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MB Sample ID: SA338108
| Local Sample ID: | QC11 |
| Subject ID: | SU003237 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO003230 |
| Collection Summary: | Day 4 gastruloids from 2 plates were collected (approximately 45-48 gastruloids, per treatment, per biological replicate) (N, biological replicate = 4) into ice cold PBS in a clean glass petri dish, washed once and transferred to another glass petri dish on ice containing cold PBS. Gastruloids were collected, briefly centrifuged to settle them down and the PBS was completely removed. Gastruloids were snap-frozen in liquid nitrogen. To count the number of cells in each gastruloid, 5 gastruloids from each batch and each treatment, were collected, trypsinised for 1 min in 50 µl of 0.05% trypsin (25300054, ThermoFisher Scientific) and neutralised using 950 µl of warm N2B27. Cells were immediately counted using the Neubauer haemocytometer chamber. |
| Collection Protocol Filename: | Sample_Collection_CD.pdf |
| Sample Type: | Embryonic cells |
| Storage Conditions: | Described in summary |
