#METABOLOMICS WORKBENCH siokatgs_20250219_072847 DATATRACK_ID:5648 STUDY_ID:ST003743 ANALYSIS_ID:AN006147 PROJECT_ID:PR002329
VERSION                          	1
CREATED_ON                       	04-08-2025
#PROJECT
PR:PROJECT_TITLE                 	Hepato-cardiac interorgan communication controls cardiac hypertrophy via
PR:PROJECT_TITLE                 	combined endocrine-autocrine FGF21 signaling.
PR:PROJECT_SUMMARY               	Fibroblast Growth Factor (FGF) 21 is a hormone produced mainly by the liver but
PR:PROJECT_SUMMARY               	also other organs, including the heart. Although FGF21 analogs are used for
PR:PROJECT_SUMMARY               	treating obesity and metabolic syndrome in humans, preclinical and clinical
PR:PROJECT_SUMMARY               	studies have elicited mixed results about whether prolonged FGF21 signaling is
PR:PROJECT_SUMMARY               	protective or detrimental for cardiac function. Based on our findings, showing
PR:PROJECT_SUMMARY               	elevated serum and cardiac FGF21 levels in humans with increased left
PR:PROJECT_SUMMARY               	ventricular afterload, we explored involvement of FGF21 in cardiac hypertrophy.
PR:PROJECT_SUMMARY               	Our mouse studies revealed an interorgan liver-heart crosstalk mechanism which
PR:PROJECT_SUMMARY               	is controlled by initial hepatic FGF21 release followed by induction of
PR:PROJECT_SUMMARY               	cardiomyocyte FGF21 expression. Tissue-specific genetic ablation or anti-sense
PR:PROJECT_SUMMARY               	oligonucleotides-based inhibition of FGF21 showed that in response to pressure
PR:PROJECT_SUMMARY               	overload, cardiomyocyte FGF21 upregulation is a critical event that is
PR:PROJECT_SUMMARY               	stimulated by liver-derived FGF21 and drives cardiac hypertrophy likely by
PR:PROJECT_SUMMARY               	interfering with cardioprotective oxytocin signaling. Conclusively, a
PR:PROJECT_SUMMARY               	hepato-cardiac FGF21-based signaling axis governs cardiac hypertrophy.
PR:INSTITUTE                     	University of Cincinnati College of Medicine
PR:LAST_NAME                     	Siokatas
PR:FIRST_NAME                    	Georgios
PR:ADDRESS                       	231 ALBERT SABIN WAY, University of Cincinnati, Cincinnati, OH, 45267-2827
PR:EMAIL                         	siokatgs@ucmail.uc.edu
PR:PHONE                         	5133028282
PR:DOI                           	http://dx.doi.org/10.21228/M8KJ93
#STUDY
ST:STUDY_TITLE                   	Cardiac metabolome signatures of hepato-cardiac FGF21 signaling in pressure
ST:STUDY_TITLE                   	overload-induced cardiac hypertrophy
ST:STUDY_SUMMARY                 	This study investigates the early (3 days) mid- (2 weeks) and late (8 weeks)
ST:STUDY_SUMMARY                 	cardiac metabolome changes driven by FGF21 signaling in the context of pressure
ST:STUDY_SUMMARY                 	overload-induced cardiac hypertrophy. Targeted NMR-based metabolomic profiling
ST:STUDY_SUMMARY                 	was performed on cardiac tissue from control mice at 3 days, 2 weeks, and 8
ST:STUDY_SUMMARY                 	weeks following transverse aortic constriction (TAC), as well as from
ST:STUDY_SUMMARY                 	hepatocyte-specific (HEP-FGF21) and cardiomyocyte specific (CM-FGF21) Fgf21
ST:STUDY_SUMMARY                 	knockout mice at the 8-week post-TAC timepoint. Sham-operated wild type mice and
ST:STUDY_SUMMARY                 	mice expressing Cre in hepatocytes or cardiomyocytes served as controls for each
ST:STUDY_SUMMARY                 	timepoint. The early and mid-timepoints were selected to capture transient and
ST:STUDY_SUMMARY                 	potentially triggering metabolic adaptations that precede overt cardiac
ST:STUDY_SUMMARY                 	remodeling, while the late timepoint allowed assessment of established metabolic
ST:STUDY_SUMMARY                 	reprogramming in advanced hypertrophy. Inclusion of the knockout models at 8
ST:STUDY_SUMMARY                 	weeks enabled dissection of the respective contributions of endocrine
ST:STUDY_SUMMARY                 	(hepatocyte-derived) and autocrine (cardiomyocyte-derived) FGF21 signaling to
ST:STUDY_SUMMARY                 	the cardiac metabolome phenotype. The analysis revealed time-dependent
ST:STUDY_SUMMARY                 	alterations in the metabolic profile, with lower glucose and glucose derived
ST:STUDY_SUMMARY                 	metabolites in early timepoints and accumulation of Branched-Chain Amino Acids
ST:STUDY_SUMMARY                 	when pathological hypertrophy has fully developed (8 weeks). Ablation of Fgf21
ST:STUDY_SUMMARY                 	in either the hepatocytes or cardiomyocytes mitigated maladaptive metabolic
ST:STUDY_SUMMARY                 	remodeling at 8 weeks, highlighting distinct and cooperative roles of hepatic
ST:STUDY_SUMMARY                 	and cardiac FGF21 in the progression of hypertrophy.
ST:INSTITUTE                     	University of Cincinnati College of Medicine
ST:LAST_NAME                     	Siokatas
ST:FIRST_NAME                    	Georgios
ST:ADDRESS                       	231 ALBERT SABIN WAY, University of Cincinnati, Cincinnati, OH, 45267-2827
ST:EMAIL                         	siokatgs@ucmail.uc.edu
ST:PHONE                         	5133028282
ST:SUBMIT_DATE                   	2025-02-19
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data
SUBJECT_SAMPLE_FACTORS           	6534	FGF-H36	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.011; RAW_FILE_NAME(raw file name)=FGF-H36
SUBJECT_SAMPLE_FACTORS           	6537	FGF-H37	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.022; RAW_FILE_NAME(raw file name)=FGF-H37
SUBJECT_SAMPLE_FACTORS           	6538	FGF-H38	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.025; RAW_FILE_NAME(raw file name)=FGF-H38
SUBJECT_SAMPLE_FACTORS           	6282	FGF-H39	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.012; RAW_FILE_NAME(raw file name)=FGF-H39
SUBJECT_SAMPLE_FACTORS           	7221	FGF-H40	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.026; RAW_FILE_NAME(raw file name)=FGF-H40
SUBJECT_SAMPLE_FACTORS           	H-5469	FGF-H11	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=Sham; weight (g)=0.022; RAW_FILE_NAME(raw file name)=FGF-H11
SUBJECT_SAMPLE_FACTORS           	H-5470	FGF-H12	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=Sham; weight (g)=0.038; RAW_FILE_NAME(raw file name)=FGF-H12
SUBJECT_SAMPLE_FACTORS           	H-5471	FGF-H13	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=Sham; weight (g)=0.035; RAW_FILE_NAME(raw file name)=FGF-H13
SUBJECT_SAMPLE_FACTORS           	H-3919	FGF-H14	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=Sham; weight (g)=0.024; RAW_FILE_NAME(raw file name)=FGF-H14
SUBJECT_SAMPLE_FACTORS           	H-3951	FGF-H15	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=Sham; weight (g)=0.036; RAW_FILE_NAME(raw file name)=FGF-H15
SUBJECT_SAMPLE_FACTORS           	H-6888	FGF-H16	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=TAC; weight (g)=0.054; RAW_FILE_NAME(raw file name)=FGF-H16
SUBJECT_SAMPLE_FACTORS           	H-6889	FGF-H17	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=TAC; weight (g)=0.045; RAW_FILE_NAME(raw file name)=FGF-H17
SUBJECT_SAMPLE_FACTORS           	H-6890	FGF-H18	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=TAC; weight (g)=0.061; RAW_FILE_NAME(raw file name)=FGF-H18
SUBJECT_SAMPLE_FACTORS           	H-6905	FGF-H19	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=TAC; weight (g)=0.037; RAW_FILE_NAME(raw file name)=FGF-H19
SUBJECT_SAMPLE_FACTORS           	H-6891	FGF-H20	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Surgery=TAC; weight (g)=0.043; RAW_FILE_NAME(raw file name)=FGF-H20
SUBJECT_SAMPLE_FACTORS           	H-5466	FGF-H01	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Surgery=Sham; weight (g)=0.026; RAW_FILE_NAME(raw file name)=FGF-H01
SUBJECT_SAMPLE_FACTORS           	H-5467	FGF-H02	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Surgery=Sham; weight (g)=0.029; RAW_FILE_NAME(raw file name)=FGF-H02
SUBJECT_SAMPLE_FACTORS           	H-3881	FGF-H04	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Surgery=Sham; weight (g)=0.033; RAW_FILE_NAME(raw file name)=FGF-H04
SUBJECT_SAMPLE_FACTORS           	H-3916	FGF-H05	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Surgery=Sham; weight (g)=0.021; RAW_FILE_NAME(raw file name)=FGF-H05
SUBJECT_SAMPLE_FACTORS           	H-8	FGF-H06	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Surgery=TAC; weight (g)=0.021; RAW_FILE_NAME(raw file name)=FGF-H06
SUBJECT_SAMPLE_FACTORS           	H-9	FGF-H07	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Surgery=TAC; weight (g)=0.035; RAW_FILE_NAME(raw file name)=FGF-H07
SUBJECT_SAMPLE_FACTORS           	H-10	FGF-H08	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Surgery=TAC; weight (g)=0.041; RAW_FILE_NAME(raw file name)=FGF-H08
SUBJECT_SAMPLE_FACTORS           	H-3	FGF-H09	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Surgery=TAC; weight (g)=0.028; RAW_FILE_NAME(raw file name)=FGF-H09
SUBJECT_SAMPLE_FACTORS           	H-6	FGF-H10	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Surgery=TAC; weight (g)=0.021; RAW_FILE_NAME(raw file name)=FGF-H10
SUBJECT_SAMPLE_FACTORS           	7367	FGF-H21	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=Sham; weight (g)=0.022; RAW_FILE_NAME(raw file name)=FGF-H21
SUBJECT_SAMPLE_FACTORS           	7313	FGF-H22	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=Sham; weight (g)=0.022; RAW_FILE_NAME(raw file name)=FGF-H22
SUBJECT_SAMPLE_FACTORS           	7370	FGF-H23	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=Sham; weight (g)=0.012; RAW_FILE_NAME(raw file name)=FGF-H23
SUBJECT_SAMPLE_FACTORS           	7368	FGF-H24	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=Sham; weight (g)=0.023; RAW_FILE_NAME(raw file name)=FGF-H24
SUBJECT_SAMPLE_FACTORS           	753	FGF-H25	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=Sham; weight (g)=0.022; RAW_FILE_NAME(raw file name)=FGF-H25
SUBJECT_SAMPLE_FACTORS           	7680	FGF-H26	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.028; RAW_FILE_NAME(raw file name)=FGF-H26
SUBJECT_SAMPLE_FACTORS           	7682	FGF-H27	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.035; RAW_FILE_NAME(raw file name)=FGF-H27
SUBJECT_SAMPLE_FACTORS           	7828	FGF-H28	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.029; RAW_FILE_NAME(raw file name)=FGF-H28
SUBJECT_SAMPLE_FACTORS           	7829	FGF-H29	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.031; RAW_FILE_NAME(raw file name)=FGF-H29
SUBJECT_SAMPLE_FACTORS           	7161	FGF-H30	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.024; RAW_FILE_NAME(raw file name)=FGF-H30
SUBJECT_SAMPLE_FACTORS           	H-5468	FGF-H03	Sample source:Heart | Genotype:- | Timepoint:Fgf21 fl/fl	Surgery=3 Days; weight (g)=Sham; RAW_FILE_NAME(raw file name)=0.02
SUBJECT_SAMPLE_FACTORS           	6357	FGF-H31	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.026; RAW_FILE_NAME(raw file name)=FGF-H31
SUBJECT_SAMPLE_FACTORS           	5814	FGF-H32	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.034; RAW_FILE_NAME(raw file name)=FGF-H32
SUBJECT_SAMPLE_FACTORS           	6077	FGF-H33	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.021; RAW_FILE_NAME(raw file name)=FGF-H33
SUBJECT_SAMPLE_FACTORS           	5974	FGF-H34	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.021; RAW_FILE_NAME(raw file name)=FGF-H34
SUBJECT_SAMPLE_FACTORS           	6188	FGF-H35	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	Surgery=TAC; weight (g)=0.02; RAW_FILE_NAME(raw file name)=FGF-H35
#COLLECTION
CO:COLLECTION_SUMMARY            	Collection and Storage: Hearts were perfused with ice-cold PBS, carefully
CO:COLLECTION_SUMMARY            	dissected, and snap-frozen in liquid nitrogen. All samples were stored at -80°C
CO:COLLECTION_SUMMARY            	until further processing.
CO:SAMPLE_TYPE                   	Heart
#TREATMENT
TR:TREATMENT_SUMMARY             	Mice underwent Sham or TAC (Transverse Aortic Constriction) surgery. A
TR:TREATMENT_SUMMARY             	thoracotomy was performed, and the transverse aorta was isolated and tied around
TR:TREATMENT_SUMMARY             	27-gauge needle, which was removed to generate the desired constriction. The
TR:TREATMENT_SUMMARY             	incision to the rib cage was closed using a 6.0 silk suture (AD Surgical) and
TR:TREATMENT_SUMMARY             	the skin wound was closed with a 5.0 silk suture. (AD Surgical). The sham
TR:TREATMENT_SUMMARY             	procedure was identical except that aortic arch was not constricted. Tissues
TR:TREATMENT_SUMMARY             	were collected 3 days, 2 weeks or 8 weeks post-surgery.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Each individual sample was homogenized using a cryogenic ball-mill homogenizer
SP:SAMPLEPREP_SUMMARY            	(Cryomill, Retsch Inc.) and weighted into bead tubes (2.8 mm, MO BIO, catalog
SP:SAMPLEPREP_SUMMARY            	#13114-50) for extraction. Based on the weights of tissues, the solvent volumes
SP:SAMPLEPREP_SUMMARY            	used for extraction were calculated for each sample. Modified Bligh and Dyer
SP:SAMPLEPREP_SUMMARY            	extraction1-3 was used to obtain polar metabolites. Briefly, cold methanol and
SP:SAMPLEPREP_SUMMARY            	water were added to the samples in bead tubes and homogenized for 30 s. The
SP:SAMPLEPREP_SUMMARY            	samples were transferred into glass tubes containing cold chloroform and water.
SP:SAMPLEPREP_SUMMARY            	The final methanol:chloroform:water ratio is 2:2:1.8. The mixture was vortexed,
SP:SAMPLEPREP_SUMMARY            	incubated on ice for 10 min, then centrifuged at 2000x gn for 5 min. The polar
SP:SAMPLEPREP_SUMMARY            	phase was transferred into 1.5mL Eppendorf tube and dried by vacuum centrifuge
SP:SAMPLEPREP_SUMMARY            	for 4-5 hrs at room temperature. The dried metabolites were resuspended in 220
SP:SAMPLEPREP_SUMMARY            	uL of NMR buffer containing 100mM phosphate buffer, pH7.3, 1mM TMSP
SP:SAMPLEPREP_SUMMARY            	(3-Trimethylsilyl 2,2,3,3-d4 propinoate), 1mg/mL sodium azide) prepared in D2O.
SP:SAMPLEPREP_SUMMARY            	Final volume of 200 uL of each sample was transferred into 3 mm NMR tube
SP:SAMPLEPREP_SUMMARY            	(Bruker) for data collection
#CHROMATOGRAPHY
#ANALYSIS
AN:ANALYSIS_TYPE                 	NMR
#NMR
NM:INSTRUMENT_NAME               	Bruker Avance III
NM:INSTRUMENT_TYPE               	FT-NMR
NM:NMR_EXPERIMENT_TYPE           	1D-1H
NM:FIELD_FREQUENCY_LOCK          	Detuterium
NM:STANDARD_CONCENTRATION        	1 mM
NM:SPECTROMETER_FREQUENCY        	600 MHz
NM:NMR_PROBE                     	Prodigy 5mm BBI
NM:NMR_SOLVENT                   	D2O
NM:NMR_TUBE_SIZE                 	103.5 mm x 5 mm
NM:SHIMMING_METHOD               	Topshim
NM:PULSE_SEQUENCE                	noesygppr1d
#NMR_METABOLITE_DATA
NMR_METABOLITE_DATA:UNITS        	mM
NMR_METABOLITE_DATA_START
Samples	FGF-H36	FGF-H37	FGF-H38	FGF-H39	FGF-H40	FGF-H11	FGF-H12	FGF-H13	FGF-H14	FGF-H15	FGF-H16	FGF-H17	FGF-H18	FGF-H19	FGF-H20	FGF-H01	FGF-H02	FGF-H04	FGF-H05	FGF-H06	FGF-H07	FGF-H08	FGF-H09	FGF-H10	FGF-H21	FGF-H22	FGF-H23	FGF-H24	FGF-H25	FGF-H26	FGF-H27	FGF-H28	FGF-H29	FGF-H30	FGF-H03	FGF-H31	FGF-H32	FGF-H33	FGF-H34	FGF-H35
Factors	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Sample source:Heart | Genotype:Alb-Cre Fgf21-/- | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:2 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:3 Days	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:Fgf21 fl/fl | Timepoint:8 Weeks	Sample source:Heart | Genotype:- | Timepoint:Fgf21 fl/fl	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	Sample source:Heart | Genotype:αMHC-Cre Fgf21-/- | Timepoint:8 Weeks	
3-Hydroxybutyrate	0.0436	0.0191	0.0312	0.0500	0.0138	0.0300	0.0221	0.0411	0.1400	0.1217	0.0733	0.1160	0.0728	0.0924	0.0809	0.0323	0.0290	0.0273	0.0771	0.0657	0.0651	0.0439	0.1007	0.0514	0.0273	0.0218	0.0300	0.0209	0.0245	0.0557	0.0223	0.0269	0.0658	0.0575	0.0300	0.0369	0.0159	0.0629	0.0371	0.0330
Acetate	0.1636	0.1200	0.0936	0.1850	0.1062	0.2209	0.1089	0.1577	0.1950	0.1283	0.0989	0.1040	0.0748	0.0989	0.1116	0.1662	0.1283	0.1091	0.2229	0.2143	0.1354	0.0937	0.1457	0.1943	0.1500	0.1336	0.1550	0.1200	0.1336	0.0900	0.0943	0.0910	0.0774	0.1275	0.2070	0.1223	0.0847	0.0857	0.1229	0.1680
ADP	1.3745	1.6664	1.7568	2.0350	1.0731	1.1973	0.9347	1.0046	0.3175	0.2800	0.8878	0.8253	0.8882	0.8741	0.8233	1.0892	1.1897	0.8673	0.7829	0.8314	1.2531	0.7259	0.8207	0.6371	1.2082	1.3445	1.2800	1.4426	1.2382	1.4079	1.0560	1.4048	1.2929	1.2375	1.5420	1.0731	1.1559	0.8286	1.1143	1.6860
Alanine	2.8527	1.8655	2.1168	3.0100	0.8492	2.5364	1.6784	2.1789	2.1850	1.9217	2.5756	2.2667	2.3508	2.2800	2.9344	2.4415	1.9924	2.4436	2.1914	2.3857	2.4583	1.8337	3.2507	2.2343	1.3309	0.9273	1.4350	1.0122	1.0336	1.9371	1.0680	1.4400	1.8368	1.9250	2.3790	1.7100	1.9853	2.0114	1.6571	2.7270
AMP	3.2782	3.0027	2.8776	3.1050	2.5708	3.1664	2.3400	3.0017	2.1875	1.6133	2.5722	2.3227	2.3508	2.6546	2.1935	3.7246	3.8338	4.2909	4.3000	2.1086	2.2697	2.0868	2.0893	2.2371	3.4255	3.5945	3.7750	3.3391	2.6809	3.1671	2.6726	3.0393	3.2439	3.6625	2.7840	2.7092	2.6876	2.4371	2.4171	3.4320
Asparagine	0.3436	0.2100	0.2640	0.3650	0.1500	0.1473	0.1326	0.2126	0.1975	0.1183	0.7433	0.2120	0.2302	0.2222	0.2553	0.2423	0.1283	0.2873	0.2029	0.1600	0.2229	0.2195	0.3214	0.1543	0.1473	0.1446	0.2000	0.1696	0.1582	0.2507	0.1783	0.3124	0.2923	0.2775	0.2160	0.2977	0.2294	0.2629	0.3171	0.3090
Aspartate	2.6836	1.6664	1.5264	2.2600	0.7038	1.1264	0.7532	0.8554	0.8525	0.9450	2.6356	1.6227	1.8708	1.1384	1.2586	1.3869	1.2414	1.5455	1.2771	1.2371	1.5686	1.0946	2.2371	1.3800	0.5291	0.8073	0.8950	0.8765	0.9355	1.1614	0.5246	1.1586	1.7419	1.3025	1.4100	3.0946	1.4241	1.6029	2.0000	2.1210
ATP	2.0564	1.8382	1.3704	1.8600	1.1723	1.4209	1.3547	1.0937	0.1050	0.1167	0.8967	1.1120	1.0200	0.7654	1.1609	1.8046	1.3779	0.3018	0.2200	1.8229	0.9051	1.2117	1.0007	1.1686	1.5327	1.4646	2.1500	1.5652	1.1727	1.0479	0.9960	1.4255	1.1710	0.9825	2.3400	1.0177	1.6924	0.8086	0.6257	1.4820
Choline	0.0818	0.0873	0.0672	0.0750	0.0531	0.1064	0.0916	0.0857	0.1350	0.1050	0.0889	0.0573	0.0482	0.0876	0.0544	0.0877	0.0993	0.1055	0.1400	0.0514	0.0737	0.0527	0.0707	0.0686	0.0627	0.1009	0.0900	0.0626	0.0954	0.0814	0.0703	0.0621	0.0736	0.0775	0.1080	0.0669	0.0741	0.0486	0.0714	0.0960
Citrate	0.9327	0.9573	0.9408	1.1150	0.5262	0.8673	0.8368	0.7937	0.4350	0.3600	0.4011	0.4107	0.3846	0.3227	0.4702	0.7477	0.8669	0.6364	0.6429	0.3714	0.4303	0.3424	0.6943	0.4343	0.6900	0.6382	0.3850	0.6287	0.5891	0.7264	0.3223	0.4759	0.6000	0.6000	0.7830	0.5215	0.7059	0.3886	0.6714	0.7170
Creatine	16.4618	13.7755	13.4904	14.7350	10.6800	12.9055	9.7374	12.3223	8.7475	5.7100	9.2611	10.2960	9.0256	9.8092	8.9679	14.7023	14.2614	13.4436	13.3914	10.0200	10.1503	8.3576	6.5700	8.3657	12.6436	12.6709	14.8550	14.2174	11.2554	12.6321	11.1017	11.4972	12.2245	12.2050	13.7220	9.6162	12.0300	9.2514	8.5571	13.3020
Creatine phosphate	0.1855	0.3436	0.1704	0.1700	0.2585	0.2864	0.3174	0.2914	0.1200	0.0383	0.1589	0.2973	0.2193	0.1865	0.2414	0.2977	0.4034	0.1218	0.0543	0.1000	0.1251	0.2327	0.2036	0.1229	0.1909	0.0245	0.1350	0.2791	0.1827	0.1607	0.2211	0.2193	0.1993	0.1400	0.3180	0.1523	0.2576	0.1086	0.0743	0.2130
Creatinine	0.1855	0.1582	0.0984	0.1600	0.1015	0.1227	0.0932	0.1286	0.1025	0.0800	0.0911	0.0907	0.0974	0.0859	0.0767	0.1546	0.1014	0.1400	0.1086	0.1086	0.0840	0.0790	0.1200	0.0829	0.1446	0.1418	0.1450	0.1461	0.0791	0.1329	0.1200	0.1345	0.1239	0.1325	0.0240	0.1062	0.1059	0.1057	0.0914	0.1680
Formate	0.0655	0.0573	0.0504	0.1000	0.0646	0.1064	0.0584	0.0823	0.0925	0.0583	0.0533	0.0693	0.0511	0.0568	0.0544	0.1154	0.0848	0.0818	0.1057	0.1029	0.0771	0.0615	0.1071	0.0800	0.0900	0.0900	0.1500	0.0704	0.0791	0.0793	0.0703	0.0745	0.0716	0.0975	0.1110	0.0531	0.0335	0.0771	0.0657	0.0780
Fumarate	0.2073	0.1582	0.1776	0.2150	0.0831	0.2100	0.1263	0.1526	0.1350	0.0850	0.1544	0.1760	0.1672	0.1411	0.1605	0.2562	0.2007	0.2036	0.1686	0.1629	0.1646	0.1361	0.2014	0.1857	0.1145	0.1336	0.0800	0.0991	0.0846	0.1114	0.0566	0.0828	0.1103	0.1100	0.2820	0.1546	0.1306	0.1257	0.1086	0.2040
Glucose	2.3509	0.6900	1.1232	2.2100	0.3185	4.8573	1.6011	2.0006	0.7475	1.5217	0.1756	0.0920	0.0748	0.2578	0.1828	2.7208	3.0890	1.6018	0.3171	0.1429	0.1114	0.1039	0.3043	0.1000	0.5454	0.2509	0.1700	0.4278	0.2373	0.3814	0.4046	0.2524	0.1258	0.1575	3.2340	0.0992	0.7006	0.1600	0.1314	0.6090
Glutamate	3.7255	3.3355	3.8688	4.2150	3.7177	3.8264	3.8116	5.1171	2.7000	1.8283	3.9944	3.0067	2.9184	2.6805	2.7837	4.2738	4.2745	3.9145	4.0600	2.6771	2.9571	2.1146	3.5614	2.4629	5.2336	3.7500	5.9400	4.8809	3.4718	3.2507	4.3217	4.4297	4.5658	3.4575	4.0980	3.6438	3.1871	2.5429	2.3971	3.2310
Glutamine	6.5400	5.6864	6.4560	5.5250	3.2654	5.8364	4.3389	5.9349	4.4100	2.9267	6.8711	4.9093	4.4016	4.0946	5.4488	5.8177	6.4883	7.2000	6.9857	4.9114	5.2183	5.6810	5.0893	3.9971	5.8445	8.0400	8.3850	5.7991	4.3827	6.4393	5.5611	5.3524	4.5736	5.1825	4.9200	5.8754	5.4671	4.8571	6.8171	7.0440
Glutathione	0.4145	0.4664	0.4944	0.4400	0.3208	0.4200	0.3205	0.4234	0.2575	0.2117	0.4000	0.3507	0.3374	0.3422	0.3181	0.5031	0.4572	0.4891	0.3400	0.3343	0.2914	0.3615	0.4393	0.2771	0.2754	0.3654	0.4600	0.3887	0.3027	0.4800	0.3977	0.5007	0.5284	0.5350	0.3960	0.5308	0.4676	0.3714	0.4543	0.4590
Glycine	0.7636	0.6845	0.6672	0.6400	0.4223	0.5727	0.5668	0.5983	0.8175	0.6383	0.8067	0.5227	0.5823	0.5319	0.5679	0.5654	0.5545	0.6745	0.5914	0.4514	0.4457	0.6029	0.6857	0.3343	0.5836	0.3846	0.5350	0.5061	0.4882	0.7071	0.5709	0.5855	0.6213	0.6125	0.5880	0.6531	0.6212	0.4943	0.8086	0.5910
GTP	0.1145	0.1473	0.1320	0.1400	0.1062	0.1118	0.0900	0.0977	0.0950	0.0533	0.0967	0.0853	0.1033	0.0989	0.0977	0.1177	0.1055	0.1164	0.0800	0.0800	0.0977	0.0907	0.1157	0.0943	0.1255	0.1336	0.1250	0.1200	0.1091	0.1350	0.1097	0.1345	0.1510	0.1500	0.1110	0.1408	0.1076	0.1257	0.1571	0.1380
Histidine	0.1964	0.1936	0.1440	0.1750	0.1846	0.2127	0.1737	0.2074	0.1700	0.0917	0.2889	0.1853	0.1554	0.1816	0.1340	0.2654	0.2317	0.2309	0.2429	0.1543	0.1697	0.2034	0.1779	0.1486	0.1855	0.1936	0.1700	0.2426	0.1991	0.1671	0.1440	0.1614	0.1626	0.1900	0.2520	0.1338	0.1624	0.0914	0.1714	0.1650
IMP	0.1309	0.0409	0.0408	0.0400	0.0600	0.0764	0.1784	0.2537	0.3325	0.1017	0.0467	0.0573	0.0403	0.0535	0.0391	0.0738	0.1076	0.6036	0.4229	0.0429	0.0446	0.0337	0.0471	0.0400	0.0900	0.0846	0.1400	0.0887	0.0791	0.0450	0.0754	0.0579	0.0523	0.0400	0.0690	0.0992	0.0688	0.0600	0.0400	0.0570
Inosine	0.1582	0.0682	0.0768	0.0800	0.0646	0.0573	0.0947	0.1389	0.3675	0.4317	0.1778	0.3493	0.1387	0.2043	0.1493	0.0969	0.0621	0.1455	0.2486	0.2000	0.1663	0.0776	0.1243	0.1571	0.0818	0.1200	0.1150	0.0965	0.0573	0.0793	0.0703	0.0724	0.0368	0.0275	0.0660	0.0577	0.0618	0.2114	0.1743	0.1050
Isoleucine	0.0709	0.0436	0.0648	0.0850	0.0254	0.0736	0.0489	0.0651	0.0900	0.0733	0.0744	0.0640	0.0807	0.0714	0.0977	0.0669	0.0600	0.0618	0.0829	0.0543	0.0566	0.0541	0.1114	0.0514	0.0327	0.0273	0.0250	0.0339	0.0273	0.0771	0.0343	0.0393	0.0639	0.0525	0.0570	0.0900	0.0512	0.0857	0.0743	0.0900
Lactate	10.9473	5.3973	5.5440	6.6750	6.7638	8.2582	6.6553	11.5937	9.6975	9.8600	10.1189	8.1707	9.8066	11.2962	10.8349	9.1523	7.8434	8.8600	10.4314	10.9857	8.8800	8.3210	9.7243	5.6114	9.7964	7.0336	11.3300	8.2487	4.0827	9.4929	8.9863	8.0090	9.2942	9.2125	6.4950	7.5831	5.1600	8.0800	4.9943	9.2910
Leucine	0.1309	0.1091	0.1080	0.1550	0.0369	0.1200	0.1011	0.1183	0.1575	0.1333	0.1567	0.1227	0.1397	0.1508	0.1437	0.1038	0.0972	0.1145	0.1400	0.1114	0.1217	0.1127	0.2036	0.0914	0.0464	0.0327	0.0450	0.0444	0.0355	0.1436	0.0583	0.0745	0.1181	0.1025	0.1140	0.1385	0.0865	0.1314	0.1343	0.1530
Lysine	0.2291	0.2482	0.1392	0.4650	0.2792	0.2018	0.1468	0.3051	0.2225	0.1300	0.1378	0.2080	0.1495	0.1654	0.1800	0.1938	0.2938	0.4073	0.4000	0.2257	0.1663	0.1800	0.2829	0.2657	0.2455	0.3000	0.2500	0.2661	0.1609	0.2293	0.1440	0.2090	0.2168	0.1725	0.1950	0.1754	0.2400	0.1800	0.1971	0.1890
Methionine	0.2891	0.2427	0.1320	0.1800	0.1315	0.0955	0.0821	0.1371	0.0650	0.0500	0.1611	0.1267	0.1328	0.1249	0.1102	0.0877	0.0931	0.1000	0.0743	0.1114	0.1474	0.1420	0.1693	0.0914	0.1773	0.1227	0.0700	0.1122	0.0300	0.2421	0.1440	0.1572	0.1916	0.2575	0.0930	0.1385	0.1094	0.2286	0.2343	0.1410
myo-Inositol	0.3709	0.3191	0.2880	0.4150	0.2538	0.3791	0.4232	0.4629	0.3850	0.6833	0.2767	0.3173	0.3452	0.3843	0.2791	0.4223	0.3724	0.5545	0.5114	0.2143	0.2451	0.2078	0.2764	0.2429	0.4582	0.3736	0.4050	0.3078	0.3327	0.3450	0.3309	0.2710	0.3426	0.3700	0.3870	0.3046	0.2294	0.3029	0.3314	0.4140
NAD+	0.5236	0.5427	0.5328	0.6650	0.5008	0.7582	0.6379	0.6634	0.1850	0.2750	0.5378	0.6573	0.5538	0.5854	0.5512	0.9254	0.7034	0.6455	0.6686	0.7314	0.5897	0.5356	0.6279	0.5286	0.6218	0.4882	0.6050	0.5974	0.5454	0.4521	0.4440	0.4779	0.4335	0.4875	0.7770	0.3392	0.5912	0.3600	0.3314	0.6030
NADP+	0.0382	0.0600	0.0528	0.0650	0.0554	0.0709	0.0600	0.0514	0.0400	0.0317	0.0422	0.0413	0.0384	0.0551	0.0530	0.0531	0.0641	0.0545	0.0429	0.0429	0.0497	0.0454	0.0621	0.0457	0.0300	0.0491	0.0500	0.0522	0.0409	0.0493	0.0429	0.0435	0.0600	0.0475	0.0840	0.0508	0.0547	0.0286	0.0400	0.0630
Niacinamide	0.2673	0.2073	0.1800	0.2900	0.1823	0.2209	0.1942	0.2983	0.4250	0.1867	0.1567	0.1547	0.1466	0.1524	0.1312	0.3300	0.2938	0.3545	0.2914	0.1343	0.1406	0.1595	0.1629	0.1657	0.2373	0.2346	0.2650	0.2426	0.1964	0.2143	0.2057	0.1490	0.1935	0.2175	0.2400	0.2146	0.1694	0.2571	0.2257	0.2310
N_N-Dimethylglycine	0.0436	0.0382	0.0336	0.0400	0.0531	0.0300	0.0253	0.0291	0.0200	0.0117	0.0222	0.0240	0.0207	0.0243	0.0209	0.0346	0.0310	0.0309	0.0314	0.0257	0.0257	0.0205	0.0171	0.0200	0.0300	0.0300	0.0350	0.0365	0.0273	0.0321	0.0257	0.0269	0.0310	0.0325	0.0330	0.0254	0.0300	0.0257	0.0229	0.0330
O-Acetylcarnitine	0.1909	0.1173	0.1464	0.1400	0.0969	0.1200	0.1263	0.2160	0.1000	0.1000	0.1867	0.2053	0.1603	0.2173	0.1730	0.1408	0.1262	0.0600	0.1314	0.1457	0.1869	0.1390	0.1329	0.1286	0.1746	0.1036	0.1600	0.1278	0.0654	0.2250	0.1217	0.1779	0.3135	0.2525	0.0780	0.1177	0.0565	0.1486	0.1171	0.0840
O-Phosphocholine	0.2018	0.1309	0.1608	0.1800	0.1131	0.1418	0.1263	0.1509	0.1100	0.1450	0.1911	0.1387	0.1348	0.1103	0.1047	0.1777	0.1655	0.1727	0.1457	0.0943	0.0994	0.0732	0.0943	0.1029	0.1391	0.1555	0.1400	0.1487	0.0982	0.1350	0.1080	0.1469	0.1548	0.1425	0.1680	0.1638	0.1076	0.1314	0.1457	0.1770
O-Phosphoethanolamine	0.2727	0.2618	0.2232	0.4150	0.2885	0.1827	0.2337	0.2657	0.3175	0.2000	0.1733	0.1827	0.2075	0.1995	0.2833	0.3600	0.1759	0.4145	0.3229	0.1886	0.1371	0.1551	0.3879	0.1629	0.2127	0.1964	0.2550	0.3183	0.2809	0.1350	0.2126	0.1593	0.1626	0.2025	0.2490	0.3300	0.1976	0.2314	0.2486	0.2850
Pantothenate	0.0655	0.0600	0.0456	0.0950	0.0438	0.0600	0.0474	0.0480	0.0600	0.0450	0.0411	0.0413	0.0334	0.0632	0.0279	0.0669	0.0455	0.0745	0.0886	0.0571	0.0463	0.0556	0.0643	0.0629	0.0436	0.0873	0.1250	0.0600	0.0436	0.0514	0.0497	0.0352	0.0368	0.0350	0.0450	0.0715	0.0459	0.0429	0.0400	0.0780
Phenylalanine	0.0382	0.0518	0.0336	0.0650	0.0577	0.0491	0.0395	0.0549	0.0550	0.0550	0.0944	0.0547	0.0423	0.0600	0.0447	0.0623	0.0476	0.0636	0.0771	0.0286	0.0463	0.0454	0.0621	0.0314	0.0464	0.0546	0.0600	0.0470	0.0382	0.0643	0.0566	0.0538	0.0755	0.0550	0.0690	0.0577	0.0371	0.0400	0.0543	0.0840
Pyruvate	0.2018	0.1391	0.1536	0.2050	0.0715	0.1500	0.0805	0.0926	0.1050	0.0750	0.1011	0.1133	0.0875	0.0892	0.1074	0.1915	0.1490	0.1527	0.1343	0.1229	0.1149	0.0937	0.1371	0.1086	0.0846	0.0954	0.0600	0.0783	0.0627	0.1136	0.0429	0.0848	0.0871	0.0925	0.1770	0.1246	0.1182	0.1171	0.1000	0.1890
Serine	0.6164	0.6545	0.5328	0.6650	0.3646	0.4664	0.3742	0.5366	0.4625	0.4617	1.1222	0.5867	0.6108	0.7135	0.6028	0.4708	0.4883	0.5982	0.6229	0.4314	0.6274	0.7259	1.1679	0.4800	0.3736	0.3164	0.3250	0.1983	0.3355	0.5143	0.4423	0.6228	0.5961	0.4450	0.3780	0.5908	0.3512	0.5000	0.7429	0.5610
sn-Glycero-3-phosphocholine	0.6491	0.4636	0.5112	0.4600	0.4685	0.8182	0.6726	0.5863	0.4950	0.3400	0.5744	0.4987	0.4820	0.5870	0.5149	0.9854	1.0697	0.8000	0.7686	0.6971	0.6206	0.6132	0.4800	0.4429	0.5782	0.5182	0.7050	0.7278	0.3791	0.6236	0.4869	0.5090	0.4839	0.5550	0.9750	0.4477	0.3812	0.3686	0.4000	0.4710
Succinate	0.6218	0.2700	0.3096	0.4450	0.3831	0.5536	0.3363	0.6377	0.6450	0.7083	0.7544	0.9840	0.7810	0.9405	0.9851	0.7292	0.5338	0.8782	1.1029	1.0171	0.9737	0.7624	0.6686	0.7057	0.4555	0.3927	0.4450	0.4252	0.2836	0.3879	0.2194	0.4448	0.6774	0.6600	0.4050	0.3023	0.3335	0.5314	0.2971	0.5040
Taurine	43.1673	39.3818	37.5840	38.6550	32.3954	36.2836	30.6000	35.9451	24.7300	20.0417	26.1244	29.2200	28.1282	28.3411	29.3847	40.4308	40.0490	37.8782	38.6829	31.8886	30.0223	29.8537	31.0114	25.3029	37.2600	36.8700	35.6100	41.9922	33.2454	41.6421	34.4571	36.5628	39.8477	39.4075	38.6010	31.5392	32.5729	25.9543	31.1771	37.0800
Threonine	0.2945	0.1827	0.1344	0.2350	0.1777	0.2673	0.2289	0.1971	0.1825	0.1167	0.3033	0.3053	0.2980	0.2578	0.2749	0.3185	0.1903	0.2636	0.3800	0.2914	0.3240	0.2371	0.4329	0.2286	0.2346	0.1991	0.2300	0.2400	0.2155	0.2721	0.2194	0.2566	0.2884	0.2875	0.1680	0.4015	0.2453	0.2229	0.2657	0.4080
Tyrosine	0.0764	0.0764	0.0576	0.1000	0.0600	0.0818	0.0537	0.0891	0.0650	0.0800	0.1078	0.0733	0.0856	0.0924	0.0809	0.0808	0.0724	0.1091	0.0714	0.0914	0.0840	0.0717	0.0964	0.0486	0.0573	0.0518	0.0550	0.0626	0.0518	0.0836	0.0531	0.0579	0.0929	0.0775	0.0690	0.0600	0.0406	0.0686	0.0600	0.0750
UDP-glucose	0.0764	0.0818	0.0432	0.0750	0.0462	0.0682	0.0616	0.0686	0.1000	0.0850	0.1000	0.0640	0.0669	0.0665	0.0767	0.0692	0.0331	0.0655	0.0371	0.0743	0.0737	0.0907	0.1457	0.0657	0.0818	0.0818	0.0450	0.0991	0.0573	0.0986	0.0651	0.0559	0.1123	0.0675	0.0810	0.0462	0.0653	0.0829	0.0343	0.0480
UDP-N-Acetylglucosamine	0.0436	0.0491	0.0408	0.0600	0.0323	0.0409	0.0253	0.0291	0.0225	0.0250	0.0333	0.0213	0.0256	0.0389	0.0363	0.0462	0.0372	0.0218	0.0314	0.0371	0.0326	0.0410	0.0171	0.0229	0.0436	0.0409	0.0900	0.0287	0.0464	0.0514	0.0206	0.0393	0.0252	0.0425	0.0570	0.0438	0.0318	0.0257	0.0657	0.0360
Valine	0.1473	0.0927	0.1128	0.1500	0.0462	0.1445	0.1074	0.1389	0.1450	0.1350	0.1678	0.1373	0.1525	0.1297	0.1786	0.1385	0.1345	0.1164	0.1543	0.1286	0.1354	0.1244	0.1821	0.0943	0.0682	0.0382	0.0350	0.0626	0.0518	0.1329	0.0754	0.0890	0.1355	0.1250	0.1230	0.1454	0.0988	0.1257	0.1343	0.1560
NMR_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	pubchem_id	inchi_key	kegg_id	other_id	other_id_type	ri	ri_type	moverz_quant	
3-Hydroxybutyrate									
Acetate									
ADP									
Alanine									
AMP									
Asparagine									
Aspartate									
ATP									
Choline									
Citrate									
Creatine									
Creatine phosphate									
Creatinine									
Formate									
Fumarate									
Glucose									
Glutamate									
Glutamine									
Glutathione									
Glycine									
GTP									
Histidine									
IMP									
Inosine									
Isoleucine									
Lactate									
Leucine									
Lysine									
Methionine									
myo-Inositol									
NAD+									
NADP+									
Niacinamide									
N,N-Dimethylglycine									
O-Acetylcarnitine									
O-Phosphocholine									
O-Phosphoethanolamine									
Pantothenate									
Phenylalanine									
Pyruvate									
Serine									
sn-Glycero-3-phosphocholine									
Succinate									
Taurine									
Threonine									
Tyrosine									
UDP-glucose									
UDP-N-Acetylglucosamine									
Valine									
METABOLITES_END
#END