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MB Sample ID: SA339221
| Local Sample ID: | GF6 |
| Subject ID: | SU003248 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl/6J |
| Age Or Age Range: | 8-10 weeks |
| Weight Or Weight Range: | 20-29g |
| Height Or Height Range: | - |
| Gender: | Male |
| Animal Animal Supplier: | Taconic |
| Animal Housing: | Gnotobiotic isolators/conventional housing |
| Animal Light Cycle: | 12/12 |
| Animal Feed: | RM1A (P) and RM3A (P) from Special Diet Services |
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Collection:
| Collection ID: | CO003241 |
| Collection Summary: | The acute restraint stress procedure was performed using a clean perforated polypropylene screw-cap 50 mL conical tubes. Cages were randomly assigned to either non-stress or stress groups. Each mouse that underwent stress was placed into the 50 mL tube and restrained for 15 minutes. After 15 minutes of restraint stress, mice were removed from the restrainer and either transported immediately to the cull room or returned to their home cage and left undisturbed for 45 minutes. To control for the variable of transport stress, mice from the non-stress control group were placed into new cages containing fresh bedding and transported the same distance before entering the cull room. Upon entering the cull room, mice were immediately decapitated, trunk blood collected, and tissues harvested. Caecal contents and intestinal samples were manually dissected and stored in PCR-grade microfuge tubes at -80°C until analyses. |
| Sample Type: | Cecum |
| Storage Conditions: | -80? |
