#METABOLOMICS WORKBENCH nozomu_kono_20250125_005607 DATATRACK_ID:5568 STUDY_ID:ST003747 ANALYSIS_ID:AN006153 PROJECT_ID:PR002333
VERSION             	1
CREATED_ON             	February 24, 2025, 5:03 pm
#PROJECT
PR:PROJECT_TITLE                 	Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures
PR:PROJECT_TITLE                 	embryonic radial glial cell integrity
PR:PROJECT_SUMMARY               	Arachidonic acid, a vital polyunsaturated fatty acid in brain development, is
PR:PROJECT_SUMMARY               	enriched in phosphatidylinositol (PI). The arachidonic acyl chain in PI is
PR:PROJECT_SUMMARY               	introduced by lysophospholipid acyltransferase 11 (LPLAT11)/membrane-bound
PR:PROJECT_SUMMARY               	O-acyltransferase 7 (MBOAT7), the loss of which causes microcephaly in humans
PR:PROJECT_SUMMARY               	and mice. Here, we show that LPLAT11 deficiency impaired indirect neurogenesis
PR:PROJECT_SUMMARY               	in the developing neocortex, resulting in fewer layer II-V neurons.
PR:PROJECT_SUMMARY               	LPLAT11-deficient radial glial cells had defects in differentiation into
PR:PROJECT_SUMMARY               	intermediate progenitor cells and increased apoptosis. Prior to these anomalies,
PR:PROJECT_SUMMARY               	LPLAT11 deficiency caused a rounding of the Golgi apparatus, accompanied by
PR:PROJECT_SUMMARY               	impaired apical trafficking of E-cadherin, and deregulated apical detachment.
PR:PROJECT_SUMMARY               	Moreover, impaired PI acyl chain remodeling led to a decreased amount of
PR:PROJECT_SUMMARY               	PI(4,5)P2, leading to Golgi apparatus rounding. Thus, these results clarify the
PR:PROJECT_SUMMARY               	underlying mechanism of microcephaly by LPLAT11 deficiency and highlight the
PR:PROJECT_SUMMARY               	critical role of arachidonic acid in PI in the integrity of radial glial cells.
PR:INSTITUTE                     	University of Tokyo
PR:DEPARTMENT                    	Graduate School of Pharmaceutical Sciences
PR:LABORATORY                    	Department of Health Chemistry
PR:LAST_NAME                     	Kono
PR:FIRST_NAME                    	Nozomu
PR:ADDRESS                       	Hongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan
PR:EMAIL                         	nozomu@mol.f.u-tokyo.ac.jp
PR:PHONE                         	81-3-5841-4723
#STUDY
ST:STUDY_TITLE                   	Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures
ST:STUDY_TITLE                   	embryonic radial glial cell integrity - part 1
ST:STUDY_SUMMARY                 	Since fragmentation of the Golgi apparatus is associated with defects in
ST:STUDY_SUMMARY                 	phosphoinositide (PIP) metabolisms, we measured PI in E11.5-E13.5 cortices by
ST:STUDY_SUMMARY                 	using liquid chromatography-mass spectrometry (LC-MS). In Mboat7 KO mice, PI
ST:STUDY_SUMMARY                 	with arachidonic acid (38:4 PI), which is the major species in Mboat7
ST:STUDY_SUMMARY                 	heterozygous mice, significantly decreased, and instead, PI with monounsaturated
ST:STUDY_SUMMARY                 	fatty acid (34:1, 34:2, 36:1, 36:2 PI) and PI with docosapentaenoic acid (DPA)
ST:STUDY_SUMMARY                 	or docosahexaenoic acid (DHA) (38:6, 40:5, 40:6, 40:7 PI) increased. On the
ST:STUDY_SUMMARY                 	other hand, the composition of molecular species of phosphatidylserine (PS),
ST:STUDY_SUMMARY                 	phosphatidylcholine (PC), and phosphatidylethanolamine (PE) showed little or no
ST:STUDY_SUMMARY                 	change in the cortices of E11.5-E13.5 Mboat7 KO mice compared with Mboat7
ST:STUDY_SUMMARY                 	heterozygous mice.
ST:INSTITUTE                     	University of Tokyo
ST:LAST_NAME                     	Kono
ST:FIRST_NAME                    	Nozomu
ST:ADDRESS                       	Hongo 7-3-1, Bunkyo-ku, Tokyo 113-033 JAPAN
ST:EMAIL                         	nozomu@mol.f.u-tokyo.ac.jp
ST:PHONE                         	81-3-5841-4723
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_1_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_2_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_3_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_4_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_1_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_2_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_3_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_4_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_5_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_1_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_2_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_3_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_4_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_1_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_2_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_3_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_4_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_5_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_1_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_2_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_3_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_4_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_1_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_2_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_3_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_4_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_5_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_1_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_2_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_3_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_4_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_1_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_2_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_3_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_4_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_5_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_1_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_2_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_3_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_4_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_5_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_1_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_2_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_3_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_1_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_2_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_3_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_4_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_5_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_1_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_2_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_3_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS KO 3.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Embryos at different gestational ages were collected from Mboat7 heterozygous
CO:COLLECTION_SUMMARY            	intercrosses, and the cortices were subsequently dissected from the embryos.
CO:SAMPLE_TYPE                   	Brain cortex
#TREATMENT
TR:TREATMENT_SUMMARY             	Not applicable.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipid extractions were conducted by the method of Bligh and Dyer. The extracted
SP:SAMPLEPREP_SUMMARY            	solutions were dried up with a centrifugal evaporator, dissolved in
SP:SAMPLEPREP_SUMMARY            	chloroform/methanol (2/1, v/v), and stored at -20 ºC. The phospholipid content
SP:SAMPLEPREP_SUMMARY            	of samples was quantified using a phosphorus assay. An aliquot of the extracted
SP:SAMPLEPREP_SUMMARY            	lipids was dried under a stream of nitrogen and dissolved in
SP:SAMPLEPREP_SUMMARY            	isopropanol:methanol (1:1, v/v) containing 0.25 µM internal standards
SP:SAMPLEPREP_SUMMARY            	(12:0/13:0 PC, PE, PS, PI) to achieve a final phospholipid concentration of 300
SP:SAMPLEPREP_SUMMARY            	µM.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Shimadzu Nexera X2
CH:COLUMN_NAME                   	Merck SeQuant ZIC-HILIC (250 x 2.1mm, 1.8um)
CH:SOLVENT_A                     	95% water/5% acetonitrile; 10 mM ammonium acetate
CH:SOLVENT_B                     	50% water/50% acetonitrile; 20 mM ammonium acetate
CH:FLOW_GRADIENT                 	0–22 min: 0% B to 40% B; 22–25 min: 40% B to 40% B; 25–30 min: 0% B
CH:FLOW_RATE                     	0.3 mL/min
CH:COLUMN_TEMPERATURE            	50
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 4500 QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	The instrument parameters for negative ion mode were as follows: curtain gas, 10
MS:MS_COMMENTS                   	psi; collision gas, 7 arb. unit; ionspray voltage, −4500 V; temperature,
MS:MS_COMMENTS                   	700°C; ion source gas 1, 30 psi; ion source gas 2, 70 psi. The specific
MS:MS_COMMENTS                   	detection of PS and PI species was performed using multiple reaction monitoring.
MS:MS_COMMENTS                   	12:0/13:0 PI and PS were used as internal standards. Analyst (SCIEX) was used
MS:MS_COMMENTS                   	for data acquisition. MultiQuant (SCIEX) was used for data quantification.
MS:MS_COMMENTS                   	Gaussian smoothing width was 2.0 points. The peak area of the lipid species was
MS:MS_COMMENTS                   	divided by the corresponding internal standard area, and the total amount of
MS:MS_COMMENTS                   	phospholipids per cortex was calculated from the ratio of injection volume to
MS:MS_COMMENTS                   	total sample volume. The amounts of phospholipid per cortex are shown because
MS:MS_COMMENTS                   	the size of the E11-E13 cortices is not different between genotypes.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	pmol/cortex
MS_METABOLITE_DATA_START
Samples	E11.5_Het_1_PS_PI	E11.5_Het_2_PS_PI	E11.5_Het_3_PS_PI	E11.5_Het_4_PS_PI	E11.5_KO_1_PS_PI	E11.5_KO_2_PS_PI	E11.5_KO_3_PS_PI	E11.5_KO_4_PS_PI	E11.5_KO_5_PS_PI	E12.5_Het_1_PS_PI	E12.5_Het_2_PS_PI	E12.5_Het_3_PS_PI	E12.5_Het_4_PS_PI	E12.5_KO_1_PS_PI	E12.5_KO_2_PS_PI	E12.5_KO_3_PS_PI	E12.5_KO_4_PS_PI	E12.5_KO_5_PS_PI	E13.5_Het_1_PS_PI	E13.5_Het_2_PS_PI	E13.5_Het_3_PS_PI	E13.5_Het_4_PS_PI	E13.5_Het_5_PS_PI	E13.5_KO_1_PS_PI	E13.5_KO_2_PS_PI	E13.5_KO_3_PS_PI
Factors	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-
PI 32:1	5.007656177	6.899725162	4.975433622	5.603463285	17.81706247	22.46997902	28.99988498	21.67598229	23.61084772	16.12689451	19.50951613	12.10829305	22.78144176	94.64280545	69.02640903	114.4822627	70.35891989	109.8289196	26.56880787	27.72158355	26.23855888	26.83022199	27.81066138	180.8995651	230.007159	247.4577249
PI 34:0	5.984489285	8.497158598	6.444927666	10.00054154	15.32219509	14.67493119	17.14544513	18.9207875	16.62491299	11.36632382	17.50640004	10.98638122	17.18892102	62.83494723	45.58465484	80.9715006	46.44529895	71.29245658	14.157102	18.40371207	16.65523761	17.0412792	23.15946818	100.8832787	116.1795789	141.4539718
PI 34:1	30.41419057	26.6238906	32.48521492	33.75647479	91.47847051	65.73034112	114.6624109	108.5136587	103.0223915	65.44247049	92.19550485	50.68296346	93.47638871	382.4411213	276.4459471	516.212578	281.4356796	416.5791652	86.8126793	102.8507386	74.72991461	76.34538718	99.77870876	627.8925061	655.1996761	656.3081047
PI 34:2	32.48469224	35.5195081	28.58446233	22.34321734	53.75631508	138.4589987	128.3931447	76.72637909	104.3241184	54.23606248	84.54819184	46.9890091	82.45784959	354.740788	272.5323709	437.987564	234.8101272	418.1206237	71.92140273	91.81095379	68.48263868	76.61159774	87.06030082	470.3702482	559.4397234	539.5173093
PI 36:1	18.24999327	26.48897224	19.00629857	20.18696675	37.17172906	78.74720965	68.48113642	76.21813928	58.19959286	41.2336769	51.27768545	34.94635983	59.99961832	185.4157114	148.6024582	243.1954955	140.8380087	202.412772	43.75957458	54.31964917	35.99680388	36.75607308	47.32251332	316.6134626	302.0943961	274.2188406
PI 36:2	63.77145137	75.50031679	42.56873934	62.066558	143.1706867	188.8468769	168.7226823	214.1740413	174.2454527	117.8456518	176.7332638	110.4008977	177.4719366	502.6116849	403.1550661	580.6598375	438.2561586	505.5983948	127.649408	169.3825391	125.1954095	108.3857302	155.90182	818.2057519	731.1050931	674.8096168
PI 36:3	44.37528753	63.97828846	17.60076423	41.35911696	57.56741944	93.96047288	59.69634231	90.94817742	77.73169608	116.8615545	201.5635571	119.7700549	181.2916969	226.8983184	173.6720332	289.5141306	191.9098122	256.3638202	245.0480767	253.1578973	230.0246995	273.05598	364.5686283	467.3892493	422.9016091	471.4581754
PI 36:4	52.96786945	113.6912085	101.6248702	82.1698771	43.35804289	82.75239997	53.77990568	88.42481132	57.53013328	305.1931754	571.3054029	292.7712454	532.2199248	397.7849334	298.6795973	491.9202213	282.3970312	451.4546642	773.2381935	729.5539212	784.6578561	1101.73144	1198.88847	948.5852303	944.4649876	1021.547777
PI 38:2	15.11466218	17.87218602	19.51165923	19.87096451	11.60681706	22.33001269	9.812801932	16.3260895	14.75290564	71.71609078	130.9328489	59.30616871	117.5310839	60.95431186	49.9179479	86.38289496	42.08316609	82.25608031	162.0724985	163.887071	158.6808085	161.2855726	228.6997483	117.2787727	149.9017441	150.5725742
PI 38:3	140.128595	167.8384455	164.5580649	128.1667917	45.14692861	103.004451	72.67943409	78.18868312	77.73169608	592.5495872	1134.055831	489.7622526	1005.870193	371.1029947	263.0603822	506.0605483	281.9163554	453.8631932	1443.414901	1285.939536	1366.903972	1660.773628	1846.967588	825.7366965	803.8032389	855.6949364
PI 38:4	406.1141128	443.1618547	542.1572329	494.2646832	160.3415965	371.1261029	358.868185	264.6413633	298.0334886	2510.063177	4568.565302	2287.387248	3986.262595	1592.090235	1146.847979	1840.05537	1141.60502	1760.152948	5169.69713	4805.481553	5021.560388	8254.429034	7345.411326	4033.762202	3230.180221	3677.175538
PI 38:5	140.3504345	203.2770026	244.7840696	296.0197473	79.75199555	123.8163674	107.4016563	99.59717077	93.72434168	853.0893475	1450.17259	828.3051134	1315.368714	400.5006523	343.1468978	441.4320026	332.3272296	443.0729835	1648.429685	1581.473599	1494.348401	1966.155177	2066.982535	952.5075973	791.5850632	837.0282322
PI 38:6	10.10848493	10.99134943	9.593956278	13.48586039	13.3657883	23.99884199	27.34644582	13.78489042	22.58186354	35.00926147	58.96673005	31.18676169	50.93013635	119.5595268	92.62130316	158.3535336	85.80062989	147.6909945	66.28349616	61.37827265	56.68778174	83.24784684	77.48772063	250.403908	264.5235056	280.3309473
PI 40:3	9.006682255	9.579203881	7.170593988	9.703127665	15.71706619	28.14399864	21.05618818	26.84754532	14.15163175	25.49427069	36.12911998	17.2822162	35.15158834	77.26220416	66.8711062	112.8506865	48.35598525	84.92473037	29.39468733	36.63645403	28.55005097	31.12762112	37.24814466	104.0839302	119.3563046	117.2863716
PI 40:4	26.80560195	35.03380199	24.62843591	33.84941663	27.31191746	107.7740728	94.03036577	83.43157805	58.03222796	78.2357354	113.9272279	54.2217598	99.2158233	251.8829327	194.2041286	367.9204309	174.9659904	278.6186276	82.65697421	87.89085321	75.89190793	101.6353909	103.7930166	393.0211713	360.7416398	371.3517794
PI 40:5	38.42259524	48.49865477	38.90487455	49.38929158	70.17936292	99.64525914	92.80825857	90.85901254	76.8018911	102.1000949	124.5687822	76.86289525	137.315483	247.9451402	222.1663469	329.4877473	166.3739105	287.0003083	118.1051386	123.3427286	117.8236239	137.8400278	155.9790182	415.6140051	337.3743787	398.9388555
PI 40:6	23.04912035	30.03282794	21.24094024	29.76927003	29.12473476	83.54913138	80.44340925	85.15246023	63.97058242	71.40856038	107.5631611	51.5900411	110.5771614	290.3782489	212.4674842	366.8327134	172.9231182	331.0282173	92.86615638	103.852135	101.8555867	125.6513869	115.0832573	508.8094446	511.3306563	590.8920439
PI 40:7	7.720013361	9.498252863	5.291284034	9.045099467	22.04696951	32.96745364	26.76414769	28.46142964	20.2697485	23.29850359	37.90271236	16.73916313	32.36022509	114.6033397	76.74012442	128.6225897	80.63336507	121.5825408	35.76676846	35.02445005	34.97225063	39.45620881	39.75708705	183.5667746	200.3780827	195.422222
PS 34:0	271.67805	315.2042325	558.41546	354.491315	272.7815219	522.2687965	358.6915535	368.3655263	341.6657761	303.5590317	597.5600303	285.3064835	526.0699797	378.7026253	273.6227715	364.4256071	261.3850369	336.0379575	561.2723651	478.0950489	551.2464576	723.6478153	752.6133201	588.1987458	470.156015	597.1698336
PS 34:1	809.0024449	1385.893543	1202.940503	1430.578739	734.4914224	1639.825789	1140.177225	930.0153898	1064.345698	1487.751707	2970.727008	1325.197054	2487.001874	1885.803865	1556.600132	2095.370487	1522.070116	1749.555421	3060.462208	2379.503471	2714.978317	3151.844623	4163.715272	3464.317613	2769.739428	3785.587438
PS 36:0	241.393864	204.8687917	277.0031172	258.4974845	246.5127131	264.8017403	87.1040724	169.1237951	163.4897063	246.9571695	529.4683159	246.1198098	444.7242934	315.9611005	234.7607138	345.6977537	234.1547529	277.8478984	498.8730305	428.3106247	462.9785273	618.7827045	746.3620642	669.5085724	463.6711044	586.873802
PS 36:1	1555.174628	1270.58854	1752.278146	1866.269325	1564.741064	2379.843018	1169.400452	1074.151227	1087.622199	2391.458721	4625.817646	2253.577477	3977.475913	2810.915196	2054.28861	3002.59683	2297.876319	2530.561071	4490.178921	3676.915738	4086.478258	5225.581646	6741.884738	6299.349068	4298.813073	5619.71772
PS 36:2	714.5057318	874.4195361	879.5108337	919.2072795	685.8358947	935.4996562	731.2091503	637.6066364	677.0967752	1017.992303	1801.919675	972.5169916	1544.186372	1049.289736	833.8929279	1113.539745	850.6894837	894.9451261	1554.193735	1274.097247	1371.499941	1460.845617	1934.100679	1839.851077	1283.670978	1569.067329
PS 38:3	331.7437798	422.6919469	711.4415266	643.0246463	378.1155631	673.5466548	487.21091	381.10773	444.4703202	662.8787089	1085.851941	615.7120159	1087.717903	662.5220373	482.8637144	763.6973022	529.7060895	552.9982443	944.0311699	861.009959	919.5743647	1101.476417	1415.246442	1096.168913	745.9353689	962.319792
PS 38:4	496.8617066	491.9307863	1095.044158	1130.483052	633.8158891	1155.540177	937.1857717	828.243243	689.1506773	1303.885785	2729.694391	1438.219671	2094.953662	1446.969004	1158.44935	1565.771353	1171.287544	1237.277373	2119.647499	1810.342698	2084.4931	2478.272844	2777.073061	2720.419199	1641.023681	2236.872729
PS 40:3	169.3903845	239.9069947	263.2567078	229.0397555	182.1476614	396.5313558	227.9725993	214.4661824	189.9528706	341.1734327	637.1295517	293.6938066	574.6010579	433.497681	337.4751443	507.0336058	324.8367362	428.6539236	568.5094013	487.4210568	584.5609852	717.1673871	924.4281405	947.8217289	714.5347494	881.6276373
PS 40:4	548.1311998	782.0080206	723.1130063	602.5564013	415.9012388	1212.187514	836.1613876	694.201879	642.320575	1157.994786	2141.173083	1101.489272	1804.803443	1486.108329	1282.342014	1659.41062	1349.954171	1411.719095	1775.486221	1474.332122	1709.315477	2193.526756	2566.803546	3148.593286	1935.233833	2782.322962
PS 40:5	430.7642717	805.4598856	785.1015316	500.8602272	442.0406446	1022.92647	670.7202112	595.7393956	634.2846403	1058.130354	1863.583853	871.0441315	1622.250597	1283.295461	962.6565851	1493.776572	1014.456522	1213.256311	1783.527372	1438.673856	1748.234318	2601.990109	2583.852425	2543.094577	1747.512738	2715.279035
PS 40:6	793.6718611	1367.187889	1385.015586	1668.658155	750.2785882	1518.345084	1162.644545	921.244782	941.8669691	2371.029175	4376.750609	1982.708189	4053.467637	2383.940736	1996.048469	2738.564798	1894.55985	2468.902731	4726.588771	3678.28721	4629.785273	6757.31922	6412.273065	5365.15106	4111.091978	6450.583527
PS 42:5	210.1546581	199.9550676	351.9599532	346.3451099	196.3561107	296.4980534	422.008547	363.8974808	370.2071994	412.1961515	711.046221	326.2806194	675.1173836	598.2386602	506.265662	638.8961149	479.9979768	542.850309	695.5595928	514.1647557	658.6624576	1014.088825	918.5557486	1087.518931	840.8198496	1241.509859
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
PI 32:1
PI 34:0
PI 34:1
PI 34:2
PI 36:1
PI 36:2
PI 36:3
PI 36:4
PI 38:2
PI 38:3
PI 38:4
PI 38:5
PI 38:6
PI 40:3
PI 40:4
PI 40:5
PI 40:6
PI 40:7
PS 34:0
PS 34:1
PS 36:0
PS 36:1
PS 36:2
PS 38:3
PS 38:4
PS 40:3
PS 40:4
PS 40:5
PS 40:6
PS 42:5
METABOLITES_END
#END