Summary of Study ST000153
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000153 |
| Study Title | Primary T Cell Noxa Knockdown (Donor 8)-II |
| Study Summary | Labeling of cells was carried out in triplicate with each sample containing 35e6. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 16 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol. All samples were immediately stored in -80°C. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-02-13 |
| Num Groups | 3 |
| Total Subjects | 16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 347 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-04-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000129 |
| Project DOI: | doi: 10.21228/M85C7W |
| Project Title: | Metabolic effects of Noxa |
| Project Summary: | labeled glutamine metabolomics on Noxa expressing cells |
| Institute: | University of Minnesota |
| Department: | Masonic Cancer Center |
| Laboratory: | Kelekar Lab |
| Last Name: | Kelekar |
| First Name: | Ameeta |
| Address: | 420 Delaware Street SE, Minneapolis, MN 55455 |
| Email: | ameeta@umn.edu |
| Phone: | 612-626-2358 |
Subject:
| Subject ID: | SU000172 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Subject Comments: | 16 subjects |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Glucose label |
|---|---|---|
| SA008390 | S00017304 | no | Glutamine label:no |
| SA008391 | S00017312 | no | Glutamine label:no |
| SA008392 | S00017313 | no | Glutamine label:yes |
| SA008393 | S00017298 | no | Glutamine label:yes |
| SA008394 | S00017303 | no | Glutamine label:yes |
| SA008395 | S00017305 | no | Glutamine label:yes |
| SA008396 | S00017302 | no | Glutamine label:yes |
| SA008397 | S00017300 | no | Glutamine label:yes |
| SA008398 | S00017299 | no | Glutamine label:yes |
| SA008399 | S00017301 | no | Glutamine label:yes |
| SA008400 | S00017311 | yes | Glutamine label:no |
| SA008401 | S00017310 | yes | Glutamine label:no |
| SA008402 | S00017308 | yes | Glutamine label:no |
| SA008403 | S00017306 | yes | Glutamine label:no |
| SA008404 | S00017307 | yes | Glutamine label:no |
| SA008405 | S00017309 | yes | Glutamine label:no |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000158 |
| Collection Summary: | - |
| Sample Type: | Cells |
Treatment:
| Treatment ID: | TR000178 |
Sample Preparation:
| Sampleprep ID: | SP000172 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Fluxomics_analysis_protocol-2015-03-11.docx |
Combined analysis:
| Analysis ID | AN000244 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1260 |
| Column | Phenomenex Luna NH2 (150 x 1mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | NEGATIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH000169 |
| Methods ID: | AQM010 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm-No_Insert.m |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS000194 |
| Analysis ID: | AN000244 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Acquisition Parameters File: | QTOF-002-HILIC-35min-1mm.m |
| Processing Parameters File: | EX00404-MassHunterQuant-LCMS-Flux-DataAnalysis-Method.m |
