Summary of Study ST000290
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000232. The data can be accessed directly via it's Project DOI: 10.21228/M8P01G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000290 |
| Study Title | 2014 Biotron Experiment Metabolites |
| Study Type | Time course |
| Study Summary | Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Jon |
| Last Name | Stewart |
| First Name | Jon |
| Address | 102 Leigh Hall |
| jds2@chem.ufl.edu | |
| Phone | 352-846-0743 |
| Submit Date | 2015-02-19 |
| Num Groups | 3 |
| Total Subjects | 47 |
| Study Comments | Normal day / normal night (17 samples); Hot day / normal night (14 samples); Hot day / hot night (17 samples) |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000232 |
| Project DOI: | doi: 10.21228/M8P01G |
| Project Title: | Climate Change and Starch Yield in Maize Part 2 |
| Project Type: | MS study |
| Project Summary: | Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination. |
| Institute: | University of Florida |
| Department: | Chemistry |
| Laboratory: | Stewart |
| Last Name: | Stewart |
| First Name: | Jon |
| Address: | 126 Sisler Hall |
| Email: | jds2@chem.ufl.edu |
| Phone: | 846-0743 |
| Funding Source: | USDA |
Subject:
| Subject ID: | SU000310 |
| Subject Type: | Plant |
| Subject Species: | Zea mays |
| Taxonomy ID: | 4577 |
| Species Group: | Plant |
Factors:
Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)
| mb_sample_id | local_sample_id | Temperature |
|---|---|---|
| SA013170 | H_H_plant7_14DAP | Both hot day and hot night | Days after pollination:14 |
| SA013171 | H_H_plant18_14DAP | Both hot day and hot night | Days after pollination:14 |
| SA013172 | H_H_plant16_14DAP | Both hot day and hot night | Days after pollination:14 |
| SA013173 | H_H_plant8_14DAP | Both hot day and hot night | Days after pollination:14 |
| SA013174 | H_H_plant22_18DAP | Both hot day and hot night | Days after pollination:18 |
| SA013175 | H_H_plant17_18DAP | Both hot day and hot night | Days after pollination:18 |
| SA013176 | H_H_plant10_18DAP | Both hot day and hot night | Days after pollination:18 |
| SA013177 | H_H_plant24_22DAP | Both hot day and hot night | Days after pollination:22 |
| SA013178 | H_H_plant2_22DAP | Both hot day and hot night | Days after pollination:22 |
| SA013179 | H_H_plant15_22DAP | Both hot day and hot night | Days after pollination:22 |
| SA013180 | H_H_plant16_26DAP | Both hot day and hot night | Days after pollination:26 |
| SA013181 | H_H_plant14_26DAP | Both hot day and hot night | Days after pollination:26 |
| SA013182 | H_H_plant4_26DAP | Both hot day and hot night | Days after pollination:26 |
| SA013183 | H_H_plant21_30DAP | Both hot day and hot night | Days after pollination:30 |
| SA013184 | H_H_plant23_30DAP | Both hot day and hot night | Days after pollination:30 |
| SA013185 | H_H_plant3_30DAP | Both hot day and hot night | Days after pollination:30 |
| SA013186 | N_N_plant10_14DAP | Control | Days after pollination:14 |
| SA013187 | N_N_plant20_14DAP | Control | Days after pollination:14 |
| SA013188 | N_N_plant4_14DAP | Control | Days after pollination:14 |
| SA013189 | N_N_plant5_18DAP | Control | Days after pollination:18 |
| SA013190 | N_N_plant1_18DAP | Control | Days after pollination:18 |
| SA013191 | N_N_plant17_18DAP | Control | Days after pollination:18 |
| SA013192 | N_N_plant22_22DAP | Control | Days after pollination:22 |
| SA013193 | N_N_plant14_22DAP | Control | Days after pollination:22 |
| SA013194 | N_N_plant24_22DAP | Control | Days after pollination:22 |
| SA013195 | N_N_plant21_22DAP | Control | Days after pollination:22 |
| SA013196 | N_N_plant15_26DAP | Control | Days after pollination:26 |
| SA013197 | N_N_plant16_26DAP | Control | Days after pollination:26 |
| SA013198 | N_N_plant6_26DAP | Control | Days after pollination:26 |
| SA013199 | N_N_plant8_26DAP | Control | Days after pollination:26 |
| SA013200 | N_N_plant12_30DAP | Control | Days after pollination:30 |
| SA013201 | N_N_plant3_30DAP | Control | Days after pollination:30 |
| SA013202 | N_N_plant7_30DAP | Control | Days after pollination:30 |
| SA013203 | H_N_plant22_14DAP | Hot day | Days after pollination:14 |
| SA013204 | H_N_plant1_14DAP | Hot day | Days after pollination:14 |
| SA013205 | H_N_plant2_14DAP | Hot day | Days after pollination:14 |
| SA013206 | H_N_plant6_18DAP | Hot day | Days after pollination:18 |
| SA013207 | H_N_plant20_18DAP | Hot day | Days after pollination:18 |
| SA013208 | H_N_plant23_18DAP | Hot day | Days after pollination:18 |
| SA013209 | H_N_plant13_22DAP | Hot day | Days after pollination:22 |
| SA013210 | H_N_plant21_22DAP | Hot day | Days after pollination:22 |
| SA013211 | H_N_plant4_26DAP | Hot day | Days after pollination:26 |
| SA013212 | H_N_plant5_26DAP | Hot day | Days after pollination:26 |
| SA013213 | H_N_plant9_26DAP | Hot day | Days after pollination:26 |
| SA013214 | H_N_plant17_26DAP | Hot day | Days after pollination:26 |
| SA013215 | H_N_plant3_30DAP | Hot day | Days after pollination:30 |
| SA013216 | H_N_plant8_30DAP | Hot day | Days after pollination:30 |
| Showing results 1 to 47 of 47 |
Collection:
| Collection ID: | CO000304 |
| Collection Summary: | n/a |
| Sample Type: | Maize kernel |
| Collection Method: | manual removal from maize ear |
| Collection Location: | University of Wisconsin Biotron |
| Storage Conditions: | Frozen at -80C |
Treatment:
| Treatment ID: | TR000324 |
| Treatment Summary: | n/a |
| Plant Growth Location: | Wisconsin Biotron Facility |
| Plant Plot Design: | Random placement in growth facility |
| Plant Light Period: | 16 h per day |
| Plant Temp: | Normal day / normal night (28C day / 17C night; Hot day / normal night (38C / 17C); Hot day / hot night (38C / 28C) |
| Plant Watering Regime: | Fully watered |
| Plant Growth Stage: | Varied |
| Plant Metab Quench Method: | Frozen at -80C |
Sample Preparation:
| Sampleprep ID: | SP000318 |
| Sampleprep Summary: | n/a |
| Sampleprep Protocol Filename: | Maize_Tissue_sample_prep.pdf |
Combined analysis:
| Analysis ID | AN000462 | AN000463 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Scientific-Dionex Ultimate 3000 | Thermo Scientific-Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000329 |
| Methods Filename: | Instrument_Parameters.pdf |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 350 ul/min |
| Internal Standard: | Appendix A - Internal Standard Prep GLCMS.pdf |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000402 |
| Analysis ID: | AN000462 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Instrument_Parameters.pdf |
| MS ID: | MS000403 |
| Analysis ID: | AN000463 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | Instrument_Parameters.pdf |
