#METABOLOMICS WORKBENCH nozomu_kono_20250125_013228 DATATRACK_ID:5569 STUDY_ID:ST003748 ANALYSIS_ID:AN006155 PROJECT_ID:PR002333
VERSION             	1
CREATED_ON             	February 24, 2025, 5:14 pm
#PROJECT
PR:PROJECT_TITLE                 	Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures
PR:PROJECT_TITLE                 	embryonic radial glial cell integrity
PR:PROJECT_SUMMARY               	Arachidonic acid, a vital polyunsaturated fatty acid in brain development, is
PR:PROJECT_SUMMARY               	enriched in phosphatidylinositol (PI). The arachidonic acyl chain in PI is
PR:PROJECT_SUMMARY               	introduced by lysophospholipid acyltransferase 11 (LPLAT11)/membrane-bound
PR:PROJECT_SUMMARY               	O-acyltransferase 7 (MBOAT7), the loss of which causes microcephaly in humans
PR:PROJECT_SUMMARY               	and mice. Here, we show that LPLAT11 deficiency impaired indirect neurogenesis
PR:PROJECT_SUMMARY               	in the developing neocortex, resulting in fewer layer II-V neurons.
PR:PROJECT_SUMMARY               	LPLAT11-deficient radial glial cells had defects in differentiation into
PR:PROJECT_SUMMARY               	intermediate progenitor cells and increased apoptosis. Prior to these anomalies,
PR:PROJECT_SUMMARY               	LPLAT11 deficiency caused a rounding of the Golgi apparatus, accompanied by
PR:PROJECT_SUMMARY               	impaired apical trafficking of E-cadherin, and deregulated apical detachment.
PR:PROJECT_SUMMARY               	Moreover, impaired PI acyl chain remodeling led to a decreased amount of
PR:PROJECT_SUMMARY               	PI(4,5)P2, leading to Golgi apparatus rounding. Thus, these results clarify the
PR:PROJECT_SUMMARY               	underlying mechanism of microcephaly by LPLAT12 deficiency and highlight the
PR:PROJECT_SUMMARY               	critical role of arachidonic acid in PI in the integrity of radial glial cells.
PR:INSTITUTE                     	University of Tokyo
PR:DEPARTMENT                    	Grad School of Phaemceutical Sciences
PR:LABORATORY                    	Department of Health Chemistry
PR:LAST_NAME                     	Kono
PR:FIRST_NAME                    	Nozomu
PR:ADDRESS                       	Hongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan
PR:EMAIL                         	nozomu@mol.f.u-tokyo.ac.jp
PR:PHONE                         	81-3-5841-4723
#STUDY
ST:STUDY_TITLE                   	Phosphatidylinositol remodeling by lysophospholipid acyltransferase 11 ensures
ST:STUDY_TITLE                   	embryonic radial glial cell integrity - part 2
ST:STUDY_SUMMARY                 	Since fragmentation of the Golgi apparatus is associated with defects in
ST:STUDY_SUMMARY                 	phosphoinositide (PIP) metabolisms, we measured PIPs in E11.5-E13.5 cortices by
ST:STUDY_SUMMARY                 	supercritical fluid chromatography-mass spectrometry (SFC-MS). In Mboat7 KO
ST:STUDY_SUMMARY                 	mice, PI4P with arachidonic acid (38:4 PI4P), which is the major species in
ST:STUDY_SUMMARY                 	Mboat7 heterozygous mice, significantly decreased, and instead, PI4P with
ST:STUDY_SUMMARY                 	monounsaturated fatty acid (34:1, 34:2, 36:1, 36:2 PI) and PI4P with
ST:STUDY_SUMMARY                 	docosapentaenoic acid (DPA) or docosahexaenoic acid (DHA) (38:6, 40:5, 40:6,
ST:STUDY_SUMMARY                 	40:7 PI4P) increased. PI(4,5)P2 also showed changes in the molecular species
ST:STUDY_SUMMARY                 	similar to those observed in PI4P. Interestingly, the total amount of PI(4,5)P2
ST:STUDY_SUMMARY                 	started to decrease at E12.5 in Mboat7 KO mice, whereas the total amount of PI4P
ST:STUDY_SUMMARY                 	was unchanged. This is because the decrease of arachidonic acid-containing
ST:STUDY_SUMMARY                 	PI(4,5)P2 species was not fully compensated by the increase of other PI(4,5)P2
ST:STUDY_SUMMARY                 	species. To rule out the possibility that changes in systemic lipid metabolism
ST:STUDY_SUMMARY                 	in global Mboat7 KO mice affect radial glial cells in a non-cell-autonomous
ST:STUDY_SUMMARY                 	manner, we generated telencephalon-specific Mboat7 KO mice (hereafter,
ST:STUDY_SUMMARY                 	Mboat7-Emx1-KO) by crossing Mboat7fl/fl mice with Emx1-Cre transgenic mice, in
ST:STUDY_SUMMARY                 	which Cre expression starts at E10.5 in the dorsal telencephalon. The number of
ST:STUDY_SUMMARY                 	cleaved caspase-3-positive cells increased in E13.5 and E14.5 cortices of
ST:STUDY_SUMMARY                 	Mboat7-Emx1-KO mice but not as much as in Mboat7 KO mice. Moreover, whereas
ST:STUDY_SUMMARY                 	p-H3-positive RGCs were located at the ventricular wall at E12.5, some
ST:STUDY_SUMMARY                 	p-H3-positive RGCs were located apart from the ventricular zone from E13.5
ST:STUDY_SUMMARY                 	cortices of Mboat7-Emx1-KO mice (Fig 8C and D). Taken together, these results
ST:STUDY_SUMMARY                 	indicate that telencephalon-specific LPLAT11 deficiency caused the apical
ST:STUDY_SUMMARY                 	detachment and apoptosis of RGCs. In Mboat7-Emx1-KO mice, the change in the
ST:STUDY_SUMMARY                 	fatty acyl composition of PI and PIPs was delayed compared to Mboat7 KO mice,
ST:STUDY_SUMMARY                 	probably because Emx1-Cre drives the expression of Cre recombinase only from
ST:STUDY_SUMMARY                 	E10.5. Therefore, we generated neural-specific Mboat7 KO mice (hereafter,
ST:STUDY_SUMMARY                 	Mboat7-Sox1-KO) by crossing Mboat7fl/fl mice with Sox1-Cre transgenic mice,
ST:STUDY_SUMMARY                 	which drives the expression of Cre recombinase before E8.5 in the
ST:STUDY_SUMMARY                 	neuroepithelium. Detachment of RGCs from the ventricular wall was not observed
ST:STUDY_SUMMARY                 	in the E12.5 cortices of Mboat7-Sox1-KO. On the other hand, E13.5 cortices of
ST:STUDY_SUMMARY                 	Mboat7-Sox1-KO showed detachment from the ventricular wall and apoptosis to the
ST:STUDY_SUMMARY                 	same extent as those of Mboat7 KO mice. Also, Mboat7-Sox1-KO showed rounding of
ST:STUDY_SUMMARY                 	the Golgi apparatus at E12.5 when the detachment of RGCs from the ventricular
ST:STUDY_SUMMARY                 	wall was not observed, which is consistent with the phenotype observed in E12.0
ST:STUDY_SUMMARY                 	cortices of Mboat7 KO mice. Moreover, E-cadherin overexpression failed to rescue
ST:STUDY_SUMMARY                 	the detachment of RGCs from the apical ventricular wall. In the Mboat7-Sox1-KO
ST:STUDY_SUMMARY                 	cortices, a significant change in the fatty acyl composition of PI and PIPs was
ST:STUDY_SUMMARY                 	observed. The total amount of PI(4,5)P2 started to decrease at E12.5 in the
ST:STUDY_SUMMARY                 	Mboat7-Sox1-KO cortices as well as in the Mboat7 KO cortices. Taken together,
ST:STUDY_SUMMARY                 	these results indicate that Mboat7-Sox1-KO mice mimicked almost all the
ST:STUDY_SUMMARY                 	phenotypes observed in the brain of Mboat7 KO mice.
ST:INSTITUTE                     	University of Tokyo
ST:LAST_NAME                     	Kono
ST:FIRST_NAME                    	Nozomu
ST:ADDRESS                       	Hongo 7-3-1,, Bunyo-ku, Tokyo, 113-0033, Japan
ST:EMAIL                         	nozomu@mol.f.u-tokyo.ac.jp
ST:PHONE                         	81-3-5841-4723
#SUBJECT
SU:SUBJECT_TYPE                  	Mammal
SU:SUBJECT_SPECIES               	Mus musculus
SU:TAXONOMY_ID                   	10090
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_1	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PIPs Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_2	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PIPs Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_Het_3	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PIPs Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_1	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PIPs KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_2	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PIPs KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E11.5_KO_3	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E11.5 cortex PIPs KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_1	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_2	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Het_3	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_1	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_2	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_KO_3	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_1	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs Het 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_2	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs Het 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_3	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs Het 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_4	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs Het 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_5	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs Het 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_6	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs Het 6.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Het_7	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs Het 7.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_1	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs KO 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_2	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs KO 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_3	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs KO 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_4	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs KO 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_5	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs KO 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_KO_6	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs KO 6.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_flox_1	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_flox_2	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_flox_3	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Emx1_KO1	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Emx1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl Emx1-Cre 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Emx1_KO2	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Emx1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl Emx1-Cre 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Emx1_KO3	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Emx1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl Emx1-Cre 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Sox1-Het_1	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs + fl Sox1-Cre 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Sox1-Het_2	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs + fl Sox1-Cre 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Sox1-Het_3	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs + fl Sox1-Cre 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Sox1-Het_4	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs + fl Sox1-Cre 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Sox1-Het_5	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs + fl Sox1-Cre 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Sox1_KO1	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl Sox1-Cre 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Sox1_KO2	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl Sox1-Cre 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Sox1_KO3	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl Sox1-Cre 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E12.5_Sox1_KO4	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E12.5 cortex PIPs fl fl Sox1-Cre 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_flox_1	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_flox_2	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_flox_3	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Emx1_KO1	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Emx1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl Emx1-Cre 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Emx1_KO2	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Emx1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl Emx1-Cre 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Emx1_KO3	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Emx1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl Emx1-Cre 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Sox1-Het_1	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs + fl Sox1-Cre 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Sox1-Het_2	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs + fl Sox1-Cre 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Sox1-Het_3	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs + fl Sox1-Cre 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Sox1_KO1	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl Sox1-Cre 1.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Sox1_KO2	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl Sox1-Cre 2.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Sox1_KO3	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl Sox1-Cre 3.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Sox1_KO4	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl Sox1-Cre 4.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Sox1_KO5	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl Sox1-Cre 5.mzML
SUBJECT_SAMPLE_FACTORS           	-	E13.5_Sox1_KO6	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	RAW_FILE_NAME(Raw file name)=E13.5 cortex PIPs fl fl Sox1-Cre 6.mzML
#COLLECTION
CO:COLLECTION_SUMMARY            	Embryos at different gestational ages were collected from Mboat7 heterozygous
CO:COLLECTION_SUMMARY            	intercrosses or from crosses between Mboat7 f/f; Sox1-Cre and Mboat7 +/f;
CO:COLLECTION_SUMMARY            	Sox1-Cre, or between Mboat7 f/f; Emx1-Cre and Mboat7 f/f, and the cortices were
CO:COLLECTION_SUMMARY            	subsequently dissected from the embryos.
CO:SAMPLE_TYPE                   	Brain cortex
#TREATMENT
TR:TREATMENT_SUMMARY             	Not applicable.
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	"Cortices from E11.5 to E13.5 were isolated in ice-cold DMEM and immediately
SP:SAMPLEPREP_SUMMARY            	collected into a safe-lock poly-propylene tube (2ml) with 750 µl of ice-cold
SP:SAMPLEPREP_SUMMARY            	quench mix and sonicated. 170 µl of H2O was mixed and stored at -80 ºC before
SP:SAMPLEPREP_SUMMARY            	lipid extraction. The single-phase sample (a mixture of 170 µl of an aqueous
SP:SAMPLEPREP_SUMMARY            	sample, 10 ng of internal standards [17:0/20:4 PI, 17:0/20:4 PI4P, and 17:0/20:4
SP:SAMPLEPREP_SUMMARY            	PI(4,5)P2], and 750 µl of quench mix) were mixed with 725 µl of CHCl3 and 170
SP:SAMPLEPREP_SUMMARY            	µl of 2 M HCl, followed by vortex-mixing and centrifugation (1,500 g, 5 min at
SP:SAMPLEPREP_SUMMARY            	room temperature). The lower organic phase was collected into a new 2 ml
SP:SAMPLEPREP_SUMMARY            	safe-lock poly-propylene tube and mixed with 708 µl of pre-derivatization wash
SP:SAMPLEPREP_SUMMARY            	solution, followed by vortex-mixing and centrifugation (1,500 g, 3 min at room
SP:SAMPLEPREP_SUMMARY            	temperature). The lower phase was collected into another fresh tube and
SP:SAMPLEPREP_SUMMARY            	subjected to derivatization. Fifty µl trimethylsilyl diazomethane in hexane (2
SP:SAMPLEPREP_SUMMARY            	M solution) was added to the lipid extracts (~1 ml), and after leaving to stand
SP:SAMPLEPREP_SUMMARY            	10 min at room temperature, the reaction was quenched with 6 µl of acetic acid.
SP:SAMPLEPREP_SUMMARY            	Next, 700 µl post-derivatization wash solution was added to the mixture,
SP:SAMPLEPREP_SUMMARY            	followed by centrifugation (1,500 g, 3 min). The resultant lower phase was
SP:SAMPLEPREP_SUMMARY            	collected and rewashed with a 700 µl post-derivatization wash solution. Samples
SP:SAMPLEPREP_SUMMARY            	were temporarily stored at -80 ºC. Ninety µl of methanol and 10 µl of H2O
SP:SAMPLEPREP_SUMMARY            	were added to the final collected lower phase. The samples were dried up with a
SP:SAMPLEPREP_SUMMARY            	centrifugal evaporator. Samples were dissolved in 80 µl methanol, sonicated for
SP:SAMPLEPREP_SUMMARY            	1 min, and 20 µl H2O was added. After centrifugation at 15,000 g for 30 min at
SP:SAMPLEPREP_SUMMARY            	4 ºC, the supernatants were collected. To avoid degradation, the samples were
SP:SAMPLEPREP_SUMMARY            	stored at -80 ºC until use. "
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_TYPE           	Supercritical fluid chromatography
CH:INSTRUMENT_NAME               	Shimadzu Nexera UC/s
CH:COLUMN_NAME                   	ULTRON AF-HILIC-CD (250 x 2.0 mm, 5.0um)
CH:SOLVENT_A                     	99.99% supercritical carbon dioxide
CH:SOLVENT_B                     	2.5% water/97.5% methanol; 0.1% formic acid
CH:FLOW_GRADIENT                 	0-16 min: 5% B to 20% B; 16.01-18 min: 40% B; 18.01-22 min: 5% B
CH:FLOW_RATE                     	1.5 mL/min
CH:COLUMN_TEMPERATURE            	10ºC
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	ABI Sciex 4500 QTrap
MS:INSTRUMENT_TYPE               	Triple quadrupole
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	POSITIVE
MS:MS_COMMENTS                   	The instrument parameters of QTRAP4500 for positive ion mode were as follows:
MS:MS_COMMENTS                   	curtain gas, 20 psi; ionspray voltage, 4500 V; temperature, 300 ºC; ion source
MS:MS_COMMENTS                   	gas 1, 18 psi; ion source gas 2, 20 psi. The specific detection of
MS:MS_COMMENTS                   	phosphoinositide species was performed using mlutiple reaction monitoring.
MS:MS_COMMENTS                   	17:0/20:4 PI4P and 17:0/20:4 PI4,5P2 were used as internal standards. Analyst
MS:MS_COMMENTS                   	(SCIEX) was used for data acquisition. MultiQuant (SCIEX) was used for data
MS:MS_COMMENTS                   	quantification. Gaussian smoothing width was 2.0 points. Retention time of PI4P
MS:MS_COMMENTS                   	and PI(4,5)P2 is confirmed by measuring standards [17:0/20:4 PI3P, PI4P, PI5P,
MS:MS_COMMENTS                   	PI(3,4)P2, PI(3,5)P2, and PI(4,5)P2] before measuring samples. Peak areas of
MS:MS_COMMENTS                   	PI4P and PI(4,5)P2 were integrated from the ion chromatogram of PIP and of PIP2,
MS:MS_COMMENTS                   	respectively, based on retention time, and isotopic correction was applied. The
MS:MS_COMMENTS                   	peak area of the lipid species was divided by the corresponding internal
MS:MS_COMMENTS                   	standard area, and the total amount of phosphoinositides per cortex was
MS:MS_COMMENTS                   	calculated from the ratio of injection volume to total sample volume. The
MS:MS_COMMENTS                   	amounts of phospholipid per cortex are shown because the size of the E11-E13
MS:MS_COMMENTS                   	cortices is not different between genotypes.
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	pmol/cortex
MS_METABOLITE_DATA_START
Samples	E11.5_Het_1	E11.5_Het_2	E11.5_Het_3	E11.5_KO_1	E11.5_KO_2	E11.5_KO_3	E12.5_Het_1	E12.5_Het_2	E12.5_Het_3	E12.5_KO_1	E12.5_KO_2	E12.5_KO_3	E13.5_Het_1	E13.5_Het_2	E13.5_Het_3	E13.5_Het_4	E13.5_Het_5	E13.5_Het_6	E13.5_Het_7	E13.5_KO_1	E13.5_KO_2	E13.5_KO_3	E13.5_KO_4	E13.5_KO_5	E13.5_KO_6	E12.5_flox_1	E12.5_flox_2	E12.5_flox_3	E12.5_Emx1_KO1	E12.5_Emx1_KO2	E12.5_Emx1_KO3	E12.5_Sox1-Het_1	E12.5_Sox1-Het_2	E12.5_Sox1-Het_3	E12.5_Sox1-Het_4	E12.5_Sox1-Het_5	E12.5_Sox1_KO1	E12.5_Sox1_KO2	E12.5_Sox1_KO3	E12.5_Sox1_KO4	E13.5_flox_1	E13.5_flox_2	E13.5_flox_3	E13.5_Emx1_KO1	E13.5_Emx1_KO2	E13.5_Emx1_KO3	E13.5_Sox1-Het_1	E13.5_Sox1-Het_2	E13.5_Sox1-Het_3	E13.5_Sox1_KO1	E13.5_Sox1_KO2	E13.5_Sox1_KO3	E13.5_Sox1_KO4	E13.5_Sox1_KO5	E13.5_Sox1_KO6
Factors	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E11.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7-/-	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Emx1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Emx1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Emx1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7+/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E12.5 | Genotype:Mboat7f/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Emx1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Emx1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Emx1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7+/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre	Sample source:brain cortex | Gestational ages:E13.5 | Genotype:Mboat7f/f  Sox1-Cre
PI4P 32:1	0.490298725	0.793707939	0.480099366	2.342483392	2.391283639	3.703670491	2.117885159	2.659923279	2.78971246	19.74255343	30.27710864	25.71539285	3.526803797	3.099919564	4.803404369	4.829598974	3.272068553	4.018801854	3.952716945	51.22438297	50.85848632	65.17829442	56.41823453	45.77894856	66.53275416	2.183694508	3.360287684	1.703810023	5.658062825	4.434223788	4.436574392	2.041126337	2.412174616	2.08112631	2.145309466	1.283801859	18.09085358	15.29772644	13.6458645	23.11537342	3.27736771	5.678514973	4.236168273	16.66411952	20.05949632	15.47522882	1.543200556	2.619611682	1.953318752	26.47334048	35.54028528	38.98627655	20.59599946	35.90407467	20.25485629
PI4P 34:0	0.148711679	0.307673302	0.361291765	0.982752656	1.09897858	1.371963231	1.43666898	1.28931984	1.894457172	10.03204669	11.56435949	12.00671623	1.099314874	1.27803661	1.093365265	1.37705626	1.209303473	1.793288634	1.300099857	10.31423165	9.576596129	9.878283566	10.75106104	9.016639468	11.90389927	1.428238836	1.786838814	1.233073051	3.408643654	2.458015137	2.094095795	0.949435203	2.328212506	1.450848932	1.799969897	1.081352174	7.435330121	7.807865845	5.176753183	7.238663116	1.420392575	2.941530283	1.709398805	5.035053088	7.667826652	6.160117158	1.046981245	1.253225351	1.238845531	11.44142394	11.96553321	11.81361624	9.188732298	8.78774847	10.3849176
PI4P 34:1	2.499184274	2.057796861	2.502893314	13.22575528	12.20763736	17.78302518	10.85302699	11.24623073	11.63647917	80.16797558	111.0009705	86.63667987	9.919190597	9.038840994	11.56663992	15.01180594	9.733333888	11.18535276	10.88696768	84.09867588	89.04823294	110.8558458	89.8346317	91.65458587	130.2167958	10.73602287	14.90106005	9.010234585	29.73041846	18.91992772	15.81261567	7.486544295	13.09303887	10.92749891	9.993826854	9.580766374	66.86447951	71.88592455	52.22974194	62.10405232	9.880079235	18.9093852	15.19322582	42.76213401	56.13772712	46.0375568	8.412606947	7.732667057	7.072434321	100.1429909	87.5870402	61.19444259	59.6743278	65.01416871	40.72747509
PI4P 34:2	2.232941298	2.621873988	1.988192192	11.72967682	12.39254308	17.80465245	8.901290456	8.755083392	8.737590749	53.61673698	112.768212	79.41396159	9.033043851	8.011645676	11.91605712	12.85654901	8.717932985	10.09457281	10.06853042	87.71802124	94.95420901	127.3123623	103.1658673	80.68513186	140.5131564	8.774688896	10.82651999	6.494303271	25.0411531	16.99785785	16.43629617	5.325181275	11.70419193	7.098549426	8.378238258	6.33045926	57.77091029	55.86013346	40.31894306	59.85686622	8.692670623	16.35731479	12.20263605	39.76927029	42.27887606	35.05079763	7.588539036	6.400098949	7.15323784	64.79635332	52.90853848	57.21031282	52.82537969	65.4475965	41.02174875
PI4P 36:1	1.174273625	1.371990004	1.14586187	5.793731921	4.913685436	6.767139911	5.937298161	5.963966586	5.475519665	22.67622276	31.94856721	34.00236985	2.403324708	2.290664141	2.785392894	3.920917264	2.836645172	3.241867491	3.112735499	14.81442974	14.48963146	18.04870762	15.43612764	21.19355738	23.96888881	5.95812209	6.534563757	4.449553439	9.947431445	7.605209403	8.012524162	3.502798782	4.12171894	3.917753436	4.210139798	4.580293892	21.32174522	23.46962461	14.3071805	16.02311788	3.313349789	6.687311042	4.017773697	10.67232135	12.9593005	12.60076367	2.627531641	2.44921986	2.789422532	16.4764879	15.41681062	16.71543882	12.1380401	11.67546113	16.43224139
PI4P 36:2	4.219063465	6.175027443	5.396835904	22.54969701	21.40853921	20.9370588	16.48025808	20.89378404	18.0727349	75.93548897	120.21699	103.019256	10.84920952	9.679296257	11.34748316	16.60142189	11.67699274	13.30845659	13.51057366	58.21562907	55.21026331	72.32794164	59.21941411	67.68848058	90.72046879	18.82652549	20.74713927	15.52231829	34.77673449	25.70589423	29.71333739	12.7045266	18.74312074	15.42536126	17.41712613	16.32445912	62.15722015	79.9615843	51.98085958	58.86946626	11.82611002	24.30482934	17.26132378	38.3487224	49.01828957	46.72927302	12.41437558	9.846306075	7.161743474	62.21426924	42.88201826	40.61574086	36.30925601	56.39979135	42.02227921
PI4P 36:3	3.446778227	4.396437566	5.183268922	9.121939352	8.698674047	10.79910565	21.72576883	32.91274884	30.16474777	41.77185821	62.27807193	52.83422354	30.6911871	28.71914588	36.83004179	45.78782695	35.07140965	37.22158376	41.24372954	52.49132283	50.71816126	67.2071809	60.38785429	50.598391	74.56097164	22.26456149	31.19116785	17.70931159	37.25228574	24.20490375	27.80689045	18.3976611	25.73154602	24.24232477	28.78630536	17.92530483	25.13569514	31.57039003	33.54578728	36.97642589	37.93110845	71.6226211	46.38203083	44.95973374	69.79935051	59.71816634	36.63841503	33.09633528	27.22228027	47.00090895	40.99686674	42.09300246	40.79875789	41.59281443	32.95865036
PI4P 36:4	9.725041813	8.00951516	12.98505045	8.208251974	8.92003462	13.34791356	46.24084954	67.78182293	66.04897657	54.93556119	80.37984963	75.00508227	134.5099461	117.2340521	173.3150971	167.2383586	136.7404136	131.2603973	172.9308775	156.5084992	154.7885417	183.0895508	198.0747548	126.1908177	202.8900978	44.46640591	83.54417619	45.82906689	87.59642909	49.81340547	56.13124507	47.48624651	60.49059715	44.55190322	57.19423866	49.41439126	46.76234239	44.89732075	81.06454161	61.28689374	151.1847026	258.1200739	150.7737801	115.3436323	172.3577346	156.3662927	103.3345589	92.47983645	136.8556443	142.3286619	140.0812597	158.8280046	176.0081354	151.0495853	113.97219
PI4P 38:2	1.353484147	1.963197621	1.49615522	1.096088958	1.52368282	1.45669821	11.47717206	14.62306935	16.59941749	7.314983009	8.992257406	8.96600911	9.385025456	8.866250237	11.61595763	14.43033089	13.87869608	14.2930154	17.25779215	6.728526819	7.8440643	7.55271519	7.634170893	10.62403371	11.04231337	10.41103439	13.88242504	6.063127762	11.96357752	7.863013112	10.24306709	6.820172318	8.97068864	12.85350664	13.18896861	5.972786576	9.425217031	6.828744405	7.306475132	9.022792689	10.17693139	22.81664932	15.4721187	13.81088229	23.79720103	17.21604795	12.34323303	10.47714603	10.30032226	10.55514102	7.04756648	7.735478917	5.741319182	8.798584165	6.020839164
PI4P 38:3	15.75256742	17.20697701	17.61496315	7.506437129	8.455349341	10.14915541	108.5561031	162.8731353	157.6811344	48.44024988	56.29884974	58.51603536	107.3789094	98.97257388	145.064895	167.7354538	140.734927	155.8374273	192.3861774	63.4903425	68.08570449	68.46605385	72.33911057	75.47130543	91.19695998	97.83863642	123.524594	68.27686395	100.4740563	67.57321651	101.6408572	89.52562643	103.2797088	121.096294	93.27171337	79.20887314	49.91299666	44.77179236	59.44733051	51.24265282	156.821895	256.8535377	188.960651	121.3536427	221.5203371	202.1348487	133.036575	123.4365187	131.6246796	88.48871945	72.09150896	75.96705857	73.60161463	93.72875989	61.2089221
PI4P 38:4	69.5952937	80.41161127	80.37617216	33.95386527	37.85637711	44.23622132	377.0941654	611.7595587	654.8098046	152.9484941	212.4056351	217.2947663	601.8266172	548.3980703	779.1237945	876.9401713	702.5843077	754.1733064	988.6346995	321.2453687	340.4676403	379.47654	379.68544	354.6450518	453.8122554	373.2236096	511.7332431	392.5030656	506.2978388	335.4312697	503.0296441	339.8497081	454.7211138	529.709789	404.8580939	347.6022885	182.3528085	236.0352156	256.775491	241.1979751	824.8891647	1469.181971	952.5264425	499.7414639	826.9130498	784.7136143	711.4255346	571.8531659	662.1635742	436.5117825	344.1748075	386.0129323	452.5548967	518.5421739	343.1230922
PI4P 38:5	24.670327	29.86686887	36.34494317	10.03477407	12.5351696	13.74227336	104.0764125	160.5252091	194.6151431	46.72030713	63.23969404	68.87851903	196.6810193	174.5334473	247.8254201	267.8915462	213.0826531	243.6367811	294.4412416	97.43950853	96.1329814	114.5818154	114.593867	86.56652826	122.2658582	141.227449	190.0780992	156.3789111	197.9964938	115.7252203	154.5856123	136.3744737	138.5690469	135.9738387	151.2887611	113.899651	67.02495426	71.63486777	99.16184498	75.21263793	254.993001	426.4427325	311.673517	183.1535467	226.042142	213.8555957	206.9655451	134.1022664	250.9161906	97.49112072	109.8359714	112.764302	130.850956	131.0577284	102.5543719
PI4P 38:6	1.670506845	1.587190103	1.558569373	2.957301309	2.989244894	5.630694303	5.365638783	6.138561356	5.855217207	13.57280388	19.69831699	18.26445868	11.76496004	8.736426693	15.27725255	11.05074261	8.999414675	12.03461595	13.96969958	49.6584537	58.92055282	59.89769223	52.73124809	41.22567695	62.36106948	5.872598806	10.27492711	6.529864137	11.25899754	8.854125147	7.31530928	3.812806614	7.360888779	5.148899092	5.509217171	4.573348791	12.68820363	18.01750821	14.77294607	25.33872573	9.883077742	21.13848888	13.00069874	18.48533476	23.04677383	24.57898275	8.442249678	9.573158879	11.66122364	44.69796909	59.91052986	76.54901011	46.99230426	64.4182055	36.5193617
PI4P 40:3	0.542163797	0.434501757	0.449862375	1.31748542	2.135029566	1.909536134	1.75061103	2.40146543	2.944278504	5.93368818	5.811222818	5.985949914	0.461553244	0.326927539	0.65082335	0.85569759	0.902630864	0.779873902	1.051081017	3.937884353	4.781438038	4.758140846	4.669189072	6.151105504	6.825616314	2.301146484	2.58322619	1.530450798	3.073016032	2.096517048	3.091167547	1.395035247	1.87368078	1.876992557	2.001167733	1.287621664	5.429395736	6.054652668	3.903897662	5.137884592	1.668968772	2.76991463	2.050077181	1.96084175	4.784454446	4.242526103	1.3689013	1.39175	1.131674548	5.966707818	6.223071636	6.034389803	5.439834385	4.238382015	3.934438887
PI4P 40:4	2.185608456	2.321237304	2.627953947	6.425680507	8.712147253	6.544953474	6.390331903	8.283158618	9.597894021	22.2993371	25.03726386	24.55838773	4.404931371	3.428929985	6.269330718	6.898791039	4.52598234	5.597041392	6.17761198	18.65864157	24.88610716	24.02042256	23.44026396	25.40261004	31.51829658	5.809881731	8.137001449	6.285383179	9.704636995	6.997938445	9.088304939	5.333873083	3.899503429	7.946223484	5.981881939	5.424123623	16.55564513	19.44434758	16.52578901	22.94853688	7.451288892	10.61990589	6.225747154	7.521618972	14.08975173	10.67548688	5.878746335	4.56415958	6.00072449	30.8419476	29.2094907	23.36759256	23.13240417	25.88105699	13.68961085
PI4P 40:5	2.619066878	2.790376532	2.790804641	9.239709475	7.873755926	8.17831947	8.640578511	10.81946784	10.543066	24.74898161	28.58265271	28.23976153	7.974337411	6.615990822	9.902119837	10.8992872	8.164653964	9.455603868	10.94893749	28.83525286	36.6219776	33.88050922	36.63437616	34.15520954	44.2487115	8.529522149	13.1617307	9.330282385	14.89139295	11.62121829	16.59698241	7.335886283	9.803996804	9.906830149	10.22205127	8.2924502	22.62693986	28.19786063	31.51561828	26.28186293	12.52476205	17.71884119	12.87197895	13.75017512	23.31615795	22.17334748	9.9362433	7.823716122	11.70375181	44.2094667	36.73766724	34.32171114	36.86634749	40.42255939	28.81821991
PI4P 40:6	1.908511981	2.084889722	2.026993118	8.036712737	7.918491182	11.32964969	6.50059912	7.843237385	6.97097404	23.8158012	34.70635529	32.80940276	9.17742242	7.468476931	12.43314913	11.03561254	8.372510266	9.609893286	12.41771702	44.59575558	66.58681502	58.63471358	55.62604629	54.21666128	70.73302001	6.54538197	12.43701013	7.867841745	11.42800152	10.380896	14.92747958	4.664603837	8.307829874	7.196579759	5.638944731	7.604885233	31.26583062	31.33188609	24.8277936	29.48240071	12.82461271	23.28526771	14.09481695	16.74303884	21.55554029	27.76856306	10.96781033	9.982879674	6.596118841	51.25785839	53.11569798	58.95616744	68.91549224	58.94617963	73.92154438
PI4P 40:7	0.912477687	1.06858759	0.996308137	4.244478621	4.014559419	4.750092575	1.815755025	2.143493484	2.223783298	10.62063835	13.6526158	14.06290541	4.235076815	3.53812114	5.374153395	4.16222987	3.24691745	3.983952654	5.451814756	23.730793	32.4881295	32.13018379	27.67514282	22.90248523	34.72521456	1.889494412	3.098180413	2.154988519	4.76543617	4.351153704	3.85102283	1.13196318	2.172914166	2.760995504	2.486444902	1.867190315	12.82193259	10.51927906	12.51878294	14.87569104	2.766722037	6.118003028	4.051669908	8.632560273	11.56908601	11.66694678	4.563794805	3.092416472	4.110347427	26.97580008	20.68694852	23.18853055	32.39978468	23.06377635	30.48675158
PI4,5P2 32:1	0.880606254	0.876357806	0.699560956	3.786085655	3.834091119	4.865032076	2.959591348	2.49342229	2.330107223	17.18083453	19.13708289	20.98529285	4.680633753	4.282684288	7.09590913	6.756390541	4.722798585	5.784207542	5.499348919	62.84306478	50.93775923	78.27819371	81.56052154	47.02610132	71.98518497	2.089608646	4.243753059	1.679102005	5.578270188	4.636799109	4.249959598	2.287013097	3.157323504	2.217049244	2.173384562	1.611809419	13.02333477	12.64081343	12.49669793	15.48672746	3.982054714	5.762782451	3.973327177	16.25769451	14.50271531	12.91996899	2.746266596	2.763452751	2.507078728	26.00529152	33.76001219	34.59671773	28.44136703	35.44300352	32.2194098
PI4,5P2 34:0	0.501635795	0.624677227	0.75283226	3.289151727	2.957817353	2.914386119	2.568924129	1.933667696	1.949422742	8.282211163	10.34254621	10.67722421	1.667390432	1.888019185	2.266595259	2.48635462	1.837969442	3.387351992	2.091317134	14.59856191	11.90967773	13.97935693	18.65477462	13.30818432	16.81149097	2.317471927	3.682500616	1.880498579	3.993061213	3.631321866	3.715995737	2.347640838	2.623637507	1.812529693	2.503761175	1.990054416	7.925620539	9.043685548	7.219080327	9.546585951	2.333428462	2.597555205	2.072505927	5.696061789	7.640682541	6.566094957	1.730416758	1.701680168	1.960753169	16.08516954	20.28410464	17.29603538	17.29769873	11.99490294	16.03155418
PI4,5P2 34:1	7.111649218	5.947632518	5.234767181	30.73444264	26.35922514	31.70304093	16.97226869	13.6249384	12.62023241	65.93594356	85.6469875	81.72406842	14.54640787	11.97707253	20.29323758	21.8763119	17.83609165	19.5379084	16.58842645	135.5523355	100.6382528	146.6639186	165.4638263	125.5495235	171.5736509	15.41889083	25.38069028	13.07620721	32.29796923	25.3429404	27.9955666	14.2879375	19.40552205	13.37987456	14.47663984	10.58989808	61.11021344	69.64707359	58.86327036	68.57931609	16.49391394	21.20332694	16.98672783	42.93030396	55.86877111	51.07416208	12.08939708	11.79088974	10.857063	103.9464382	119.3920022	94.58914565	97.76585148	104.8918333	108.949206
PI4,5P2 34:2	4.644510534	4.02585809	3.213213315	20.56432878	16.80486342	22.87718376	10.20004608	8.957521537	7.612767312	51.38314917	58.97163843	59.86678737	13.48052327	12.43289648	17.03786128	18.37434835	12.45275391	16.78376535	13.32723677	107.2874878	96.92379265	148.2019287	138.1199253	94.46185117	143.958501	8.729072284	14.24524458	7.177261442	21.32208064	14.91860222	16.47372916	8.166219816	10.72561367	8.271744736	8.178835661	6.307289431	39.47220929	44.34457869	36.58664083	47.14460277	11.09456095	13.89817753	10.51205545	41.70196908	35.67192117	30.74227532	7.840075927	8.08416581	6.816208709	59.70755152	59.32857457	66.98612305	57.37867905	66.95035226	54.81207201
PI4,5P2 36:1	3.697415692	3.373545753	3.374393919	16.1136816	16.76855039	16.2288961	9.23341448	6.697497585	5.800884421	23.85760699	28.05325119	25.40531338	3.779301627	3.937959228	4.843693962	6.043926507	5.061901877	6.642249691	5.449161956	25.82527061	21.87970136	31.74808395	32.61579819	33.44657821	31.59993026	8.658698037	12.59659367	7.946532573	12.6541479	8.944844671	12.68009342	8.831440856	10.48253253	6.994712282	8.130991487	7.485547845	23.99823025	29.31959203	20.52739084	24.02335208	4.904688605	6.050668449	5.592228033	10.48338324	15.2675945	15.09659384	4.961648051	4.135699015	4.599011763	29.33068081	26.19534877	27.11509039	25.33632014	24.76367059	28.18754319
PI4,5P2 36:2	10.06874523	9.452393486	8.628770168	47.59947399	37.75317807	39.76542858	26.95476174	23.47748808	19.74959269	73.59490944	92.47459703	83.38543539	17.65084616	17.98028459	23.63246446	27.39153043	23.43426316	28.31938194	24.45182536	92.57869804	74.73685226	111.7488517	110.4956298	98.90214467	121.6235235	25.2243692	36.8408882	24.17611088	42.9470639	33.42847293	41.00782028	22.11228422	26.85636852	21.47530317	22.8468522	21.99014939	63.69804391	87.15013965	56.87695536	59.89168754	18.70644995	22.69000669	20.44537339	40.47135951	49.08752574	43.70424637	17.51472962	14.80319544	13.24144998	70.00131274	62.31121329	71.1683992	53.19549087	67.06090436	67.36947153
PI4,5P2 36:3	8.338074816	7.639870547	6.56881521	16.67751988	12.85561964	15.02403408	31.10103232	29.67908179	26.97343235	35.92678409	39.77024272	41.99311554	52.20689158	51.9575106	75.4307337	77.86998982	75.29221551	77.59650488	73.7959858	74.27332347	64.63495235	86.53753169	102.3354007	68.14389901	95.14231724	30.22920436	48.57392541	23.74533225	34.82220425	24.59581054	32.70305846	27.98980683	31.81904742	27.15268217	29.9151994	23.01932204	28.87457585	28.90223129	30.49954647	33.29930761	45.71086634	66.73183953	48.05122682	58.88318944	67.07343263	61.29681674	42.9860163	45.22013593	39.25107355	49.58192795	59.54470781	60.20154173	52.4623548	59.38858856	56.19747111
PI4,5P2 36:4	17.02136849	13.802861	14.07210653	14.6558023	14.93272497	16.74202734	59.741801	60.32137735	53.21916015	46.57974002	46.35748309	55.15638226	193.802816	161.9408419	262.5248707	239.0355495	222.5576006	197.6781292	228.6652828	171.4451967	148.9906873	196.8350401	241.382025	140.2119299	200.6572062	57.83243636	94.14732987	52.2789876	59.48375827	47.15142126	57.74966425	55.28913107	76.11990143	69.36779843	69.60068278	59.00910508	40.87524991	43.69245253	57.70992617	53.12592716	147.6099453	191.7665001	136.5917339	155.4412293	151.1673015	152.0882606	140.0909567	136.9639232	162.6115904	108.4114272	143.7502184	169.2660098	166.6590794	167.7296474	153.2286926
PI4,5P2 38:2	4.024682007	3.87345252	3.80768749	3.474788901	3.990640627	3.335328094	20.77335513	17.83842588	18.12516981	8.244671348	7.763843384	9.088954047	19.26438961	22.49743188	23.65368508	27.16502578	31.52610882	34.32318634	30.76860917	13.22626476	11.28240515	14.5479952	15.40010941	16.77817482	17.9306745	18.50975973	29.50551765	13.00967981	15.91775591	10.20674148	17.65826794	18.50830187	19.58032197	17.27142316	18.30921	12.38133179	12.79261254	12.22345269	10.80299062	11.20959024	17.60464527	21.27588906	18.56708342	16.47652009	25.33480616	23.83727307	27.44026574	21.12406035	20.04737007	15.98802334	16.03708646	16.26673075	14.9020688	14.54865647	16.4738162
PI4,5P2 38:3	35.15840347	34.04042334	31.43950222	17.88436524	21.20724001	16.98339829	173.3381281	161.7896709	160.6063094	50.67200166	49.13016946	57.2399751	209.8540824	242.5230725	321.3351153	329.3508927	373.3766105	379.637889	386.1524035	107.7299161	109.1921965	132.316829	146.7606494	134.0712467	153.5262648	150.0171016	247.3165991	112.6539745	135.0613136	89.23105509	142.7382827	164.486428	153.987802	152.7653222	147.0830637	111.1650745	74.70411807	80.70713322	74.8605815	74.56064048	234.9126628	277.0016806	218.6465822	171.1252459	255.7640333	237.2912202	253.7944579	241.5058622	216.5496646	116.1083957	124.2766134	126.9088464	137.4630135	139.9589596	129.2151082
PI4,5P2 38:4	174.3736218	161.3921364	139.001558	89.55526821	89.05837901	68.01479742	635.0111726	606.1098301	624.3133261	204.0411272	192.319443	233.9598557	982.7435169	921.3401729	1441.337818	1268.860235	1451.242855	1445.792318	1529.590049	424.1282527	480.8919323	583.5643213	623.2534292	477.5326679	580.5359442	531.2962401	879.8414892	511.911822	520.0340497	400.835276	529.6589185	636.7596849	665.6053096	607.2830865	638.8456303	514.586328	276.2431077	345.8877138	308.4127808	265.1393633	1114.54429	1290.762019	1005.100202	742.5284332	1008.541942	995.9759191	1158.763222	1177.429954	1224.088198	526.8313369	612.5216041	589.2362402	629.2032524	581.5040503	494.4809089
PI4,5P2 38:5	42.81090661	38.85078858	33.1653231	18.22634347	16.996919	16.78960407	134.9687362	129.7964932	114.0808322	44.4454539	42.88217919	49.12506107	244.1337888	235.0875028	346.4669872	335.8397513	315.2475083	323.7838282	305.0978855	106.4048296	97.60986038	141.7337772	150.6932287	101.4323929	128.3485155	127.166797	200.0212967	123.7052197	126.9704048	95.22368059	123.8841471	122.7543333	131.4823127	151.6570495	128.0209375	119.052192	57.02580632	68.31673623	68.55990488	52.53760017	247.7020136	306.7184007	238.6286676	217.6017982	204.6491722	182.9300145	238.3383082	259.5180757	207.7271756	92.84935264	102.1013429	104.9983663	96.21332804	114.5319765	97.42197504
PI4,5P2 38:6	1.795585307	1.715722716	1.312359211	4.976714249	5.052541224	4.831653637	6.298015029	6.059201201	5.948800287	19.73514478	21.55275273	24.54250401	15.0268352	12.67828826	19.80591228	19.09895935	13.2622644	14.6267619	16.2564255	69.58072561	67.82290882	84.98134285	93.32001754	56.76047856	77.96927902	5.273563486	9.956855865	4.734194002	9.393753346	7.613033899	7.926380079	5.377343756	6.961952951	6.624448434	5.325812014	4.099858361	13.33200371	14.7641362	11.68508535	23.96451938	15.88473328	22.92625545	13.89100356	24.80531297	21.16017848	19.61428076	10.14057821	11.17705247	9.941376099	42.0717791	70.20007657	68.91461705	77.19491582	62.81570369	57.08554746
PI4,5P2 40:3	1.765436635	1.541224526	1.225678214	4.47912839	4.349305771	4.296452781	4.350700806	3.129070065	3.686540677	8.611717945	8.371679905	9.164387284	2.32759514	2.126563931	2.791796457	3.674940637	3.220578203	3.557931155	3.283293193	8.145058765	9.557860985	11.56193493	12.4092719	12.57764818	13.67587499	3.204214092	6.115060726	2.74936837	4.345423017	3.113150491	5.152033763	3.60903466	4.195198012	4.002375879	3.585794945	3.104350231	9.69968745	11.15135729	7.710319521	9.805449827	3.005860875	3.296176562	2.97973469	4.01597264	6.06471622	5.902054328	2.806747182	2.903284409	2.478527816	11.5454527	11.16760455	11.9264575	12.43959412	9.5826561	10.88959214
PI4,5P2 40:4	5.991406579	4.646127105	4.914597139	17.46399239	19.95979328	17.18914897	12.29574887	10.33259856	11.34484717	28.80442667	31.95784315	33.89989691	9.613051067	7.545869192	12.55145599	12.89246276	11.20956982	11.97728594	13.38647167	28.6086887	39.49960392	49.9919862	49.47418502	44.40540683	51.34077571	10.36420724	18.3155515	9.752558954	15.37118994	11.69074689	16.59396544	10.98035743	15.33869273	14.65186941	11.96607976	12.61698347	31.95814734	35.63217323	27.46667841	33.37775121	12.63504711	14.85161006	10.47183431	14.11629737	19.90154751	20.36164531	11.95499578	10.53714488	10.50467788	43.47292628	47.93835278	47.35266	56.96898536	46.49821361	41.79287279
PI4,5P2 40:5	5.830247849	5.345924398	4.921987678	16.36060023	15.31414628	14.86860016	13.77418121	11.3994655	11.51253511	29.69947709	31.73998348	32.7692328	14.69206757	11.48195491	18.0879075	19.35494197	17.22636842	17.97444362	18.58815197	40.49297225	48.94709749	58.10553508	59.70160071	51.93828836	63.43571837	11.61574924	19.42729453	10.15584693	17.68487411	13.62225089	17.52987494	12.41689806	15.32776774	15.241269	13.48450276	12.47511154	29.37031687	35.42349286	27.78705179	37.10382214	18.44770438	21.35520115	15.91230202	21.05161257	25.81845951	26.26557017	14.7147025	14.74157471	16.13255133	51.50617007	49.08385896	61.17740617	59.25464488	55.82881092	51.97022771
PI4,5P2 40:6	3.089804003	3.617776194	3.76806083	14.28338707	12.97531815	14.15644375	10.71869249	9.172068012	9.665489053	37.45461238	42.39018614	45.97255256	15.38300521	12.2605964	22.4680599	19.47177379	14.63182289	17.31257726	19.97140626	82.50611714	82.12465118	93.68987261	103.7809778	82.62855887	97.77296917	8.762979875	17.12985031	7.807787213	16.04838965	11.6905418	16.21195848	10.42797135	13.30664369	10.71750117	10.34693218	7.365317395	31.24103769	35.31915268	29.11126179	43.00670294	19.75278182	24.66099641	16.33108601	24.1310936	29.77138086	27.26069585	15.7473525	15.10418901	14.54810432	73.90584287	85.15649683	97.84202711	112.7520153	95.42857343	76.14366582
PI4,5P2 40:7	0.831138413	0.942274761	0.745638554	4.435245327	3.580545473	4.26626273	2.723798677	2.705182685	2.564720463	14.86998474	16.17234238	17.09413942	5.050252639	4.918627084	8.152429723	7.112952495	4.508512085	5.776930658	5.999824003	33.02243033	32.19213032	38.1261292	45.30848449	28.99774568	38.7214678	2.38347444	4.098491625	2.062981578	5.922668225	4.445207366	4.637857531	2.351009046	3.031686063	2.61451615	2.221228737	1.973462614	10.14866045	12.36952895	10.01700791	13.94923292	6.348384289	7.916864968	5.385349858	15.02481024	11.87437071	10.47857813	4.130600003	4.164139352	3.703902091	22.99749559	33.95453211	28.34653837	27.64354248	30.33549647	26.23377523
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name
PI4P 32:1
PI4P 34:0
PI4P 34:1
PI4P 34:2
PI4P 36:1
PI4P 36:2
PI4P 36:3
PI4P 36:4
PI4P 38:2
PI4P 38:3
PI4P 38:4
PI4P 38:5
PI4P 38:6
PI4P 40:3
PI4P 40:4
PI4P 40:5
PI4P 40:6
PI4P 40:7
PI4,5P2 32:1
PI4,5P2 34:0
PI4,5P2 34:1
PI4,5P2 34:2
PI4,5P2 36:1
PI4,5P2 36:2
PI4,5P2 36:3
PI4,5P2 36:4
PI4,5P2 38:2
PI4,5P2 38:3
PI4,5P2 38:4
PI4,5P2 38:5
PI4,5P2 38:6
PI4,5P2 40:3
PI4,5P2 40:4
PI4,5P2 40:5
PI4,5P2 40:6
PI4,5P2 40:7
METABOLITES_END
#END