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MB Sample ID: SA250457
| Local Sample ID: | GC-Pre-TBI-34 |
| Subject ID: | SU002597 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004107 | AN004108 | AN004109 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 7890A | Thermo TSQ Vantage HPLC-MS/MS | Thermo TSQ Vantage HPLC-MS/MS |
| Column | Agilent DB-5ms (30m x 0.25mm, 0.25um) | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | EI | ESI | ESI |
| MS instrument type | Single quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 5977 | Thermo TSQ Vantage | Thermo TSQ Vantage |
| Ion Mode | UNSPECIFIED | POSITIVE | NEGATIVE |
| Units | pmoles/l | pmoles/l | pmoles/l |
Chromatography:
| Chromatography ID: | CH003042 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB-5ms (30m x 0.25mm, 0.25um) |
| Column Temperature: | 280 |
| Flow Gradient: | The oven temperature was initially set to 70°C, held for 3min, and then increased to 310°C at a rate of 5°C/min, and finally kept for 10min. |
| Flow Rate: | 1.2 mL/min |
| Solvent A: | N/A |
| Solvent B: | N/A |
| Chromatography Type: | GC |
| Chromatography ID: | CH003043 |
| Chromatography Summary: | positive ion mode |
| Instrument Name: | Thermo TSQ Vantage HPLC-MS/MS |
| Column Name: | Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0~1 min, 0.1~10% B; 1-5 min, 10-40% B; 5-17 min, 40-100% B; 17-22min, 100% B; 22-22.1 min, 100-10% B |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | water(0.1% formic acid ) |
| Solvent B: | acetonitrile(0.1% formic acid ) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003044 |
| Chromatography Summary: | negative ion mode |
| Instrument Name: | Thermo TSQ Vantage HPLC-MS/MS |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0-1min，0.1%B；1-3min，0.1-40%B；3-5min，40-100%B；5-10.5min，100%B；10.5-10.6min，0.1%B；10.6-18min |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | water(5 mM ammonium bicarbonate ) |
| Solvent B: | 95% methanol/water(5mM ammonium bicarbonate ) |
| Chromatography Type: | Reversed phase |
