Summary of Study ST002353
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001509. The data can be accessed directly via it's Project DOI: 10.21228/M8N71K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002353 |
| Study Title | Biomolecular condensates create phospholipid-enriched microenvironments (Part 3) |
| Study Type | Metabolomes of in vitro synthesized condensates |
| Study Summary | Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1. |
| Institute | Cornell University |
| Department | Department of Pharmacology |
| Laboratory | Dr. Samie Jaffrey |
| Last Name | Dumelie |
| First Name | Jason |
| Address | 1300 York Ave, LC-524, New York City, NY |
| jdumes98@gmail.com | |
| Phone | 6465690174 |
| Submit Date | 2022-11-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzdata.xml |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-03-01 |
| Release Version | 2 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Synthetic sample; Subject species: - (Factor headings shown in green)
| mb_sample_id | local_sample_id | metabolite_source |
|---|---|---|
| SA249779 | MED1 Library 100 nM Aqueous Sample 3 | 100 nM library | protein:MED1 | RNA:150 nM | fraction:aqueous |
| SA249780 | MED1 Library 100 nM Aqueous Sample 2 | 100 nM library | protein:MED1 | RNA:150 nM | fraction:aqueous |
| SA249781 | MED1 Library 100 nM Aqueous Sample 1 | 100 nM library | protein:MED1 | RNA:150 nM | fraction:aqueous |
| SA249782 | MED1 Library 100 nM Condensate Sample 1 | 100 nM library | protein:MED1 | RNA:150 nM | fraction:condensate |
| SA249783 | MED1 Library 100 nM Condensate Sample 3 | 100 nM library | protein:MED1 | RNA:150 nM | fraction:condensate |
| SA249784 | MED1 Library 100 nM Condensate Sample 2 | 100 nM library | protein:MED1 | RNA:150 nM | fraction:condensate |
| SA249785 | MED1 Library 100 nM Input Sample 3 | 100 nM library | protein:MED1 | RNA:150 nM | fraction:input |
| SA249786 | MED1 Library 100 nM Input Sample 1 | 100 nM library | protein:MED1 | RNA:150 nM | fraction:input |
| SA249787 | MED1 Library 100 nM Input Sample 2 | 100 nM library | protein:MED1 | RNA:150 nM | fraction:input |
| SA249770 | MED1 Library 10 uM Aqueous Sample 3 | 10 uM library | protein:MED1 | RNA:150 nM | fraction:aqueous |
| SA249771 | MED1 Library 10 uM Aqueous Sample 2 | 10 uM library | protein:MED1 | RNA:150 nM | fraction:aqueous |
| SA249772 | MED1 Library 10 uM Aqueous Sample 1 | 10 uM library | protein:MED1 | RNA:150 nM | fraction:aqueous |
| SA249773 | MED1 Library 10 uM Condensate Sample 2 | 10 uM library | protein:MED1 | RNA:150 nM | fraction:condensate |
| SA249774 | MED1 Library 10 uM Condensate Sample 1 | 10 uM library | protein:MED1 | RNA:150 nM | fraction:condensate |
| SA249775 | MED1 Library 10 uM Condensate Sample 3 | 10 uM library | protein:MED1 | RNA:150 nM | fraction:condensate |
| SA249776 | MED1 Library 10 uM Input Sample 3 | 10 uM library | protein:MED1 | RNA:150 nM | fraction:input |
| SA249777 | MED1 Library 10 uM Input Sample 2 | 10 uM library | protein:MED1 | RNA:150 nM | fraction:input |
| SA249778 | MED1 Library 10 uM Input Sample 1 | 10 uM library | protein:MED1 | RNA:150 nM | fraction:input |
| SA249761 | MED1 Library 1 uM Aqueous Sample 2 | 1 uM library | protein:MED1 | RNA:150 nM | fraction:aqueous |
| SA249762 | MED1 Library 1 uM Aqueous Sample 3 | 1 uM library | protein:MED1 | RNA:150 nM | fraction:aqueous |
| SA249763 | MED1 Library 1 uM Aqueous Sample 1 | 1 uM library | protein:MED1 | RNA:150 nM | fraction:aqueous |
| SA249764 | MED1 Library 1 uM Condensate Sample 1 | 1 uM library | protein:MED1 | RNA:150 nM | fraction:condensate |
| SA249765 | MED1 Library 1 uM Condensate Sample 3 | 1 uM library | protein:MED1 | RNA:150 nM | fraction:condensate |
| SA249766 | MED1 Library 1 uM Condensate Sample 2 | 1 uM library | protein:MED1 | RNA:150 nM | fraction:condensate |
| SA249767 | MED1 Library 1 uM Input Sample 2 | 1 uM library | protein:MED1 | RNA:150 nM | fraction:input |
| SA249768 | MED1 Library 1 uM Input Sample 1 | 1 uM library | protein:MED1 | RNA:150 nM | fraction:input |
| SA249769 | MED1 Library 1 uM Input Sample 3 | 1 uM library | protein:MED1 | RNA:150 nM | fraction:input |
| Showing results 1 to 27 of 27 |
