Summary of Study ST000282

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000224. The data can be accessed directly via it's Project DOI: 10.21228/M8K599 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000282
Study TitlePilot Study 13C flux effects when RhoC or RhoA perturbed (13C BCs)
Study Type13C mass isotopomer analysis (flux studies)
Study SummaryRho-GTPases are small GTP-binding proteins that contribute to the epithelial-to-mesenchymal transition by regulating several cellular processes including organization of the actin cytoskeleton, cell motility, transcription, and cell proliferation. Overexpression of RhoC-GTPases (RhoC) in breast cancer has been implicated in poor disease prognosis due to increased cancer cells invasion, migration, and motility, which warranted its consideration as a therapeutic target for inhibiting breast cancer metastasis. Using silencing RNA (siRNA) molecules to knockdown RhoC expression is a promising approach to inhibit breast cancer metastases.
Institute
University of Michigan
DepartmentInternal Medicine
LaboratoryMerajver Lab
Last NameWynn
First NameMichelle
AddressUniversity Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Emailmlwynn@umich.edu
Phone734-647-3408
Submit Date2015-02-11
Num Groups5
Total Subjects16
Raw Data File Type(s)d
Analysis Type DetailGC-MS/LC-MS
Release Date2016-01-13
Release Version1
Michelle Wynn Michelle Wynn
https://dx.doi.org/10.21228/M8K599
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000224
Project DOI:doi: 10.21228/M8K599
Project Title:13C mass isotopomer analysis
Project Summary:Pilot studies before designing future experiments
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Merajver Lab
Last Name:Merajver
First Name:Sophia
Address:University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Email:smerajve@med.umich.edu
Phone:(734)936-6884

Subject:

Subject ID:SU000302
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line
SA012306S00017282MCF10A  | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012307S00017283MCF10A  | 13C Label:Glutamine | RhoC overexpression:yes | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012308S00017284MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012309S00017287MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes
SA012310S00017286MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no
SA012311S00017285MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:yes | RhoC knockdown:no | RhoC drug:no
SA012312S00017295SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012313S00017293SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012314S00017294SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes
SA012315S00017290SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012316S00017288SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012317S00017289SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes
SA012318S00017296SUM190 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012319S00017297SUM190 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no
SA012320S00017291SUM190 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012321S00017292SUM190 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000296
Collection Summary:-
Sample Type:Cell Line

Treatment:

Treatment ID:TR000316

Sample Preparation:

Sampleprep ID:SP000310
Sampleprep Summary:-
Sampleprep Protocol Filename:Fluxomics_analysis_protocol-2015-03-11.docx

Combined analysis:

Analysis ID AN000449 AN000450
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm,3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6530 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units Area Area normalized

Chromatography:

Chromatography ID:CH000320
Methods ID:AQM010
Methods Filename:QTOF-002-HILIC-35min-1mm.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001310
Methods ID:AQM012
Methods Filename:MAHMOUD-PALM_14ISOTOPE.M
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000390
Analysis ID:AN000449
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:QTOF-002-HILIC-35min-1mm.m.zip
Processing Parameters File:EX00403-flux-MassHunterQuant-GlyTCA-DataAnalysis-LCMS-Method.m.zip
  
MS ID:MS000391
Analysis ID:AN000450
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Acquisition Parameters File:MAHMOUD-PALM_14ISOTOPE.M.zip
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