#METABOLOMICS WORKBENCH renpingl_20250107_141746 DATATRACK_ID:5510 STUDY_ID:ST003695 ANALYSIS_ID:AN006062 PROJECT_ID:PR002293 VERSION 1 CREATED_ON January 14, 2025, 6:52 pm #PROJECT PR:PROJECT_TITLE Investigating the role of 4-diets through multi-organ and multi-omic crosstalk PR:PROJECT_TYPE Targeted MS quantitative analysis PR:PROJECT_SUMMARY Presently, dietary patterns have undergone significant shifts, and understanding PR:PROJECT_SUMMARY the intricate interplay between diet, particularly high fat and high sucrose PR:PROJECT_SUMMARY diets, the gut microbiome, and cardiometabolic health has become paramount. PR:PROJECT_SUMMARY These dietary patterns have been consistently associated with heightened PR:PROJECT_SUMMARY cardiometabolic risk factors including obesity, insulin resistance, PR:PROJECT_SUMMARY dyslipidemia, and hypertension. High-fat diets, in particular, contribute to PR:PROJECT_SUMMARY increased adiposity and ectopic fat deposition, exacerbating systemic PR:PROJECT_SUMMARY inflammation and oxidative stress, thereby promoting the development of PR:PROJECT_SUMMARY metabolic dysfunction. Similarly, high sucrose diets have been implicated in the PR:PROJECT_SUMMARY dysregulation of glucose homeostasis, leading to insulin resistance and PR:PROJECT_SUMMARY hyperglycemia, key hallmarks of cardiometabolic diseases such as type 2 diabetes PR:PROJECT_SUMMARY mellitus. Amidst this, the exploration of multi-omic profiles alongside the gut PR:PROJECT_SUMMARY microbial landscape has emerged as a pivotal avenue for unraveling the PR:PROJECT_SUMMARY complexities of cardiometabolic health dynamics affected by the effects of PR:PROJECT_SUMMARY high-fat and high-sucrose diets. The approach of using a multi-omic comparison PR:PROJECT_SUMMARY between organs offers a comprehensive lens through which the intricate molecular PR:PROJECT_SUMMARY signatures underlying the impact of dietary compositions, particularly high fat PR:PROJECT_SUMMARY and high sucrose, on metabolic health, can be examined. PR:INSTITUTE University of Sydney PR:DEPARTMENT School of Medical Sciences PR:LABORATORY Cardiometabolic Disease PR:LAST_NAME Liu PR:FIRST_NAME Renping PR:ADDRESS The Hub, Charles Perkins Centre, D17, The University of Sydney, NSW, 2006 PR:EMAIL renping.liu@sydney.edu.au PR:PHONE +61432953638 #STUDY ST:STUDY_TITLE Metabolomics studies on mouse cecum samples on a Western diet ST:STUDY_SUMMARY Targeted metabolomics was performed using liquid chromatography-tandem mass ST:STUDY_SUMMARY spectrometry (LC-MS/MS).to measure polar metabolites in both positive and ST:STUDY_SUMMARY negative ionization mode on cecummice tissue acquired after a 30 week dietary ST:STUDY_SUMMARY intervention. ST:INSTITUTE University of Sydney ST:DEPARTMENT School of Medical Sciences ST:LABORATORY Cardiometabolic Disease ST:LAST_NAME Liu ST:FIRST_NAME Renping ST:ADDRESS Charles Perkins Centre ST:EMAIL renping.liu@sydney.edu.au ST:PHONE +61432953638 ST:NUM_GROUPS 4 ST:TOTAL_SUBJECTS 35 ST:NUM_MALES 35 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 SU:GENOTYPE_STRAIN C57BL/6J SU:AGE_OR_AGE_RANGE 6 weeks old SU:GENDER Male SU:ANIMAL_ANIMAL_SUPPLIER Australian Resource Centre SU:ANIMAL_HOUSING 3 per cage SU:ANIMAL_LIGHT_CYCLE 12-hour light-dark cycle #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - C59.1 Sample source:cecum | Diet:chow RAW_FILE_NAME(Raw file name)=C59.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - C59.2 Sample source:cecum | Diet:chow RAW_FILE_NAME(Raw file name)=C59.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - C59.3 Sample source:cecum | Diet:chow RAW_FILE_NAME(Raw file name)=C59.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - C61.1 Sample source:cecum | Diet:chow RAW_FILE_NAME(Raw file name)=C61.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - C61.2 Sample source:cecum | Diet:chow RAW_FILE_NAME(Raw file name)=C61.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - C61.3 Sample source:cecum | Diet:chow RAW_FILE_NAME(Raw file name)=C61.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - C62.1 Sample source:cecum | Diet:chow RAW_FILE_NAME(Raw file name)=C62.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - C62.2 Sample source:cecum | Diet:chow RAW_FILE_NAME(Raw file name)=C62.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - C62.3 Sample source:cecum | Diet:chow RAW_FILE_NAME(Raw file name)=C62.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - HF58.1 Sample source:cecum | Diet:hfd RAW_FILE_NAME(Raw file name)=HF58.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - HF58.2 Sample source:cecum | Diet:hfd RAW_FILE_NAME(Raw file name)=HF58.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - HF58.3 Sample source:cecum | Diet:hfd RAW_FILE_NAME(Raw file name)=HF58.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - HF65.1 Sample source:cecum | Diet:hfd RAW_FILE_NAME(Raw file name)=HF65.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - HF65.2 Sample source:cecum | Diet:hfd RAW_FILE_NAME(Raw file name)=HF65.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - HF65.3 Sample source:cecum | Diet:hfd RAW_FILE_NAME(Raw file name)=HF65.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - HF67.1 Sample source:cecum | Diet:hfd RAW_FILE_NAME(Raw file name)=HF67.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - HF67.2 Sample source:cecum | Diet:hfd RAW_FILE_NAME(Raw file name)=HF67.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - HF67.3 Sample source:cecum | Diet:hfd RAW_FILE_NAME(Raw file name)=HF67.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - HFHS56.1 Sample source:cecum | Diet:hfhsd RAW_FILE_NAME(Raw file name)=HFHS56.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - HFHS56.2 Sample source:cecum | Diet:hfhsd RAW_FILE_NAME(Raw file name)=HFHS56.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - HFHS56.3 Sample source:cecum | Diet:hfhsd RAW_FILE_NAME(Raw file name)=HFHS56.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - HFHS57.1 Sample source:cecum | Diet:hfhsd RAW_FILE_NAME(Raw file name)=HFHS57.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - HFHS57.3 Sample source:cecum | Diet:hfhsd RAW_FILE_NAME(Raw file name)=HFHS57.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - HFHS66.1 Sample source:cecum | Diet:hfhsd RAW_FILE_NAME(Raw file name)=HFHS66.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - HFHS66.2 Sample source:cecum | Diet:hfhsd RAW_FILE_NAME(Raw file name)=HFHS66.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - HFHS66.3 Sample source:cecum | Diet:hfhsd RAW_FILE_NAME(Raw file name)=HFHS66.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - HS60.1 Sample source:cecum | Diet:hsd RAW_FILE_NAME(Raw file name)=HS60.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - HS60.2 Sample source:cecum | Diet:hsd RAW_FILE_NAME(Raw file name)=HS60.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - HS60.3 Sample source:cecum | Diet:hsd RAW_FILE_NAME(Raw file name)=HS60.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - HS63.1 Sample source:cecum | Diet:hsd RAW_FILE_NAME(Raw file name)=HS63.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - HS63.2 Sample source:cecum | Diet:hsd RAW_FILE_NAME(Raw file name)=HS63.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - HS63.3 Sample source:cecum | Diet:hsd RAW_FILE_NAME(Raw file name)=HS63.3_cecum.mzML SUBJECT_SAMPLE_FACTORS - HS64.1 Sample source:cecum | Diet:hsd RAW_FILE_NAME(Raw file name)=HS64.1_cecum.mzML SUBJECT_SAMPLE_FACTORS - HS64.2 Sample source:cecum | Diet:hsd RAW_FILE_NAME(Raw file name)=HS64.2_cecum.mzML SUBJECT_SAMPLE_FACTORS - HS64.3 Sample source:cecum | Diet:hsd RAW_FILE_NAME(Raw file name)=HS64.3_cecum.mzML #COLLECTION CO:COLLECTION_SUMMARY Mouse cecum samples were collected at the end of the experimental design (after CO:COLLECTION_SUMMARY 30-weeks on the diet) during the cull. These samples were immediately clamped CO:COLLECTION_SUMMARY with metal clamps and snap-frozen in liquid nitrogen and stored in -80 degrees CO:COLLECTION_SUMMARY Celsius until further analysis. This process was approved by the University of CO:COLLECTION_SUMMARY Sydney’s Ethics Committee (USYD # 2017/1294). CO:SAMPLE_TYPE Cecum CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY 36 male C57BL/6J mice were allocated to four different diets (n = 7-8) TR:TREATMENT_SUMMARY consisting of a control diet (Chow), a high-sucrose diet (HSD), a high-fat diet TR:TREATMENT_SUMMARY (HFD), and a high-fat high-sucrose (western) diet (HFHSD) at 8 weeks of age. TR:TREATMENT_SUMMARY Mice had ad libitum access to their food and autoclaved water. One mouse was TR:TREATMENT_SUMMARY euthanised prior to the expected experimental endpoint due to the loss of body TR:TREATMENT_SUMMARY condition and was excluded from this study. 4 samples were not collected after TR:TREATMENT_SUMMARY euthanisation making the groups (n = 7-8) TR:TREATMENT Chow, HFD, HSD, HFHSD #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Approximately 50 mg of ground cecum tissue was weighed into 2 mL Eppendorf tube SP:SAMPLEPREP_SUMMARY and subjected to a two-phase extraction protocol involving tissue, ice-cold SP:SAMPLEPREP_SUMMARY extraction medium (methanol:water, and chloroform). Samples were then SP:SAMPLEPREP_SUMMARY centrifuged at 14 000 x g at 4 °C for 25 min. The aqueous layer was transferred SP:SAMPLEPREP_SUMMARY into a new microfuge tube and reconstituted in the acetonitrile/methanol/formic SP:SAMPLEPREP_SUMMARY acid (75:25:0.2; v/v/v, HPLC grade; Thermo Fisher Scientific) for the HILIC SP:SAMPLEPREP_SUMMARY analysis, and acetonitrile/methanol (25:25; v/v/v, HPLC grade) for the AMIDE and SP:SAMPLEPREP_SUMMARY NAD analysis. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY Chromatographic method to detect analytes such as amino acids, endocannabinoids, CH:CHROMATOGRAPHY_SUMMARY fatty acid β-oxidation intermediates, gut-derived metabolites, nucleotides, CH:CHROMATOGRAPHY_SUMMARY neurotransmitters, cofactors and vitamins. CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Agilent 1260 CH:COLUMN_NAME Waters Atlantis T3 (150 x 4.6mm,5um) CH:SOLVENT_A 100% water; 0.1% formic acid; 10 mM ammonium formate (pH ~2.5) CH:SOLVENT_B 100% acetonitrile; 0.1% formic acid CH:FLOW_GRADIENT The LC gradient program begins with an initial condition of 5% solvent A and 95% CH:FLOW_GRADIENT solvent B, with a flow rate of 0.25 mL/min, which is held for 0.5 minutes to CH:FLOW_GRADIENT establish system equilibration. The gradient then proceeds as follows: at 6 CH:FLOW_GRADIENT minutes, the mobile phase composition shifts to 60% solvent A and 40% solvent B CH:FLOW_GRADIENT for 3minutes; at 10 minutes, it changes back to 5% solvent A and 95% solvent B; CH:FLOW_GRADIENT at 11 minutes, the flow rate increases to 0.4 mL/min while maintaining a CH:FLOW_GRADIENT composition of 5% solvent A and 95% solvent B for a duration of 12.5 minutes; CH:FLOW_GRADIENT and at 24.5 minutes, the flow rate decreases back to 0.25 mL/min. The final CH:FLOW_GRADIENT condition is maintained for 1 minutes to ensure stability before subsequent CH:FLOW_GRADIENT analyses. CH:FLOW_RATE 0.250 – 0.400 mL/min CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 6500 QTrap MS:INSTRUMENT_TYPE QTRAP MS:MS_TYPE API MS:ION_MODE POSITIVE MS:MS_COMMENTS Targeted metabolite profiling was performed using MS multiple MS:MS_COMMENTS reaction-monitoring transitions. Multiple Reaction Monitoring (MRM) Q1/Q3 peak MS:MS_COMMENTS integration of the raw data files (Analyst software, v.1.6.2; ABSciex) was MS:MS_COMMENTS performed using software MultiQuant 3.0 (ABSciex). #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak Area MS_METABOLITE_DATA_START Samples C59.1 C59.2 C59.3 C61.1 C61.2 C61.3 C62.1 C62.2 C62.3 HF58.1 HF58.2 HF58.3 HF65.1 HF65.2 HF65.3 HF67.1 HF67.2 HF67.3 HFHS56.1 HFHS56.2 HFHS56.3 HFHS57.1 HFHS57.3 HFHS66.1 HFHS66.2 HFHS66.3 HS60.1 HS60.2 HS60.3 HS63.1 HS63.2 HS63.3 HS64.1 HS64.2 HS64.3 Factors Sample source:cecum | Diet:chow Sample source:cecum | Diet:chow Sample source:cecum | Diet:chow Sample source:cecum | Diet:chow Sample source:cecum | Diet:chow Sample source:cecum | Diet:chow Sample source:cecum | Diet:chow Sample source:cecum | Diet:chow Sample source:cecum | Diet:chow Sample source:cecum | Diet:hfd Sample source:cecum | Diet:hfd Sample source:cecum | Diet:hfd Sample source:cecum | Diet:hfd Sample source:cecum | Diet:hfd Sample source:cecum | Diet:hfd Sample source:cecum | Diet:hfd Sample source:cecum | Diet:hfd Sample source:cecum | Diet:hfd Sample source:cecum | Diet:hfhsd Sample source:cecum | Diet:hfhsd Sample source:cecum | Diet:hfhsd Sample source:cecum | Diet:hfhsd Sample source:cecum | Diet:hfhsd Sample source:cecum | Diet:hfhsd Sample source:cecum | Diet:hfhsd Sample source:cecum | Diet:hfhsd Sample source:cecum | Diet:hsd Sample source:cecum | Diet:hsd Sample source:cecum | Diet:hsd Sample source:cecum | Diet:hsd Sample source:cecum | Diet:hsd Sample source:cecum | Diet:hsd Sample source:cecum | Diet:hsd Sample source:cecum | Diet:hsd Sample source:cecum | Diet:hsd 1-methylhistamine 42124890.36 37822641.93 40965048.74 75219236.76 43400624.12 37089067.19 41802787.04 150859872.6 51240884.9 35557844.65 44015847.09 37654734.43 44001089.79 43861760.26 37503684.8 48038816.26 41385811.21 44993574.13 46982142.1 39223712.04 37232966.08 40092765.47 51020238.38 60823431.38 41479763.01 59914517.53 58774467.72 73868220.89 42106122.16 60132542.26 74134487.28 48527250.7 50274822.98 46680324.84 89699565.86 2-Arachidonyl glycerol 694915.142 586239.8565 580165.8935 380520.18 396442.3524 390001.207 156616.5523 293654.7145 341051.5105 650352.7785 505230.281 588760.2835 507205.0005 390693.429 532704.827 614224.8325 564482.3625 500031.6792 790362.773 588667.417 371826.379 692802.5515 636518.0879 719269.8855 603725.4095 500761.658 453042.463 442291.745 407574.0988 548790.9145 330793.0365 280505.043 367089.0785 407151.2825 676880.0148 2'-deoxyadenosine 12408.263 33252.112 30823.591 33286.112 21990.7455 18195.651 15527.1035 18326.927 19836.9405 23122.8805 28870.6925 25030.6955 15746.1215 18500.3955 32132.5295 31825.4645 34137.305 25975.913 29519.6825 29143.596 26442.396 36802.952 27716.486 36221.4915 23451.394 25394.501 21489.251 12589.2925 21915.164 30069.741 11909.77 11782.704 26989.9405 20942.4735 32426.6345 2'-deoxycytidine 735406.8305 2763244.029 930745.42 2922928.988 2328843.373 3700907.337 2268760.801 4438775.523 2359433.106 1573909.759 2708865.418 1186399.384 1520928.663 3985171.603 2166220.101 1652922.483 2895157.86 6283004.414 858356.569 1092114.029 1339747.578 887926.1715 1065544.161 785019.723 1782847.825 1292167.704 806465.7175 1842966.518 1217642.565 1859862.378 730737.7658 1450360.697 1822064.444 2089526.171 2672881.258 2-Methylbutyrylcarnitine.1 101134.556 312036.1045 63478.47927 51431.1125 254611.4685 51173.403 98702.6965 92535.909 933669.522 2109170.291 230123.1185 58887.888 52098.457 192224.811 148084.461 123533.6215 79695.019 73744.08749 1383169.794 100215.7535 258276.4734 184215.072 372348.2958 452481.3785 127651.865 476625.994 445624.5966 154413.9335 1733444.026 80998.886 67193.6575 49757.0165 2864922.246 128199.6645 179414.259 2-Methylbutyrylcarnitine.2 116289.7983 217831.4496 218159.5484 179575.4727 143811.2408 120825.4583 144744.567 102366.5208 559500.0912 1267235.993 481277.0223 132326.6477 184345.5 509737.1715 322570.2945 323765.8838 444093.36 395418.8766 824815.911 609129.6795 319643.0924 390376.2407 1220958.338 442023.8096 1325895.052 475026.9587 406191.2155 270859.2065 1073923.022 248904.5778 455930.1591 278040.2674 1654608.048 234676.0195 788299.1477 2-Methylbutyrylcarnitine.3 795540.5671 1139609.246 1276394.457 1530139.488 1956520.787 1439808.104 1664952.566 1755660.873 690735.2402 577482.2085 1245982.126 777213.8712 1804644.083 1576027.313 1513393.235 700114.5481 932391.0372 2851557.259 954005.5864 994627.1686 1866547.331 764125.1814 1576400.803 1061794.538 1113105.095 1178693.505 767986.3587 362246.9964 722649.2289 550845.3689 1658997.953 1660133.047 1071374.163 933789.8085 1106537.588 3-deaazadenosine 80548.00082 240777.4005 283774.19 612267.153 452176.8415 615235.8205 403722.9765 648666.9879 470222.762 639427.368 543918.8265 263962.4805 440245.372 856088.912 611490.4025 530587.675 678029.6465 734350.2015 375356.638 425545.456 516038.369 424824.0455 368290.6145 316496.764 494333.248 429744.0375 256387.6115 733985.906 466202.4935 409412.245 374657.3345 418328.801 549922.6236 469433.496 517336.1955 3HK 35561.6535 19665.5425 41448.3245 35186.593 27425.9515 37543.82928 15313.857 10711.051 25219.0495 19933.31 32213.974 34091.979 22455.668 36040.8895 29442.9945 26244.3325 21150.5775 33885.1025 24106.5105 91862.7825 16330.4475 35961.7605 27183.5205 112152.508 37797.8865 24713.523 30363.4305 37526.044 16732.8275 55385.9875 32203.171 28789.5375 19786.5755 23669.914 19235.781 3-IPA 301325.253 1403055.172 879706.26 1308501.192 967137.5885 2858493.863 1334692.177 1890547.039 687265.568 510096.713 1584642.042 599302.6135 1608912.568 2132246.996 2134841.104 657362.7445 981842.0675 1307590.008 458894.3875 375668.326 144777.5165 627664.9445 856538.4845 285910.3345 489227.0745 812298.849 457173.933 274155.497 290791.487 464374.912 603633.5035 456546.815 765097.839 1018759.783 303582.877 5-Aminolevulinic Acid 143037.454 141552.1165 48659.2325 64901.6285 129410.257 26459.755 24407.914 35357.749 281587.2595 428734.1405 121848.817 31034.918 38472.814 163811.3505 86543.71 83910.36 63976.4815 35236.4945 420901.4045 61796.4685 159845.5685 104305.4005 243148.769 208631.8625 29713.504 148759.293 207914.9255 74987.6805 416768.487 85193.515 58773.9515 137761.9515 494864.9517 71529.547 80609.161 5-HIAA 51951.86363 83700.3395 91277.01876 56044.26555 95421.43766 56781.43913 173449.4485 97335.3178 74927.4795 66178.50578 86370.06307 62059.08791 58049.4795 86690.00453 73012.90436 57082.05927 106697.7649 61445.2968 78967.7285 102554.9263 79899.2915 92877.94637 79320.127 96164.87 101629.5949 101821.4965 51106.648 54811.09318 33274.92264 36146.266 42528.72893 56192.54 219711.061 77583.50464 72494.10204 AAD 197636.541 488315.1995 777993.638 649299.877 543536.9695 604982.6465 894702.16 182440.449 882110.5885 397398.1975 910208.857 678281.98 382735.5165 623721.5635 415592.9065 822171.235 460969.988 243427.6695 492179.9215 604758.0505 388625.657 738134.418 710508.8235 1945406.489 1362627.119 328885.8555 572658.106 364044.418 687533.637 1710702.998 81148.816 1277945.943 465076.393 893444.1655 636664.802 Acetoacetic Acid 335801.4095 305935.2685 245604.313 307567.5945 330652.6185 287536.28 285010.6045 299976.5835 343272.6535 824060.9385 241255.0635 324947.988 289897.79 247250.07 256526.798 303964.283 280295.2125 297581.239 736191.0215 238578.2905 288459.524 280364.8665 236423.761 261750.319 229140.697 313928.5165 265853.2425 324136.487 557553.7735 297029.117 293773.3265 311521.119 744406.685 269104 233203.446 Acetylcarnitine 28956035.61 56891157.73 4914696.456 16819285.52 85069792.74 15958509.11 11021139.81 25380338.74 138633094.2 146897925.2 33738536.56 8309200.394 16527643.59 87497988.03 23436204.41 26548129.73 11618122.03 10260047.29 184305604.2 7147330.776 106180708.5 34867500.17 50344985.08 65914154.18 4136586.118 35398290.01 87440648.79 21234628.43 286082449.9 19585525.01 15771307.2 32814395.69 141182533.6 26296685.27 46339296.26 Acetylcholine 40253727.44 51750280.12 54621525.34 25611732.62 24323535.5 19617133.46 20023792.68 13566647.03 22286960.26 39638386.36 40504896.29 43467744.5 22322790.39 21636560.55 39329220.9 42545764.95 32703181.28 17144834.7 51954768.79 53340383.76 25256595.2 56133698.07 33512690.86 63156598.3 56598721.88 28114237.63 31416332.79 39114396.46 23275079.7 45589630.41 27773315.31 28190756.18 16995534.26 30890742.17 23818693.09 Adenosine 840377461.3 1593395351 638108613.9 1677686358 1590210077 1919900158 1461083118 1998521786 2228514455 1516401622 1309621940 1053531915 1199000631 1741682156 1005174758 905334148 1392740253 2031225921 781255362.1 385044121.7 846881968.1 626492948.5 526485462 557247639 612600482.6 482349558.2 427195513.4 1478579463 949229181.6 1393296268 635291289.1 744210539.8 1539427587 1040924296 726089251.9 ADMA 16447806.47 27330605.38 17553357.11 6559438.483 18986469.16 23513736.09 21817856.06 5160311.591 8502556.824 48779441.45 22435601.84 14906665.91 16826634.46 16856820.04 26128454.16 20402915.07 37207449.24 23713997.82 31391912.34 31425461.35 23444749.77 21365605.83 21096479.45 21106636.47 31415247.22 17459855.41 17265620.82 5342024.481 10142269.67 6299055.771 4221826.3 4567346.944 9536993.216 9132894.566 4512479.772 Alanine 8618785.81 11071452.18 10309645.36 7928588.193 9393406.83 5880727.788 8246491.615 7999944.087 14770289.01 22078922.79 21960723.1 6646631.253 8917689.642 13353766.53 11286243.91 11088540.64 12847685.71 22217386.95 19585538.33 19820345.2 16704238.57 16518319.88 26594522.42 18652314.34 21409882.21 22750849.65 21459601.75 6612206.007 15889545.62 7275638.771 12501998.23 7969768.165 31264238.92 8460280.761 14252105.27 Anandamide 266807.3458 247551.717 283958.47 254834.6793 253644.067 281893.8325 241618.9435 198612.9109 211661.518 228803.3015 309817.5028 228893.514 205360.6895 198293.6365 260261.085 235391.3015 217764.9755 207483.0979 257047.7182 289480.1405 239773.7915 308821.297 280468.6425 234317.693 236897.7775 200279.7761 244058.9305 229588.586 245197.1057 258732.6367 252508.282 197448.8289 145582.5865 173642.3202 295887.088 Anserine 1832537.846 1055918.454 169044.873 90910.1715 231502.74 153693.189 89875.4445 348499.574 283044.67 638884.275 221665.8828 68606.5855 72423.91591 445677.4916 96670.9155 203073.1165 147709.128 255611.884 1190338.843 1026827.799 629018.3844 702861.8685 891326.5472 187273.5585 54986.8165 192940.395 1553430.289 1084499.275 1031354.462 166514.9435 181646.4445 521322.5346 990114.2175 244547.5375 542375.894 Arachidonic acid 15895550.12 27289193.37 27722079.04 13122532.1 13958384.68 13311210.81 9223241.066 10167760.34 12741918.13 20878691.19 29918500.26 16449239.12 14032440.99 11233386.38 18243889.32 21234330.62 17490472.6 8998362.284 28452292.26 23642418.66 22472232.07 23006270.84 32206050.1 20027989.34 17218238.92 16739071.01 22235687.24 14704152.18 10468590.32 23347812.66 9873005.634 11425682.48 11100876.13 15217425.69 19358639.17 Arginine 124352494.3 254106865.9 182190492.5 110480019.6 108525045.8 143639393.2 81232580.15 32345857.46 144852616.3 46976175.38 201837880.8 34693502.81 23724872.67 108619094.2 113116984 31379841.61 19671581.11 25276679.61 44642760.48 156432388.3 101132975.1 30611305.8 206756868.9 21953390.35 51697268.45 114846805.4 249630420.2 57018125.05 105315275.1 114054304.6 222547255.2 134942789.2 201326850.9 52258678.37 163738054.6 Asparagine 1765768.384 3591247.374 1474728.821 340056.535 1274655.08 909174.9703 1687711.546 827428.7896 392589.5737 281794.8825 2014087.779 629665.4988 564278.2279 1845592.284 2186421.644 1081617.764 1132258.546 1455749.362 327237.1486 2724651.639 1754808.68 831353.6896 1244838.625 470687.3677 2165854.143 157789.2195 2270085.682 232803.1522 708550.9133 1202449.87 3691927.399 784529.5997 2845503.13 718656.9441 3070376.484 Aspartate 39430234.82 59158501.87 48244712.33 72621984.01 22992014.43 58706124.28 30044221.36 45193738.99 34717939.38 85673492.24 79681613.11 44040905 51663317.23 43445760.82 81790774.64 98195163.92 86485523.75 62611173.31 54377096.5 71352616.12 54977580.83 62098494.67 64771165.46 111334233.5 69415747.45 34812104.83 42791360.14 28881317.98 20779634.93 20179336.91 24122424.7 18177103.67 29844208.3 36753070.86 28464689.08 BAIBA 238591.2575 589838.1849 302197.0507 199829.839 210383.78 261386.1216 160724.102 199157.4599 247633.107 1079483.164 410324.7414 194784.493 246525.9817 231155.401 213274.0185 330208.2705 429295.997 495801.6305 864429.7498 663365.7839 649041.1538 472077.8701 589852.3382 400495.6321 305750.3629 333628.348 341169.239 323608.102 259660.558 189660.7984 136816.6165 104561.204 422752.6669 139901.7 174374.616 beta-Alanine 2285665.204 2429888.343 2752194.274 2225534.627 2096236.974 2345926.269 2159987.296 1829090.558 2216209.336 2297913.369 1322563.031 2264209.647 2205547.13 1347393.514 1684302.228 1721355.87 2178060.665 1306044.937 2036415.516 1637011.037 1898411.949 2229126.701 1184119.402 1916376.708 1489106.06 1744495.712 1394104.703 1788123.785 2304380.642 2008315.687 2149088.802 2421916.627 1667298.712 2012660.243 1348408.062 Betaine 140207476.8 140370590 79742865.4 143878758.3 168272177.1 146607600.7 121334586.9 165030867.8 308197723.8 279922502.3 140019653.1 142877783.2 246717233.7 192505085.1 153940131.6 158943634.8 160882343.4 153394646 270660404.4 100469461.8 161695452.8 119818683.5 120005667.4 162913386.3 85771517.06 142378020.6 131600357.4 122885060.9 216160270.9 107394881.1 187726635.7 165789503.2 354567646.3 120603594.8 77204629.63 C4-butyrl-carnitine 5136075.837 14652188.57 395816.0765 4221423.578 14093699.19 2085420.797 3165994.952 3479017.467 24009276.75 69348876.42 9926980.357 1210256.417 1769602.476 8469180.915 5775189.903 3486856.993 2592939.716 1691754.338 46641048.17 1661834.224 14028315.18 6259189.437 34920807.71 30842953.84 235507.9736 18386050.92 30417533.56 4457165.23 60290116.19 4007021.37 2042555.478 1547297.193 61601046.24 5288822.486 4806574.301 cAMP 39930.03853 41460.89971 53319.62221 78443.71651 95503.52665 81853.15984 123787.4805 102537.7862 55871.23087 35793.95288 65135.08918 43505.78807 44979.54679 206432.9455 82075.98389 55932.4314 79592.62258 135842.8754 29817.32585 69495.50051 97888.09062 75493.81349 102876.9648 44745.51696 680433.1228 71645.99412 129624.81 56330.5031 116278.1897 102085.3855 72859.53962 104306.9627 78468.06201 123398.5045 207484.2185 Carnitine 77173228.03 119881506.8 41088506.49 53912716.15 57987462.36 47567363.28 52349130.96 29061650.77 109418697.4 171750183 116190699.9 67112535.8 23443097.96 58801964.88 79118054.54 69155541.61 38079591.26 30685047.41 138127620.1 55605437.04 51095373.15 99305603.37 48005451.79 185694720.3 30498895.42 47221774.19 72451454.43 109326063 132483725 78117264.48 27509681.76 59326824.37 200993986.2 34167690.1 32430506.71 Carnosine 3625167.9 1842631.576 1822163.545 2969174.146 2486276.994 3655565.455 3140928.515 3581730.34 2954207.831 6193155.263 6456748.409 1981761.185 4203283.551 8931977.332 5339893.869 4402395.464 6888040.493 9187303.968 2782964.529 6606415.218 4975054.299 4609518.157 7320602.983 5542747.993 9274047.022 6531845.515 6558482.172 4348560.642 5514138.288 4828483.628 6355035.671 7582074.413 6668685.642 7678371.014 12634413.68 Choline 5292451.448 8793445.09 3443817.284 6287891.335 10699875.33 8619586.067 7277328.571 10416243.58 18786772.9 18407614.05 7756642.429 4636977.425 6754383.452 9441649.045 5750823.662 4392836.664 8171161.945 8074861.345 15364339.27 7595736.975 9328360.617 4907676.246 10278027.75 3617101.454 2964374.207 9773943.709 10520329.41 5362186.154 16800713.3 3463164.994 4136002.745 6833525.987 23357023.95 9025707.403 6132097.426 Citrulline 10321434.61 12930061.02 17040105.44 15919563.53 8441786.015 15331767.75 16426097.41 33444045.86 6980292.136 25196821.82 30178497.03 20689920.95 22216555.79 31777307.5 21265245.24 36036555.73 44589798.05 51433032.02 22397934.08 34962588.54 35999850.82 38707814.12 30998864.41 48118530.17 63269317.9 26837915.14 15464308.4 16570646.62 10737480.07 12889170.17 4683539.245 6596108.553 12953087.89 19617195.68 38378681.63 Colchicine 77391.97444 86129.98969 9788.937998 94508.21262 180045.7715 73725.3846 126683.9655 160332.4745 73319.75864 14028.2515 79413.90953 32372.213 40488.0165 42271.9045 111945.04 80160.031 89194.4795 53111.16043 33229.5595 164806.6035 44635.85 79959.97001 137949.7243 52271.201 59745.4975 45608.023 274535.6155 77561.80971 203479.8356 44722.95354 65469.40981 28273.3805 7916.3145 142712.8033 92993.377 Cotinine 53455.5145 48719.4055 47368.8425 29154.223 44301.299 34282.988 47051.9995 37758.3735 23489.181 61318.494 42291.402 39670.6565 36213.3805 54422.437 49764.8595 39015.0295 41780.92 38314.1405 73705.061 59253.7525 46146.168 33472.1475 41652.21912 57405.897 48793.767 45723.2365 59401.00649 38619.175 39871.1935 40103.927 7356.961 29834.964 7136.3395 23121.775 37208.6455 Creatine 26997744.2 33707069.3 42065253.11 50088584.34 41061791.74 41786246.22 43383294.93 40104427.42 50650664.96 35138778.96 33317877.39 36175037.17 39162335.58 40342996.79 36370338.42 39964299.67 40470547.68 33532416.56 34459215.58 32002558.26 39789855.47 32629363.81 33505125.91 34082256.17 34943440.19 46116520.39 35122817.25 39694101.66 41561781.68 41144478.47 41328921.93 48378243.88 41976351.17 39572283.39 27874149.54 Cystamine 286282.8297 336681.372 363029.5964 303564.7907 424973.866 235920.067 303773.8095 333797.53 519212.472 887467.887 515367.482 267329.4626 366166.0239 431372.7382 436745.27 407186.126 339927.5961 276575.4253 998340.3702 521640.9508 471089.4079 422133.7645 509170.6525 911582.968 702582.1555 630185.457 2431144.926 1534083.769 1864953.145 1581852.734 317391.9382 267400.805 817098.0814 261016.5345 433889.6037 Cysteamine 117859.561 193221.3035 59192.0065 143856.292 86879.7405 145766.9745 107794.4505 137884.6565 393262.3995 97670.5315 3544227.983 2306141.591 90229.4135 172286.9555 96937.3005 69400.63 106210.1775 56821.657 163357.663 106867.6375 70550.986 69048.284 102959.793 95766.1155 85317.43 109295.574 1940172.409 176971.763 2726695.3 116495.468 472804.956 219500.292 197099.4371 342882.484 189743.9525 Cysteine 21629.941 26361.7465 14207.524 12879.4755 13664.3395 10193.065 21462.84814 25214.691 116844.849 148957.938 45763.204 6830.2745 21997.408 23524.3395 18674.7715 27798.9805 14984.806 39548.593 129412.006 22303.302 65542.9895 69850.763 47469.07 9483.6025 12328.3265 15561.87 38277.5365 8032.097 115016.5731 5595.0795 21230.896 10502.908 397159.5545 11949.0975 32039.857 Cytidine 14979630.97 55482502.05 43920574.01 86984244.57 85186472.22 120877187.6 76591686.97 147141268.8 85331489.6 89780728.71 111819811.7 40500246.13 61644882.18 146796687.6 85091124.25 77072523.22 109070401.8 190505910.6 61754369.57 76623745.4 77694897.22 59315113.23 86612900.09 64250589.76 97525115.25 68252990.22 68915099.01 125667989.4 71189786.19 73971815.71 46645286.98 73269006.98 110491148 73115018.56 139817091.2 Cytosine 11233996.98 38864122.75 27415026.08 55878468.24 53858720.67 76201206.17 47263922.36 109397419.7 53540529.13 60590697.63 70668581.9 29508964.15 39814287.07 94718776.16 57144856.01 58739538.66 83154078.73 156037300.6 43442381.24 52396331.46 58330845.1 39862826.71 56531863.85 44325859.2 63834175.36 44447039.73 43704147.91 78532458.94 43970383.8 45336770.35 28641511.37 45119655.74 64253674.14 48748244.32 87491268.24 DMGV 135375.6915 120475.5841 165904.318 103573.759 257865.552 210223.5365 349053.296 133749.003 227042.0615 194689.434 142446.1291 189894.754 227481.5995 212276.5908 179479.679 141806.64 120729.4076 239994.474 177124.922 463802.0935 275066.8902 236082.6275 292461.3194 259435.284 276848.379 211467.9738 267358.612 98232.8035 169620.9424 146442.7071 172348.0943 242441.272 430902.5275 140391.8333 252215.3239 GABA.1 974.779 1171.037 N/A 4736.428 N/A 1876.4745 924.2745 549.7235 753.322 1300.129 781.836 762.975 918.873 386.7065 389.88 1720.2155 2131.67 N/A 2189.9 1335.8985 1295.891 2847.525 586.8525 1509.3915 2092.0555 1701.7915 1754.4425 1789.78 534.683 1901.564 1272.4045 1750.4465 1310.0025 914.583 2374.699 GABA.2 170292.419 383188.093 257138.3895 151429.299 184209.2787 176206.05 111957.003 147682.1255 198340.7675 697918.5027 310052.0891 177031.525 189742.9591 164087.9785 171740.0273 241544.1845 325438.1459 347170.9933 628912.8411 491168.7922 457622.8625 361816.6935 382346.5004 331851.2635 244583.2265 286063.0616 250367.113 210834.134 162638.005 145531.8426 120970.655 106697.7135 312053.9777 117135.988 138951.7076 GlucosePos2 9157.444 107274.4815 100288.8445 111696.107 41871.45233 82404.6285 54042.04758 65644.971 73460.2225 51076.03443 15482.6365 65321.411 N/A 76096.635 131128.5815 101506.7895 353205.1241 88508.4875 N/A 51570.1495 48316.635 68231.3785 54810.46108 N/A 100657.075 81365.0655 N/A 87868.1655 99547.73 N/A 68353.571 65959.161 19566.1625 12300.67287 70878.824 Glutamate 177343222.5 193110543 298608225.8 165010593.5 94587880.46 117276013.1 86362351.14 136978273.9 114827288 229808097.7 301685955.1 156419366.2 157041837.8 147494916.2 189286123.9 270223067.8 210858322.2 247747778.6 202213952.1 211301075.5 169768412.1 240262180.5 186139558.2 342031157.4 261958599.6 145016823.3 168472502.8 126534819.1 92327966.87 205425515.2 120950512.9 112067827.7 99103211.62 103035772.8 129764197.6 Glutamine_spec 3203744.737 4130370.983 4559205.421 2020077.617 1885445.773 1610112.285 1569259.541 1154565.376 1753530.219 3022535.088 3265444.862 3517933.403 1807516.396 1688422.686 3093570.855 3441278.352 2595797.773 1308213.754 4028507.237 4425258.171 2117356.699 4491533.155 2546526.446 5090307.857 4500263.026 1978888.381 2403153.72 3019112.633 1923950.884 3572878.369 2216917.897 2233643.889 1252433.876 2431868.634 1825491.868 Glycerol 5876.627 1808.3465 6492.466 1791.647 4928.7595 1193.2315 1547.807 7073.326 2474.273 1853.6545 2562.758 2405.248 1457.651 2604.8315 1104.863 4904.869 1456.223 6629.6525 1591.9355 2256.239 10249.33 1325.238 3336.174 2161.868 906.6 1103.0795 1057.9155 4550.293 3622.008 4063.4515 1833.3315 3709.503 10458.734 5519.311 5920.928 Glycine 231570.8555 351754.632 321143.607 204529.857 284292.332 209518.471 220028.3855 361079.2135 559649.849 645404.908 490016.466 206885.0825 256391.92 439818.676 268771.738 312848.7285 400022.2265 946992.931 519748.0205 434600.63 368988.3955 313304.2205 530342.3405 416519.116 421369.332 374030.658 427436.8865 188097.9375 408931.0635 171573.0925 234822.423 143835.3203 967375.983 189296.1425 252596.723 Glyoxylic acid 29207.0635 17256.985 21708.9275 65616.7135 181465.9605 13076.539 11879.9865 147763.3485 25132.4128 21517.607 86755.863 16245.598 38875.6355 24300.6525 8061.6405 15090.0245 37081.747 58526.058 15860.2335 28767.6595 13651.9105 25167.145 21690.376 65883.794 28962.236 31307.531 45572.9645 50620.131 14290.7905 57214.3095 61963.557 33099.839 18252.8565 30391.647 41106.6695 Histamine 799193.4773 983915.3876 1831351.514 1249135.227 682145.4031 733759.5143 860143.5996 1156693.978 1390722.074 1815663.332 1827163.905 912906.4578 1584925.154 1111262.989 1259157.768 809567.122 816853.4336 1070543.741 770625.6009 589831.5133 597419.693 1272081.573 1405669.117 982859.1919 1093750.413 564156.9533 922010.5191 832861.0323 1051916.67 1568078.603 1518130.314 661260.618 2656407.96 943482.7836 934739.7989 Histidine 27938600.56 68090826.27 28316170.68 27709145.43 40240462.63 50422901.18 37889774.86 82910870.13 40841314.65 93038136 110966685.9 27786837.08 55392621.69 51081671.86 38211175.23 58936582.06 76606354.45 150609732.2 42972114.48 64680482.57 60148308.86 43286673.17 71311079.94 34630089.72 40940029.9 29093923.99 58451609.74 18229929.69 33451093.58 13820725.19 41333692.19 16956653.36 65047806.8 22335072.78 26903411.19 HomocysteineN 1371383.39 1176331.696 1710157.903 3673063.649 1160907.241 1093795.211 1184901.091 2491318.981 1098197.311 2115278.351 2308684.631 1108907.126 1376260.595 1296921.937 1319809.524 1931456.319 1245815.743 1355330.048 2029815.335 1207360.528 1070606.036 1188207 1168987.843 2628470.761 1381849.278 1428992.671 1685070.943 5112471.749 1642088.554 1598697.372 4883043.371 1864393.71 1833522.735 1818292.869 4469436.94 Isoleucine 2600356.347 2031755.635 5551522.382 4323807.707 3133185.512 2984720.454 4549257.868 3388161.436 4565799.348 6491412.661 13166643.28 3267179.201 4760011.577 8145588.736 6003032.514 4685768.228 7884918.264 16768208.22 4061820.346 15989688.65 13665953.37 8911210.417 22468459.04 7602316.16 15701429.16 10272059.32 13110719.6 1917781.576 5041502.658 3419673.346 12037154.72 4014725.756 13949096.06 6541638.293 9547036.408 Isoleucine_Leucine 43533390.82 33112747.8 86948560.04 74446816.26 57079338.24 51378864.4 80903325.02 58700024 78425046.05 118830826.9 224614420.6 60179255.33 82426797.91 135105277 107541944.3 79536378.36 142365009.6 259826128 68752445.64 238712267.4 212233515.9 144724277.4 371078978 121027743.7 255997235.1 171657677 231336803.6 31632058.37 89593756.21 55454884.45 180585286.8 67527037.58 237722137.2 101597410.8 177080647.6 Isovalerylcarnitine.1 1059238.334 3199107.22 130311.0875 530438.367 2547827.706 502515.0285 832707.677 740424.718 11397506.97 24994307.69 1880886.227 302298.2515 372473.0215 1578242.865 795141.911 576772.067 572167.8605 337694.655 15404667.76 426403.1635 1713256.579 811362.9294 3687474.98 4603849.932 223570.0521 4695599.684 4231939.169 1175095.796 20072998.95 678848.3765 484474.2675 455703.434 33608842.23 1322789.448 1289251.06 Isovalerylcarnitine.2 473599.819 1215159.81 62018.36168 190579.716 1028351.063 286337.5984 308639.6959 322749.8055 4295831.731 9218906.394 753710.944 144072.7919 169365.7802 690567.8832 325331.886 207157.1394 206960.9845 144672.1703 5895775.119 129339.2831 709920.15 349131.3865 1293258.028 1688427.937 100416.3346 1900561.882 1759560.965 427744.0415 7578845.359 336151.5492 168732.239 238314.881 12943753.51 503139.935 457388.0806 KIV 6718.228 12052.3005 8239.3135 5980.3635 3843.03 10663.4135 12845.8835 17247.5345 8553.9065 3537.057 12522.1035 17125.251 13860.86566 10422.071 6836.3345 6701.8605 11874.474 15143.34598 5925.295 12267.8125 5081.463 24329.9145 20538.80132 6592.2305 5335.886 8074.978 6761.789 12742.8855 13250.317 9933.555 5660.027 14770.3335 4822.017657 2730.049 16502.049 Kynurenic acid 6607665.632 15925641.29 12546465.55 10537909.56 14621085.34 9287782.392 20361557.61 15821496.42 11195216.3 3134883.123 1769331.609 5249493.055 11230152.36 11079749.03 5587717.643 8274017.295 5198041.097 7736636.203 6651633.694 5654702.603 8267329.555 5281294.404 8147609.268 22710950.86 17998894.26 12249996.09 31605442.63 16865987.78 11648976.84 16960051.92 9425824.683 12186425.19 12742843.94 13453516.43 14272124.91 Leucine 12723081.6 13835137.97 4817176.437 7466015.406 12306023.76 2990013.676 2890428.36 4072384.608 30225337.1 41887916.8 15826653.44 4294292.934 4969827.51 13617596.93 8786460.516 8979125.522 6400956.11 3714589.138 37290901.34 6585536.446 19699582.27 9518569.082 21768609.36 19823295.71 5483024.934 16767886.95 20299826.98 8874642.142 36923744 8017108.587 6245842.568 13097881.76 51588656.31 7784230.099 8982345.365 L-Homoserine 4618155.46 4753711.494 6595825.616 5486497.668 4318095.523 3938122.335 5178932.759 5183488.115 6262818.079 8266960.628 12949436.93 4323684.662 5411807.631 8615157.098 6074574.483 6352479.861 7551307.608 12953836 6123355.961 12070322.07 10834029.23 9021290.834 14650331.02 7295092.297 10316309.87 8898739.01 10966789.35 2160096.79 5542650.03 4059584.174 8200495.184 2861111.553 14123876.44 4562458.726 7996123.312 L-NMMA 3331580.207 3384268.065 3024786.161 1657342.338 2226341.876 2673088.069 1500562.403 1244445.813 2519149.866 5157887.252 1813777.969 2737312.524 1612704.841 1892637.824 1826680.96 2500209.89 3465047.083 2227777.087 4281638.075 2339029.526 2497985.214 2173044.103 2329334.827 3487698.463 1616770.759 2869879.243 2264335.428 1780334.702 2019061.332 1821863.144 968544.8383 1139040.714 3019543.579 1570955.03 749009.0308 Lysine 5885239.61 14513501.36 19522483.19 12926997.36 14841219.14 13501422.37 16100876.67 15368339.26 18456321.72 24101788.48 30866157.89 10907905.41 13924432.08 28602055.26 18693317.11 18527974.38 21280508.29 32782426.76 15141237.84 34360960.25 30189461.79 20827732.91 42112636.31 15876802.05 21266471.19 20417498.41 31470942.52 5736778.231 22494081.01 11839619.24 21892549.21 8256966.404 59786655.44 8837597.053 24929438.14 MES 3264.024 2868.0635 2992.1915 2885.073 4138.1975 4475.953 3709.335 4389.1715 5154.8005 3219.8885 4043.66 2679.828 3408.666 4110.949 3629.529 3679.3245 6534.219 3167.1445 5609.818 5247.7425 5235.2395 3483.0535 23504.3615 5434.509 3728.9785 6927.6955 3307.3235 7517.0335 2678.1375 8233.032 4156.261 3511.384 2526.9685 2652.207 4838.0205 Methionine 3000494.738 2487471.713 5872772.713 4156536.193 2839564.74 3538664.552 3971709.521 4089028.811 3999600.955 6961377.959 11278622.2 4234310.117 5389020.737 6624525.86 7040050.811 6317327.361 9381315.748 11751664.67 4555813.029 11298757.99 10910802.98 8224043.57 16426216.01 8990845.776 15968028.68 10055931.8 11442387.15 2721526.343 5187763.241 3977858.423 8786057.478 3934606.321 12023414.46 5454695.422 9888205.678 MetSul 6326444.356 7557222.242 12022825.26 7294671.292 6316127.462 6906071.922 8999498.722 9467753.348 7605643.313 14402067.39 21941744.48 6383042.629 7700530.584 12519241.18 9003228.073 8551558.025 12515272.28 22464825.65 8301557.841 20875456.94 17200951.54 12759341.04 29962749.93 13051487.93 22557397.73 16114892.65 18040369.3 3137259.34 7286918.798 5581074.558 15143531.05 6252858.691 19768246.06 8931448.248 16311394.32 N-acetylglutamine 9988315.017 18240494.96 14453620.78 10568457.12 11789507.17 12538865.1 11993431.26 7347974.88 11799378.68 20314506.59 14514644.52 8765362.854 9257730.905 16638217.7 11509832.45 13898360.06 14462060.17 12224029.51 17826141.92 15949910.93 16401880.87 13496234.3 22007475.63 15943377.57 17536936.02 14311607.15 17653080.44 2423451.016 9304029.374 8362306.987 6689779.154 7747209.077 16428306.79 10608036.18 4761630.807 Ornithine 4985139.23 11362085.93 6391476.158 6001894.13 7174312.853 7343637.141 4473468.362 12776991.61 7125195.012 12094609.85 10793791.29 7751159.228 8360671.575 15768238.33 7555318.478 12085034.87 20581894.56 31356816.71 13159701.97 10567966.8 11551289.31 9427397.024 16604393.91 11320428.27 6060720.411 7608397.553 7158944.933 4348254.919 4814713.948 2198849.258 2575629.337 2457623.614 9194753.176 3083482.656 9849451.583 Phe-d8 29209655.92 30191114.93 30915819.59 29139986.95 26238338.41 31271742.2 28062586.56 31682477.87 28454227.16 28609130.5 32091402.64 28108285.29 27799198.18 28256389.06 27785265.18 29647508.39 32959461.74 30206968.12 32735841.62 36544281.74 30335834.13 29877425.04 29718578.59 32678965.41 30034100.06 29778055.17 31761404.49 28973757.69 29035943.05 29443601.38 29143576.6 29289553.64 32034700.84 29733225.27 30165120.65 Phenylalanine 29034794.2 22515225.43 62298496.34 51201521.42 35133129.14 36026114.17 42698301.31 41629245.26 59274395.08 89623670.9 162905511.1 47865646.73 81710666.35 89299177.48 90811091.04 65320565.52 120268464.1 161523039.5 57391373.93 164306650.4 167947453.8 106919288 262439640.5 86491456.32 196948932.3 133734702.2 156672706.8 25325651.08 59491908.86 39787250.9 120413050.9 48607827.92 175413206.7 68466492.88 129818553.2 Phosphocholine 5551191.185 5367229.936 5172386.803 5803098.426 5418091.591 5394479.952 4823273.871 5927863.006 6027095.191 5048805.6 5787621.155 5531923.528 5731593.964 5600046.523 5055636.959 6239098.228 5443211.384 5512856.971 5622898.829 5215775.48 5220479.259 5797004.377 5758710.357 5641502.603 5577270.38 5480851.552 6240807.266 6063627.919 4742235.68 6407727.707 5488512.514 5597139.943 4728484.705 5266313.183 4525996.71 Phosphoethanolamine 120299.7058 275186.7065 186959.637 107288.23 116068.002 129522.808 231705.6685 344600.168 151405.2002 197291.811 208045.068 86944.179 108606.1755 275424.137 192723.834 175082.1886 136621.21 87111.157 226771.051 142610.748 111368.7598 126900.187 153754.6925 207228.8465 204154.5635 119246.565 175955.7495 112620.408 130232.042 124974.0115 144139.572 161329.741 219301.7125 148756.808 125882.4135 Proline 115088048.1 218221678.5 186088718.4 119016943.7 141463451 127551340.1 156080615.1 193501260.9 106837116.8 220617383.4 248785915 129761769.8 156595141.3 181396796.1 187675068.4 162496587.9 214483642.3 252208829.1 189411767.4 223151894 199746444.6 169434620.8 196187883.4 192666534.8 185774434.9 177884031.8 176176942.8 125536729.9 110588967.2 89111406.87 162587995.6 111469760.5 188225388.3 112105091.6 121747226.8 Propyl-l-carnitinne 29316.63 58484.03757 53498.755 115326.189 47765.5695 129305.5215 59869.13281 415611.6415 51142.678 41581.0455 91698.6675 77910.215 35015.54808 69256.677 49266.018 74883.417 70388.168 94877.231 64589.454 76049.8275 46620.984 54836.4785 111028.3415 80423.333 54577.0355 120054.697 61210.8125 97673.8295 47653.749 97429.7795 109412.6155 59841.5235 91369.7605 47001.002 106466.5007 Purine 182442.8244 299394.0375 347187.25 288680.5396 358010.4329 377653.851 450674.482 321951.3618 212999.2268 387378.8169 475233.6625 332989.5635 445623.0295 581421.1005 484295.753 207773.5182 499046.3655 433641.4843 202789.7751 625111.859 598998.4125 341360.0252 568413.107 269457.1426 564824.0915 328921.234 369436.2475 133081.9568 176937.8389 184375.9091 517283.7525 449514.9965 330483.8244 429638.742 340066.3803 Pyridoxine 420551.4665 657947.0325 474619.5885 408913.7194 458538.5476 160995.121 168705.113 215882.7165 405096.6823 243080.7535 308194.7158 416487.3615 262355.289 648005.797 753849.391 228210.884 521567.5135 479877.507 691553.7 359278.597 483302.6634 571359.0945 447320.2275 537758.257 249942.2405 719042.657 489464.792 351098.7705 919514.001 483592.322 411914.5125 598035.0425 721179.3412 264397.172 213782.052 Riboflavin 44862394.55 30031574.88 66932528.3 26603753.15 22045451.83 34978811.5 35243093.06 24718718.93 25546916.33 96296591.29 99675304.67 54785929.99 51299687.52 62141805.56 101262452.7 109370393.8 89032280.1 62844105.76 103841668.1 119718408.2 117371843.7 107818037.5 200165160 171441867 188812231.2 121074262.5 92606230.92 33082817.75 28438997.37 44662123.44 37072941.85 27569103.27 19899039.22 44719083.71 94422270.94 Serine 628902.4807 695103.5628 933058.739 607962.1905 808713.654 612437.2043 780273.4008 652808.336 955731.6878 1228498.367 1382552.304 694761.2798 695618.5575 1229419.185 928532.4505 948840.206 829495.3523 1094123.761 1141712.232 1331371.317 1755636.229 1189144.391 1958390.242 893323.0437 1595978.736 1286233.754 1697265.858 683698.4822 1029015.125 583772.1505 1222030.969 646776.25 2515883.23 610059.3134 1246629.287 Serotonin 1985659.036 2702385.974 3110103.036 2947022.409 1677696.51 2874996.763 2604544.146 6545096.219 1391595.555 4808580.369 5355771.987 3786086.757 4231694.74 6187972.149 4018683.334 6536637.014 6947511.628 9484307.626 4129389.186 6128232.632 6839733.551 7236319.718 6038098.967 8611545.583 11600515.7 5075029.999 2960933.964 3050084.335 1988270.237 2355510.341 1190226.857 1266504.059 2437395.294 3354674.952 6890174.497 Spermine 19179980.16 30371166.87 20349159.64 7776012.172 21888396.89 25591712.52 26364648.45 6008553.803 9971207.187 57304989.44 25423285.59 17875869.84 19650941.17 19863014.72 30198639.37 23760151.9 41618695.1 27931235.67 37290801.19 35299850.93 26289138.16 23664658.41 23954499.85 24546937.31 37419769.61 19716931.62 20231266.86 6493131.247 11561881.2 7045273.453 4842448.448 5390240.359 11082341.42 10744189.35 5140909.898 Taurine 15715057.21 34708949.92 15670363.14 23771963.06 45332083.44 41568132.36 23471949.33 34501663.14 71593821.1 72981733.58 45656810.85 13980228.05 73161170.72 94319139.84 32247372.26 32143791.34 28505486.36 100893967.1 54843284.26 39015906.1 30732154.36 19396525.25 55455256.94 19471739.54 7416444.427 29489287.2 38478049.04 7130005.622 93799728.37 6655874.809 6208929.279 8437756.357 131813063.4 33621763.36 68300285.48 Thiamine 3394961.161 2190210.954 2407751.253 1320352.074 2456065.398 1714182.729 2627035.979 2745455.053 2648408.138 9494707.688 8009516.385 7128290.632 5554582.763 7223165.972 10765950.62 5273524.425 9380783.063 8902562.08 6570405.449 9576220.553 9817520.671 6113657.073 10547886.6 4483162.784 6490227.956 5910750.975 10545298.59 1263143.325 3236085.104 3037603.949 3078279.42 2200988.782 4722214.915 5622624.668 2712362.543 Threonine 4671950.425 4634718.414 6775007.401 5339323.107 4432501.565 3831535.95 5147567.806 5382442.895 6163093.782 8053806.87 13093148.42 4310884.685 5438390.404 8758562.293 6000104.729 6457949.297 7680160.095 12810781.53 5889860.393 12153904.32 10937736.93 8983846.589 15197110.57 7476969.656 10474316.59 8920634.538 10702751.13 2154367.327 5485628.97 4014297.711 8314720.16 2823589.092 13930490.73 4676389.677 8190352.21 Thymidine 630265.1781 627923.6145 566872.895 446635.5408 476795.4204 696224.9601 582746.979 589468.0401 881821.5292 865338.641 1251612.485 508990.4322 724819.257 588102.001 871311.1511 1316532.026 673016.8356 450553.976 485984.8462 656089.553 437119.9005 839539.9668 650989.0999 610951.0548 1122445.017 909261.7229 882900.7809 316407.0858 178265.411 911387.307 261211.8372 381938.3005 83126.889 211519.2365 143775.86 Thyroxine 149548.7683 230590.4425 167888.5985 74292.848 123265.4605 101694.584 136532.0625 82487.668 74254.055 217894.757 202983.2045 117028.812 68387.4195 95594.975 189059.367 131500.1165 109846.087 130884.842 177747.6005 258158.831 96870.336 195815.7625 224389.4355 277220.14 219538.182 141431.687 221277.4455 170401.1125 96932.32 204113.4645 102047.8635 69184.653 83174.884 103955.1926 155459.507 TMA.1 26941805.43 41394193.87 25290246.99 25254193.45 35220881.74 30104077.11 25391403.63 31783760.32 54589387.39 62385557.55 35196845.09 25127218.86 22296994.28 31182405.66 26432589.22 25452864.3 31202346.21 26726969.38 56731138.81 36504526.98 30895062.91 30245049.76 37765627.67 32886939.66 23340553.76 32771841.26 37261965.38 25443923.04 51579901.49 22762125.58 18369415.47 25315123.53 68225764.81 30019836.6 22095127.9 TMA.2 69235041.09 108038180.8 69995229.38 69390248.97 90081874.15 75887958.43 66577088.98 83441398.87 142704629.1 150290609.1 92637778.83 69019880.06 62908665.06 85064414.74 71644131.52 66905494.86 80759547.83 67988740.97 140666561.2 95977133.05 78296873.52 81205604.56 96354122.85 84637957.58 63268865.68 88045383.22 91390780.84 69898832.51 135675127.4 59892129.35 47930017.1 64038214.78 175690166 81757584.51 61415767.19 TMAOd9 204996393.2 198489896.3 189795962.8 200901132 188013791.4 212873914.2 184747364 195047649.3 176828182.3 183805360.6 178697293.9 206866388.6 216298477.5 169022663.1 190684620.5 194142911.5 214642365.4 187517130.3 196060853.2 208280688.9 171894124.7 191132399.3 154445113.6 197898543.9 174913200.6 181188626.1 163039003.7 208048813.2 167108436.3 190944441.4 189165791.2 210860682.8 156268761.3 182338905.7 135559817.6 TMNO 1934912.669 3154402.07 2928003.117 2144732.058 5293076.197 2112365.114 1479656.122 2188680.207 6893939.873 4120453.915 1219062.459 1822917.806 1650115.476 4752830.009 1772768.759 1792710.913 1592373.406 1789054.439 6868019.6 1473660.911 3011208.023 1997086.158 2766473.796 1586156.15 1070518.254 1361476.097 1668091.774 2074514.374 28430717.93 1552770.298 1608418.44 1795579.756 14339984.21 2605727.234 3193348.751 trans-HYP 18216930.15 23894966.75 29140055.48 35263915.13 29441464.82 28694351.2 29418599.48 30661709.41 34197779.56 24686888.49 23104062.41 24207507.34 27886162.75 29719465.64 24593008.47 27229783.71 28099874.11 24073402.87 25074252.72 22758601.38 28216087.18 23261014.95 22322952.56 23427724.36 25797921.05 31213439.92 23891760.76 26739464.35 29324288.02 27736385.59 27598298.9 33405515.67 29747230.56 27029737.95 18880111.45 Triidothyronine 109247.8105 133661.7226 96930.16083 58067.45842 143838.761 151413.5618 97074.59333 16257.48035 N/A 239177.9065 96975.2215 71969.93373 72423.832 54968.11629 130898.3292 254878.878 94522.23703 80860.45855 376436.411 138440.217 157722.806 226683.8935 207584.6684 207259.7844 363004.684 175952.0535 142823.5948 92029.91913 68641.59798 142176.9768 64845.92805 108188.9882 104423.6665 66411.7475 78908.26963 Tryptophan 15394255.94 24877186.51 25716444.79 22500036.2 35252459.75 25389696.43 35367899.41 45650844.17 22330234.34 27003916.36 62635375.24 19188771.76 29293543.02 42442429.09 36529232.93 21835670.75 35706482.98 148710992.8 22671596.43 62796427.25 56282753.92 31039806.41 90063537.91 32698093.51 46293143.58 39167100.82 44525449.86 8233331.194 16185821.89 16070057.9 38122868.8 14295076.82 67650074.43 21231910.5 33738612.76 Tyrosine 6265910.893 7596985.66 12229189.25 7121642.056 6537023.862 6964497.284 8532479.716 9234265.379 7598095.405 14235195.23 21841014.22 6148042.849 7578878.656 12190345.45 9047052.662 8299990.91 12395885.82 22101399.41 8272840.972 21155655.89 17378395.6 12588181.65 29211072.44 13111195.96 23270938.96 15469345.88 18220813.5 3228985.357 7198316.139 5503738.385 14498814.15 6306602.954 19538266.75 8739255.753 15794180.84 Uridine 14055040.85 14181547.19 23498998.04 18081573.18 9157804.383 10934652.35 12585215.96 15169737.13 20693112.05 23193086.15 28321680.25 12764805.1 24903293.85 18003689.36 17192527.08 11269336.3 13847961.18 14779807.78 12755569.84 9507164.813 8034281.421 18601668.13 21461802.57 16598399.22 18435579.3 9457889.444 16274026.66 11874794.28 13342454.24 22484736.94 19369910.73 9341556.466 36174131.48 14412778.66 14198591.19 Valine 18075411.99 16824841.25 26724295.51 28003114.72 23453787.16 19854647.73 31710165.61 19350437.18 25142004.64 43862998.6 59576442.88 20025940.67 27140556.56 49558602.75 31116190.38 22391745.49 39549723.72 92117211.78 25548506.22 69854043.48 55086747.85 40176993.83 100142711.1 37513791.33 64094182.8 49211123.47 60144174.55 10952223.62 25068071.03 17666963.45 54940719.53 19685121.92 65122708.24 27387417.11 40746264.32 Valine-d8 11020105.27 8287180.751 9052424.395 10375863.74 9221996.774 10496150.33 9212110.345 9589908.76 9956651.729 10062769.64 7618019.227 10084257.36 10543225.52 8518205.382 9000551.332 8885880.955 10669788.54 8236498.769 10645849.37 9646154.524 9247769.5 9353206.565 7881378.87 9309192.728 8438672.696 9488115.163 8941289.381 11243847.15 9412177.439 9592028.286 9258686.549 10176512.02 9547057.049 9540744.818 8603591.3 α-ketoisocaproic acid.1 207051.534 211555.919 427961.5565 107540.7136 376788.4605 269753.36 341025.772 78740.446 144253.3946 94989.3875 117494.2815 105000.338 46796.706 28427.036 115250.098 76260.3435 74256.1035 150694.6335 152686.6155 117879.176 166694.5305 117634.8925 99309.735 221233.9675 218676.086 332867.1825 86042.1315 198776.5605 109920.4815 28380.9195 206292.6135 157003.738 111238.8665 63573.0245 48863.80399 α-ketoisocaproic acid.2 3076.8965 2254.742 6085.926 4044.8135 5971.658 7970.396 7317.67 2779.7315 15491.0055 5492.086 5216.4705 1968.764 3670.0025 3272.691 5597.577 9029.425 4486.9395 3950.0185 8023.46 3455.6605 5054.8215 4145.7995 6213.404 11213.1445 4377.6965 6870.702 5565.32 2990.999 3515.0145 6614.853 3741.9385 6185.9655 4923.248 6495.8255 4755.066 α-keto-β-methylvaleric acid.1 103718.251 72708.314 45227.175 289498.9085 210071.6875 138680.7075 210557.451 368467.2545 193698.415 74662.7945 39898.9475 87331.03787 103493.954 128530.2185 77367.5155 96786.613 78847.9375 115081.021 179777.3825 87805.0915 148620.362 88705.304 37274.199 79246.8785 79853.9975 115352.4475 50103.723 161828.4465 62567.0115 35207.1705 151573.16 153877.732 61606.9875 96491.723 30912.599 α-keto-β-methylvaleric acid.2 473328.7475 880000.3185 666587.165 779484.489 582963.2675 812444.7525 482941.3795 1301311.173 642562.7657 1001471.581 1208582.972 736625.422 735703.7437 1131744.686 879408.007 906292.72 1165829.466 1394828.015 803412.337 1114839.716 1126004.452 1168323.391 1407410.651 1032665.925 1201244.028 1095185.052 1113743.686 647311.141 656046.5635 407647.083 669617.3695 623119.0895 857520.955 673242.889 1243299.191 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name RefMet Name/Standardized name PubChem identifiers KEGG 1-methylhistamine 1-Methylhistamine - C05127 2-Arachidonyl glycerol MG 20:4 - - 2-Methylbutyrylcarnitine.1 CAR 4:0;2Me - - 2-Methylbutyrylcarnitine.2 CAR 4:0;2Me - - 2-Methylbutyrylcarnitine.3 CAR 4:0;2Me - - 2'-deoxyadenosine Deoxyadenosine - C00559 2'-deoxycytidine Deoxycytidine - C00881 3-deaazadenosine - - - 3-IPA 3-Indolepropionic acid 3744 - 3HK Hydroxykynurenine 11811 - 5-Aminolevulinic Acid 5-Amino-levulinic acid - C00430 5-HIAA 5-Hydroxyindoleacetic acid - C05635 AAD Aminoadipic acid - C00956 Acetoacetic Acid Acetoacetic acid - C00164 Acetylcarnitine CAR 2:0 - C02571 Acetylcholine Acetylcholine - C01996 Adenosine Adenosine - C00212 ADMA Dimethylarginine - C03626 Alanine Alanine - C00041 Anandamide Arachidonoyl-EA - C11695 Anserine Anserine - C01262 Arachidonic acid Arachidonic acid 444899 - Arginine Arginine - C00062 Asparagine Asparagine - C00152 Aspartate Aspartic acid - C00049 BAIBA beta-Aminoisobutyric acid 439434 - beta-Alanine beta-Alanine - C00099 Betaine Betaine - C00719 C4-butyrl-carnitine CAR 4:0 - C02862 cAMP 3',5' cyclic AMP - C00575 Carnitine Carnitine - C00318 Carnosine Carnosine - C00386 Choline Choline - C00114 Citrulline Citrulline - C00327 Colchicine - - C07592 Cotinine Cotinine - - Creatine Creatine - C00300 Cystamine Cystamine - - Cysteamine Cysteamine - C01678 Cysteine Cysteine 5862 - Cytidine Cytidine - C00475 Cytosine Cytosine - C00380 DMGV Dimethylguanidino valeric acid - - GABA.1 gamma-Aminobutyric acid - C00334 GABA.2 gamma-Aminobutyric acid - C00334 GlucosePos2 Glucose - C00031 Glutamate Glutamic acid - C00025 Glutamine_spec Glutamine - C00064 Glycerol Glycerol 753 - Glycine Glycine - C00037 Glyoxylic acid Glyoxylic acid 760 - Histamine Histamine - C00388 Histidine Histidine - C00135 HomocysteineN Homocysteine - C00155 Isoleucine Isoleucine - C00407 Isoleucine_Leucine Leucine/Isoleucine - - Isovalerylcarnitine.1 CAR 4:0;3Me - - Isovalerylcarnitine.2 CAR 4:0;3Me - - KIV 3-methyl-2-oxo-butanoic acid 49 - Kynurenic acid Kynurenic acid - C01717 L-Homoserine Homoserine 12647 - L-NMMA Targinine - C03884 Leucine Leucine - C00123 Lysine Lysine - C00047 MES methanesulfonylmethane 6213 - Methionine Methionine - C00073 MetSul Methionine sulfone - - N-acetylglutamine N-Acetylglutamine - - Ornithine Ornithine - C00077 Phe-d8 - - - Phenylalanine Phenylalanine - C00079 Phosphocholine Choline phosphate - C00588 Phosphoethanolamine Phosphoethanolamine - C00346 Proline Proline - C00148 Propyl-l-carnitinne CAR 3:0 188824 - Purine Purine - C15587 Pyridoxine Pyridoxine - C00314 Riboflavin Riboflavin - C00255 Serine Serine - C00065 Serotonin Serotonin - C00780 Spermine Spermine - C00750 Taurine Taurine - C00245 Thiamine Thiamine - C00378 Threonine Threonine - C00188 Thymidine Thymidine 5789 Thyroxine Thyroxine - C01829 TMA.1 Trimethylamine 1146 - TMA.2 Trimethylamine 1146 - TMAOd9 - - - TMNO Trimethylamine N-oxide - C01104 trans-HYP trans-hydroxyproline - C01157 Triidothyronine Liothyronine - C02465 Tryptophan Tryptophan - C00078 Tyrosine Tyrosine - C00082 Uridine Uridine - C00299 Valine Valine - C00183 Valine-d8 Valine - C00183 α-keto-β-methylvaleric acid.1 (S)-3-Methyl-2-oxopentanoic acid 439286 - α-keto-β-methylvaleric acid.2 (S)-3-Methyl-2-oxopentanoic acid 439286 - α-ketoisocaproic acid.1 Ketoleucine 70 - α-ketoisocaproic acid.2 Ketoleucine 70 - METABOLITES_END #END