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MB Sample ID: SA340620

Local Sample ID:Sample10
Subject ID:SU003259
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Subject:

Subject ID:SU003259
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Sample10SA340620FL040285Acox1-LKOSample_ID
Sample10SA340620FL040285mouse serumSample source

Collection:

Collection ID:CO003252
Collection Summary:After collecting the whole blood of mice, allow the blood to clot by leaving at room temperature for 30 minutes. Then serum were collected by centrifugation at 2000 g for 10 minutes, and save at -80C
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR003268
Treatment Summary:WT and Acox1-LKO mice were maintained under constant temperature (24°C), circulating air and humidity (45-65%) with 12h:12h light/dark cycle. All mice had free access to chew diet and water. 10 weeks age mice were used for analysis.

Sample Preparation:

Sampleprep ID:SP003266
Sampleprep Summary:The lipids extraction was performed strictly following the SOP established based on a modified Folch liquid-liquid extraction protocol. Briefly, an aliquot of 4.5 μL of each sample was vortexed with 1.5 μL of internal standard solution and methanol, followed by adding dichloromethane and vortexing for another 20 s. A clean-up step was performed with water and 10 seconds of vortex. Samples were allowed to equilibrate at room temperature for 10 min and centrifuged at 16,000 g for 10 min at 4°C. An aliquot of the organic layer was evaporated to dryness with a nitrogen blowdown evaporator. The residue was re-suspended in 4.5 μL of NovaMT MixB, vortexed for 1 min, and diluted with 40.5 μL of NovaMT MixA

Combined analysis:

Analysis ID AN005155 AN005156
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Bruker Impact HD Bruker Impact HD
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH003903
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:42 °C
Flow Gradient:t = 0 min, 5% B; t = 10 min, 40% B; t = 18.8 min, 98% B; t = 20.5 min, 98% B.
Flow Rate:250 μL/min.
Solvent A:50% Methanol/40% Acetonitrile/10% Water; 10 mM Ammonium formate
Solvent B:95% Isopropyl alcohol/5% water; 10 mM Ammonium formate
Chromatography Type:Reversed phase

MS:

MS ID:MS004891
Analysis ID:AN005155
Instrument Name:Bruker Impact HD
Instrument Type:QTOF
MS Type:ESI
MS Comments:The acquisition rate was 1.44 Hz for MS acquisition and 4 – 10 Hz for MS/MS spectra acquisition, with an m/z range from 150 to 1500. For both positive and negative ionization. Intensity threshold:2500 cts for positive ionization. Lipid features were extracted and aligned using software LipidScreener 1.1.0
Ion Mode:POSITIVE
Capillary Voltage:4200 V
Collision Energy:10-70 eV
Dry Gas Flow:5.0 L/min
Dry Gas Temp:240°C
Nebulizer:1.2 Bar
  
MS ID:MS004892
Analysis ID:AN005156
Instrument Name:Bruker Impact HD
Instrument Type:QTOF
MS Type:ESI
MS Comments:The acquisition rate was 1.44 Hz for MS acquisition and 4 – 10 Hz for MS/MS spectra acquisition, with an m/z range from 150 to 1500. For both positive and negative ionization. Intensity threshold:1500 cts for negative ionization. Lipid features were extracted and aligned using software LipidScreener 1.1.0
Ion Mode:NEGATIVE
Capillary Voltage:4200 V
Collision Energy:10-70 eV
Dry Gas Flow:5.0 L/min
Dry Gas Temp:240°C
Nebulizer:1.2 Bar
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