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MB Sample ID: SA004558
Local Sample ID: | S26G |
Subject ID: | SU000106 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | Human Adipocytes |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000106 |
Subject Type: | Human cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | Human Adipocytes |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S26G | SA004558 | FL001256 | Human Adipocytes grown in the PRESENCE of SKOV3ip1 OvCa cells (Co-Culture) | Treatment |
S26G | SA004558 | FL001256 | 4 hours | Time Point |
Collection:
Treatment:
Sample Preparation:
Combined analysis:
Analysis ID | AN000139 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | Restek Corporation Rtx-5Sil MS |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco GC-TOF |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH000099 |
Chromatography Summary: | GC |
Methods Filename: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf |
Instrument Name: | Agilent 6890N |
Column Name: | Restek Corporation Rtx-5Sil MS |
Column Pressure: | 7.7 PSI (initial condition) |
Column Temperature: | 50 - 330C |
Flow Rate: | 1 ml/min |
Injection Temperature: | 50C ramped to 250C by 12C/s |
Sample Injection: | 0.5l |
Oven Temperature: | 50C for 1 min, then ramped at 20C/min to 330C, held constant for 5 min |
Transferline Temperature: | 230C |
Washing Buffer: | Ethyl Acetate |
Sample Loop Size: | 30 m length x 0.25 mm internal diameter |
Randomization Order: | Excel generated |
Chromatography Type: | GC |
MS:
MS ID: | MS000115 |
Analysis ID: | AN000139 |
Instrument Name: | Leco GC-TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |
Ion Source Temperature: | 250°C |
Ionization Energy: | 70eV |
Mass Accuracy: | Nominal |
Source Temperature: | 250°C |
Scanning Range: | 80-500 Da |
Acquisition Parameters File: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf |
Processing Parameters File: | Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf |