Summary of Study ST003150
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001958. The data can be accessed directly via it's Project DOI: 10.21228/M8MX5P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003150 |
Study Title | Impact of early-life exposure to a potent aryl hydrocarbon receptor ligand on gut microbiota and host glucose homeostasis in C57BL/6J male mice (Part II) |
Study Summary | This study aimed to explore the association between early-life exposure to a potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the microbiota, leading to impaired metabolic homeostasis later in life. This study utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and biochemical assays to analyze the gut microbiome composition and function, as well as the physiological and metabolic effects of early-life exposure to 2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in vitro was assessed using optical density (OD 600), flow cytometry, transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice exhibited disruption in the gut microbiome community structure and function, characterized by lower abundances of A. muciniphila, lower levels of cecal short chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes associated with early-life POP exposure were transferable to GF recipients in the absence of POP carry-over. In addition, AHR-independent interactions between POPs and the microbiota were observed, significantly affected the growth, physiology, gene expression, and metabolic activity of A. muciniphila, resulting in suppressed activity along the ILA pathway. |
Institute | Pennsylvania State University |
Department | Department of Veterinary and Biomedical Sciences |
Last Name | Koo |
First Name | Imhoi |
Address | 307B Life Science Building |
iuk41@psu.edu | |
Phone | 8148107425 |
Submit Date | 2024-03-28 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2024-04-30 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Sample source | Treatment |
---|---|---|---|
SA340917 | YT201026_14 | liver | TCDF |
SA340918 | YT201026_15 | liver | TCDF |
SA340919 | YT201026_16 | liver | TCDF |
SA340920 | YT200819_110 | liver | TCDF |
SA340921 | YT200819_109 | liver | TCDF |
SA340922 | YT200819_107 | liver | TCDF |
SA340923 | YT200819_108 | liver | TCDF |
SA340924 | YT201026_17 | liver | TCDF |
SA340925 | YT201026_19 | liver | TCDF |
SA340926 | YT210427_19 | liver | TCDF |
SA340927 | YT210427_20 | liver | TCDF |
SA340928 | YT210427_21 | liver | TCDF |
SA340929 | YT210427_18 | liver | TCDF |
SA340930 | YT210427_17 | liver | TCDF |
SA340931 | YT200819_106 | liver | TCDF |
SA340932 | YT210427_16 | liver | TCDF |
SA340933 | YT201026_18 | liver | TCDF |
SA340934 | YT200819_105 | liver | TCDF |
SA340935 | YT200819_11 | liver | TCDF |
SA340936 | YT200819_10 | liver | TCDF |
SA340937 | YT200819_09 | liver | TCDF |
SA340938 | YT200819_08 | liver | TCDF |
SA340939 | YT200819_13 | liver | TCDF |
SA340940 | YT200819_12 | liver | TCDF |
SA340941 | YT200819_06 | liver | vehicle |
SA340942 | YT200819_07 | liver | vehicle |
SA340943 | YT200819_05 | liver | vehicle |
SA340944 | YT200819_04 | liver | vehicle |
SA340945 | YT200819_03 | liver | vehicle |
SA340946 | YT200819_104 | liver | vehicle |
SA340947 | YT200819_103 | liver | vehicle |
SA340948 | YT200819_22 | liver | vehicle |
SA340949 | YT200819_02 | liver | vehicle |
SA340950 | YT200819_101 | liver | vehicle |
SA340951 | YT200819_102 | liver | vehicle |
SA340952 | YT200819_21 | liver | vehicle |
Showing results 1 to 36 of 36 |