#METABOLOMICS WORKBENCH shancock_20250218_223155 DATATRACK_ID:5646 STUDY_ID:ST003757 ANALYSIS_ID:AN006169 PROJECT_ID:PR002341 VERSION 1 CREATED_ON February 20, 2025, 2:01 pm #PROJECT PR:PROJECT_TITLE Untargeted lipidomics of gemcitabine-resistant PDAC cells PR:PROJECT_SUMMARY Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive disease with few PR:PROJECT_SUMMARY treatment options and poor survivability. In this work we sought to characterise PR:PROJECT_SUMMARY metabolic adaptations to gemcitabine (GEMC)-based chemotherapy exposure to PR:PROJECT_SUMMARY discover new therapeutic targets for improving treatment efficacy. We show that PR:PROJECT_SUMMARY GEMC resistance (GEMR) upregulates de novo lipogenesis in Panc1 and MiaPaCa2 PR:PROJECT_SUMMARY cells. PR:INSTITUTE Victor Chang Cardiac Research Institute PR:LAST_NAME Hancock PR:FIRST_NAME Sarah PR:ADDRESS Lowy Packer Building, 405 Liverpool Street, Darlinghurst, NSW, 2010, Australia PR:EMAIL s.hancock@victorchang.edu.au PR:PHONE +61414537526 #STUDY ST:STUDY_TITLE Untargeted lipidomics of gemcitabine-resistant PDAC cells ST:STUDY_SUMMARY In this work we developed gemcitabine (GEMC) resistant (GEMR) PDAC cell lines ST:STUDY_SUMMARY from Panc1 and MiaPaCa2 cells. This dataset contains results and raw data for ST:STUDY_SUMMARY untargeted lipidomics of Panc1 cells, including control (CON), and GEMR cells ST:STUDY_SUMMARY alongside internal quality controls (iQC) and blank samples for background ST:STUDY_SUMMARY subtraction (as described under sample prep). Untargeted lipidomics enabled the ST:STUDY_SUMMARY quantification of more than 400 distinct molecular lipid species within Panc1 & ST:STUDY_SUMMARY MiaPaCa2 control (CON) & GEMR cells from 12 different classes of lipid, with 77 ST:STUDY_SUMMARY molecular species being differentially abundant between Panc1 control and GEMR ST:STUDY_SUMMARY cells following unpaired t test. Data were analysed using MS-DIAL 4.9. ST:INSTITUTE Victor Chang Cardiac Research Institute ST:LAST_NAME Hancock ST:FIRST_NAME Sarah ST:ADDRESS Level 7 Lowy Packer Building, 405 Liverpool Street, Darlinghurst, NSW 2010 ST:ADDRESS Australia ST:EMAIL s.hancock@victorchang.edu.au ST:PHONE +61414537526 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:CELL_STRAIN_DETAILS Panc1 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Blank_cells_neg Sample source:Blank | group:Blank RAW_FILE_NAME(Raw file name)=Blank_cells_neg.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - Blank_cells_neg_20210814030609 Sample source:Blank | group:Blank RAW_FILE_NAME(Raw file name)=Blank_cells_neg_20210814030609.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - Blank_cells_pos Sample source:Blank | group:Blank RAW_FILE_NAME(Raw file name)=Blank_cells_pos.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - Blank_cells_pos_20210813215440 Sample source:Blank | group:Blank RAW_FILE_NAME(Raw file name)=Blank_cells_pos_20210813215440.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - iQC_cells_neg Sample source:pool | group:iQC RAW_FILE_NAME(Raw file name)=iQC_cells_neg.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - iQC_cells_neg_20210814033722 Sample source:pool | group:iQC RAW_FILE_NAME(Raw file name)=iQC_cells_neg_20210814033722.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - iQC_cells_pos Sample source:pool | group:iQC RAW_FILE_NAME(Raw file name)=iQC_cells_pos.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - iQC_cells_pos_20210813222552 Sample source:pool | group:iQC RAW_FILE_NAME(Raw file name)=iQC_cells_pos_20210813222552.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - Panc1_CON_p1_neg Sample source:Panc1 cells | group:CON RAW_FILE_NAME(Raw file name)=Panc1_CON_p1_neg.mzML; protein amount=149.2651285 SUBJECT_SAMPLE_FACTORS - Panc1_CON_p1_pos Sample source:Panc1 cells | group:CON RAW_FILE_NAME(Raw file name)=Panc1_CON_p1_pos.mzML; protein amount=149.2651285 SUBJECT_SAMPLE_FACTORS - Panc1_CON_p2_neg Sample source:Panc1 cells | group:CON RAW_FILE_NAME(Raw file name)=Panc1_CON_p2_neg.mzML; protein amount=100.2281304 SUBJECT_SAMPLE_FACTORS - Panc1_CON_p2_pos Sample source:Panc1 cells | group:CON RAW_FILE_NAME(Raw file name)=Panc1_CON_p2_pos.mzML; protein amount=100.2281304 SUBJECT_SAMPLE_FACTORS - Panc1_CON_p3_neg Sample source:Panc1 cells | group:CON RAW_FILE_NAME(Raw file name)=Panc1_CON_p3_neg.mzML; protein amount=79.09305324 SUBJECT_SAMPLE_FACTORS - Panc1_CON_p3_pos Sample source:Panc1 cells | group:CON RAW_FILE_NAME(Raw file name)=Panc1_CON_p3_pos.mzML; protein amount=79.09305324 SUBJECT_SAMPLE_FACTORS - Panc1_GEMR_p1_neg Sample source:Panc1 cells | group:GEMR RAW_FILE_NAME(Raw file name)=Panc1_GEMR_p1_neg.mzML; protein amount=184.7074903 SUBJECT_SAMPLE_FACTORS - Panc1_GEMR_p1_pos Sample source:Panc1 cells | group:GEMR RAW_FILE_NAME(Raw file name)=Panc1_GEMR_p1_pos.mzML; protein amount=184.7074903 SUBJECT_SAMPLE_FACTORS - Panc1_GEMR_p2_neg Sample source:Panc1 cells | group:GEMR RAW_FILE_NAME(Raw file name)=Panc1_GEMR_p2_neg.mzML; protein amount=167.9988515 SUBJECT_SAMPLE_FACTORS - Panc1_GEMR_p2_pos Sample source:Panc1 cells | group:GEMR RAW_FILE_NAME(Raw file name)=Panc1_GEMR_p2_pos.mzML; protein amount=167.9988515 SUBJECT_SAMPLE_FACTORS - Panc1_GEMR_p3_neg Sample source:Panc1 cells | group:GEMR RAW_FILE_NAME(Raw file name)=Panc1_GEMR_p3_neg.mzML; protein amount=129.6441352 SUBJECT_SAMPLE_FACTORS - Panc1_GEMR_p3_pos Sample source:Panc1 cells | group:GEMR RAW_FILE_NAME(Raw file name)=Panc1_GEMR_p3_pos.mzML; protein amount=129.6441352 #COLLECTION CO:COLLECTION_SUMMARY Samples were obtained from established immortalised adherent human pancreatic CO:COLLECTION_SUMMARY ductal adenocarcinoma cells (Panc1). Cells were cultured in high glucose DMEM CO:COLLECTION_SUMMARY containing 4.5 g/L glucose and 4 mM glutamine and were supplemented with 10% CO:COLLECTION_SUMMARY FCS. Cells were cultured under 5% CO2 at 37ºC and routinely passaged every 2-3 CO:COLLECTION_SUMMARY days (when ~80-90% confluent). Cell lines were regularly screened for mycoplasma CO:COLLECTION_SUMMARY infection. CO:SAMPLE_TYPE Pancreas #TREATMENT TR:TREATMENT_SUMMARY Panc1 GEMR cells were produced by serial treatment with escalating doses of TR:TREATMENT_SUMMARY gemcitabine for approximately 12 weeks. The final concentration of GEMC for TR:TREATMENT_SUMMARY Panc1 cells was 150 nM, resulting in a half-maximal inhibitory concentration of TR:TREATMENT_SUMMARY 720.5 nM (approximately 2.5 times increase over control (CON) cells, 273.3 nM) #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Lipids were extracted from cells grown to 80-90% confluency in 6 well plates SP:SAMPLEPREP_SUMMARY using a modified methyl tert-butyl ether (MTBE) method. Adherent cells were SP:SAMPLEPREP_SUMMARY washed with ice-cold PBS and scraped into methanol containing 0.01% butylated SP:SAMPLEPREP_SUMMARY hydroxytoluene and 100 pmol each of internal standards (phosphatidylcholine SP:SAMPLEPREP_SUMMARY 19:0/19:0, phosphatidylethanolamine 17:0/17:0, phosphatidylserine 17:0/17:0, SP:SAMPLEPREP_SUMMARY lysophosphatidylcholine 17:0, lysophosphatidylethanolamine 14:0, SP:SAMPLEPREP_SUMMARY phosphatidylglycerol 17:0/17:0, phosphatidic acid 17:0/17:0, ceramide SP:SAMPLEPREP_SUMMARY d18:1/17:0, dihydrosphingomyelin 12:0, cholesteryl ester 22:1, diacylglycerol SP:SAMPLEPREP_SUMMARY 17:0/17:0, D5-triacylglycerol 16:0/16:0/16:0 and cardiolipin SP:SAMPLEPREP_SUMMARY 14:0/14:0/14:0/14:0). An empty well was also scraped for use in background SP:SAMPLEPREP_SUMMARY subtraction. To this, MTBE was added at a ratio of 3:1 v/v, and extracts were SP:SAMPLEPREP_SUMMARY rotated overnight at 4ºC. Following overnight rotation, 1 part of ice-cold 150 SP:SAMPLEPREP_SUMMARY mM ammonium acetate was added, samples were vortexed vigorously and centrifuged SP:SAMPLEPREP_SUMMARY at 2000 x g for 5 min. The upper organic phase containing lipids was transferred SP:SAMPLEPREP_SUMMARY into a 1.5 mL autosampler vial and dried under N2 at 37ºC Dried lipids were SP:SAMPLEPREP_SUMMARY reconstituted in chloroform:methanol:water (60:30:4.5 v/v/v), transferred to a SP:SAMPLEPREP_SUMMARY sleeved vial, and stored at -20ºC until analysis. The aqueous phase containing SP:SAMPLEPREP_SUMMARY the protein pellet was dried under N2 and digested with 1M NaOH overnight at SP:SAMPLEPREP_SUMMARY 4ºC. Digested protein was diluted 1:2 v/v with water and protein concentration SP:SAMPLEPREP_SUMMARY was determined using a Pierce BCA assay kit (ThermoFisher Scientific) #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) CH:SOLVENT_A Acetonitrile:water (3:2 v/v), 10 mM Ammonium Formate, 0.1% Formic acid CH:SOLVENT_B Acetonitrile:isopropanol (1:9 v/v), 10 mM Ammonium Formate, 0.1% Formic acid CH:FLOW_GRADIENT As described by Hu et al. 2008 J Proteome Research CH:FLOW_RATE 0.26 ml/min CH:COLUMN_TEMPERATURE 60 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF hybrid Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Source conditions were as follows: a spray voltage 4.0 kV in positive ion mode MS:MS_COMMENTS and 3.5 kV in negative ion mode, capillary temperature 290°C, S lens RF 50, and MS:MS_COMMENTS auxiliary gas heater temperature of 250°C. Nitrogen was used as both source and MS:MS_COMMENTS collision gas, with the sheath gas flow rate set at 20 and the auxiliary gas MS:MS_COMMENTS flow rate at 5 (arbitrary units). Data were acquired in full scan/data-dependent MS:MS_COMMENTS MS2 mode (full-scan resolution 70,000 FWHM, max ion injection time 50 ms, scan MS:MS_COMMENTS range m/z 200–1,500), with the 10 most abundant ions being subjected to MS:MS_COMMENTS collision-induced dissociation using an isolation window of 1.5 Da and a MS:MS_COMMENTS normalized stepped collision energy of 15/27 eV. Product ions were detected at a MS:MS_COMMENTS resolution of 17,500. An exclusion list for background ions was developed using MS:MS_COMMENTS extraction blanks, and mass calibration was performed in both positive and MS:MS_COMMENTS negative ionization modes before analysis to ensure mass accuracy of 5 ppm in MS:MS_COMMENTS full-scan mode. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg protein MS_METABOLITE_DATA_START Samples Panc1_CON_p1_neg Panc1_CON_p2_neg Panc1_CON_p3_neg Panc1_GEMR_p1_neg Panc1_GEMR_p2_neg Panc1_GEMR_p3_neg Factors Sample source:Panc1 cells | group:CON Sample source:Panc1 cells | group:CON Sample source:Panc1 cells | group:CON Sample source:Panc1 cells | group:GEMR Sample source:Panc1 cells | group:GEMR Sample source:Panc1 cells | group:GEMR CL 16:0_18:1_18:1_18:1 0.046299888 0.060642026619088615 0.0883901 0.042934139984523646 0.034097760004876874 0.041595911461150534 CL 16:1_16:1_16:1_18:1 0.017799202711822427 0.018726381276362274 0.022957167072038306 0.12613146067484174 0.088290524 0.080134336 CL 16:1_18:1_16:1_18:1 0.051149179 0.063528391 0.075549561 0.17620738141581127 0.13978199412378778 0.14492574254622492 CL 18:1_18:1_18:1_18:1 0.1318863527650121 0.1588360871203502 0.2319986636237598 0.058186688 0.056360896226513864 0.088919528 PC 14:0_14:0 0.060606894 0.057103949 0.099689509 0.1531103886895184 0.15296765520789185 0.089633567 PC 14:0_16:0 0.4398476148937144 0.4804030260934881 0.6993402526518969 0.839537178 0.6848980409803017 0.6083834084706281 PC 14:0_16:1 0.092109915 0.091749474 0.15982798165438622 0.37289235556409356 0.32922311368474144 0.15865965750027627 PC 14:0_20:4 0.006678793 0.007682983 0.014371940210625815 0.015662937 0.012189175196620848 0.007264644 PC 16:0_16:0 0.6967508933816068 0.8008663591758123 1.216787949916214 1.291630815715034 0.9677322294159371 0.883668973 PC 16:0_16:1 1.1003912052207843 1.1917721826081509 1.7430210044236918 2.4303729806855947 1.7513907794081358 1.5638586005918063 PC 16:0_17:1 0.094208212 0.11837004527612105 0.1374983452071948 0.095098817 0.067320749 0.083377863 PC 16:0_18:0 0.14065010080953408 0.14390458031029515 0.2280976402618228 0.091379728 0.083622269 0.1143682049857736 PC 16:0_18:1 3.456733621547543 3.4214325212208045 5.204970513085755 3.810401712650357 3.052602720183229 3.9128673053036214 PC 16:0_19:1 0.097215539 0.10839752059254726 0.17320000993918783 0.081635566 0.064485997 0.082247692 PC 16:0_20:4 0.11155784448421945 0.13626066975551043 0.22665255273277798 0.098868681 0.080642692 0.080288648 PC 16:0_22:6 0.060840626 0.079210124 0.12483859957708132 0.035088994682741945 0.033465670170601515 0.043272414 PC 16:1_16:1 0.072760925 0.07981419 0.16894376690863086 0.30923679016225003 0.3556530341197348 0.19542972132933695 PC 16:1_18:1 0.8437029497330439 0.9134353123612106 1.388403356 2.165473934779865 1.5529397478468256 1.3517511295602789 PC 16:1_18:2 0.041463445 0.040199651822382214 0.079589713 0.1359729758618784 0.14048596459262716 0.084348541 PC 16:1_20:4 0.008247041 0.010169955200033888 0.016673177123925718 0.014686955752213298 0.013151579830629792 0.008093617 PC 16:1_22:6 0.008157007 0.009895879 0.017815905451663602 0.009633175 0.010018306543327934 0.006217005 PC 17:0_18:1 0.027031573 0.035460005737537145 0.061862452 0.016812296096307133 0.026535286 0.030473727409370908 PC 17:0_19:0 0.004574204 0.007704272 0.005754765 0.002762366 0.002903585 0.004273912 PC 17:1_18:1 0 0 0.020562859 0 0 0.011563014183447141 PC 18:0_18:1 0.7204545339224534 0.7313520913308517 1.0868013368926515 0.47273925693017377 0.4028179706646606 0.5843644135869693 PC 18:0_20:4 0.052686053631482564 0.073287397 0.12112432561556354 0.028164837 0.026967372454711543 0.034046630031943366 PC 18:0_22:5 0.036010421 0.046410579303686805 0.074273619 0.027490762733743474 0.024806015 0.024990366112063442 PC 18:0_22:6 0.039661131 0.050698152 0.082282832 0.014375013485458702 0.016369211488730898 0.021578749211164396 PC 18:1_16:2 0.005817181 0.006406166 0.009594098 0.013587757594538742 0.009575407 0.005827671 PC 18:1_18:1 2.0012533679739652 2.130248483568972 3.182465261 2.588892972016139 2.3444871269565613 2.940372265415712 PC 18:1_18:2 0.29800917380385167 0.31815574310865247 0.5038996532129076 0.6130542040122308 0.44075513003054984 0.4153789027588308 PC 18:1_19:1 0.063639655 0.071611988 0.1120042827328695 0.045406926 0.042404586 0.066764396 PC 18:1_20:1 0.27048205600234243 0.29850990450805315 0.414295317 0.25355380386704857 0.20989300168499345 0.30429447958316197 PC 18:1_20:2 0.15266107626820843 0.15287852501637444 0.232953798 0.255150555 0.19068375778459304 0.18590713482598306 PC 18:1_20:3 0.10653917361139767 0.11864138022748941 0.19648115031572683 0.1335500116794015 0.094759129 0.098461215 PC 18:1_20:4 0.16789993898347422 0.21714145948001237 0.339101161 0.15145540405272284 0.13268027061379867 0.12785527756811738 PC 18:1_22:2 0.036614631 0.037018605 0.051427218 0.038794784 0.027289857059594535 0.030398458422931474 PC 18:1_22:3 0.066557362 0.072465627 0.10272465762829659 0.092638055 0.071993164 0.065837403 PC 18:1_22:4 0.024433144 0.030874576599001387 0.052500501 0.020284215 0.025246347 0.020924627669025725 PC 18:1_22:5 0.042637696213240574 0.05567618 0.086621862 0.044406857439944124 0.039471575 0.040672287 PC 18:1_22:6 0.091345628 0.1210068817422878 0.18859990835828028 0.048272198 0.045543595979990574 0.051653889175881325 PC 18:1_24:1 0.012211937 0.011535981039744932 0.015084490375037986 0.007848136 0 0.007320644 PC 18:1_24:3 0.016276697421473474 0.015569697995082893 0.023297979 0.010566000558181096 0.007718061 0.008707365 PC 18:1_24:5 0.018602880711066676 0.020902995 0.032243110355375866 0.008166102 0.008171059 0.008076418 PC 18:1_24:6 0.021969057 0.027995183518601717 0.039831426349151225 0.009432655 0.009454919 0.013662346012645024 PC 18:2_18:2 0.017418009429420865 0.020331236635187742 0.03550803 0.031730515 0.020931852 0.017790188 PC 20:1_20:1 0.036117002 0.040299042 0.051079185 0.027844252 0.020018198 0.02985903 PC 20:1_22:6 0.009213631 0.009705032 0.013751836 0 0 0.004099977 PC 36:6 0.004388398 0.006167572 0.009466302 0.004132937 0.00497597 0.004546026 PC 38:3 0.021045353 0 0.038697118 0 0 0 PC 42:6 0.008979567 0.013144995134624115 0.016226227508851155 0.006592679 0.004790181 0.007818194 PC O-16:0_14:0 0.40573862584985526 0.40172950821225933 0.6350621376110658 0.43956684808021185 0.454671618 0.6266296114360201 PC O-16:0_16:0 0.271521785 0.2912799290796896 0.5004241218244379 0.3168384495600505 0.46135173341142527 0.4811421307972771 PC O-16:0_16:1 0.6983331229022212 0.7185361567248414 1.0524805263811332 0.8306561446308165 0.7877397522563699 1.035681147704086 PC O-16:0_20:0 0.013022261798972794 0.012742306557787795 0.00871995 0.010585043668083235 0.009967902 0.012634656 PC O-16:0_20:1 0.3846120028318584 0.34041211878800187 0.480069864 0.3161957862500205 0.32744265919656024 0.5030801159838425 PC O-16:0_20:3 0.035157723 0.040563544 0.043419948 0.040314657 0.031877941702075425 0.063894515 PC O-16:0_20:4 0.2506169769169586 0.2957854824660315 0.4148205596019316 0.1283876829208328 0.098067231 0.19715278929771868 PC O-16:0_20:5 0.026527506152028656 0.033239256 0.05116984 0.016323092752531587 0.016823204704315562 0.025498339 PC O-16:0_21:3 0.016116972 0.017626980584684933 0.024707581069875178 0.024377362 0.022305075 0.022689169 PC O-16:0_22:1 0.096136406 0.090395923 0.12058155683324422 0.09830323 0.096742328 0.12128436106722243 PC O-16:0_22:2 0.15933932892515054 0.16215582287710764 0.21248697233913716 0.1932281993390668 0.18975240494186765 0.2376242480974134 PC O-16:0_22:3 0.23910557616899622 0.22115164603306367 0.2975112492940522 0.3159183916898223 0.27590033750472087 0.305642048 PC O-16:0_22:4 0.1499727304314284 0.16676105290376256 0.25481257487810394 0.20765781654873108 0.20839780134904493 0.21992794072767355 PC O-16:0_24:3 0.072312078 0.075399141 0.10211143523235129 0.066464847 0.066051243 0.080221046 PC O-16:0_24:5 0.060704443 0.069083289 0.10502180586665703 0.037406701 0.039263897 0.049960418 PC O-16:0_24:6 0.064758613 0.074433958 0.1124564699067508 0.027621207925508682 0.035243196 0.054279606 PC O-16:1_14:0 0.06042058 0.069418945 0.088451527 0.084602018 0.089540357 0.11965140972962779 PC O-16:1_16:0 0.17646611408453972 0.18459105145179625 0.25529611982342165 0.26485820973024415 0.22809735595796246 0.28118707447064445 PC O-16:1_16:1 0.083853313 0.094323167 0.13080978831447007 0.16458352365899723 0.14532326714850227 0.1764304926324007 PC O-16:1_18:1 0.17042696265195528 0.17162271517993152 0.2692281388849845 0.28885445628572143 0.23847956065542825 0.35735817691126653 PC O-16:1_18:2 0.030652487 0.033754819312546265 0.046709957 0.083172299 0.070666212 0.072695879 PC O-16:1_20:1 0.068588063 0.063308998 0.08412122 0.078023919 0.068809819 0.11299002914917007 PC O-16:1_20:3 0 0.018529741392832925 0 0 0.015163064467459467 0.014822026 PC O-16:1_20:4 0.048452571 0.06534334 0.10065115878276695 0.038763855087048625 0.039923117 0.047762428143321205 PC O-16:1_22:1 0.013157051619166395 0.012840891300082884 0.017930422 0.017053208207300535 0.014393075580732113 0.019517257449045176 PC O-16:1_22:4 0.023525335 0.029963279 0.046040823 0.018402424 0.015153274146377613 0.017971026 PC O-16:2_18:0 0.2192171934671308 0.24296977479418622 0.3331842735902263 0.3660125795243284 0.3231914544577897 0.38438827870127834 PC O-18:0_16:0 0.18303409102609655 0.1772044705502091 0.25869089143416185 0.083390269 0.091354901 0.1477717131247352 PC O-18:0_24:5 0.012819751 0.014470419428974184 0.020204074577587827 0.007044005 0.007572744 0.009247931 PC O-18:1_16:0 1.7097325580495508 1.7416498406485479 2.595512658 2.0461245431928954 1.8444021333052836 2.544765977583617 PC O-18:1_16:1 0.068159055 0.072942186 0.10526079623818628 0.098914244 0.094545073 0.1390379743216987 PC O-18:1_18:1 0.3622474873129015 0.3631437430796613 0.5376582765520359 0.571463679 0.5143631300589254 0.6954832573546826 PC O-18:1_20:4 0.18642516080450217 0.22988231244856375 0.3441164442386434 0.19882943701770822 0.20270528991595838 0.24902304640784548 PC O-18:1_22:1 0.048298384 0.046863369 0.062316502841337267 0.053120343 0.052560685 0.063373064 PC O-18:1_22:3 0.12209444744926914 0.12581942225637455 0.18497722972625988 0.13880775812477256 0.13682260769643503 0.15033856044487023 PC O-18:1_22:4 0.047647361 0.05726064 0.078636944 0.051007244202061784 0.054309027 0.062449244 PC O-18:1_24:2 0.007264195 0.00740762 0.009548982 0.007225662 0.006101881 0.006855975 PC O-18:1_24:3 0.014755660488442872 0.015780834 0.022220731830605314 0.010715850929942232 0.011163774 0.013422244431613719 PC O-18:1_24:4 0.008615363 0.009279512 0.013163641025697925 0.004893477 0.005765461 0.006275938 PC O-18:1_24:5 0.015979336 0.018616096877327043 0.029950546 0.00677737 0.008611174 0.011902764073345175 PC O-18:1_24:6 0.018146451099408305 0.021968654 0.030313075566881214 0.007572454 0.010861803196481782 0.018903083829890997 PC O-18:2_16:1 0.003081253 0.003298037 0.006888803 0.007059158 0.010377396591763448 0.008819932 PC O-18:2_18:1 0.15947329268431215 0.1576623622613145 0.22712988018893152 0.24871324213612298 0.1890719843232047 0.2334071001641511 PC O-18:2_22:1 0.009885193 0.010583794785378459 0.004467564 0.012694886079389572 0.012426661 0.013910233 PC O-18:2_22:3 0.02957352 0.036586039 0.051889621 0.040590714175236686 0.039186811 0.038241390682425595 PC O-18:2_22:4 0.070654712 0.074967377 0.12022401741560576 0.04306874 0.05039251 0.056777577291411634 PC O-18:2_22:6 0.025118502316301822 0.030450333272854596 0.045656014 0.022482214 0.026925228984117505 0.034878388 PC O-19:1_16:0 0.042618086 0.040584955335464086 0.066799594 0 0.030862795228914153 0.043902359 PC O-19:1_18:1 0.018382096 0.017900023 0.026381628763745203 0.015628258029662286 0.016368673534471728 0.021315487 PC O-20:1_18:1 0.017945304093987743 0.017484279750283615 0.024346618 0.018904588103864865 0.026249752633853186 0.03283406 PC O-22:5_16:0 0.059916614 0.07403822 0.10962702800368447 0.059153373 0.058769954 0.062519487 PC O-24:1_16:0 0.032874183 0.03019082 0.039740051 0.026303605977456422 0.025873030632333864 0.028636823640448613 PC O-24:1_18:1 0.010314413141761382 0.009668798 0.013123013338351992 0.008932327 0.007879289 0.009155287 PE 14:0_16:0 0.005524023 0.006041889 0.008711653 0.022321874 0.017285316 0.015199555191177725 PE 14:0_16:1 0.002872768 0.002562769 0.003151439 0.014852143898814344 0.010251070228727881 0.005654713 PE 16:0_16:0 0.023993708579294556 0.026820996559297813 0.033980949 0.058441959 0.043431816443345664 0.039699243 PE 16:0_16:1 0.069093235 0.083845375 0.097465834 0.2389904190388947 0.16586811882563682 0.1468298160667831 PE 16:0_18:1 0.2565801761876512 0.3315844619191415 0.37922920807378807 0.6323031723314246 0.4802624230461178 0.5478720014047371 PE 16:0_20:3 0.006490918 0.007600641 0.009709504 0.013417559 0.009811805 0.006228041 PE 16:0_20:4 0.028788781614265787 0.041199802 0.0546691 0.049909222 0.033998446652239736 0.032177986735622936 PE 16:1_16:1 0.013687075635384518 0.014683527501632434 0.014529572260314667 0.060875499 0.038688414718546636 0.024306403205816566 PE 16:1_18:1 0.1790448789462118 0.21289818530613958 0.25974677472768026 0.6367090645508514 0.42716455759870986 0.39523834956925785 PE 16:1_18:2 0.004178893 0.005714569 0.005224106 0.020802928958961985 0.012622709941871637 0.008194113 PE 16:1_22:6 0.006597273 0.008270204 0.010721059676143039 0.006518517 0.005461616 0.002888351 PE 17:0_17:0 0.02993867 0.042883536217758214 0 0 0 0.015338774824725834 PE 17:0_19:0 0.002560563 0.003016497 9.150082517270637e-4 9.49776844439705e-4 0.001513831 0.002175811 PE 17:1_18:1 0.017798756434927628 0.022806380340569483 0.029959387663034506 0.025185324 0.018023716 0.021966584 PE 18:0_18:1 0.4867702205070294 0.5862676709660869 0.7708826533245208 0.5172686376830402 0.4422464963685764 0.6578529262299212 PE 18:0_20:1 0.021243282450583555 0.027210543 0.033212173 0.018471981 0.015352860547526603 0.025580008 PE 18:0_20:3 0.056225626 0.065847242 0.089035 0.073173307 0.047180212 0.055500456 PE 18:0_20:4 0.2794323521381311 0.3577562776534484 0.5201958372865573 0.2958910520278875 0.2571740580987628 0.3031305695071255 PE 18:0_22:3 0.017487255205896023 0.024054886949581574 0.028251867763103174 0.023956627733978934 0.018149730386742096 0.020512178 PE 18:0_22:5 0.051791338 0.06332071 0.084435075 0.055091059844478006 0.041865485450994146 0.049283029656046105 PE 18:0_22:6 0.023656601181835733 0.027758768787327994 0.046197089 0.020409279210156672 0.020180747163192986 0.021054621078806388 PE 18:0_24:5 0.008306173 0.010023588326721053 0.013336140994206006 0.005036599 0.004507761 0.005918582 PE 18:1_18:1 0.6143367191568635 0.719930442 0.9398381637530937 1.4337462829548486 0.9898771588027596 1.1969939218242984 PE 18:1_18:2 0.07162819 0.089384535 0.11552896700326454 0.21256484248084298 0.14593638222295788 0.12138551243094341 PE 18:1_19:1 0.024182268830549886 0.029199574 0.036748143 0.032551058 0.022459747552362585 0.029852398 PE 18:1_20:1 0.11465747888463867 0.13431192267132963 0.170894299 0.17875604222358013 0.13424845735692753 0.17101445523248854 PE 18:1_20:2 0.049198831654917376 0.063670023 0.098840032 0.14919334266502454 0.085845742 0.087400369 PE 18:1_20:3 0.066279237 0.093781669 0.12100986271721566 0.11203925372729837 0.077700013 0.079907428 PE 18:1_20:4 0.14157611694304265 0.18143166556482646 0.25408398153263617 0.20611840681089183 0.1678905908928485 0.16735285548344211 PE 18:1_22:1 0.033467371 0.041931589 0.052277781 0.036881182254143746 0.028725515753126728 0.041827148 PE 18:1_22:2 0.023331402651533696 0.025661579243878577 0.032977017 0.040944709261188726 0.028418641810092737 0.029780302596187556 PE 18:1_22:3 0.035430743 0.040348716 0.052049705313496786 0.080193812 0.049940010868584135 0.048509534828245986 PE 18:1_22:4 0.027085683542910254 0.038357824 0.047007018 0.05697006 0.04138177 0.039140845 PE 18:1_22:6 0.086031131 0.11304642108637875 0.15516082970344763 0.088246633 0.070345188 0.06767299 PE 18:1_24:1 0.018435871263996734 0.020609689596040967 0.024269878238256402 0.015037431729547494 0.010744927804841469 0.020481736 PE 18:1_24:2 0.005629162 0.006572022 0.011034940855348915 0.006135159 0.004960658 0.005433392 PE 18:2_18:2 0.004641625 0.006479108 0.006827308 0.014377083646207267 0.006921869 0.00513886 PE 22:0_18:1 0.005882211 0.006871698 0.008906465 0.004127969 0.003532294 0.004800783 PE 24:0_18:1 0.008367496 0.00930802 0.010231725574201852 0.003053631 0.002656984 0.004329913 PE O-16:1_16:0 0.019651947165603045 0.021817844 0.024570598900801297 0.077072411 0.049725676 0.050448905 PE O-16:1_16:1 0.052414735 0.061283754774186495 0.08020275 0.15267429827036488 0.0948712 0.13002766424192228 PE O-16:1_18:1 0.4481083376801127 0.49924649070095706 0.6283355170056663 1.0743671842220806 0.5753043417456384 0.8953619735965129 PE O-16:1_20:1 0.26388608160663507 0.29950409756725677 0.3445999962921647 0.3958345096950947 0.28515515180476253 0.439523983 PE O-16:1_20:3 0.049952200439665084 0.048168505 0.061677161 0.099913559 0.054538938 0.058444468522746526 PE O-16:1_20:4 0.041270996 0.046730386 0.071678454 0.049277905 0.085331254 0.039012377774316175 PE O-16:1_20:5 0.022049141 0.026542339851318243 0.038872804666327594 0.030864180011137467 0.022867255185308046 0.031834365057988236 PE O-16:1_21:3 0.013756069024132539 0.018602402 0.020226095231055733 0.045895545 0.02761791 0.028549424043059178 PE O-16:1_22:1 0.075434452 0.079694662 0.089336682 0.13028791519338545 0.09243064 0.12796779016139787 PE O-16:1_22:3 0.21352400649786876 0.22621959107864392 0.2787674161511769 0.717265025 0.404356864 0.4041376227381519 PE O-16:1_22:4 0.1972996076568064 0.23671170881514214 0.3156453169435838 0.44712850460028025 0.31547793108111943 0.33827417965549056 PE O-16:1_22:5 0.075223482 0.096025179 0.12907414785382618 0.031192102684211326 0.032863682 0.04618495 PE O-16:1_22:6 0.5042842199757887 0.6470865276439097 0.8427879072380648 0.43701409937991126 0.39648213437010255 0.5885554956269801 PE O-16:1_24:6 0.071018654 0.096759379 0.12952282433935344 0.037589279658952056 0.040694693 0.06629355 PE O-17:1_20:4 0.008252814 0.008606672 0.014830497663169413 0.004469359 0.003781218 0.00500251 PE O-17:1_22:3 0.010433273 0.011246148719361585 0.013910513745772768 0.017691641047700924 0.013093897330664676 0.014827601224225218 PE O-18:1_16:0 0.018102878 0.017417122395316044 0.020439801 0.017565872919960736 0.015437223139882416 0.023749058083144974 PE O-18:1_20:4 0.1320888211367452 0.16447725765749854 0.2356500139442061 0.079281991 0.061124045 0.083508545 PE O-18:1_22:1 0.014006805574102676 0.013567654 0.014183475 0.016513960483878334 0.012156461 0.017976138 PE O-18:1_22:3 0.040607155917661945 0.045646175 0.053049818874485184 0.033729126 0.028063211760967488 0.038758325531730656 PE O-18:1_22:5 0.020889704244405324 0.026187464124277412 0.035820598 0.003845744 0.0045484 0.007152861 PE O-18:1_22:6 0.16350858156494952 0.20010987562403376 0.2883358259740917 0.048598511 0.046132020100244225 0.087088558 PE O-18:1_24:5 0.011698872474856622 0.015736601964417576 0.019847049496487284 0.004948498 0.003625199 0.005162216 PE O-18:1_24:6 0.019956863 0.025650515 0.031731215 0.004227387 0.005529528 0.010521844973801492 PE O-18:2_16:1 0.017785317 0.023973809040360068 0.029002131486440274 0.082041551 0.04980222 0.060718139 PE O-18:2_18:1 0.19601459766212856 0.21220247707295758 0.27241048664610956 0.5846193387433731 0.3774198250798989 0.4714441928809527 PE O-18:2_20:1 0.12000013027717098 0.13521980886861232 0.1559418187574697 0.3168628687786929 0.20617265441878663 0.27943174745023663 PE O-18:2_22:1 0.038386659075317735 0.039993865545435284 0.047735412742960295 0.093787868 0.060978814802594816 0.081323168 PE O-18:2_22:2 0.040595794 0.045616071 0.054411989 0.10223220718315035 0.065136122 0.07491753 PE O-18:2_22:3 0.11740430327919353 0.12500795140238863 0.1543375784403507 0.3130092521158231 0.21108303317898544 0.21368396646724228 PE O-18:2_22:4 0.081798586 0.10699293004000315 0.13795474689001153 0.187872262 0.1386074289980555 0.1585579335318329 PE O-18:2_22:5 0.064421332 0.08617018 0.1131707014547903 0.10633294718473767 0.095552323 0.12373839370774668 PE O-18:2_22:6 0.15859446576474065 0.210520093 0.2882004783450739 0.17820655988712447 0.17051654136817496 0.23715251155671704 PE O-18:2_24:5 0.018436267206094623 0.022968892863250448 0.029508811377887956 0.011833244109957059 0.010781018455294583 0.015063412449433846 PE O-18:2_24:6 0.018568164 0.024500863653575258 0.031628359 0.014635104546187341 0.015563022 0.025628362422234954 PE O-20:1_16:0 0.006145062 0.00709353 0.007607236 0.008355284 0.00618003 0.011699369134338063 PE O-20:1_20:4 0.010918288887361287 0.014247634922852269 0.017023961546423043 0.008451489 0.006539472 0.007707891 PE O-20:1_22:6 0.008966401 0.010889643721612262 0.013829188990139804 0.004098756 0.003697829 0.005833087 PG 14:0_18:1 0.003945509 0.002591902 0.003208253 0.009912179 0.009559405 0.007221131 PG 16:0_18:1 0.084732482 0.083784594 0.11662974522948295 0.14411697799135226 0.12562665414136723 0.14323370328558074 PG 17:0_19:0 0.033788396070252726 0.037136104821686486 0.053242907 0.024458356 0.023074579502411798 0.030802311144902436 PG 18:1_18:1 0.038959398 0.032155969683822505 0.041341146743436724 0.052821305 0.057683257 0.059306476168124324 PG 18:1_18:2 0.006189521 0.005342399 0.006120862 0.027001516778173893 0.019596545195232403 0.018257691384510562 PG 18:1_20:2 0.019650156344418006 0.014643282375789626 0.016059407 0.068114605 0.047743766 0.041206637 PG 18:1_20:3 0.004405965 0.001365073 0.001063049 0.018684085731861838 0.010822226638684212 0.008624648 PG 18:1_20:4 0.002255678 0.002060552 0.002061892 0.011633491412354783 0.008029348 0.005256016 PG 18:1_22:6 0.023440209 0.026895746 0.040248159 0.019240292 0.023918973304165748 0.033070195 PS 16:0_16:0 0.025878172 0.028930723 0.041372581 0.10005973053434554 0.062892696 0.048786342 PS 16:0_16:1 0.023495929740457226 0.022588651308881384 0.03275848 0.097991436 0.07826948 0.041704708 PS 16:0_18:1 0.3044110104306177 0.30356153500929595 0.4188706915928463 0.6457998547230014 0.492075178 0.4772078103283209 PS 16:1_18:1 0.03527441 0.033882930316938245 0.050788904 0.1582060744134062 0.12243845009235094 0.07520441 PS 18:0_18:1 1.0792810782171298 1.1198665116337259 1.5489265641564467 0.998712319 0.8824738168053805 1.1980543937982413 PS 18:0_20:3 0.015112623818183544 0.013121429524278158 0.15911811002483636 0.050562888 0.030163488553756366 0.1194015234751554 PS 18:0_20:4 0.082546665 0.098096144 0.1595473903299751 0.07604016 0.081873764 0.060711327262283565 PS 18:0_22:6 0.22523531177902367 0.25230569546824794 0.388948801 0.12332392148940115 0.1282125252237835 0.13423918685955363 PS 18:1_18:1 0.091503761 0.024414140047678733 0.45927808457114816 0.2744273124603472 0.1362658483307345 0.699397853 PS 18:1_18:2 0.026001926947881615 0.028935839333639152 0.037711215 0.070514104 0.045011831 0.038955606740218605 PS 18:1_20:1 0.013698668427796268 0.010408067684860552 0.077607912 0.043090086854893205 0.046458965398550385 0.11269422749260506 PS 18:1_20:3 0.020682974 0.025246329027601797 0.030114483693354385 0.034645623 0.021626853142936168 0.026985107 PS 40:6 0.004969202 0.002124218 0.013558653477503176 0.006021089 0.003091733 0.022158515 PS 40:7 0.022737103 0.026422097778423505 0.046098183 0.027427777822737885 0.0312239 0.020242063 PS O-18:0_18:1 0.056490308 0.053903477 0.074681139 0.024368355747202333 0.031343164 0.057267261 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Average Rt(min) Average Mz CL 16:0_18:1_18:1_18:1 21.051 1430.01123 CL 16:1_16:1_16:1_18:1 18.29 1371.93591 CL 16:1_18:1_16:1_18:1 19.319 1399.96362 CL 18:1_18:1_18:1_18:1 21.125 1456.02808 PC 14:0_14:0 8.645 722.4978 PC 14:0_16:0 9.932 750.52911 PC 14:0_16:1 8.845 748.51294 PC 14:0_20:4 8.895 798.53235 PC 16:0_16:0 11.205 778.56061 PC 16:0_16:1 10.09 776.54633 PC 16:0_17:1 8.602 790.56226 PC 16:0_18:0 12.487 806.59296 PC 16:0_18:1 11.332 804.57776 PC 16:0_19:1 11.927 818.59161 PC 16:0_20:4 10.18 826.56061 PC 16:0_22:6 9.874 850.56049 PC 16:1_16:1 7.402 774.52936 PC 16:1_18:1 10.259 802.55994 PC 16:1_18:2 7.689 800.54706 PC 16:1_20:4 9.065 824.54504 PC 16:1_22:6 8.791 848.54626 PC 17:0_18:1 10.133 818.59174 PC 17:0_19:0 13.653 834.6236 PC 17:1_18:1 8.73 816.57898 PC 18:0_18:1 12.57 832.6073 PC 18:0_20:4 11.447 854.59265 PC 18:0_22:5 11.49 880.60773 PC 18:0_22:6 11.11 878.59106 PC 18:1_16:2 9.836 800.54407 PC 18:1_18:1 11.465 830.59283 PC 18:1_18:2 10.552 828.57568 PC 18:1_19:1 12.042 844.60962 PC 18:1_20:1 12.64 858.62262 PC 18:1_20:2 11.676 856.60742 PC 18:1_20:3 10.868 854.59235 PC 18:1_20:4 10.291 852.57782 PC 18:1_22:2 12.843 884.6394 PC 18:1_22:3 12.104 882.62372 PC 18:1_22:4 9.824 880.60846 PC 18:1_22:5 10.346 878.591 PC 18:1_22:6 9.967 876.57843 PC 18:1_24:1 14.889 914.68732 PC 18:1_24:3 13.173 910.65723 PC 18:1_24:5 11.551 906.62262 PC 18:1_24:6 10.763 904.60706 PC 18:2_18:2 9.631 826.56024 PC 20:1_20:1 13.764 886.65558 PC 20:1_22:6 11.155 904.60791 PC 36:6 8.615 822.53162 PC 38:3 12.336 856.6076 PC 42:6 9.758 906.61798 PC O-16:0_14:0 10.738 736.5506 PC O-16:0_16:0 10.337 764.58087 PC O-16:0_16:1 10.96 762.56708 PC O-16:0_20:0 14.509 820.64441 PC O-16:0_20:1 13.339 818.62903 PC O-16:0_20:3 9.999 814.59668 PC O-16:0_20:4 8.88 812.58093 PC O-16:0_20:5 10.109 810.56744 PC O-16:0_21:3 12.197 828.61401 PC O-16:0_22:1 14.513 846.66211 PC O-16:0_22:2 13.477 844.64575 PC O-16:0_22:3 12.815 842.6297 PC O-16:0_22:4 11.91 840.61511 PC O-16:0_24:3 13.732 870.6593 PC O-16:0_24:5 12.276 866.62952 PC O-16:0_24:6 11.429 864.61353 PC O-16:1_14:0 10.537 734.53473 PC O-16:1_16:0 11.847 762.56641 PC O-16:1_16:1 10.752 760.55017 PC O-16:1_18:1 11.951 788.58191 PC O-16:1_18:2 10.915 786.565 PC O-16:1_20:1 13.155 816.61481 PC O-16:1_20:3 11.655 812.58099 PC O-16:1_20:4 10.728 810.56744 PC O-16:1_22:1 14.301 844.64539 PC O-16:1_22:4 12 838.59747 PC O-16:2_18:0 11.11 788.58221 PC O-18:0_16:0 13.309 792.61255 PC O-18:0_24:5 13.453 894.6604 PC O-18:1_16:0 12.138 790.59772 PC O-18:1_16:1 9.391 788.58209 PC O-18:1_18:1 12.263 816.61206 PC O-18:1_20:4 11.036 838.59778 PC O-18:1_22:1 14.587 872.67639 PC O-18:1_22:3 12.95 868.64624 PC O-18:1_22:4 11.96 866.63019 PC O-18:1_24:2 14.766 898.69232 PC O-18:1_24:3 13.877 896.6745 PC O-18:1_24:4 13.227 894.66077 PC O-18:1_24:5 12.337 892.64539 PC O-18:1_24:6 11.497 890.63031 PC O-18:2_16:1 9.156 786.56512 PC O-18:2_18:1 12.023 814.59875 PC O-18:2_22:1 14.396 870.65918 PC O-18:2_22:3 12.73 866.62939 PC O-18:2_22:4 11.885 864.61346 PC O-18:2_22:6 10.484 860.58081 PC O-19:1_16:0 12.752 804.61346 PC O-19:1_18:1 12.976 830.62805 PC O-20:1_18:1 11.99 844.64368 PC O-22:5_16:0 11.552 838.59772 PC O-24:1_16:0 15.764 874.69269 PC O-24:1_18:1 15.796 900.70886 PE 14:0_16:0 10.194 662.47693 PE 14:0_16:1 9.092 660.46057 PE 16:0_16:0 11.494 690.50848 PE 16:0_16:1 10.373 688.49402 PE 16:0_18:1 11.628 716.52496 PE 16:0_20:3 11.382 740.52344 PE 16:0_20:4 10.462 738.5097 PE 16:1_16:1 9.287 686.47644 PE 16:1_18:1 10.519 714.50897 PE 16:1_18:2 9.573 712.49347 PE 16:1_22:6 9.04 760.4942 PE 17:0_17:0 10.945 718.54016 PE 17:0_19:0 13.853 746.57178 PE 17:1_18:1 11.181 728.52405 PE 18:0_18:1 12.842 744.55536 PE 18:0_20:1 13.9 772.58612 PE 18:0_20:3 12.601 768.55585 PE 18:0_20:4 11.728 766.53888 PE 18:0_22:3 13.446 796.58875 PE 18:0_22:5 11.777 792.55554 PE 18:0_22:6 9.78 790.5412 PE 18:0_24:5 12.946 820.58807 PE 18:1_18:1 11.731 742.53949 PE 18:1_18:2 10.829 740.526 PE 18:1_19:1 12.319 756.55524 PE 18:1_20:1 12.881 770.57263 PE 18:1_20:2 12.023 768.55609 PE 18:1_20:3 11.17 766.53888 PE 18:1_20:4 10.587 764.52332 PE 18:1_22:1 13.989 798.60205 PE 18:1_22:2 13.103 796.58838 PE 18:1_22:3 12.357 794.5708 PE 18:1_22:4 11.486 792.55554 PE 18:1_22:6 10.327 788.52527 PE 18:1_24:1 15.087 826.63354 PE 18:1_24:2 14.114 824.61798 PE 18:2_18:2 9.874 738.50787 PE 22:0_18:1 15.149 800.61957 PE 24:0_18:1 16.352 828.64984 PE O-16:1_16:0 12.139 674.51379 PE O-16:1_16:1 11.022 672.49921 PE O-16:1_18:1 12.248 700.52856 PE O-16:1_20:1 13.382 728.5603 PE O-16:1_20:3 11.986 724.52917 PE O-16:1_20:4 9.789 722.51331 PE O-16:1_20:5 10.194 720.49774 PE O-16:1_21:3 12.29 738.54504 PE O-16:1_22:1 14.54 756.59143 PE O-16:1_22:3 12.867 752.56085 PE O-16:1_22:4 11.976 750.54541 PE O-16:1_22:5 11.551 748.53003 PE O-16:1_22:6 10.703 746.51428 PE O-16:1_24:6 11.518 774.54413 PE O-17:1_20:4 11.687 736.52911 PE O-17:1_22:3 13.208 766.57739 PE O-18:1_16:0 13.339 702.54559 PE O-18:1_20:4 12.308 750.54559 PE O-18:1_22:1 15.66 784.62341 PE O-18:1_22:3 14.006 780.59259 PE O-18:1_22:5 12.765 776.56085 PE O-18:1_22:6 11.968 774.54395 PE O-18:1_24:5 13.474 804.59363 PE O-18:1_24:6 12.71 802.57568 PE O-18:2_16:1 11.249 698.51251 PE O-18:2_18:1 12.346 726.54584 PE O-18:2_20:1 13.57 754.57617 PE O-18:2_22:1 14.583 782.60712 PE O-18:2_22:2 13.677 780.59277 PE O-18:2_22:3 12.867 778.57703 PE O-18:2_22:4 11.97 776.56091 PE O-18:2_22:5 11.212 774.54468 PE O-18:2_22:6 10.801 772.52911 PE O-18:2_24:5 12.408 802.57867 PE O-18:2_24:6 11.597 800.56213 PE O-20:1_16:0 12.123 730.57367 PE O-20:1_20:4 13.492 778.57764 PE O-20:1_22:6 13.145 802.57812 PG 14:0_18:1 9.027 719.48676 PG 16:0_18:1 10.198 747.51978 PG 17:0_19:0 12.337 777.56366 PG 18:1_18:1 9.518 773.53522 PG 18:1_18:2 8.78 771.51825 PG 18:1_20:2 9.779 799.55096 PG 18:1_20:3 9.129 797.53387 PG 18:1_20:4 8.671 795.5191 PG 18:1_22:6 8.313 819.51965 PS 16:0_16:0 10.052 734.49976 PS 16:0_16:1 9.045 732.48193 PS 16:0_18:1 10.187 760.51447 PS 16:1_18:1 9.151 758.49982 PS 18:0_18:1 11.397 788.54651 PS 18:0_20:3 8.038 812.54437 PS 18:0_20:4 10.299 810.53015 PS 18:0_22:6 9.985 834.5293 PS 18:1_18:1 7.363 786.53009 PS 18:1_18:2 9.382 784.51483 PS 18:1_20:1 8.883 814.56189 PS 18:1_20:3 9.783 810.53058 PS 40:6 6.505 834.52954 PS 40:7 8.923 832.51447 PS O-18:0_18:1 12.213 774.56677 METABOLITES_END #END