Summary of Study ST003061

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001907. The data can be accessed directly via it's Project DOI: 10.21228/M8714X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003061
Study TitleUntargeted metabolomics analysis of three tea cultivars.
Study Typeleaf tissue
Study SummaryTo compare flavonoid metabolism in three tea cultivars, namely Camellia sinensis cv. "Jinfenghuang" (JFH), "Fuyun 6" (FY6) and "Bantianyao" (BTY), fresh tea leaves were plucked, rapidly ground to fine powders in liquid nitrogen and subjected to extraction by 70% methanol. The extracts were analyzed by UPLC-Quadrupole Time-of-Flight Mass Spectrometry (UPLC-QTOFMS) using an ACQUITY UPLC system in tandem to a SYNAPT G2-Si QTOF mass spectrometer (Waters, Milford, MA, USA), following the previously described method. Raw data were processed in Progenesis QI (v2.4, Nonlinear Dynamics) with default settings for peak picking and alignment. Metabolites were annotated by searching against our in-house MS/MS spectral library and public databases.
Institute
Fujian Agriculture and Forestry University
Last NameYu
First NameXiaomin
AddressShangxia Dian Road No. 15, Fuzhou 350002, Fujian, China
Emailxmyu0616@sina.com
Phone(86)18649802306
Submit Date2024-01-28
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2024-02-05
Release Version1
Xiaomin Yu Xiaomin Yu
https://dx.doi.org/10.21228/M8714X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001907
Project DOI:doi: 10.21228/M8714X
Project Title:Characterization of CsUGT73AC15 as a Multifunctional Glycosyltransferase Impacting Flavonol Triglycoside Biosynthesis in Tea Plants
Project Summary:Flavonol glycosides, essential for the health benefits and astringent flavors of tea (Camellia sinensis), are abundant in tea leaves as diglycosides and triglycosides. However, the UDP-glycosyltransferases (UGTs) responsible for flavonol multi-glycosylation have remained elusive. In this study, integrated proteomic and metabolomic analyses were performed to identify potential UGTs involved in flavonol triglycoside biosynthesis. Among the tested recombinant enzymes, rCsUGT75AJ1 exhibited flavonoid 4ʹ-O-glucosyltransferase activity, while rCsUGT75L72 catalyzed glucosylation of flavonoids preferentially at the 3-OH position. Remarkably, rCsUGT73AC15 demonstrated substrate promiscuity and regioselectivity, enabling glucosylation of rutin at multiple positions as well as kaempferol 3-O-rutinoside at the 7-OH position. Expression analysis revealed an inverse correlation between the level of CsUGT73AC15 and rutin abundance across different cultivars, suggesting its involvement in flavonol triglycoside biosynthesis in planta. These findings advance the understanding of flavonol triglycoside biosynthesis in tea plants and provide a foundation for further in-vivo characterization of gene functions.
Institute:Fujian Agriculture and Forestry University
Last Name:Yu
First Name:Xiaomin
Address:Shang Xia Dian Road No. 15, Fuzhou, Fujian, 350002, China
Email:xmyu0616@sina.com
Phone:(86)18649802306
Publications:https://doi.org/10.1021/acs.jafc.4c03824

Subject:

Subject ID:SU003176
Subject Type:Plant
Subject Species:Camellia sinensis
Taxonomy ID:4442

Factors:

Subject type: Plant; Subject species: Camellia sinensis (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA331863BTY-1Bantianyao
SA331864BTY-2Bantianyao
SA331865BTY-3Bantianyao
SA331866FY6-3Fuyun 6
SA331867FY6-2Fuyun 6
SA331868FY6-1Fuyun 6
SA331869JFH-2Jinfenghuang
SA331870JFH-3Jinfenghuang
SA331871JFH-1Jinfenghuang
Showing results 1 to 9 of 9

Collection:

Collection ID:CO003169
Collection Summary:Mature leaves from the tea cultivars were plucked and flash-frozen in liquid nitrogen.
Sample Type:Leaves

Treatment:

Treatment ID:TR003185
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP003182
Sampleprep Summary:Leaves were ground into a fine powder using a pre-cooled mortar and pestle. Tea leaf powder (30 mg) was extracted with 1.0 mL of 70% methanol using ultrasonication for 20 min. After centrifugation, the supernatant was filtered through a PVDF filter (0.22 µm, Millipore).

Combined analysis:

Analysis ID AN005016
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity I-Class
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Synapt-G2-Si
Ion Mode NEGATIVE
Units Da

Chromatography:

Chromatography ID:CH003789
Instrument Name:Waters Acquity I-Class
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:1–7% B (0–2 min), 7–40% B (2–13 min), 40–60% B (13–17 min), immediately elevated to 99% B (17 min), held at 99% B (17–22 min)
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS004755
Analysis ID:AN005016
Instrument Name:Waters Synapt-G2-Si
Instrument Type:QTOF
MS Type:ESI
MS Comments:MassLynx software (ver 4.1) was used to operate the instrument. Progenesis QI software (ver 2.4) was used for peak picking, alignment and normalization.
Ion Mode:NEGATIVE
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