Summary of Study ST000482
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000366. The data can be accessed directly via it's Project DOI: 10.21228/M8R60S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000482 |
| Study Title | Free amino acids in CHEAR reference plasma |
| Study Type | Quantitative analysis |
| Study Summary | To determine the chemical composition of reference plasma sample for untargeted metabolomic analysis in CHEAR untargeted resources. |
| Institute | University of Minnesota |
| Department | Department of Food Science and Nutrition |
| Laboratory | Nutritional Metabolomics |
| Last Name | Chen |
| First Name | Chi |
| Address | 1334 Eckles Ave W, St Paul, MN 55108 |
| chichen@umn.edu | |
| Phone | 612-624-7704 |
| Submit Date | 2016-10-02 |
| Num Groups | 1 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Chear Study | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-12-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000366 |
| Project DOI: | doi: 10.21228/M8R60S |
| Project Title: | Reference plasma sample in CHEAR untargeted resource |
| Project Summary: | To determine the chemical composition of reference plasma sample for untargeted metabolomic analysis in CHEAR untargeted resources. |
| Institute: | University of Minnesota |
| Department: | Department of Food Science and Nutrition |
| Laboratory: | Nutritional Metabolomics |
| Last Name: | Chen |
| First Name: | Chi |
| Address: | 1334 Eckles Ave W, St Paul, MN 55108 |
| Email: | chichen@umn.edu |
| Phone: | 612-624-7704 |
| Funding Source: | NIEHS |
Subject:
| Subject ID: | SU000503 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | pooled |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample type |
|---|---|---|
| SA024581 | plasmaRef | Unknown |
| Showing results 1 to 1 of 1 |
Collection:
| Collection ID: | CO000497 |
| Collection Summary: | Human Plasma collected and processed by BioreclamationIVT. The plasma pool was created by combining recovered plasma obtained from 50 males and 50 females with an average age of 38.4 (range 19-72) and equal distribution of races. |
| Sample Type: | Plasma |
Treatment:
| Treatment ID: | TR000517 |
| Treatment Summary: | The plasma pool was created by combining recovered plasma obtained from 50 males and 50 females with an average age of 38.4 (range 19-72) and equal distribution of races. |
Sample Preparation:
| Sampleprep ID: | SP000510 |
| Sampleprep Summary: | Human reference plasma samples is derivatized with dansyl chloride (DC) prior to the LC-MS analysis. Briefly, 5 uL of sample or standard was mixed with 5 uL of of 100 µM indole-D5-L-tryptophan internal standard), 50 uL of 10 mM sodium carbonate, and 100 uL of DC solution (3 mg/mL in acetone). The mixture was incubated at 25°C for 15 min and centrifuged at 18,000 × g for 10 min, the supernatant was transferred into a HPLC vial for LC-MS analysis. |
| Processing Method: | derivatization, vortex, centrifugation |
| Processing Storage Conditions: | On ice |
Combined analysis:
| Analysis ID | AN000748 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Xevo-G2-S |
| Ion Mode | POSITIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH000536 |
| Chromatography Summary: | for detecting derivatized amino acids |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (50 x 2.1mm,1.7um) |
| Column Pressure: | 15000psi (Max) |
| Column Temperature: | 40 C |
| Flow Gradient: | 0-0.5min-0.5%B, 0.5-4min-gradient to 20%B, 4-8min-gradient to 95%B, 8-8.1min-gradient to 100%B, 8.1-9min-100%B, 9-9.1min gradient to 0.5%B, 9.1-10min-0.5%B |
| Flow Rate: | 0.5mL/min |
| Sample Injection: | 5 uL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 10min |
| Weak Wash Solvent Name: | 50% methanol in water |
| Weak Wash Volume: | 500 uL |
| Strong Wash Solvent Name: | 50% isopropanol, 25% acetonitrile and 25% water |
| Strong Wash Volume: | 500 uL |
| Sample Loop Size: | 20 uL |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000662 |
| Analysis ID: | AN000748 |
| Instrument Name: | Waters Xevo-G2-S |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Mass Accuracy: | <5 ppm |
