<?xml version="1.0"?>
<MSAcqMethod xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xsd="http://www.w3.org/2001/XMLSchema">
  <msInstrument>QQQ</msInstrument>
  <ionSource>EI</ionSource>
  <tuneFile>AminoMTBSTFA-HP5.eiex.tune.xml</tuneFile>
  <stopMode>ByChromatographTime</stopMode>
  <stopTime>25</stopTime>
  <solventDelay>4.5</solventDelay>
  <collisionGasOn>true</collisionGasOn>
  <sourceParameters>
    <sourceParameter>
      <id>SourceHeater</id>
      <posPolarityValue>240</posPolarityValue>
      <negPolarityValue>240</negPolarityValue>
    </sourceParameter>
  </sourceParameters>
  <isTimeFilterEnabled>false</isTimeFilterEnabled>
  <timeFilterPeakWidth>0.0133333337</timeFilterPeakWidth>
  <timeFilter>
    <activeCount>0</activeCount>
    <definition>
      <time>0</time>
      <peakWidth>0.0133333337</peakWidth>
    </definition>
    <definition>
      <time>10</time>
      <peakWidth>0.05</peakWidth>
    </definition>
  </timeFilter>
  <useGain>true</useGain>
  <timeSegments>
    <timeSegment>
      <index>1</index>
      <startTime>4.5</startTime>
      <isDataSaved>true</isDataSaved>
      <scanSegments>
        <scanSegment>
          <index>1</index>
          <scanType>MS1SIM</scanType>
          <scanElements>
            <scanElement>
              <index>1</index>
              <compoundName>Ala, Sar</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>260</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>2</index>
              <compoundName>Gly, ABA</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>246</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>3</index>
              <compoundName>Sar</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>232</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>4</index>
              <compoundName>Gly</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>218</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>5</index>
              <compoundName>ABA</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>172</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>6</index>
              <compoundName>Ala</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>158</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
          </scanElements>
        </scanSegment>
      </scanSegments>
    </timeSegment>
    <timeSegment>
      <index>2</index>
      <startTime>8.22</startTime>
      <isDataSaved>true</isDataSaved>
      <scanSegments>
        <scanSegment>
          <index>1</index>
          <scanType>MS1SIM</scanType>
          <scanElements>
            <scanElement>
              <index>1</index>
              <compoundName>aIle, Ile</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>302</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>25</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>2</index>
              <compoundName>Val</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>288</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>25</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>3</index>
              <compoundName>Leu, Norl</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>274</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>25</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>4</index>
              <compoundName>GABA</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>273.9</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>25</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>5</index>
              <compoundName>Pro</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>258</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>25</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>6</index>
              <compoundName>Leu, aIleu, Ile, Norl</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>200</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>25</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>7</index>
              <compoundName>Val</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>186</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>25</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>8</index>
              <compoundName>Pro</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>184</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>25</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>9</index>
              <compoundName>GABA</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>146.9</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>25</dwell>
              <gain>1</gain>
            </scanElement>
          </scanElements>
        </scanSegment>
      </scanSegments>
    </timeSegment>
    <timeSegment>
      <index>3</index>
      <startTime>11.72</startTime>
      <isDataSaved>true</isDataSaved>
      <scanSegments>
        <scanSegment>
          <index>1</index>
          <scanType>MS1SIM</scanType>
          <scanElements>
            <scanElement>
              <index>1</index>
              <compoundName>Thr</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>404</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>2</index>
              <compoundName>Ser</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>362</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>3</index>
              <compoundName>Phe</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>336</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>4</index>
              <compoundName>Met</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>320</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>5</index>
              <compoundName>Thr</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>303</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>6</index>
              <compoundName>Ser, Phe</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>302</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>7</index>
              <compoundName>Tau</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>296</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>8</index>
              <compoundName>Met</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>292</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>9</index>
              <compoundName>Tau</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>158</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>10</index>
              <compoundName>Tau</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>73</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
          </scanElements>
        </scanSegment>
      </scanSegments>
    </timeSegment>
    <timeSegment>
      <index>4</index>
      <startTime>13.92</startTime>
      <isDataSaved>true</isDataSaved>
      <scanSegments>
        <scanSegment>
          <index>1</index>
          <scanType>MS1SIM</scanType>
          <scanElements>
            <scanElement>
              <index>1</index>
              <compoundName>Asp</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>418</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>2</index>
              <compoundName>Hyp</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>416</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>3</index>
              <compoundName>Cys</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>406</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>4</index>
              <compoundName>Glu</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>330</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>5</index>
              <compoundName>Asp</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>316</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>6</index>
              <compoundName>Hyp</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>314</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>7</index>
              <compoundName>Cys</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>304</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>8</index>
              <compoundName>Orn</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>286</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>9</index>
              <compoundName>Glu</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>272</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>10</index>
              <compoundName>Orn</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>184</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
          </scanElements>
        </scanSegment>
      </scanSegments>
    </timeSegment>
    <timeSegment>
      <index>5</index>
      <startTime>16.4</startTime>
      <isDataSaved>true</isDataSaved>
      <scanSegments>
        <scanSegment>
          <index>1</index>
          <scanType>MS1SIM</scanType>
          <scanElements>
            <scanElement>
              <index>1</index>
              <compoundName>2-Aminoadipic acid</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>446</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>2</index>
              <compoundName>Lys, Gln</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>431</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>3</index>
              <compoundName>Asn</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>417</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>4</index>
              <compoundName>2-Aminoadipic acid</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>344</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>5</index>
              <compoundName>Gpr</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>343</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>6</index>
              <compoundName>Gln</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>329</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>7</index>
              <compoundName>Asn</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>302</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>8</index>
              <compoundName>Lys</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>300</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>9</index>
              <compoundName>2-Aminoadipic acid</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>286</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>10</index>
              <compoundName>Gpr</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>144</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
          </scanElements>
        </scanSegment>
      </scanSegments>
    </timeSegment>
    <timeSegment>
      <index>6</index>
      <startTime>18.1</startTime>
      <isDataSaved>true</isDataSaved>
      <scanSegments>
        <scanSegment>
          <index>1</index>
          <scanType>MS1SIM</scanType>
          <scanElements>
            <scanElement>
              <index>1</index>
              <compoundName>Tyr</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>466</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>2</index>
              <compoundName>Arg</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>442</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>3</index>
              <compoundName>His</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>440</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>4</index>
              <compoundName>Hly</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>430</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>5</index>
              <compoundName>Trp-2TBDMS</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>375</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>6</index>
              <compoundName>Arg</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>340</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>7</index>
              <compoundName>Hly</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>327</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>8</index>
              <compoundName>Tyr, Trp-2TBDMS</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>302</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>9</index>
              <compoundName>His</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>196</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>20</dwell>
              <gain>1</gain>
            </scanElement>
          </scanElements>
        </scanSegment>
      </scanSegments>
    </timeSegment>
    <timeSegment>
      <index>7</index>
      <startTime>19.1</startTime>
      <isDataSaved>true</isDataSaved>
      <scanSegments>
        <scanSegment>
          <index>1</index>
          <scanType>MS1SIM</scanType>
          <scanElements>
            <scanElement>
              <index>1</index>
              <compoundName>C-C</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>639</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>2</index>
              <compoundName>CTH</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>362</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>3</index>
              <compoundName>C-C</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>348</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>4</index>
              <compoundName>Trp-3TBDMS, CTH</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>302</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
            <scanElement>
              <index>5</index>
              <compoundName>Trp-3TBDMS</compoundName>
              <isISTD>false</isISTD>
              <ms1LowMz>244</ms1LowMz>
              <ms1Res>Unit</ms1Res>
              <dwell>30</dwell>
              <gain>1</gain>
            </scanElement>
          </scanElements>
        </scanSegment>
      </scanSegments>
    </timeSegment>
  </timeSegments>
  <instrumentCurves>
    <samplingRate>5</samplingRate>
  </instrumentCurves>
  <chromatograms>
    <chromatogram>
      <index>1</index>
      <chromType>TIC</chromType>
      <label>TIC</label>
      <offset>0</offset>
      <yRange>1E+07</yRange>
    </chromatogram>
    <defaultExtractionWindow>
      <minus>0.3</minus>
      <plus>0.7</plus>
    </defaultExtractionWindow>
  </chromatograms>
</MSAcqMethod>