Summary of Study ST001168

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000780. The data can be accessed directly via it's Project DOI: 10.21228/M8VH63 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001168
Study TitleAlterations in fecal metabolic patterns are associated with atrial fibrillation
Study SummaryLittle evidence has been reported in characterizing the fecal alterations in metabolic patterns in atrial fibrillation (AF). We include the result of the global alterations that occur in the intestinal microbiota in a cohort of AF patients and matched controls based on a strategy of metabolomic analyses. Our findings characterize the disordered microbial metabolite profiles in AF.
Institute
Beijing Chaoyang Hospital
Last NameZuo
First NameKun
Address8th Gongtinanlu Rd, Chaoyang District, Beijing, China, 100020
Emailzuokun699@163.com
Phone86-10-15210511744
Submit Date2019-04-10
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2019-04-17
Release Version1
Kun Zuo Kun Zuo
https://dx.doi.org/10.21228/M8VH63
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000780
Project DOI:doi: 10.21228/M8VH63
Project Title:Alterations in fecal metabolic patterns are associated with atrial fibrillation
Project Summary:Little evidence has been reported in characterizing the fecal alterations in metabolic patterns in atrial fibrillation (AF). We include the result of the global alterations that occur in the intestinal microbiota in a cohort of AF patients and matched controls based on a strategy of metabolomic analyses. Our findings characterize the disordered microbial metabolite profiles in AF.
Institute:Beijing Chaoyang Hospital
Last Name:Zuo
First Name:Kun
Address:8th Gongtinanlu Rd,, Chaoyang District, Beijing, Beijing, 10020, China
Email:zuokun699@163.com
Phone:10-86-15210511744

Subject:

Subject ID:SU001233
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA080734af37A
SA080735af9A
SA080736af40A
SA080737af36A
SA080738af39A
SA080739af35A
SA080740af32A
SA080741af33A
SA080742af34A
SA080743af41A
SA080744af42A
SA080745af48A
SA080746af49A
SA080747af50A
SA080748af47A
SA080749af46A
SA080750af43A
SA080751af44A
SA080752af45A
SA080753af31A
SA080754af38A
SA080755af15A
SA080756af16A
SA080757af17A
SA080758af18A
SA080759af14A
SA080760af13A
SA080761af10A
SA080762af11A
SA080763af30A
SA080764af19A
SA080765af12A
SA080766af26A
SA080767af27A
SA080768af29A
SA080769af20A
SA080770af25A
SA080771af28A
SA080772af21A
SA080773af24A
SA080774af22A
SA080775af23A
SA080776FC29C
SA080777FC2C
SA080778FC17C
SA080779FC14C
SA080780FC13C
SA080781FC12C
SA080782FC9C
SA080783FC16C
SA080784FA4C
SA080785FA9C
SA080786FA7C
SA080787FA6C
SA080788FA13C
SA080789FA2C
SA080790FA3C
SA080791FA12C
SA080792FC10C
Showing results 1 to 59 of 59

Collection:

Collection ID:CO001227
Collection Summary:Fecal samples were collected from each participant, immediately frozen at −20 °C, transported on ice to the laboratory and then stored at −80 °C.
Sample Type:Feces

Treatment:

Treatment ID:TR001248
Treatment Summary:To explore how the host metabolic pattern alterations were impacted by the gut microbiota dysbiosis in AF patients, serum samples were collected and analyzed by high-throughput liquid chromatography-mass spectrometry (LC/MS).

Sample Preparation:

Sampleprep ID:SP001241
Sampleprep Summary:50 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 800 μL of methanol and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min at 4 °C. 200 μL of the supernatant was transferred into a vial for further analysis.

Combined analysis:

Analysis ID AN001931 AN001932
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Thermo Hypergod C18 (100 x 4.6mm,3um) Thermo Hypergod C18 (100 x 4.6mm,3um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Ion Mode POSITIVE NEGATIVE
Units feature area feature area

Chromatography:

Chromatography ID:CH001402
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Thermo Hypergod C18 (100 x 4.6mm,3um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001787
Analysis ID:AN001931
Instrument Name:Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100.
Ion Mode:POSITIVE
  
MS ID:MS001788
Analysis ID:AN001932
Instrument Name:Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:All metabolomic data were prepared for feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo). Using SIMCA-P software (Umetrics AB, Umea, Sweden), a multivariate Analysis (MVA) was performed. The exact molecular mass, ppm and ms/ms value of these compounds was used to identify the metabolites related to the featured peak in the Metlin database (http://metlin.scripps.edu). The score value indicated the matching rate was calculated by Compound Discoverer 2.0 software (Thermo) with max of 100.
Ion Mode:NEGATIVE
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