#METABOLOMICS WORKBENCH nozomu_kono_20250125_005607 DATATRACK_ID:5568 STUDY_ID:ST003747 ANALYSIS_ID:AN006154 PROJECT_ID:PR002333
VERSION             	1
CREATED_ON             	February 24, 2025, 5:03 pm
#PROJECT
PR:PROJECT_TITLE                 	Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures
PR:PROJECT_TITLE                 	embryonic radial glial cell integrity
PR:PROJECT_SUMMARY               	Arachidonic acid, a vital polyunsaturated fatty acid in brain development, is
PR:PROJECT_SUMMARY               	enriched in phosphatidylinositol (PI). The arachidonic acyl chain in PI is
PR:PROJECT_SUMMARY               	introduced by lysophospholipid acyltransferase 11 (LPLAT11)/membrane-bound
PR:PROJECT_SUMMARY               	O-acyltransferase 7 (MBOAT7), the loss of which causes microcephaly in humans
PR:PROJECT_SUMMARY               	and mice. Here, we show that LPLAT11 deficiency impaired indirect neurogenesis
PR:PROJECT_SUMMARY               	in the developing neocortex, resulting in fewer layer II-V neurons.
PR:PROJECT_SUMMARY               	LPLAT11-deficient radial glial cells had defects in differentiation into
PR:PROJECT_SUMMARY               	intermediate progenitor cells and increased apoptosis. Prior to these anomalies,
PR:PROJECT_SUMMARY               	LPLAT11 deficiency caused a rounding of the Golgi apparatus, accompanied by
PR:PROJECT_SUMMARY               	impaired apical trafficking of E-cadherin, and deregulated apical detachment.
PR:PROJECT_SUMMARY               	Moreover, impaired PI acyl chain remodeling led to a decreased amount of
PR:PROJECT_SUMMARY               	PI(4,5)P2, leading to Golgi apparatus rounding. Thus, these results clarify the
PR:PROJECT_SUMMARY               	underlying mechanism of microcephaly by LPLAT11 deficiency and highlight the
PR:PROJECT_SUMMARY               	critical role of arachidonic acid in PI in the integrity of radial glial cells.
PR:INSTITUTE                     	University of Tokyo
PR:DEPARTMENT                    	Graduate School of Pharmaceutical Sciences
PR:LABORATORY                    	Department of Health Chemistry
PR:LAST_NAME                     	Kono
PR:FIRST_NAME                    	Nozomu
PR:ADDRESS                       	Hongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan
PR:EMAIL                         	nozomu@mol.f.u-tokyo.ac.jp
PR:PHONE                         	81-3-5841-4723
#STUDY
ST:STUDY_TITLE                   	Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures
ST:STUDY_TITLE                   	embryonic radial glial cell integrity - part 1
ST:STUDY_SUMMARY                 	Since fragmentation of the Golgi apparatus is associated with defects in
ST:STUDY_SUMMARY                 	phosphoinositide (PIP) metabolisms, we measured PI in E11.5-E13.5 cortices by
ST:STUDY_SUMMARY                 	using liquid chromatography-mass spectrometry (LC-MS). In Mboat7 KO mice, PI
ST:STUDY_SUMMARY                 	with arachidonic acid (38:4 PI), which is the major species in Mboat7
ST:STUDY_SUMMARY                 	heterozygous mice, significantly decreased, and instead, PI with monounsaturated
ST:STUDY_SUMMARY                 	fatty acid (34:1, 34:2, 36:1, 36:2 PI) and PI with docosapentaenoic acid (DPA)
ST:STUDY_SUMMARY                 	or docosahexaenoic acid (DHA) (38:6, 40:5, 40:6, 40:7 PI) increased. On the
ST:STUDY_SUMMARY                 	other hand, the composition of molecular species of phosphatidylserine (PS),
ST:STUDY_SUMMARY                 	phosphatidylcholine (PC), and phosphatidylethanolamine (PE) showed little or no
ST:STUDY_SUMMARY                 	change in the cortices of E11.5-E13.5 Mboat7 KO mice compared with Mboat7
ST:STUDY_SUMMARY                 	heterozygous mice.
ST:INSTITUTE                     	University of Tokyo
ST:LAST_NAME                     	Kono
ST:FIRST_NAME                    	Nozomu
ST:ADDRESS                       	Hongo 7-3-1, Bunkyo-ku, Tokyo 113-033 JAPAN
ST:EMAIL                         	nozomu@mol.f.u-tokyo.ac.jp
ST:PHONE                         	81-3-5841-4723
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_1_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_2_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_3_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_4_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_1_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_2_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_3_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_4_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_5_PC_PE	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PC,PE KO 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_1_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_2_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_3_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_4_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_1_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_2_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_3_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_4_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_5_PS_PI	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PS PI KO 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_1_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_2_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_3_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_4_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_1_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_2_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_3_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_4_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_5_PC_PE	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PC,PE KO 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_1_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_2_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_3_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_4_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_1_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_2_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_3_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_4_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_5_PS_PI	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PI,PS KO 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_1_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_2_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_3_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_4_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_5_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE Het 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_1_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_2_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_3_PC_PE	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PC,PE KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_1_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_2_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_3_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_4_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_5_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS Het 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_1_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_2_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_3_PS_PI	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PI,PS KO 3.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Embryos at different gestational ages were collected from Mboat7 heterozygous
CO:COLLECTION_SUMMARY            	intercrosses, and the cortices were subsequently dissected from the embryos.
CO:SAMPLE_TYPE                   	Brain cortex
#TREATMENT
TR:TREATMENT_SUMMARY             	Not applicable.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Lipid extractions were conducted by the method of Bligh and Dyer. The extracted
SP:SAMPLEPREP_SUMMARY            	solutions were dried up with a centrifugal evaporator, dissolved in
SP:SAMPLEPREP_SUMMARY            	chloroform/methanol (2/1, v/v), and stored at -20 ºC. The phospholipid content
SP:SAMPLEPREP_SUMMARY            	of samples was quantified using a phosphorus assay. An aliquot of the extracted
SP:SAMPLEPREP_SUMMARY            	lipids was dried under a stream of nitrogen and dissolved in
SP:SAMPLEPREP_SUMMARY            	isopropanol:methanol (1:1, v/v) containing 0.25 µM internal standards
SP:SAMPLEPREP_SUMMARY            	(12:0/13:0 PC, PE, PS, PI) to achieve a final phospholipid concentration of 300
SP:SAMPLEPREP_SUMMARY            	µM.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	HILIC
CH:INSTRUMENT_NAME               	Shimadzu Nexera X2
CH:COLUMN_NAME                   	Merck SeQuant ZIC-HILIC (250 x 2.1mm, 1.8um)
CH:SOLVENT_A                     	95% water/5% acetonitrile; 10 mM ammonium acetate
CH:SOLVENT_B                     	50% water/50% acetonitrile; 20 mM ammonium acetate
CH:FLOW_GRADIENT                 	0–22 min: 0% B to 40% B; 22–25 min: 40% B to 40% B; 25–30 min: 0% B
CH:FLOW_RATE                     	0.3 mL/min
CH:COLUMN_TEMPERATURE            	50
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 4000 QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	The instrument parameters for positive ion mode were as follows: curtain gas, 10
MS:MS_COMMENTS                   	psi; collision gas, 7 arb. unit; ionspray voltage, 4500 V; temperature, 700°C;
MS:MS_COMMENTS                   	ion source gas 1, 30 psi; ion source gas 2, 50 psi. The specific detection of PC
MS:MS_COMMENTS                   	and PE species was performed using multiple reaction monitoring. 12:0/13:0 PC
MS:MS_COMMENTS                   	and PE were used as internal standards. Analyst (SCIEX) was used for data
MS:MS_COMMENTS                   	acquisition. MultiQuant (SCIEX) was used for data quantification. Gaussian
MS:MS_COMMENTS                   	smoothing width was 2.0 points. The peak area of the lipid species was divided
MS:MS_COMMENTS                   	by the corresponding internal standard area, and the total amount of
MS:MS_COMMENTS                   	phospholipids per cortex was calculated from the ratio of injection volume to
MS:MS_COMMENTS                   	total sample volume. The amounts of phospholipid per cortex are shown because
MS:MS_COMMENTS                   	the size of the E11-E13 cortices is not different between genotypes.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	pmol/cortex
MS_METABOLITE_DATA_START
Samples	E11.5_Het_1_PC_PE	E11.5_Het_2_PC_PE	E11.5_Het_3_PC_PE	E11.5_Het_4_PC_PE	E11.5_KO_1_PC_PE	E11.5_KO_2_PC_PE	E11.5_KO_3_PC_PE	E11.5_KO_4_PC_PE	E11.5_KO_5_PC_PE	E12.5_Het_1_PC_PE	E12.5_Het_2_PC_PE	E12.5_Het_3_PC_PE	E12.5_Het_4_PC_PE	E12.5_KO_1_PC_PE	E12.5_KO_2_PC_PE	E12.5_KO_3_PC_PE	E12.5_KO_4_PC_PE	E12.5_KO_5_PC_PE	E13.5_Het_1_PC_PE	E13.5_Het_2_PC_PE	E13.5_Het_3_PC_PE	E13.5_Het_4_PC_PE	E13.5_Het_5_PC_PE	E13.5_KO_1_PC_PE	E13.5_KO_2_PC_PE	E13.5_KO_3_PC_PE
Factors	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-
PC 30:0	470.9686414	494.1286929	453.3516158	740.0447762	422.5852878	377.8502107	340.3860273	375.6009965	586.2053112	699.2834195	1984.449942	1233.841704	2136.915737	1825.499086	1569.455048	2036.505187	1910.91394	2224.868538	3461.912223	2850.233257	2724.604831	3550.551842	4486.135648	3626.836198	2850.294233	3436.674197
PC 32:0	1105.147442	1012.171914	1111.06235	1748.436223	948.7219279	1006.862161	975.6579691	1113.121426	1419.96193	1360.599822	2416.726272	1330.526808	1972.997161	1784.221211	1658.827192	2126.63846	1751.431408	2168.481573	2993.702783	2511.558482	2721.138412	3772.973037	4684.723655	4047.788916	3246.606901	3699.834162
PC 32:1	675.8173473	699.6048359	673.7113796	868.1046983	634.9752967	609.7080452	518.8919011	657.9548283	889.086005	1186.871957	2150.524477	1149.038385	1957.314746	1544.630064	1509.873619	1877.233541	1429.589907	1824.118322	2875.468076	2524.971344	2722.62402	3460.491726	4245.225606	3496.145903	3248.86188	3312.566659
PC 34:0	581.4294497	535.9791172	481.4790659	971.710967	457.1024052	364.9281976	510.0672089	473.3201288	692.5712872	961.3708874	2032.301927	1148.572432	1853.512095	1496.771658	1443.990308	1878.681465	1449.724976	1615.083787	2740.68051	2283.539821	2556.235939	3036.799817	3680.389223	3201.551205	2552.636924	2838.878724
PC 34:1	1353.792132	1301.66357	1071.76252	1796.056496	1006.08419	891.8826163	1015.898565	1086.555282	1431.409389	1057.323894	2045.973922	1074.951968	1732.160074	1398.96104	1218.554805	1760.675654	1531.543669	1699.26147	2981.879312	2224.140002	2357.659687	3508.250878	4343.705724	3338.739912	2785.46357	3708.931049
PC 34:2	429.4919024	535.9791172	474.1243834	698.2161584	365.422547	335.286682	441.9405851	437.0130656	587.1592662	768.0842561	1582.734544	1087.53268	1966.276126	1560.782276	1456.880521	1756.693863	1598.660567	2018.653351	2962.370585	2428.686154	2477.003519	3134.364943	3665.739288	3153.174134	2924.708561	2938.944488
PC 36:1	409.6434759	485.0034125	430.0524306	772.8641532	330.8555493	312.6599734	404.5238902	458.7087497	536.5996543	980.6995505	2037.529454	1205.884566	1863.593647	1436.051305	1327.691942	1823.29837	1414.568506	1484.185475	2906.800273	2398.507213	2695.883079	3217.602322	4150.001028	3496.867949	2514.866016	2786.896508
PC 36:2	532.0241272	585.8010508	573.2160988	837.8593901	473.1139583	426.7428887	462.0608833	510.5127302	601.9455678	809.9630263	1935.793723	1211.70897	1926.323307	1548.817675	1567.449904	1956.145402	1735.770799	1885.741219	3054.593657	2425.332938	2598.328162	3182.124095	4047.451483	3228.988946	2723.45163	2764.154289
PC 36:3	328.1462682	224.3560336	363.4111467	518.8679157	301.5758558	294.8856125	277.5895177	288.2869372	389.8336866	675.3526938	1312.511573	851.2948617	1194.477275	1000.240692	935.2565672	1168.474715	949.5442909	1001.674159	1586.709769	1399.248965	1539.089752	1521.47014	2062.385292	2010.897633	1356.370238	1464.598911
PC 36:4	328.9013714	288.285441	355.495038	594.6098897	228.1022806	339.1896573	343.8453067	396.1012042	455.4657866	485.0574032	1500.300453	913.0335421	1398.72206	1113.904407	1128.896212	1441.4084	1128.203079	1537.350328	2212.171369	1749.899511	1817.393626	2381.817156	2703.726891	2080.214033	1877.834274	1956.480622
PC 38:4	328.9013714	298.9316015	285.934338	541.519721	282.122567	213.4505587	219.7701344	354.3480815	412.776303	608.6227854	1391.728724	890.4348553	1399.468842	1092.069009	1089.939123	1288.652412	1045.105968	1219.166739	1835.593827	1653.61432	1764.902148	2346.338929	2679.310332	2430.40626	1633.168986	1975.973952
PC 38:5	282.8400774	268.3566676	224.2336042	479.7420701	291.8492114	221.6257099	246.6324975	325.5238154	342.3744283	523.0244201	1251.38971	696.3657202	1120.919281	882.0902515	818.3853025	1073.273708	877.9529341	987.5774176	1523.454201	1201.888276	1396.471396	1817.576884	2174.701461	1935.082821	1382.302504	1677.725993
PC 38:6	174.6985146	240.7186055	154.0553355	317.8331434	211.3425241	104.7474202	201.661866	229.1329903	257.6632296	433.0540953	832.3832585	419.1240986	667.6228093	533.9203455	509.0201899	620.4354588	496.3454338	541.7176292	1011.497919	841.1780839	958.7122778	1256.747981	1442.204711	1367.5548	839.9799066	1152.055843
PE 34:0	109.275882	410.5911242	63.67010061	446.4467403	102.2863397	162.4701189	136.6799205	68.91237025	170.8875355	337.4086551	458.4617786	332.3827905	311.2429395	478.8746703	367.034289	622.1023736	318.6282906	449.1184814	613.1609794	543.4276993	694.2953762	769.7137244	1111.744845	934.5116843	753.2555311	776.1006737
PE 34:1	533.5896299	1317.65633	325.5510285	1982.107189	594.8653299	861.1501278	605.3263751	327.6322289	627.9177987	1400.596495	3053.322492	2029.747065	2800.816589	2674.313598	2138.532787	4041.698946	2353.533961	2688.170875	4412.417117	3548.521006	4527.834844	4565.670828	6318.345545	5278.362986	5713.2018	5242.014662
PE 34:2	221.7747887	413.8264411	172.0852845	853.629435	328.7094468	257.2058694	280.5044732	168.3176325	338.1366688	492.9164792	1063.771378	858.8777072	876.8617931	942.9112136	681.3005376	1169.47843	851.119682	779.8401751	1277.950965	1131.711385	1216.864167	1152.228293	1659.808077	1862.821348	1568.922542	1275.386876
PE 36:1	341.4871313	847.6530229	282.4933411	1074.307913	215.5585407	284.1764633	448.3515573	288.3907344	397.2900464	878.4367924	1718.716776	1249.407671	1413.815967	1441.541297	1216.372858	1888.979502	937.6007899	1135.481574	2201.773077	1947.626313	2487.640903	2210.76484	3369.10817	3027.915318	2716.273202	2738.717241
PE 36:2	451.479241	1431.77478	326.1328891	1985.015637	261.4773617	791.2605751	511.5142478	434.1029174	573.2243979	1362.403591	2491.058047	2043.437229	2465.162438	2280.068012	1695.017773	2777.366677	2111.768138	1584.261778	3415.320849	2784.164683	3137.602085	2149.504853	4156.112367	3561.732137	2861.507463	2770.10655
PE 36:3	207.4502348	395.296899	151.8656272	876.5334615	49.59232665	407.3298602	250.9940358	188.7701821	275.6969923	400.0564185	702.0067261	696.9734951	687.2125747	673.7544903	607.8936798	708.1648812	500.6892204	511.5868818	950.2575826	779.5661113	941.8279611	678.7246254	970.1652245	1258.124287	750.3115934	857.3205117
PE 36:4	150.6636108	663.8282001	310.2771866	1144.474217	225.6086978	309.4229097	335.4870775	230.2624357	326.6346231	714.2643095	1227.507727	761.6054836	1092.95649	1197.402346	1106.591914	1286.310598	985.2607192	920.1121788	1687.789467	1005.263906	1525.698353	1319.432023	1851.08255	2041.793957	1437.819182	1874.137952
PE 38:3	133.5253053	586.7688344	255.2913561	1017.229625	128.4687316	324.2013173	272.9456594	180.4521595	403.7452762	691.4625758	1078.188415	977.7659804	1154.909184	919.6188049	657.2951046	1081.680791	699.3877981	789.8757123	1834.515199	1376.61476	1542.118425	1506.094809	2042.55986	1500.224612	1612.296558	1411.145639
PE 38:4	492.5344355	1803.247982	664.9212472	2827.738392	511.5183378	1249.083328	791.9151756	521.8825199	885.0706766	1511.184904	3596.020959	2949.870246	3589.557609	3242.820908	2564.482849	4340.141513	2649.025522	3418.848194	5863.706704	5231.909467	4635.933651	6516.981604	7419.745748	6839.943307	6229.372214	5977.7016
PE 38:5	329.3368401	1306.7739	411.2300078	1822.142559	323.1645325	809.1178176	590.0016567	388.5005821	520.8783534	1246.114749	2476.64101	1705.50632	2519.717796	2000.559107	1869.935411	2809.755793	1586.642372	1699.275801	3472.095013	2714.560566	2954.244614	3288.219913	4239.275181	4115.484023	3316.836497	2915.282106
PE 38:6	156.6748074	576.768764	327.2966104	588.5971274	251.2539261	377.4651615	361.7876077	165.6754371	371.9385989	894.9680494	1289.295029	983.5302603	1582.105376	1269.004936	1164.409878	1781.401367	974.8621892	1384.678622	2851.127586	2206.708305	3077.395154	3848.568622	3732.184849	4286.039605	3140.200234	3733.365981
PE 40:4	114.6475897	444.1207719	178.3402864	805.27649	141.2740179	298.3083156	227.6963221	139.2534832	269.5938661	490.0092581	925.7797371	805.558118	850.8786481	890.2876235	726.3107246	1129.339062	690.9823197	755.822541	1029.209155	1009.001905	1237.799758	1443.213233	1287.806609	2005.467834	1627.212509	1802.334906
PE 40:5	140.6875822	674.1223902	278.8567121	713.6603837	169.1025562	431.0368891	337.2473492	224.1951722	254.1013148	718.8246562	1135.856563	881.2142918	1204.841207	1281.945163	859.950727	1347.618567	983.5276309	1083.386987	1568.918559	1297.987887	1721.370878	2216.170133	2287.991581	2268.167729	2194.214913	2407.167661
PE 40:6	276.7708435	972.6539024	419.3760568	1272.809477	198.7505195	559.7321889	391.9193174	285.3571026	301.4005434	816.302068	1818.606247	1341.63615	2027.794909	1828.885428	1537.665087	2122.643837	1380.40486	1774.824227	3482.740169	2456.76754	3518.000418	4306.216763	4352.863416	3922.778367	4268.709693	4580.877334
PE 40:7	239.5525831	767.0642206	231.580535	971.7851276	90.38210197	360.2236859	297.4859178	226.7395085	363.1360129	637.8785015	1361.380214	904.9919465	1395.322626	1138.739984	992.4197326	1407.769782	916.8037299	966.3433054	2001.289308	1620.22917	2101.769211	2248.601892	2590.217419	2209.470029	2137.298784	2377.740183
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
PC 30:0
PC 32:0
PC 32:1
PC 34:0
PC 34:1
PC 34:2
PC 36:1
PC 36:2
PC 36:3
PC 36:4
PC 38:4
PC 38:5
PC 38:6
PE 34:0
PE 34:1
PE 34:2
PE 36:1
PE 36:2
PE 36:3
PE 36:4
PE 38:3
PE 38:4
PE 38:5
PE 38:6
PE 40:4
PE 40:5
PE 40:6
PE 40:7
METABOLITES_END
#END