#METABOLOMICS WORKBENCH shancock_20250219_170319 DATATRACK_ID:5649 STUDY_ID:ST003758 ANALYSIS_ID:AN006171 PROJECT_ID:PR002341 VERSION 1 CREATED_ON February 20, 2025, 2:22 pm #PROJECT PR:PROJECT_TITLE Untargeted lipidomics of gemcitabine-resistant PDAC cells PR:PROJECT_SUMMARY Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive disease with few PR:PROJECT_SUMMARY treatment options and poor survivability. In this work we sought to characterise PR:PROJECT_SUMMARY metabolic adaptations to gemcitabine (GEMC)-based chemotherapy exposure to PR:PROJECT_SUMMARY discover new therapeutic targets for improving treatment efficacy. We show that PR:PROJECT_SUMMARY GEMC resistance (GEMR) upregulates de novo lipogenesis in Panc1 and MiaPaCa2 PR:PROJECT_SUMMARY cells. PR:INSTITUTE Victor Chang Cardiac Research Institute PR:LAST_NAME Hancock PR:FIRST_NAME Sarah PR:ADDRESS Lowy Packer Building, 405 Liverpool Street, Darlinghurst, NSW, 20210 Australia PR:EMAIL s.hancock@victorchang.edu.au PR:PHONE +61414537526 #STUDY ST:STUDY_TITLE Untargeted lipidomics of gemcitabine-resistant cells ST:STUDY_SUMMARY In this work we developed gemcitabine (GEMC) resistant (GEMR) PDAC cell lines ST:STUDY_SUMMARY from Panc1 and MiaPaCa2 cells. This dataset contains results and raw data for ST:STUDY_SUMMARY untargeted lipidomics of Panc1 cells, including control (CON), and GEMR cells ST:STUDY_SUMMARY alongside internal quality controls (iQC) and blank samples for background ST:STUDY_SUMMARY subtraction (as described under sample prep). Untargeted lipidomics enabled the ST:STUDY_SUMMARY quantification of more than 400 distinct molecular lipid species within Panc1 & ST:STUDY_SUMMARY MiaPaCa2 control (CON) & GEMR cells from 12 different classes of lipid, with 79 ST:STUDY_SUMMARY molecular species being differentially abundant between MiaPaCa2 control and ST:STUDY_SUMMARY GEMR cells following unpaired t test. Data were analysed using MS-DIAL 4.9. ST:INSTITUTE Victor Chang Cardiac Research Institute ST:LAST_NAME Hancock ST:FIRST_NAME Sarah ST:ADDRESS Lowy Packer Building, 405 Liverpool Street, Darlinghurst, NSW, 2010, Australia ST:EMAIL s.hancock@victorchang.edu.au ST:PHONE +61414537526 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - Blank_cells_neg Sample source:Blank | group:Blank RAW_FILE_NAME(Raw file name)=Blank_cells_neg.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - Blank_cells_neg_20211210204022 Sample source:Blank | group:Blank RAW_FILE_NAME(Raw file name)=Blank_cells_neg_20211210204022.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - Blank_cells_pos Sample source:Blank | group:Blank RAW_FILE_NAME(Raw file name)=Blank_cells_pos.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - Blank_cells_pos_20211210152840 Sample source:Blank | group:Blank RAW_FILE_NAME(Raw file name)=Blank_cells_pos_20211210152840.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - iQC_cells_neg Sample source:pool | group:iQC RAW_FILE_NAME(Raw file name)=iQC_cells_neg.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - iQC_cells_neg_20211210211133 Sample source:pool | group:iQC RAW_FILE_NAME(Raw file name)=iQC_cells_neg_20211210211133.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - iQC_cells_pos Sample source:pool | group:iQC RAW_FILE_NAME(Raw file name)=iQC_cells_pos.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - iQC_cells_pos_20211210155952 Sample source:pool | group:iQC RAW_FILE_NAME(Raw file name)=iQC_cells_pos_20211210155952.mzML; protein amount=- SUBJECT_SAMPLE_FACTORS - MiaPaCa2_CON_1_neg Sample source:MiaPaCa2 cells | group:CON RAW_FILE_NAME(Raw file name)=MiaPaCa2_CON_1_neg.mzML; protein amount=277.5669498 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_CON_1_pos Sample source:MiaPaCa2 cells | group:CON RAW_FILE_NAME(Raw file name)=MiaPaCa2_CON_1_pos.mzML; protein amount=277.5669498 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_CON_2_neg Sample source:MiaPaCa2 cells | group:CON RAW_FILE_NAME(Raw file name)=MiaPaCa2_CON_2_neg.mzML; protein amount=222.8989234 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_CON_2_pos Sample source:MiaPaCa2 cells | group:CON RAW_FILE_NAME(Raw file name)=MiaPaCa2_CON_2_pos.mzML; protein amount=222.8989234 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_CON_3_neg Sample source:MiaPaCa2 cells | group:CON RAW_FILE_NAME(Raw file name)=MiaPaCa2_CON_3_neg.mzML; protein amount=252.7464251 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_CON_3_pos Sample source:MiaPaCa2 cells | group:CON RAW_FILE_NAME(Raw file name)=MiaPaCa2_CON_3_pos.mzML; protein amount=252.7464251 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_GEMR_1_neg Sample source:MiaPaCa2 cells | group:GEMR RAW_FILE_NAME(Raw file name)=MiaPaCa2_GEMR_1_neg.mzML; protein amount=239.1103887 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_GEMR_1_pos Sample source:MiaPaCa2 cells | group:GEMR RAW_FILE_NAME(Raw file name)=MiaPaCa2_GEMR_1_pos.mzML; protein amount=239.1103887 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_GEMR_2_neg Sample source:MiaPaCa2 cells | group:GEMR RAW_FILE_NAME(Raw file name)=MiaPaCa2_GEMR_2_neg.mzML; protein amount=244.7938146 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_GEMR_2_pos Sample source:MiaPaCa2 cells | group:GEMR RAW_FILE_NAME(Raw file name)=MiaPaCa2_GEMR_2_pos.mzML; protein amount=244.7938146 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_GEMR_3_neg Sample source:MiaPaCa2 cells | group:GEMR RAW_FILE_NAME(Raw file name)=MiaPaCa2_GEMR_3_neg.mzML; protein amount=250.0487649 SUBJECT_SAMPLE_FACTORS - MiaPaCa2_GEMR_3_pos Sample source:MiaPaCa2 cells | group:GEMR RAW_FILE_NAME(Raw file name)=MiaPaCa2_GEMR_3_pos.mzML; protein amount=250.0487649 #COLLECTION CO:COLLECTION_SUMMARY Samples were obtained from established immortalised adherent human pancreatic CO:COLLECTION_SUMMARY ductal adenocarcinoma cells (MiaPaCa2). Cells were cultured in high glucose DMEM CO:COLLECTION_SUMMARY containing 4.5 g/L glucose and 4 mM glutamine and were supplemented with 10% CO:COLLECTION_SUMMARY FCS. Cells were cultured under 5% CO2 at 37ºC and routinely passaged every 2-3 CO:COLLECTION_SUMMARY days (when ~80-90% confluent). Cell lines were regularly screened for mycoplasma CO:COLLECTION_SUMMARY infection. CO:SAMPLE_TYPE Pancreas #TREATMENT TR:TREATMENT_SUMMARY MiaPaCa2 GEMR cells were produced by serial treatment with escalating doses of TR:TREATMENT_SUMMARY gemcitabine for approximately 12 weeks. The final concentration of GEMC for TR:TREATMENT_SUMMARY Panc1 cells was 64 nM, resulting in a half-maximal inhibitory concentration of TR:TREATMENT_SUMMARY 92.2 nM (approximately 3x increase over control (CON) cells, 30.6 nM) #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Lipids were extracted from cells grown to 80-90% confluency in 6 well plates SP:SAMPLEPREP_SUMMARY using a modified methyl tert-butyl ether (MTBE) method. Adherent cells were SP:SAMPLEPREP_SUMMARY washed with ice-cold PBS and scraped into methanol containing 0.01% butylated SP:SAMPLEPREP_SUMMARY hydroxytoluene and 100 pmol each of internal standards (phosphatidylcholine SP:SAMPLEPREP_SUMMARY 19:0/19:0, phosphatidylethanolamine 17:0/17:0, phosphatidylserine 17:0/17:0, SP:SAMPLEPREP_SUMMARY lysophosphatidylcholine 17:0, lysophosphatidylethanolamine 14:0, SP:SAMPLEPREP_SUMMARY phosphatidylglycerol 17:0/17:0, phosphatidic acid 17:0/17:0, ceramide SP:SAMPLEPREP_SUMMARY d18:1/17:0, dihydrosphingomyelin 12:0, cholesteryl ester 22:1, diacylglycerol SP:SAMPLEPREP_SUMMARY 17:0/17:0, D5-triacylglycerol 16:0/16:0/16:0 and cardiolipin SP:SAMPLEPREP_SUMMARY 14:0/14:0/14:0/14:0). An empty well was also scraped for use in background SP:SAMPLEPREP_SUMMARY subtraction. To this, MTBE was added at a ratio of 3:1 v/v, and extracts were SP:SAMPLEPREP_SUMMARY rotated overnight at 4ºC. Following overnight rotation, 1 part of ice-cold 150 SP:SAMPLEPREP_SUMMARY mM ammonium acetate was added, samples were vortexed vigorously and centrifuged SP:SAMPLEPREP_SUMMARY at 2000 x g for 5 min. The upper organic phase containing lipids was transferred SP:SAMPLEPREP_SUMMARY into a 1.5 mL autosampler vial and dried under N2 at 37ºC Dried lipids were SP:SAMPLEPREP_SUMMARY reconstituted in chloroform:methanol:water (60:30:4.5 v/v/v), transferred to a SP:SAMPLEPREP_SUMMARY sleeved vial, and stored at -20ºC until analysis. The aqueous phase containing SP:SAMPLEPREP_SUMMARY the protein pellet was dried under N2 and digested with 1M NaOH overnight at SP:SAMPLEPREP_SUMMARY 4ºC. Digested protein was diluted 1:2 v/v with water and protein concentration SP:SAMPLEPREP_SUMMARY was determined using a Pierce BCA assay kit (ThermoFisher Scientific) #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Thermo Dionex Ultimate 3000 CH:COLUMN_NAME Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) CH:SOLVENT_A Acetonitrile:water (3:2 v/v), 10 mM Ammonium Formate, 0.1% Formic acid CH:SOLVENT_B Acetonitrile:isopropanol (1:9 v/v), 10 mM Ammonium Formate, 0.1% Formic acid CH:FLOW_GRADIENT As described by Hu et al. 2008 J Proteome Research CH:FLOW_RATE 0.26 ml/min CH:COLUMN_TEMPERATURE 60 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME Thermo Q Exactive HF hybrid Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS Source conditions were as follows: a spray voltage 4.0 kV in positive ion mode MS:MS_COMMENTS and 3.5 kV in negative ion mode, capillary temperature 290°C, S lens RF 50, and MS:MS_COMMENTS auxiliary gas heater temperature of 250°C. Nitrogen was used as both source and MS:MS_COMMENTS collision gas, with the sheath gas flow rate set at 20 and the auxiliary gas MS:MS_COMMENTS flow rate at 5 (arbitrary units). Data were acquired in full scan/data-dependent MS:MS_COMMENTS MS2 mode (full-scan resolution 70,000 FWHM, max ion injection time 50 ms, scan MS:MS_COMMENTS range m/z 200–1,500), with the 10 most abundant ions being subjected to MS:MS_COMMENTS collision-induced dissociation using an isolation window of 1.5 Da and a MS:MS_COMMENTS normalized stepped collision energy of 15/27 eV. Product ions were detected at a MS:MS_COMMENTS resolution of 17,500. An exclusion list for background ions was developed using MS:MS_COMMENTS extraction blanks, and mass calibration was performed in both positive and MS:MS_COMMENTS negative ionization modes before analysis to ensure mass accuracy of 5 ppm in MS:MS_COMMENTS full-scan mode. #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS nmol/mg protein MS_METABOLITE_DATA_START Samples MiaPaCa2_CON_1_neg MiaPaCa2_CON_2_neg MiaPaCa2_CON_3_neg MiaPaCa2_GEMR_1_neg MiaPaCa2_GEMR_2_neg MiaPaCa2_GEMR_3_neg Factors Sample source:MiaPaCa2 cells | group:CON Sample source:MiaPaCa2 cells | group:CON Sample source:MiaPaCa2 cells | group:CON Sample source:MiaPaCa2 cells | group:GEMR Sample source:MiaPaCa2 cells | group:GEMR Sample source:MiaPaCa2 cells | group:GEMR CL 14:0_16:1_18:1_18:1 0.030101215 0.047251793 0.1258101481265607 0.013422084 0.018943395480701376 0.011647200629179148 CL 16:0_16:1_18:1_18:1 0.022081136 0.11624367176871735 0.14759122160742266 0.069205257 0.013430193253120096 0.026716753 CL 16:0_18:0_18:0_22:0 2.570812552681772 2.9777736623934974 2.679161088 1.6312244508972806 2.8435273141059683 2.461053335717892 CL 16:0_18:1_16:0_18:1 0.061560451 0.5845158108049031 0.3517021664762411 0.4586421633305112 0 0.21362019379253502 CL 16:0_18:1_16:1_18:1 0.22787317396672688 0.7802937662908693 0.6412037784871484 0.5651762842091174 0.3185655996970627 0.4216647352942881 CL 16:0_18:1_18:1_18:1 0.13839366883590495 0.42431062880540993 0.34056636916239896 0.3725776980594998 0.2652727901940334 0.3497704998710927 CL 16:1_16:1_16:1_18:1 0.2741624495206226 0.23284626805771871 0.4100692966833635 0.11007519126298433 0.11384554389888603 0.1123120767966073 CL 16:1_16:1_18:1_18:1 0.40747968218614716 0.4308682915752103 0.891293677 0.24602528147091782 0.2769184347606196 0.2783419727843072 CL 16:1_18:1_16:1_18:1 2.4453545746462217 2.4091660205814756 2.774073688847929 1.7323319447000218 1.9172152756680516 1.9560742011159202 CL 16:1_18:1_16:1_18:2 0.8161287172288616 0.6411158018630743 0.6807526567327847 0.44019025043923166 0.4977552853213692 0.4346670586857075 CL 16:1_18:1_18:1_18:1 1.5778773900194796 1.7541049561660038 1.9762378026516405 1.7225697886139506 2.0766530047941907 2.159100305162893 CL 16:1_18:1_18:1_18:2 1.181029996907925 1.1967254308045325 1.268450390518954 0.9499184047993763 0 1.1034422435683604 CL 18:1_18:1_18:1_18:1 0.2818149353007676 0.26257139076974667 0.3428949236042463 0.3912764828556911 0.6494491740616605 0.6073676751726069 CL 18:1_18:1_18:1_18:2 0.3782808386255035 0.35847094369832866 0.413982497 0.4389259728589989 0.6810072375925078 0.6432675572021621 CL 24:0_18:1_18:1_18:1 0 0 0 0 0.7397588714362733 0 PC 14:0_14:0 0.8576625266767975 1.137585255483129 0.8788553726978442 0.3075994456054279 0.39368067403796986 0.38111947824470915 PC 14:0_16:0 2.0905718112374476 2.764297456470037 2.2909538437032704 1.882378916235177 2.0177509051192546 1.9522154793416868 PC 14:0_16:1 1.2117775287680177 2.1324520632505064 1.4582616183163046 0.7158161127677972 0.7767557597837206 0.7123069289130934 PC 15:0_16:1 0.12210791768784615 0.20613541370761815 0.15208661128971976 0.10772008295254813 0.093811574 0.094901714 PC 16:0_16:0 2.3745575560261565 3.7322583353266765 2.842459967318649 3.3985888207019563 3.312081473161452 3.326458715150754 PC 16:0_16:1 11.318412506476685 20.825234401528384 14.391222184443128 11.691636392078172 10.438611430179042 10.16475825563964 PC 16:0_17:1 1.1244528055993548 1.6881512705698014 1.3474688614842558 1.2494481981732772 1.0616657814289352 1.0294158304365706 PC 16:0_18:0 0.4911128389304452 0.694191682 0.5705386400841882 0.7879683949687347 0.8127866843163891 0.7651215335536075 PC 16:0_18:1 25.398960307498918 39.28688933373393 31.95395110435594 36.24073075252449 31.559362611193258 32.02722649945556 PC 16:0_20:4 0.39789278891051266 0.6206026487431153 0.4547513798291822 0.47002228044520966 0.40890318987497737 0.3560800434596724 PC 16:0_20:5 0.042456525 0.065989203 0.041603063253453997 0.034249484 0.031833862 0.026565127 PC 16:0_22:6 0.14201245042093324 0.24665035185491868 0.17552831669993121 0.2074403868028205 0.14724983963968674 0.138315399 PC 16:0_24:1 0.094618294 0.1280039494458393 0.11030170399798388 0.1398645132404841 0.1176910600355599 0.11036058499272539 PC 16:0_26:1 0.16731022938562634 0.22816412447126763 0.18249270372867266 0.1936351198140589 0.15986161239449423 0.14919942763797903 PC 16:1_16:1 0.8569854674445846 1.789497291929427 1.1156076937898274 0.8649798036634438 0.8244952309123432 0.8069680429669632 PC 16:1_17:1 0.12167579659250673 0.17804776246001017 0.1466685359200286 0.17137729296419985 0.11814646362504963 0.12970270539274834 PC 16:1_18:1 8.559391325823503 14.262100493656716 11.131578648009077 10.422734672125813 9.614863207 9.397923535545933 PC 16:1_18:2 0.2127269204744461 0.3566634539342041 0.2963475810901211 0.24235468282661538 0.2160056489720776 0.2190750258667883 PC 16:1_20:4 0.062611061 0.1043240629290618 0.078410726 0.066231544 0.057416649482935546 0.052906936765980565 PC 16:1_22:6 0.042184167 0.078892463 0.05579591 0.040551325 0.041913524959506794 0.038509122 PC 17:0_18:1 0.7038842781348947 0.9018180870877163 0.8008379324317251 1.0648673888094675 0.9382439139451257 0.9401237699601844 PC 17:1_18:1 0.7871248547154782 1.2306109909423966 0.9974939617493837 0.9824740389660157 1.031257286573869 0.9896044459448342 PC 18:0_18:1 7.644295296227665 7.223341910763501 6.669324490752083 8.016980503569071 11.255269932540157 9.183741393616149 PC 18:0_20:4 0.1778829702314771 0.2437283481315078 0.1843886183905238 0.18661035171697207 0.23125471686981927 0.19062754517606015 PC 18:0_22:5 0.13792237722372086 0.17110058572833575 0.14597241460931437 0.14216925768235664 0.15366758612625095 0.16353399573160748 PC 18:0_22:6 0.11259438000061243 0.13735438561956187 0.1343722665250211 0.11555372692062496 0.15054320735850363 0.13335857243013477 PC 18:1_18:1 23.596689711598497 29.006420146324707 25.986445948495493 27.695588561056756 31.19062194634876 32.979500877289176 PC 18:1_18:2 1.924025624979297 2.6956559468503585 2.351597211642791 2.0607130228076715 2.285081490260571 2.202896548650022 PC 18:1_19:1 0.5468716970412212 0.7139172459563002 0.6459488758367665 0.7952447441742275 0.6328043418762681 0.6206155358413916 PC 18:1_20:1 2.961515305048717 3.512692108487565 3.583131818237983 4.295275155927559 3.8814740208779446 3.658275443729943 PC 18:1_20:2 0.3489608737896733 0.41911353965580794 0.42019412935215944 0.4453835593533377 0.40946061057432576 0.41256621451867265 PC 18:1_20:3 0.37753836589491935 0.5852797625829242 0.4836971879057596 0.4845107210915937 0.4332553741639923 0.4151594647912636 PC 18:1_20:4 0.6064062201386761 0.9596826213408015 0.7414966626415506 0.8266156892889365 0.8273118202629736 0.7579703419339148 PC 18:1_21:1 0.078735651 0.095523968 0.092502403 0.1043824982355246 0.073342758 0.070087967 PC 18:1_22:1 0.35617710030157596 0.3876106879603942 0.43380966666516335 0.4761879271222936 0.4982182327146917 0.4509474954451066 PC 18:1_22:2 0.089000964 0.097262233 0.10330969492865946 0.14455993737075565 0.1844432488926571 0.16043635511416965 PC 18:1_22:4 0.081694796 0.14069503571863728 0.10700148836196247 0.15912132036540907 0.16297720210964015 0.12669902523429602 PC 18:1_22:5 0.17888364147476213 0.3162463394293702 0.24327402891103095 0.2929590859039003 0.292252911 0.2933840596928759 PC 18:1_22:6 0.249993971 0.3654509644728523 0.32169008313797665 0.3156652900617073 0.35619641247626166 0.36180476999723593 PC 18:1_24:1 0.3177251964061331 0.3871988946979341 0.3373218637067879 0.41441659 0.3675518437835485 0.35325633345966423 PC 18:1_24:2 0.061811085820705006 0.070547069 0.062228582 0.096063279 0.10012469000537466 0.095199744 PC 18:1_26:1 0.32085012609416214 0.3754730360641176 0.35321479811383827 0.3348780354960995 0.2566004955429623 0.2743725583765141 PC 18:1_26:2 0.063428614 0.084780713 0.07512443 0.087935854 0.10327065310847268 0.099880518 PC 18:1_28:1 0.065405377 0.079147484 0.065929107 0.050903198964503744 0.033526723 0.041294313442370306 PC 18:2_18:2 0.10031765922732316 0.18382995543219824 0.12161509017780311 0.12458724329730374 0.11014454279624626 0.10108920206240718 PC 19:0_18:1 0.10882771665006706 0.11156513511727255 0.10890955060326703 0.1429899168617032 0.1366031782433051 0.12220815999828792 PC 26:0_18:1 0.1265297 0.15175586946003755 0.1326390631451458 0.10477542468061823 0.10964020156464212 0.10922260446071917 PC 34:0 0 0 0 0.1291785611507458 0.077567494 0.055213241 PC 40:4 0.063343769 0.10087953964732387 0.077735548 0.10730753125109134 0.08549093 0.064640046 PC O-16:0_14:0 1.545058531314315 1.857603894837043 1.644144924 1.219442431556606 0.9317805950497714 0.9254667918298076 PC O-16:0_16:0 3.454694987732917 5.0408783745219745 4.394888907744242 4.085000265079141 2.4861558824908245 2.7082177163156445 PC O-16:0_16:1 5.0499473231029395 7.862159771 6.580719000991577 3.692130684301737 2.371183931 2.570284705835378 PC O-16:0_18:1 9.565612003303272 14.030653030519808 12.658517505405797 11.795776319970505 7.7295278479785585 7.729498064642671 PC O-16:0_20:4 0.6730242434961066 1.1964918268254028 0.771066446 0.8484927399562979 0.33193601400165074 0.35312829029564446 PC O-16:0_20:5 0.088981609 0.13989070462241088 0.09994154 0.074365723 0.035030476 0.035424149 PC O-16:0_22:4 0.067235199 0.11860820994854417 0.093391394 0.10114038957525397 0.065534246 0.059556781340023776 PC O-16:1_14:0 0.13323280144415078 0.1295022037392776 0.1375388205470355 0.074249589 0.080141281 0.070030402 PC O-16:1_16:0 0.3249872823172506 0.33203181857853087 0.3976860285189759 0.29549248360966046 0.32581903062086714 0.2607845980461451 PC O-16:1_16:1 0.2123188675835206 0.26771726153618897 0.26987161937788173 0.14583773998710436 0.14972262048245497 0.14165074296148575 PC O-16:1_18:1 0.4871098560308137 0.5599546588826854 0.6171502663410161 0.42108379525156064 0.3395047565731387 0.3406468106661052 PC O-16:2_18:0 1.345990348765459 1.8675658955554857 1.6320826966094422 1.1920922176347475 0.7283713965430433 0.7982679355845178 PC O-18:0_16:0 0.6429129832521087 0.8232532089010227 0.7618203151208497 0.8255657652553031 0.7642483832231365 0.8014297325426015 PC O-18:0_18:1 1.5607820700691477 1.9805314006767103 2.0651452042848444 1.968511937505835 1.9889095981906004 1.9394739604641174 PC O-18:0_20:4 0.1469073081933228 0.20668969714241262 0.18225864931741992 0.20613002039544262 0.17791226785182013 0.16479301197855878 PC O-18:0_22:5 0.060311707 0.091386083 0.074340267 0.090853942 0.074404717 0.062790697 PC O-18:0_22:6 0.064733585 0.097181876 0.07831041 0.096293169 0.08029602 0.073998224 PC O-18:1_18:1 1.972840965720137 2.5475408847506973 2.557572231682135 2.379343901123857 2.163755583775249 2.1134020807022895 PC O-18:1_22:4 0.048513860411987686 0.1004454737669494 0.068875195 0.09146715 0.052975207 0.028620129 PC O-18:1_22:6 0.10261717293064437 0.17051564522517998 0.13743272998691916 0.16604251223067903 0.12012001888394108 0.11257002021007471 PC O-18:2_18:1 0.18720042972642986 0.2513653392248224 0.23268590293660651 0.17200565453116254 0.13319821544286053 0.11824542508684409 PC O-20:0_18:1 0.2925745824879691 0.32213340626004267 0.319243181 0.2459762221705055 0.2484908995746453 0.22016479649674778 PC O-20:1_18:1 0.24410607074406818 0.3139321098119599 0.28084229850504105 0.2577960628602286 0.20326792 0.20187124427371225 PC O-22:0_16:0 0.050902944 0.040864916935127005 0.047968025233531275 0.024631491 0.029613449237068216 0.027706442951092054 PC O-22:1_18:1 0.21226713477633208 0.2041090906535678 0.2125983953229467 0.10467657808122126 0.095056439 0.1006800299238156 PC O-24:1_16:0 0.32065834655554315 0.2993612994930176 0.304821467 0.16161027583549922 0.18182367977726965 0.17105278645067043 PC O-24:1_18:1 0.30458770215774367 0.24814889422169964 0.26553117324444636 0.11357467475263516 0.13252923664247057 0.1178548945595727 PC O-26:1_16:0 0.085186186 0.077636251 0.08592164 0.030804313 0.036580899807372835 0.032206348 PC O-26:1_18:1 0.044958725 0.030953313889895806 0.044057448 0.015670359479047085 0.017387531916382825 0.014587151 PE 14:0_16:1 0.081611663 0.11251156101637579 0.087423189 0.028435051127309523 0.035270562 0.032756544512889715 PE 16:0_16:0 0.12055780922064929 0.18309399544859276 0.14118588090066497 0.14656880722336968 0.11394697193611734 0.11004264498663949 PE 16:0_16:1 2.3779550135414436 3.421057553704408 2.9476332182760174 1.4323438879377866 1.2178480887266798 1.208087584224298 PE 16:0_18:1 9.721875449512277 13.009672276285263 10.837845957804761 6.773427013505091 7.032285829132217 6.883630581 PE 16:0_20:4 0.825449422 1.2079591076902416 0.913616811 0.4266706846477301 0.49614029747433497 0.46785598376925985 PE 16:1_16:1 0.4948418249094144 0.495647992 0.4533263816430869 0.16613505658643554 0.18330487934968812 0.18106935748247246 PE 16:1_18:1 7.005545985733197 6.854754563946713 6.588242787837041 3.3573590329447334 4.523996949408436 4.1560448242381405 PE 16:1_18:2 0.1620766939851991 0.1421724822287757 0.1263162568604674 0.055474072 0.081033163 0.074409271 PE 16:1_20:4 0.1606961593179946 0.1386412886944542 0.13257837303071213 0.046648155 0.080597663 0.067586179 PE 17:0_18:1 0.4761504741519883 0.4855706069403144 0.418519245 0.34596212637368445 0.3852895067178875 0.3769456137842562 PE 17:1_18:1 0.5976465968370052 0.5170765183292142 0.4639284983924341 0.3290390516507234 0.4989896380817266 0.5086368982069615 PE 18:0_18:1 11.23072403 11.398171775119328 10.912201869625195 10.014194242473446 13.27660257487508 12.812807763902322 PE 18:0_20:1 0.3348843832317999 0.2913181650972011 0.32003031134908316 0.32116581068803063 0.4560502487670884 0.3939042879921528 PE 18:0_20:3 0.13517509729918223 0.14330880385142944 0.12706097725747612 0.075469636 0.079997277 0.084969468 PE 18:0_20:4 2.9420880546585764 3.7444569332793276 3.312994516544936 2.2781015190143736 2.610151462521646 2.570387461999977 PE 18:0_20:5 0.3040775024046988 0.2758662159015951 0.2489288668120861 0.12841064279530956 0.21821209666060437 0.2362769650477671 PE 18:0_22:4 0.24107696287246494 0.3552798187417966 0.2898335893387045 0.3156784142808914 0.3257021806768609 0.30947934049257586 PE 18:0_22:5 1.0992849231138853 1.3785570443887312 1.0995194582148802 1.0023294990682758 1.0174006519538807 0.9796022999631498 PE 18:0_22:6 0 0.089514495 0 0 0 0 PE 18:1_18:1 20.913270959837096 16.02675394489855 17.77751430220355 13.058427014282149 25.66537089 23.867199672678517 PE 18:1_18:2 1.7989469307075594 1.3798554662577656 1.4812094317172446 0.87567067932568865 1.5408962842883314 1.3533218112144365 PE 18:1_19:1 0.40109770896325625 0.3059320379792452 0.36977709767179606 0.3377326058429636 0.46363050807192197 0.36358880023380674 PE 18:1_20:1 0.10144304166185955 0.12186415249714978 0.11554254168872856 0.15071941300130365 0.12125027742709199 0.096141244 PE 18:1_20:3 0.8273693898232347 0.8848657415715829 0.8230568971599186 0.5698987566457193 0.44959359870853555 0.6226336422590429 PE 18:1_20:4 2.4224812442383588 2.2275692892670507 2.2014674035040045 1.2317310535160757 2.413458238 2.031426136313049 PE 18:1_20:5 0.28875241366395343 0.23088050748563996 0.2582007416233782 0.089179642 0.2097601113414958 0.17909460418747447 PE 18:1_21:1 0.059736736 0.053080357 0.067671258 0.073904617 0.067380935 0.063409791 PE 18:1_22:1 0.6077690223499992 0.6146792377662934 0.6605979605970114 0.692281044 0.7640900142393471 0.7421865236194087 PE 18:1_22:2 0.082219174 0.081883377 0.090582306 0.10777445415190767 0.1287843202797997 0.1360420641281887 PE 18:1_22:5 0 0 0 0 0.272744787 0 PE 18:1_22:6 0.9424736110791776 0.8228801978008495 0.8358509952483965 0.49994075517334496 0.9533104448513008 0.770837194 PE 18:1_24:1 0.5910168303215686 0.6009126451636516 0.6378258978904844 0.6001286684761565 0.6036645026952104 0.6258753057039603 PE 18:1_24:2 0.074181544 0.072349044 0.08033577 0.094360695 0.11134070179927358 0.10843759711183014 PE 18:1_26:1 0.2530770620800813 0.3030795080701302 0.3113911952477532 0.2526762850731305 0.20304024395961545 0.20005224327069215 PE 22:0_18:1 0.36500128333976795 0.35364570983063165 0.3570158416781852 0.2722594781915149 0.2978236936561394 0.2915307425967933 PE 22:0_20:4 0.036668757 0.041599772 0.038280812801609984 0.032502269 0.036168168 0.032419554906861346 PE 22:1_20:4 0.079215641 0.094014424 0.088152951 0.068651592 0.064482512 0.063492288 PE 24:0_18:1 0.5666047544527284 0.5211201886815283 0.5492822087039664 0.47322301251560517 0.5711428202274023 0.5204197634129276 PE 24:0_20:4 0.075966991 0.072581315 0.059704918 0.045768845550568454 0.047363241 0.047065305 PE 24:1_20:4 0.1357439522513704 0.13604492356260875 0.14117099837437322 0.10043554295946906 0.083482159 0.078137804 PE 26:0_18:1 0.09764297 0.11323394347932415 0.10576747906679623 0.074706297 0.080707388 0.080551908 PE 26:1_20:4 0.049380121500257244 0.069522082 0.062805632 0.034143164 0.023015269863822956 0.025901516928339818 PE O-16:0_16:0 0.13012815295864733 0.096701743 0.12010203287308992 0.1275060714955073 0.1357308932742441 0.11440216377161236 PE O-16:0_16:1 2.217824955184434 1.3981751871299184 1.558080772289312 0.6149078718501831 0.8164082273524101 0.7714173623104024 PE O-16:0_17:1 0.24706815235072804 0.13084354157102787 0.16246920352577382 0.073927511 0.11829067621042103 0.10257990582723313 PE O-16:0_18:1 11.329728027384691 7.3115341901179285 8.983546718205695 6.179747555498368 9.768028288195845 8.628814978892711 PE O-16:0_20:3 0.6295966830893903 0.43784550376335424 0.5246140177039982 0.2892409602402116 0.4197688828942753 0.3620422952741489 PE O-16:0_20:4 3.769305862812148 3.747363030269634 3.6802832009823745 3.096724263438988 2.521826328 2.5460482338789445 PE O-16:0_20:5 0.7883397766458885 0.8638427791162463 0.8964965130102754 0.507346661 0.4863156740126347 0.47492870806609255 PE O-16:0_22:3 0.44327853607496126 0.33679394787829336 0.393940319 0.30306995679218063 0.4264970754219323 0.41923348438821156 PE O-16:0_22:4 1.558603401800044 1.493563616465118 1.413762249083735 1.7908601340470982 1.4273765945126689 1.6961270962944965 PE O-16:0_22:5 3.4627597749080166 3.4455274381828316 3.2582899448567457 2.737203370375349 2.819037457342722 2.7295036275848887 PE O-16:0_22:6 2.751847027866959 3.479797116434282 3.1456294950200667 2.6736021437646547 2.0448609343416715 2.113542094 PE O-16:1_16:1 0.8159183193234435 0.6729961912006401 0.7805020116950615 0.2486672441149353 0.3513435583106069 0.3474684534369375 PE O-16:1_18:1 4.475966148 3.066295476449065 4.195670113317016 1.8403422470868869 3.4585555561346095 3.112863324430168 PE O-16:1_18:2 0.5980338005232718 0.3917063911641456 0.5425458015013573 0.17918924745712966 0.3316639840704486 0.2990131558318361 PE O-16:1_20:3 0.1081983826992616 0.10575248143803677 0.15239877531039514 0 0 0.058664504282996015 PE O-16:1_20:4 2.0318473560053003 2.3677218323366325 2.5653726819233116 1.4972614037456469 1.5288820999151058 1.6911925483084724 PE O-16:1_20:5 0.3522046949839981 0.35099979054670155 0.4355836683844516 0.14396769763140094 0.1917480994796004 0.18795501643237583 PE O-16:1_22:6 1.1070200744061822 1.3961748340100326 1.446001670183849 0.974723515 0.87839674 0.8523462767299214 PE O-17:0_18:1 0.17969395078889214 0.11984421333795586 0.12879004652753803 0.10456418840195991 0.17520765227825422 0.15644176203185683 PE O-18:0_16:0 0.082021833 0 0.066013555 0.092300045 0.18741382640615514 0.16321447060871555 PE O-18:0_18:1 4.262419128319868 2.356206124804052 3.023614592915567 2.5485187349395435 6.251171759973358 5.176991096192886 PE O-18:0_20:1 0.4142398546767816 0.265727919 0.3648508748390479 0.32042896209004496 0.718918772 0.6146232949898405 PE O-18:0_20:3 0.2454842211024459 0.18601393520352275 0.2441354442497693 0.12693965864777298 0.2676329330751445 0.21344766928460024 PE O-18:0_20:4 1.4857159964639273 1.2172196963284752 1.3430774086527288 1.1471875500899935 2.0245384447723778 1.852176558372842 PE O-18:0_22:4 0.1566989716243158 0.15573222490312064 0.14532585849846047 0.2644694971020115 0.6655660051869735 0.603294907 PE O-18:0_22:5 0.5818013526808227 0.53400003439924415 0.5653935411685981 0.6054238735559738 0.9923886791269817 0.8927904244972957 PE O-18:0_22:6 1.1679880562769873 1.1225093415646683 1.2060704253648802 1.0601324804676857 1.6533661493954253 1.512865032428687 PE O-18:1_16:1 1.0633577246421575 0.7488057971367228 0.8778415938105395 0.40057957939401573 0.5873850844931591 0.9644683544700866 PE O-18:1_18:1 4.5885140177810095 2.4967861736789874 3.4340898399931605 2.0023286430999265 5.152632714967698 4.177380227867148 PE O-18:1_20:1 0.7493568691655084 0.44276925732932576 0.6257641967969988 0.4892375231505016 0.9151024023844588 0.8161360346266434 PE O-18:1_20:4 0.5928895443397254 0.6929285205197547 0.804204438 0.5646816668849841 0.8997562568101025 0.847388766 PE O-18:1_22:4 0.087864569 0 0 0.080773902 0.11875395867982722 0.10140739780001323 PE O-18:1_22:5 0 0 0 0.3801639411125174 0.48871593247070205 0.5823634289257934 PE O-18:1_22:6 0.34841153940782843 0.45338809361455634 0.46907885785963155 0.36194267271355407 0.4991100910480496 0.5115796940507664 PE O-18:2_18:1 1.612711926812169 1.1352840043607604 1.547979299459212 0.7087699799951446 1.529541356316095 1.285237961443119 PE O-18:2_22:6 0.36448991328088404 0.38513100433604897 0.44208335659159104 0.31036309169098286 0.3898276388252725 0.3677394394134817 PE O-20:1_18:1 0.1265594414347869 0.091511406 0.12720121117461208 0.072865929 0.16721203955755723 0.14166983067728867 PE O-22:1_20:4 0.066354415 0.044371859 0.054624934 0.025748800444604755 0.038378815 0.036241598 PE O-24:1_18:1 0.19824781383163678 0.080120799 0.10416997620367581 0.040460229 0.1202625464529519 0.088044364 PE O-24:1_20:4 0.1281898166885633 0.066999386 0.076819838 0.017233616389547592 0.04092154 0.036315423 PG 16:0_18:1 0.2034716156261802 0.393099924 0.30010036981447297 0.3321858840183557 0.2824824870860293 0.25849850591806295 PG 18:0_18:1 0.17460974594973017 0.24859188798926993 0.20085525449948496 0.13443641438347764 0.18135196500071754 0.1483946506867134 PG 18:1_18:1 0.039058227452060425 0.040746956 0.04780454 0.039687819 0.052967968 0.037241256 PS 16:0_16:0 0.032522327824176966 0.080345635 0.047252221077222864 0.10843637952807282 0.056258558 0.072137063 PS 16:0_16:1 0.6339281617432427 1.541020927775127 1.1167205335503159 0.43379789097291815 0.3226339374165244 0.35575472878590403 PS 16:0_18:1 7.420040999541383 10.009719853081343 9.371907643876893 4.820736843280812 5.325243368546406 4.888583938544943 PS 16:1_18:1 0.8182746634749636 1.0500709923049991 1.0130756699987227 0.45871196884877247 0.5406682103062035 0.5145017419694943 PS 18:0_16:1 0 0 0 0.6768554959534395 0 1.1192770995213275 PS 18:0_18:1 20.070060357535457 18.12387883650275 19.631509852878715 12.916793830875337 21.51149862403039 19.015372666377658 PS 18:0_20:3 0.9728083091781898 1.2133339277065631 1.0890567610690178 0.6214469898965338 0.8485515847961913 0.7006581250543299 PS 18:0_20:4 0.7464431319626029 0.5613782879223107 0.6299566811511303 0.3014706628321376 0.6162929022787254 0.47485468585185453 PS 18:0_22:5 1.882180265962861 2.1034893929029943 2.052669745550073 1.2783266406015459 2.2212163852157594 1.7816022677515226 PS 18:0_22:6 2.9067780040961373 1.8015562227412854 2.4412571630875974 1.1687730907422527 2.8044873466860305 2.030799289721898 PS 18:1_18:1 6.918289382274547 6.621871943820799 7.379912296205389 4.445332007247616 7.891884311893721 6.657794734082225 PS 18:1_19:1 0.28438179817446857 0.20609151 0.2741190938132222 0.15220706381319302 0.27173572933778806 0.269923619 PS 18:1_20:1 1.8103362077424807 0 2.097910636504441 1.3794146236647888 0 1.474069660528251 PS 18:1_22:1 2.171020941917808 2.3129570741641854 2.7188553640830606 1.7968658445658785 2.4229816948945158 1.9976263486475632 PS 18:1_24:1 1.8523106064344774 2.2280074390193265 2.408504116597665 1.55533964 1.794217812666128 1.5193220690355274 PS 18:1_26:1 0.28162828593989847 0.5068558583616485 0.42305947930755683 0.21338782930030697 0.13454857463173286 0.12461117608692479 PS 22:0_18:1 1.7192854759701297 1.8020967006546509 1.9154335783580878 1.0732599566333005 1.677275489729271 1.2853715498385163 PS 24:0_18:1 1.5622445190547378 1.7237027491675916 1.7844651339466615 0.9815256784564029 1.3815363631257023 1.0610670887521558 PS 40:6 0 0.1983614143798467 0.18948333307756318 0.11023906219301124 0 0 PS O-18:0_18:1 0.3369430377906696 0.4022173849441963 0.42680650347315335 0.22524242630319977 0.3455524909358537 0.2794261184596526 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name Average Rt(min) Average Mz CL 14:0_16:1_18:1_18:1 18.778 686.47174 CL 16:0_16:1_18:1_18:1 19.842 700.48602 CL 16:0_18:0_18:0_22:0 8.495 745.55054 CL 16:0_18:1_16:0_18:1 20.787 1403.99365 CL 16:0_18:1_16:1_18:1 19.902 1401.98328 CL 16:0_18:1_18:1_18:1 20.81 1430.01196 CL 16:1_16:1_16:1_18:1 17.976 685.46362 CL 16:1_16:1_18:1_18:1 18.883 699.47974 CL 16:1_18:1_16:1_18:1 18.934 1399.96289 CL 16:1_18:1_16:1_18:2 18.123 1397.94885 CL 16:1_18:1_18:1_18:1 19.954 1427.996 CL 16:1_18:1_18:1_18:2 19.107 1425.97815 CL 18:1_18:1_18:1_18:1 20.852 1456.02856 CL 18:1_18:1_18:1_18:2 20.127 1454.00977 CL 24:0_18:1_18:1_18:1 10.301 770.57007 PC 14:0_14:0 8.495 722.49707 PC 14:0_16:0 9.756 750.52942 PC 14:0_16:1 8.683 748.51306 PC 15:0_16:1 9.221 762.52991 PC 16:0_16:0 11.012 778.55914 PC 16:0_16:1 9.93 776.54401 PC 16:0_17:1 10.543 790.56152 PC 16:0_18:0 12.274 806.59265 PC 16:0_18:1 11.16 804.57739 PC 16:0_20:4 9.995 826.55975 PC 16:0_20:5 9.19 824.54425 PC 16:0_22:6 9.697 850.55963 PC 16:0_24:1 14.608 888.67084 PC 16:0_26:1 15.909 916.70093 PC 16:1_16:1 8.863 774.52881 PC 16:1_17:1 9.421 788.54517 PC 16:1_18:1 10.053 802.55969 PC 16:1_18:2 9.162 800.54626 PC 16:1_20:4 8.927 824.54462 PC 16:1_22:6 8.615 848.54541 PC 17:0_18:1 11.759 818.59393 PC 17:1_18:1 10.669 816.57764 PC 18:0_18:1 12.396 832.60663 PC 18:0_20:4 11.256 854.59131 PC 18:0_22:5 11.31 880.60681 PC 18:0_22:6 10.924 878.5932 PC 18:1_18:1 11.279 830.59204 PC 18:1_18:2 10.339 828.57812 PC 18:1_19:1 11.849 844.60822 PC 18:1_20:1 12.434 858.6217 PC 18:1_20:2 11.485 856.60645 PC 18:1_20:3 10.651 854.59155 PC 18:1_20:4 10.098 852.57764 PC 18:1_21:1 13.006 872.64008 PC 18:1_22:1 13.567 886.65442 PC 18:1_22:2 12.695 884.63879 PC 18:1_22:4 11.03 880.60657 PC 18:1_22:5 10.191 878.5932 PC 18:1_22:6 9.78 876.57819 PC 18:1_24:1 14.679 914.68585 PC 18:1_24:2 13.702 912.67285 PC 18:1_26:1 15.871 942.71765 PC 18:1_26:2 14.762 940.70093 PC 18:1_28:1 17.09 970.75073 PC 18:2_18:2 9.46 826.56219 PC 19:0_18:1 12.903 846.62421 PC 26:0_18:1 17.208 944.73346 PC 34:0 14.39 806.59796 PC 40:4 11.824 882.62213 PC O-16:0_14:0 10.561 736.55035 PC O-16:0_16:0 11.846 764.58234 PC O-16:0_16:1 10.732 762.56653 PC O-16:0_18:1 11.957 790.59674 PC O-16:0_20:4 10.777 812.58148 PC O-16:0_20:5 9.927 810.5672 PC O-16:0_22:4 11.428 840.61389 PC O-16:1_14:0 10.374 734.5343 PC O-16:1_16:0 11.651 762.56616 PC O-16:1_16:1 10.499 760.5495 PC O-16:1_18:1 11.754 788.58099 PC O-16:2_18:0 10.879 788.58136 PC O-18:0_16:0 13.1 792.6142 PC O-18:0_18:1 13.194 818.62823 PC O-18:0_20:4 12.041 840.61401 PC O-18:0_22:5 12.098 866.62805 PC O-18:0_22:6 11.72 864.61224 PC O-18:1_18:1 12.055 816.61426 PC O-18:1_22:4 11.799 866.62866 PC O-18:1_22:6 10.519 862.59564 PC O-18:2_18:1 11.823 814.59796 PC O-20:0_18:1 14.359 846.66119 PC O-20:1_18:1 13.214 844.64441 PC O-22:0_16:0 15.531 848.67474 PC O-22:1_18:1 14.365 872.67474 PC O-24:1_16:0 15.602 874.69208 PC O-24:1_18:1 15.585 900.70764 PC O-26:1_16:0 16.829 902.724 PC O-26:1_18:1 16.805 928.74042 PE 14:0_16:1 8.925 660.46106 PE 16:0_16:0 11.292 690.50842 PE 16:0_16:1 10.184 688.4917 PE 16:0_18:1 11.438 716.52515 PE 16:0_20:4 10.265 738.50739 PE 16:1_16:1 9.104 686.47644 PE 16:1_18:1 10.332 714.50909 PE 16:1_18:2 9.383 712.49347 PE 16:1_20:4 9.198 736.4942 PE 17:0_18:1 12.076 730.54089 PE 17:1_18:1 10.945 728.52368 PE 18:0_18:1 12.642 744.55505 PE 18:0_20:1 13.726 772.58826 PE 18:0_20:3 12.389 768.55573 PE 18:0_20:4 11.528 766.53821 PE 18:0_20:5 10.741 764.52332 PE 18:0_22:4 12.368 794.57074 PE 18:0_22:5 11.574 792.5556 PE 18:0_22:6 7.686 790.54065 PE 18:1_18:1 11.554 742.53918 PE 18:1_18:2 10.603 740.5235 PE 18:1_19:1 12.104 756.55463 PE 18:1_20:1 11.256 770.57111 PE 18:1_20:3 10.938 766.54126 PE 18:1_20:4 10.403 764.52319 PE 18:1_20:5 9.594 762.50696 PE 18:1_21:1 13.208 784.58728 PE 18:1_22:1 13.759 798.60376 PE 18:1_22:2 12.89 796.58777 PE 18:1_22:5 8.547 790.54083 PE 18:1_22:6 10.03 788.52423 PE 18:1_24:1 14.85 826.63251 PE 18:1_24:2 13.884 824.61743 PE 18:1_26:1 15.992 854.66461 PE 22:0_18:1 14.918 800.61908 PE 22:0_20:4 13.836 822.60223 PE 22:1_20:4 12.715 820.5871 PE 24:0_18:1 16.086 828.64886 PE 24:0_20:4 14.964 850.63208 PE 24:1_20:4 13.79 848.61816 PE 26:0_18:1 17.327 856.68011 PE 26:1_20:4 14.932 876.64948 PE O-16:0_16:0 12.117 676.52832 PE O-16:0_16:1 11.019 674.51361 PE O-16:0_17:1 11.649 688.53015 PE O-16:0_18:1 12.238 702.5434 PE O-16:0_20:3 11.657 726.54614 PE O-16:0_20:4 11.076 724.5293 PE O-16:0_20:5 10.227 722.51331 PE O-16:0_22:3 12.839 754.57574 PE O-16:0_22:4 11.975 752.56085 PE O-16:0_22:5 11.125 750.54517 PE O-16:0_22:6 10.718 748.52948 PE O-16:1_16:1 10.826 672.49774 PE O-16:1_18:1 12.058 700.52844 PE O-16:1_18:2 10.945 698.51282 PE O-16:1_20:3 11.762 724.52924 PE O-16:1_20:4 10.864 722.51306 PE O-16:1_20:5 10.009 720.49805 PE O-16:1_22:6 10.54 746.51361 PE O-17:0_18:1 12.842 716.56036 PE O-18:0_16:0 13.3 704.55927 PE O-18:0_18:1 13.394 730.5752 PE O-18:0_20:1 14.491 758.60846 PE O-18:0_20:3 13.201 754.57562 PE O-18:0_20:4 12.309 752.56097 PE O-18:0_22:4 13.128 780.59216 PE O-18:0_22:5 12.354 778.57703 PE O-18:0_22:6 11.961 776.56067 PE O-18:1_16:1 11.104 700.52875 PE O-18:1_18:1 12.298 728.56067 PE O-18:1_20:1 13.4 756.59113 PE O-18:1_20:4 12.107 750.54535 PE O-18:1_22:4 12.874 778.5769 PE O-18:1_22:5 11.215 776.56262 PE O-18:1_22:6 11.752 774.5462 PE O-18:2_18:1 12.128 726.54578 PE O-18:2_22:6 10.607 772.52869 PE O-20:1_18:1 14.318 756.59106 PE O-22:1_20:4 13.448 806.60834 PE O-24:1_18:1 15.657 812.6546 PE O-24:1_20:4 14.557 834.63806 PG 16:0_18:1 10.009 747.51947 PG 18:0_18:1 11.181 775.55066 PG 18:1_18:1 10.128 773.53503 PS 16:0_16:0 9.825 734.49731 PS 16:0_16:1 8.797 732.48175 PS 16:0_18:1 9.981 760.51398 PS 16:1_18:1 8.935 758.49963 PS 18:0_16:1 6.698 760.51324 PS 18:0_18:1 11.172 788.54602 PS 18:0_20:3 10.606 812.54633 PS 18:0_20:4 10.089 810.53015 PS 18:0_22:5 10.142 836.54376 PS 18:0_22:6 9.787 834.52832 PS 18:1_18:1 10.077 786.52917 PS 18:1_19:1 10.667 800.54626 PS 18:1_20:1 11.229 814.5611 PS 18:1_22:1 12.427 842.59259 PS 18:1_24:1 13.522 870.62335 PS 18:1_26:1 14.672 898.65405 PS 22:0_18:1 13.567 844.60815 PS 24:0_18:1 14.763 872.64032 PS 40:6 9.094 834.52893 PS O-18:0_18:1 12.016 774.56616 METABOLITES_END #END