Summary of Study ST000615

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000447. The data can be accessed directly via it's Project DOI: 10.21228/M88P55 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000615
Study TitleGC/MS measurement of sarcosine in urine samples
Study SummaryGC/MS measurement of sarcosine in urine samples from prostate cancer and case control patients in a group of ancestry verified African American and European Americans.
Institute
Baylor College of Medicine
Last NameSreekumar
First NameArun
AddressOne Baylor Plaza, Houston, TX, 77030, USA
EmailArun.Sreekumar@bcm.edu
Phone713-798-3305
Submit Date2017-05-19
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Arun Sreekumar Arun Sreekumar
https://dx.doi.org/10.21228/M88P55
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000447
Project DOI:doi: 10.21228/M88P55
Project Title:Prostate cancer disparity study on urine
Project Summary:LC/MS-MS measurement of inosine to adenosine ratio as well as GC/MS measurement of sarcosine relative values in urine samples from
Institute:Baylor College of Medicine
Last Name:Sreekumar
First Name:Arun
Address:One Baylor Plaza, Houston, TX, 77030, USA
Email:Arun.Sreekumar@bcm.edu
Phone:713-798-3305

Subject:

Subject ID:SU000638
Subject Type:Humans
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Humans; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Diagnosis
SA03408018112Case control | RACE:-
SA03408118106Case control | RACE:-
SA03408218137Case control | RACE:-
SA03408318140Case control | RACE:-
SA03408418154Case control | RACE:-
SA03408518102Case control | RACE:-
SA03408618091Case control | RACE:-
SA03408718056Case control | RACE:-
SA03408818062Case control | RACE:-
SA03408918072Case control | RACE:-
SA03409018077Case control | RACE:-
SA03409118156Case control | RACE:-
SA03409218174Case control | RACE:-
SA03409318415Case control | RACE:-
SA03409418277Case control | RACE:-
SA03409518469Case control | RACE:-
SA03409618489Case control | RACE:-
SA03409718661Case control | RACE:-
SA03409818265Case control | RACE:-
SA03409918261Case control | RACE:-
SA03410018054Case control | RACE:-
SA03410118225Case control | RACE:-
SA03410218240Case control | RACE:-
SA03410318250Case control | RACE:-
SA03410418162Case control | RACE:-
SA03410518098Case control | RACE:-
SA03410617962Case control | RACE:-
SA03410718004Case control | RACE:-
SA03410818010Case control | RACE:-
SA03410917902Case control | RACE:-
SA03411017893Case control | RACE:-
SA03411118035Case control | RACE:-
SA03411217848Case control | RACE:-
SA03411317879Case control | RACE:-
SA03411418013Case control | RACE:-
SA03411517817Case control | RACE:-
SA03411618014Case control | RACE:-
SA03411718030Case control | RACE:-
SA03411817122Prostate cancer | RACE:African American
SA03411917234Prostate cancer | RACE:African American
SA03412018331Prostate cancer | RACE:African American
SA03412118097Prostate cancer | RACE:African American
SA03412216710Prostate cancer | RACE:African American
SA03412318882Prostate cancer | RACE:African American
SA03412419049Prostate cancer | RACE:African American
SA03412518998Prostate cancer | RACE:African American
SA03412618901Prostate cancer | RACE:African American
SA03412718887Prostate cancer | RACE:African American
SA03412818850Prostate cancer | RACE:African American
SA03412918317Prostate cancer | RACE:African American
SA03413018020Prostate cancer | RACE:African American
SA03413118064Prostate cancer | RACE:African American
SA03413218073Prostate cancer | RACE:African American
SA03413317985Prostate cancer | RACE:African American
SA03413415298Prostate cancer | RACE:African American
SA03413517891Prostate cancer | RACE:African American
SA03413617897Prostate cancer | RACE:African American
SA03413718175Prostate cancer | RACE:African American
SA03413818918Prostate cancer | RACE:Caucasian
SA03413918018Prostate cancer | RACE:Caucasian
SA03414018262Prostate cancer | RACE:Caucasian
SA03414118083Prostate cancer | RACE:Caucasian
SA03414219034Prostate cancer | RACE:Caucasian
SA03414315862Prostate cancer | RACE:Caucasian
SA03414419169Prostate cancer | RACE:Caucasian
SA03414517721Prostate cancer | RACE:Caucasian
SA03414617587Prostate cancer | RACE:Caucasian
SA03414717585Prostate cancer | RACE:Caucasian
SA03414818542Prostate cancer | RACE:Caucasian
SA03414917024Prostate cancer | RACE:Caucasian
SA03415018063Prostate cancer | RACE:Caucasian
SA03415118613Prostate cancer | RACE:Caucasian
SA03415218061Prostate cancer | RACE:Caucasian
SA03415318742Prostate cancer | RACE:Caucasian
SA03415418734Prostate cancer | RACE:Caucasian
SA03415518830Prostate cancer | RACE:Caucasian
Showing results 1 to 76 of 76

Collection:

Collection ID:CO000632
Collection Summary:Urine from ancestry typed AA and EA prostate cancer patients were used for this analysis. Urine specimens were spun and the supernatant was stored at −70°C until analysis.
Sample Type:Urine

Treatment:

Treatment ID:TR000652
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP000645
Sampleprep Summary:Urine supernatants were diluted with methanol containing spiked labeled internal standards (d3-sarcosine and d3-alanine), and extracted overnight at 4°C, using 1:1 molar ratio of water/chloroform at room temperature, with constant shaking. The aqueous methanolic layer was recovered, dried and azeotroped twice by adding 100 mL dimethylformamide (DMF), mixed and dried using a speedvac for 30 minutes. Following this, 100 mL of DMF and N-methyl-N-tert-butylmethylsilyltrifluoroacetamide (MtBSTFA) + 1% t-butyl-dimethylchlorosilane were added and incubated at 60°C for 1 hr. The samples were resuspended in ethyl acetate and injected into a GC-MS.

Combined analysis:

Analysis ID AN000944
Analysis type MS
Chromatography type GC
Chromatography system Agilent
Column Agilent DB5-MS (15m)
MS Type ESI
MS instrument type Single quadrupole
MS instrument name Agilent
Ion Mode POSITIVE
Units Area

Chromatography:

Chromatography ID:CH000672
Chromatography Summary:During the course of the run, temperature was ramped from 40° to 300° C in a 16 minute period.
Instrument Name:Agilent
Column Name:Agilent DB5-MS (15m)
Chromatography Type:GC

MS:

MS ID:MS000840
Analysis ID:AN000944
Instrument Name:Agilent
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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