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MB Sample ID: SA011514
Local Sample ID: | KD_386165_2 |
Subject ID: | SU000272 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Wistar |
Gender: | Male |
Animal Animal Supplier: | Charles River Laboratories |
Animal Housing: | Division of Veterenary Resources, University of Miami |
Animal Light Cycle: | 12 hour day / light cycle |
Animal Feed: | Ad libitum |
Animal Water: | Ad libitum |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000272 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Wistar |
Gender: | Male |
Animal Animal Supplier: | Charles River Laboratories |
Animal Housing: | Division of Veterenary Resources, University of Miami |
Animal Light Cycle: | 12 hour day / light cycle |
Animal Feed: | Ad libitum |
Animal Water: | Ad libitum |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
KD_386165_2 | SA011514 | FL002871 | ITD+RH+Glucose | Treatment |
KD_386165_2 | SA011514 | FL002871 | Hypothalamus | sample type |
Collection:
Collection ID: | CO000263 |
Collection Summary: | Rats were euthanized under isoflurane anesthesia (30% O2 / 70% N2O) |
Sample Type: | Tissue |
Collection Method: | Hippocampus and Hypothalamus were disected out qucikly and were frozen in liquid nitrogen. |
Volumeoramount Collected: | Entire hippocampus and hypothalamus were collected. |
Storage Conditions: | Samples were stored at - 80 o C freezer and shipped to Metabolomics facility on dry ice. |
Collection Vials: | 1.5 ml tubes |
Storage Vials: | 1.5 ml tubes |
Collection Tube Temp: | RT at the time of sample collection |
Tissue Cell Identification: | Hippocampus and hypothalamus |
Tissue Cell Quantity Taken: | Entire hippocampus and hypothalamus were collected. |
Treatment:
Treatment ID: | TR000283 |
Treatment Summary: | Two groups (i) Insulin-treated diabetic + Recurrent hypoglycemia, (ii) Insulin-treated diabetic + Recurrent hypoglycemia (only insulin injection without hypoglycemia) + Glucose infusion to maintain glucose to baseline levels. |
Sample Preparation:
Sampleprep ID: | SP000277 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Brain_Tissue_Sampleprep.pdf |
Sampleprep Protocol Comments: | Appendix C_Protein_DNA_quant.pdf |
Organ: | Brain |
Combined analysis:
Analysis ID | AN000398 | AN000399 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak area | Peak Area |
Chromatography:
Chromatography ID: | CH000282 |
Methods Filename: | Metabolomics_LCMSProtocol.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 0.350mL/min-0.600mL/min |
Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000342 |
Analysis ID: | AN000398 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000343 |
Analysis ID: | AN000399 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |