Return to study ST003120 main page
MB Sample ID: SA338103
| Local Sample ID: | CD041_001 |
| Subject ID: | SU003237 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP003244 |
| Sampleprep Summary: | Sample preparation: Metabolite extraction was performed by addition of 200 µL 80 % methanol (including 2 % (v/v) internal standards) and subsequent homogenization on dry ice via a bead beater (FastPrep-24; MP Biomedicals, CA, USA) at 6.0 m/s (5 x 30 s, 5 min pause time) using 1.0 mm zirconia/glass beads (Biospec Products, OK, USA). After centrifugation for 10 min at 15,000 g and 4 °C with a 5415R microcentrifuge (Eppendorf, Hamburg, Germany), supernatants were transferred and the remaining sample residues were reextracted with 200 µL acetonitrile:methanol:water (2:2:1, v/v) containing 1 % (v/v) formic acid using identical settings for homogenization and centrifugation. Corresponding supernatants of both extraction steps were combined and dried under a stream of nitrogen. Dried samples were reconstituted in 60 µL acetonitrile:methanol:water (2:2:1, v/v), vortexed for 5 min, centrifuged, and transferred to analytical glass vials. The LC-MS/MS analysis was initiated within one hour after the completion of the sample preparation. |
| Sampleprep Protocol Filename: | Sample_Collection_CD.pdf |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | Described in summary |
