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MB Sample ID: SA331590
Local Sample ID: | IL10_KO_HC8 |
Subject ID: | SU003169 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU003169 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Gender: | Male and female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
IL10_KO_HC8 | SA331590 | FL039211 | Healthy_IL10-/- | Group |
Collection:
Collection ID: | CO003162 |
Collection Summary: | Colons were harvested from euthanized mice and flash-frozen in liquid nitrogen. For all downstream analyses, the distal region of the colon (0.5-2 cm from rectum) was used, since it is where colitis is most pronounced in these models. |
Sample Type: | Colon |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR003178 |
Treatment Summary: | Acute colitis was induced in wild type (WT) C57BL/6 mice via addition of 1.25% DSS into the drinking water for seven days. To gain insight on chronic intestinal inflammation, we used C57BL/6 IL-10-/- mice that spontaneously develop chronic colon inflammation. This inflammation is not chemically induced, but rather is considered to be a result of a dysregulated immune system, which more resembles the clinical manifestation of chronic UC and CD patients and mimics a monogenic form of IBD20. In order to synchronize and accelerate the onset of chronic colitis, mice were exposed to 200 ppm piroxicam (Sigma-Aldrich ltd.) via supplementation in normal murine chow for 14 days. With the described experimental procedures, we obtained five groups of mice (10 mice per group) for further analysis: 1) Healthy WT; 2) Day 4 of DSS (pre-symptomatic); 3) Day 10 of DSS (acute colitis); 4) Healthy IL-10-/- (pre-symptomatic); and 5) Ill IL-10-/- (chronic colitis). |
Sample Preparation:
Sampleprep ID: | SP003175 |
Sampleprep Summary: | Frozen colon samples were trimmed (15–20 mg) to fit HR-MAS ZrO2 rotor insert capacity (50 μL). Each insert was filled with 2H2O containing 5.8 mM sodium trimethylsilyl [2,2,3,3-2H4]propionate (TMSP). Inserts were covered with plug and plug-restraining screw and inserted into the 4 mm rotor for HR-MAS probe. |
Analysis:
MB Sample ID: | SA331590 |
Analysis ID: | AN005008 |
Analysis Type: | NMR |
Num Factors: | 5 |
Num Metabolites: | 22 |
Units: | AU |
NMR:
NMR ID: | NM000274 |
Analysis ID: | AN005008 |
Instrument Name: | Bruker 600 MHz |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 600 MHz |
NMR Probe: | HR-MAS TXI 1H/13C/15N probe |
NMR Solvent: | H2O |
NMR Tube Size: | 4 mm rotor for HR-MAS probe |
Pulse Sequence: | CPMG |
Water Suppression: | water peak suppression-presaturation |
Temperature: | 277 K |
Number Of Scans: | 64 |
Dummy Scans: | 4 |
Acquisition Time: | 1.36s |
Spectral Width: | 12 KHz |
Num Data Points Acquired: | 32 k |
Line Broadening: | 1 |
Baseline Correction Method: | automatic |
Chemical Shift Ref Std: | doublet at 5.24 ppm |
Binned Increment: | 0.02 ppm |
Binned Data Excluded Range: | 4.5-5.5 ppm |