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MB Sample ID: SA006783
Local Sample ID: | CER220_274308_ML_19 |
Subject ID: | SU000141 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000141 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
CER220_274308_ML_19 | SA006783 | FL001663 | Serum | Tissue/Fluid |
Collection:
Collection ID: | CO000125 |
Collection Summary: | - |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000143 |
Sample Preparation:
Sampleprep ID: | SP000138 |
Sampleprep Summary: | Methanol: Water Extraction |
Sampleprep Protocol Filename: | NIH_WCMC_LaMerrill_Method_GaikwadLab__SteroidAnalysis_2013-14.docx |
Processing Method: | Homogenization and Solvent Removal w/ Speed Vac |
Processing Storage Conditions: | On Ice |
Extraction Method: | 1:1 Methanol: Water |
Extract Concentration Dilution: | 100mg or 1ml per 4ml 1:1 MeOH:H2O |
Extract Storage: | -80C |
Sample Resuspension: | 150ul CH3OH/H2O |
Organ: | Sprague-Dawley Maternal: Adrenal, liver, placenta, amniotic fluid Sprague-Dawley Fetal: Male and female brain, male and female liver |
Combined analysis:
Analysis ID | AN000204 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity HSS T3 (150 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters Xevo-TQ |
Ion Mode | POSITIVE |
Units | pg/ml |
Chromatography:
Chromatography ID: | CH000136 |
Chromatography Summary: | Targeted UPLC-MS/MS |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity HSS T3 (150 x 2.1mm,1.8um) |
Flow Gradient: | 0-2 min 100% A (Water 0.1% formic acid) 0% B (CH3CN 0.1 % formic acid), 2-4 min 80% A, 4-9mins 45% A, 9-11 mins 20% A, 11-12 mins 100% A |
Flow Rate: | 0.15 ml/min |
Sample Injection: | 10ul |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Analytical Time: | 12 mins |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000167 |
Analysis ID: | AN000204 |
Instrument Name: | Waters Xevo-TQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3.0 kV |
Collision Gas: | N2 |
Ionization: | Electrospray Ionization |
Source Temperature: | 150C |
Desolvation Gas Flow: | 600 L/h |
Desolvation Temperature: | 350C |