#METABOLOMICS WORKBENCH BDrotleff_20250207_014941 DATATRACK_ID:5600 STUDY_ID:ST003735 ANALYSIS_ID:AN006128 PROJECT_ID:PR002321 VERSION 1 CREATED_ON February 18, 2025, 10:40 am #PROJECT PR:PROJECT_TITLE Evaluation of in vitro pharmacodynamic drug interactions of PR:PROJECT_TITLE ceftazidime-avibactam with tigecycline in ESBL- (extended spectrum PR:PROJECT_TITLE beta-lactamase) and carbapenemase producing Escherichia coli PR:PROJECT_SUMMARY Background: Combination therapy offers a promising option to enhance efficacy PR:PROJECT_SUMMARY and prevent resistance. A comprehensive and quantitative assessment of the PR:PROJECT_SUMMARY last-resort combination of ceftazidime/avibactam and tigecycline is not PR:PROJECT_SUMMARY available. Objective: This study systematically investigated the pharmacodynamic PR:PROJECT_SUMMARY interaction between ceftazidime/avibactam and tigecycline in clinical and PR:PROJECT_SUMMARY isogenic Escherichia coli strains harbouring genes that encode various PR:PROJECT_SUMMARY carbapenemases or ESBLs (extended spectrum beta-lactamases). Methods: An PR:PROJECT_SUMMARY adaptive in vitro 'dynamic' checkerboard design and pharmacometric modelling PR:PROJECT_SUMMARY were employed for the evaluation of pharmacodynamic interactions in fifteen PR:PROJECT_SUMMARY bacterial isolates. Additionally, time-kill assays and metabolomic analyses were PR:PROJECT_SUMMARY used to provide mechanistic insights. Metabolomic analysis: Mechanistical PR:PROJECT_SUMMARY investigation of the PD interaction between ceftazidime/avibactam-tigecycline PR:PROJECT_SUMMARY was studied in a selected clinical isolate of E. coli (strain JUM_JEA) using PR:PROJECT_SUMMARY metabolomic analyses in mono- and combination treatment scenarios. Time-kill PR:PROJECT_SUMMARY assays were conducted for ceftazidime/avibactam and tigecycline concentrations PR:PROJECT_SUMMARY of 4 x MIC (minimum inhibitory concentration) as well as combinations of both PR:PROJECT_SUMMARY antibiotics at 4 x MIC CZA – 4 x MIC TGC and growth controls as four PR:PROJECT_SUMMARY replicates over 4 h with samples at 0, 2 and 4 h. Results: Antagonistic drug PR:PROJECT_SUMMARY interactions between ceftazidime/avibactam and tigecycline were identified in PR:PROJECT_SUMMARY the majority of tested strains. Time-kill assays confirmed antagonistic PR:PROJECT_SUMMARY interactions, with tigecycline limiting ceftazidime/avibactam total killing. PR:PROJECT_SUMMARY Metabolomic analyses of mono and combined drug exposure to bacteria revealed PR:PROJECT_SUMMARY matching metabolomes in tigecycline alone and the combination with PR:PROJECT_SUMMARY ceftazidime/avibactam, corroborating the identified antagonism between these PR:PROJECT_SUMMARY drugs. PR:INSTITUTE European Molecular Biology Laboratory PR:DEPARTMENT EMBL Heidelberg PR:LAST_NAME Drotleff PR:FIRST_NAME Bernhard PR:ADDRESS Meyerhofstr. 1, Heidelberg, BW, 69117, Germany PR:EMAIL bernhard.drotleff@embl.de PR:PHONE none PR:PUBLICATIONS https://doi.org/10.1016/j.ijantimicag.2025.107457 PR:CONTRIBUTORS Aneeq Farooq, Bernhard Drotleff, Niklas Kroemer, Mei-Ling Han, Jian Li, Jean PR:CONTRIBUTORS Winoc Decousser, David Schrey, Julien Buyck, Nicolas Grégoire, Patrice PR:CONTRIBUTORS Nordmann, Sebastian G. Wicha #STUDY ST:STUDY_TITLE Evaluation of in vitro pharmacodynamic drug interactions of ST:STUDY_TITLE ceftazidime-avibactam with tigecycline in ESBL- (extended spectrum ST:STUDY_TITLE beta-lactamase) and carbapenemase producing Escherichia coli ST:STUDY_SUMMARY Background: Combination therapy offers a promising option to enhance efficacy ST:STUDY_SUMMARY and prevent resistance. A comprehensive and quantitative assessment of the ST:STUDY_SUMMARY last-resort combination of ceftazidime/avibactam and tigecycline is not ST:STUDY_SUMMARY available. Objective: This study systematically investigated the pharmacodynamic ST:STUDY_SUMMARY interaction between ceftazidime/avibactam and tigecycline in clinical and ST:STUDY_SUMMARY isogenic Escherichia coli strains harbouring genes that encode various ST:STUDY_SUMMARY carbapenemases or ESBLs (extended spectrum beta-lactamases). Methods: An ST:STUDY_SUMMARY adaptive in vitro 'dynamic' checkerboard design and pharmacometric modelling ST:STUDY_SUMMARY were employed for the evaluation of pharmacodynamic interactions in fifteen ST:STUDY_SUMMARY bacterial isolates. Additionally, time-kill assays and metabolomic analyses were ST:STUDY_SUMMARY used to provide mechanistic insights. Metabolomic analysis: Mechanistical ST:STUDY_SUMMARY investigation of the PD interaction between ceftazidime/avibactam-tigecycline ST:STUDY_SUMMARY was studied in a selected clinical isolate of E. coli (strain JUM_JEA) using ST:STUDY_SUMMARY metabolomic analyses in mono- and combination treatment scenarios. Time-kill ST:STUDY_SUMMARY assays were conducted for ceftazidime/avibactam and tigecycline concentrations ST:STUDY_SUMMARY of 4 x MIC (minimum inhibitory concentration) as well as combinations of both ST:STUDY_SUMMARY antibiotics at 4 x MIC CZA – 4 x MIC TGC and growth controls as four ST:STUDY_SUMMARY replicates over 4 h with samples at 0, 2 and 4 h. Results: Antagonistic drug ST:STUDY_SUMMARY interactions between ceftazidime/avibactam and tigecycline were identified in ST:STUDY_SUMMARY the majority of tested strains. Time-kill assays confirmed antagonistic ST:STUDY_SUMMARY interactions, with tigecycline limiting ceftazidime/avibactam total killing. ST:STUDY_SUMMARY Metabolomic analyses of mono and combined drug exposure to bacteria revealed ST:STUDY_SUMMARY matching metabolomes in tigecycline alone and the combination with ST:STUDY_SUMMARY ceftazidime/avibactam, corroborating the identified antagonism between these ST:STUDY_SUMMARY drugs. ST:INSTITUTE European Molecular Biology Laboratory ST:DEPARTMENT EMBL Heidelberg ST:LAST_NAME Drotleff ST:FIRST_NAME Bernhard ST:ADDRESS Meyerhofstr. 1, Heidelberg, BW, 69117, Germany ST:EMAIL bernhard.drotleff@embl.de ST:PHONE none #SUBJECT SU:SUBJECT_TYPE Bacteria SU:SUBJECT_SPECIES Escherichia coli SU:TAXONOMY_ID 562 SU:GENOTYPE_STRAIN JUM_JEA #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t0_1 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t0_2 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t0_3 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t0_4 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg8_G_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t2_1 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t2_2 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t2_3 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg _G_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t2_4 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t4_1 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t4_2 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t4_3 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negG_t4_4 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_G_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negProcBlank_end Treatment:Blank | Timepoint:Blank | Sample source:Blank RAW_FILE_NAME(Raw file name)=Pellet_negProcBlank_end.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negProcBlank_start Treatment:Blank | Timepoint:Blank | Sample source:Blank RAW_FILE_NAME(Raw file name)=Pellet_negProcBlank_start.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC01 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC01.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC02 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC02.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC03 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC03.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC04 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC04.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC05 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC05.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC06 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC06.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC07 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC07.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC08 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC08.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC09 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC09.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC10 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC10.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC11 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC11.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC12 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC12.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC13 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC13.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negQC14 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_negQC14.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t0_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg9_X2_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t0_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_X2_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t0_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_X2_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t0_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_X2_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t2_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg_X2_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t2_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg8_X2_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t2_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg9_X2_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t2_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg _X2_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t4_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg%_X2_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t4_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg&_X2_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t4_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg'_X2_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX2_t4_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg8_X2_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t0_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg+_X3_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t0_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg,_X3_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t0_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg-_X3_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t0_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg._X3_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t2_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg1_X3_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t2_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg2_X3_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t2_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg3_X3_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t2_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg4_X3_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t4_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg9_X3_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t4_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg8_X3_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t4_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg9_X3_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX3_t4_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg:_X3_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t0_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg?_X4_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t0_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg8_X4_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t0_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg9_X4_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t0_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg80_X4_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t2_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg85_X4_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t2_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg86_X4_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t2_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg87_X4_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t2_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg88_X4_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t4_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg93_X4_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t4_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg94_X4_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t4_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg95_X4_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_negX4_t4_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_neg96_X4_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t0_1 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t0_2 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t0_3 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t0_4 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos8_G_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t2_1 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t2_2 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t2_3 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos _G_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t2_4 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t4_1 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t4_2 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t4_3 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posG_t4_4 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_G_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posProcBlank_end Treatment:Blank | Timepoint:Blank | Sample source:Blank RAW_FILE_NAME(Raw file name)=Pellet_posProcBlank_end.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posProcBlank_start Treatment:Blank | Timepoint:Blank | Sample source:Blank RAW_FILE_NAME(Raw file name)=Pellet_posProcBlank_start.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC01 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC01.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC02 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC02.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC03 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC03.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC04 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC04.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC05 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC05.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC06 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC06.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC07 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC07.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC08 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC08.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC09 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC09.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC10 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC10.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC11 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC11.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC12 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC12.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC13 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC13.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posQC14 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC RAW_FILE_NAME(Raw file name)=Pellet_posQC14.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t0_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos9_X2_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t0_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_X2_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t0_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_X2_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t0_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_X2_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t2_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos_X2_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t2_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos8_X2_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t2_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos9_X2_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t2_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos _X2_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t4_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos%_X2_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t4_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos&_X2_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t4_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos'_X2_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX2_t4_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos8_X2_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t0_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos+_X3_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t0_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos,_X3_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t0_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos-_X3_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t0_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos._X3_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t2_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos1_X3_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t2_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos2_X3_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t2_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos3_X3_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t2_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos4_X3_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t4_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos9_X3_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t4_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos8_X3_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t4_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos9_X3_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX3_t4_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos:_X3_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t0_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos?_X4_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t0_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos8_X4_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t0_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos9_X4_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t0_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos80_X4_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t2_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos85_X4_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t2_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos86_X4_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t2_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos87_X4_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t2_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos88_X4_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t4_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos93_X4_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t4_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos94_X4_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t4_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos95_X4_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Pellet_posX4_t4_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet RAW_FILE_NAME(Raw file name)=Pellet_pos96_X4_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t0_1 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t0_2 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t0_3 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t0_4 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg8_G_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t2_1 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t2_2 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t2_3 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg _G_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t2_4 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t4_1 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t4_2 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t4_3 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negG_t4_4 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_G_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negProcBlank_end Treatment:Blank | Timepoint:Blank | Sample source:Blank RAW_FILE_NAME(Raw file name)=Supernatant_negProcBlank_end.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negProcBlank_start Treatment:Blank | Timepoint:Blank | Sample source:Blank RAW_FILE_NAME(Raw file name)=Supernatant_negProcBlank_start.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC01 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC01.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC02 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC02.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC03 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC03.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC04 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC04.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC05 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC05.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC06 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC06.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC07 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC07.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC08 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC08.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC09 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC09.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC10 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC10.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC11 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC11.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC12 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC12.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC13 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC13.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negQC14 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_negQC14.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t0_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg9_X2_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t0_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_X2_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t0_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_X2_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t0_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_X2_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t2_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg_X2_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t2_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg8_X2_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t2_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg9_X2_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t2_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg _X2_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t4_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg%_X2_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t4_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg&_X2_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t4_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg'_X2_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX2_t4_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg8_X2_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t0_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg+_X3_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t0_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg,_X3_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t0_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg-_X3_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t0_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg._X3_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t2_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg1_X3_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t2_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg2_X3_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t2_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg3_X3_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t2_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg4_X3_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t4_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg9_X3_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t4_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg8_X3_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t4_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg9_X3_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX3_t4_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg:_X3_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t0_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg?_X4_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t0_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg8_X4_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t0_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg9_X4_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t0_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg80_X4_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t2_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg85_X4_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t2_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg86_X4_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t2_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg87_X4_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t2_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg88_X4_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t4_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg93_X4_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t4_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg94_X4_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t4_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg95_X4_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_negX4_t4_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_neg96_X4_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t0_1 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t0_2 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t0_3 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t0_4 Treatment:Control | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos8_G_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t2_1 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t2_2 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t2_3 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos _G_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t2_4 Treatment:Control | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t4_1 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t4_2 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t4_3 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posG_t4_4 Treatment:Control | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_G_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posProcBlank_end Treatment:Blank | Timepoint:Blank | Sample source:Blank RAW_FILE_NAME(Raw file name)=Supernatant_posProcBlank_end.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posProcBlank_start Treatment:Blank | Timepoint:Blank | Sample source:Blank RAW_FILE_NAME(Raw file name)=Supernatant_posProcBlank_start.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC01 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC01.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC02 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC02.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC03 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC03.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC04 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC04.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC05 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC05.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC06 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC06.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC07 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC07.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC08 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC08.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC09 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC09.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC10 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC10.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC11 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC11.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC12 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC12.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC13 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC13.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posQC14 Treatment:QC | Timepoint:QC | Sample source:E coli bacteria medium supernatant QC RAW_FILE_NAME(Raw file name)=Supernatant_posQC14.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t0_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos9_X2_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t0_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_X2_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t0_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_X2_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t0_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_X2_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t2_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos_X2_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t2_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos8_X2_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t2_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos9_X2_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t2_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos _X2_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t4_1 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos%_X2_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t4_2 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos&_X2_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t4_3 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos'_X2_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX2_t4_4 Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos8_X2_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t0_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos+_X3_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t0_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos,_X3_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t0_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos-_X3_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t0_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos._X3_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t2_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos1_X3_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t2_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos2_X3_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t2_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos3_X3_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t2_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos4_X3_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t4_1 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos9_X3_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t4_2 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos8_X3_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t4_3 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos9_X3_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX3_t4_4 Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos:_X3_t4_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t0_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos?_X4_t0_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t0_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos8_X4_t0_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t0_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos9_X4_t0_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t0_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos80_X4_t0_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t2_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos85_X4_t2_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t2_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos86_X4_t2_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t2_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos87_X4_t2_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t2_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos88_X4_t2_4.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t4_1 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos93_X4_t4_1.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t4_2 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos94_X4_t4_2.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t4_3 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos95_X4_t4_3.raw SUBJECT_SAMPLE_FACTORS E coli bacteria Supernatant_posX4_t4_4 Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria medium supernatant RAW_FILE_NAME(Raw file name)=Supernatant_pos96_X4_t4_4.raw #COLLECTION CO:COLLECTION_SUMMARY Time-kill assays were performed by inoculating and pre-incubating the bacterial CO:COLLECTION_SUMMARY isolate (approx. 1 x 10^6 CFU/mL) in ca-MHB for 2 h at 37 °C ambient air before CO:COLLECTION_SUMMARY adding ceftazidime/avibactam (CZA) and/or tigecycline (TGC). For bacterial cell CO:COLLECTION_SUMMARY pellet analysis, samples were quenched in an ethanol/dry ice bath for 30 s, CO:COLLECTION_SUMMARY centrifuged at 3,220 x g for 10 min to obtain cell pellets. After separating the CO:COLLECTION_SUMMARY supernatant (250 μL per sample), cell pellets were resuspended twice in 0.5 mL CO:COLLECTION_SUMMARY of 4 °C saline, centrifuged at 2,576 x g for 3 min, and stored at -80 °C until CO:COLLECTION_SUMMARY analysis. CO:SAMPLE_TYPE Bacterial cells #TREATMENT TR:TREATMENT_SUMMARY Mechanistical investigation of the PD interaction between TR:TREATMENT_SUMMARY ceftazidime/avibactam-tigecycline was studied in a selected clinical isolate of TR:TREATMENT_SUMMARY E. coli (strain JUM_JEA) using metabolomic analyses in mono- and combination TR:TREATMENT_SUMMARY treatment scenarios. Time-kill assays were conducted for CZA and TGC TR:TREATMENT_SUMMARY concentrations of 4 x MIC as well as combinations of both antibiotics at 4 x MIC TR:TREATMENT_SUMMARY CZA – 4 x MIC TGC and growth controls as four replicates over 4 h with samples TR:TREATMENT_SUMMARY at 0, 2 and 4 h. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY The methodology for sampling and preparation was adapted from the protocol SP:SAMPLEPREP_SUMMARY described by Han et al. [DOI: 10.1128/AAC.02656-17]. For liquid SP:SAMPLEPREP_SUMMARY chromatography-mass spectrometry (LC-MS/MS) analysis cell pellets were SP:SAMPLEPREP_SUMMARY resuspended in 300 µL of a cold chloroform/methanol/water extraction solvent SP:SAMPLEPREP_SUMMARY (1:3:0.9 v/v/v) augmented with isotope labelled amino acids (MSK-A2-1.2; SP:SAMPLEPREP_SUMMARY Cambridge Isotope Laboratories, MA, USA) as internal standards at a SP:SAMPLEPREP_SUMMARY concentration of 0.5% in the final sample for untargeted metabolomics.To SP:SAMPLEPREP_SUMMARY permeabilize the cells and facilitate the release of intracellular metabolites, SP:SAMPLEPREP_SUMMARY the resuspended mixtures were subjected to a freeze-thaw cycle. This process SP:SAMPLEPREP_SUMMARY involved freezing the mixture in liquid nitrogen followed by thawing on ice, SP:SAMPLEPREP_SUMMARY which was repeated three times. Subsequently, the mixture was maintained on ice SP:SAMPLEPREP_SUMMARY water for 15 min and vigorously resuspended using a vortex mixer. The obtained SP:SAMPLEPREP_SUMMARY samples as well as the extracellular supernatant samples were then centrifuged SP:SAMPLEPREP_SUMMARY with 300 µL of each sample at 14,000 x g and 4 °C for 10 min. A total of 50 SP:SAMPLEPREP_SUMMARY μL of the particle-free supernatant were then extracted via addition of 200 µL SP:SAMPLEPREP_SUMMARY methanol (including internal standard). Samples were thoroughly vortexed and SP:SAMPLEPREP_SUMMARY incubated for 20 min at -20 °C. After centrifugation for 10 min at 15,000 × g SP:SAMPLEPREP_SUMMARY and 4 °C with a 5415R microcentrifuge (Eppendorf, Hamburg, Germany), SP:SAMPLEPREP_SUMMARY supernatants were transferred and dried under a stream of nitrogen (Organomation SP:SAMPLEPREP_SUMMARY Microvap, MA, USA). Dried samples were reconstituted in 50 µL of 80% methanol SP:SAMPLEPREP_SUMMARY (v:v), vortexed, and centrifuged (see conditions described above). Ultimately, SP:SAMPLEPREP_SUMMARY samples were transferred to silanized glass vials and directly injected into the SP:SAMPLEPREP_SUMMARY analytical system. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_SUMMARY LC-MS/MS analysis was performed on a Vanquish UHPLC system coupled to an CH:CHROMATOGRAPHY_SUMMARY Orbitrap Exploris 240 high-resolution mass spectrometer (Thermo Fisher CH:CHROMATOGRAPHY_SUMMARY Scientific, MA, USA) in negative and positive ESI (electrospray ionization) CH:CHROMATOGRAPHY_SUMMARY mode. Chromatographic separation was carried out on an Atlantis Premier BEH CH:CHROMATOGRAPHY_SUMMARY Z-HILIC column (Waters, MA, USA; 2.1 mm x 100 mm, 1.7 µm) at a flow rate of CH:CHROMATOGRAPHY_SUMMARY 0.25 mL/min. The mobile phase consisted of water:acetonitrile (9:1, v/v; mobile CH:CHROMATOGRAPHY_SUMMARY phase A) and acetonitrile:water (9:1, v/v; mobile phase B), which were modified CH:CHROMATOGRAPHY_SUMMARY with a total buffer concentration of 10 mM ammonium acetate, when analysing in CH:CHROMATOGRAPHY_SUMMARY negative mode, and 10 mM ammonium formate, when analysing in positive mode. The CH:CHROMATOGRAPHY_SUMMARY aqueous portion of each mobile phase was pH-adjusted (negative mode: pH 9.0 via CH:CHROMATOGRAPHY_SUMMARY addition of ammonium hydroxide; positive mode: pH 3.0 via addition of formic CH:CHROMATOGRAPHY_SUMMARY acid). The following gradient (20 min total run time including re-equilibration) CH:CHROMATOGRAPHY_SUMMARY was applied (time [min]/%B): 0/95, 2/95, 14.5/60, 16/60, 16.5/95, 20/95. Column CH:CHROMATOGRAPHY_SUMMARY temperature was maintained at 40 °C, the autosampler was set to 4 °C and CH:CHROMATOGRAPHY_SUMMARY sample injection volume was 5 µL. CH:CHROMATOGRAPHY_TYPE HILIC CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Waters Atlantis Premier BEH Z-HILIC (2.1 mm x 100 mm, 1.7 µm) CH:SOLVENT_A 90% water/10% acetonitrile; 10 mM ammonium acetate CH:SOLVENT_B 90% acetonitrile/10% water; 10 mM ammonium acetate CH:FLOW_GRADIENT time [min]/%B: 0/95, 2/95, 14.5/60, 16/60, 16.5/95, 20/95 CH:FLOW_RATE 0.25mL/min CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Orbitrap Exploris 240 MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Analytes were recorded via a full scan with a mass resolving power of 120,000 MS:MS_COMMENTS over a mass range from 60 – 900 m/z (scan time: 100 ms, RF lens: 70%). To MS:MS_COMMENTS obtain MS/MS fragment spectra, data dependent acquisition was carried out MS:MS_COMMENTS (resolving power: 15,000; scan time: 22 ms; stepped collision energies [%]: MS:MS_COMMENTS 30/50/70; cycle time: 900 ms). Ion source parameters were set to the following MS:MS_COMMENTS values: spray voltage: -3500 V (negative mode), sheath gas: 30 psi, auxiliary MS:MS_COMMENTS gas: 5 psi, sweep gas: 0 psi, ion transfer tube temperature: 350 °C, vaporizer MS:MS_COMMENTS temperature: 300 °C. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak area MS_METABOLITE_DATA_START Samples Pellet_negProcBlank_end Pellet_negProcBlank_start Pellet_negG_t0_1 Pellet_negG_t0_2 Pellet_negG_t0_3 Pellet_negG_t0_4 Pellet_negG_t2_1 Pellet_negG_t2_2 Pellet_negG_t2_3 Pellet_negG_t2_4 Pellet_negG_t4_1 Pellet_negG_t4_2 Pellet_negG_t4_3 Pellet_negG_t4_4 Pellet_negQC04 Pellet_negQC05 Pellet_negQC06 Pellet_negQC07 Pellet_negQC08 Pellet_negQC09 Pellet_negQC10 Pellet_negQC11 Pellet_negQC12 Pellet_negQC13 Pellet_negQC14 Pellet_negX2_t0_1 Pellet_negX2_t0_2 Pellet_negX2_t0_3 Pellet_negX2_t0_4 Pellet_negX2_t2_1 Pellet_negX2_t2_2 Pellet_negX2_t2_3 Pellet_negX2_t2_4 Pellet_negX2_t4_1 Pellet_negX2_t4_2 Pellet_negX2_t4_3 Pellet_negX2_t4_4 Pellet_negX3_t0_1 Pellet_negX3_t0_2 Pellet_negX3_t0_3 Pellet_negX3_t0_4 Pellet_negX3_t2_1 Pellet_negX3_t2_2 Pellet_negX3_t2_3 Pellet_negX3_t2_4 Pellet_negX3_t4_1 Pellet_negX3_t4_2 Pellet_negX3_t4_3 Pellet_negX3_t4_4 Pellet_negX4_t0_1 Pellet_negX4_t0_2 Pellet_negX4_t0_3 Pellet_negX4_t0_4 Pellet_negX4_t2_1 Pellet_negX4_t2_2 Pellet_negX4_t2_3 Pellet_negX4_t2_4 Pellet_negX4_t4_1 Pellet_negX4_t4_2 Pellet_negX4_t4_3 Pellet_negX4_t4_4 Factors Treatment:Blank | Timepoint:Blank | Sample source:Blank Treatment:Blank | Timepoint:Blank | Sample source:Blank Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:Control | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:QC | Timepoint:QC | Sample source:E coli bacteria cell pellet QC Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 0MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:0MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:0h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:2h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Treatment:4MIC CZA - 4MIC TGC | Timepoint:4h | Sample source:E coli bacteria cell pellet Deoxyuridine* 0 0 13546 269970 204558 308231 163188 87110 462557 522244 195619 116409 98458 169108 190998 254113 305370 342172 309088 300751 242169 280961 257349 276123 273795 201104 288992 302305 310893 189067 89872 32933 77270 232857 589558 159109 335790 391726 314018 336270 131881 562187 473006 488763 548156 471030 785027 522224 730945 124334 67381 72030 311560 156655 157048 201399 314415 578647 494692 469714 462026 Sorbitol 37220 0 1710729 261352 218249 182344 67955 20898 51874 44576 26864 12868 17887 36609 256297 242905 268570 221581 295056 293055 250541 279421 346816 279056 327135 217610 322811 157927 144702 19331 23052 27409 13276 29591 18648 23748 16039 110259 69575 121763 228523 109451 107603 304171 140164 243650 498388 157099 121935 150635 56535 83229 134175 1070791 796197 636175 779028 476682 396132 1735481 246750 2,3-Dihydroxybenzoic acid 4024 0 2462267 3121497 2308658 4672888 345484 252096 195931 217726 67277 89915 68557 108444 477567 670030 806847 774880 844890 813805 869981 976127 868963 875917 757979 3618169 3226279 2635998 1532658 74754 78770 71680 87837 40518 66071 60424 65755 1658868 1599569 1887701 1357803 644290 689158 627414 797703 266507 349460 301670 382023 939129 1018976 1099513 654745 408561 470242 560355 451126 511065 363633 343692 413356 2-Aminobutanoic acid 82081 40484 427225 499539 491167 548644 476405 347356 429601 393596 508605 509336 471315 513906 463540 497050 520915 530511 508057 512394 461486 591508 480909 523794 508153 541028 566730 372990 378641 215101 232207 151418 122536 291960 391891 445845 500640 681523 567678 752470 731345 828262 891798 1209080 1516006 473951 574349 557669 1193420 414729 449992 585600 414265 721567 957140 945511 936454 586888 442078 910652 528037 2-Hydroxyisocaproic acid 68316 62978 1002926 1204307 1455000 1343895 2548163 2094404 1705114 1785974 1003366 1290294 1035757 1446178 1341720 1440634 1577725 1536163 1863550 1911814 1963092 2064200 2255277 2124865 2168137 887499 914692 724582 602964 3006153 5464098 7785050 7347734 3348351 2834544 2704124 3095374 1205923 959826 1109002 1398921 1418065 1569702 1364054 1464927 993038 1577608 1236317 2803286 720321 851811 996863 658153 855339 1471950 1258704 986656 912187 1086513 897453 1427068 2-Ketobutyric acid 82556 193864 235441 182893 206626 217269 646589 603458 498485 719961 201263 190406 201968 185941 511362 570448 589387 529206 590285 584954 528410 540771 528042 490796 529075 288111 226139 193306 294797 638488 663802 423650 560481 348589 497793 248827 320236 666775 453356 482069 374959 1191863 1405491 1963646 2027598 1167876 1482513 1540774 2503764 189757 258184 230712 276838 501496 612371 651691 561341 383753 399355 719512 462557 2-Phosphoglyceric acid 59216 10485 2415489 2132647 2750761 2627958 3052597 2452199 2783965 3411997 1943159 2090143 1665613 1979500 1985225 2211517 2180909 2173996 2101614 2134758 2051519 2076307 1970428 2039296 2010341 1631953 2088843 1822136 2299715 1719515 826708 86081 139960 348648 1200882 1564504 1544198 2770559 2170408 2954005 4652666 1762514 1813244 1657517 2153992 943704 910295 926627 1878089 2697971 2842760 3322988 2072935 1781628 1993518 2039441 2585146 1852098 1426636 1627423 1702978 3-Phenyllactic acid 58682 29273 687544 776307 898426 787977 1244482 1104916 969885 1235933 668695 852912 741912 894805 682418 755771 858406 918524 986357 1023691 986242 1114911 1233372 1157084 1163928 670188 722329 539897 558338 1646886 3800149 6359712 7319960 2143355 1637293 1453314 2014692 800005 600106 627050 710124 585955 683015 638019 752484 491739 557531 721325 1055877 558831 651017 823034 584265 680734 998246 801780 661789 654999 659861 627837 923635 3-Phosphoglyceric Acid 64546 6633 1871642 2058391 3035658 2795386 3943685 2517654 2442435 4490938 406237 1416809 226602 225905 140710 1416870 1486129 1683737 1523090 1838458 1970874 1894018 2052828 2215740 2218987 347728 2323640 1472839 759448 1390752 91090 67532 73083 85614 937206 1405435 988014 2414816 3297250 6117639 11511306 1335732 1839581 102205 2067398 180268 125107 916460 2122681 3663008 4336730 6856884 3206276 283784 2192202 2009704 2832853 1519506 1043130 1645127 1326519 3-Uridine Monophosphate 10969 6544 6819924 7234539 9929774 7628224 6598368 6208652 4534206 4761502 8101735 13340841 14381536 13830099 8619618 8835224 8973931 9231266 9213824 9232392 9052195 9045191 9293106 8946683 9220777 10288280 12845539 10567794 10420695 5285446 2732634 223884 442184 2252916 5320813 8678073 6570208 17435990 15254835 18180000 25255728 7903968 9851767 9049720 8932397 6118064 5822724 6894216 9524030 13612552 18166320 20349602 9868720 7939733 8754451 9140338 9346608 7581012 4498099 4156954 6506267 Adenosine Cyclophosphate 0 0 135106 87778 110178 98762 74913 90406 145272 108577 190750 238176 207340 195817 303634 294854 320504 302262 290074 301685 267948 304154 311322 279772 273967 90199 91860 60570 81659 934770 1087533 316024 433885 410711 412245 569417 496924 107174 97881 102795 72593 348068 254804 411047 243147 344314 644084 321037 444265 127553 110586 142980 221359 218853 216369 241611 226900 526182 703160 717913 596687 Adenosine diphosphate 36617 31876 21940762 23767134 29498610 25156924 21719198 16305925 15326583 14435374 10601868 12272058 9860691 12275878 19586940 21553628 20749614 21473192 22017376 22000762 19618016 19619994 20733312 19529282 20280714 17463132 24398840 18470552 21767542 9772655 4140094 195433 356138 1748702 7363910 10620462 8854323 35126604 26796660 36471408 41848488 22313342 24019598 23987712 24029500 12752064 14481767 12691741 21520914 32302292 41880272 44929392 35756864 24116756 28068876 27411062 30580184 20893448 12462690 11030797 16286660 Adenosine diphosphate ribose* 10457 5825 60353 63656 89958 96873 85881 68584 64837 82345 62695 84743 72511 77136 71980 72572 78676 80639 81140 76248 82882 96427 69819 83973 90945 51043 73247 62133 53833 81320 45173 4628 12394 22941 61623 61760 52810 54573 76628 78465 81252 74546 74540 54754 89605 58868 73187 65503 91942 76989 77153 73756 80050 77206 115145 109379 80801 94640 79357 68710 98014 Adenosine monophosphate 36106 10269 32588374 38805360 53154948 44469160 27817610 21194126 14910484 13904953 14925754 23177070 19808712 20224420 30769030 31360590 31758470 33397410 33266596 33435864 33336078 33358574 33792212 32801866 33356042 34526596 42188220 36548308 34002496 18736932 10779267 1314822 2259437 7335466 15149286 26622864 18237176 46986232 42205100 50064348 66999652 37998512 46667704 39882004 43832408 23453334 26962388 21849632 38221996 52134816 69167480 70206312 37949116 39176432 41257004 44872016 43309152 35162504 22780912 22551970 32010146 Adenosine triphosphate 15951 5486 5958968 6798663 7990735 7613276 6345736 5034448 4546200 5574354 2614610 2784312 2234745 2200126 4133331 3918286 4357664 4274420 4361145 4316090 4564167 4683110 4425469 4391318 4606658 4137186 5600184 4981068 4763172 1823909 725425 47642 71760 354349 1324475 1226011 1475974 6197103 5142572 7351917 6576412 4052854 3832580 3419116 3735522 2423132 2621243 3052498 3716830 6453790 6543940 6796515 10504989 3547658 6296788 4750748 5253387 3748680 2516190 2273551 2935674 alpha-Ketoisovaleric acid 118961 295722 2198697 2657868 2430829 2543060 1424592 1373558 1587729 1531561 1037646 1086009 932171 1046755 2344017 2658436 2744016 2758824 2775846 2784753 2869158 2906734 2996902 2878832 2863667 2479380 2028084 1591759 1977195 1696698 1932627 666688 951865 1164184 1110598 1282107 1547413 4536370 3336268 4003574 3708729 5586570 5457138 6142420 6543170 5240842 6196806 4860397 9010925 2019987 2701214 3408619 1538075 2964486 3740120 3230522 3163126 2545994 3256367 3022594 3328390 Aminoadipic acid 9525 6947 717549 738164 783817 918565 72002 28623 47826 49918 114087 204319 132651 103589 295184 255236 266796 240563 253488 257802 286258 271532 262412 267377 248313 378038 438356 484744 380826 14201 24701 5270 11013 20645 21416 34970 25110 831785 907544 1082028 666790 378117 394855 385195 444640 231709 256280 225889 291436 152955 163266 145424 77660 168942 192639 210998 204088 110353 81941 87702 64323 Aspartic acid 24754 62862 2085431 2844662 3323908 3401797 2981729 2925980 2551155 2611423 2809271 4309596 6735721 7419272 1966129 2306734 2500520 2736790 2738138 2843885 2768156 2773222 2840933 2943346 2888321 3078649 3268336 2451186 1748090 2843047 1114206 92543 164622 780595 4991352 5672730 5362738 2233972 2353057 2897381 3896265 1487735 1972304 1510688 2255274 1247506 1156207 2483720 2406947 2021663 2132402 2146092 2005727 1362527 1636865 1736255 2025149 1267649 1215031 1583444 1228933 Azelaic acid 204089 158397 1296810 201403 184922 203985 175379 180075 175903 196847 195179 170155 181907 162868 227675 231131 231422 232297 259359 235760 225199 241284 227532 249849 257223 239114 174499 156930 178635 160665 459020 2494447 1648589 555261 157321 158532 189344 178488 200679 174305 294179 165496 175981 159118 167235 186704 179988 361729 256178 169097 172007 191383 159943 149781 200170 180602 159471 163636 191551 87242 180248 cis-Aconitic acid 67130 194762 5179212 4322046 4124276 4224510 2555530 1785487 2858396 2193228 1419021 1509428 980932 1805618 2531678 3432750 2537340 3464949 3389592 3245482 2379870 2458098 2396643 2442446 2445032 2984141 3435836 2976054 4754882 158534 143925 84250 110386 146806 141949 216418 258504 3067478 2478402 2935056 4660400 3452426 3590952 3830085 5125163 3788523 1995206 2411030 5518707 2194325 1991396 3583784 1570898 3338496 3032497 4235271 6300836 2612326 2782461 1891412 2388699 Citraconic acid 117410 47303 2906182 308901 365488 405669 348716 348526 366426 362241 302008 373660 263488 288021 547663 639403 720702 694528 736904 749965 801946 747972 762566 736011 701250 299017 383364 196065 262171 240095 236765 105956 127937 176743 226711 302766 236846 366906 333030 327042 1599316 216956 309538 191331 242867 487973 297708 640716 1207114 312655 252054 258826 202204 257428 357987 258653 230497 234143 181263 223521 380851 Citramalic acid* 45538 35915 19941546 22416350 24400808 15092481 1953617 1278089 1813267 2095677 5148536 5608703 6098940 6656850 13120289 14788763 14068440 14265974 14343421 13866120 12171847 11735793 12250779 11622672 11506351 20437444 14579124 19523328 18608104 411532 393294 63449 103439 460793 1590833 1368130 1386487 35077856 21112190 23160564 21209628 28030320 26884090 35100016 31798878 9921425 13007678 11331969 21644710 12851142 14983114 11720366 11864559 16855684 17213650 15815931 16683275 11049528 7336766 4974321 8533742 Cytidine 0 0 48109 79819 67902 102185 111261 100420 269297 207681 214624 309290 430511 359782 240751 244666 260995 244411 265145 283870 253197 241803 244449 237595 278379 87107 112695 43055 84261 206170 234896 124715 269558 210195 272579 199249 251318 1293332 900050 1080079 440558 69033 47930 206100 130152 33454 75165 150920 101710 263508 321403 403190 1387057 169129 241901 138649 159465 155729 111699 104057 99427 Cytidine 5-monophosphate 6027 6744 3782642 3496982 3523812 3065182 2470292 1735670 1498029 1566687 1892452 3335102 4421688 3365998 8944914 9580841 9471080 9507533 9446082 9820935 9347025 9522832 9587564 9116874 9501952 4211508 5145866 4803918 5155222 1058682 592113 71200 122222 463105 1095701 1710758 1306233 22045390 18485092 22501886 28377780 13648121 15854660 15133465 14761378 10717463 11219825 11527719 17492914 19469166 22139448 23522436 16594473 15073271 17241052 17244098 18109108 15782796 10072092 9278678 13111987 Cytidine diphosphate 17461 0 1182623 1222621 1265321 1133660 1095137 873379 795464 911841 730621 861543 849612 805235 2860361 3172293 3194374 3168111 3191255 3175730 3201345 3152380 3091898 3096291 3189806 1154073 1617482 1374021 1551685 293870 161417 40203 37096 92515 376392 477933 294067 6760958 5115468 6780142 8318249 5433378 5500304 4702474 4808980 3909972 4264229 4687214 7502987 6360028 6987344 7508332 7531847 5146481 6915230 6121172 7783196 5667356 3446808 2880088 4394374 Cytidine triphosphate 3506 0 311643 374916 421088 374152 380155 292300 254601 300144 211101 239281 235822 193191 475515 462361 485693 508320 490130 492790 517787 514815 486362 478791 473059 305697 405515 399626 336217 71615 37504 15440 7571 25973 62216 93791 61279 842280 588092 899304 836359 632276 576325 502950 475249 497905 606268 800304 855088 867009 805628 758660 1546931 573998 1073918 783713 894747 756667 463714 323773 535964 Deoxyadenosine monophosphate 0 0 251639 302309 391532 381787 250251 223123 214171 205414 246485 364349 382932 363705 356575 397063 385774 415210 392965 400786 403622 362114 386090 412930 434632 251303 287308 211231 198560 170289 116318 21517 39054 76342 134347 200905 180769 259211 229971 304153 311779 1133710 1284796 1276687 1492733 551010 621307 523559 877878 243445 328041 349398 193219 480309 475617 518913 477153 589828 497140 383685 515586 Deoxyguanosine monophosphate* 15929 6201 302993 262198 312832 255642 200446 179932 180365 150825 236527 312664 353863 288361 381561 338968 322815 354959 378603 332271 316233 347741 306112 310751 303150 308856 308143 284334 325049 153671 105122 27762 34781 75236 123829 225928 168791 576422 392609 439865 611934 394733 376553 460298 415055 214721 262695 255359 356453 510955 567862 562325 425914 476004 456869 490534 473489 413717 282295 246489 398861 Deoxyinosine 0 4405 186006 125169 84768 122352 182443 67495 155959 178122 101162 41490 23642 47845 182068 204966 184009 183481 188059 195702 191926 199357 189133 216565 207318 89449 97084 89256 148209 41284 33220 44050 78731 116878 118869 48159 97778 116647 69139 100704 86846 443399 329070 457765 404492 303983 496603 406609 737157 111550 66164 99057 114686 180954 188775 200032 249844 402596 433724 442253 360508 Dihydroorotic acid* 8717 0 186686 97602 123828 158566 2525 3525 4348 4834 14885 4706 3260 9029 23003 47665 14002 56743 56639 71470 53420 65077 73232 124246 94740 11061 4810 8421 1797 8159 8038 7074 2253 9315 1474 2853 969 22378 14591 2757 57852 215596 201210 222164 7685 6675 1201 18755 27919 4985 14130 6627 14860 3425 55576 134535 122090 8128 2462 8928 9137 Fructose-1,6-diphosphate 38391 6920 503290 465729 646837 589748 230240 215696 198750 236282 145677 119076 123037 107280 309725 343934 416998 362850 349628 382442 376378 372052 367117 389975 360312 375211 485777 536571 423299 107508 91717 45606 21248 35368 105847 138267 105576 607114 629371 845405 421171 321845 390769 527463 340244 335636 668670 569348 441905 508123 694733 564135 613172 558242 639395 706036 373520 453837 315051 376323 411710 Fructose-6-phosphate 0 0 931440 1005923 1207507 1351520 1066002 863113 1144054 591454 355122 470230 326535 498695 544460 698009 570065 760211 771795 701985 545421 541389 574190 542229 575471 673058 851504 689777 600961 267602 139824 7014 15953 68076 245661 358737 386039 681419 520318 639253 1177240 417538 419590 447958 669711 298715 290887 183204 530891 626076 469187 651803 461346 635973 437643 574898 671666 504599 360668 620505 333038 Fumaric acid 45181 32243 5356451 4480654 3761471 4473386 5304962 3719841 8513999 8501947 5677818 3227708 2432381 4537872 5418352 5517826 5601218 5564413 5507704 5524330 5255215 5246346 5400935 5140776 5252680 4134372 4395828 3626816 5333888 7279948 5101297 852891 2029398 3051844 9532098 4718011 7116634 8688689 6530656 6392540 6876534 3168796 2948068 3664104 3795941 3220742 2048956 11470885 4424529 4528056 4706674 5335734 7347223 3845308 3945260 3920862 6096496 3978069 3885639 5868638 4028810 Glucosamine 6-phosphate 3578 0 1022500 823953 1055380 1147001 1172136 838303 892034 948796 468881 415877 369526 415520 706206 759329 779171 710958 743505 746228 728970 763829 763942 793973 757636 856320 1151760 780713 1033120 848907 345517 31426 54274 140467 414181 569219 490702 674831 500666 619749 702695 373679 303345 293930 328979 173398 262581 253959 424178 1102201 1116470 1191930 1089680 463064 555031 457846 637534 863183 724217 612952 821335 Glucose-6-phosphate 0 0 2398353 3172773 3652028 3789324 2953410 2618818 1963972 1299986 977130 1175157 1095433 1095096 1645746 1789098 1676559 1763629 1795426 1729345 1643438 1710347 1660547 1681122 1637486 2456832 2504511 2246064 1857194 655575 440603 18550 57993 187183 611324 1039434 698053 1937374 1656528 1988648 3074228 1461649 1382667 1141738 1673195 1046368 906808 626163 1396732 1508820 1233560 1351216 1084659 1308613 1248784 1239670 1455038 1301034 897384 1130299 893542 Glutamic acid 28196 41306 154740096 203165504 207084208 199333056 77796168 50253860 52468808 45296916 115304544 119098776 119739096 127572152 135791840 145766912 147436416 143087088 149690192 143828384 139934656 140155552 138914240 135976208 134174944 216255168 211943760 180917328 187846272 10046939 9675384 558991 1542563 19126518 55417228 41275548 42307484 318390528 260128928 317564000 289286240 214503520 225626208 243686224 238001392 167761440 163301824 194484768 234091488 139898800 151496320 138624080 126268192 125974784 149851792 158990640 159221376 111282192 75196464 88623864 78258272 Glutathione (GSH) 1211301 185290 116305192 146883296 217446496 222968096 229453824 143795840 160892080 158385952 178652544 219446992 198717152 230056336 129338920 140714496 147971152 150901776 156152960 154632688 145944256 148282928 150694320 142555056 140909328 146037904 145569648 125195152 128759512 115959488 61621584 5962905 11962785 32684914 68099872 124776096 102714352 155337776 144469424 192418288 220852400 93539304 129695008 115017192 155110096 68488368 64003796 49976096 92708968 125366872 141760768 157929472 118628464 161583568 215092368 211367776 184844112 292825216 159930944 78671968 214527568 Glutathione (GSSG) 3720 0 663884 701061 1284531 1337951 1196986 776670 857381 1053437 915990 1296102 1179434 1385578 664746 750460 765735 830429 803866 893446 885107 950204 1002362 1057974 1075858 626448 837026 515298 517008 593653 351523 28186 68735 139563 381770 640835 583090 773374 722311 1127210 1580376 301826 479380 368347 532434 262317 211543 290903 612528 622510 711634 778505 939948 630192 1067327 909251 764653 991947 606598 351186 849726 Glyceric acid* 8471 0 368345 720693 889485 766813 679670 443552 325003 505230 232176 492397 412086 387002 258880 423292 497524 518441 510936 495586 968216 689829 921813 1069121 1021227 450620 1000182 737682 322968 528139 278895 48798 45735 87742 566539 546922 563356 332698 842672 990153 523471 433722 781908 233695 473536 452919 348157 908473 1180473 756277 534444 734268 908008 442807 1005143 857454 581637 593144 248536 786411 353391 Glycerol 3-Phosphate 0 0 549511 1072848 1250186 948225 271741 172328 511393 499513 380430 398928 335120 426710 935310 945957 947299 997292 1048193 961327 1033383 909550 968857 984417 932108 669243 636761 682993 292906 731402 389766 20392 29226 124427 562098 380349 445776 982095 831581 827136 739684 1724142 1969978 1928606 1768420 958950 1836334 664095 1833548 1174485 1631465 1511724 1131395 2590855 1885569 2068656 1506944 1352887 1257772 1620373 1997077 Glycine 118314 117029 1433234 1646090 1175290 1366540 226003 202858 261830 316397 193459 228739 233150 300540 971233 1098593 1030186 1152664 1066396 1059459 1068532 1136262 1143992 1092208 1171277 1147556 1325639 954302 1139561 505629 413370 131689 191660 334151 1141923 416389 484576 1913880 1359494 1911121 1244603 1113752 1533315 1624280 1845940 1550001 1468198 3346992 2711315 929407 1009871 1139034 1221779 1289702 1644520 1882768 1751134 1703812 1896852 1977236 1923218 Guanosine diphosphate 13852 0 1140046 1111718 1170208 1152297 664005 551399 453227 502550 495912 633920 672511 581657 1854864 1927234 1961578 1971859 1989422 1932350 1948430 1979965 1952188 1992970 1915824 820971 1014025 864720 845796 320407 150206 42280 43044 77979 274591 355287 238329 3445416 2636405 3474578 4575762 2820350 3108198 2593130 2827666 2200828 2234496 2391734 3435366 3486510 4017132 3973600 4610498 3599808 4558136 4299321 4739800 3713576 2520823 2320289 3322063 Guanosine monophosphate 5915 5836 1099878 926981 1189904 1056344 556951 395096 373253 437549 487432 756238 830406 615726 3358126 3411372 3482094 3674480 3612547 3723988 3569687 3605417 3688252 3756874 3623602 1034511 1305046 1082255 1068030 524597 247660 20166 36635 115340 351083 428132 423727 6337204 5583881 7077780 10720715 5895480 7188180 6280976 6877432 4495622 5145224 4967754 7722120 6503972 8380698 9284307 5569873 8384072 9085860 9634082 9347036 7885928 5245564 5261494 7404962 Heptadecanoic acid* 2466098 2336876 50065956 35308840 37890844 33936536 45114596 55310756 56678592 78505056 49456456 51023924 54726452 55051412 59801140 61851492 61249780 66549920 64752724 60859812 68088384 64902128 65679172 65673876 65010176 86503376 70980960 43546932 62115948 76068768 171745904 153550880 147604416 202911376 86529776 94291640 138901264 71116640 54849936 44109180 41686292 37689540 43646972 46021208 60327900 62398032 46768408 5579472 45589208 53004044 51407308 58963748 27241328 70598264 83940528 79358872 52015640 49155852 56069128 51757740 70999728 Heptanoic acid* 7195904 3874666 6300803 7080802 8046673 7720612 5427782 5674476 5922739 9726591 6893555 7467834 5630051 6189418 7196172 7491050 7660391 7861752 8209922 9181467 10010375 10362974 11133510 11422653 10882705 6497374 7828719 7891739 6143310 9016855 13288891 24802662 25897300 16826106 7417318 10140094 8615936 6220258 8621724 8312460 8924344 7461978 5889591 6362940 8591778 6414834 9006587 5687002 17798778 6657554 7432706 7916068 6535449 6974538 9380643 9186941 8897247 6335660 7464876 6737097 6843486 Hexose-phosphate 0 0 1117519 1181327 1304587 1449844 1281409 1070221 812033 589533 394575 474286 415576 448498 567938 639044 647112 661856 638418 640113 641964 647320 625529 618210 645126 905915 925995 804792 684801 315895 202053 10362 31543 65481 247984 369595 282300 708242 582927 751645 743085 444348 430751 437034 513405 329820 316338 226260 437034 600233 440465 486260 420951 402221 460214 418590 498642 439739 387731 410572 298827 Indole-3-Pyruvic Acid* 2805 1133 640 0 5247 7384 109866 90464 90180 129895 38305 21890 22061 26919 41278 51185 45670 35383 50454 45911 45400 47608 53607 46112 32601 394 0 3880 7219 182220 191725 99357 113491 71657 52656 105766 106431 14704 5722 20692 6255 11670 14197 7544 11766 10780 8366 21971 17331 25962 30511 31723 42289 16087 19995 17043 12375 16480 19954 24669 35649 Inosine 230911 253624 9468046 7483660 5967639 8200069 8228808 4205414 9383474 7549572 6457469 4517756 3239049 5305583 13180545 13748980 15166023 15525405 15170196 14564180 14450713 14112774 14362839 14343288 14126302 6937132 8007788 7598702 9107678 4836117 3683431 3278205 5964217 14212682 14092488 7478677 13671945 22467082 15419704 23117856 20338064 19107480 17351322 17511624 18002308 10788928 15183386 11412735 27623888 24382714 14725575 18961134 18600402 16427839 20441464 20448414 29319884 27002936 25063558 27267190 23382486 Inosine monophosphate 2402 0 268668 349989 454941 362050 15278 6234 40222 31972 82564 130183 156968 94361 1057456 1176486 1197298 1189433 1188732 1184287 1211011 1125578 1189962 1208006 1157689 578336 855403 712182 520653 29752 3736 1452 5057 5294 19420 12800 10416 3058770 2773688 3107238 4735128 1720018 2059572 1897578 2293112 1303038 1559339 1214145 2401760 2693791 3208660 3350514 2025482 1991135 2307900 2017205 2257476 2105415 1103429 670759 1424556 Ketoglutaric acid 4992 0 5599498 5765270 6576567 4788303 1838218 1379798 1266356 1090822 1109554 1061820 1231248 1171810 3737132 4401244 4162795 4068164 4105064 3866286 3608261 3445491 3552430 3483768 3335526 4954512 4660218 4554944 4975125 168087 153019 14582 31267 164508 418005 297262 374329 16649908 12392405 14253612 10370167 11584283 13882132 21810342 14386938 13701815 13776532 7136266 11962151 3720772 5454063 5283016 2985239 5074078 8364900 7668854 4462081 2238284 1465799 1331156 1596046 Lactic Acid* 1401411 2133110 2428880 2747591 2750298 5058787 3090124 2872580 2692237 2844112 2095458 2775026 2149049 2690878 4635800 5031576 5047871 5664512 6031514 6496325 6824846 7095562 7380807 8034308 7790096 2856643 3735369 2815929 2310550 3524332 2870412 1278089 1358478 1825705 4149778 2046141 2492960 3216676 3026828 3691861 28993206 6677718 8938961 6960451 6263431 6884810 13173310 10055333 36306764 3193158 2449340 2615627 2948876 4691614 6549460 6579846 5670834 4576858 5225518 6275192 9217077 Linolenic acid* 125869 151771 21710934 30860390 26374484 25546574 640093 1089524 811206 778540 301569 491760 426596 679985 7685366 7639654 7425498 8700821 8328855 8917985 8938019 9183521 8384734 8717040 8679916 27582098 17854038 17695786 13641931 2985458 6467825 8431316 6647149 6130055 4326687 2386926 3305192 10304005 11988762 14047706 11312781 12799118 12132400 12652221 9728920 6066608 11467202 171911 10181490 15902350 21076376 17942032 1225743 8268027 6866860 7742405 6119971 4692602 7398554 7704021 6191216 LPC 14:1* 0 0 2596074 2251282 2405842 2273212 1264688 1783530 3244416 2881775 3248539 3666987 3497447 2702040 7272093 7105196 7239928 7517358 6954108 7936334 7438606 7750556 7974324 7889538 8135686 1742007 1788422 1933430 2051880 19444720 27659882 28499946 40154352 74184992 71744528 40689264 47085788 1974026 1932832 1786504 1460569 2545902 2548135 2503200 2002432 2140951 3161019 5904175 2713281 1479923 1792400 1508488 2486397 4211939 3095517 2901038 3254195 2769453 3641274 3820030 4559256 Maleic acid 511579 86514 2410121 729659 708078 660511 606542 562727 650786 795047 530231 765505 431318 612154 884444 1020937 1103789 1145960 1199852 1208679 1390742 1258941 1292969 1267154 1249008 568124 738849 527908 732219 626689 504228 211509 247079 393967 635734 866075 571742 714297 639449 720377 3140478 664931 691801 639612 797404 672141 620139 797463 2496317 671133 625728 691141 597425 651639 829124 814236 926393 590384 558083 649353 772608 Methylmalonic acid 124485 54637 20897706 25468022 27809264 22157178 26114492 17728144 22539090 22402962 16727691 23030686 27030494 20002352 17348036 28183336 28171058 27410222 26504306 19171008 43602748 28193002 31601392 37558444 29264950 28714292 27108192 32662800 19850740 21070870 13971541 977486 1347735 4759238 26614414 21041306 31671034 21531496 29075314 34979636 24198152 22165218 25949710 18886794 17740744 25227334 11174995 42221628 35763676 30343340 18823672 30696818 38779888 24767192 40540944 35293388 27166768 30333310 7601584 21744738 14152797 Myoinositol 6544 11273 1251370 739188 1152369 1196579 1301604 1029271 1079758 1164760 278875 428166 501185 632985 487877 633416 688829 753678 751052 888811 815259 854642 880224 988641 967737 556666 1015572 592090 536663 1019241 596549 21059 233170 300706 1043361 987598 757275 562099 732784 1020091 1063418 390369 602617 343513 810405 371538 439733 702171 983003 427055 485605 481077 742661 501671 947615 827473 789328 886560 502765 596923 717366 N-Acetyl-aspartic acid 3326 0 366746 251816 181388 329342 1416298 6126602 12407189 15310518 1842790 1869267 1973353 2075227 1522418 1529145 1503342 1330584 1388070 1357575 1253303 1252977 1135244 1149712 1053234 313697 334357 447420 460155 871615 943531 229770 432078 587400 855732 1037102 1081494 2384438 1996387 2694154 3309116 4051213 5537544 7932056 5859624 287940 208737 136926 198425 3234950 6686734 7833946 3156540 1697569 1647664 1392967 1379539 1414469 806630 493210 1182406 N-Acetylglutamine* 0 0 891942 530543 601801 570709 775751 535258 311436 624802 291921 286368 231309 308465 461905 436482 389375 436997 569650 564888 447444 408652 402876 416719 422870 192240 588166 637603 363985 242138 11293 2345 14731 355 474758 350474 212393 808592 445272 601045 583116 272121 230795 344278 305573 159944 181952 221233 396719 286308 170966 238051 213946 203075 338423 411434 382211 316712 178117 178614 276194 N-Acetyl-leucine 27145 39187 728895 760815 864216 884817 568588 512846 560084 552285 478439 619609 672687 808201 490375 541506 538923 548159 566442 572524 553677 608677 545754 556088 565535 877420 685260 537642 573768 684208 1197089 2353008 2235912 1382252 683294 748035 862493 779505 615756 809536 799073 380495 452906 513346 494244 421622 463498 628048 779977 317676 359848 439542 279397 424376 396175 385414 407849 325450 252031 297795 340928 N-Acetylmethionine 8751 83854 497638 530544 731427 736072 151234 74215 96812 80582 88852 72950 108150 112533 263541 304686 316225 346509 326961 332486 321108 325798 334356 331087 312705 938264 648674 547104 559245 149294 288847 381360 333731 301549 249414 187567 231067 970172 715309 721414 697687 153778 218985 232401 301087 208118 206817 297171 413934 497553 229717 309639 337981 152263 146936 182312 172225 159197 163612 181919 147195 N-Acetylneuraminic acid* 0 0 101634 130154 157913 143023 146150 95968 113035 97231 29289 34521 34355 38759 121810 144752 130822 134947 130043 158342 148514 146266 148735 158066 160622 104098 145254 131765 109248 111044 55220 3906 0 12927 32661 51305 36237 225967 215845 259990 280401 178851 210435 180410 245193 104517 146906 101596 219617 166313 239865 223052 183362 164326 261392 215755 187653 230989 178523 105350 209829 NADH* 0 0 302596 241761 477404 448860 91389 43205 210278 292908 315870 744116 894892 739864 232685 219987 221847 245320 225508 212054 203966 199430 186744 174535 178714 218083 310088 267936 488011 65341 398381 24242 40453 41610 145322 644837 138032 629420 315648 669927 127211 36532 46774 375646 76614 70210 323536 259953 228688 96008 265105 224178 260095 276030 289366 278790 82252 320890 179929 20685 292138 NADP 7792 0 1153368 1340837 1614822 1554754 2620188 2426935 2280649 2480647 1707608 1991531 1749871 1966212 1629693 1716333 1898219 1869470 1905974 1868685 1863038 1951177 1886424 1928564 1957906 1013514 1411252 1141525 1071051 2636999 1164885 47946 99513 482188 2073662 2715568 2189404 1571379 1357492 1743128 1847762 1756549 1882019 1632691 1931033 1149508 1682409 1401171 2550566 1647855 1760718 1923511 1610304 1288408 1590254 1637100 1523166 1822571 1566434 1004180 1650772 Oleic acid* 7032051 7494440 207852560 273894304 259493680 255347664 54205572 59814168 68407080 79032592 70191064 62137980 60271184 71207760 290247712 285816032 299623040 300175488 310655552 291015648 310233792 300976128 306526432 310969664 311823872 379127744 261911520 176431264 183926336 345482816 758479808 645251264 614767488 1072181312 725029440 457818784 628027200 135472128 155421328 166280768 167002240 537842880 512464000 566270976 492703392 463461440 627827392 14417480 557874624 262171760 323130272 266780256 38013440 634312704 530552896 626451776 569734528 444467424 577408128 772298496 647495552 Orotic acid 747104 857999 1601046 1308523 1700157 1726851 873040 784970 849267 850814 833668 909132 854614 822426 1396152 1496503 1639237 1573408 1649394 1547248 1477890 1512869 1499714 1426756 1388886 825587 818412 839271 850098 798887 802892 813359 782132 871280 919502 761603 844632 2338750 1746765 2387598 2541472 3658520 3552150 2914396 3104176 1029249 929267 969199 928980 2528822 1802307 1759476 4847791 1886162 2294855 2960630 2323002 1382569 878167 930478 899236 Ortophosphate* 325988 257847 45180784 45586000 58901468 52900940 46974852 38887104 39239804 45136940 31180660 41568020 40275688 40929492 38067004 39805140 39282544 41375116 40224892 40743588 41009576 41241736 40910332 40777020 40158344 34080968 46084296 38181436 41554572 40228092 18150206 999123 1626055 6805712 27204728 35200348 29018570 56374148 44107072 58984556 69344168 31071878 35147724 33413862 36569280 16209356 20890978 21500180 33078788 58860900 66536616 75312112 61893796 35384840 41508748 39775136 41904576 32895256 22243842 22497524 28399900 Palmitic acid* 148163776 119505504 620466240 937998656 777044480 812043968 641403072 548231040 701148544 772170752 819943488 532609920 488362912 632431424 992198400 1010526784 1013666624 1065774656 1052419904 1034002816 1132143488 1095874560 1099849856 1139926144 1149615488 1411695616 1172402432 679681856 952702272 1022059456 2587976192 2790702336 2160154112 2804313856 1530537088 1476512128 2006945664 551618304 705698432 659272064 1165035136 988228544 1072024768 979051840 675832192 1199169408 1159853696 81113368 1233995136 949062208 837867968 957249152 297462976 1155556480 1133087232 969292800 1254002432 731637056 867644800 1083843584 1037053696 Palmitoleic acid* 3110272 2249411 319965888 413373632 387915104 382503904 16476682 18265176 24553832 32329226 12192351 12924673 13038534 16750778 262454528 266116240 269540320 280003648 273695008 273124416 297126528 289236704 293320064 306060992 311676192 470167424 309080704 267703792 243541344 257364880 552374720 616190592 520357728 240434080 216714416 91175840 137352400 188384928 195108096 228174160 231688960 531193568 490257056 531574048 401213728 208494752 330423744 12979286 334852032 454125888 581272448 453655488 62067472 593745216 428566112 482312576 481577952 284707968 368832672 477888288 399666944 Pentadecanoic acid* 3562624 3558820 27755426 26305824 26579948 27430368 29399034 35330448 36384964 59396720 31694044 35154700 40117956 37906420 49107632 48376060 49487988 50866384 50571256 50504708 53341300 54262584 54797140 57762476 55763196 54603972 47089432 30436014 41083552 52674232 136298848 137430112 139786240 148286304 78748288 71169976 100219664 48358360 36202948 30527534 38149052 28154372 34955724 31996082 40704016 44927156 33135084 7592817 39616104 45753604 36733572 47410744 26220482 50216352 60505552 54421820 37961680 39406564 39043488 35918272 48558804 Pentose 5-phosphate 3089 0 497372 625002 481855 671704 575650 418349 1160695 821717 842298 1015182 1047386 1127418 867028 925098 908215 944935 885560 933873 922293 986858 944671 933678 932736 666480 765536 625228 556017 506364 272183 15874 43158 475795 1286938 739035 1277404 1744473 1252792 1652249 1323217 722415 649545 659584 663993 449971 526056 693136 901767 1328136 1032179 1276338 1670844 787701 895783 921834 1195313 1089092 680692 706153 738908 Phenylpyruvic acid 3710 11314 2588058 3920666 3902758 3798998 5164580 4615374 4168050 5199254 2806454 2630466 2081683 2419038 2874666 3208346 3607422 3329195 3522264 3419212 3731496 3595418 3917372 4356318 4187568 2481954 2983092 2598200 2504384 4419336 4134082 2329168 3184891 2133998 2961937 3710374 3134326 3246506 3209716 3625539 3149546 2737156 2764112 2390018 2902719 2051249 2184792 2091503 2558171 3734510 4484678 4505996 3278217 3242888 3775392 3632622 3347733 3300831 3544861 2798250 4172936 Phosphoenolpyruvic acid 384312 408297 3262997 2577667 3809355 3658217 6479458 4368690 6578023 9856404 3384039 3329421 2759324 3419578 2798836 3345796 3400245 3258281 3237622 3411373 2968160 3064577 3047521 2974766 3013448 2199601 2931164 2141358 3128243 2712027 1399160 455023 531414 778387 1882449 2333879 2401726 5493870 3704610 5722647 14432652 2244614 2345740 2117794 2774428 1360152 1252755 1402628 2470029 4357066 5538082 7356917 4028890 2422071 2900732 2999663 3727625 2692974 1865454 2045816 2240834 Phosphoserine 2940 0 163378 270833 186517 232513 50747 45436 63229 81906 53770 61957 62823 93731 114989 112157 132971 129405 124810 120387 120984 115713 131286 132504 136627 122562 194489 128441 147466 61734 37929 2085 1110 17842 262752 83780 90670 235287 191483 226004 137073 64611 124477 79744 92014 128224 53512 501707 166254 78171 75349 103008 166573 100358 105729 147743 175919 85706 83948 122247 98392 Pyroglutamic acid 136947 66844 46400968 42959512 32681146 39712660 9279727 8727951 8171842 8896819 6647598 7928928 8384190 11340346 20715108 22070052 23584174 21541742 25475348 24123676 23539368 22401474 23121466 24124918 23114634 20477384 33302412 22183516 29598648 12963682 5564391 617194 102044 3934752 51245052 14621454 15808763 45371324 32453772 38607236 30105560 12977256 19507028 18107774 18226484 22151322 11905756 43937648 34080528 15658821 17380774 21762890 17447564 19947746 22514480 32121714 32161528 22008432 28839196 29047662 26584762 Pyruvic acid 87629 260277 2030399 2547529 1887187 2488820 1027272 862122 4005672 3385889 1006159 651941 571664 792106 2930327 3224344 3355396 3393090 3365263 3304869 3221774 3233942 3162724 2981924 2996263 4807201 3363228 2620611 2359490 1730440 1314663 584804 961457 1188517 2479028 897800 1367826 11342443 8919394 9380442 2991796 3617594 4317242 4928784 3740256 5427362 4928768 24329024 9180461 993138 1483932 1657106 5033846 2582971 3498650 2827817 3095102 1325490 1758784 2564689 1699215 Ribose 5-phosphate 2947 5813 530814 500122 517945 484983 397588 314970 316556 332175 276834 376486 331586 410725 467324 501307 512518 520548 545912 537620 489734 503756 524155 546594 571244 489040 549651 624811 634497 124908 87373 3569 5698 40876 205590 218423 256295 708045 590894 754838 888429 727554 694112 542231 639974 372759 465526 294691 843050 696938 724534 642808 539369 631387 647054 701637 835157 682318 383779 485893 465370 Ribose* 17357 137130 159791 55667 54325 48744 75257 81865 101873 94489 162701 151536 159143 219065 165048 175511 211615 155161 177652 186736 178305 157776 190051 175490 180475 119745 57972 55030 106420 43876 119452 24394 34706 90072 215830 149273 165852 171413 105813 176716 214258 215277 165597 153073 197422 99301 198505 122796 274115 299527 220663 273710 230811 250407 204967 239827 331476 388604 474644 504558 447568 Serine 4642 4829 215045 207026 189770 215402 78265 93090 104675 95858 86599 109398 102028 132713 208460 192058 217400 199272 224341 209123 201199 225653 217559 233986 207650 404420 332342 253782 186342 87489 78485 11133 19306 72591 193860 130823 107421 245130 188025 235744 533501 172677 196621 172901 175097 206073 135423 272579 286957 162325 164094 150671 182400 180748 183222 203006 260567 215067 220697 221905 207091 Sorbitol-6-phosphate* 5940 5375 4656204 6034028 6501206 5778278 1592858 720420 1030486 1620982 1550891 1659364 1407553 1948610 4400156 4819520 4614535 5181143 4965962 4847942 4785066 4780956 4726992 4780465 4822132 4867999 6249396 5843353 5407174 270380 98754 11314 17206 102094 375882 108857 530157 7852328 6662858 8147326 5745308 5983934 5974403 7209545 6344280 3139424 5598351 3128818 5858834 8228488 8322240 7896442 8150606 10987855 8297542 9544520 9554219 7260867 6964916 13414460 7932770 Stearic acid* 43658556 37154560 502424672 618351552 543754560 496714816 555765888 545614400 858288640 875617408 857010048 493126528 507578080 569469824 715101504 697716672 726731392 731636864 743534144 716338752 749672704 737931072 751616896 755353664 747009600 1267200384 991153088 585317312 942114560 752770240 1888585984 1557413376 1198800000 1596176000 621138944 921201152 1338841088 473249760 533059136 435383808 724581440 549934080 594950784 598239360 447932736 834395072 674356352 44215180 714537792 595525120 488003488 595010496 255963312 681498624 769077376 658048576 719504448 442523104 492819648 582995456 678802816 Succinic acid 2736828 2050638 291278752 319353152 394860864 401389024 390891776 316776480 329169472 320960032 268926880 281490304 288227680 315849120 258189776 264205824 268706400 266487728 263666000 259202976 246242800 256851456 256823776 253931248 254767008 277897376 328930240 244359728 296673856 207936384 245213920 174335120 233429568 209435776 222858112 249361952 250102384 346148640 262350016 347954496 379337440 166135200 182331664 196601920 224701024 132607536 155551872 167962960 209266416 311073728 366088352 357894176 357605664 257340128 230835872 247755408 245873392 215899584 162186448 153701872 189622288 Threonine 27341 46293 201160 215632 227725 241150 37048 43811 48611 63777 49691 41927 22610 46598 797034 943848 842784 866938 853997 819912 890534 856461 807405 873342 944505 521913 451341 461659 490040 55971 30497 33301 17631 18717 34413 32971 30954 795479 521072 568910 464978 2576750 2746441 4530066 4063270 3845980 5075074 6249204 9835415 278341 259784 287291 457133 596598 951921 875449 901728 1063632 1532574 2693072 1435214 Thymidine* 142343 431025 1882739 792601 565322 919724 1056260 813600 4060194 3563189 1854511 1419068 1149931 1334148 1001899 962752 1166415 986245 1138812 1165472 1017587 939666 1184284 1091400 886009 553526 610110 295042 546030 950999 908193 571407 1492872 2282187 2859015 1558220 2234773 949242 536257 589997 335646 733483 982525 1005400 760149 1094756 1283019 1396470 1446336 186769 202739 228763 1097066 320098 243553 209550 622093 758715 696675 548768 603268 Thymine* 145398 483378 11075350 15083367 19074700 16806940 9200941 8119329 7631427 7868940 3227776 2514629 1728603 2027004 7416814 7121683 7743306 8387950 7774540 7372100 8391678 7776710 7862241 8781262 8553556 9728898 10024272 8565108 8011631 3031218 2164253 2333599 2583468 2089334 2380632 2120427 2315064 8345469 5704062 7917501 6440451 9103905 9100632 11337833 10134188 7588296 9660106 15690689 12498423 7907832 7019396 6896171 13738166 5287712 5151120 5904626 5063932 8369912 6659008 5123508 6998095 trans-Aconitic acid* 132340 243654 249978 189562 191793 201522 234467 250175 318556 292994 347202 318044 324216 295418 415567 384978 368933 397694 342008 376611 313838 298333 287411 270975 278703 219404 149459 171481 231912 153091 184761 171080 168101 219624 201986 199333 241317 330380 186656 193261 236846 540299 437870 625179 432159 1248978 1040563 1057145 1424206 199561 199188 240844 199778 435695 411716 391078 390961 717480 637260 434316 507442 Tridecanoic acid* 1600749 1331149 18186830 16025534 16468111 19112428 21700290 30401610 22962748 35300620 18714700 28046144 24157282 23234212 32747820 32006472 33338828 33748688 34340416 35670708 36445840 35020404 36683408 35045308 37943940 36550000 29701772 18903204 20378866 23732686 66014264 96197544 86022416 65598236 24378964 34629408 59621112 36615964 21710564 24900600 26429336 16377760 29062318 20444524 27476824 30720836 22709946 10193745 41496452 40690456 27158338 42135104 19362202 31858502 45743000 38981572 22087992 27411290 29646826 26449378 37404552 Uracil* 214436 111142 187407120 209312336 242589280 229331728 143681248 96155888 103063416 108884704 57118028 40917644 29162748 43860576 148705792 146666512 157601296 162609584 153207280 159689616 152925984 153603504 163876704 158671072 157385776 185782576 183302432 145451920 173213104 108355488 111294248 99837984 110667528 106257720 101237816 92325688 101358376 226815168 177922048 226413920 210793648 146550400 149062528 171501120 157244544 90385736 132876656 103952160 173781680 234353088 236070528 237379664 240388096 165281872 186074512 182427168 176720272 165329328 148708736 157334704 189808528 Ureidosuccinic acid 0 7992 222160 156749 414484 381683 38671 22801 12585 6578 4104 4457 3893 3137 53059 64910 49971 60070 63041 58921 53200 58757 46912 43726 37458 10082 15920 3088 5861 2740 4141 2579 3763 0 7779 5450 429 66400 34783 43946 168855 231276 213998 337849 203013 17575 10909 26025 27127 33716 34043 68785 55357 38953 64736 123083 95928 17890 20306 7498 12438 Uridine 3084 0 3403657 5028331 4302662 5756601 6188159 5745466 13598235 10497524 9668700 10119476 9300551 10574089 8242680 8787812 9274185 8954665 9409427 9435588 9178946 9543830 9733945 9477943 9560775 5624955 6966520 3904959 4647586 9304522 9390768 5192938 8664327 15514974 14484005 11224105 14304587 15980677 11400489 14412035 11266842 5470826 5355600 6554396 6245192 4310122 6186250 5380842 9815204 8496860 8105806 9759050 13872979 7908657 8847269 8567194 10917906 10519268 8344448 10261570 8846061 Uridine 5-diphosphate 33937 48210 2592428 2717553 3202098 2516278 3199418 2912792 2642570 2360960 2008237 2399035 2251404 2444130 3238478 3393124 3522870 3493631 3454088 3465529 3314272 3414338 3428027 3381806 3391640 3097446 3962408 3488342 3874682 1682370 762938 16446 49770 376146 1796507 2694231 1744546 6702460 5273459 6545672 8676518 3619361 3923181 3479272 3516044 2730874 2727344 2998379 4457329 5247724 6522650 7283156 4899106 3222578 4029300 3818183 4775364 3534925 2231554 1850910 2975090 Uridine diphosphate glucose 0 0 43216840 69810552 62627388 84466720 83208888 48037160 84389328 36610488 18135146 30016008 12145165 25257854 36572540 64960768 37823968 65999372 68033384 66163988 33905944 33533458 37947052 33740616 36763572 38365976 52094512 32909668 34382740 12609963 4127381 281686 531729 4332761 19694094 37816448 42131412 40640780 23945282 37408504 52383120 34930740 32766980 64437664 61748000 33852932 38839020 19243926 71044368 39877520 39513724 60537536 52984512 53351308 44231004 74086576 53936940 58877100 27833392 16084614 28475074 Xanthine 25694 6225 58661056 66850680 79370736 73048168 62886540 48214128 49081760 47912404 35731880 35193496 31241636 37250016 45525712 49781200 51258108 51703768 52817568 52318488 51136748 51905428 52288344 53139724 50624128 57881492 53784872 43189080 49335156 59013412 72939672 79124544 89798040 72188640 49125632 54456264 60034168 56154412 46190224 57144540 60336388 40732244 40224184 47071324 49713784 25204018 30430858 23403300 39760364 64441628 73943336 78147416 62284328 53053816 53373516 54955512 52302324 42418312 35491940 40632084 43704512 Xanthosine monophosphate* 0 0 126998 160624 147963 148802 72995 60760 117103 131790 495929 843675 1538880 1494122 131890 171639 191992 186647 169241 175896 203888 184971 181139 184913 173771 163439 184708 141383 135157 182226 119349 0 0 48613 126580 124859 175457 217004 181973 211111 220216 72015 76670 100570 90535 55310 85133 67356 92461 186764 221999 244403 222530 114755 125328 94418 79752 109849 71100 49249 96109 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Average Rt(min) Average Mz Adduct type Formula INCHIKEY SMILES Deoxyuridine* 1.875 263.04404 [M+Cl]- C9H12N2O5 MXHRCPNRJAMMIM-SHYZEUOFBK c1cn(c(=O)[nH]c1=O)[C@H]2C[C@@H]([C@H](O2)CO)O Sorbitol 7.575 217.04871 [M+Cl]- C6H14O6 FBPFZTCFMRRESA-JGWLITMVSA-N OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO 2,3-Dihydroxybenzoic acid 3.269 153.01958 [M-H]- C7H6O4 GLDQAMYCGOIJDV-UHFFFAOYAE c1cc(c(c(c1)O)O)C(=O)O 2-Aminobutanoic acid 8.032 102.05617 [M-H]- C4H9NO2 QWCKQJZIFLGMSD-UHFFFAOYAG CCC(C(=O)O)N 2-Hydroxyisocaproic acid 2.364 131.07147 [M-H]- C6H12O3 LVRFTAZAXQPQHI-UHFFFAOYSA-N CC(C)CC(O)C(O)=O 2-Ketobutyric acid 2.91 101.02446 [M-H]- C4H6O3 TYEYBOSBBBHJIV-UHFFFAOYAT CCC(=O)C(=O)O 2-Phosphoglyceric acid 13.716 184.98595 [M-H]- C3H7O7P GXIURPTVHJPJLF-UHFFFAOYAQ C(C(C(=O)O)OP(=O)(O)O)O 3-Phenyllactic acid 2.277 165.05597 [M-H]- C9H10O3 VOXXWSYKYCBWHO-UHFFFAOYAI c1ccc(cc1)CC(C(=O)O)O 3-Phosphoglyceric Acid 14.189 184.98593 [M-H]- C3H7O7P OSJPPGNTCRNQQC-UHFFFAOYSA-N OC(COP(O)(O)=O)C(O)=O 3-Uridine Monophosphate 11.734 323.02869 [M-H]- C9H13N2O9P FOGRQMPFHUHIGU-XVFCMESIBZ c1cn(c(=O)[nH]c1=O)[C@H]2[C@@H]([C@@H]([C@H](O2)CO)OP(=O)(O)O)O Adenosine Cyclophosphate 6.53 328.04523 [M-H]- C10H12N5O6P IVOMOUWHDPKRLL-HMEJCUHCBO c1nc(c2c(n1)n(cn2)[C@H]3C([C@@H]4[C@H](O3)COP(=O)(O4)O)O)N Adenosine diphosphate 12.714 426.02271 [M-H]- C10H15N5O10P2 XTWYTFMLZFPYCI-KQYNXXCUBP c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)O)O)O)N Adenosine diphosphate ribose* 12.088 558.06555 [M-H]- C15H23N5O14P2 SRNWOUGRCWSEMX-MRUDJCSFSA-N NC1=NC=NC2=C1N=CN2C1O[C@H](COP(O)(=O)OP(O)(=O)OC[C@H]2O[C@@H](O)[C@H](O)[C@@H]2O)[C@@H](O)[C@H]1O Adenosine monophosphate 11.274 346.05615 [M-H]- C10H14N5O7P UDMBCSSLTHHNCD-KQYNXXCUBP c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)O)O)O)N Adenosine triphosphate 14.003 505.98865 [M-H]- C10H16N5O13P3 ZKHQWZAMYRWXGA-KQYNXXCUBG c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O)N alpha-Ketoisovaleric acid 2.257 115.04023 [M-H]- C5H8O3 QHKABHOOEWYVLI-UHFFFAOYAY CC(C)C(=O)C(=O)O Aminoadipic acid 11.871 160.06161 [M-H]- C6H11NO4 OYIFNHCXNCRBQI-BYPYZUCNBJ C(C[C@@H](C(=O)O)N)CC(=O)O Aspartic acid 11.608 132.03061 [M-H]- C4H7NO4 CKLJMWTZIZZHCS-REOHCLBHBW C([C@@H](C(=O)O)N)C(=O)O Azelaic acid 9.647 187.09776 [M-H]- C9H16O4 BDJRBEYXGGNYIS-UHFFFAOYAK C(CCCC(=O)O)CCCC(=O)O cis-Aconitic acid 13.096 173.00949 [M-H]- C6H6O6 GTZCVFVGUGFEME-IWQZZHSRBS C(/C(=C/C(=O)O)/C(=O)O)C(=O)O Citraconic acid 2.959 129.01959 [M-H]- C5H6O4 HNEGQIOMVPPMNR-IHWYPQMZBK C/C(=C/C(=O)O)/C(=O)O Citramalic acid* 12.421 147.02997 [M-H]- C5H8O5 XFTRTWQBIOMVPK-UHFFFAOYAT CC(CC(=O)O)(C(=O)O)O Cytidine 5.472 242.07851 [M-H]- C9H13N3O5 UHDGCWIWMRVCDJ-XVFCMESIBD c1cn(c(=O)nc1N)[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O Cytidine 5-monophosphate 12.788 322.04486 [M-H]- C9H14N3O8P IERHLVCPSMICTF-XVFCMESIBY c1cn(c(=O)nc1N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)O)O)O Cytidine diphosphate 14.12 402.01175 [M-H]- C9H15N3O11P2 ZWIADYZPOWUWEW-XVFCMESIBF c1cn(c(nc1=N)O)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)OP(=O)(O)O)O)O Cytidine triphosphate 15.613 481.97696 [M-H]- C9H16N3O14P3 PCDQPRRSZKQHHS-XVFCMESIBL c1cn(c(=O)nc1N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O Deoxyadenosine monophosphate 10.801 330.06168 [M-H]- C10H14N5O6P KHWCHTKSEGGWEX-RRKCRQDMBS c1nc(c2c(n1)n(cn2)[C@H]3C[C@@H]([C@H](O3)COP(=O)(O)O)O)N Deoxyguanosine monophosphate* 12.448 346.05606 [M-H]- C10H14N5O7P LTFMZDNNPPEQNG-KVQBGUIXBV c1nc2c(n1[C@H]3C[C@@H]([C@H](O3)COP(=O)(O)O)O)[nH]c(=N)nc2O Deoxyinosine 2.973 251.07857 [M-H]- C10H12N4O4 VGONTNSXDCQUGY-RRKCRQDMBC c1nc2c(c(n1)O)ncn2[C@H]3C[C@@H]([C@H](O3)CO)O Dihydroorotic acid* 7.335 157.02554 [M-H]- C5H6N2O4 UFIVEPVSAGBUSI-UHFFFAOYAI C1C(NC(=O)NC1=O)C(=O)O Fructose-1,6-diphosphate 16.223 338.98904 [M-H]- C6H14O12P2 RNBGYGVWRKECFJ-ARQDHWQXBZ C([C@@H]1[C@H]([C@@H]([C@](O1)(COP(=O)(O)O)O)O)O)OP(=O)(O)O Fructose-6-phosphate 12.759 259.02249 [M-H]- C6H13O9P BGWGXPAPYGQALX-ARQDHWQXSA-N OC[C@@]1(O)O[C@H](COP(O)(O)=O)[C@@H](O)[C@@H]1O Fumaric acid 12.966 115.00386 [M-H]- C4H4O4 VZCYOOQTPOCHFL-OWOJBTEDBF C(=C/C(=O)O)C(=O)O Glucosamine 6-phosphate 13.649 258.03854 [M-H]- C6H14NO8P XHMJOUIAFHJHBW-VFUOTHLCBT C([C@@H]1[C@@H]([C@@H]([C@H]([C@@H](O1)O)N)O)O)OP(=O)(O)O Glucose-6-phosphate 13.463 259.02246 [M-H]- C6H13O9P NBSCHQHZLSJFNQ-GASJEMHNSA-N OC(O1)[C@H](O)[C@@H](O)[C@H](O)[C@H]1COP(O)(O)=O Glutamic acid 11.508 146.04602 [M-H]- C5H9NO4 WHUUTDBJXJRKMK-VKHMYHEABN C(CC(=O)O)[C@@H](C(=O)O)N Glutathione (GSH) 11.267 306.07666 [M-H]- C10H17N3O6S Glutathione (GSSG) 14.567 611.14551 [M-H]- C20H32N6O12S2 YPZRWBKMTBYPTK-BJDJZHNGBD C(CC(=O)N[C@@H](CSSC[C@@H](C(=O)NCC(=O)O)NC(=O)CC[C@@H](C(=O)O)N)C(=O)NCC(=O)O)[C@@H](C(=O)O)N Glyceric acid* 7.89 105.01947 [M-H]- C3H6O4 RBNPOMFGQQGHHO-UHFFFAOYSA-N C(C(C(=O)O)O)O Glycerol 3-Phosphate 12.01 171.00641 [M-H]- C3H9O6P AWUCVROLDVIAJX-GSVOUGTGSA-N OC[C@@H](O)COP(O)(O)=O Glycine 9.8 74.02487 [M-H]- C2H5NO2 DHMQDGOQFOQNFH-UHFFFAOYAW C(C(=O)O)N Guanosine diphosphate 14.607 442.01788 [M-H]- C10H15N5O11P2 QGWNDRXFNXRZMB-UUOKFMHZBH c1nc2c(=O)[nH]c(nc2n1[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)O)O)O)N Guanosine monophosphate 13.098 362.05173 [M-H]- C10H14N5O8P RQFCJASXJCIDSX-UUOKFMHZBU c1nc2c(=O)[nH]c(nc2n1[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)O)O)O)N Heptadecanoic acid* 0.767 269.24887 [M-H]- C17H34O2 KEMQGTRYUADPNZ-UHFFFAOYSA-N CCCCCCCCCCCCCCCCC(=O)O Heptanoic acid* 1.494 129.09216 [M-H]- C7H14O2 MNWFXJYAOYHMED-UHFFFAOYSA-N CCCCCCC(O)=O Hexose-phosphate 12.953 259.02249 [M-H]- C6H13O9P RHKKZBWRNHGJEZ-ARQDHWQXSA-N OC[C@H]1O[C@](O)(COP(O)(O)=O)[C@@H](O)[C@@H]1O Indole-3-Pyruvic Acid* 2.857 202.05124 [M-H]- C11H9NO3 RSTKLPZEZYGQPY-UHFFFAOYAO c1ccc2c(c1)c(c[nH]2)CC(=O)C(=O)O Inosine 4.384 267.07336 [M-H]- C10H12N4O5 UGQMRVRMYYASKQ-KQYNXXCUBA c1nc2c(c(n1)O)ncn2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)O)O Inosine monophosphate 12.092 347.0405 [M-H]- C10H13N4O8P GRSZFWQUAKGDAV-KQYNXXCUBU c1[nH]c2c(c(=O)n1)ncn2[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)O)O)O Ketoglutaric acid 12.094 145.01454 [M-H]- C5H6O5 KPGXRSRHYNQIFN-UHFFFAOYAN C(CC(=O)O)C(=O)C(=O)O Lactic Acid* 6.347 89.02454 [M-H]- C3H6O3 JVTAAEKCZFNVCJ-REOHCLBHSA-N C[C@H](O)C(O)=O Linolenic acid* 0.784 277.21732 [M-H]- C18H30O2 DTOSIQBPPRVQHS-UHFFFAOYSA-N CCC=CCC=CCC=CCCCCCCCC(=O)O LPC 14:1* 1.611 464.2789 [M-H]- null null null Maleic acid 2.844 115.00381 [M-H]- C4H4O4 VZCYOOQTPOCHFL-UPHRSURJBG C(=CC(=O)O)C(=O)O Methylmalonic acid 7.883 117.01942 [M-H]- C4H6O4 ZIYVHBGGAOATLY-UHFFFAOYAT CC(C(=O)O)C(=O)O Myoinositol 9.905 179.05618 [M-H]- C6H12O6 CDAISMWEOUEBRE-GPIVLXJGSA-N O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O N-Acetyl-aspartic acid 11.881 174.04099 [M-H]- C6H9NO5 OTCCIMWXFLJLIA-BYPYZUCNBM CC(=O)N[C@@H](CC(=O)O)C(=O)O N-Acetylglutamine* 7.979 187.07274 [M-H]- C7H12N2O4 KSMRODHGGIIXDV-YFKPBYRVBV OC(=O)CCCCCC(O)=O N-Acetyl-leucine 2.992 172.09821 [M-H]- C8H15NO3 WXNXCEHXYPACJF-UHFFFAOYAT CC(C)CC(C(=O)O)NC(=O)C N-Acetylmethionine 3.474 190.05464 [M-H]- C7H13NO3S XUYPXLNMDZIRQH-UHFFFAOYAQ CC(=O)NC(CCSC)C(=O)O N-Acetylneuraminic acid* 10.323 308.09921 [M-H]- C11H19NO9 SQVRNKJHWKZAKO-PFQGKNLYBI CC(=O)N[C@@H]1[C@H](C[C@](O[C@H]1[C@@H]([C@@H](CO)O)O)(C(=O)O)O)O NADH* 11.314 664.11688 [M-H]- C21H29N7O14P2 BOPGDPNILDQYTO-NNYOXOHSBA c1nc(c2c(n1)n(cn2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)OC[C@@H]4[C@H]([C@H]([C@@H](O4)N5C=CCC(=C5)C(=O)N)O)O)O)O)N NADP 14.3 742.06946 [M-H]- C21H28N7O17P3 XJLXINKUBYWONI-NNYOXOHSBN c1cc(c[n+](c1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)(O)OC[C@@H]3[C@H]([C@H]([C@@H](O3)n4cnc5c4ncnc5N)OP(=O)(O)O)O)O)O)C(=O)N Oleic acid* 0.765 281.24841 [M-H]- C18H34O2 ZQPPMHVWECSIRJ-KTKRTIGZSA-N CCCCCCCC/C=CCCCCCCCC(=O)O Orotic acid 5.663 155.00995 [M-H]- C5H4N2O4 PXQPEWDEAKTCGB-UHFFFAOYAD c1c([nH]c(=O)[nH]c1=O)C(=O)O Ortophosphate* 12.511 96.96962 [M-H]- H3O4P NBIIXXVUZAFLBC-UHFFFAOYSA-N OP(=O)(O)O Palmitic acid* 0.779 255.23318 [M-H]- C16H32O2 IPCSVZSSVZVIGE-UHFFFAOYSA-N OC(=O)CCCCCCCCCCCCCCC Palmitoleic acid* 0.788 253.21725 [M-H]- C16H30O2 SECPZKHBENQXJG-FPLPWBNLSA-N CCCCCC/C=CCCCCCCCC(=O)O Pentadecanoic acid* 0.782 241.21764 [M-H]- C15H30O2 WQEPLUUGTLDZJY-UHFFFAOYSA-N CCCCCCCCCCCCCCC(=O)O Pentose 5-phosphate 12.056 229.01175 [M-H]- C5H11O8P KTVPXOYAKDPRHY-AIHAYLRMBX C([C@@H]1[C@H]([C@H]([C@H](O1)O)O)O)OP(=O)(O)O Phenylpyruvic acid 1.684 163.0403 [M-H]- C9H8O3 BTNMPGBKDVTSJY-UHFFFAOYAU c1ccc(cc1)CC(=O)C(=O)O Phosphoenolpyruvic acid 14.594 166.97525 [M-H]- C3H5O6P DTBNBXWJWCWCIK-UHFFFAOYSA-N OC(=O)C(=C)OP(O)(O)=O Phosphoserine 13.431 184.00208 [M-H]- C3H8NO6P BZQFBWGGLXLEPQ-REOHCLBHSA-N N[C@@H](COP(O)(O)=O)C(O)=O Pyroglutamic acid 7.557 128.0354 [M-H]- C5H7NO3 ODHCTXKNWHHXJC-VKHMYHEABY C1CC(=O)N[C@@H]1C(=O)O Pyruvic acid 4.145 87.0088 [M-H]- C3H4O3 LCTONWCANYUPML-UHFFFAOYAJ CC(=O)C(=O)O Ribose 5-phosphate 12.499 229.01199 [M-H]- C5H11O8P KTVPXOYAKDPRHY-AIHAYLRMBX C([C@@H]1[C@H]([C@H]([C@H](O1)O)O)O)OP(=O)(O)O Ribose* 3.924 149.04581 [M-H]- C5H10O5 HMFHBZSHGGEWLO-SOOFDHNKBV C([C@@H]1[C@H]([C@H](C(O1)O)O)O)O Serine 10.168 104.03551 [M-H]- C3H7NO3 MTCFGRXMJLQNBG-REOHCLBHBX C([C@@H](C(=O)O)N)O Sorbitol-6-phosphate* 12.866 261.03839 [M-H]- C6H15O9P GACTWZZMVMUKNG-SLPGGIOYSA-N O=P(O)(O)OCC(O)C(O)C(O)C(O)CO Stearic acid* 0.759 283.26462 [M-H]- C18H36O2 QIQXTHQIDYTFRH-UHFFFAOYSA-N CCCCCCCCCCCCCCCCCC(=O)O Succinic acid 12 117.01943 [M-H]- C4H6O4 KDYFGRWQOYBRFD-UHFFFAOYAC C(CC(=O)O)C(=O)O Threonine 9.175 118.05106 [M-H]- C4H9NO3 AYFVYJQAPQTCCC-GBXIJSLDBG C[C@H]([C@@H](C(=O)O)N)O Thymidine* 1.612 241.08282 [M-H]- C10H14N2O5 IQFYYKKMVGJFEH-XLPZGREQBQ Cc1cn(c(=O)[nH]c1=O)[C@H]2C[C@@H]([C@H](O2)CO)O Thymine* 1.596 125.03576 [M-H]- C5H6N2O2 RWQNBRDOKXIBIV-UHFFFAOYAL Cc1c[nH]c(=O)[nH]c1=O trans-Aconitic acid* 15.176 173.00917 [M-H]- C6H6O6 GTZCVFVGUGFEME-HNQUOIGGBQ C(/C(=CC(=O)O)/C(=O)O)C(=O)O Tridecanoic acid* 0.815 213.18614 [M-H]- C13H26O2 SZHOJFHSIKHZHA-UHFFFAOYSA-N CCCCCCCCCCCCC(=O)O Uracil* 1.711 111.02005 [M-H]- C4H4N2O2 ISAKRJDGNUQOIC-UHFFFAOYSA-N OC1=NC=CC(O)=N1 Ureidosuccinic acid 13.245 175.03638 [M-H]- C5H8N2O5 HLKXYZVTANABHZ-UHFFFAOYAH C(C(C(=O)O)NC(=O)N)C(=O)O Uridine 2.767 243.06262 [M-H]- C9H12N2O6 DRTQHJPVMGBUCF-XVFCMESIBG c1cn(c(=O)[nH]c1=O)[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O Uridine 5-diphosphate 13.229 402.99536 [M-H]- C9H14N2O12P2 XCCTYIAWTASOJW-XVFCMESIBB c1cn(c(=O)nc1O)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)OP(=O)(O)O)O)O Uridine diphosphate glucose 12.71 565.0481 [M-H]- C15H24N2O17P2 HSCJRCZFDFQWRP-JZMIEXBBBU c1cn(c(=O)[nH]c1=O)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)OP(=O)(O)O[C@@H]3[C@@H]([C@H]([C@@H]([C@H](O3)CO)O)O)O)O)O Xanthine 3.804 151.02626 [M-H]- C5H4N4O2 LRFVTYWOQMYALW-UHFFFAOYAT c1[nH]c2c(n1)[nH]c(=O)[nH]c2=O Xanthosine monophosphate* 14.734 363.03485 [M-H]- C10H13N4O9P DCTLYFZHFGENCW-UHFFFAOYSA-N C1=NC2=C(N1C3C(C(C(O3)COP(=O)(O)O)O)O)NC(=O)NC2=O METABOLITES_END #END