Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA025379

Local Sample ID:	B32
Subject ID:	SU000508
Subject Type:	Animal
Subject Species:	Megalobrama amblycephala
Taxonomy ID:	75352
Species Group:	Fish

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Sample Preparation:

Sampleprep ID:	SP000515
Sampleprep Summary:	Each plasma sample (170 μL) was mixed with 340 μL phosphate buffer (45 mM, pH 7.47, 50% D2O) containing 0.9% NaCl in a 5 mm NMR tube and used directly for the NMR analysis. Liver tissues (about 50 mg) were homogenized in cold methanol and water (v/v=2:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). The supernatant (550uL) was collected after centrifugation (16 099 g, 4°C, 10 min). The extraction was repeated thrice using the same procedure. The supernatants collected from the above procedure were mixed together and freeze-dried after the removal of methanol in vacuo. Then the residue was dissolved in 600 μL of phosphate buffer (0.15 M, pH 7.38, 80% D2O) containing 0.001% TSP and 0.01% NaN3. The supernatant (550 μL) was transferred into a 5 mm NMR tube.

Sampleprep ID:

SP000515

Sampleprep Summary:

Each plasma sample (170 μL) was mixed with 340 μL phosphate buffer (45 mM, pH 7.47, 50% D2O) containing 0.9% NaCl in a 5 mm NMR tube and used directly for the NMR analysis. Liver tissues (about 50 mg) were homogenized in cold methanol and water (v/v=2:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). The supernatant (550uL) was collected after centrifugation (16 099 g, 4°C, 10 min). The extraction was repeated thrice using the same procedure. The supernatants collected from the above procedure were mixed together and freeze-dried after the removal of methanol in vacuo. Then the residue was dissolved in 600 μL of phosphate buffer (0.15 M, pH 7.38, 80% D2O) containing 0.001% TSP and 0.01% NaN3. The supernatant (550 μL) was transferred into a 5 mm NMR tube.