Summary of Study ST000282

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000224. The data can be accessed directly via it's Project DOI: 10.21228/M8K599 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000282
Study TitlePilot Study 13C flux effects when RhoC or RhoA perturbed (13C BCs)
Study Type13C mass isotopomer analysis (flux studies)
Study SummaryRho-GTPases are small GTP-binding proteins that contribute to the epithelial-to-mesenchymal transition by regulating several cellular processes including organization of the actin cytoskeleton, cell motility, transcription, and cell proliferation. Overexpression of RhoC-GTPases (RhoC) in breast cancer has been implicated in poor disease prognosis due to increased cancer cells invasion, migration, and motility, which warranted its consideration as a therapeutic target for inhibiting breast cancer metastasis. Using silencing RNA (siRNA) molecules to knockdown RhoC expression is a promising approach to inhibit breast cancer metastases.
Institute
University of Michigan
DepartmentInternal Medicine
LaboratoryMerajver Lab
Last NameWynn
First NameMichelle
AddressUniversity Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Emailmlwynn@umich.edu
Phone734-647-3408
Submit Date2015-02-11
Num Groups5
Total Subjects16
Raw Data File Type(s)d
Analysis Type DetailGC-MS/LC-MS
Release Date2016-01-13
Release Version1
Michelle Wynn Michelle Wynn
https://dx.doi.org/10.21228/M8K599
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line
SA012306S00017282MCF10A  | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012307S00017283MCF10A  | 13C Label:Glutamine | RhoC overexpression:yes | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012308S00017284MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012309S00017287MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes
SA012310S00017286MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no
SA012311S00017285MDA-MB-231 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:yes | RhoC knockdown:no | RhoC drug:no
SA012312S00017295SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012313S00017293SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012314S00017294SUM149 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes
SA012315S00017290SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012316S00017288SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012317S00017289SUM149 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:yes
SA012318S00017296SUM190 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012319S00017297SUM190 | 13C Label:Glucose | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no
SA012320S00017291SUM190 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:no | RhoC drug:no
SA012321S00017292SUM190 | 13C Label:Glutamine | RhoC overexpression:no | RhoA knockdown:no | RhoC knockdown:yes | RhoC drug:no
Showing results 1 to 16 of 16
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