Summary of project PR001899
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001899. The data can be accessed directly via it's Project DOI: 10.21228/M88147 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Project ID: | PR001899 |
| Project DOI: | doi: 10.21228/M88147 |
| Project Title: | Plasma instead of serum avoids critical confounding of clinical metabolomics studies by platelets |
| Project Summary: | Metabolomics is an emerging and powerful molecular profiling method supporting clinical investigations. Serum and plasma are commonly used without rational prioritization. Serum is collected after blood coagulation, a complex biochemical process involving active platelet metabolism. This may affect the metabolome and increase the variance as platelet counts and function may vary substantially in individuals. A multi-omics approach systematically investigating the suitability of serum and plasma for clinical studies demonstrated that metabolites correlated well (n=461, R2=0.991), whereas lipid mediators (n=104, R2=0.906) and proteins (n=322, R2=0.860) differed substantially between specimen. Independently, analysis of platelet releasates identified most biomolecules significantly enriched in serum when compared to plasma. A prospective, randomized, controlled parallel group metabolomics trial with acetylsalicylic acid administered for 7 days demonstrated that the apparent drug effects significantly differ depending on analyzed specimen. Only serum analyses of healthy individuals suggested a significant downregulation of TXB2 and 12-HETE, which were specifically formed during coagulation in vitro. Plasma analyses reliably identified acetylsalicylic acid effects on metabolites and lipids occurring in vivo such as a decrease in polyunsaturated fatty acids. The present data suggests that plasma should be preferred above serum for clinical metabolomics studies as the serum metabolome may be substantially confounded by platelets. |
| Institute: | University of Vienna |
| Department: | Department of Analytical Chemistry |
| Laboratory: | Gerner lab |
| Last Name: | Hagn |
| First Name: | Gerhard |
| Address: | Währingerstraße 38, 1090 Vienna, Austria |
| Email: | gerhard.hagn@univie.ac.at |
| Phone: | +43 1 4277 52375 |
| Publications: | https://doi.org/10.1021/acs.jproteome.3c00761 |
Summary of all studies in project PR001899
| Study ID | Study Title | Species | Institute | Analysis(* : Contains Untargted data) | Release Date | Version | Samples | Download(* : Contains raw data) |
|---|---|---|---|---|---|---|---|---|
| ST003049 | Plasma instead of serum avoids critical confounding of clinical metabolomics studies by platelets (Part 2/3 - Eicosadomics of isolated platelets) | Homo sapiens | University of Vienna | MS | 2024-04-12 | 1 | 48 | Uploaded data (2G)* |
| ST003050 | Plasma instead of serum avoids critical confounding of clinical metabolomics studies by platelets (Part 1/3 - Plasma and serum eicosadomics) | Homo sapiens | University of Vienna | MS | 2024-04-12 | 1 | 120 | Uploaded data (14.5G)* |
| ST003069 | Plasma instead of serum avoids critical confounding of clinical metabolomics studies by platelets (Part 3/3 - Plasma and serum metabolomics) | Homo sapiens | University of Vienna | MS | 2024-04-12 | 1 | 72 | Uploaded data (9.1M)* |
