Summary of Study ST002456
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001584. The data can be accessed directly via it's Project DOI: 10.21228/M8Z71C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002456 |
| Study Title | 1H NMR metabolomics applied to assess the metabolic response of Ruditapes philippinarum clams to sea warming and 17-α-ethinylestradiol exposure |
| Study Type | 1H NMR metabolomics to study the effects of warming conditions and exposure to 17-α-ethinylestradiol (EE2) on the polar metabolome of Ruditapes philippinarum clams |
| Study Summary | In this study, a comprehensive untargeted 1H NMR metabolomics strategy was applied to measure the metabolic impact of sea warming, in tandem with exposure to EE2, on Ruditapes philippinarum clams. The clams were exposed to five different EE2 concentrations: 0 (control group), 5, 25, 125 and 625 ng/L; either at 17 °C as control temperature or at 21 °C (representing a 4 °C increase, which corresponds to the worst-case warming scenario). The obtained data added important knowledge, unveiling individual metabolic effects of temperature rise and synergetic effects upon EE2 exposure, and paving the way for the definition of new metabolic markers for the monitoring of environmental stressors. |
| Institute | University of Aveiro |
| Department | CICECO – Aveiro Institute of Materials, Department of Chemistry |
| Laboratory | Metabolomics Group- CICECO |
| Last Name | Rodrigues |
| First Name | Joao A. |
| Address | University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal |
| joao.e.a.rodrigues@gmail.com | |
| Phone | 00351963481841 |
| Submit Date | 2023-01-26 |
| Num Groups | 10 |
| Total Subjects | 103 |
| Study Comments | This work was developed within the CICECO-Aveiro Institute of Materials project (UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020) financed by national funds through the FCT/MEC (PIDDAC). We are also grateful to the Portuguese National NMR Network (PTNMR), supported by FCT funds as the NMR spectrometer used is part of PTNMR and partially supported by Infrastructure Project No. 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL, and the FCT through PIDDAC). This work was also financially supported by the project BISPECIAl: BIvalveS under Polluted Environment and ClImate chAnge (POCI-01-0145-FEDER- 028425) funded by FEDER, through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI), and by national funds (OE), through FCT/MCTES. Mónica G. Silva benefited from Research Grant (MSc) (BI/CESAM/0043_2019/POCI-01-0145-FEDER-028425) under the project BISPECIAl: BIvalveS under Polluted Environment and ClImate change PTDC/CTA-AMB/28425/2017 (POCI-01-0145-FEDER-028425). |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2023-10-02 |
| Release Version | 1 |
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Project:
| Project ID: | PR001584 |
| Project DOI: | doi: 10.21228/M8Z71C |
| Project Title: | 1H NMR metabolomics applied to assess the metabolic response of Ruditapes philippinarum clams to sea warming and 17-α-ethinylestradiol exposure |
| Project Type: | 1H NMR metabolomics to study the effects of warming conditions and exposure to 17-α-ethinylestradiol (EE2) on the polar metabolome of Ruditapes philippinarum clams |
| Project Summary: | Hormones correspond to one of the most important classes of PhACs and are usually classified as endocrine-disrupting chemicals (EDCs), due to their ability to alter endocrine system functions, resulting in known adverse health effects on non-target organisms. Among the known EDCs, 17-α-ethinylestradiol (EE2) is characterized by high estrogenic potency, chemical stability, and a tendency to accumulate in aquatic biota. This hormone reaches the environment mainly through human and animal excretion, with conventional WWTP processes not fully managing its removal from treated effluents. Bivalves are excellent model organisms to assess the toxicity of several environmental stressors. Alongside the impacts of pollutants, organisms in coastal ecosystems are also subjected to climate change-related factors, such as temperature rise. Water temperature may increase up to 4 °C by 2100 and this is expected to severely impact several aspects of marine organisms’ biology. In addition, these environmental changes are also likely to affect the sensitivity of organisms to pollutants and, hence, pollutants toxicity. In this study, a comprehensive untargeted 1H NMR metabolomics strategy was applied to measure the metabolic impact of sea warming, in tandem with exposure to EE2, on Ruditapes philippinarum clams. The clams were exposed to different EE2 concentrations, either at 17 °C as control temperature or at 21 °C (representing a 4 °C increase, which corresponds to the worst-case warming scenario). The obtained data added important knowledge, unveiling individual metabolic effects of temperature rise and synergetic effects upon EE2 exposure, and paving the way for the definition of new metabolic markers for the monitoring of environmental stressors. |
| Institute: | University of Aveiro |
| Department: | CICECO – Aveiro Institute of Materials, Department of Chemistry |
| Laboratory: | Metabolomics group |
| Last Name: | Gil |
| First Name: | Ana M. |
| Address: | University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal |
| Email: | agil@ua.pt |
| Phone: | +351234370707 |
| Funding Source: | This work was developed within the CICECO-Aveiro Institute of Materials project (UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020) financed by national funds through the FCT/MEC (PIDDAC). We are also grateful to the Portuguese National NMR Network (PTNMR), supported by FCT funds as the NMR spectrometer used is part of PTNMR and partially supported by Infrastructure Project No. 022161 (co-financed by FEDER through COMPETE 2020, POCI and PORL, and the FCT through PIDDAC). This work was also financially supported by the project BISPECIAl: BIvalveS under Polluted Environment and ClImate chAnge (POCI-01-0145-FEDER- 028425) funded by FEDER, through COMPETE2020 - Programa Operacional Competitividade e Internacionalização (POCI), and by national funds (OE), through FCT/MCTES. Mónica G. Silva benefited from Research Grant (MSc) (BI/CESAM/0043_2019/POCI-01-0145-FEDER-028425) under the project BISPECIAl: BIvalveS under Polluted Environment and ClImate change PTDC/CTA-AMB/28425/2017 (POCI-01-0145-FEDER-028425). |
