Summary of Study ST002810
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001757. The data can be accessed directly via it's Project DOI: 10.21228/M8M13Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002810 |
Study Title | GAS2 encodes a 2-oxoglutarate dependent dioxygenase involved in ABA catabolism |
Study Summary | Liu et al. [1] recently reported the characterization of Arabidopsis thaliana GAS2 (Gain of Function in ABA-modulated Seed Germination 2), which was described as an enzyme that catalyzes the stereospecific hydration of GA12 to produce GA12 16, 17-dihydro-16α-ol (DHGA12). A second paper describes the conversion of GA12 to an unidentified product by GAS2 and also reports that this enzyme does not convert ABA [2]. However, as previously reported [3], we did not find any conversion of [17-14C]-labeled or [1-,7-,12-,18-14C4]-labeled GA12 by GAS2. Instead, we present here data showing that the recombinant GAS2 enzyme is capable of catabolising abscisic acid (ABA) to phaseic acid (PA) and further to a second product, putative 8’-carboxy-ABA (compound A; Fig. 1a) [4]. References: [1] Liu, H. et al. Biosynthesis of DHGA12 and its roles in Arabidopsis seedling establishment. Nat. Commun. 10, 1768 (2019). [2] Xiong, W. et al. The dioxygenase GIM2 functions in seed germination by altering gibberellin production in Arabidopsis. J. Integr. Plant Biol. 60, 276-291 (2018). [3] Lange, T. & Pimenta Lange, M. J. The Multifunctional Dioxygenases of Gibberellin Synthesis. Plant Cell Physiol. 61, 1869-1879 (2020). [4] Lange, T., Atiq, N., Pimenta Lange. GAS2 encodes a 2-oxoglutarate dependent dioxygenase involved in ABA catabolism. bioRxiv, doi: 10.1101/2022.11.16.516706 (2022). |
Institute | Technische Universität Braunschweig |
Department | Biochemie und Physiology der Pflanzen |
Laboratory | AG Lange |
Last Name | Lange |
First Name | Theo |
Address | Mendelssohnstr. 4 |
theo.lange@tu-bs.de | |
Phone | 00495313915880 |
Submit Date | 2023-08-01 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | GC-MS |
Release Date | 2023-10-11 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR002926 |
Treatment Summary: | Recombinant GAS2 and AtGA20ox1 were produced as previously described [5,6]. [5] Pimenta Lange, M. J. et al. Functional characterization of gibberellin oxidases from cucumber, Cucumis sativus L. Phytochemistry 90, 62-69 (2013). [6] Pimenta Lange, M. J. et al. Cucumber gibberellin 1-oxidase/desaturase initiates novel gibberellin catabolic pathways. J. Biol. Chem. 295, 8442-8448 (2020). |