Summary of Study ST000698
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000495. The data can be accessed directly via it's Project DOI: 10.21228/M82S4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000698 |
| Study Title | Metabolomics analysis of Sirt5 knockdown (KD) melanoma |
| Study Type | MS analysis |
| Study Summary | Determine what kind of metabolites changing with Sirt5 KD. Con stands for the different condition of media |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-19 |
| Num Groups | 3 |
| Total Subjects | 7 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000495 |
| Project DOI: | doi: 10.21228/M82S4Z |
| Project Title: | Analysis of Sirt5 knockdown (KD) melanoma |
| Project Type: | MS analysis |
| Project Summary: | Sirt5 nontageted metabolites analysis |
| Institute: | University of Michigan |
| Department: | UMich Pathology |
| Laboratory: | Lombard Lab |
| Last Name: | Beecher |
| First Name: | Chris |
| Address: | University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 |
| Email: | chrisbee@med.umich.edu |
| Phone: | 734-232-0815 |
Subject:
| Subject ID: | SU000721 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Condition |
|---|---|---|
| SA039452 | S00018257 | no | KD#1:no | KD#2:yes |
| SA039453 | S00018256 | no | KD#1:no | KD#2:yes |
| SA039454 | S00018258 | no | KD#1:no | KD#2:yes |
| SA039455 | S00018270 | no | KD#1:no | KD#2:yes |
| SA039456 | S00018269 | no | KD#1:no | KD#2:yes |
| SA039457 | S00018255 | no | KD#1:no | KD#2:yes |
| SA039458 | S00018271 | no | KD#1:no | KD#2:yes |
| SA039459 | S00018254 | no | KD#1:no | KD#2:yes |
| SA039460 | S00018272 | no | KD#1:no | KD#2:yes |
| SA039461 | S00018273 | no | KD#1:no | KD#2:yes |
| SA039462 | S00018268 | no | KD#1:yes | KD#2:no |
| SA039463 | S00018264 | no | KD#1:yes | KD#2:no |
| SA039464 | S00018265 | no | KD#1:yes | KD#2:no |
| SA039465 | S00018267 | no | KD#1:yes | KD#2:no |
| SA039466 | S00018249 | no | KD#1:yes | KD#2:no |
| SA039467 | S00018253 | no | KD#1:yes | KD#2:no |
| SA039468 | S00018252 | no | KD#1:yes | KD#2:no |
| SA039469 | S00018251 | no | KD#1:yes | KD#2:no |
| SA039470 | S00018250 | no | KD#1:yes | KD#2:no |
| SA039471 | S00018266 | no | KD#1:yes | KD#2:no |
| SA039451 | S00020150 | Pooled melanoma | KD#1:Pooled melanoma | KD#2:Pooled melanoma |
| SA039472 | S00018259 | yes | KD#1:no | KD#2:no |
| SA039473 | S00018247 | yes | KD#1:no | KD#2:no |
| SA039474 | S00018246 | yes | KD#1:no | KD#2:no |
| SA039475 | S00018245 | yes | KD#1:no | KD#2:no |
| SA039476 | S00018248 | yes | KD#1:no | KD#2:no |
| SA039477 | S00018244 | yes | KD#1:no | KD#2:no |
| SA039478 | S00018262 | yes | KD#1:no | KD#2:no |
| SA039479 | S00018261 | yes | KD#1:no | KD#2:no |
| SA039480 | S00018260 | yes | KD#1:no | KD#2:no |
| SA039481 | S00018263 | yes | KD#1:no | KD#2:no |
| Showing results 1 to 31 of 31 |
Collection:
| Collection ID: | CO000715 |
| Collection Summary: | - |
Treatment:
| Treatment ID: | TR000735 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000728 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A003_-_Untargeted_Metabolomics.pdf |
Combined analysis:
| Analysis ID | AN001083 | AN001084 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent | Agilent |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area normalized | peak area normalized |
Chromatography:
| Chromatography ID: | CH000762 |
| Instrument Name: | Agilent |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000978 |
| Analysis ID: | AN001083 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
| MS ID: | MS000979 |
| Analysis ID: | AN001084 |
| Instrument Name: | Agilent 6530 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A003_-_Untargeted_Metabolomics.pdf |
