8640860 7242924 6584634 8064907 6464651 7351894 7406294 5807876 5757195 6230789 6318321 7003295 6376928 6433108 5812985 7683447 6038201 7301631 7621749 9415334 9188119 8987802 8891943 10152054 8714400 7310838 10102410 5-Aminopentanoic acid 819964360 778833177 880590648 890239284 664710519 711432446 794198777 741371472 680564406 657095220 720473553 692464659 678058090 669311293 706816508 725017795 834459545 908538186 695459293 775840780 694385121 641326603 783312533 839058024 930674135 786859151 863016414 886726064 1091190615 961270578 824886136 1139369751 4-Hydroxybenzoic acid/Dihydroxybenzaldehyde 12758031 14075810 12774515 13123925 13091132 13130294 13244325 13465968 13765477 13750632 12708273 13959849 15128969 14551571 13333357 13755898 15786313 14685515 15286359 14677310 13466576 13553779 14317793 13963509 14011570 13141646 14129621 12854351 18734827 17100309 15447759 19310289 4-Hydroxybenzaldehyde 1107361453 947250245 1015954324 881039893 1094145584 1061207738 1427665097 905005592 1234991246 1483911063 1129192958 1435562977 1253589169 1411969695 1552134392 1363584889 1363179077 1406893726 1518906440 1521865275 1337779573 1154629500 1664575465 1476696067 1909054108 1985489526 1628478299 1774128873 1695221001 1675393222 1503769926 1722832999 DOPA 15624937 16531600 16440725 19577899 13473132 13544453 16081606 16701598 15960238 14376674 19470743 18912313 12917321 13577474 12597735 13759939 11219577 13067084 9011740 11342402 13896650 12526660 16557017 17785822 29310567 26064448 27307956 25662610 37227044 35841503 30192669 41186530 Methylimidazoleacetic acid 3858872 3468665 3892682 3865356 3142463 2856907 3425085 3350779 3009853 3369666 3649338 3541442 2488996 2828579 2685243 2742498 6118154 6773020 6662156 6093850 3883573 3877566 4115140 4622188 5116061 4113971 4683463 4398784 4958859 4883666 4136853 5394332 Benzoic acid 1107361453 947250245 1015954324 881039893 1094145584 1061207738 1427665097 905005592 1234991246 1483911063 1129192958 1435562977 1253589169 1411969695 1552134392 1363584889 1363179077 1406893726 1518906440 1521865275 1337779573 1154629500 1664575465 1476696067 1909054108 1985489526 1628478299 1774128873 1695221001 1675393222 1503769926 1722832999 Imidazole 4540464 4766878 3824982 6260834 5591224 4826082 4052272 3792159 4379819 4987857 4826643 4790437 4700026 7176869 5494248 5553889 4374548 3973427 4837003 3543291 4988135 6895636 5037557 5528636 5977329 4952199 4523584 4911285 5001465 4990373 3328871 4681172 Phenol 1742811 1914272 1879603 1553380 1547945 1862899 1366683 1494469 1170752 2037324 1632079 1978707 1865453 1557155 1755061 1833899 1845941 1890702 2057459 1966902 1659424 1927509 2162369 1605877 2024593 1953694 1645845 1555849 2420491 2314164 2013980 2583597 Toluene 212476 507604 313850 302647 439224 324691 343074 300789 254943 305659 195428 118775 243588 259119 247216 394668 491577 457221 375330 419757 282964 184186 500065 456195 384804 362885 254217 414074 358824 412354 249028 336109 Xylene 8137675 7358443 6747042 10843660 13259885 6112345 13888816 11503467 8723977 14060954 7685788 11954230 13025789 15229237 10517275 15327551 13166676 19723058 14278663 12546818 16831101 18332649 14736649 16990402 508366 11530066 10893408 17468840 8882261 9152797 3218452 12908040 Asp-Glu 211645 170091 318687 313656 223558 264212 373371 345746 372459 223213 403083 321278 266275 300250 310402 256778 395068 457534 352230 297900 421235 374399 458138 462939 674497 412622 470178 411664 628339 462056 445097 670872 Argininosuccinic acid 5053088 6760176 6130369 8576467 5274839 6983971 7662471 7956779 6452093 5916111 6756969 7180165 3720791 4429346 4126520 4152543 6582925 6192908 4605000 6316505 6683163 5221497 7253134 8723859 15903088 14631329 14044439 13303165 12678629 11491832 10925861 12978090 Isovalerylglycine 1222908 1167705 1178679 1205646 1089608 940869 1083390 847953 1277284 1212237 1400901 1410294 1170351 1252259 1209844 1212435 1263693 1494100 1203421 1160958 1311999 1302439 1382074 1561143 1753625 1626761 1622532 1371625 2208340 2385420 1883810 2652268 O-Acetyl-homoserine 689163 668508 834499 956049 815352 939874 994564 771945 647644 753496 753367 1015635 556322 574757 671757 596187 628953 702995 667789 670753 707201 718580 629802 724831 1063820 1073009 943033 943940 1529020 1179677 1229692 1499526 N-Acetylmethionine 9334140 8077290 8988622 8425942 7679388 7499307 8674765 6835576 8769493 8796629 9106842 10393594 6925582 7899432 7980841 6727881 10099010 12451221 11178706 9216613 9902599 10359840 11611659 11449342 14051029 11582394 11306402 11160355 20333882 20376711 17041105 23397608 N-Acetylputrescine 9441589 13360337 9768888 12106036 10735219 9461180 11226737 15834015 15443415 10814472 13161980 12326493 10007713 10015989 11047596 12802564 4435511 4453995 5431853 4818021 10406187 9715672 10982490 12788908 12507339 13430178 10709724 10010583 2750504 1894907 2144073 2399367 Sphingosine 1956329 2564075 2069104 3853433 3522248 2777103 2105015 2795941 2628702 3039816 2310136 2456845 3122165 4189156 3121126 1882351 3134016 2616561 2317522 2175973 2491890 1704158 3460217 1550491 1828805 3194865 2775722 2929812 3694410 3666649 2663294 3027909 Pseudouridine 732724 796957 668545 706895 816873 819477 622596 594573 712330 646937 714963 631951 587960 581256 634433 640209 467102 438596 432432 498766 633096 574918 756329 720184 832942 841738 787016 827212 918144 758658 657981 832611 4-Guanidinobutanoic acid 19026524 19170786 21162829 21571705 18720221 18028163 22428329 20731686 16845357 14253602 18272445 18343533 14806432 16382355 16704031 15257308 35722490 40998632 38512101 35599952 21027610 20168186 23547955 24951473 26789327 21634823 24863964 21939647 25552580 23437674 21214751 27333986 3-Methyl-2-oxovaleric acid 29082614 27594135 27512768 31198065 22658524 21958674 30379625 27930619 21941424 20825586 26521459 27846897 17369333 18525652 21366106 18343553 24435855 30260656 27580838 25519916 17734127 17724985 19915633 21157885 33695829 26033159 28162860 27203975 44256935 44102060 37886361 47991888 Dihydrouracil 6903775 7381796 6961235 8452501 6496742 6850664 7527498 8501488 8393160 7209413 8153942 7824154 6229586 6381974 7105931 7151201 5293116 4647471 4596014 4636925 7266269 6521518 7382466 8102742 9972541 8859014 9134690 9352832 12019710 11495992 10066486 11102947 5'-Methylthioadenosine 191145531 208630515 191803336 216434575 196415408 182052808 192387622 182703664 187913773 163925571 179385931 195538821 185405507 187623599 201359983 208685659 169842451 186081728 160913323 178134206 223497716 196590831 221148388 247698153 222032749 210790525 208603703 206112281 224574690 187879509 172373235 232619348 Acisoga 217327084 208012973 228365296 202619421 233291326 226973338 233916662 217278605 214453610 234135257 241745316 236652218 296309460 291991659 284868043 281537675 295054408 309635564 334303274 310874573 269362149 290741283 266540497 258933421 208508599 218045454 222964373 219148606 224679597 245131171 257103721 219410597 Allantoin 15856836 16959893 17102611 18564294 15525426 17446311 17223828 15772329 16813846 14390370 15255559 16617038 13065673 14050160 15051205 15117114 16800997 17012162 15411555 15397805 15589761 13673284 17326206 18405386 21807552 19351800 19873622 19754704 23686551 21019009 17774023 24191156 Dimethylglycine 75008060 74239343 71808320 73418372 62981506 65898154 77252008 73548233 50260543 48955742 58142097 58547146 85918842 89686219 97167998 89555267 117719635 134517833 119591857 110350526 69185678 71942167 80921614 88706258 82331979 63896201 63355039 59654379 78916930 81404535 67283084 90190250 gamma-Glutamylalanine 150217 198217 159916 539298 118802 388786 65097 101400 310477 149607 76672 162778 101876 233833 113710 91550 2184604 2508469 2078813 2349894 863170 546307 196030 823958 277380 534843 720923 167289 395825 345351 348848 587349 gamma-Glutamylhistidine 399384 406390 445864 481620 327309 298251 391939 521427 358700 373540 347044 508830 313869 403469 369761 490794 247053 216933 256600 241380 446329 384483 505202 504064 548372 496990 579283 534284 1015797 1073255 833280 1126803 gamma-Glutamylmethionine 86775 67438 138474 143572 62432 90311 112864 163962 99439 121569 107388 106207 107270 112112 130727 165683 164774 289608 169924 202305 148803 121038 168909 221375 232274 199235 190845 189790 168664 142851 149342 304855 Guanidoacetic acid 4694692 5505696 5013726 6654357 3307687 4138947 4680067 5820175 5384565 4283443 5079772 6639567 14678689 15014638 14115313 16797152 1496931 1475449 973031 1517888 3926410 3125575 4692692 5325989 6816950 6074710 5505341 6340637 8345027 4871428 6606914 6192128 Guanidinosuccinic acid 6239254 7266796 7524431 7948414 7354552 7546931 8389156 8489573 7026497 6567737 10880084 7536360 6431220 7518562 7371612 8335150 5685988 6511659 5661404 5663956 5825567 6006305 6607471 7079182 10169905 8614222 8937809 8947114 10269538 9329519 9840876 10081682 Homocitrulline 169930 515153 522801 588489 312632 268722 379929 357158 459662 380122 299723 403755 243882 211482 328657 227587 85820 43082 27006 71975 220146 90161 336630 500665 972176 601336 629730 794474 848496 1006527 612034 928237 CAR 4:0;3Me 167510846 177300023 175691880 189803254 175468473 196285367 225075196 208364061 146908086 144937285 174775709 164165542 246773621 265814853 258589534 255550795 182495475 201028532 177399903 179046151 159780557 159934060 186305295 197867319 245939588 178521903 186111076 190237007 276162382 274032754 218530141 288535917 Leucylglycine/N(2)-Acetyllysine 39112813 41892195 41042370 38165797 37921726 40212660 38837468 36015234 42140308 36766807 39841354 43019000 35002544 39870503 36394940 34236168 42363848 43309542 36314544 38250504 40902562 37752250 41209273 45140270 50622356 50418931 49567585 53759477 58828054 55663679 45606888 61220600 Methylsuccinic acid 15949604 13290469 9999535 9986643 8581054 10770075 9969701 9103043 11661781 7096474 15095397 9725229 8289944 8747777 12951527 7580184 10196103 11360554 8965112 7635678 13495471 15561457 9654897 14382100 17355045 12654742 14364867 11315571 17285867 18229080 14843832 14636600 N-Acetylneuraminic acid 4060148 4319770 3413090 4680586 3483139 3748550 2342239 2658925 2959706 2708864 3889098 2796986 1570892 2046655 2690976 2884723 2321282 1460247 2129829 1870993 2939940 2346944 4315348 3349659 6117978 4836716 5293993 5589752 7334422 4304423 3480500 4857833 N-Acetyltaurine 958921 962359 1238332 1273426 837486 1773434 1137569 1048273 776766 1187831 1864723 1320229 1282713 1540585 1494387 895814 2085601 2298029 1971745 1719940 1681992 1498833 1788117 1602751 1552439 1245559 1332914 1296944 2191356 2177856 1846172 3033539 N-Acetyl-tryptophan 715592 628544 651651 767379 889886 811904 1000741 934063 609601 529796 560150 548120 400835 379353 466510 437572 869317 859196 755193 786969 782690 594505 645026 915681 892090 712680 690936 704149 1218901 1166640 898376 1310670 N(5)-Acetyl-ornithine 1806690 2283914 1896682 2644433 2035345 2077308 1993692 2549245 2439346 2113550 2358090 2623062 1735409 1811438 1862598 2036485 1403624 1403338 1020913 1320766 1846626 1787155 2066317 2613438 3976371 3552187 3358802 3955438 5365593 4877210 4327692 5335962 O-cresol sulfate/P-cresol sulfate 925885 748215 930858 786488 663902 875181 540256 559561 912391 881367 730481 961947 682535 828027 895874 757738 851282 868701 716288 731750 1128401 1040760 1086162 1149817 1318729 1220399 1277510 1292116 1314550 1307585 1138630 1461171 Orotidine 3121990 2353561 3128780 2406890 2085378 2091291 2249020 1770951 1987029 1990553 2275742 2038953 2194440 2157899 1989653 2136846 2309595 2549064 2526767 1919860 1733723 1940506 2023313 1867002 2724342 2215045 2569953 2421945 5734908 5583877 4989105 6591896 Phenyl hydrogen sulfate 3031722 2960694 2708014 2938603 2155194 2481951 2021467 1962229 3747784 3008049 2493929 2861360 2023517 2265437 2322424 2269053 4674455 4090835 3328766 2902398 4480070 4752852 4314826 4569134 6332404 5182065 5149845 5638019 4611558 3815834 3279615 3675159 Phenylacetylglycine 40005768 29520836 30756131 32805857 25580681 24398535 20207663 18326990 36917494 29530243 28686960 27024237 24831026 27290036 25753354 25831994 16938242 15141596 11777685 11245779 34400566 25136814 26394052 28772015 31697472 25448669 24062512 25881471 25092828 19880294 15060574 18551113 Pro-hydroxyPro 2749520 3741024 3078732 3554166 3125259 3506106 3047963 2528851 2670536 2536261 3029738 3123358 2159717 2207403 2850033 2680933 1490030 1138853 1055116 1667145 2299212 2080875 3004491 3398161 5402863 4854170 4365791 4856113 4207681 3154457 2351441 4227714 1-Pyrroline-5-carboxylic acid 755912 552671 384335 367976 394046 495879 568659 596027 265170 389548 480699 396300 442981 595722 464021 507716 277712 299825 328084 369873 417254 436239 373529 352006 346510 439696 649802 369359 800814 798656 550138 521491 Sucrose/Maltose 4915027 5926460 6745650 6122269 6888818 9086103 5164807 4532269 6652623 6310676 6281493 5788042 5173695 6152768 6080876 5977521 4897945 4390023 3878473 4257822 6242626 5479702 6761661 5863268 6274511 8060980 7023519 6759041 4010215 4025940 4550134 4373090 Urocanic acid 3833694 4692451 3407483 3674291 2935797 3604176 3692332 3154442 3638417 2578804 3414109 3395362 2801471 2425418 2882691 2676261 3246899 3364145 1789014 3338230 4141550 3580315 3512761 3159232 4696544 5515634 3450885 4435185 4671267 6606890 3360993 4274290 Ala-Leu 5384496 6267446 5051043 6033675 4193579 4828858 5246069 5411821 5177666 4344077 5218142 5546099 5106855 5587311 6290558 5666749 6044052 6142193 5673110 5810340 5312690 4635542 5486909 6415406 4990591 4647452 5040350 4590819 5404539 4525994 4128739 5147024 N-Carboxyethyl-gamma-aminobutyric acid 9972331 10448260 10120636 12961715 7810370 8552141 10263807 10621432 9841569 8351564 11703832 13020783 7093731 7206057 7262173 7373324 6631609 7764090 5678846 6692660 7483838 6931520 9338109 10851949 21316162 16944882 16676765 16322998 36400514 31915149 23390398 39099726 FMN 15769652 18265006 20314683 19054508 21845474 26496970 16383157 15546260 21312859 20163218 19516659 18663194 17640455 19457856 19810462 19210386 15503897 13144876 13368115 14878749 18708711 17311493 19573002 17965539 18930501 22098137 19991531 19897374 11617231 11777348 13317153 12075069 Leu-Gln 1380233 1302632 1563306 1472683 1195331 2130988 881557 1051963 1162402 967496 1469498 1122963 712564 1005527 1064863 733666 621209 639436 621217 714873 977990 847832 1561837 1036047 1678399 1273768 1206562 1293626 1329151 750314 704301 1094371 Methanolphosphate 2683616 2588376 3116293 2958400 2651287 2875830 3162655 3297068 3031749 3455805 2763888 3747469 2926762 3481595 4161143 3359748 3327912 3321681 4120539 4213972 3303471 3103713 4340641 4933366 3837258 4358392 4012581 4174087 3755124 3693604 3435326 4413840 6-Dimethylaminopurine 195362688 205421963 219685182 217258700 241286648 229886957 255333944 244601312 188612998 174706712 177507818 197955499 195399580 194868234 191883920 191661149 320130780 346898564 335811144 350123455 222566562 199924676 232996969 260930034 324467635 270094648 257838234 289783011 472388375 368566747 301808244 407382486 Aspartylglycosamine 898707 1055078 860087 1145964 768835 917647 1027850 1131694 1087836 806227 1007994 1075943 840019 903193 1056401 917922 944969 1099140 966973 937436 833347 838878 919611 1097402 1093169 923195 992828 1065478 1169522 1051439 974957 1123236 Pro-Gly 3661477 4481855 4360097 2988431 4834467 3713241 3399560 4788012 4004690 4206862 3446247 4539802 3904569 3904654 3960898 4136372 4008927 6614121 2755293 3711187 5653919 4391418 5963910 3911060 8197684 5792779 5343523 5900675 7244639 6010902 5126191 6692452 Pyridoxal 12601560 13395643 12069216 12852088 12384047 12918384 12357192 13294620 12284376 10452284 11301749 11461926 12682262 12833856 12201002 12639737 13213279 12589615 11722298 14457617 11264217 10869802 12711435 13539890 15156311 13182464 13322830 12159744 18188024 17922708 14814942 18597906 Cinnamoylglycine 427418 504433 655684 478372 513551 496193 449514 450076 344662 401106 304350 480542 630243 617950 548276 545927 296718 144583 264111 184570 424034 409001 541248 624118 692154 803923 539460 676072 941056 860546 759080 853627 Hippuric acid 2688637 3384520 2966962 3560667 3201735 3514777 2291598 2442842 3316096 2665102 2616792 2909126 2171817 2474863 2460233 2712849 2242915 1818288 1973222 1953488 2488506 2479809 2636831 3115385 3938282 3645604 3361308 3437719 3465074 2526075 2387756 3323655 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Internal name Ion Adduct Formula Exact mass Super class Main class Sub class Glucose/Fructose/Mannose GNE001_Glucose_Fructose_Mannose_Neg [M-H]- C6H12O6 180.0634 Carbohydrates Monosaccharides Hexoses Glucose 6-phosphate GNE002_Glucose-6-phosphate_Neg [M-H]- C6H13O9P 260.0297 Carbohydrates Monosaccharides Hexose phosphates Fructose 6-phosphate GNE003_Fructose-6-phosphate_Neg [M-H]- C6H13O9P 260.0297 Carbohydrates Monosaccharides Hexose phosphates Fructose 1,6-bisphosphate GNE004_Fructose-1,6-bisphosphate_Neg [M-H]- C6H14O12P2 339.9961 Carbohydrates Monosaccharides Hexose phosphates Glyceraldehyde 3-phosphate/Dihydroxyacetone phosphate GNE005_Glyceraldehyde-3-phosphate_DHAP_Neg [M-H]- C3H7O6P 169.998 Organic acids Short-chain acids Short-chain acids 2-Phosphoglyceric acid/3-Phosphoglyceric acid GNE007_2-Phosphoglycerate_3PGA_Neg [M-H]- C3H7O7P 185.9929 Organic acids Short-chain acids Short-chain acids Phosphoenolpyruvic acid GNE009_Phosphoenolpyruvate_Neg [M-H]- C3H5O6P 167.9824 Organic acids Short-chain acids Short-chain acids Pyruvic acid GNE015_Pyruvate_Neg [M-H]- C3H4O3 88.016 Organic acids Short-chain acids Short-chain acids Succinic acid/4-Hydroxy-2-Oxobutanoate GNE016_Succinate_4-Hydroxy-2-Oxobutanoate_Neg [M-H]- C4H6O4 118.0266 Organic acids TCA acids TCA acids Alanine/Sarcosine GNE021_Alanine_N-Methylglycine_Pos [M+H]+ C3H7NO2 89.0477 Organic acids Amino acids and peptides Amino acids Arginine GNE022_Arginine_Pos [M+H]+ C6H14N4O2 174.1117 Organic acids Amino acids and peptides Amino acids Asparagine/Ureidopropionic acid GNE023_Asparagine_3-Ureidopropionate_Pos [M+H]+ C4H8N2O3 132.0535 Organic acids Amino acids and peptides Amino acids/Ureas Aspartic acid GNE024_Aspartate_Pos [M+H]+ C4H7NO4 133.0375 Organic acids Amino acids and peptides Amino acids Glutamine GNE026_Glutamine_Pos [M+H]+ C5H10N2O3 146.0691 Organic acids Amino acids and peptides Amino acids Glutamic acid GNE027_Glutamate_Pos [M+H]+ C5H9NO4 147.0532 Organic acids Amino acids and peptides Amino acids Glycine GNE028_Glycine_Pos [M+H]+ C2H5NO2 75.032 Organic acids Amino acids and peptides Amino acids Histidine GNE029_Histidine_Pos [M+H]+ C6H9N3O2 155.0695 Organic acids Amino acids and peptides Amino acids Isoleucine GNE030_Isoleucine_Pos [M+H]+ C6H13NO2 131.0946 Organic acids Amino acids and peptides Amino acids Leucine GNE031_Leucine_Pos [M+H]+ C6H13NO2 131.0946 Organic acids Amino acids and peptides Amino acids Lysine GNE032_Lysine_Pos [M+H]+ C6H14N2O2 146.1055 Organic acids Amino acids and peptides Amino acids Methionine GNE033_Methionine_Pos [M+H]+ C5H11NO2S 149.0511 Organic acids Amino acids and peptides Amino acids Proline GNE034_Proline_Pos [M+H]+ C5H9NO2 115.0633 Organic acids Amino acids and peptides Amino acids Serine GNE035_Serine_Pos [M+H]+ C3H7NO3 105.0426 Organic acids Amino acids and peptides Amino acids Valine GNE036_Valine_Pos [M+H]+ C5H11NO2 117.079 Organic acids Amino acids and peptides Amino acids Threonine/Homoserine GNE037_Threonine_Homoserine_Pos [M+H]+ C4H9NO3 119.0582 Organic acids Amino acids and peptides Amino acids Tyrosine GNE038_Tyrosine_Pos [M+H]+ C9H11NO3 181.0739 Organic acids Amino acids and peptides Amino acids Tryptophan GNE040_Tryptophan_Pos [M+H]+ C11H12N2O2 204.0899 Organic acids Amino acids and peptides Amino acids Kynurenine GNE043_Kynurenine_Pos [M+H]+ C10H12N2O3 208.0848 Organic acids Amino acids and peptides Amino acids Kynurenic acid GNE044_Kynurenic acid_Pos [M+H]+ C10H7NO3 189.0426 Alkaloids Anthranilic acid alkaloids Acridone alkaloids Niacinamide GNE045_Nicotinamide_Pos [M+H]+ C6H6N2O 122.048 Alkaloids Pyridine alkaloids Nicotinic acid alkaloids Phenylalanine GNE047_Phenylalanine_Pos [M+H]+ C9H11NO2 165.079 Organic acids Amino acids and peptides Amino acids Indole GNE055_Indole_Pos [M+H]+ C8H7N 117.0578 Alkaloids Tryptophan alkaloids Simple indole alkaloids Indole-3-lactic acid GNE058_Indole-3-lactic acid_Neg [M-H]- C11H11NO3 205.0739 Alkaloids Tryptophan alkaloids Indolecarboxylic acids Propionic acid/Lactaldehyde GNE067_Propionate_Lactaldehyde_Neg [M-H]- C3H6O2 74.0368 Fatty Acyls Fatty acids Saturated FA 2-Hydroxybutyric acid/3-Hydroxyisobutyric acid GNE071_2-Hydroxybutyrate_3-Hydroxyisobutyrate_Neg [M-H]- C4H8O3 104.0473 Fatty Acyls Fatty acids Hydroxy FA Caproic acid/Isocaproic acid GNE076_Caproate_Iso-caproate_Neg [M-H]- C6H12O2 116.0837 Fatty Acyls Fatty acids Saturated FA/Branched FA Glycocholic acid GNE080_Glycocholic Acid_Neg [M-H]- C26H43NO6 465.309 Sterol Lipids Bile acids C24 Bile acids Taurodeoxycholic acid/Taurochenodeoxycholic acid GNE086_Taurodeoxycholic Acid_Taurochenodeoxycholic Acid_Neg [M-H]- C26H45NO6S 499.2968 Sterol Lipids Bile acids C24 Bile acids Cortol GNE094_Cortol_Pos [M+H]+ C21H36O5 368.2563 Sterol Lipids Steroids C21 Steroids Cortisol GNE095_Cortisol_Pos [M+H]+ C21H30O5 362.2093 Sterol Lipids Steroids C21 Steroids Adenine GNE100_Adenine_Pos [M+H]+ C5H5N5 135.0545 Nucleic acids Purines Aminopurines Uracil GNE103_Uracil_Neg [M-H]- C4H4N2O2 112.0273 Nucleic acids Pyrimidines Other pyrimidines Xanthine GNE105_Xanthine_Neg [M-H]- C5H4N4O2 152.0334 Nucleic acids Purines Xanthines Adenosine/Deoxyguanosine GNE107_Adenosine_Deoxyguanosine_Pos [M+H]+ C10H13N5O4 267.0968 Nucleic acids Purines Purine ribonucleosides Uridine GNE110_Uridine_Neg [M-H]- C9H12N2O6 244.0695 Nucleic acids Pyrimidines Pyrimidine ribonucleosides Cytidine GNE112_Cytidine_Pos [M+H]+ C9H13N3O5 243.0855 Nucleic acids Pyrimidines Pyrimidine ribonucleosides Thymidine GNE113_Thymidine_Pos [M+H]+ C10H14N2O5 242.0903 Nucleic acids Pyrimidines Pyrimidine deoxyribonucleosides Inosine GNE114_Inosine_Neg [M-H]- C10H12N4O5 268.0808 Nucleic acids Purines Purine ribonucleosides Xanthosine GNE115_Xanthosine_Neg [M-H]- C10H12N4O6 284.0757 Nucleic acids Purines Purine ribonucleosides Deoxyuridine GNE119_Deoxyuridine_Neg [M-H]- C9H12N2O5 228.0746 Nucleic acids Pyrimidines Pyrimidine deoxyribonucleosides AMP GNE121_AMP_Pos [M+H]+ C10H14N5O7P 347.0631 Nucleic acids Purines Purine rNMP GMP GNE122_GMP_Neg [M-H]- C10H14N5O8P 363.058 Nucleic acids Purines Purine rNMP UMP GNE123_UMP_Neg [M-H]- C9H13N2O9P 324.0359 Nucleic acids Pyrimidines Pyrimidine rNMP CMP GNE124_CMP_Neg [M-H]- C9H14N3O8P 323.0519 Nucleic acids Pyrimidines Pyrimidine rNMP IMP GNE125_IMP_Neg [M-H]- C10H13N4O8P 348.0471 Nucleic acids Purines Purine rNMP Arabitol GNE132_Arabitol_Neg [M-H]- C5H12O5 152.0685 Carbohydrates Monosaccharides Sugar alcohols Sorbitol/Mannitol GNE133_Glucitol_Mannitol_Neg [M-H]- C6H14O6 182.079 Carbohydrates Monosaccharides Sugar alcohols Erythrose GNE134_Erythrose_Neg [M-H]- C4H8O4 120.0423 Carbohydrates Monosaccharides Tetroses Ribose GNE137_Ribose_Arabinose_Neg [M-H]- C5H10O5 150.0528 Carbohydrates Monosaccharides Pentoses Sedoheptulose GNE138_Sedoheptulose_Neg [M-H]- C7H14O7 210.074 Carbohydrates Monosaccharides Monosaccharides Fucose 1-phosphate GNE141_Fucose-1-phosphate_Neg [M-H]- C6H13O8P 244.0348 Carbohydrates Monosaccharides Hexose phosphates Ribose 5-phosphate GNE142_Ribose-5-phosphate_Neg [M-H]- C5H11O8P 230.0192 Carbohydrates Monosaccharides Pentose phosphates Sedoheptulose 7-phosphate GNE143_Sedoheptulose-7-phosphate_Neg [M-H]- C7H15O10P 290.0403 Carbohydrates Monosaccharides Monosaccharide phosphates Glucosamine 6-phosphate GNE146_Glucoasmine-6-phosphate_Neg [M-H]- C6H14NO8P 259.0457 Carbohydrates Monosaccharides Amino sugars N-Acetyl-glucosamine 6-phosphate GNE147_N-Acetylglucosamine-6-phosphate_Neg [M-H]- C8H16NO9P 301.0563 Carbohydrates Monosaccharides Amino sugars GDP-L-fucose GNE148_GDP-fucose_Pos [M+H]+ C16H25N5O15P2 589.0822 Nucleic acids Purines Purine rNDP GDP-mannose GNE149_GDP-mannose_Pos [M+H]+ C16H25N5O16P2 605.0772 Nucleic acids Purines Purine rNDP UDP-glucose/UDP-galactose GNE150_UDP-glucose_UDP-galactose_Pos [M+H]+ C15H24N2O17P2 566.055 Nucleic acids Pyrimidines Pyrimidine rNDP UDP-N-acetylglucosamine GNE152_UDP-GlcNAc_Neg [M-H]- C17H27N3O17P2 607.0816 Nucleic acids Pyrimidines Pyrimidine rNDP Urea GNE153_Urea_Pos [M+H]+ CH4N2O 60.0324 Organic acids Organic carbonic acids Ureas Orotic acid GNE155_Orotate_Neg [M-H]- C5H4N2O4 156.0171 Nucleic acids Pyrimidines Pyrimidine carboxylic acids Ornithine GNE156_Ornithine_Neg [M-H]- C5H12N2O2 132.0899 Organic acids Amino acids and peptides Amino acids Creatine GNE157_Creatine_Pos [M+H]+ C4H9N3O2 131.0695 Organic acids Amino acids and peptides Amino acids Creatinine GNE158_Creatinine_Neg [M-H]- C4H7N3O 113.0589 Organoheterocyclic compounds Azoles Imidazolines Citrulline GNE159_Citrulline_Pos [M+H]+ C6H13N3O3 175.0957 Organic acids Amino acids and peptides Amino acids Putrescine GNE160_Putrescine_Pos [M+H]+ C4H12N2 88.1 Fatty Acyls Fatty amines Monoalkylamines Butylamine GNE167_Butylamine_Pos [M+H]+ C4H11N 73.0891 Fatty Acyls Fatty amines Monoalkylamines Trimethylamine N-oxide GNE175_Trimethylamine N-oxide (TMAO)_Pos [M+H]+ C3H9NO 75.0684 Organic nitrogen compounds Amine oxides Trisubstituted amine oxides Dopamine GNE176_Dopamine_Pos [M+H]+ C8H11NO2 153.079 Alkaloids Tyrosine alkaloids Phenylethylamines Ala-Ala GNE179_Ala-Ala_Pos [M+H]+ C6H12N2O3 160.0848 Organic acids Amino acids and peptides Dipeptides Ala-His GNE180_Ala-His_Pos [M+H]+ C9H14N4O3 226.1066 Organic acids Amino acids and peptides Dipeptides Ala-Pro GNE181_Ala-Pro_Pos [M+H]+ C8H14N2O3 186.1004 Organic acids Amino acids and peptides Dipeptides Ala-Val/N(2)-Acetyllysine GNE182_Ala-Val_N-Acetyl-L-Lysine_Pos [M+H]+ C8H16N2O3 188.1161 Organic acids Amino acids and peptides Dipeptides Arg-Glu GNE183_Arg-Glu_Pos [M+H]+ C11H21N5O5 303.1543 Organic acids Amino acids and peptides Dipeptides Arg-Leu GNE184_Arg-Leu_Pos [M+H]+ C12H25N5O3 287.1957 Organic acids Amino acids and peptides Dipeptides Arg-Pro GNE185_Arg-Pro_Pos [M+H]+ C11H21N5O3 271.1644 Organic acids Amino acids and peptides Dipeptides Asp-Leu/gamma-Glutamylvaline GNE186_Asp-Leu_Gamma-glutamylvaline_Pos [M+H]+ C10H18N2O5 246.1216 Organic acids Amino acids and peptides Dipeptides Asp-Lys GNE187_Asp-Lys_Pos [M+H]+ C10H19N3O5 261.1325 Organic acids Amino acids and peptides Dipeptides Glu-Cys GNE188_Glu-Cys_Pos [M+H]+ C8H14N2O5S 250.0623 Organic acids Amino acids and peptides Dipeptides Glu-Gln GNE189_Glu-Gln_Pos [M+H]+ C10H17N3O6 275.1117 Organic acids Amino acids and peptides Dipeptides Glu-Glu GNE190_Glu-Glu_Pos [M+H]+ C10H16N2O7 276.0958 Organic acids Amino acids and peptides Dipeptides Glu-Gly GNE191_Glu-Gly_Pos [M+H]+ C7H12N2O5 204.0746 Organic acids Amino acids and peptides Dipeptides Glu-Leu GNE192_Glu-Leu_Neg [M-H]- C11H20N2O5 260.1372 Organic acids Amino acids and peptides Dipeptides Glu-Lys GNE193_Glu-Lys_Pos [M+H]+ C11H21N3O5 275.1481 Organic acids Amino acids and peptides Dipeptides Glu-Thr GNE194_Glu-Thr_Pos [M+H]+ C9H16N2O6 248.1008 Organic acids Amino acids and peptides Dipeptides Gly-Lys GNE195_Gly-Lys_Neg [M-H]- C8H17N3O3 203.127 Organic acids Amino acids and peptides Dipeptides Gly-Val GNE196_Gly-Val_Pos [M+H]+ C7H14N2O3 174.1004 Organic acids Amino acids and peptides Dipeptides Leu-His GNE197_Leu-His_Pos [M+H]+ C12H20N4O3 268.1535 Organic acids Amino acids and peptides Dipeptides Lys-Gln GNE200_Lys-Gln_Pos [M+H]+ C11H22N4O4 274.1641 Organic acids Amino acids and peptides Dipeptides Lys-Leu GNE201_Lys-Leu_Pos [M+H]+ C12H25N3O3 259.1896 Organic acids Amino acids and peptides Dipeptides Lys-Lys GNE202_Lys-Lys_Pos [M+H]+ C12H26N4O3 274.2005 Organic acids Amino acids and peptides Dipeptides Ser-Gln GNE204_Ser-Gln_Pos [M+H]+ C8H15N3O5 233.1012 Organic acids Amino acids and peptides Dipeptides Ser-Val GNE205_Ser-Val_Pos [M+H]+ C8H16N2O4 204.111 Organic acids Amino acids and peptides Dipeptides sn-Glycero-3-phosphate GNE207_Glycerol-3-phosphate_Neg [M-H]- C3H9O6P 172.0137 Organic acids Organic phosphoric acids Organic phosphoric acids Carnitine GNE208_Carnitine_Pos [M+H]+ C7H15NO3 161.1052 Organic nitrogen compounds Carnitines Carnitines CAR 2:0 GNE209_Acetylcarnitine_Pos [M+H]+ C9H17NO4 203.1158 Fatty Acyls Fatty esters Acyl carnitines CAR 3:0 GNE210_Propionylcarnitine_Pos [M+H]+ C10H19NO4 217.1314 Fatty Acyls Fatty esters Acyl carnitines CAR 4:0 GNE211_Butyrylcarnitine_Pos [M+H]+ C11H21NO4 231.1471 Fatty Acyls Fatty esters Acyl carnitines CAR 6:0 GNE212_Hexanoylcarnitine_Pos [M+H]+ C13H25NO4 259.1784 Fatty Acyls Fatty esters Acyl carnitines CAR 14:1 GNE216_Tetradecenoylcarnitine_Pos [M+H]+ C21H39NO4 369.2879 Fatty Acyls Fatty esters Acyl carnitines Azelaic acid GNE228_Azelaic acid_Neg [M-H]- C9H16O4 188.1049 Fatty Acyls Fatty acids Dicarboxylic acids Octadecanedioic acid GNE233_Octadecanedioic acid_Neg [M-H]- C18H34O4 314.2457 Fatty Acyls Fatty acids Dicarboxylic acids 2-Hydroxymyristic acid GNE234_2-Hydroxymyristic acid_Neg [M-H]- C14H28O3 244.2038 Fatty Acyls Fatty acids Hydroxy FA 2-Hydroxypalmitic acid GNE235_2-Hydroxypalmitic acid_Neg [M-H]- C16H32O3 272.2351 Fatty Acyls Fatty acids Hydroxy FA Phosphatidylinositol GNE243_Phosphatidylinositol_Neg [M-H]- C47H83O13P 886.55713 Glycerophospholipids Glycerophosphoinositols PI (Phosphatidylinositols) CDP-Ethanolamine GNE248_CDP-Ethanolamine_Pos [M+H]+ C11H20N4O11P2 446.0604 Nucleic acids Pyrimidines Pyrimidine rNDP Phosphonoacetaldehyde GNE249_Phosphonoacetaldehyde_Pos [M+H]+ C2H5O4P 123.9925 Organic acids Organic phosphonic acids Organic phosphonic acids Phosphoethanolamine/Phosphocolamine GNE250_Phosphorylethanolamine_Phosphocolamine_Pos [M+H]+ C2H8NO4P 141.0191 Organic acids Phosphate esters Phosphoethanolamines Choline GNE252_Choline_Pos [M+H]+ C5H14NO 104.1075 Organic nitrogen compounds Cholines Cholines CDP-choline GNE253_CDP-Choline_Pos [M+H]+ C14H26N4O11P2 488.1073 Nucleic acids Pyrimidines Pyrimidine rNDP Acetylcholine GNE254_Acetylcholine_Pos [M+H]+ C7H16NO2 146.1181 Organic nitrogen compounds Cholines Acyl cholines Choline phosphate GNE255_Phosphorylcholine_Pos [M+H]+ C5H14NO4P 183.066 Organic nitrogen compounds Cholines Cholines Phosphatidylcholine/Lecithin GNE256_Phosphatidylcholine_Lecithin_Pos [M+H]+ - - - - - sn-Glycero-3-phosphocholine GNE257_Glycero-3-phosphocholine_Pos [M+H]+ C8H20NO6P 257.1028 Organic acids Organic phosphoric acids Organic phosphoric acids LPC 18:1/0:0 GNE258_1-Oleoylglycerophosphocholine_Pos [M+H]+ C26H52NO7P 521.3481 Glycerophospholipids Glycerophosphocholines LPC LPC 18:2 GNE259_1-Linoleoylglycerophosphocholine_Pos [M+H]+ C26H50NO7P 519.3325 Glycerophospholipids Glycerophosphocholines LPC Thiamine GNE262_Thiamine (Vitamin B1)_Pos [M+H]+ C12H17N4OS 265.1123 Nucleic acids Pyrimidines Thiamines Pantothenic acid GNE265_Pantothenic acid (Vitamin B5)_Pos [M+H]+ C9H17NO5 219.1107 Organic acids Amino acids and peptides Amino acids Biotin GNE267_Biotin (Vitamin B8)_Pos [M+H]+ C10H16N2O3S 244.0882 Organoheterocyclic compounds Heterocyclic compounds Biotins 4a-Carbinolamine tetrahydrobiopterin GNE271_Dihydrobiopterin_Neg [M-H]- C9H13N5O3 239.1018 Organoheterocyclic compounds Pterins Biopterins Sapropterin GNE272_Tetrahydrobiopterin_Pos [M+H]+ C9H15N5O3 241.1175 Organoheterocyclic compounds Pterins Biopterins Nicotinamide riboside GNE276_Nicotinamide riboside_Neg [M-H]- C11H15N2O5 255.0981 Alkaloids Pyridine alkaloids Nicotinic acid alkaloids N-Methylnicotinamide GNE277_N-Methylnicotinamide_Pos [M+H]+ C7H8N2O 136.0637 Alkaloids Pyridine alkaloids Nicotinic acid alkaloids NAD GNE278_Nicotinamide adenine dinucleotide_Pos [M+H]+ C21H27N7O14P2 663.1091 Nucleic acids Nicotinamides Nicotinamide dinucleotides NADP GNE279_Nicotinamide adenine dinucleotide phosphate_Pos [M+H]+ C21H28N7O17P3 743.0755 Nucleic acids Nicotinamides Nicotinamide dinucleotides 1-Methyladenosine GNE282_1-Methyladenosine_Pos [M+H]+ C11H15N5O4 281.1124 Nucleic acids Purines Purine ribonucleosides 1-Methylguanosine GNE284_1-Methylguanosine_Pos [M+H]+ C11H15N5O5 297.1073 Nucleic acids Purines Purine ribonucleosides 1-Methylxanthine GNE285_1-Methylxanthine_Pos [M+H]+ C6H6N4O2 166.0491 Nucleic acids Purines Xanthines Dimethylxanthine GNE289_Dimethylxanthine_Neg [M-H]- C7H8N4O2 180.0647 Nucleic acids Purines Xanthines Dimethyluric acid GNE290_Dimethyluric acid_Neg [M-H]- C7H8N4O3 196.0596 Nucleic acids Purines Xanthines Betaine GNE291_Betaine_Pos [M+H]+ C5H11NO2 117.079 Organic acids Amino acids and peptides Amino acids Cystine GNE292_Cystine_Pos [M+H]+ C6H12N2O4S2 240.0239 Organic acids Amino acids and peptides Amino acids Cystathionine GNE295_Cystathionine_Pos [M+H]+ C7H14N2O4S 222.0674 Organic acids Amino acids and peptides Amino acids Taurine GNE296_Taurine_Neg [M-H]- C2H7NO3S 125.0147 Organic acids Sulfonic acids Sulfonic acids N(5)-Ethyl-glutamine GNE297_Theanine_Pos [M+H]+ C7H14N2O3 174.1004 Organic acids Amino acids and peptides Amino acids Glutathione GNE298_Glutathione_Pos [M+H]+ C10H17N3O6S 307.0838 Organic acids Amino acids and peptides Tripeptides Homoarginine GNE300_Homoarginine_N-Methylarginine_Pos [M+H]+ C7H16N4O2 188.1273 Organic acids Amino acids and peptides Amino acids Methionine sulfoxide/Targinine GNE303_Methionine sulfoxide_Pos [M+H]+ C5H11NO3S 165.046 Organic acids Amino acids and peptides Amino acids 2-amino-3-methanesulfinylpropanoic acid GNE304_Methylcysteine sulfoxide_Pos [M+H]+ C4H9NO3S 151.03031 Organic acids Amino acids and peptides Amino acids S-Adenosylmethionine GNE305_S-Adenosyl methionine_Pos [M+H]+ C15H23N6O5S 399.1451 Nucleic acids Purines Other purines S-Adenosylhomocysteine GNE306_S-Adenosyl homocysteine_Pos [M+H]+ C14H20N6O5S 384.1216 Nucleic acids Purines Other purines gamma-Aminobutyric acid GNE307_Gamma-Aminobutyric acid_Pos [M+H]+ C4H9NO2 103.0633 Fatty Acyls Fatty acids Amino FA N-Acetylarginine GNE308_N-Acetylarginine_Pos [M+H]+ C8H16N4O3 216.1222 Organic acids Amino acids and peptides Amino acids N-Acetylaspartic acid GNE309_N-Acetylaspartate_Neg [M-H]- C6H9NO5 175.0481 Organic acids Amino acids and peptides Amino acids N-Acetylglutamine GNE314_N-Acetylglutamine_Pos [M+H]+ C7H12N2O4 188.0797 Organic acids Amino acids and peptides Amino acids N-Acetylglutamic acid GNE315_N-Acetylglutamate_Neg [M-H]- C7H11NO5 189.0637 Organic acids Amino acids and peptides Amino acids N-Acetylserine GNE316_N-Acetylserine_Pos [M+H]+ C5H9NO4 147.0532 Organic acids Amino acids and peptides Amino acids N-Acetylleucine GNE317_N-Acetylleucine_Pos [M+H]+ C8H15NO3 173.1052 Organic acids Amino acids and peptides Amino acids N-Acetylhistidine GNE318_N-Acetylhistidine_Pos [M+H]+ C8H11N3O3 197.08 Organic acids Amino acids and peptides Amino acids N(6)-Methyllysine GNE320_N-Methyllysine_Pos [M+H]+ C7H16N2O2 160.1212 Organic acids Amino acids and peptides Amino acids N-Methyl-glutamic acid GNE322_N-Methylglutamate_Pos [M+H]+ C6H11NO4 161.0688 Organic acids Amino acids and peptides Amino acids N-Methylhistidine GNE323_N-Methylhistidine_Pos [M+H]+ C7H11N3O2 169.0851 Organic acids Amino acids and peptides Amino acids N-Methylphenylalanine GNE324_N-Methylphenylalanine_Pos [M+H]+ C10H13NO2 179.09463 Organic acids Amino acids and peptides Amino acids Dimethylarginine GNE325_N,N-Dimethylarginine_Pos [M+H]+ C8H18N4O2 202.143 Organic acids Amino acids and peptides Amino acids N(6),N(6)-Dimethyl-lysine GNE326_N,N-Dimethyllysine_Pos [M+H]+ C8H18N2O2 174.1368 Organic acids Amino acids and peptides Amino acids N-6-Trimethyllysine GNE327_N6,N6,N6-Trimethyl-L-lysine_Pos [M+H]+ C9H20N2O2 188.1525 Organic acids Amino acids and peptides Amino acids N8-Acetylspermidine GNE328_N8-Acetylspermidine_Pos [M+H]+ C9H21N3O 187.1685 Alkaloids Ornithine alkaloids Polyamines Acrylic acid/Pyruvaldehyde GNE331_Acrylic acid_Pyruvaldehyde_Neg [M-H]- C3H4O2 72.0211 Fatty Acyls Fatty acids Unsaturated FA/Other carbonyl compounds Adipic acid/2-Methylglutaric acid GNE332_Adipic acid_2-Methylglutaric acid_Neg [M-H]- C6H10O4 146.0579 Fatty Acyls Fatty acids Dicarboxylic acids Cysteic acid GNE333_Cysteic acid_Neg [M-H]- C3H7NO5S 169.0045 Organic acids Amino acids and peptides Amino acids Crotonic acid GNE334_Crotonic acid_Pos [M+H]+ C4H6O2 86.0368 Fatty Acyls Fatty acids Unsaturated FA Citraconic acid GNE336_Citraconic acid_Pos [M+H]+ C5H6O4 130.0266 Fatty Acyls Fatty acids Dicarboxylic acids Citramalic acid GNE337_Citramalic acid_Neg [M-H]- C5H8O5 148.0372 Organic acids Hydroxy acids Beta hydroxy acids Glycolic acid GNE340_Glycolic acid_Neg [M-H]- C2H4O3 76.016 Fatty Acyls Fatty acids Hydroxy FA Glyceric acid GNE341_Glyceric acid_Neg [M-H]- C3H6O4 106.0266 Carbohydrates Monosaccharides Sugar acids Galactaric acid GNE343_Mucic acid_Neg [M-H]- C6H10O8 210.0376 Fatty Acyls Fatty acids Dicarboxylic acids Pipecolic acid GNE349_Pipecolic acid_Pos [M+H]+ C6H11NO2 129.079 Organic acids Amino acids and peptides Amino acids Quinic acid GNE350_Quinic acid_Neg [M-H]- C7H12O6 192.0634 Benzenoids Benzenes Hydroxybenzoic acids Salicylic acid GNE351_Salicylic acid_Neg [M-H]- C7H6O3 138.0317 Benzenoids Benzenes Hydroxybenzoic acids Pyroglutamic acid GNE355_5-Oxoproline_Pos [M+H]+ C5H7NO3 129.0426 Organic acids Amino acids and peptides Amino acids 3-Methyl pyruvic acid GNE358_2-Ketobutyric acid_Neg [M-H]- C4H6O3 102.0317 Fatty Acyls Fatty acids Oxo FA 3-Hydroxyanthranilic acid GNE365_3-Hydroxyanthranilic acid_Neg [M-H]- C7H7NO3 153.0426 Benzenoids Benzenes Hydroxybenzoic acids cis-4-Hydroxyproline GNE366_4-Hydroxyproline_Pos [M+H]+ C5H9NO3 131.0582 Organic acids Amino acids and peptides Amino acids 2-Hydroxy-3-methylbutyric acid GNE369_2-Hydroxy-3-Methylbutyric acid_Neg [M-H]- C5H10O3 118.063 Fatty Acyls Fatty acids Hydroxy FA 4-Imidazolone-5-propionic acid/Imidazolelactic acid GNE375_4-Imidazolone-5-propanoate_Imidazolelactic acid_Pos [M+H]+ C6H8N2O3 156.0535 Alkaloids Histidine alkaloids Imidazole alkaloids 4-Acetamidobutanoic acid GNE376_4-Acetamidobutanoic acid_Pos [M+H]+ C6H11NO3 145.0739 Organic acids Amino acids and peptides Amino acids 5-Aminopentanoic acid GNE377_5-Aminovalerate_Pos [M+H]+ C5H11NO2 117.079 Fatty Acyls Fatty acids Amino FA 4-Hydroxybenzoic acid/Dihydroxybenzaldehyde GNE380_4-Hydroxybenzoic acid_Dihydroxybenzaldehyde_Neg [M-H]- C7H6O3 138.0317 Benzenoids Benzenes Hydroxybenzoic acids 4-Hydroxybenzaldehyde GNE381_4-Hydroxybenzaldehyde_Pos [M+H]+ C7H6O2 122.0368 Benzenoids Benzenes Hydroxybenzoic acids DOPA GNE384_Dihydroxyphenylalanine_Pos [M+H]+ C9H11NO4 197.0688 Organic acids Amino acids and peptides Amino acids Methylimidazoleacetic acid GNE388_Methylimidazole acetic acid_Pos [M+H]+ C6H8N2O2 140.0586 Alkaloids Histidine alkaloids Imidazole alkaloids Benzoic acid GNE392_Benzoic acid_Pos [M+H]+ C7H6O2 122.0368 Benzenoids Benzenes Hydroxybenzoic acids Imidazole GNE398_Imidazole_Pos [M+H]+ C3H4N2 68.0374 Alkaloids Histidine alkaloids Imidazole alkaloids Phenol GNE401_Phenol_Neg [M-H]- C6H6O 94.0419 Benzenoids Benzenes Hydroxybenzoic acids Toluene GNE407_Toluene_Pos [M+H]+ C7H8 92.0626 Benzenoids Benzenes Toluenes Xylene GNE408_Xylene_Pos [M+H]+ C8H10 106.07825 Benzenoids Benzenes Benzenes Asp-Glu GNE409_Asp-Glu_Pos [M+H]+ C9H14N2O7 262.0801 Organic acids Amino acids and peptides Dipeptides Argininosuccinic acid GNE420_Argininosuccinic acid_Pos [M+H]+ C10H18N4O6 290.1226 Organic acids Amino acids and peptides Amino acids Isovalerylglycine GNE421_Isovalerylglycine_Neg [M-H]- C7H13NO3 159.0895 Organic acids Amino acids and peptides Amino acids O-Acetyl-homoserine GNE426_O-Acetyl-L-Homoserine_Pos [M+H]+ C6H11NO4 161.0688 Organic acids Amino acids and peptides Amino acids N-Acetylmethionine GNE429_N-Acetyl-L-Methionine_Neg [M-H]- C7H13NO3S 191.0616 Organic acids Amino acids and peptides Amino acids N-Acetylputrescine GNE431_N-Acetylputrescine_Pos [M+H]+ C6H14N2O 130.1106 Alkaloids Ornithine alkaloids Polyamines Sphingosine GNE432_Sphingosine_Pos [M+H]+ C18H37NO2 299.2824 Sphingolipids Sphingoid bases Sphingosines Pseudouridine GNE433_Pseudouridine_Neg [M-H]- C9H12N2O6 244.0695 Nucleic acids Pyrimidines Pyrimidine ribonucleosides 4-Guanidinobutanoic acid GNE438_4-Guanidinobutanoate_Pos [M+H]+ C5H11N3O2 145.0851 Organic nitrogen compounds Guanidines Guanidines 3-Methyl-2-oxovaleric acid GNE444_3-Methyl-2-oxovalerate_Neg [M-H]- C6H10O3 130.063 Organic acids Keto acids Short-chain keto acids Dihydrouracil GNE450_5,6-Dihydrouracil_Neg [M-H]- C4H6N2O2 114.0429 Nucleic acids Pyrimidines Other pyrimidines 5'-Methylthioadenosine GNE452_5-Methylthioadenosine (MTA)_Pos [M+H]+ C11H15N5O3S 297.0896 Nucleic acids Purines Purine ribonucleosides Acisoga GNE454_Acisoga_Pos [M+H]+ C9H16N2O2 184.1212 Alkaloids Ornithine alkaloids Pyrrolidine alkaloids Allantoin GNE455_Allantoin_Neg [M-H]- C4H6N4O3 158.044 Organoheterocyclic compounds Ureas Ureides Dimethylglycine GNE462_Dimethylglycine_Pos [M+H]+ C4H9NO2 103.0633 Organic acids Amino acids and peptides Amino acids gamma-Glutamylalanine GNE463_Gamma-glutamylalanine_Pos [M+H]+ C8H14N2O5 218.0903 Organic acids Amino acids and peptides Dipeptides gamma-Glutamylhistidine GNE466_Gamma-glutamylhistidine_Neg [M-H]- C11H16N4O5 284.1121 Organic acids Amino acids and peptides Dipeptides gamma-Glutamylmethionine GNE468_Gamma-glutamylmethionine_Pos [M+H]+ C10H18N2O5S 278.0936 Organic acids Amino acids and peptides Dipeptides Guanidoacetic acid GNE472_Guanidinoacetate_Pos [M+H]+ C3H7N3O2 117.0538 Organic acids Amino acids and peptides Amino acids Guanidinosuccinic acid GNE473_Guanidinosuccinate_Pos [M+H]+ C5H9N3O4 175.0593 Organic nitrogen compounds Guanidines Guanidines Homocitrulline GNE474_Homocitrulline_Pos [M+H]+ C7H15N3O3 189.1113 Organic acids Amino acids and peptides Amino acids CAR 4:0;3Me GNE476_Isovalerylcarnitine (C5)_Pos [M+H]+ C12H23NO4 245.1627 Fatty Acyls Fatty esters Acyl carnitines Leucylglycine/N(2)-Acetyllysine GNE477_Leucylglycine/N-acetyllysine_Pos [M+H]+ C8H16N2O3 188.1161 Organic acids Amino acids and peptides Amino acids Methylsuccinic acid GNE481_Methylsuccinate_Neg [M-H]- C5H8O4 132.0423 Fatty Acyls Fatty acids Dicarboxylic acids N-Acetylneuraminic acid GNE486_N-acetylneuraminate_Neg [M-H]- C11H19NO9 309.106 Carbohydrates Monosaccharides Sugar acids N-Acetyltaurine GNE489_N-acetyltaurine_Neg [M-H]- C4H9NO4S 167.0252 Organic acids Organosulfonic acids Organosulfonic acids N-Acetyl-tryptophan GNE490_N-acetyltryptophan_Neg [M-H]- C13H14N2O3 246.1004 Organic acids Amino acids and peptides Amino acids N(5)-Acetyl-ornithine GNE493_N-delta-acetylornithine_Pos [M+H]+ C7H14N2O3 174.1004 Organic acids Amino acids and peptides Amino acids O-cresol sulfate/P-cresol sulfate GNE495_O-cresol sulfate/P-cresol sulfate_Neg [M-H]- C7H8O4S 188.01433 Organic acids Arylsulfates Phenylsulfates Orotidine GNE496_Orotidine_Neg [M-H]- C10H12N2O8 288.0594 Nucleic acids Pyrimidines Pyrimidine ribonucleosides Phenyl hydrogen sulfate GNE497_Phenol sulfate_Neg [M-H]- C6H6O4S 173.9987 Benzenoids Benzenes Hydroxybenzoic acids Phenylacetylglycine GNE498_Phenylacetylglycine_Neg [M-H]- C10H11NO3 193.0739 Organic acids Amino acids and peptides Amino acids Pro-hydroxyPro GNE499_Prolylhydroxyproline_Pos [M+H]+ C10H16N2O4 228.111 Organic acids Amino acids and peptides Dipeptides 1-Pyrroline-5-carboxylic acid GNE502_S-1-pyrroline-5-carboxylate_Neg [M-H]- C5H7NO2 113.0477 Organoheterocyclic compounds Pyrrolines Pyrrolines Sucrose/Maltose GNE503_Sucrose_Maltose_Neg [M-H]- C12H22O11 342.1162 Carbohydrates Disaccharides Disaccharides Urocanic acid GNE505_Trans-urocanate_Pos [M+H]+ C6H6N2O2 138.0429 Alkaloids Histidine alkaloids Imidazole alkaloids Ala-Leu GNE517_Alanylleucine_Pos [M+H]+ C9H18N2O3 202.1317 Organic acids Amino acids and peptides Dipeptides N-Carboxyethyl-gamma-aminobutyric acid GNE519_Carboxyethyl-GABA_Pos [M+H]+ C7H13NO4 175.0845 Fatty Acyls Fatty acids Amino FA FMN GNE521_Flavin mononucleotide (FMN)_Neg [M-H]- C17H21N4O9P 456.1046 Organoheterocyclic compounds Alloxazines and isoalloxazines Flavins Leu-Gln GNE524_Leucylglutamine_Pos [M+H]+ C11H21N3O4 259.1532 Organic acids Amino acids and peptides Dipeptides Methanolphosphate GNE526_Methylphosphate_Neg [M-H]- CH5O4P 111.9925 Organic acids Phosphate esters Monoalkyl phosphates 6-Dimethylaminopurine GNE528_N6-dimethylallyladenine_Pos [M+H]+ C7H9N5 163.0858 Nucleic acids Purines Alkylaminopurines Aspartylglycosamine GNE530_N-acetylglucosaminylasparagine _Neg [M-H]- C12H21N3O8 335.1329 Organic acids Amino acids and peptides Amino acids Pro-Gly GNE540_Prolylglycine_Pos [M+H]+ C7H12N2O3 172.0848 Organic acids Amino acids and peptides Dipeptides Pyridoxal GNE541_Pyridoxal_Pos [M+H]+ C8H9NO3 167.0582 Alkaloids Pyridine alkaloids Nicotinic acid alkaloids Cinnamoylglycine GNE548_Cinnamoylglycine_Neg [M-H]- C11H11NO3 205.0739 Organic acids Phenylpropanoids Cinnamic acids Hippuric acid GNE550_Hippurate_Neg [M-H]- C9H9NO3 179.0582 Benzenoids Benzamides Hippuric acids METABOLITES_END #END